1. ti s 1 Qu trnh phin m Prokaryote Tuesday, October 08, 2013 1

2. Tuesday, October 08, 2013 2 Thnh vin Khnh Vn Mai Hu Phng L Th Thu TrangNguyn Duy Hi Qua ng Cng Thnh 3. Tuesday, October 08, 2013 3 Ni dung I. Nhng c trng v mt ha hc ca s tng hp RNA II. Nguyn tc chung ca s tng hp RNA III. c im qu trnh phin m Prokaryote IV. C ch ca qu trnh phin m 4. Tuesday, October 08, 2013 4 I. Nhng c trng v mt ha hc ca s tng hp RNA - Cc thnh phn cu to RNA gm 4 loi nucleotide (A, U, G, C). - Trong RNA nhm 3OH ca nucleotide ny phn ng vi nhm 5P ca mt nucleotide khc. 5. Tuesday, October 08, 2013 5 - Trt t ca cc base nit trong mt phn t RNA c xc nh bi trt t base nit c trong khun mu DNA. I. Nhng c trng v mt ha hc ca s tng hp RNA - Hng tng trng ca chui polynucleotide l 5P 3OH 6. Tuesday, October 08, 2013 6 II. Nguyn tc chung ca s tng hp RNA - C 3 loi RNA u c tng hp t DNA. - Ch c 1 trong 2 mch ca DNA lm khun tng hp RNA. - S tng hp theo chiu 5P 3OH. 5P Nguyn liu tng hp l cc nucleotide triphosphate (ATP, GTP, UTP, CTP) 3OHA U A C U C G G A U A C 7. Tuesday, October 08, 2013 7 II. Nguyn tc chung ca s tng hp RNA - S tng hp RNA theo nguyn tc b sung (NTBS). - C s tham gia ca cc enzyme v khng cn tng hp on mi. 8. Tuesday, October 08, 2013 8 III. c im qu trnh phin m Prokaryote 9. Tuesday, October 08, 2013 9 - Mt mRNA cha thng tin ca nhiu gen cu trc (polycistron). - Qu trnh dch m xy ra ng thi vi qu trnh phin m. III. c im qu trnh phin m Prokaryote 10. Tuesday, October 08, 2013 10 IV. C ch ca qu trnh phin m Prokaryote Qu trnh tng hp RNA bao gm 3 giai on: 11. Tuesday, October 08, 2013 11 1. Khi u 12. Tuesday, October 08, 2013 12 1. Khi u - im gn ca RNA-polymerase trn promoter gm 2 trt t: + Trt t 6 base nit TATAAT (hp -10) + Trt t 6 base nit TTGACA (hp -35) - S tng hp bt u t im xut pht cch 7 base pha sau hp Pribnow. - Nucleotide th nht c t vo tng hp mt on RNA ngn 8-9 nucleotide. 13. Tuesday, October 08, 2013 13 2. Ko di - Khi phn t RNA t chiu di khong 10 nucleotide th nhn t sigma tch khi phc hp enzyme. - RNA-polymerase tho xon lin tc phn t DNA trn 1 chiu di khong 18 nucleotyde theo tin trin ca qu trnh sinh tng hp. 14. Tuesday, October 08, 2013 14 2. Ko di - Si RNA s tch dn khi mch khun DNA tr 1 on khong 12 nucleotyde. - Phn DNA c tho xon s c RNA-polymerase xon tr li sau . 15. Tuesday, October 08, 2013 15 16. Tuesday, October 08, 2013 16 3. Kt thc Khi RNA-polymerase t ti im tn cng ca chui th c phn t RNA v enzyme u c tch ra. C 2 kiu kt thc - Trn mch khun ca DNA xut hin trt t palindrome ngay trc mt on cha mt s A lin tip. 1. Kiu t kt thc 17. Tuesday, October 08, 2013 17 - on cui ca phn t RNA c th b gp khc to thnh cu trc cp tc. 3. Kt thc RNA polymerase dng li, RNA tch khi mch khun DNA 18. Tuesday, October 08, 2013 18 3. Kt thc 2. Kiu cn n s hin din ca mt loi protein - i theo RNA-polymerase n khi gp cu trc kp tc, RNA-polymerase tm dng 19. Tuesday, October 08, 2013 19 20. Tuesday, October 08, 2013 20 Cu hi cng c Cu 1: Cu trc no sau y c gi l palindrome? A. A C G C G G A T A C C C G C G T B. A C G C G G A T A C G C G C G T C. T A T A A T A G C A T T A A T A A T D. T A T A A T A G C A T T T A T A A T 21. Tuesday, October 08, 2013 21 Cu hi cng c Cu 2: Qu trnh phin m kt thc khi no? A. Khi on cui ca RNA c tng hp xut hin cu trc kp tc. B. Khi on cui ca RNA c tng hp xut hin cu trc kp tc cng vi A rU C.Khi RNA trt ht chiu di ca DNA D.Khi nhn t sigma ri khi enzyme RNA - polymerase 22. Tuesday, October 08, 2013 22 Cu hi cng c Cu 3: Polycistronic mRNA c hiu nh th no l ng? A. mRNA m ha cho nhiu chui polypeptide khc nhau. B. mRNA cha thng tin ca nhiu gen ni tip nhau. C.mRNA cha thng tin ca nhiu gen khc nhau. D.C A v C u ng 23. Tuesday, October 08, 2013 23 Cu hi cng c Cu 4: Cho trt t cc nucleotide trn mch b sung ca DNA nh sau: 5 A T C C G T G A C C T 3. Hy cho bit trnh t nucleotide trn mRNA c m ha t gen ny? A. 3 U A G G C A C U G G A 5 B. 3 A U C C G T G A C C U 5 C.5 U A G G C A C U G G A 3 D.5 A U C C G U G A C C U 3 24. Tuesday, October 08, 2013 24 Cu hi cng c Cu 5: Trt t nucleotide hp Pribnow? A. 5 T A T A T A 3 B. 3 T A T A T A 5 C.5 T A T A A T 3 D.3 T A T A A T 5 25. Tuesday, October 08, 2013 25 Ti liu tham kho 1. Phm Thnh H (2010), Di truyn hc, NXB Gio dc Vit Nam. 2. L Duy Thnh, T Ton, L Thng v inh on Long (2007), Di truyn hc, NXB Khoa hc v k thut, H Ni. 3. H Hunh Thy Dng (2008), Sinh hc phn t, NXB Gio dc. 4. Cng vi t liu, hnh nh v clip tham kho trn mng Internet. 26. Tuesday, October 08, 2013 26 Cm n c v cc bn ch lng nghe!