leica dmi8 a leica dmi8 c leica dmi8 m

Installation Manual

Leica Microsystems CMS GmbH • Manual 11934095, Revision 1.0 from 2015-01-15

Leica DMi8 ALeica DMi8 CLeica DMi8 M

Coyprights

Revision 1.0, published on 2015-01-15 by:

Leica Microsystems CMS GmbHErnst-Leitz-Strasse 17-37D-35578 Wetzlar, Germanyhttp://www.leica-microsystems.comResponsible for contents:Marketing CMS

Copyright

All rights to this documentation are held by Leica Micro-systems CMS GmbH. Reproduction of text or illustrations (in whole or in part) by print, photocopy, microfilm or other method (including electronic systems) is not allowed with-out express written permission from Leica Microsystems CMS GmbH.

The instructions contained in the following documentation reflect state-of-the-art technology. We have compiled the texts and illustrations as accurately as possible. Still, we are always grateful for comments and suggestions regard-ing potential mistakes within this documentation.

The information included in this manual may be changed with-out prior notice.

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Contents

Contents

1. Important Notes about this Manual .......................... 41.1 Text symbols and their meanings ............................... 4

2. Safety Notes .................................................................. 62.1 General safety notes .................................................... 62.2 Technical data ............................................................... 6

3. Overview of Components ............................................ 8

4. Unpacking .................................................................... 12

5. Assembling the Microscope .................................... 145.1 Assembling the DIC Module

and DIC objective prisms ........................................... 155.2 Assembling the specimen stage .............................. 165.3 Inserting the eyepieces ............................................. 185.4 Inserting the objectives ............................................. 185.5 Assembling the incident light illuminator ................ 195.5.1 Assembling the LED lamp housing ........................... 195.5.2 Assembling the fluorescence illuminator ............... 195.6 Equipping the incident light reflector turret ........... 205.7 Assembling the polarizer and analyzer ................... 215.8 Assembling the transmitted light illuminator arm (TL) ..................................................... 225.8.1 Tiltable transmitted light illuminator arm ................ 225.8.2 Fixed transmitted light illuminator arm .................... 235.9 Assembling the condensers

for the tiltable transmitted light illuminator arm .... 245.10 Assembling the transmitted light lamp housing ..... 305.11 Wiring ............................................................................ 315.11.1 Wiring for microscopes with

transmitted light illuminator arm .............................. 32

6. Startup .......................................................................... 336.1 Adjusting the diaphragms

for the incident light illuminator ................................ 336.2 Inspecting the phase contrast ring ......................... 346.3 Setting the polarizer ................................................... 366.4 Condenser centering .................................................. 37

7. Index ............................................................................. 38

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1. Important notes about thIs manual

1. Important Notes about this Manual

Numbers in parentheses, e.g. (Fig. 1.2), refer to illustrations, in the example to Fig. 1, Item 2.

Numbers with an arrow, e.g. → p. 20, refer to a specific page of the manual.

WARNING indicates a hazard with a medium degree of risk that, if not avoided, can result in death or serious injury.

CAUTION indicates a hazard with a low degree of risk that, if not avoided, can result in minor or moderate injury.

Caution!In this manual, additional safety notes are indicated with the triangle symbol shown here, and have a gray back-ground.

Caution! The instrument and accessories can be damaged when operated incorrectly.

Caution!

This Installation Manual is an essential component of the product; it must be read carefully before the product is assembled, put into operation or used, and must be kept for later reference.

This Installation Manual includes important instructions and information related to the operating safety and mainte-nance of the instrument.

1.1 Text symbols and their meanings

(Fig. 1.2)

→ p.20

!

WARNING

CAUTION

4

1. IMPORTANT NOTES ABOUT THIS MANUAL

Warning of hazardous electrical voltage! Danger of electric shock!

Danger due to hot surface

Warning of optical radiation! Never look directly into the light beam! Wear safety goggles!

Warning of electromagnetic fi eld

Warning of UV radiation! Wear safety goggles!

Warning of hazardous laser radiation – take appropriate safety precautions!

Warning of hand injuries

Instructions for disposing of the instrument, its acces-sories and consumables.

Connection to ground

Explanatory note

Item not contained in all equipment confi gurations

Manufacturing date, e.g. 11 / 2011 for November 2011

China RoHS 50 years EFUP(Environmentally friendly use period)

*

MM/YYYY

5

2. Safety NoteS

2. Safety Notes

2.1 General safety notes

Caution!

Make sure to follow the safety notes in the Leica DMi8 User Manual.

Caution!

Also see the instructions and warnings contained in this Installation Manual.

Caution!

The instruments and accessories described in this In-stallation Manual have been safety-tested and checked for possible hazards.The responsible Leica representative or the main plant in Wetzlar must be consulted whenever the device is altered, modified or used in conjunction with non-Leica components that are outside of the scope of this manual!

Unauthorized alterations to the instrument or noncom-pliant use shall void all rights to any warranty claims!

2.2 Technical data

Compact Leica CTR electronics box

For indoor use only.Supply voltage: 100 – 240 VACFrequency: 50 / 60 HzPower consumption: max. 150 VAFuses: 3.15 A, slow-blowing, breaking capacity H, 250 VAC Size: 5x20 mmAmbient temperature: 15° - 35°CRelative humidity: 90% up to 30°C, non-condensingProtection class: IOvervoltage category: IIPollution degree: 2

Advanced/Advanced+ Leica CTR electronics box


6

2. Safety NoteS

Leica DMi8 M microscope (manual)


Leica DMi8 C microscope (manually encoded)


Leica DMi8 A microscope (automated variants)

For indoor use only.Supply voltage: 100 – 240 VAC (→ Leica CTR)Frequency: 50 / 60 Hz (→ Leica CTR)Power consumption: see Leica CTRFuses: see Leica CTRAmbient temperature: 15° - 35°CRelative humidity: 90% up to 30°C, non-condensingProtection class: I (→ Leica CTR)Overvoltage category: II (→ Leica CTR)Pollution degree: 2 (→ Leica CTR)

Leica EL6000*

For indoor use only.Supply voltage: 100 – 240 VACFrequency: 50 / 60 HzPower consumption: max. 200 VAFuses: 2.5 A, slow-blowing, breaking capacity H, 250 VAC Size: 5x20 mmAmbient temperature: 0° - 40°CRelative humidity: 90% up to 30°C, non-condensingProtection class: IOvervoltage category: IIPollution degree: 2(* See provided Instructions for Use)

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3. Overview Of COmpOnents

Fig. 1: Right side of the Leica DMi8 A

7 Aperture diaphragm8 Field diaphragm9 Polarizer slot10 Analyzer slot11 Cover for right side-port12 Cover for motorized incident light turret disk

3. Overview of Components

1 Objective turret with objectives2 Tube3 Eyepieces and eyepiece tubes4 Specimen stage5 LED lamp housing6 Colour filter slot

5

6

1

8 7

10

42

3

912 11

8


Fig. 2: Left side of the Leica DMi8 A

1 Motorized DIC objective prism disk2 Cover plate (not used)3 Service port4 Left side-port with camera

1

34

2

9


Fig. 3: Left side of the Leica DMi8 C

1 Eyepieces and eyepiece tubes2 Tube3 Top-port with camera4 Cover plate (not used)5 Incident light turret disk6 Cover for left side-port7 Manual DIC objective prism disk8 LED lamp housing 9 Objective turret with objectives10 Specimen stage

1

2

4

67

8

9

10

5

3

10


Variants with transmitted light illuminator armFig. 4: Left side of the Leica DMi8 with tiltable transmitted light illumi-

nator arm1 Tiltable transmitted light illuminator arm2 Condenser height adjustment3 Manual polarizer4 Manual condenser, alternatively: motorized condenser5 Condenser head6 Condenser height stop

1

3

4

2

56

Fig. 5: Right side of the Leica DMi8 with tiltable transmitted light illuminator arm

1 Transmitted light filters2 Transmitted light lamp housing receptacle3 Condenser with motorized polarizer

Fig. 6: Left side of the Leica DMi8 with fixed transmitted light illumina-tor arm

1 Stop lever for condenser height adjustment2 Fixed transmitted light illuminator arm3 Transmitted light lamp housing4 Receptacle for transmitted light filter5 Light ring slide6 Manual condenser with aperture diaphragm

2

1

3

4

6

51

3

2

11

4. Unpacking

4. Unpacking

The microscope is delivered in several packages depend-ing on instrument configuration and the scope of the sys-tem.

The Leica CTR electronics box, Leica SmartMove control el-ement, Leica STP4000/8000 control panel, movable stages/stage accessories and Leica EL6000 compact light source are delivered in separate packages.

Caution!

The microscope may only be set up, installed and put into operation by authorized Leica personnel!

Caution!

The microscope may only be operated by personnel who have been instructed by an authorized Leica employee!

Please compare the contents of the package you receive carefully with the packing slip, the delivery note or the in-voice. We highly recommend keeping a copy of these docu-ments with the manual in order to have delivery date and delivery package information available when (for example) placing repeat orders or making service calls. Please make sure that no small parts are lost in the packaging materials. Some of our packaging materials have symbols to help in environmentally responsible recycling.

First, carefully remove all components from the transporta-tion and packaging materials.

Caution!

If an instrument or its packaging have visible damage, do not put the instrument into operation.

Note:

If at all possible, avoid touching optical surfaces such as the lens surfaces on the objective. If fingerprints do appear on the glass surfaces, remove them with a soft leather or linen cloth. Even small traces of finger perspiration can damage the surfaces in a short time. See the chapter on "Care of the Microscope"→ for additional instructions in the Instructions for Use.

Caution!

Do not connect the microscope and peripherals to the power supply at this point under any circumstances!

Installation location

Work with the microscope should be performed in a dust-free room, which is free of oil vapors, other chemical va-pors and extreme humidity. At the workstation, large tem-perature fluctuations, direct sunlight and vibrations should be avoided. These conditions can distort measurements and longer microscopic scans.

Permitted ambient conditions:Temperature 15–35°CRelative humidity maximum 90% up to 30°C non-condensing Microscopes in warm and warm-damp climatic zones re-quire special care in order to prevent fungus contamination. For additional instructions, see the chapter on "Care of the Microscope" in the→ User Manual.

Caution!

The microscope must be set up so that the power volt-age input is not obstructed and the instrument can quickly be removed from the power supply if necessary.

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4. Unpacking

Caution!

Electrical components must be placed at least 10 cm away from the wall and away from flammable substances.

Weight

The weight of the microscope depends on the respective equipment configuration.

CAUTIONAt a full load, the microscope weighs more than 18 kg. The user must take corresponding precautions for trans-porting the instrument.

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5. Assembling the microscope

The microscope components +) are logically assembled in this order:

• Only for microscopes with transmitted light illuminatorarm (chapter 5.8-5.10):• Transmittedlightilluminatorarm• Condenserwithcondenserhead• Transmittedlightlamphousing

• DICmoduleandDICobjectiveprisms• Specimenstage• Eyepieces• Objectives• Incidentlightilluminator(LEDlamphousing)• Equipmentfortheincidentlightturret• Polarizerandanalyzer

When using other systems and advanced optical accesso-ries, the sequence may vary.

Only a few commonly used screwdrivers and keys are necessary for assembly; these are included in the delivery package.

5. Assembling the Microscope

+) Depending on scope of delivery

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5. Assembling the microscope – Dic moDule AnD Dic objective prisms

5.1 Assembling the DIC Module and DIC objective prisms

If the microscope is not equipped with DIC, please jump ahead to Chapter 5.2.In microscopes of the Leica DMi8 M series, the DIC prisms are already installed in the DIC disk (Fig. 7) below the objec-tive turret. Motorized, manually encoded and manual DIC disks are offered. Assembly is identical for all variants.

When upgrading the IC prism disk, follow these steps:

• Removethefrontpanel(Fig.8)undertheobjectiveturretafter loosening the Allen screws (Fig. 9).

• Insert theDIC prism disk (Fig. 7) evenly into the recep-tacle. Start by gently turning one screw using the 3 mm hexagonal key provided. Then, tighten both Allen screws.

! Caution!

Insert the prism disk so that the electronics board is fac-ing downwards. Do not touch the electronics (especially the contacts) with your fingers!

Upgrading individual IC prisms:

• LoosenbothAllenscrewsandremovetheprismdisk.

• Place theprismsagainst thepeg (Fig.7.3)and theshimbetween the prism and screw (Fig. 10) and lightly tighten to avoid tension. Insert the prisms so that the identifying letter, or ID, is facing upwards and is legible.

• Afterassemblingtheprisms,reinsert theprismdisk intothe receptacle.

Alternatively:

Insert the objective prism slide (Fig. 11) into the slot below the objective turret. The code letter must match the code letter on the objective.

Fig. 7: DIC objective prism turret (encoded and motorized)1 IC objective prism in mount2 Identifying letter (ID)3 Orientation pin

3 2 1

Fig. 8: Front panel for DIC prism disk

Fig. 9: Removing the front panel

Fig. 10: IC objective prism1 Objective prism in mount2 Washer and screw

2 1Fig. 11: Objective prism slide

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5. Assembling the microscope – specimen stAges

5.2 Assembling the specimen stage

There is a wide variety of available specimen stages. The accompanying figures depict a few examples.

The assembly of each of these stages is identical. The stages are permanently affixed to the microscope using 3 screws (Fig. 14). In the case of fixed stages, a mechanical stage can be attached (Fig. 15).

! Caution!

Screw lengths may vary. The following applies to the ship-ment of different screws: Always use the shorter of the 3 screws for the front hole and both screws of equal length for the rear holes.

• Alignthestagesothatbothholespointbacktowardstheillumination axis and the single hole is facing in the direc-tion of the tube.

• Align the fastening holes in the stagewith the holes inthe contact surface. If the holes are not visible, as in the case of the 3-plated cross-stage or scanning stage, gen-tly move the upper tabletop until the opening is visible.

• Startbygentlyturningthefrontscrewusingtheprovided3 mm hexagonal key. It is important that the shorter of the 3 screws is always placed in the front hole, because a screw that is too long can damage the focus hub.

• Then,tightenbothrearscrews.

• Finally,retightenthefrontscrew.

Fig. 12: Fixed stage

Fig. 13: Mechanical 3-plate stage

Fig. 14: a, b assembly screws for 3-plate cross-stage

a b

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5. Assembling the microscope – specimen stAges

Fixed stage

Mechanical stages for fixing holders for various culture flasks are also optionally available for fixed stage plates (Fig. 15).

• The mechanical stage has 2 screws. Tighten thesescrews into the threaded holes on the bottom of the fixed stages using the 3 mm hexagon socket wrench and re-tighten regularly after frequent use of the mechanical stage.

The mechanical stage is preadjusted at the factory. If the mechanical stage shifts out of focus when moving from right to left, this can be corrected by Leica Technical Ser-vice.

• Now,removetheorderedstageinsertorinserts(Fig.16)from the packaging and guide one stage insert into the precise click system. The stage, mechanical stage and stage insert are now ready to use.

Self-adhesive scales for reading the coordinate adjustment are included with some (not all) inserts.

• Finally,affixthesescalesintherecessesofthemechani-cal stage.

Motorized 3-plate or scanning stage

3-plate stages and scanning stages: • Afterassemblingthestage,firstconnect (in thecaseof

motorized stages) the provided stage cable to the socket on the stage and then to the electronics box. The corre-sponding mark on the box is called "XY- Stage".

Various inserts are offered with the normal 3-plate or scan-ning stages (including heated inserts).

• Carefully place these inserts into the corner providedwith spring clips from above at an angle. The stage in-serts click into place once positioned correctly.

! Caution!

Only press on the spring clips from the sides. Never press on the insert plate at the spring clips from

above at an angle, because then the insert will not be plane-parallel to the stage and cannot be bent.

Fig. 15: Mechanical stage for fixed micromanipulation stage

Fig. 16: Inserts for mechanical stage (fixed stage) For additional examples, see special brochure

17

5. Assembling the microscope – eyepiece And objectives

5.3 Inserting the eyepieces

• Inserttheeyepiecesintotheeyepiecetubes.

Note:

We recommend using the LAS software to teach in eye-pieces that are not included in the scope of delivery.This ensures that the total magnification specified on the touchscreen is correct.See LAS software manual:

Fig. 17: Eyepieces

5.4 Inserting the objectives

• Screwtheobjectivesintotheobjectiveturret. The receptacles on the objective turret are numbered

(Fig. 18). Depending on your equipment, specific positions have already been assigned to the individual objectives at the factory.

For details on the exact positions of the objectives, please refer to the enclosed "Identification sheet".

! Caution!

Close vacant threads in the turret using dust protection caps!

Please note that the front lenses of the objectives face up-wards. This subjects them to increased exposure to con-tamination compared to objectives on upright microscopes.Check the front lens more often for cleanliness for this rea-son.

Note:

We recommend carrying out a parfocality adjustment via the LAS software.See LAS software manual:

Fig. 18: Objective turret

Fig. 19: Objective turret, equipped

18

5. Assembling the microscope – illuminAtion

5.5 Assembling the incident light illuminator

Caution!

Ensure that the lamp housing has been disconnected from the power supply. Unplug the power plug and the power supply during assembly.

CAUTIONLight sources pose a potential irradiation risk (blinding, UV radiation, IR radiation). Therefore, lamps may only be operated in closed housing and in an installed condition.Never look directly into the beam path (blinding hazard).

5.5.1 Assembling the LED lamp housing

• Placethelamphousingintotheincidentlightlamphous-ing receptacle (Fig. 20) and fasten it with the side clamp-ing screw.

Leica DMi8 M with manual incident light method:• Connect the cable of the lamp housing to the port (Fig.

21.1) on the rear side of the microscope.

Leica DMi8 A and C with motorized incident light method:• Connectthecableofthelamphousingtothepowersup-

ply for transmitted light for the Leica CTR electronics box ("Lamp RL" port).

1

Fig. 20: Fastening the lamp housing to the incident light axis1 Fastening screw

5.5.2 Assembling the fluorescence illuminator

Leica EL6000

See separate manual.

CAUTIONWhen using the Leica EL6000 compact light source, it is essential to observe the safety notes in the separate in-structions included.

CAUTION

Just switch on the light source once the light guide is connected to the microscope.Uncontrolled light output from the light guide poses a blinding hazard!There is also a burn hazard at the light guide output!

• ScrewtheLeicaEL6000microscopeadapterontotherearside of the microscope in the lamp housing receptacle.

• Closethe lightguideaccordingto the information in theLeica EL6000 manual.

Fig. 21: Manually connecting the lamp housing for the Leica DMi81 Incident light lamp housing connection

1

19

5. Assembling the microscope – incident light turret equipment

5.6 Equipping the incident light reflector turret

! Caution!

First, completely read through this section before you begin equipping the reflector turret disk.

The reflector turret disk can be accessed from both the right side and the left side of the microscope (Fig. 22).

The positions on the reflector turret disk are numbered. De-pending on how they are equipped, the individual filter and/or reflector cubes are set in pre-assigned positions at the factory. For details, check the identification sheet included with your order.

•Removethecoverfromthereflectorturretdisk(Fig.22.1).The cover is secured in place by a magnetic holder and can be detached from the front.

• Withtheholderfacingyousquarely,slidethefiltercubeor reflector cube forwards into the holder as described in the identification sheet provided.

•Checktoensurethatthecubeisplacedproperly.Aloosecube can be damaged when rotating the turret disk or can block the disk.

• Onceallfilterblocksandreflectorblockshavebeen in-serted, put the cover back in place.

Note:

Is the microscope equipped with a 2-fold filter turret, the turret must be pushed across the arrester position to ex-change the filter cubes. It is not possible to insert the filter cubes when the turret is in the arresting position.

1

Fig. 22: Access to turret disk1 Cover

Fig. 23: Equipping the turret disk

Fig. 24: Turret disk with used filter cubes

20

5. Assembling the microscope – polArizer And AnAlyzer

5.7 Assembling the polarizer and analyzer

Manual incident light polarizer

• Remove theplugcapon therightsideof thestand (be-hind the incident light turret disk (Fig. 25.1)).

• Insert thepolarizer into thereceptacleuntil it latches inplace (Fig. 25.1).

Manual incident light analyzer

• Removetheplugcapontherightsideofthestand(belowthe incident light turret disk (Fig. 25.2)).

• Insert theanalyzer into the receptacleuntil it latches inplace (Fig. 25.2).

Fig. 25: 1 Incident light polarizer2 Incident light analyzer

1

2

21

5. Assembling the microscope – trAnsmitted light illuminAtion cArrier

5.8 Assembling the transmitted light illuminator arm (TL)

5.8.1 Tiltable transmitted light illuminator arm

• Wipeoffthemicroscope’scontactsurface(Fig.26.3)witha dry cloth.

• Lightly tilt the illuminator arm (Fig. 26.1) to the rear andinsert it so that the pin (Fig. 26.2) fits in the groove of the contact surface (Fig. 26.4).

• Raiseupthetransmittedlightilluminatorarmandfastenitwith 4 screws (Fig. 27.1).

When fastening, do not hold onto the transmitted light il-luminator arm. This ensures optimum alignment for the optical axis.

• Thetiltingangleoftheilluminatorarmcanbemodifiedorlocked in place in the vertical position using the knurled screw (Fig. 27.2).

Leica DMi8 A• Connect theelectronicscable to oneof theEXT1–EXT6

sockets (Fig. 27.3).

Fig. 26: Transmitted light illuminator arm assembly

1 Transmitted light illuminator arm2 Pin on transmitted light illuminator

arm3 Contact surface4 Groove on contact surface5 Connecting cable

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4

5

2

Fig. 27: Rear side of motorized Leica DMi8 1 Fastening screws on the transmitted light illuminator arm2 Knurled ring for locking the transmitted light llluminator arm in

place3 EXT1-EXT6 sockets

1 2

3

1

22

5. Assembling the microscope – trAnsmitted light illuminAtion cArrier

5.8.2 Fixed transmitted light illuminator arm

Assembling the transmitted light column

• Attachthetransmitted light illumination column (Fig. 30.1) and fasten it with 4 screws (Fig. 30.2).

Attaching the condensers

• Screw the S80/0.30 condenser (Fig. 28.2) or S40/0.45 condenser (Fig. 28.1) onto the condenser holder of the (Fig. 29.2) transmitted light illuminator arm from below.

Fig. 28: Condensers1 S40/0.45 condenser2 S80/0.30 condenser

1 2

Inserting the transmitted light illuminator arm

• Insert the transmitted light illuminator arm into the col-umn from above by pressing and holding down the stop lever for condenser height adjustment (Fig. 29.5).

• Position the transmitted light illuminator arm (Fig. 29.3)onto the transmitted light illumination column based on the condenser being used (S40/0.45 or S80/0.30) (Fig. 29.4) and release the stop lever. (Also see the following note.)

The marks (Fig. 29.6) pertain to a liquid height of 15 mm. For stands with double marks, the upper and lower lines represent a range with different liquid heights.

• Make sure that the transmitted light illuminator arm is engaged.

• Connecttheelectronicscabletotheport(Fig.30.3).

Note:

For the stands with 3-plate mechanical stages, position the illuminator arm 25 mm below the marks, as the illumination axis is installed 25 mm higher on an adapter than on the fixed stage.

Note:

A screw on the transmitted light illumination column pre-vents the condenser from colliding with the specimen stage. If necessary, move the screw into the higher of the two openings, if using the S80/0.30 condenser.

2

1

6

6

3

5

4

Fig. 29: Transmitted light illumination unit with condenser1 Condenser2 Condenser holder3 Transmitted light illuminator arm4 Transmitted light illumination column5 Stop lever for condenser height adjustment6 Labels

Fig. 30: Assembling the transmitted light illumination column1 Transmitted light illumination column2 Fastening screws3 Cable connection

1

2

3

23

5. Assembling the microscope – condenser

5.9 Assembling the condensers for the tiltable transmit-ted light illuminator arm

For the fixed transmitted light illuminator arm, see Chapter 5.8.2 → Page 23.

All condensers from the Leica DMi8 series with a tiltable transmitted light illuminator arm are equipped with a 7x turret disk and can be individually equipped with the cor-responding ring diaphragms for phase contrast (PH), dark field (DF) or IC prisms for transmitted light differential inter-ference contrast (DIC).

Usually the light rings and condenser prisms have already been inserted into the turret disk at the factory, eliminating the need for subsequent assembly. For more information, read → Page 28: Assembling condensers.

Fig. 31: S1-S28 condenser base

Fig. 32: S1 condenser head

Fig. 33: S28 condenser head

Fig. 34: Phase rings

Fig. 35: Condenser prisms

24


Assembling light rings

• Switchthemicroscopeoff.

• Removethecondenserdoor(Fig.36.1).Thelightringisin-serted into one of the large receptacles of the condenser disk featuring guide grooves.

• Using the adjusting key, turn the right centering screwof the condenser disk (Fig. 37.2) all the way back. In or-der to prevent the condenser disk from rotating, insert the adjusting key (Fig. 37.2) into the left centering screw of the disk. This may protrude a maximum of 1 mm into the opening.

Insert the light rings for phase contrast (marked with the identification numbers 0, 1, 2 and 3) and the S focal intercept of the corresponding condenser head) and DF diaphragm (marked with D for dark field and the S focal intercept of the corresponding condenser head) into the receptacle drill holes of the turret disk as follows:

• Selectanopeningandchecktoensurethatbothfasten-ing screws are loose enough so that they are no longer protruding into the opening. Move the desired light ring opening into the beam path opening to adjust the screws. Now, you can turn the screws using both adjusting keys.

• Now,pickupthespecialcondenserclamp(Fig.37.1).

• If possible, install light rings 0 to 3 in ascending order. You will find numbering for the openings on the edge of the gear rim (4 large openings: 1-4; 3 small openings: 5-7).

Fig. 36: Condenser1 Condenser door2 Centering hole

1

2

Fig. 37: Opened condenser1 Condenser clamp2 Adjusting key

1

1

2

25


• Using the condenser clamp, grip the light ring to be in-stalled (the marking must be facing upwards and be leg-ible), so that the cross piece of the light ring stands at thecenterof theclamp’s lugand theupperedgeof thelight ring is lying flat in the clamp holder. The numbering should point to the top of the clamp. The light ring can be grFiged by pressing down on the side prongs of the clamp (Fig. 38).

•Twoguidingridgescanbefoundonthebottomofthelight rings. These ridges must fit into the two grooves in the opening.

The light ring must be inserted (condenser clamp from above at a slight angle and at an angle of approx. 90° to the housing) so that the mount is grips under the spring clip of the holder (Fig. 38).

! Caution!

Never press down on the spring clip. This can destroy the clip or put the light ring in an unstable position.

Make sure that the light ring clicks into place (through ro-tation) and loosen the clamp.

If necessary, carefully remove fingerprints (or dust) from the prism.

• The light ring is precentered using the left centeringscrew. The right centering screw must never limit the range of movement.

• Writedownthenumberoftheopeningandthelightringdesignation so that the LAS software can be adjusted lat-er.

• Removetheadjustingkeyandclosethecondenseragain.

• Centeringprecisionisachievedusingthetelescopicunitafter the microscope has been switched off.

For information on installing IC condenser prisms, continue reading. Otherwise, jump ahead to Page 28 Assembling condensers.

Fig. 38: Inserting the prismThe designation must be visible when inserted and oriented to the cen-ter of the condenser disk.Otherwise, a DIC image cannot be produced.

26


Assembling IC condenser prisms

• Switchthemicroscopeoff.

• Remove the condenser door (Fig. 36.1). The prism is in-serted into one of the large receptacles of the condenser disk featuring guide grooves.

• Using the adjusting key, turn the right centering screwof the condenser disk (Fig. 37.2) all the way back. In order to prevent the condenser disk from rotating, in-sert the adjusting key (Fig. 37.2) into the left center-ing screw of the disk. This may protrude a maximum of 1 mm into the opening.

• Usingthecondenserclamp,griptheprismtobeinstalled(the marking must be facing upwards and be legible), so that the cross piece of the prism ring stands at the cen-ter of the clamp’s lug and the upper edge of the prismis lying flat in the clamp holder. The K2-K16 numbering should face towards the tip of the clamp. The prism can be grFiged by pressing down on the side prongs of the clamp (Fig. 38).

• Two guiding ridges can be found on the bottom of theprisms. These ridges must fit into the two grooves in the opening.

The prism must be inserted (condenser clamp from above at a slight angle and at an angle of approx. 90° to the housing) so that the mount is grips under the spring clip of the holder (Fig. 38).

! Caution!

Never press down on the spring clip. This can destroy the clip or put the prism in an unstable position.

Make sure that the prism clicks into place (through rota-tion) and loosen the clamp.

If necessary, carefully remove fingerprints (or dust) from the prism.

• Theprismisprecenteredusingtheleftcenteringscrew.The right centering screw must never limit the range of movement.

• Writedownthenumberoftheopeningandtheprismdes-ignation so that the LAS software can be adjusted later.

• Removetheadjustingkeyandclosethecondenseragain.

• Centeringprecisionisachievedusingthetelescopicunitafter the microscope has been switched off.

27


Assembling condensers

Assembly is the same for all S1 to S70 condensers (motor-ized or manual/encoded; not encoded for S40).

• LoosentheAllenscrewsontherightsideofthecondens-er holder.

• Place the condenser on top of the locking pins of thetransmitted light illuminator arm and then move the con-denser to the corresponding height. Marks on the column and the condenser help you in finding the correct dis-tance.

• TightentheAllenscrewonceyouhavereachedthecor-rect position.

Fig. 39: Assembling the condenser on the transmitted light illuminator arm

Fig. 40: Condenser mounted on transmitted light illuminator arm

28


Condenser heads

There are 4 different condenser heads available:

1) S1/1.40 oil2) S1/0.90 dry3) S23/0.534) S28/0.55

Condenser head 1 and 2:• Screwthespacerring(Fig.41.2)intothethreadedholeon

the bottom of the condenser base.

• ScrewtheS1condenserheadintothisspacerring.

Condenser head 3 and 4:• Screwthecondenserdirectlyintothecondenserbase.

The S40 and S70 condensers are delivered complete with condenser heads, so no additional assembly is necessary.

Fig. 41: Inserting the S1 condenser heads1 Condenser base2 Spacer ring3 Condenser head

Fig. 42: Inserting the S28 condenser head

1

2

3

Assembling the polarizer

Preconfigured at the factory.

Proceed as follows when performing upgrades:

Motorized condenser:

See the enclosed Assembly manual.

Manual condenser:

• Fasten the 1-position or 3-position position holder ontothe top of the manual condenser as necessary. The holder has a guide that must be inserted into the opening next to the screw thread. The holder must be positioned so that the polarizer to be inserted or the filter covers the opening of the condenser (Fig. 44).

• Now,insertthepolarizerorfilterintotheholderwiththedesired side facing upwards (λ: lambda and polarizer; POL: only polarizer). A clicking mechanism indicates tells you when it is positioned correctly. It must be possible to turn the polarizer slightly in the guide (approx. 30°) until it reaches both stops.

Fig. 43: Condenser with motorized polarizer

Fig. 44: Condenser with manual polarizer

29

5. Assembling the microscope – illuminAtion

5.10 Assembling the transmitted light lamp housing

Caution!

Ensure that the lamp housing has been disconnected from the power supply. Unplug the power plug and the power supply during assembly.

CAUTIONLight sources pose a potential irradiation risk (blinding, UV radiation, IR radiation). Therefore, lamps may only be operated in closed housing and in an installed condition.Never look directly into the beam path (blinding hazard).

For the tiltable transmitted light illuminator arm:

• Place the lamp housing into the transmitted light lamphousing receptacle (Fig. 45) and fasten it with the side clamping screw.

• Routethecableintothecableshaftontherearsideofthetransmitted light illuminator arm (Fig. 46).

Leica DMi8 M and C with manual transmitted light method:• Connect the cable of the lamp housing to the port

(Fig. 47.1) on the rear side of the microscope.

Leica DMi8 A with motorized transmitted light method:• Connectthecableofthelamphousingtothepowersup-

ply for transmitted light for the Leica CTR electronics box (“Lamp TL” port).

1

Fig. 45: Fastening the lamp housing to the transmitted light illuminator arm

1 Fastening screw

Fig. 46: Wiring in the lamp housing (cable shaft)

Fig. 47: Manually connecting the lamp housing for the Leica DMi81 Transmitted light lamp housing connection

1

30

5. Assembling the microscope – Wiring

5.11 Wiring

WARNING

Only use power cables or alternative cables with VDE/HAR codes that minimally fulfill the following conditions: 3x0.75 mm2 and 10A/250V.

The microscope can be operated with or without an elec-tronics box depending on its configuration. Depending on the microscope configuration, different electronics boxes are available:

• LeicaCTRcompact electronics box• LeicaCTRadvancedelectronicsbox

Note:

The electronics box may not be used with any other stands. The serial number of the corresponding stand is noted on the rear side of the electronics box.

Leica DMi8 M/C

• Connectthelampcabletotheport(Fig.48.1).

• Connect themicroscope to the power supply using thesupplied power cable (Fig. 48.2).

1

2

Fig. 48: Rear side of Leica DMi8 M1 Lamp cable connection2 Socket for power cable

Leica DMi8 A

• Connecttheincidentlightilluminatorport(Fig.49.3)oftherear side of the microscope to the Lamp RL port (Fig. 50/ Fig. 51.1) on the electronics box using the lamp cable.

• Connect theMicroscope socket (Fig. 50/Fig. 51.4) to the rear side of the stand (Fig. 49.1). Use the 25-pin micro-scope cable for this.

• Ifpresent,connecttheLeicaSmartMovecontrolelementor Leica STP4000/8000 control panel to one of the USB ports (Fig. 50/Fig. 51.3).

• If necessary, connect the motorized stage to the XY-Stage socket (Fig. 51.6).

• Theconnectionforadditionalmotorizedcomponentsde-pends on the configuration.

• Aftercompletingallof theassemblywork, theelectron-ics box is connected to the power supply using the power cable provided (Fig. 50/Fig. 51.5).

Caution!

To prevent the sockets from overheating, ensure that the plugs are inserted and screwed in correctly.

3

21

Fig. 49: Rear side of Leica DMi8 A 1 CTR electronics box connection2 EXT1-EXT6 connections3 Incident light illuminator connection

31

5. Assembling the microscope – Wiring

5.11.1 Wiring for microscopes with transmitted light illu-minator arm

Leica DMi8 M/C

For the tiltable transmitted light illuminator arm:• Connectthelampcabletotheport(Fig.48.1).

For the fixed transmitted light illuminator arm:• Connect the transmitted light illuminatorarm to theport

(Fig. 48.1).

• Connect themicroscope to the power supply using thesupplied power cable (Fig. 48.2).

Leica DMi8 A

• Connect theelectronicscableof thetransmitted light il-luminator arm to one of the EXT1–EXT6 sockets (Fig. 49.2).

• Connect the lamp cable of the transmitted light illumi-nator arm to the Lamp TL port (Fig. 50/ Fig. 51.2) on the electronics box.

Fig. 50: Rear side of compact Leica CTR electronics box1 Connection for lamp cable for incident light2 Connection for lamp cable for transmitted light3 2 x USB4 Microscope connection5 Socket for power cable

1

3

4

5

2

Fig. 51: Rear side of advanced Leica CTR electronics box1 Connection for lamp cable for incident light2 Connection for lamp cable for transmitted light3 1 x serial, 2 x USB4 Microscope connection5 Socket for power cable6 Motorized stage connection

36

5

4

1 2

32

6. Instrument setup– AdjustIng the dIAphrAgms

6. Startup

6.1 Adjusting the diaphragms for the incident light illuminator

The following procedure is provided for incident light-bright field illumination.

• Select an objective with moderate magnification (10x–20x) and adjust the image.

• Activatetheincidentlightaxisifnecessary.

• SwitchontheBF(=brightfield)orSmithreflector.

• Placeaspecimenonthestage.

•Bringthespecimenintofocusandadjustthelight inten-sity.

Adjusting the field diaphragm

• Closetheilluminatedfielddiaphragmuntiltheedgeofthediaphragm appears in the specimen plane.

•Ifthelimitsofthefielddiaphragmarenotinthecenterofthe field of view, move the position of the field diaphragm to the center with the two centering screws (Fig. 52.1) on the right side of the stand using a 3 mm Allen key. When centering, observe the position of the field diaphragm through the eyepieces.

•Openthelightfielddiaphragmuntilitjustdisappearsfromthe field of view.

•Thissettingofthefielddiaphragmremainsthesameforallobjectives.

It is necessary to readjust the field diaphragm only when using eyepieces with different field numbers or when changing the magnification using a magnification chang-er, and for photography and TV imaging. Narrowing the field diaphragm normally improves the contrast.

21

Fig. 52: Adjusting the diaphragms (incident light)1 Adjusting screws for moving the field diaphragm2 Adjusting screws for moving the aperture diaphragm

Adjusting the Aperture Diaphragm

• Useaflatspecimenwithgoodreflectiveproperties,andaglass plate (specimen carrier) if necessary.

• Removeoneeyepiece (the right one, for example).

• Close theaperturediaphragmuntil theedgeof thedia-phragm appears in the exit pupil of the objective (aper-ture diaphragm plane).

• Iftheimagedoesnotappearinthemiddleofthefieldofview, the aperture diaphragm must be moved into the exit pupil using the centering screws (Fig. 52.2).

• Opentheaperturediaphragmuntilthespecimentappearsin good contrast.

33

6. Instrument setup– InspectIng the phase contrast rIng

6.2 Inspecting the phase contrast ring

For the Leica DMi8 with tiltable transmitted light illumina-tor arm

If your microscope is equipped for the use of phase con-trast, the light rings that fit the objectives are built into the condenser. The light rings are already centered in the fac-tory. As a result of transport and setup of the stand, how-ever, in some cases centering maybe be required again. Therefore check the centering.

Note:

Every objective is assigned its own light ring in the con-denser disk. Therefore, a check must be performed for each objective.

Regular phase contrast with phase objectives

When rotating in an objective that is suitable for phase con-trast, the corresponding light ring is introduced automati-cally (motorized condenser) or you must manually swing the corresponding light ring inwards.

Leica DMi8 with manual condenser:• Ifnecessary,settheTLbright-fieldpositionoremptypo-

sition on the fluo turret, or select the "DAPI-TL" filter cube.

•Selectthebright-fieldpositiononthecondenser.

Leica DMi8 with motorized condenser:• SelectthePH contrast method (phase contrast).

• Instead of an eyepiece, insert the focusing telescope (Fig. 53) into the observation tube.

• Selectthephasecontrastobjectivewiththelowestmag-nification.

• Optimizethefocusonthespecimen.

• Put the ringstructure (Fig. 55a)by into focusbyslightlyloosening the clamping ring (Fig. 53.2) and moving the eye lens (Fig. 53.1).

• Retightentheclampingring.

Fig. 53: Focusing telescope1 Adjustable eyelens2 Clamping ring for fixing the focus position

1

2

34

6. Instrument setup– InspectIng the phase contrast rIng

Leica DMi8 with manual condenser:•Selectthelightringforyouractiveobjectiveonthecon-

denser.

Leica DMi8 with motorized condenser:• Thecorrectlightringonthemotorizedcondenserisauto-

matically swung inwards.

• If the light ring and the phase ring are not shown as ar-ranged in Fig. 55c, the light ring must be centered and congruent.

• Insert the centering key through the openings provided

Fig. 54.3) on both sides of the condenser.

• Turn thecenteringkeyuntil thedark ring (phase ring inthe objective) is congruent with the slightly narrower bright ring (light ring in condenser) (Fig. 55c).

Caution!

When changing the objective, the centering key must no longer be in the corresponding openings. You can lock the condenser.

• Repeat the process for all other phase contrast objec-tives.

• Always remove the centering keys after the centeringprocedure.

Note:

You can find more information on the phase contrast meth-od in Leica Science Lab.Link:http://www.leica-microsystems.com/science-lab/

Fig. 54: Condenser centering1 Centering holes2 Height adjustment3 Prisms and phase ring centering

2 1

3

2 1

3

PH LR

Fig. 55: Centering procedure for phase contrastPH=phasecontrastring,LR=lightringa Condenser in bright field (BF) positionb Condenser in phase contrast (PH) position Light ring (LR) not centeredc Light ring and phase ring centered

a b c

35

6. Instrument setup– settIng the polarIzer

6.3 Setting the polarizer


Start by removing the specimen from the microscope stage.

Leica DMi8 with manual condenser:•Selectthebright-fieldpositiononthecondenser.

• Slidetheanalyzer into the analyzer slot on the right side of the stand.

• Swingthecondenserheadinward.

•Rotatethepolarizer until the optimum extinction position is reached.

Leica DMi8 with motorized condenser and polarizer:• SelectthePOLmethod.If theanalyzer ispresentonthe

fluo turret as an analyzer block, it will automatically swing inwards. A manual analyzer must be manually inserted.

On motorized condensers with motorized polarizers, the polarizers move into position automatically.

• Insertthecenteringkeyintothecorrespondingopeningson the condenser (Fig. 57).

• Settheoptimumextinction(max.darkness).(Theanalyzermust be swung inwards.)

• Removethecenteringkeysagain.

Place the specimen back onto the stage.

1

Fig. 56: Condenser with manual polarizer

Fig. 57: Condenser with motorized polarizer1 Centering holes on the condenser

!

36

6. Instrument setup– Condenser CenterIng

6.4 Condenser centering


Set the Koehler illumination for your microscope (not avail-able for S40 and S70 condensers).The condenser is also pre-adjusted in the factory.However, depending on how the condenser is disassembled and reassembled, it may be necessary to re-center the con-denser in some cases. Therefore, check the condenser cen-tering.

• Adjust the image so it is as sharp as possible by carefully moving the condenser up and down (Fig. 58.2). Try this with different field diaphragm settings until you can see a clear, sharp image (Fig. 59.b).

• Tocenter the sharp image, insert the centering key into the corresponding openings on both sides of the upper portion of the condenser (Fig. 58.1). Move the image into the center of the field of view (Fig. 59.c). Then, open the field diaphragm until the image fills almost the entire im-age field. The black edges of the image should have the same distance from the outer edge of the field of view all the way around. If this is not the case, center the image again using the centering screws. Use the condenser height adjustment to bring the edges into focus. Open the field diaphragm until the image fills the entire field of view and the black edges have completely disappeared from the field of view (Fig. 59.d).

Note:

You can find additional information regarding the correct settings for Koehler illumination in Leica Science Lab.Link:http://www.leica-microsystems.com/science-lab/

Fig. 58: Condenser centering1 Centering holes2 Height adjustment3 Prisms and phase ring centering4 Clamping screw

2 1

3

2 1

3

4

a b

c d

Fig. 59: Koehler illuminationa Illuminated field diaphragm not focused, not centered b Illuminated field diaphragm focused, but not centered c Illuminated field diaphragm focused and centered Diameter is too small, however d Fielddiameter(light)=Fielddiameter(view)(Koehlerillumination)

37

7. Index

38

Symbole

3-plate stages 17

A

Adjusting the illuminated field diaphragm 33Ambient conditions 12Analyzer 21, 36Analyzer slot 8Aperture diaphragm 8, 11

C

Camera 9, 10Centering hole 25Centering procedure for phase contrast 35Checking the phase contrast rings 34Colour filter 8Condenser 11Condenser base 24Condenser centering 35, 37Condenser clamp 25Condenser head 24, 29Condenser height adjustment 11Condenser height stop 11Condenser prisms 24Condensers 23, 24, 28Connection for fixed transmitted light

illuminator arm 31Cover for fluorescent turret disk 8

D

DIC disk 15DIC module 15DIC objective prism disk 9, 10DIC objective prisms 15

E

EXT1-EXT6 sockets 22Eyepieces 8, 10, 18Eyepiece tubes 8, 10

7. Index

F

Field diaphragm 8Fixed micromanipulation stage 17Fixed stage 17Fixed transmitted light illuminator arm 11Fluorescence illuminator 19Focusing telescope 34

I

IC condenser prisms 27IC prism disk 15IC prisms 15Identification sheet 18, 20Illuminator 19Incident light reflector turret 20Incident light turret disk 8, 10Inserts 17Installation location 12

K

Koehler illumination 37

L

Lamp cable connection 31LAS software 18, 26LED lamp housing 10, 19Leica CTR electronics box 31, 32Leica EL6000 19Leica Science Lab 35, 37Light guide 19Light ring 25, 26, 35Light ring slide 11Light source 19

M

Mechanical stage 17

O

Objective prism slide 15Objectives 8, 10, 18Objective turret 8, 10

P

Packaging material 12Parfocality adjustment 18Phase ring 24, 35Polarizer 8, 11, 21, 29, 36

R

Reflector cube 20

S

Safety Notes 6Scanning stage 17Service port 9Setting the motorized polarizer 36Side-port 8, 9Specimen stage 8, 10, 16Stop lever for condenser height adjustment 23Symbols 4

T

Technical data 6Tiltable transmitted light illuminator arm 11Top-port 10Transmitted light column 23Transmitted light filter 11Transmitted light illumination column 23Transmitted light illuminator arm 11, 22Transmitted light lamp housing 11, 30Transmitted light lamp housing receptacle 8Tube 8, 10

W

Weight 13Wiring 31Wiring in the lamp housing 30

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