probiotics: how comes they work · probiotic, but as well the prebiotic and its natural...
TRANSCRIPT
PROBIOTICS:
HOW COMES THEY WORK ?
Goals of this presentation
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- Overview on the latest knowledge on our microbiotas
- Review of few probiotic’s mechanism of action ( non exhaustive list)
Our knowledge is growing
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2012 HUMAN MICROBIOME PROJECT Structure function and diversity of the healthy
human microbiome; N AT U R E | VO L 4 8 6 | 1 4 J U N E 2 0 1 2
5 floras of our body
3 Enterotypes in our gut
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5 enterotypes in the vagina
Vaginal microbiome of
reproductive-age women
Jacques Ravela,1, Pawel
Gajera, Zaid Abdob, G. Maria
Schneiderc, Sara S. K.
Koeniga, Stacey L. McCullea,
et al. Edited by Jeffrey I.
Gordon, Washington
University School of Medicine,
St. Louis, MO, and approved
May 7, 2010 (received for
review March 14, 2010)
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5 Enterotypes in the vagina
Vaginal microbiome of reproductive-age women
Jacques Ravela,1, Pawel Gajera, Zaid Abdob, G. Maria Schneiderc, Sara S. K. Koeniga, Stacey
L. McCullea, et al. Edited by Jeffrey I. Gordon, Washington University School of Medicine, St.
Louis, MO, and approved May 7, 2010 (received for review March 14, 2010)
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All of us similar… but different
Conclusion: “Human microbiology is a complex world”
Few figures…
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25,000 genes 3000 genes
Few figures…
3.300.000 genes
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10,000 billion cells 1,000 billion cells 1 billion cells
Few figures…
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PROBIOTICS:
HOW COMES THEY WORK ?
Development of a
probiotic product
Selection of the best candidate…
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Screening Screening method Intestinal probiotic Vaginal probiotic
Pathogens inhibition X X
Gastric resistance X
Ph 4 resistance x
Bilious resistance X
Adhesion capacity x x
Adhesion inhibition x x
Lactic acid and H202 production
X
Industrial feasability
Human clinicals
The production process…
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Since 1991 (Elo et al., Lett. Appl. Microbiol.) Differences of adhesion of the strain L. rhamnosus GG depending on its product’s origin
2010 (Grześkowiak et al., British J. Nutr.) Inhibitory capacities on the pathogens cellular adhesion of L. GG are significantly different depending on its origin
the manufacturing process influence the characteristics of the strain
impact on clinical trials
2012 (Sảnchez et al., Trends in Food Science & Technology) The development of a dietary probiotic should integrate the influence of the technical constraints.
Bacterial training
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Unlike other probiotic producers, when manufacturing our probiotic we use a unique process called 3Biotic formula. It allows to administrate in the final product not only the probiotic, but as well the prebiotic and its natural postbiotics, integrated in a synergetic compound. The 3Biotic formula is generating a unique efficacy by creating a “bacterial training” effect through the stimulation of specific genes of the probiotic strain. As a result, probiotics coming from 3Biotic formula have an increased efficacy and confirmed in a recently published study(1) outstanding ability to resist to gastric acid and bile, inhibit pathogens and adhere to intestinal wall for biofilm creation.
Increased resistance to gastric acid than native strain
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1,00E+00
1,00E+01
1,00E+02
1,00E+03
1,00E+04
1,00E+05
1,00E+06
1,00E+07
1,00E+08
0 45 min 90 min
UF
C/
ml
Lcr35® Lcr® sachet Lcr® gélule Lcr® Lénio
** ** **∆ ** ** **∆
Resistance to gastric acid : CFU evolution at Ph3
*(1) In vitro comparison of probiotic properties of Lactobacillus rhamnosus Lcr, influences of manufacturing process. / Nivoliez A. et al. / Journal of biotechnology 2012
Bacterial training
Protexin IBS
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-1,42 -1,74
-4,03
-7,87
-6,44
-7,72
-5,87
-6,94 -7,47 -7,42
-8,24 -8,04 -8,35
-7,95 -8,18 -9
-8
-7
-6
-5
-4
-3
-2
-1
0
EPEC ETEC Shigella flexnerfi
Without Lcr Bacilor (Lcr Restituo) Capsule Lcr35 Bacilor (Lcr Restituo) Packet Florea (Lcr Lenio)
(1) (Source : 149. Nivoliez A et al. Influence of manufacturing processes on in vitro properties of probiotic strain Lactobacillus
rhamnosus Lcr35®. Journal of Biotechnology. 2012(in press).
Measure of logarithmic pathogens’ growth after 24h of co-incubation (means ± standard deviation)
Bacterial training
Protexin IBS
0%
10%
20%
30%
40%
50%
60%
70%
80%
Glucose Glycogen
% o
f su
gar
con
sup
tio
n
Lcr35® Lcr Regenerans®
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Bacteria coming from 3biotic formula has an increased
capacity to use Glycogen to produce lactic acid
Usage of glycogen as a substrate
Bacterial training
( Gynophilus)
1,E+00
1,E+01
1,E+02
1,E+03
1,E+04
1,E+05
1,E+06
1,E+07
1,E+08
1,E+09
T0 T4 T24 T28
CF
U / m
l
Temps (h)
vs Lcr35®
vs Lcr Lenio®
vs Lcr Regenerans®
Candida albicansseul
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Inhibition power on Candida albicans
=> Bacteria coming from 3biotic formula totally inhibit
C.albicans in 24h00, when native bacteria does not
Bacterial training
Protexin IBS
( Gynophilus)
Conclusion
The activity of a probiotic is
Strain dependent
Production process dependent
Lcr35
Lcr Regenerans
(Gynophilus)
The secret of probiotics:
interaction
Mechanisms of Action of Probiotics
With our mucosas
With our natural
flora
With our immune system
With our brain
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THEY
INTERACT
Conclusion
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Conclusion
Candiflor1: Clinical study with 409 women. The usage of Gynophilus during 3 weeks gave a 40% reduction rate of candidiasis recurrence on a 6 months observational period.
Dapoigny et al: intake of Lcr35 in IBS-D patients implicated a 36% reduction of Francis score, with a remanence of at least 15 days…
Mechanism of action of probiotics is an extremely complex subject. The more we know… the less we know.
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Conclusion
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Conclusion