oz biosciences dreamfect gold reagent
DESCRIPTION
transfection and protein expressionTRANSCRIPT
DreamFectTM Gold
The art of delivery systems
Examples of cells successfully tested
Cell Lines Cell Types Species
293, 293T
3T6
A172
A549
B16-F10
BEAS-2B
BHK-21
CHO, CHO-K1
COS-1, COS-7
CV-1
H441
HaCaT
HEK293
HeLa, HeLa-S3
HepG2
Jurkat
K562
L929
M1
MCF-7
MDCK
N2A
NIH-3T3
PC-12
SH-SY5Y
SW480
U87
Vero
Primary hippocampal neurons
Primary Glioma cells
Kidney
Embryonic Fibroblast
Glioma
Non-small cell lung carcinoma
Melanoma
Bronchial epithelial
Kidney
Ovary (epithelial-like)
Kidney
Kidney (fibroblast-like)
Lung epithelial
Keratinocyte
Kidney
Cervix carcinoma
Hepatoma
Acute T lymphocyte
Myelogenous leukemia
Fibrosarcoma
Kidney epithelial
Breast adenocarcinoma
Kidney
Neuroblastoma
Fibroblast
Adrenal pheochromocytoma
Neuroblastoma
Colon adenocarcinoma
Glioma
Kidney
Human
Mouse
Human
Human
Mouse
Human
Hamster
Chinese Hamster
Green Monkey
Monkey
Human
Human
Human
Human
Human
Human
Human
Mouse
Mouse
Human
Dog
Mouse
Mouse
Rat
Human
Human
Human
Green Monkey
Rat
Human
Please visit www.ozbiosciences.com
for a complete updated list of cells successfully
transfected with
DreamFect™ Gold
Catalogue Numbers
DG80500
DG81000
DG85000
DreamFect™ Gold 0.5 mL
DreamFect™ Gold 1 mL
DreamFect™ Gold 5 x 1 mL
Up to 500 transfections
Up to 1000 transfections
Up to 5000 transfections
DreamFect™, Lullaby® -siRNA transfection reagent andMagnetofection™ reagents (PolyMag, CombiMag, SilenceMag ViroMag, ViroMag R/L and FluoMag)are examples of innovations from OZ Biosciences.
Contact us to learn more about these products.
Optimized protocols for specific cell types and / or applications are
available upon request.
Technical Support: [email protected]
Customer Service:[email protected]
Sales department: [email protected]
DreamA treasure for transfection and protein expression
Parc Scientifique de Luminy - BP 13 - Case 922 - Marseille 13288 Cedex 9 - France tel: +33 (0) 486 948 516 - fax: +33 (0) 486 948 515
w w w . o z b i o s c i e n c e s . c o m
Fig. 1: Several cell lines (1x105 cells/ well) were transfected with 1 µg of pEGFP plasmid DNA per well in a 24-well plate. Transfections were performed with 4 µL per well of DreamFect Gold transfection reagent. Percentage of transfected cells were measured 24h post transfection by FACS.
Based on an innovative technology, this new lipid-based reagent achieves extremely efficient DNA delivery into cells. DreamFect™ Gold protects DNA from degradation, forms very small complexes easily internalized by cells and promotes nucleic acid delivery in all cells. DreamFect™ Gold allows high reliable and reproducible transfection efficiency in multiple conditions: cells, amount of DNA, culture conditions, etc.
DreamFect™ Gold has an exceptional capacity to destabilize cell membranes, leading to the delivery of important nucleic acid amounts into the cytosol. Thanks to an improved cytoplasmic release process and a complete biodegradability, DreamFect™ Gold allows achieving very high level of transgene (protein) expression. Consequently, DreamFect™ Gold is the ideal reagent for protein production, biochemistry studies, cellular biology assays, viral production, western blot analysis, immunocytochemistry, microscopy, etc.
Greater protein expression level
DreamFect™ Gold has been successfully tested on a variety of cells, reaching up to 95-99 % transfection efficiency combined with high protein expression level.
Please see next page for a list of tested cell types or visit www.ozbiosciences.com for an updated and complete list.
Great for all cell types
DreamFect™ Gold is antibiotics- and serum-compatible and works over a broad range of cell confluence (between 20 to 90%).
Flexible and adapted to all culture conditions
This reagent is adapted to all types of nucleic acids (DNA, siRNA, mRNA, shRNA etc.) and all transfection applications:- Co-transfection & reverse transfection- Sequential, multiple & repetitive transfections- Production of pseudo-virusDreamFect™ Gold is fully suitable to High-Throughput Screening.
Versatile and multipurpose
Have a golden nugget in your lab!Try DreamFect™ Gold and you will surely adopt it!
The ideal reagent for all transfections
DreamFect™ Gold can be used with all types of nucleic acids and allows their delivery in a wide variety
of cell lines and primary cells. Benefit from state-of-the art in transfection and achieve high transfection
efficiency combined with superior transgene expression level.
DreamFect™ Gold is the latest transfection reagent based on the TEE-technology (Triggered Endosomal
Escape) which combines and exploits the properties of cationic lipids and polymers to achieve an extremely
efficient DNA delivery into cells. Because the level of transgene expression matters as well as the transfection
efficiency, OZ Biosciences has developed DreamFect™ Gold, the next powerful generation of lipopolyamines
with improved cytoplasmic release process and complete biodegradability.
DreamFect™ Gold, a treasure for transfection and protein expression Fig. 4: Cell lines were transfected with 1 µg of pEGFP plasmid DNA and 4 µL of DreamFect Gold reagent per well in a 24-well plate. The other transfection reagents were assayed according to the m a n u f a c t u r e r ' s instruction. The average intensity of GFP was measured in each well by FACS.
Fig. 5: Several cell lines were transfected with 0.5 µg of pLacZ plasmid DNA and 2 µL of DreamFect Gold in a 96-well plate. The other transfection reagents were assayed according to the manufacturer's instruction. Expression of β-galactosidase was measured 24 h post-transfection using the ONPG β-gal assay kit (catalog # G010001).
Fig. 7: HeLa cells were transfected with 0.5 µg of pLacZ plasmid and 2 µL of each reagent per well in a 96-well plate. The total amount of protein was monitored 24h post-transfection using the Bradford Protein Assay kit (catalog # BA00100).
Fig. 6: Hard-to-transfect Jurkat (suspension T cells) and primary rat hippocampal neurons s u c c e s s f u l l y transfected with pEGFP plasmid DNA and DreamFect Gold.
High and reproducible transfection efficiency
Fig. 3: Several cell lines (1x105 cells/ well) were transfected with 1 µg of pEGFP or pLacZ plasmid DNA and 4 µL of DreamFect Gold reagent per well in a 24-well plate. EGFP and β-Galactosidase expression were monitored 24 h post-transfection by fluorescence microscopy and X-Gal staining kit (catalog # GX-10003), respectively.
As a ready-to-use and simple reagent, experiment is quickly handled in a 3-steps procedure. Detailed protocols have been developed as well as optimal cell specific applications.
Rapid and straightforward procedure
�� Highly efficient
�� Achieve superior transgene expression level
�� Biodegradable - avoid undesired secondary effects
�� Applicable to a broad range of cells
DreamFect™ Gold features
�� Outperform other transfection reagents
�� Versatile and multipurpose
�� Suitable for a large spectrum of cell culture conditions
�� Rapid and straightforward procedure
DreamFect™ Gold formulation is composed by fully biodegradable lipids. The cationic moiety has been specifically designed in order to be quickly degraded into secondary products completely safe for cells. Due to this innovative and critical feature, high cell viability is maintained in every experiments.
Biodegradable - Non toxic
Please visit www.ozbiosciences.com for more results
90 %
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Fig. 2: Various cell lines (15 x 103 cell / well) were transfected with 0.25 µg (common cell lines) or 0.5 µg (hard-to-transfect) of pLacZ plasmid and 1 or 2 µL of DreamFect Gold reagent per well in a 96-well plate, respectively. β-Galactosidase expression was monitored 24 h after transfection using ONPG β-galactosidase assay kit (catalog # G010001).
Common cell lines Hard-to-transfect cell lines
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DreamFect™ Gold surpasses all competitors' products for transfection efficiency, transgene expression level and non toxicity.
Outperform all other transfection reagents tested
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No Toxicity
Cells
DNA + DreamFect Gold complexes
incubate 15 to 20 minutes
Assay (24/ 72 h)
DreamFect TM Gold(4 µL per µg DNA)
DNA, siRNA or ODN(0.25 to 20 µg)
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CHO-K1� � HeLa-S3� � BEAS-2B� HEK293� � BHK-21�� MDCK
Jurkat� � Primary Neurons