oxi proteomics
TRANSCRIPT
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Aging, UV Irradiation, Pollution Inflict damage to our proteins
An#AgeTreatment
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Aging, UV Irradiation, Pollution Inflict damage to our proteins
An#AgeTreatment
An#-AgingPerformance
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Patents: (EP11305772.3 - PCT/EP2012/061749 - WO2012175519)
Protein carbonyls Detection: OXIPROTEOMICS’ TECHNOLOGY
DIRECT DETECTION
+ Fluorophore
Protein
Protein
+ Fluorophore
Protein
NH2
+ Fluorophore
O Protein
Fluorophore
Detection and quantification of oxidized proteins
using Difference In Gel Electrophoresis: Oxi-DIGE
Protein labelling, automated separation, gel scanning and image analysis
Automated Protein separation, Quantification Analyses and Protein ID
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Our Offer: Carbonyl Score
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Reconstituted skin
Protein Identification
Mass Spectrometry
Efficacy Testing in Dermo Cosmetics
Carbonyl-Score
Oxi-Proteome
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Tape Stripping of human stratum corneum
Protein extraction Starting from 1.5 cm2 of tape stripping surface
Extracted proteins are sufficient for 1 analysis
4µg 8µg 16µg 4µg 8µg 16µg
Intrinsic Aging
Photo Aging
Photo Aging
Intrinsic Aging
Efficacy Testing in Dermo Cosmetics Carbonyl-Score
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Case Study Anti-Pollution Performance
Control COV COV + Actives
Dermal Fibroblasts
COV Volatile Organic
Compounds
COV + Actives
Carbonyl-Score
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Oxi-Proteome
ListofDifferenciallyCarbonylatedSpots(p<0,05) SpotIndex
NormalisedMeanValues Varia#onFactor
Anova (p)
Control COV COV+Ac#fs
952 0,803 2,093 0,809 2,6 0,01 653 1,369 1,720 1,336 1,3 0,01
1409 1,172 1,480 1,155 1,3 0,01 949 0,854 1,264 0,938 1,5 0,01 495 0,506 1,251 0,569 2,5 0,03
802 1,282 1,717 1,259 1,4 0,03
649 1,320 1,797 1,163 1,5 0,03
Case Study Anti-Pollution Performance
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Kit!(in house analyse)! Services!
Quantitative Performance! +! ++++!
Direct Detection! No! Yes!
Assay Variation! High! Minimal!
Optimized and !Validated protocols! No! Yes!
Detection limit! Fentomole! Fentomole!
Time to results! 30 days! 5 days!
Weekly Thrughput! 10 samples! 400 samples!
Competitive Advantages
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Quantitative read-out for : Target identification and molecular mechanisms of protection Quick turn-around of results : Cost Effective :
Final detailed report includes : study description, experimental procedure, results (raw data et HD images) , evaluation and statistical analysis, conclusions.
Scientific objectivation for research and marketing claims
Ø evaluating ingredients or finished products performance
Ø development of performant experimental models for efficacy testing
Ø 5 days Carbonyl score Ø 15 days Oxi-Proteome
Ø Price covers from sample preparation to data ready for communication
Value Proposition
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Bertrand Friguet Scientific Advisor
Martin Baraibar PDG
Claude Hennion Business Developer
Andrea Cavagnino Project Manager
Lucie Brasseur Communications Advisor
The Team
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