operating manual flat specimen system 2 for leica em pact2
TRANSCRIPT
Operating ManualFlat Specimen System 2for Leica EM PACT2
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Contents
page
1. Installation 8
1.1 Packing List 8
2. Operation 10
2.1 Pre-loading of the carrier 10
2.2 Filling 12
2.3 Loading of the pod 14
2.4 Mounting pod on bayonet loading device 17
3. Work routine for unloading 18
4. Maintenance of the pods 19
.
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@L%1L @F=%1<<'B="3=$#
?2BL ?1$2$B
Abbreviations and symbols used in this manual
M(?F"=3@=%1@JF"(3=1F@%JF"%=I$
'B$"L
Please read this instruction manual carefully
before working with the Flat Specimen Carrier
System.
7==$@=1F@N%=3O$%$P="3%23"$L
Q
Safety Instructions
Liquid Nitrogen (LN2)
RI$@%SF"O1@H%S1=I% <1K'1#% @1="FH$@% T0&,U
?<$3B$%)$3"%1@%(1@#%0&,%1B%$P="$($<A%2F<#L%M=
)F1<B%3=%:*V+%W8L%&1="FH$@%H3B%TX&,U%$B23?$B
3=%Y$"A%<FS%=$(?$"3='"$%J"F(%=I$%)F1<1@H%0&,L
ZF=I%0&,%3@#%X&
,%3B%S$<<%3B%2FF<$#%$<$($@=B
T$LHL% ?1?$BN% Y3<Y$BN% IFB$BN% 2F@=31@$"B% F"
B=F??$"BU%23@%23'B$%B$Y$"$%J"FB=%)1=$%3@#
)'"@B%=F%=I$%BO1@%3@#%$A$BL
RI$@%0&,%$Y3?F"3=$BN%1=%$P?3@#B%1@%3%"3=1F
FJ%*[\--L%*%<1="$%0&,%?"F#'2$B%3<(FB=%*%(.%FJ
X&,L%83"$%BIF'<#%=I$"$JF"$%)$%=3O$@%=F%$@B'"$
=I3=% SI$@% <3"H$% K'3@=1=1$B% FJ% @1="FH$@
$Y3?F"3=$%T$LHL%SI$@%="3@BJ$""1@H%0&,UN%=I$
"FF(%BIF'<#%3<S3AB%)$%S$<<%Y$@=1<3=$#L
]$(FY1@H% 0&,%S3B=$[% #'(?% 0&
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SI$"$%1=%S1<<%$Y3?F"3=$%"3?1#<A%3@#%B3J$<AL
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1@I3<$#%<1O$%31"L%X&,%1B%@F@:=FP12N%)'=%3%I1HI
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#13=$% J31@=1@H% 3@#% #$$?% '@2F@B21F'B@$BB
S1=IF'=%3@A%?"$Y1F'B%BA(?=F(BL
RI$@%=I$"$%1B%#F')=%3)F'=%=I$%3#$K'32A%FJ
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FPAH$@%('B=%@F=%#"F?%)$<FS%*/_L
MJ% 3@% '@2F@B21F'B% ?$"BF@% B=3AB% 1@% 3% <FS
FPAH$@%$@Y1"F@($@=%=I$@%#$3=I%(3A%F22'"L
MJ%)"$3=I1@H%B=F?BN%3??<A%3"=1J1213<%"$B?1"3=1F@
3=% F@2$%3@#%@F=1JA% #F2=F"% 3@#%3()'<3@2$
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9I$%$Y3?F"3=1F@%"3=$%J"F(%a$S3"%Y$BB$<B%23@
"1B$%=F%B$Y$"3<%<1="$B%3%#3A%1J%=I$A%3"$%#$J$2=1Y$
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FY$"%(3@A%A$3"B%FJ%'B$L
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<3A$"%1B%JF"($#%?"$Y$@=1@H%3%<3"H$%="3@BJ$"%FJ
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$Y3?F"3=$BL%GFS$Y$"N%F@2$%=I$%F)b$2=%I3B
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M@%=I$%23B$%FJ%)'"@B%J"F(%0&,%B?<3BI$BN%"1@B$
=I$%3JJ$2=$#%BO1@%1(($#13=$<A%S1=I%?<$@=A%FJ
S3=$"%3=%I3@#%=$(?$"3='"$L%;F"%B$"1F'B%)'"@B
3""3@H$%JF"%3%BO1@%B?$213<1B=%=F%B$$%=I$(%3=
F@2$L
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3JJ$2=$#%$A$%1(($#13=$<A%S1=I%S3=$"%3=%I3@#
=$(?$"3='"$%3@#%3""3@H$%JF"%3@%$A$%B?$213<:
1B=%=F%B$$%1=%3=%F@2$L
&$Y$"%'B$%H<3BB%a$S3"%Y$BB$<B% 1@%=I$%<3)
T$B?$213<<A%@F%H<3BB%a$S3"B% <3"H$"% =I3@%,
<1="$B% 23?321=AU% S1=IF'=% 2F(?<$=$%($=3<
$@Y$<F?$[%X<3BB%a$S3"B%FJ=$@%)'"B=%JF"%@F
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(1BI3@#<1@H% T$LHL% 2F@=32=% S1=I% ($=3<
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?"F=$2=1Y$%H<3BB$B%SI$@%'B1@H%0&,%1@%3%H<3BB
a$S3"L
D
Fig.1.1: When working with LN2 avoid protective glasses (a), boots (c), walking shoes (e) and protective
gloves (g) out of which the LN2 cannot easily escape if entered.LN
2 splashing into the closed protective glasses
(a), open boots (c), shoes (e) or protective gloves (g) evaporates suddenly and can cause serious burns.
Always use protective glasses (b) with side protection which are open at the top and at the bottom. Only use
boots if you have loose (not narrow) trousers coming outside the boots (d) and completely covering the gap.
Wear only open slip-on sandals (f) in the lab, no walking shoes or court shoes. Always wear cufless trousers
if you wear slip-on sandals.
Never wear protective gloves when pouring LN2 or when putting the Dewar head on the Dewar vessel. Just use
an open flannel cloth (h) to protect your hands from the cold. Gloves should be used only to grasp dry cold
parts. They are unsuitable for LN2 work.
+
!@<A% 'B$% ($=3<% a$S3"B% B?$21J123<<A
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B=F"3H$%FJ%0&,L%;F"%"F'=1@$%2"AF?"$?3"3=1F@
($=3<%="F'HIB%T*%2(%B=A"FJF3(%1@B'<3=1F@UN
B=A"FJF3(%2F@=31@$"B%F"%?<3B=12%="F'HIB%3"$
$(1@$@=<A%B'1=3)<$%3@#%$@B'"$%2F(?<$=$<A
"1BO%J"$$%2"AF?"$?3"3=1F@L
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=I$%(3@'J32='"$"L%9I$%$Y3?F"3=1F@%"3=$%FJ%3@
'@#3(3H$#%($=3<%a$S3"% BIF'<#% )$%S$<<
)$<FS%*%<1="$%FJ%0&,%?$"%#3AL%a$J$2=1Y$%a$S3"
Y$BB$<B%S1=I%I1HI$"%$Y3?F"3=1F@%"3=$B%3"$%3
B3J$=A%"1BON%3@#%BIF'<#%)$%=3O$@%F'=%FJ%SF"O
F"%"$?31"$#L
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SI$"$% 1=% 23@% $P2I3@H$% S1=I% =I$% "FF(
3=(FB?I$"$L%9I$%)F1<1@H%?F1@=%FJ%0&,%T:*V+
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T:*/.%W8UL%RI$@%=I$%$P2I3@H$%B'"J32$B%3"$
$P=$@B1Y$%$@F'HIN%FPAH$@%J"F(%=I$%31"%S1<<%)$
=3O$@%'?%1@%$P2I3@H$%JF"%@1="FH$@L%0&,%S1=I%3
I1HI%<1K'1#%FPAH$@%2F@=$@=%I3B%3%J31@=<A%)<'1BI
2F<F'"L%Concentrated liquid oxygen
promotes vigorous burning!
53O$%B'"$%=I3=%AF'"%a$S3"%Y$BB$<%1B%J1<<$#%F@<A
S1=I% 0&,L% 7??<A% 3% @F=$% 1@% =I$% 2$@="3<
#1B="1)'=1F@%?<32$%B=3=1@H%2<$3"<A
!&0d%0MefMa%&M9]!X4&
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#$<1Y$"$#% J"F(% =I$"$L%8I$2O% =I$% 2F<F'"% FJ
2"AFH$@[%Z<'1BI%2F<F'"%1@#123=$B%=I$%?"$B$@2$
FJ%3%I1HI%?$"2$@=3H$%FJ%<1K'1#%FPAH$@L
9I$%2F@2$@="3=1F@%FJ%<1K'1#%FPAH$@%1@2"$3B$B
#'"1@H%<F@H%?$"1F#B%FJ%B=F"3H$%3B%1=B%)F1<1@H
?F1@=%T:*/.%W8U%1B%I1HI$"%=I3@%=I$%)F1<1@H%?F1@=
T:*V+%W8U%FJ%0&,L
\
HAZARD WARNING
0MefMa%&M9]!X4&N%0&,
%%>'JJF23=1F@
: 7@A%Y$BB$<%2F@=31@1@H%0&,%1B%3%?F=$@=13<%I3E3"#
: !@$%<1="$%0&,%?"F#'2$B%\--%<1="$B%&
,%H3B
: &,%H3B%1B%F#F'"<$BB%3@#%=3B=$<$BB
: !PAH$@%<$Y$<B%23@%K'12O<A%#"F?%1@%2F@J1@$#%B?32$B%#'$%=F%#1B?<32$($@=%FJ
FPAH$@%)A%&,%SI$@%'B1@H%F"%#1B?$@B1@H%<3"H$%YF<'($B%FJ%0&
,
: 9I1B%23@%23'B$%1(($#13=$%J31@=1@H%3@#%'@2F@B21F'B@$BB
: Always use LN2 in well-ventilated areas
: 9"$3=%1=%S1=I%"$B?$2=g
%%>=F"3H$
: ;F"%"$3BF@B%($@=1F@$#%3)FY$%#F%@F=%B=F"$%J'<<%0&,%a$S3"B%1@%2F@J1@$#%B?32$B
%%Z'"@B
: 0&,%)F1<B%3=%:*V+W8L%M=%1B%$P="$($<A%2F<#%3@#%23@%23'B$%B$"1F'B%)'"@BL%6<$3B$
"$3#%=I$%B3J$=A%1@B="'2=1F@B ?"FY1#$#%S1=I%3<<%0$123%?"F#'2=B%JF"%=I$%2F""$2=%I3@#<1@H%FJ
<1K'1#%@1="FH$@g
/
1 Installation
1.1 Packing List
Flat Specimen System 2
Flat Specimen loading stationN%=I$%?"$:
<F3#1@H%"$2$BB%1B%Y1B1)<$%3=%=I$%"1HI=%I3@#
B1#$
100 Flat specimen carriersN%HF<#%?<3=$#
-LD((%=I12ON%*L,%((%1@%#13($=$"%C%,--!(
#$$?
1 Transfer slider
2 Loading deviceB%JF"%J<3=%B?$21($@%?F#L
Specimen punchN%*((%#13($=$"L
53@'3<%)3AF@$=%<F3#1@H%#$Y12$%T,%?2BU
Z3AF@$=%?F#%TD%?2BU
>3(?<$%?'@2I%JF"%B?$21($@%#1B2B%FJ%*((
#13($=$"
0F3#1@H%B=3=1F@%S1=I%B<1#$"
;<3=%B?$21($@%23""1$"%-LD((%=I12ON%*L,%((
1@%#13($=$"%C%,--!(%#$$?N%HF<#%?<3=$#
T*--%?2BLU
8"AF%="3@BJ$"%)FP%JF"%B?$21($@%23""1$"
9F"K'$%S"$@2I%S1=I%)1=
]'))$"%B3(?<$%?'@2I%B'??F"=%T@L%1LU
;1@$%JF"2$?B%S1=I%B="31HI=%=1?%T@L%1LU
;F"2$?B%S1=I%1@B'<3=1F@%2F3=1@H%T@L%1LU
8"AF:(3@1?'<3=F"%S1=I%5Q%=I"$3#%T@L%1LU
83B$%JF"%=FF<B%T@L%1LU
5 bayonet pods
V
The wrench is factory
adjusted to 30Ncm. Do not
change this value.
Torque wrench T3 UN%S1=I%,((%7<<$@%O$A
1@B$"=%T)UN% JF"%=1HI=$@1@H%%=I$%23""1$"%1@%=I$
?F#L%6'BI%=I$%,((%3<<$@%O$A%1@B$"=%1@=F%=I$
=F"K'$%S"$@2I%'@=1<%=I$%B?"1@H%IF<#$"
2<3(?B%1=L
a
b
Cryo transfer box JF"%%;<3=%>?$2%23""1$"L R$%"$2F(($@#%=F%'B$%=I$
;<3=%>?$21($@%>AB=$(%,%1@
2F()1@3=1F@%S1=I%=I$
!?=123<%RF"OB=3=1F@L
Optical Workstation.
*-
2.1 Pre-loading of the Flat
Specimen carrier
!?$@%?F#L
0F3#%)3AF@$=%?F#%1@=F%<F3#1@H%B=3=1F@%S1=I
=I$%)3AF@$=%J321@H%'?N%3B%BIFS@%1@%J1HL%,L*
Fig. 2.2:
2. Operation
6<32$%3%J<3=%B?$21($@%23""1$"%'?B1#$%#FS@
F@=F%3%J<3=%B'"J32$%3B%BIFS@%1@%J1HL%,L,L
Fig. 2.1: Bayonet pod in loading station.
Fig. 2.2: Flat specimen carrier upside down on a
flat surface
Fig. 2.3: Pick up of the carrier from
pre-loading recess with transfer slider.
7<=$"@3=1Y$<AN%AF'%23@%3<BF%)"1@H%=I$%?"$:
<F3#1@H%"$2$BB%FJ%=I$%<F3#1@H%B=3=1F@%1@=F
J1$<#%FJ%Y1$S%3B%BISF@%1@%J1HL%,L.L
6<32$%23""1$"%1@=F%?"$:<F3#1@H%"$2$BB
'?B1#$%#FS@N%3B%BIFS@%1@%J1HL%,L.L
**
612O%'?%23""1$"%S1=I%%="3@BJ$"%B<1#$"%%3B
BIFS@%1@%J1HBL%,L.L%9I$%23""1$"%1B%=I$@
<F23=$#%1@%=I$%(1<<$#%F'=%?3"=%FJ%=I$%="3@BJ$"
B<1#$"%=1?%TJ1HL%,LQLU
01J=%'?%23""1$"%1@%=I$%="3@BJ$"%B<1#$"%3@#%='"@
FY$"%3B%BIFS%1@%J1HL%,LD%3@#%,L+L
Fig. 2.6: Carrier ready for filling.
Fig. 2.5: Turning transfer slider over.
Fig. 2.4: Carrier in the milled out part of the
transfer slider.
*,
6'=%=I$%="3@BJ$"%B<1#$"%1@%=I$%hJ1<<1@H
?FB1=1F@iN%9I$%=1?%S1=I%=I$%23""1$"%1B%@FS
$3B1<A%%322$BB1)<$%TJ1HL%,L\UL
Fig. 2.8: Carrier filled with specimen and filling
medium.
6'BI%=I$%="3@BJ$"%B<1#$"%%S1=I%=I$%J1<<$#
23""1$"%1@=F%=I$%h<F3#1@H%?FB1=1F@i%3B
1@#123=$#%)A%=I$%3""FS%1@%J1HL%,LVL
;1<<%23""1$"%S1=I%B?$21($@%3@#%3??"F?"13=$
J1<<1@H%($#1'(%T$LHL%*:I$P3#$2$@$U%3B
BIFS@%1@%J1HL%,L/L
Fig. 2.7: Tip of the transfer slider in filling position.
Fig. 2.9: Pushing transfer slider in loading posrion.
2.2 Filling
*.
Fig. 2.10: Carrier in loading position.
Fig. 2.11: Transfer slider at end stop.
!@2$%=I$%="3@BJ$"%B<1#$"%S1=I%23""1$"%1B%1@
=I$%h<F3#1@H%?FB1=1F@i%TJ1HL%,L*-U%%1=%I3B
"$32I$#%=I$%$@#%B=F?%TJ1HL%,L**UL
*Q
01J=%?F#%3@#%="3@BJ$"%B<1#$"%F'=%FJ%<F3#1@H
B=3=1F@%=FH$=I$"N%3B%BIFS@%1@%J1HL%,L*QL
fB$%=F"K'$%S"$@2I%=F%<F2O%23""1$"%1@%?F#N
3B%BIFS@%1@%%J1HL%,L*.L
Fig. 2.13:Using torque wrench to lock pod.
Fig. 2.14: Lifting out pod.
53O$%B'"$%=I$%J32$%FJ%=I$%#13(F@#%FJ%=I$
J<3=%B?$21($@%?F#%1B%2F""$2=<A%3<1H@$#
S1=I%=I$%B'"J32$%?3"3<<$<%=F%=I$%23""1$"
TJ1HL%,L*,UL
2.3 Loading of the flat
specimen pod
Fig. 2.12: Diamond correctly aligned
with the surface parallel to the carrier .
The wrench is factory
adjusted to 30Ncm. Do not
change this value.
*D
6'<<%="3@BJ$"%B<1#$"%#FS@%T3""FS%1@%J1HL
,L*DU%%'@=1<%=I$%23""1$"%1B%J"$$%3B%BIFS@%1@
J1HL%,L*+
Fig. 2.15: Pulling transfer slider down.
Fig. 2.16: Transfer slider is down.
*+
]$(FY$%="3@BJ$"%B<1#$"%2F(?<$=$<A%J"F(%=I$
?F#%)A%?'<<1@H%1=%3S3A%3B%1@#123=$#%%)A%=I$
3""FS%1@%J1HL%,L*\L
Fig. 2.17: Removing transfer slider.
*\
5F'@=%<F3#$#%J<3=%B?$21($@%?F#%F@%=I$%<F3#1@H
#$Y12$%3B%BIFS@%1@%J1HBL%,L*/%:%,L,-[
Fig. 2.20: Bayonet pod is mounted on the bayonet
loading device.
2.4 Mounting pod on bayonet
loading device.
Fig. 2.18: Bayonet connections of pod and loading
device.
Fig. 2.19: Mounting the bayonet pod on the
bayonet loading device.
,L%9'"@%)3AF@$=%?F#%2F'@=$"2<F2OS1B$%=F
<F2O%)3AF@$=
6F#%3@#%<F3#1@H%#$Y12$%I3Y$%(3=2I1@H
)3AF@$=%2F@@$2=1F@BL
9I$%)3AF@$=%?F#%1B%@FS%<F2O$#%=F%=I$
)3AF@$=%<F3#1@H%#$Y12$%3@#%"$3#A%JF"%'B$L
*L%6'BI%)3AF@$=%?F#%1@=F%]9>%<F3#1@H
#$Y12$L
The specimen is
now ready for
freezing in the
EM PACT2.
*/
R1=I%=I$%%=F"K'$%S"$@2I%='"@
2F'@=$"2<F2OS1B$%=F%F?$@%=I$%#13(F@#
B2"$S%1@%=I$%?F#%3B%BIFS@%1@%J1HL%.L,L
3 Unloading
Fig. 3.1: Pod in unloading station.
Fig. 3.2: Turning torque wrench counterclockwise
to open.
Fig. 3.3: Specimen carrier in container.
M@%=I$%0&,%)3=I%FJ%45%6789,N%="3@BJ$"
?F#%1@=F%'@<F3#1@H%B=3=1F@%3@#%%#1B2F@@$2=
)3AF@$=%<F3#1@H%#$Y12$%TJ1HL%.L*:%.L.U[
8F<<$2=%B?$21($@%23""1$"B%1@%2F@=31@$"
TJ1HL.L.UL
9'"@%)3AF@$=%<F3#1@H%#$Y12$%2<F2OS1B$%=F
'@<F2O%)3AF@$=%3@#%"$(FY$L
The specimen is
now ready for
the follow-on
procedures
(e. g. FS).
All tools must be precooled
before touching the frozen
carrier.
Dry pod e.g. on a hot plate
(max. 95 °C)% before next
use.
*V
4 Maintenance
4(?=A%B?$21($@%?F#B%23@%)$%2<$3@$#
3J=$"%'B$%1J%@$2$BB3"A[
]$(FY$%#13(F@#%<F2O1@H%B2"$SL
>F@123=$%?F#B%S1=IF'=%B2"$S%%1@%\-_
4=I3@F<%JF"%3%J$S%B$2F@#BN%=I$@%S3BI%1@
#1B=1<<$#%S3=$"L%a"A%F@%IF=%?<3=$
T(3PL%VD%W8U%)$JF"$%@$P=%'B$L
9I$%J"F@=%J32$%FJ%=I$%#13(F@#%23@%)$
2<$3@$#%S1=I%\-_%4=I3@F<%F@%J1<=$"%?3?$"N
=I$@%S3BI$#%1@%#1B=1<<$#%S3=$"%3@#%31"
#"1$#L
9I$%="3@BJ$"%B=3=1F@%3@#%3<<%F=I$"
2F(?F@$@=B%23@%)$%2<$3@$#%S1=I%\-_
4=I3@F<N%=I$@%S3BI$#%1@%#1B=1<<$#%S3=$"L%%71"
#"A%F"%#"A%F@%IF=%?<3=$%T(3PL%VD%W8U%)$JF"$
@$P=%'B$L
Diamond locking screws
must not be sonicated.