Rapid Identification of Yeasts

Rapid Identification

Two Alternative Approaches

Aim and Methodology

Topics to be covered

Saccharomyces species-specific

2011

Discovery of S. eubayanus*

S. eubayanus species-specific primers have been developed (228bp)

S. eubayanus with hybrid strains

Multiplex False Positives

Summary

Genus-specific primers

A. Use alignments (as for species-specific)

B. Look for regions within Saccharomyces ORFS that are missing in outgroups

Sec24p structure

C. Use genes absent in non-Saccharomyces species

Yeast Gene Order Browser (YGOB)

Results

Conclusions

Zygosaccharomyces species

Species-specific Colony PCR

Z. siamensis

Zygosaccharomyces Genus-specific Primers

Zygosaccharomyces Genus-specific Primers

Conclusions for Zygosaccharomyces

Level of detection in liquid cultures

Limitations of the Method

Overall Advantages

Conclusions

Acknowledgements

Identification by Molecular Methods

Hybrid detection in S. pastorianus

Hybrid detection in S. pastorianus

Hybrid detection in S. pastorianus

Hybrid detection in S. pastorianus

Current Saccharomyces species

Tests against a diverse global collectionS. paradoxus specific primers