Automated Platform for Live Cell Imaging

MOTORIZED INVERTED SYSTEM MICROSCOPE

IX81IX2 SERIES

UNIVERSAL INFINITY SYSTEM

Microscope body Revolving nosepiece Sextuple motorized with objective lens retraction in PC mode, simple waterproof mechanism incorporated

Focus 9mm stroke (from stage surface, 7mm upward and 2mm downward), fine/coarse switchable focusingknobs (minimum graduation: 0.1µm), objective lens escape/return buttons and return to memoryposition buttons (each side of microscope frame)

Primary image port Lower port with motorized 2 step optical path selection (standard — left side port: S1F 100% or S8F80%, or optional — lower back port selectable) Upper port (special order: right side port or upper backport selectable, 2-step light path selection), Bottom port (option — motorized or manual selectable)

Frontal operation Light path selector button, Transmitted light intensity control buttons and light ON/OFF switch button,Fine/Coarse focus selector button, TTL Pulse control switch (auxiliary) buttons

Transmitted light illuminator 100W transmitted IX2-ILL100 Pillar tilt mechanism (30° inclination angle, with shock decreasing mechanism), light illumination pillar Condenser holder (with 50mm stroke, swing-in/out mechanism), Field iris diaphragm adjustable,

4 filter holders (ø45mm, t=6mm or less) External power supply unit IX2-UCB Auto voltage selector (100V / 200V), RS232C interface for PC operation, IX2-BSW driver software

Observation tube Tilting binocular tube U-TBI90 35-85° continuous angle adjustable (eyepoint height range: 406mm-471mm), interpupillary distanceadjustable between 50-76mm, diopter adjustment function, erect image, F.N. 22

Binocular tube U-BI90CT Built-in focusing telescope, interpupillary distance adjustable 50-76mm, diopter adjustment function, F.N. 22

U-BI90 Interpupillary distance adjustable 50-76mm, diopter adjustment function, F.N. 22Trinocular tube TR30H+IX-ATU 3 step optical path selectable (observation: straight port = 100:0, 20:80, 0:100),

interpupillary distance adjustable 50-76mm, diopter adjustment function, F.N. 22Stage Cross stage with flexible right handle IX2-SFR 50mm(X) x 50mm(Y) stroke, stage insert plate exchangeable (ø110mm)

Cross stage with short left handle IX-SVL-2 50mm(X) x 43mm(Y) stroke, stage insert plate exchangeable (ø110mm)Plain stage IX2-SP 232mm(X) x 240mm(Y) stage size, stage insert plate exchangeable (ø110mm)

IX-MVR Mechanical stage to be used with IX2-SP, 130mm(X) x 85mm(Y) strokeNarrow plain stage IX2-KSP 160mm(X) x 240mm(Y) stage size, stage insert plate exchangeable (ø110mm)

CK40-MVR Mechanical stage to be used with IX2-KSP, 120mm(X) x 78mm(Y) strokeGliding stage IX2-GS Upper circular stage 360° rotatable, 20mm(X/Y) travel

Condenser Motorized long working IX2-LWUCDA Motorized turret with 6 position slots for optical devices (3 positions each for ø30mm and ø38mm), distance universal aperture iris diaphragm adjustable, N.A. 0.55 / W.D. 27mmLong working distance Relief Contrast IX2-MLWCD 4 positions for optical devices (for ø50mm, Relief Contrast optical devices rotatable),

aperture iris diaphragm adjustable, N.A. 0.5 / W.D. 45mmUltra long working distance IX-ULWCD 4 positions for optical devices (for ø29mm), aperture iris diaphragm adjustable, N.A. 0.3 / W.D. 73mmWater immersion DIC IX2-DICD+IX2-TLW Single position for optical device (include two optical device holders), 40 injection pipette or

electrode insertion angle, aperture iris diaphragm adjustable, N.A. 0.9 / W.D. 3.7mmEyepiece WH10x High eyepoint, F.N. 22

WH10x-H High eyepoint, diopter adjustment function, F.N. 22Reflected light Fluorescence illuminator IX2-RFAL L-shaped design with exchangeable F.S. and A.S. modules, fluorescence unit two filter holder sliders (2 positions, ø32mm, t=6mm or less)

IX2-RFA Straight design with field iris diaphragm, filter holder slider (2 positions, ø32mm, t=6mm or less)Fluorescence cube turret IX2-RFACA Motorized turret with 6 positions, built-in shutterLight source 100W HBO lamp housing and HBO transformer, or 75WXBO lamp housing and XBO transformer

667

*406

~47

1

*243~391

290

135

299

394

IX81 dimensions (unit: mm)

IX81 specifications

See manual

151.

4

212

216

12515 310

332 (depth)

OFFON

SW2

SW1

HS

RS232C

ERR

NP

Z/AFCDTTLFW3FW2FW1ASRSHTMU

RMT

IX-UBC

30°

64.348

.638

.0

91.5

50.0

75.54.0 70.0

35.582.3

28.354.0

146

7°180

105

OB

IX2-UCB dimensions (unit: mm) U-FH dimensions (unit: mm)

U-HSTR2 dimensions (unit: mm)

Weight: 26.0kg Power consumption: 350VA * Lengths with an asterisk (*) vary according to interpupilllary distance.

Weight: 5.0kg Rated voltage: 350VA Weight: 0.8kg

Specifications are subject to change without any obligation on the part of the manufacturer.

ISO9001Certification

Design and production

adheres to ISO9001

international quality standard.ISO 9001

Accredited by the Dutch Council for

Accreditation

ISO14001Design and production at the Olympus Optical Co. Ltd. Ina Plant conforms with

ISO14001 specifications for environmental management systems.

Certification

ISO 14001008

U K A SENVIRONMENTAL

MANAGEMENT

The IX81 is Olympus' most advanced motorized microscope, providing high standards of

observation, measurement and functional operation. A V-shaped optical path, improved

fluorescence illuminators and further expanded UIS optics provide excellent performance for

research applications. A modular system of motorized accessories allows for complete

customization to individual research needs. Multi-dimensional analyses can be performed

automatically or even remotely via computer control. Additionally, the IX81 features numerous input

and output ports allowing the use of several light sources and detectors.

The IX81 is the optimum imaging platform for research and automation.

Advanced, fully motorized system for

superior performance.

1 2

*Note:UIS optics: The original Olympus infinity-corrected optical system. This system expresses the superb Olympus optical technology, provides high resolution,high contrast images and the flexibility to meet current and future applications.

43

Motorized universal condenser/IX2-LWUCDA6 turret positions allow brightfield, phase contrast and DIC observations. Also accepts 32mm filters.

Motorized shutter/IX2-SHACan be mounted in both transmitted andreflected light paths.

Motorized filter wheel/U-FWR and U-FWO6 filter positions (32mm or 25mm diameter).

Microscope front panelEasy to use buttons allow selection of light path, focus fine/coarse switchover, light intensity and lamp on/off control. Two auxiliary buttons can be customprogrammed. Includes LED lamp intensity meter.

A selection of independent motorized units forconvenient operation.All major microscope functions can be motorized, including focus,illumination, objectives and optical path selection. The motorizedunits for the IX81 can be operated via a remote handset or buttonson the microscope body. All controls are close to the operator'shand, maximizing user comfort while minimizing needed benchspace.

Computer control for routine and research applications.All motorized functions are also controllable via computer. Imagingsoftware can be used to facilitate microscope operations rangingfrom routine to complex. Buttons on the microscope body and

remote handset canbe programmed forcustom operation.

A flexible motorized platform for automated system solutions.

Motorized sextuple revolving nosepieceIncluded with microscope frame.

Internal motorized focus drive0.01µm minimum step size.

Motorized fluorescence cube turret/IX2-RFACA6 fluorescence filter cube positions.

Light path selector

Transmitted light ON/OFF

Transmitted light intensity control

Auxiliary buttons

Fine/Coarse

Handset/U-HSTR2A remote handset controls allmotorized functions via a convenientand programmable interface.

System controller/IX2-UCBAll motorized units are powered bythis external system controller.Included is an RS232C connectionfor PC commands and expansionslots for future system upgrades.

Focus handle/U-FHThe remote focus handle duplicatesthe feel and function of themicroscope's focus knobs. Additionalcontrols include fine/coarse focusselection, lamp on/off, shutteropen/close, and camera vs. visualobservation.

Motorized bottom port unit with C-mount/IX2-TVRAC

Objective escape and zero-return buttonsMoves objective to lower focus limit. Allows setting of default focus position.* Equipped on each side of microscope frame.

Escape button

Return to memory position button

U-FWR U-FWO

LEFT SIDE PORT (Primary Image)

For film or CCD camera systems. Double port tubeallows the attachment of two cameras (both primaryimages). Also accepts Olympus confocal scan units.

RIGHT SIDE PORT (Primary Image)

Used for connecting an additional light source orCCD camera.

BOTTOM PORT(Primary Image)

Well suited for extremely sensitive low light levelimaging.

UPPER BACK PORT(Primary Image)Used for connecting an additionallight source or CCD camera.

LOWER BACK PORT(Primary Image)For the attachment of largebodied items such as cooledCCD cameras or confocal scanunits.

BINOCULAR PORTCCD camera mount via sideport intermediate tube.

GX-SPU side portintermediatetube

65

Primary images areobtainable from all 4 portssimultaneously

D

C

B

A B C D

A

Unique two-tiered multi-port design offers input/output system flexibility andapplication support

The IX frame begins with 6 input/output ports for a wide variety of lightsources or cameras. The unique Olympus design allows up to four portsto have simultaneous access to the primary image, without relay lenses,providing cameras with the highest quality image, free of aberrations.Two-tiered multi-port design maximizes flexibility while maintaining a299mm low stage height. The compact and rigid frame requires aminimum amount of bench space, easily accommodates externalequipment and remains stable during extended observations. Over 100mm of free space is available at the left side port between the frame and the primary image, easily allowing for adaptation ofinstrumentation for individual research requirements.

IX2-RSPCRight side port attachment with C-mount

IX2-TVR bottom port adapter+CCD camera

U-DPCAD double port tube

D

394mm in depth

102mm of

free space

299mm

low stage height

UPPER BACK PORT

BINOCULAR PORT

LOWER BACK PORT

LEFT SIDE PORT

RIGHT SIDE PORT

BOTTOM PORT

A

C

B

GFP, CFP, YFP Filter CubesFluorescence performance has been improved with high efficiencycoatings minimizing signal crossover.Filter sets are optimized for

specific fluorochromes such asGFP, CFP and YFP. All Olympusfilter cube housings incorporateanti-reflective materials thatminimize stray light .(Available autumn 2002)

High quality images of living cells

87

Fully automatic confocal system to meeta wide range of needsThe Fluoview confocal scan unit can be attached to the back port, leaving ample spacefor manipulators and electrophysiological equipment. Combining the IX81 with theFluoview FV500 provides a fully capable, multi-channel, motorized system. Bothconfocal and fluorescence observation equipment can be attached simultaneously, andport changing can be performed automatically.*Special microscope body available for Fluoview.

Improvements in the performance of the UIS optical system haveresulted in sharper, clearer images. Two reflected light fluorescenceilluminators are now available. • An L-shaped illuminator includes centerable aperture and fieldstops with convenient controls close to the user. L-shape alsopermits the mounting of a large bodied camera to the back port. • A straight illuminator designed for maximum intensity provides thebrightest possible images.

A variety of fluorescence filter cubes are available. Up to 6 cubescan be installed in the motorized turret, with easy selection via theremote handset. An optional DIC filter cube incorporates an analyzer and allows automatic switching between DIC observation andfluorescence.

The IX2-LWUCDA motorized universal condenser enables standardbrightfield, phase contrast and DIC observations. Three series ofDIC prisms are available for optimum contrast enhancement with awide variety of specimens. Standard prisms are best for most

observations, while HighContrast (HC) prisms providemaximum contrast for thinspecimens, and HighResolution (HR) prismsprovide maximum detail withthicker specimens.

High quality images meet the demands of advanced analytical applications.

Computer controlled multi-color andtime-lapse applicationsMotorized precise internal Z-drive, objective nosepiece and accessories allow multi-dimensional, multi-spectral imaging via a computer. A thermally and structurally stableframe design combine to create an ideal system for long term time-lapse anddeconvolution observations.

Relief Contrast Condenser provides long working distance (W.D.45mm) for injector access and a high numerical aperture (N.A. 0.5)for excellent images with plastic dishes. With a long workingdistance and high N.A. Phase contrast and DIC are also possible.

Unique Relief Contrastobjectives feature rotatablemodulators for consistantshading for all magnifications.

New fluorescence accessoriesfor improved sensitivity

Optimum images forMicroinjection

4500.0

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0.8

0.9

1.0

470

BP475

BA515-550BA510-550

490 510 530 550 570

T％�

Wavelength (nm)

Long working distance objectives/LUCPLFL40xAccommodating vessel thicknesses from 0-2mm with a correctioncollar mechanism that maintains objective focus during adjustment.

Correcting for different vessels isfast and simple. Lens design for340nm transmission allows theseobjectives to be used for UVfluorescence applications suchas FURA-2.

Rectangular field stop/IX2-RFSSPinhole field stop/IX2-RFSPOTFlexible field stop options IX2-RFSS rectangular field stop module orIX2-RFSPOT pinhole field stop module can be mounted into the

L-shaped illuminator for improvedillumination control duringfluorescence imaging orphotobleaching experiments.(Available autumn 2002)

The new IBA cube has a capability tomaximize defection of GFP emission.

IX-ATUIntermediate tube

WH10xWH10x-HEyepiece

U-BI90Binocular tube

U-BI90CTBinocular tube with centering telescope

GX-SPUSide port intermediate tube

U-TVZZoom video port tube

U-TV1x-2Primary image video port tube

U-TV0.5xC mountvideo port tube with 0.5x lens

U-TV0.5xCC mountvideo port tube with 0.5x lens

U-TV0.35xCC mountvideo port tube with 0.35x lens

U-TV0.25xCC mountvideo port tube with 0.25x lens

IX-SPTSingle port tube

PE Photo eyepiece

U-PMTVCC mountvideo port tube with 0.3x lens

U-PMTVVideo port tube with 0.3x lens

U-PMTV1xVideo port tube

U-CMAD3C mountadapter

U-BMADBayonetmountadapter

U-IMADIkegamimountadapter

U-FMTF mount adapter

U-CMTC mount adapter

IX-TVADPrimary imagevideo port tube

U-TMADT mount adapter

TDO-100Power supply unit

U-SMADSonymountadapter

IX-SVL-2Cross stage with short left handle

IX2-KSPNarrow plain stage

IX2-GCP*1Insert plate

IX-HOPPetri dish holder

IX-HOTTerasaki plate holder

IX-HOSSlide glass holder

U-FMPMechanical stage

U-CLACollector lens adjustment

U-IFFHFocus handleInterface

U-FHFocus handle

PC(IX2-BSW)

U-HSTR2Handset

U-LH75XEAPO75W xenon apolamp housing

U-DULHADual lamp housing attachment

Power supply unit

Power supply unit

U-LH100HG100W mercurylamp housingU-LH100HGAPO100W mercury apolamp housing

U-LH100-3100W halogen lamp housing

U-LH100L-3100W halogen lamp housing with long cable

U-RMTExtension cord

U-FWOMotorized observation filter wheel

U-FWOMotorized observation filter wheel

IX-CP50Insert plate

IX-SCLSlide clip

IX-PP24NUNScale for NUNCTM plate Incubator

Heat plate

Heat plate

U-TR30HTrinocular tube

U-ECAMagnification changer 2x

U-ECA1.6xMagnification changer 1.6x

U-CAMagnification changer 1x, 1.25x, 1.6x, 2x

U-EPA2Eyepoint adjuster

IX-SDOSide by side observation attachment

U-KPAIntermediate attachment for simple polarising observation

CK40-MVRMechanical stage

IX-MVRMechanical stage

CK2-SSSide stage

U-DICTSShift DIC slider fortransmitted lightU-DICTHRHigh resolution DICslider for transmitted lightU-DICTHCHigh contrast DIC sliderfor transmitted light

IX2-RFAEVA*2Total internal reflection fluorescence illuminator

FV5-FUR*2Fiber unit

IX2-PRBP1*2100% prism unit for lower back portIX2-PRBP8*220/80% prism unit for lower back port

FV5-LAAR*2Argon ion laser

IX-CICritical illuminator

IX-HLSH100100W halogen lamp housing centerable

IX2-ILL100Transmitted illuminator

45LBDIF45ND2545ND643IF550W45Filters

IX-ULWCDUltra long working distance universal condenser

IX2-MDICTAnalyzer unit for transmitted light

Mirror unit

IX-ADUCDFocusable adapter

U-FWRMotorized reflected filter wheel

U-UCD88 position universal condenser

U-TLOOil top lens

U-TLDDry top lens

IX2-MLWPOPolarizer attachment

U-TLOOil top lens

U-TLDDry top lens

IX2-MLWCDMid long working distance condenser

IX2-DICDWater immersion DIC condenser

IX2-TLWWater top lens

U-TBI90Tilting binocular tube

U-DPCADDual port tube with C-mounts

IX2-SFRCross stage with flexible right handle

IX2-RFALL shape fluorescence illuminator

IX2-RFAFluorescence illuminator 32ND6-2

32ND12-232ND25-232ND50-232FR80ND filters

IX2-RFACAMotorized fluorescence cube turret

IX2-RFACFluorescence turret

IX2-CA2x*22x lens unit

IX-RSPC*2Right side port attachment with C-mount

IX2-SPPlain stage

IX2-TVRAC*2Motorized bottom port unit with C-mount

IX2-LBPC*2Lower back port unitwith C mount unit

IX2-FPFrame plate adapter

IX2-ANAnalyzer

IX2-PRS1*220/80% prism unit for side portIX2-PRS8*2100% prism unit for side port

IX2-SPTSingle port tube

IX2-UCBSystem controller

Optical devices

Optical devices

Optical devices

IX-LWPOPolarizer attachment IX2-LWUCD

Long working distance universal condenser

IX81FIX81 frame without prism

A

B

D

D

A

E

B

C

CD

E

IX2-SHAMotorized shutter

IX-PPMMillimeter scale

IX-CLMT,IX-CLM96Well positioner

IX2-LWUCDAMotorized long working distance universal condenser

IX2-GSGliding stage

IX2-TVR*2Bottom port unit *1 Not available in some areas

*2 To consult with local Olympus subsidiary

Objectives

U-CT30Centering eyepiece

CO2 Incubator

IX2-NPSNosepiece stage

U-MOMonocular tube with eyepiece

A

IX2-RFSSRectangular field stopIX2-RFSPOTPinhole field stop

IX81 SYSTEM DIAGRAM

9 10