Production of food grade yeasts

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<ul><li><p>ISSN 1330-9862 review</p><p>(FTB-1659)</p><p>Production of Food Grade Yeasts</p><p>Argyro Bekatorou*, Costas Psarianos and Athanasios A. Koutinas</p><p>Food Biotechnology Group, Department of Chemistry, University of Patras, GR-26500 Patras, Greece</p><p>Received: January 30, 2006Accepted: March 20, 2006</p><p>Summary</p><p>Yeasts have been known to humans for thousands of years as they have been used intraditional fermentation processes like wine, beer and bread making. Today, yeasts are alsoused as alternative sources of high nutritional value proteins, enzymes and vitamins, andhave numerous applications in the health food industry as food additives, conditionersand flavouring agents, for the production of microbiology media and extracts, as well aslivestock feeds. Modern scientific advances allow the isolation, construction and industrialproduction of new yeast strains to satisfy the specific demands of the food industry. Typesof commercial food grade yeasts, industrial production processes and raw materials arehighlighted. Aspects of yeast metabolism, with respect to carbohydrate utilization, nutri-tional aspects and recent research advances are also discussed.</p><p>Key words: food grade yeasts, single cell proteins (SCP), raw materials, propagation, bakersyeast, brewers yeast, distillers yeast, Torula, whey, kefir, probiotics</p><p>Introduction</p><p>Yeasts are a group of unicellular microorganisms mostof which belong to the fungi division of Ascomycota andFungi imperfecti. Yeasts have been known to humans forthousands of years as they have been used in fermenta-tion processes like the production of alcoholic beveragesand bread leavening. The industrial production and com-mercial use of yeasts started at the end of the 19th cen-tury after their identification and isolation by Pasteur. To-day, the scientific knowledge and technology allow theisolation, construction and industrial production of yeaststrains with specific properties to satisfy the demands ofthe baking and fermentation industry (beer, wine, ciderand distillates). Food grade yeasts are also used as sourcesof high nutritional value proteins, enzymes and vitamins,with applications in the health food industry as nutri-tional supplements, as food additives, conditioners andflavouring agents, for the production of microbiology me-dia, as well as livestock feeds. Yeasts are included in start-er cultures, for the production of specific types of fer-mented foods like cheese, bread, sourdoughs, fermentedmeat and vegetable products, vinegar, etc.</p><p>The significance of yeasts in food technology as wellas in human nutrition, as alternative sources of proteinto cover the demands in a world of low agricultural pro-duction and rapidly increasing population, makes theproduction of food grade yeasts extremely important. Alarge part of the earths population is malnourished, dueto poverty and inadequate distribution of food. Scien-tists are concerned whether the food supply can keep upwith the pace of the world population increase, withthe increasing demands for energy, the ratio of land arearequired for global food supply or production of bio-energy, the availability of raw materials, as well as themaintenance of wild biodiversity (14). Therefore, the pro-duction of microbial biomass for food consumption is amain concern for the industry and the scientific commu-nity.</p><p>The impressive advantages of microorganisms forsingle cell protein (SCP) production compared with con-ventional sources of protein (soybeans or meat) are wellknown. Microorganisms have high protein content andshort growth times, leading to rapid biomass production,which can be continuous and is independent from theenvironmental conditions. The use of fungi, especially</p><p>407A. BEKATOROU et al.: Food Grade Yeasts, Food Technol. Biotechnol. 44 (3) 407415 (2006)</p><p>*Corresponding author; Phone: ++30 2610 997 123; Fax: ++30 2610 997 105; E-mail: ampe@chemistry.upatras.gr</p></li><li><p>yeasts, for SCP production is more convenient, as theycan be easily propagated using cheap raw materials andeasily harvested due to their bigger cell sizes and floc-culation abilities. Moreover, they contain lower amountsof nucleic acids than bacteria (57).</p><p>Yeast Metabolism</p><p>Yeasts are facultative anaerobes, and can grow withor without oxygen. In the presence of oxygen, they con-vert sugars to CO2, energy and biomass. In anaerobic con-ditions, as in alcoholic fermentation, yeasts do not growefficiently, and sugars are converted to intermediate by--products such as ethanol, glycerol and CO2. Therefore,in yeast propagation, the supply of air is necessary foroptimum biomass production. The main carbon andenergy source for most yeasts is glucose, which is con-verted via the glycolytic pathway to pyruvate and by theKrebs cycle to anabolites and energy in the form of ATP.Yeasts are classified according to their modes of furtherenergy production from pyruvate: respiration and fermen-tation. These processes are regulated by environmentalfactors, mainly glucose and oxygen concentrations. In res-piration, pyruvate is decarboxylated in the mitochond-rion to acetyl-CoA which is completely oxidized in thecitric acid cycle to CO2, energy and intermediates to pro-mote yeast growth. In anaerobic conditions, glucose isslowly utilized to produce the energy required just tokeep the yeast cell alive. This process is called fermen-tation, in which the sugars are not completely oxidizedto CO2 and ethanol. When the yeast cell is exposed tohigh glucose concentrations, catabolite repression occurs,during which gene expression and synthesis of respira-tory enzymes are repressed, and fermentation prevailsover respiration. In industrial practice, catabolite repres-sion by glucose and sucrose, also known as Crabtree ef-fect, may lead to several problems, such as incompletefermentation, development of off-flavours and undesirableby-products as well as loss of biomass yield and yeastvitality (810).</p><p>Yeasts can metabolize various carbon substrates butmainly utilize sugars such as glucose, sucrose and malt-ose. Sucrose is metabolized after hydrolysis into glucoseand fructose by the extracellular enzyme invertase. Mal-tose is transferred in the cell by maltose permease, andmetabolized after hydrolysis into two molecules of glu-cose by maltase. Some yeasts can utilize a number ofunconventional carbon sources, such as biopolymers,pentoses, alcohols, polyols, hydrocarbons, fatty acids andorganic acids, which is of particular interest to food andenvironmental biotechnologists. For example, lactose can</p><p>be utilized by yeasts that have the enzyme b-galactosi-dase. The yeasts of genera Kluyveromyces and Candida cangrow e.g. in whey, yielding biomass under certain condi-tions, with applications in food production. Biopolymerslike starch, cellulose, hemicellulose and pectin can bemetabolized by some yeasts directly, or after hydrolysisby non-yeast enzymes. Some yeast species of Hansenula,Pichia, Candida and Torulopsis are also able to grow onmethanol as sole energy and carbon source. The inabil-ity of yeasts to ferment certain sugars can be overcomeby r-DNA technology, introducing genes of the corre-sponding enzymes from other species (8,11). Finally, ele-</p><p>ments like N, P, S, Fe, Cu, Zn and Mn are essential to allyeasts and are usually added to the growth media. Mostyeasts are capable of assimilating directly ammoniumions and urea, while very few species have the ability toutilize nitrates as nitrogen source. Phosphorus and sul-phur are usually assimilated in the form of inorganicphosphates and sulphates, respectively.</p><p>Food Grade Yeasts</p><p>Various microorganisms are used for human con-sumption worldwide as SCP or as components of tradi-tional food starters, including algae (Spirulina, Chlorella,Laminaria, Rhodymenia, etc.), bacteria (Lactobacillus, Cellulo-monas, Alcaligenes, etc.), fungi (Aspergillus, Penicillium, etc.)and yeasts (Saccharomyces, Candida, Kluyveromyces, Pichiaand Torulopsis) (6,7). Among the yeast species, Saccharo-myces cerevisiae and Candida utilis are fully accepted forhuman consumption, but very few species of yeast arecommercially available.</p><p>The most common food grade yeast is Saccharomycescerevisiae, also known as bakers yeast, which is usedworldwide for the production of bread and baking pro-ducts. S. cerevisiae is the most widely used yeast species,whose selected strains are used in breweries, wineriesand distilleries for the production of beer, wine, distil-lates and ethanol. Bakers yeast is produced utilizing mo-lasses from sugar industry by-products as a raw mate-rial. Commercial S. cerevisiae and other yeast productsavailable to cover the needs of the baking and alcoholicfermentation industries or for use as nutritional supple-ments for humans and/or animals are described in thefollowing paragraphs.</p><p>Bakers yeast</p><p>Fresh bakers yeast consists of approximately 3033 %of dry materials, 6.59.3 % of nitrogen, 40.658.0 % ofproteins, 35.045.0 % of carbohydrates, 4.06.0 % of lipids,5.07.5 % of minerals and various amounts of vitamins,depending on its type and growth conditions. Commer-cial fresh bakers yeast includes products in liquid, creamyor compressed forms and active dry yeast. Compressedbakers yeast is the most commonly used product, con-sisting of only one yeast species, S. cerevisiae. Specialstrains of S. cerevisiae can be used for the production ofdry yeast products, like active dry yeast or instant dryyeast. Active dry yeast consists of grains or beads of livedried yeast cells with leavening power, while instantdry yeast usually comes in the form of fine particles thatdo not require rehydration before use. Unlike active dryyeast, inactive dry yeast is a product without leaveningproperties, used for the conditioning of dough proper-ties in baking or the development of characteristic fla-vour.</p><p>Brewers yeasts</p><p>Pure brewers yeast cultures are produced industrial-ly to supply the brewing industry. Usually two Saccharo-myces species are used: S. uvarum, formerly known as S.carlsbergensis, which is used for the production of severaltypes of beer with bottom fermentation (lager yeasts),and S. cerevisiae, which conducts top fermentation (ale</p><p>408 A. BEKATOROU et al.: Food Grade Yeasts, Food Technol. Biotechnol. 44 (3) 407415 (2006)</p></li><li><p>yeasts). Due to recent reclassification both ale and lageryeast strains are considered S. cerevisiae species. Top-fer-menting yeasts are used for the production of ales, porters,stouts, wheat beers, etc., and bottom-fermenting yeastsare used for lager beers like Pilsners, Bocks, Americanmalt liquors, etc. (12). Inactive brewers yeast prepara-tions, made from inactive yeast and other special ingre-dients, are produced commercially to be used as nutrientsto reinitiate or avoid sluggish and stuck fermentations.</p><p>Nutritional brewers yeast</p><p>Commercial, nutritional brewers yeast is inactive yeast(dead yeast cells with no leavening power), remainingafter the brewing process. Brewers yeast is produced bycultivation of S. cerevisiae on malted barley, separatedafter the wort fermentation, debittered and dried. It isan excellent source of protein and it is used as a nutrientsupplement rich in B vitamins. Brewers yeast products areusually found in the form of powders, flakes or tablets,or in liquid form. Liquid yeast contains enzymaticallydigested yeast for better digestion, absorption and utiliza-tion. Brewers yeast should not be confused with bre-wers type yeasts, which are pure yeasts usually grownon enriched cane or beet molasses under controlled pro-duction conditions, and are not by-products of the brew-ing process.</p><p>Brewers yeast is an excellent source of B vitamins,Ca, P, K, Mg, Cu, Fe, Zn, Mn and Cr and has been stu-died extensively for its medicinal properties. It is oftenused for the treatment of diabetes (regulation of insulinlevels), loss of appetite, chronic acne, diarrhoea, etc. (1315). It is also recommended as a dietary supplement forhealthy hair and nails. Nevertheless, according to some,brewers yeast is suspected of causing various problems,like chronic fatigue, memory disorders, immunodefi-ciency, irritable bowel syndrome, allergies, etc., mainlydue to the presence of yeast antigens and high amountsof Cr and salicylates (16,17).</p><p>Wine yeasts</p><p>A wide variety of pure yeast cultures, mainly Sac-charomyces (S. cerevisiae, S. bayanus, S. uvarum, S. oviformis,S. carlsbergensis, S. chevalieri, S. diastaticus, S. fructuum, S.pasteurianus, S. sake, S. vini, etc.) are produced industrial-ly for the use in induced wine fermentations, accordingto the industrial demands for fermentation efficiency andproductivity. The suitable type of yeast is selected withrespect to the geographical area, climate, type of grapesand desirable organoleptic quality of the product (taste,aroma, colour, tannin and glycerol content, etc.). Pure yeastcultures are also used to conduct specific types of fer-mentations, like bottle fermentation of Champagne andsparkling wines, or to treat stuck and sluggish fermen-tations.</p><p>Distillers yeasts</p><p>Distillers yeasts are used for the industrial produc-tion of alcohol and spirits (brandy, whiskey, rum, tequila,etc.). They are usually isolated from industrial fermen-tations of fruit pulps and beet or sugar cane molasses.Their selection depends on the desired product proper-ties, including flavour, alcohol yield, productivity, and</p><p>other technological features. Generally, distillers yeastsmust exhibit low foam formation, high stress-toleranceand high alcohol yields. They must also form controlledamounts of ethyl esters, aldehydes, fatty acids and high-er alcohols, which is an important prerequisite for theproduction of fine quality distillation products. Distillersyeasts must be able to ferment various substrates, suchas corn, barley, wheat, potato, etc. after hydrolysation infermentable sugars. They must be able to conduct fastfermentations with high productivities and low produc-tion costs, and tolerate high temperatures, osmotic pres-sures and alcohol concentrations (1820).</p><p>Probiotic yeasts</p><p>Probiotics are live microbial feed supplements whichbeneficially affect the host animal by improving its in-testinal microbial balance, or by a wider definition pro-biotics are microbial cell preparations or components ofmicrobial cells that have a beneficial effect on the healthand well-being of the host (21). Probiotic properties ofyeasts, like S. cerevisiae, have been reported and display-ed as the ability to survive through the gastrointestinal(GI) tract and interact antagonistically with GI pathogenssuch as Esherichia coli, Shigella and Salmonella. Specifically,S. boulardii, a thermophylic, non-pathogenic yeast, has beenused for more than 50 years as a livestock feed probioticsupplement as well as therapeutic agent for the treat-ment of a variety of gut disorders like diarrhoea. Thisyeast is safe, it is resistant to antibiotics, achieves highcell numbers in the intestine in short time, does not per-manently colonize the intestine and is quickly clearedafter the cease of administration. Its probiotic effects...</p></li></ul>

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