nstda · research directions based on consultations from industry, government and nstda r&d and...

NSTDATechnology GuideFor SEED INDUSTRY

NSTDA Technology Guide For “SEED INDUSTRY”B NSTDA NSTDA NSTDA BB

Germplasm Bank - Collection, evaluation - Germplasm database - Release

PepperKU (2,827 acc.)

TomatoKKU (1,388 acc.)

Germplasm Bank

CornKKU (Waxy corn 506 acc.)KU (Field corn 1,229 acc.)

Thailand Seed ClusterThailand Seed Cluster was established in 2006 by National Science and Technology Development Agency(NSTDA) in collaboration with more than 75 government and private sectors. The goal of Thailand Seed Cluster is to increase the value of Thai seeds exports and increase research in the private sectors to develop new products in “Thailand brands”.

Research directions based on consultations from industry, government and NSTDA

R&D and TechnologyTransfer - Breeding technology - Diagnostic technology - Smart green house - Seed technology

TechnologyMolecular marker

Diseases screeningDouble haploidEmbryo rescue

Green houce

TechnologyTechnology

CornResistant to

DM and NCLB

CucumberResistant to DM

and visurs

TomatoResistant toTospovirus,

Begomovirusand BW

ChiliMale sterileresistant toanthracnose

and virus

MelonResistant to DM

and bacteria

CucurbitRMUTL

(cucumber 726, pumpkin 415)

CucurbitBiobank

NSTDA Technology Guide For “SEED INDUSTRY” 1Technology Guide For “SEED INDUSTRY”Technology Guide For “SEED INDUSTRY” 11Technology Guide For “SEED INDUSTRY”Technology Guide For “SEED INDUSTRY”

International Collaboration - International field trial - Business matching - R&D - Exchange researchers

Human Resource Devleopment

- Plant Breeder and Seed technologies

- Training/workshop/seminar

For further information, please contact: RDI Management for National Strategic and Network (RNS), Research& Development Management, National Science and Technology Development Agency (NSTDA), Miss Sasiwimon Boonanunt E-mail: [email protected] Tel. +66 2 117 647

NSTDA Technology Guide For “SEED INDUSTRY”2 NSTDA NSTDA NSTDA 22

Breeding ProductPlant breeding for Seed industries and Food Pharmaceutical Industries uses sources of germplasm, science and technology including molecular marker, plant disease screening, technique, embryo rescue, and double haploid.

Highlight productChili Breeding for High Capsaicin

Capsaicin 0 - 50,000 SHU

Red-moon EsanPungency: 30, 000 SHU

Segment: Dry chili

Yodson Khem 80 Pungency: 70,000 SHUSegment: Dry chili and

Pharmaceutical industry

Akkhanee Piroth Pungency: 500,000 SHU

Segment: Pharmaceutical industry

Pech ModindangPungency: 300,000 SHU


Yokkaow ModindangPungency: 100,000 SHU

Segment: Fresh consumption

Red-sun Esan Pungency: 30,000 SHU.

Segment: Dry chili

Capsaicin 50,000 - 100,000 SHU

Capsaicin >100,000 SHU

Yokkaow Modindang

Yellow moonPungency: 200,000 SHU


Yokkeaw ModindangPungency: 100,000 SHU


Pretty hotPungency: 70,000 SHU


Bushy hotPungency: 70,000 SHU


Huay-sithonkanrapruek Pungency: 30,000 SHU.

Segment: Dry chili

Bushy hot


This product is available for licensing. For further information, please contact Technology Licensing Offi ce (TLO) of Khon Kaen University. (https://eng.kku.ac.th) Miss. Chindaporn Pholsungnoen Tel.+66 4320 2733, 08 6451 4455, E-mail [email protected]

Cherry tomato breeding for good quality of consumption

Poungthong• Fruit 10 g.• Yield 2-3 kg./plant• Sweetness 7 %brix

Cherry pear• Fruit 13 g.• Yield 2 kg./plant• Sweetness 10 %brix

Nilmanee• Fruit 10 g.• Yield 2-3 kg./plant• Sweetness 10 %brix

Blackcherry Khamkean• Fruit 10 g.• Yield 2-3 kg./plant• Sweetness 8.2 %brix

Orange KKU• Fruit 4 g.• Yield 1 kg./plant• Sweetness 8-10 %brix

Golden princess• Fruit 6 g.• Yield 2-3 kg./plant• Sweetness 8.2 %brix

Shiny Queen• Fruit 7 g.• Yield 2-3 kg./plant• Sweetness 7-8 %brix

Lyco-red• Fruit 10 g.• Yield 2-3 kg./plant• Sweetness 7 %brix

Cherry plum• Fruit 9 g.• Yield 1.5 kg./plant• Sweetness 8 %brix


Cherry tomato resistant to Tomato Yellow Leaf Curl Virus (TYLCV)

This product is available for licensing. For further information, please contact Technology Licensing Office (TLO) of National Science and Technology Development Agency (NSTDA) E-mail: [email protected] / [email protected]

PC12-20

FD12-3 (A8-3) FD12-LK (A8-LK)

FD12-4 (A8-4) PC6 (A11)

FD4 (A4)

FD1-2 PC3(A9)


Corn breeding for high quality and disease resistance

This product is available for licensing. For further information, please contact Technology Licensing Offi ce (TLO) of Khon Kaen University. (https://eng.kku.ac.th) Miss. Chindaporn Pholsungnoen Tel.+66 4320 2733, 08 6451 4455, E-mail [email protected]

Purple sweet waxy corn“KGW#1”• High anthocyanin• High 177 cm.• Ear length 17 cm.• Maturity 69 day• Hybrid

Purple Tein TB1/TB3• Small-ear, 2 ear/plant• High 160 cm.• Ear length 12.5 cm.• Maturity 65 day• Hybrid

KNW• Small-ear, 2-3 ear/plant• High 117 cm.• Ear length 17 cm.• Maturity 63 day• Hybrid

KND• High anthocyanin• High 170-180 cm.• Ear length 17-18 cm.• Maturity 60-65 day• OP

Samlee Esan• High 170-200 cm.• Ear length 17-20 cm.• Maturity 60-65 day• OP

Purple sweet waxy corn“KGW#2”• High anthocyanin• High 177 cm.• Ear length 17 cm.• Maturity 69 day• Hybrid

Tein whan• Small-ear, 2-3 ear/plant• High 145-160 cm.• Ear length 14-16 cm.• Maturity 55-60 day• Hybrid

Tri Tein• Small-ear, 2-3 ear/plant• High 190 cm.• Ear length 12 cm.• Maturity 60 day• OP

Bi-color• High 165-175 cm.• Ear length 12-16 cm.• Maturity 65-70 day• OP

DK• Big ear, stalk strength• High 170-180 cm.• Ear length 18-20 cm.• Maturity 70-75 day• OP


Development of Plant Diagnostics for Agriculture of the NationAgriculture has significantly contributed to Thailand’s economic growth. Pests and pathogens pose serious threats to crop production by reducing both quantity and quality of the products. An ability to detect and identify plant pathogens is essential to epidemiological study, disease management, selective breeding programs for pathogen-resistant plants, and certification of disease-free seeds for import and export. Although anti-bodies and diagnostic test kits for plant pathogens are commercially available, they remain extremely expensive and often not specific to the local pathogen species. Development of a rapid, specific, accurate and cost effective diagnosis system is therefore necessary for crop production in Thailand.

Monoclonal Antibody Production and Application Research Team specializes in monoclonal antibody production and immunological assay development, focusing mainly on diagnosis for agricultural relevance. A catalog of mouse monoclonal antibodies and rabbit polyclonal antibodies has been successfully established for detection of plant pathogens that cause serious plant diseases in economically important plants such as tomato, pepper and cucurbits. By applying our available antibodies, various immunological assays such as enzyme-linked immunosorbent assays, immunochromatographic strip tests and immunomagnetic separation for efficient detection of the target pathogens have been successfully developed. Several antibodies specific to plant pathogens are continuously purchased by government sectors, seed companies and diagnostic companies worldwide. An immunochromatographic strip test for detection of a devastating disease in cucurbits developed by our research team has already been licensed to a private company.


1. Antibodies for Detection of Plant Pathogens

A catalog of mouse monoclonal antibodies and rabbit polyclonal antibodies is available for detection of plant pathogens that cause serious plant diseases in tomato, pepper and cucurbits such as Begomovirus, Tospovirus, Potyvirus, Polerovirus, Tobamovirus and Acidovorax avenae subsp. citrulli. These available antibodies can be used in enzyme-linked immunosorbent assays for an efficient detection of the plant diseases.

Further information about commercialization and enquiries on licensing, please contact BIOTEC Business Development Division. (www.biotec.or.th/en/ +66 2564 6700)

Highlight Researches


2. IC Strip Test for Detection of Bacterial Fruit Blotch Disease in Cucurbits.

The immunochromatographic (IC) strip test has been developed for rapid detection of Acidovorax avenae subsp. citrulli. (Aac) in cucurbits such as watermelon, melon, squash, cantaloupe and cucumber. The IC strip test provides seed exporters and government sectors with an accurate, cost-effective, convenient and very rapid for the detection of Aac within 5 minutes. The developed IC strip test is very useful for epidemiological studies, disease management and screening of disease-free seeds for quality control.

This IC strip test for Aac has already been licensed to Parwel International Co., Ltd. (www.parwelinter.co.th +66 2434 3671-3).


3. IC Strip Test for Detection of Potyviruses

The immunochromatographic (IC) strip test has been developed for rapid and broad-spectrum detection of Potyviruses such as Zucchini yellow mosaic virus, Papaya ringspot virus Type W, Watermelon mosaic virus-2, Papaya ringspot virus Type P, Chilli veinal mottle virus, Potato virus Y, Tobacco etch virus and Bean common mosaic virus. The IC strip test is specific, sensitive, accurate, cost-effective, convenient and very rapid for the detection of Potyviruses within five minutes.

Further information about commercialization and enquiries on licensing, please contact BIOTEC Business Development Division (www.biotec.or.th/en/+66 2564 6700)


4. IC Strip Test for Detection of Watermelon mosaic virus-2

The immunochromatographic (IC) strip test has been developed for spe-cific detection of Watermelon mosaic virus-2. The IC strip test is specific, sensitive, accurate, cost-effective, convenient and very rapid for the de-tection of Watermelon mosaic virus-2 within five minutes. The developed IC strip test is very useful for epidemiological studies, disease management, selective breeding programs for virus-resistant plants and screening of disease-free seeds for quality control.



5. IC strip test for multiplex detection of three plant pathogens in cucurbits

The IC strip test has been developed for multiplex detection of three plant pathogens in cucurbits including Potyvirus, Watermelon mosaic virus-2 (WMV-2) and Acidovorax avenae subsp. citrulli. (Aac). This strip test is specific, sensitive, accurate, cost effective and very convenient giving out the result within five minutes.



6. Enrichment-IMS-plating method for detection of live Acidovorax avenae subsp. citrulli. in cucurbit seeds

In collaboration between BIOTEC and CHIA TAI, an enrichment-immunomagnetic separation (IMS) - plating method has been developed for detection of live Acidovorax avenae subsp. citrulli (Aac) in cucurbit seeds. This method consists of three major steps:

(i) enrichment protocol to increase Aac population in seeds, (ii) immunomagnetic separation using anti-Aac immunomagnetic beads (iii) isolation of Aac on an effective semi-selective medium. The enrichment- IMS-plating method detects Aac in the 0.1% infected seed lot (1 infected seed in 1,000 healthy seeds) with a low level of contamination at 100 CFU/seed lot. The new method showed 100% correlation with the standard seedling grow out (SGO) test in detection of Aac in watermelon seed samples from both inoculated and commercial seed lots. Furthermore, this method requires only 8 days to obtain the results in contrast to 28 days for the SGO test. The enrichment-IMS-plating method is sensitive and accurate with a shorter assay time compared to the standard SGO test.



7. Multiplex RT-PCR-ELISA system for tospovirus species identi-fication using universal primers and species-specific probes

A multiplex RT-PCR-ELISA system has been developed for the identification of four distinct species of tospovirus commonly found in tomato, pepper and cucurbits production fields in Thailand, namely Capsicum chlorosis virus (CaCV), Melon yellow spot virus (MYSV), Tomato necrotic ringspot virus (TNRV), and Watermelon silver mottle virus (WSMOV). The system is based on the use of only one universal primer pair for amplifying four different tospovirus nucleocapsid (N) genes simultaneously in one RT-PCR reaction, followed by detection and differentiation of N gene-derived PCR products by four parallel microwell-captured specific probe hybridizations and ELISA. The multiplex RT-PCR-ELISA system is very effective at tospovirus detection and identification in field-collected plant samples, demonstrating superior specificity, sensitivity and ease of sample processing to a conventional RT-PCR. The system could be used as a high-throughput method for the identification of different tospovirus species.



The National Omics Center (NOC) is equipped with a number of state-of-the-art sequencing platforms that facilitate rapid single nucleotide polymorphism (SNP) marker discovery and genotyping for downstream applications in the seed industry such as hybrid seed purity test, cultivar identification and marker-trait association analyses.

Hybrid seed purity test & cultivar identification: We have developed an affordable approach that can simultaneously identify SNP markers in multiple plant genomes, enabling the development of fully customized SNP marker sets for F1 hybrid seed purity test as well as cultivar identification. Species that we have worked with includes cucumber, melon, chilli pepper, tomato, watermelon and bitter gourd. The ubiquitous nature of SNP markers makes them extremely useful for differentiating

SNP marker discovery and genotyping services for hybrid seed purity test, cultivar identifi cation and marker-trait association analyses


closely related pure lines that are often indistinguishable based on morphology or microsatellites/simple sequence repeat (SSR) markers. Our seed purity testing service has a fast turn-around time – it is carried out on the newest model of an u l tra high-throughput Intelliqube® SNP genotyping platform that can handle thousands of samples in just a few days.

Marker-trait association analyses: Marker-assisted selection can really expedite the process of developing elite cultivars, especially for crop species with a long life cycle. We provide a complete one-stop service for the identification of quantitative trait loci (QTL) and the development of SNP markers linked to agronomic traits of interest (e.g, disease resistance, yield, sex ratio, fruit size, bitterness) that can be used in breeding programs. Markers associated with traits of interest can be identified from either segregation populations derived from bi-parental crosses or a large collection of available germplasm. We also provide a multiplexed SNP genotyping service on the MassArray® system that allows simultaneous genotyping of 30-40 SNPs (associated with multiple desirable traits) in one reaction.

For further information, please contact The National Omics Center (NOC) E-mail: [email protected] Tel. +66 2 564 6700 ext.3537


National Science and Technology Development Agency (NSTDA)

111 Thailand Science Park (TSP), Phahonyothin Road,Khlong Nueng, Khlong Luang, Pathum Thani 12120, Thailand Tel. +66 2564 7000 E-mail: [email protected]

nstda · research directions based on consultations from industry, government and nstda r&d and...

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