MOLD FORM OF FUNGUS

“SUPER FUNGUS”

FRUITING BODY OF PENICILLIUM

FRUITING BODY OF ASPERGILLUS

FRUITING BODY OF ASPERGILLUS

MICROCONIDIA

MACROCONIDIA OF ALTERNARIA

ARTHROSPORES

MACROCONIDIA OF FUSARIUM

MACROCONIDIUM OF M. CANIS

MACROCONIDIUM

HYPHA

MICROCONIDIA

TRICHOPHYTON

COILED HYPHA OF TRICHOPHYTON

SPORANGIUM

MUCOR

YEAST FORM OF FUNGUS

DERMATOPHYTE INFECTIONS

• GENERA– MICROSPORUM,– TRICHOPHYTON– EPIDERMOPHYTON

DERMATOPHYTE INFECTIONS

• LESIONS– HAIR- BROKEN, THICKENED– SKIN- CIRCUMSCRIBED, MAY BE

THICKENED OR ERYTHEMATOSUS

– NAILS- BRITTLE, CHALKY

DERMATOPHYTE INFECTIONS

• ANIMAL SPECIES – DOG- M. CANIS, M. GYPSEUM, T.

MENTAGROPHYTES– CAT-M. CANIS– CATTLE- T. VERRUCOSUM– PIG- M. NANUM– HORSE- T. MENTAGROPHYTES, T.

EQUINUM, M. GYPSEUM, M. CANIS

MICROSPORUM CANIS INFECTION

MICROSPORUM GYPSEUMINFECTION

MICROSPORUM CANIS INFECTION

MICROSPORUM CANIS INFECTION

TRICHOPHYTON MENTAGROPHYTES INFECTION

TRICHOPHYTON MENTAGROPHYTES INFECTION

TRICHOPHYTON INFECTION

TRICHPHYTON

TRICHOPHYTON

TRICHOPHYTON

SCALE/SCAB FROM LESIONTRICHOPHYTON OR DERMATOPHILUS

MICROSPORUM GYPSEUM INFECTION

MICROSPORUM GYPSEUM INFECTION

MICROSPORUM NANUM INFECTION

MICROSPORUM NANUM INFECTION

TRICHOPHYTON VERUCOSSUM

TRICHOPHYTON VERUCOSSUM

TRICHOPHYTON VERUCOSSUM

DERMATOPHYTE INFECTIONS

• DIAGNOSIS– CLINICAL SIGNS– WOOD’S LIGHT- M. CANIS– KOH DIGEST – CULTURE – DTM, FUNGASSAY- TURNS RED – BHICC

RINGWORM OR DERMATOPHYTOSIS

RINGWORM OR DERMATOPHYTOSIS

RINGWORM OR DERMATOPHYTOSIS

DERMATOPHYTE INFECTIONS

• DIAGNOSIS – WOOD’S LIGHT- M. CANIS– KOH DIGEST – CULTURE – DTM, FUNGASSAY- TURNS RED – BHICC

DERMATOPHYTOSISWOOD’S LIGHT POSITIVE

FLUORESCENCE

FLUORESCE

DERMATOPHYTE INFECTIONS

• DIAGNOSIS – WOOD’S LIGHT- M. CANIS– KOH DIGEST – CULTURE – DTM, FUNGASSAY- TURNS RED – BHICC

HYPHA IN KOH DIGEST OF SKIN

HYPHAE IN KOH DIGEST OF SKIN

HYPHA IN KOH DIGEST OF HAIR

DERMATOPHYTE INFECTIONS

• DIAGNOSIS – WOOD’S LIGHT- M. CANIS– KOH DIGEST – CULTURE

•DTM, FUNGASSAY- TURNS RED •BHICC

DERMATOPHYTE INFECTIONS

• DIAGNOSIS- CULTURE– ALCOHOL PREP BEFORE SCRAPING– INOCULATE SURFACE– WATCH FOR RED COLOR WITH WHITE TO TAN

SURFACE GROWTH ON DTM BEFORE 14 DAYS– RED COLOR IS PRESUMPTIVE- DO TAPE MOUNT

TO LOOK FOR HYPHAE, MICRO-/MACROCONIDIA WHICH MAY BE CHARACTERISTIC OF A DERMATOPHYTE

DTM OR FUNGASSSAY MEDIUM

DTM OR FUNGASSAY

POSITIVE CULTURES

NEGATIVE CULTURES

NEGATIVE CULTURES

DTM OR FUNGASSAY

NEGATIVE POSITIVE

DTM OR FUNGASSAY

POSITIVEFALSE

POSITiVE

POSITIVE DTM OR FUNGASSAYTOP OF PLATE

POSITIVE DTM OR FUNGASSAYBACK OF PLATE

POSITIVE DTM OR FUNGASSAY

FALSE POSITIVE DTM OR FUNGASSAY

DTM OR FUNGASSAY

NEGATIVE

POSSIBLEPOSITIVE

DTM OR FUNGASSAYPOOR SPECIMEN

DTM OR FUNGASSAYPOOR SPECIMEN

MACROCONIDIUM OF M. CANIS

MACROCONIDIA MICROSPORUM GYPSEUM

MACROCONIDIA MICROSPORUM NANUM

TAPEMOUNT OF TRICHOPHYTON

HYPHA

MICROCONIDIA

TAPEMOUNT OF TRICHOPHYTON

MACROCONIDIA

HYPHA

MICROCONIDIA

TAPEMOUNT OF TRICHOPHYTON

COILED HYPHA

HUMAN DERMATOPHYTE INFECTION

HUMAN DERMATOPHYTE INFECTION

HUMAN DERMATOPHYTE INFECTION

HUMAN DERMATOPHYTE INFECTION

HUMAN DERMATOPHYTE INFECTION

HUMAN DERMATOPHYTE INFECTION

SKIN INFECTIONS

• DERMATOPHILUS– STREPTOTRICHOSIS

– DIAGNOSIS BY DIRECT EXAMINATION

SKIN INFECTIONS

• DERMATOPHILOSIS- DIAGNOSIS– REMOVE SCABS- ZOONOTIC ORGANISM – PLACE SCABS IN TEST TUBE. – ADD ONLY ENOUGH WATER TO BREAK UP SCAB – THE SLIGHT AMOUNT OF SUPERNATANT SHOULD

BE TURBID AFTER THE SCABS ARE BROKEN DOWN.

– SPREAD DROP OR TWO OF SUPERNATE OVER A 1 X 3 CM AREA OF SLIDE. G. AIR DRY, FIX IN ALCOHOL

SKIN INFECTIONS

• DERMATOPHYOSIS- DIAGNOSIS– STAIN WITH GIEMSA FOR 15-25

MINUTES – LOOK FOR RAILROAD TRACKS UNDER

OIL IMMERSION

MICROSPORUM CANIS INFECTION