MUTAGENICITY EVALUATION OF VEGETABLES GROWN INDIESEL EXHAUST CONTAMINATED SOIL USING THE WING

SOMATIC MUTATION AND RECOMBINATION TEST INDROSOPHILA MELANOGASTER

JANPEN SAI(SITPITAK

With comPlirnents, - of

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A THESIS SUBMITTED IN PARTIAL FT]LFILLMENT OFTHE REQTIIREMENTS F'OR THE DEGREE OF

MASTER OF'SCIENCE(FOOD Ar{D NLnRTTTONAL TOXICOLOG9

FACI]LTY OF GRADUATE STT]DIESMAHIDOL T]NIVERSITY

1998ISBN 974-661-209-3

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3836203 NUTX/M : MAJOR : FOOD AND NUTzuTIONAL TOXICOLOGY ;

M.Sc. ( FOOD AND NUTzuTIONAL TOXICOLOGY)KEY WORD : MUTAGENICITY/ DIESEL EXHAUST/ PLANT

CONTAMINATIONJANPEN SAKSITPITAK : MUTAGENICITY EVALUATION OF

VEGETABLES GROWN IN DIESEL EXHAUST CONTAMINATED SOIL USINGTHE WING SOMATIC MUTATION AND RECOMBINATION TEST INDROSOPHILA MELANOGASTER. THESIS ADVISOR : KAEWKANGSADALAMPAI, Ph. D., ANADI NITITHAMYONG , Ph. D. WANNEEKUSAMRAN, Ph. D. 77 p. ISBN 974-661-209-3

Larvae of the improved high bioactivation (IHB) cross Drosophilamelanogaster (ORR;ltl/TM3, Ser females mated with mwh males) were fed withmedium containing hexane extract of the edible portion of five vegetables grown inthree drfl'erent soil treatments (controi soil, soil treated with I g of diesel exhausL&g

soil and soil treated with 10 g ofdiesel exhaust/kg soil) for 48 h. The wing hairs ofthesurviving flies were analyzed for the frequency and size of single and twin spots. Itwas found that clone induction frequency ofthe wing hairs of flies treated with hexane

extract of leaves of sacred basil and green kuang futsoi grown in contaminated soilwas not statistically different from that of vegetables grown in control soil. Clearpositive results were obtained when larvae were brought up in the medium containinghexane extracts of lettuce and of water spinach grown in contaminated soils.

Interestingly, the extracts of multiply onion, both grown in the treated and untreated

soils, induced mutation in the wing spot test. It was concluded that some plants grownin diesel exhaust contaminated soil provoked mutagenic responses while some

showed negative results.

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