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     Biofoc ProductionSystems orAquaculture

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     Composition and nutritional value of bioflocs

     Bioflocs are aggregates (flocs) of algae, bacteria,

     protozoans, and other kinds of particulate organic

    matter such as feces and uneaten feed.The biofloc community also includes animals that are

    grazers of flocs, such as some zooplankton and

    nematodes. Large bioflocs can be seen with the naked

    eye, but most are microscopic. Flocs in a typicalgreenwater biofloc system are rather large, around !

    to "!! microns, and will settle easily in calm water .

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    The nutritional #uality of biofloc to cultured animalsis good but rather $ariable. The dry%weight protein

    content of biofloc ranges from " to ! percent, with

    most estimates between &! and ' percent. Fat content

    ranges from !. to percent, with most estimates between and percent.

    ried bioflocs ha$e been proposed as an ingredient to

    replace fishmeal or soybean meal in a#uafeeds. The

    nutritional #uality of dried bioflocs is good, and trials

    with shrimp fed diets containing up to &! percent dried

     bioflocs show promise

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    Suitable culture species* basic factor in designing a biofloc system is the species to

     be cultured. Biofloc systems work best with species that are able

    to deri$e some nutritional benefit from the direct consumption of

    floc. Biofloc systems are also most suitable for species that can

    tolerate high solids concentration in water and are generally

    tolerant of poor water #uality. +pecies such as shrimp and tilapiaha$e physiological adaptations that allow them to consume

     biofloc and digest microbial protein, thereby taking ad$antage of

     biofloc as a food resource. early all biofloc systems are used to

    grow shrimp, tilapia, or carps. Channel catfsh and hybridstriped bass are examples o fsh that are not goodcandidates or biooc systems because they do nottolerate water with very high solids concentrationsand do not have adaptations to flter solids rom

    water.

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     systems

    Few types of biofloc systems ha$e been used in

    commercial a#uaculture or e$aluated in research. Biofloc

    systems e-posed to natural light include outdoor, a

    comple- mi-ture of algal and bacterial processes control

    water #uality in such greenwater/ biofloc systems. 0ost

     biofloc systems in commercial use are greenwater.

    These systems are operated as brown% water/ biofloc

    systems, where only bacterial processes control water

    #uality.The specifications and performance of $arious biofloc

     production systems are discussed in more detail at the end

    of this publication.

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    Denitrification and sludge treatment

    *lkalinity can be reco$ered in denitrification units. itrateaccumulates in most intensi$e biofloc systems because of

    ongoing nitrification. 1f unchecked, nitrate concentration

    reflects the cumulati$e feed loading to the system. itrate

    accumulation can be tempered by dilution through water

    e-change, but this defeats the purpose of intensi$e water use

    and reduces biosecurity.

    enitrification units are used as part of a water conser$ation

    and biosecurity strategy where it is also a cost issue to

    conser$e salts. This is an acute need in superintensi$esaltwater systems for shrimp, especially those located inland.

    Furthermore, the discharge of saline effluent is restricted or

    regulated in many areas, especially inland.

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    enitrification units are operated under generally

    #uiescent and ano-ic conditions. +olids can beshunted to a side%stream tank and allowed to

    accumulate. * low flow of culture water, sufficient to

     pro$ide a detention time of to " days, is ade#uate to

    control nitrate concentration. +olids accumulation will

    reach a steady state.

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    Problems+uspended solids are central to the function of biofloc systems.

    The capacity to control solids concentration depends on system

    configuration. 2-cessi$e solids concentration is counter%

     producti$e because solids can log gills of fish or shrimp.

    1t also increases the energy re#uired for mi-ing to keep solids in

    suspension and aeration to meet the o-ygen demand of ele$atedwater respiration. 2-cessi$e solids concentration also means that

    the response time in the e$ent of system failure is $ery short,

    often less than hour. 3ccasionally and unpredictably bioflocs

    will de$elop that include large numbers of filamentous bacteria.This so%called filamentous bulking/ effect makes flocs slow to

    settle and makes it difficult to control solids concentration.

    Filamentous bacteria can also clog shrimp gills and cause

    mortality.

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    *s in most recirculating a#uaculture systems,

    nutrients and minerals (especially metals) accumulatein the water of intensi$ely managed biofloc systems.

    1n shrimp raceways with low water e-change rates,

    nitrate can accumulate to se$eral hundred mg4L, a

    le$el that reduces shrimp feed consumption. 1ncludingthe capacity for denitrification in intensi$ely managed

     biofloc systems is recommended. 1n marine systems,

    maintaining a nitrate concentration of about ! mg4L

    is an effecti$e way to minimize the production ofhighly to-ic hydrogen sulfide.