milliplex™ map and the endocrine system
DESCRIPTION
The endocrine system is an integrated system of glands and hormones. Consequently, an understanding of how these hormones affect downstream pathways is fundamental in research. This product guide includes a list of EMD Millipore’s available MILLIPLEX MAP endocrine hormone, pituitary, stress and thyroid panels, based on the Luminex xMAP platform. MILLIPLEX MAP enables you to investigate the modulation and expression of dozens of analytes simultaneously, giving you the advantage of speed and sensitivity, and dramatically improving productivity.TRANSCRIPT
Data SheetProduct Selection Guide
MILLIPLEX™ MAP and the Endocrine SystemLuminex® xMAP® Technology
RAT
T3
FSH
ACTH
Leptin
CANINE
HUMAN
RAT
MOUSE
The First Multiplex Panels for the Study of Key Hormones of the Endocrine System
ENDOCRINE SYSTEM
MILLIPLEX MAP and the Endocrine SystemKnowledge of how the hormones of the endocrine system affect downstream pathways is essential to understanding normal homeostasis as well as disease pathogenesis.
The endocrine system is an integrated system of glands and
hormones. Consequently, an understanding of how these
hormones affect downstream pathways is fundamental in
research. This product guide includes a list of Millipore’s
available MILLIPLEX MAP endocrine hormone, pituitary, stress
and thyroid panels, based on the Luminex xMAP platform.
MILLIPLEX MAP enables you to investigate the modulation and
expression of dozens of analytes simultaneously, giving you the
advantage of speed and sensitivity, and dramatically improving
productivity.
Research involving the glands and hormones of the endocrine system play a significant role in achieving a deeper understanding of disease states such as:• Metabolic Syndrome
• Type 1 and 2 Diabetes
• Obesity
• Hypo- and Hyperthyroidism
• Acromegaly
• Chronic Renal Disease
• Cancer
Endocrine • Human• Mouse• Rat• Canine
Pituitaryo Human
o Rat
IGF-1o Human
o Rat/Mouse
Stresso Rat
Thyroido Rat
Available Panels:
HUMAN MOUSE CANINERAT
GIPGLP-2
GRP
PYYGastrinOxyntomodulin
VIP
CCK
Obestatin
Skin AdrenalCortex
LegendMSH = Melanocyte Stimulating Hormone
ACTH = Adrenocorticotropin Hormone
TSH = Thyroid-Stimulating Hormone
GH = Growth Hormone
PRL = Prolactin
OT = Oxytocin
FSH = Follicle Stimulating Hormone
LH = Luteinizing Hormone
Cortisol
MSH ACTH TSH GH
Growth Development and MetabolismGrowth Development and Metabolism
ReproductionReproduction
PRL FSHOT LH
Thyroid
T3, T4
Variousorgans
andtissues
Mammaryglands
developmentand milk
production
Uterussmoothmuscle
Gonad: ovary and testis
cleGerm cell
development
Female:Oestrogen andProgesterone
Male:Testosterone
Hormoneproduction
Pituitary Hormone Biological Functions
MILLIPLEX MAP Endocrine PanelsThe endocrine system is made up of small, ductless, and highly vascular organs
that secrete hormones, extracellular signaling molecules that regulate a variety
of functions, including growth and development, tissue function, metabolism
and even mood. Typical endocrine glands include the pituitary, thyroid and
adrenal glands. However, endocrine hormones secreted from the gut and
adipose tissue also play a critical role in regulating metabolism. Disruption of
hormone production, release or function, resulting in a hypo- or hyperfunction of
endocrine glands, is responsible for a variety of diseases: type 1 and 2 diabetes,
obesity, hypothyroidism, acromegaly and cancer.
AVAILABLE ANALYTES
Human
Mouse
Rat
Canine
*Amylin (active) and Amylin (total) cannot be plexed together.
Am
ylin
* (a
ctiv
e)
Am
ylin
* (t
otal
)
C-P
epti
de
GLP
-1 (ac
tive
)
Glu
cago
n
Insu
lin
Lept
in
Graphs demonstrate the correlation of several MILLIPLEX MAP endocrine system analytes with Millipore RIAs.
LH CorrelationMILLIPLEX MAP vs. RIA
25,000
20,000
15,000
10,000
5,000
00 5,000 10,000 15,000
MIL
LIPL
EX M
AP
– LH
(p
g/m
L)
RIA – LH (pg/mL)
y = 1.4957x – 1036.2R = 0.98
FSH Correlation (Rat Plasma Samples)MILLIPLEX MAP vs. ELISA
20
15
10
5
0
0 1 2 4
MIL
LIPL
EX M
AP
– FS
H
(pg/
mL)
RIA – FSH (pg/mL)
y = 4.8624x – 0.8678R = 0.96
y = 10.762x – 22.28R = 0.98
y = 2.4615x – 7211R = 0.89
3
ACTH CorrelationMILLIPLEX MAP vs. RIA
1,600
1,200
800
400
00 1,000 5,0004,0003,0002,000
MIL
LIPL
EX M
AP
– A
CTH
(p
g/m
L)
RIA – ACTH (pg/mL)
PRL CorrelationMILLIPLEX MAP vs. RIA
70,000
50,000
30,000
10,000
0 1,000 5,0004,0003,0002,000
MIL
LIPL
EX M
AP
– PR
L (p
g/m
L)
RIA – PRL (pg/mL)
6,000 7,000
FSH CorrelationMILLIPLEX MAP vs. RIA
140,000
100,000
60,000
20,000
0 10,000 50,00040,00030,00020,000
MIL
LIPL
EX M
AP
– FS
H
(pg/
mL)
RIA – FSH (pg/mL)
PRL CorrelationMILLIPLEX MAP vs. ELISA
70
60
50
40
30
20
10
0
0 10 20 60
MIL
LIPL
EX M
AP
– LH
(p
g/m
L)
ELISA – PRL (pg/mL)
30 40 50
FSH Correlation (Rat Serum Samples)MILLIPLEX MAP vs. ELISA
35
30
25
20
15
10
5
00 1 2
MIL
LIPL
EX M
AP
– FS
H
(pg/
mL)
ELISA – FSHL (pg/mL)
3 4
y = 1.1203x – 2.0113R = 0.95
y = 7.8427x – 3.2567R = 0.95
y = 0.3333x – 113.83R = 0.85
LH CorrelationMILLIPLEX MAP vs. RIA
25,000
20,000
15,000
10,000
5,000
00 5,000 10,000 15,000
MIL
LIPL
EX M
AP
– LH
(p
g/m
L)
RIA – LH (pg/mL)
y = 1.4957x – 1036.2R = 0.98
FSH Correlation (Rat Plasma Samples)MILLIPLEX MAP vs. ELISA
20
15
10
5
0
0 1 2 4
MIL
LIPL
EX M
AP
– FS
H
(pg/
mL)
RIA – FSH (pg/mL)
y = 4.8624x – 0.8678R = 0.96
y = 10.762x – 22.28R = 0.98
y = 2.4615x – 7211R = 0.89
3
ACTH CorrelationMILLIPLEX MAP vs. RIA
1,600
1,200
800
400
00 1,000 5,0004,0003,0002,000
MIL
LIPL
EX M
AP
– A
CTH
(p
g/m
L)
RIA – ACTH (pg/mL)
PRL CorrelationMILLIPLEX MAP vs. RIA
70,000
50,000
30,000
10,000
0 1,000 5,0004,0003,0002,000
MIL
LIPL
EX M
AP
– PR
L (p
g/m
L)
RIA – PRL (pg/mL)
6,000 7,000
FSH CorrelationMILLIPLEX MAP vs. RIA
140,000
100,000
60,000
20,000
0 10,000 50,00040,00030,00020,000
MIL
LIPL
EX M
AP
– FS
H
(pg/
mL)
RIA – FSH (pg/mL)
PRL CorrelationMILLIPLEX MAP vs. ELISA
70
60
50
40
30
20
10
0
0 10 20 60
MIL
LIPL
EX M
AP
– LH
(p
g/m
L)
ELISA – PRL (pg/mL)
30 40 50
FSH Correlation (Rat Serum Samples)MILLIPLEX MAP vs. ELISA
35
30
25
20
15
10
5
00 1 2
MIL
LIPL
EX M
AP
– FS
H
(pg/
mL)
ELISA – FSHL (pg/mL)
3 4
y = 1.1203x – 2.0113R = 0.95
y = 7.8427x – 3.2567R = 0.95
y = 0.3333x – 113.83R = 0.85
LH CorrelationMILLIPLEX MAP vs. RIA
25,000
20,000
15,000
10,000
5,000
00 5,000 10,000 15,000
MIL
LIPL
EX M
AP
– LH
(p
g/m
L)
RIA – LH (pg/mL)
y = 1.4957x – 1036.2R = 0.98
FSH Correlation (Rat Plasma Samples)MILLIPLEX MAP vs. ELISA
20
15
10
5
0
0 1 2 4
MIL
LIPL
EX M
AP
– FS
H
(pg/
mL)
RIA – FSH (pg/mL)
y = 4.8624x – 0.8678R = 0.96
y = 10.762x – 22.28R = 0.98
y = 2.4615x – 7211R = 0.89
3
ACTH CorrelationMILLIPLEX MAP vs. RIA
1,600
1,200
800
400
00 1,000 5,0004,0003,0002,000
MIL
LIPL
EX M
AP
– A
CTH
(p
g/m
L)
RIA – ACTH (pg/mL)
PRL CorrelationMILLIPLEX MAP vs. RIA
70,000
50,000
30,000
10,000
0 1,000 5,0004,0003,0002,000
MIL
LIPL
EX M
AP
– PR
L (p
g/m
L)
RIA – PRL (pg/mL)
6,000 7,000
FSH CorrelationMILLIPLEX MAP vs. RIA
140,000
100,000
60,000
20,000
0 10,000 50,00040,00030,00020,000
MIL
LIPL
EX M
AP
– FS
H
(pg/
mL)
RIA – FSH (pg/mL)
PRL CorrelationMILLIPLEX MAP vs. ELISA
70
60
50
40
30
20
10
0
0 10 20 60
MIL
LIPL
EX M
AP
– LH
(p
g/m
L)
ELISA – PRL (pg/mL)
30 40 50
FSH Correlation (Rat Serum Samples)MILLIPLEX MAP vs. ELISA
35
30
25
20
15
10
5
00 1 2
MIL
LIPL
EX M
AP
– FS
H
(pg/
mL)
ELISA – FSHL (pg/mL)
3 4
y = 1.1203x – 2.0113R = 0.95
y = 7.8427x – 3.2567R = 0.95
y = 0.3333x – 113.83R = 0.85
LH CorrelationMILLIPLEX MAP vs. RIA
25,000
20,000
15,000
10,000
5,000
00 5,000 10,000 15,000
MIL
LIPL
EX M
AP
– LH
(p
g/m
L)
RIA – LH (pg/mL)
y = 1.4957x – 1036.2R = 0.98
FSH Correlation (Rat Plasma Samples)MILLIPLEX MAP vs. ELISA
20
15
10
5
0
0 1 2 4
MIL
LIPL
EX M
AP
– FS
H
(pg/
mL)
RIA – FSH (pg/mL)
y = 4.8624x – 0.8678R = 0.96
y = 10.762x – 22.28R = 0.98
y = 2.4615x – 7211R = 0.89
3
ACTH CorrelationMILLIPLEX MAP vs. RIA
1,600
1,200
800
400
00 1,000 5,0004,0003,0002,000
MIL
LIPL
EX M
AP
– A
CTH
(p
g/m
L)
RIA – ACTH (pg/mL)
PRL CorrelationMILLIPLEX MAP vs. RIA
70,000
50,000
30,000
10,000
0 1,000 5,0004,0003,0002,000
MIL
LIPL
EX M
AP
– PR
L (p
g/m
L)
RIA – PRL (pg/mL)
6,000 7,000
FSH CorrelationMILLIPLEX MAP vs. RIA
140,000
100,000
60,000
20,000
0 10,000 50,00040,00030,00020,000
MIL
LIPL
EX M
AP
– FS
H
(pg/
mL)
RIA – FSH (pg/mL)
PRL CorrelationMILLIPLEX MAP vs. ELISA
70
60
50
40
30
20
10
0
0 10 20 60
MIL
LIPL
EX M
AP
– LH
(p
g/m
L)
ELISA – PRL (pg/mL)
30 40 50
FSH Correlation (Rat Serum Samples)MILLIPLEX MAP vs. ELISA
35
30
25
20
15
10
5
00 1 2
MIL
LIPL
EX M
AP
– FS
H
(pg/
mL)
ELISA – FSHL (pg/mL)
3 4
y = 1.1203x – 2.0113R = 0.95
y = 7.8427x – 3.2567R = 0.95
y = 0.3333x – 113.83R = 0.85
Human Endocrine Panel
Specificity
Cross-reactivity between the antibodies and any of the
other analytes in this panel is non-detectable or negligible.
Assay Conditions
• Amylin (active) and Amylin (total) cannot be plexed
together.
• This kit may be used for the analysis of either active
amylin or total amylin with any or all of the analytes in
the panel in serum, plasma, other body fluids, and
cell/tissue extract or culture samples.
• This is an overnight assay requiring 25 µL sample size.
PERFORMANCE SPECIFICATIONS
10,000
100
1 100 1,000
Concentration (pM)
MFI
10 10,000
100,000
1,000
Amylin
Leptin
Glucagon
aGLP-1
C-Peptide
Insulin
10,000
0
1 100 1,000
Concentration (pM)
MFI
10 10,000
100,000
1,000
100
aAmylin
Leptin
Glucagon
Insulin
aGLP-1
10,000
1
1 100 1,000
Concentration (pM)
MFI
10 10,000
100,000
1,000
100
10
aAmylin
Leptin
Glucagon
Insulin
aGLP-1
10,000
1
1 100 1,000
Concentration (pM)
MFI
10 10,000 100,000
100,000
1,000
100
10
Leptin
Glucagon
Insulin
GLP-1
MILLIPLEX MAP Human Endocrine 6-plex Assay
Standard Curves
MILLIPLEX MAP Mouse Endocrine Panel
Standard Curves in Serum Matrix
MILLIPLEX MAP Rat Endocrine Panel
Standard Curves
MILLIPLEX MAP Canine Endocrine Panel
Standard Curves
Hormone Molecular Weight pM ng/mL
Amylin 3903 100 0.39
C-Peptide 3617 100 0.36
GLP-1 (active) 3297 100 0.33
Glucagon 3483 100 0.34
Insulin 5808 100 0.58
Leptin 16027 100 1.6
HormoneStandard Curve
Ranges (pM)Sensitivity MinDC
(pM)
Precision
Accuracy (%)
Intra-Assay (%CV) Inter-Assay (%CV)
Amylin* (active)
6.2 – 4,500
1.5 3.8 8.4 80
Amylin* (total) 2.3 4.7 9.3 94
C-Peptide 2.7 7.3 4.2 84
GLP-1 (active) 3.4 5.1 7.4 136
Glucagon 2.2 4.9 7.1 93
Insulin 3.0 2.6 7.2 83
Leptin 8.7 5.1 7.4 103
*Amylin (active) and Amylin (total) cannot be plexed together.
HUMAN
STANDARD UNIT COMPARISON
Mouse Endocrine Panel
Specificity
Cross-reactivity between the antibodies and any of the
other analytes in this panel is non-detectable or negligible.
Assay Conditions
• This kit may be used for the analysis of all or any
combination of the analytes in this panel in serum,
plasma, other body fluids, and cell/tissue extract or
culture sample.
• This is an overnight assay requiring 10 µL sample size.
PERFORMANCE SPECIFICATIONS
10,000
100
1 100 1,000
Concentration (pM)
MFI
10 10,000
100,000
1,000
Amylin
Leptin
Glucagon
aGLP-1
C-Peptide
Insulin
10,000
0
1 100 1,000
Concentration (pM)
MFI
10 10,000
100,000
1,000
100
aAmylin
Leptin
Glucagon
Insulin
aGLP-1
10,000
1
1 100 1,000
Concentration (pM)
MFI
10 10,000
100,000
1,000
100
10
aAmylin
Leptin
Glucagon
Insulin
aGLP-1
10,000
1
1 100 1,000
Concentration (pM)
MFI
10 10,000 100,000
100,000
1,000
100
10
Leptin
Glucagon
Insulin
GLP-1
MILLIPLEX MAP Human Endocrine 6-plex Assay
Standard Curves
MILLIPLEX MAP Mouse Endocrine Panel
Standard Curves in Serum Matrix
MILLIPLEX MAP Rat Endocrine Panel
Standard Curves
MILLIPLEX MAP Canine Endocrine Panel
Standard Curves
Hormone Molecular Weight pM ng/mL
Amylin 3921 100 0.58
GLP-1 (active) 3297 100 0.33
Glucagon 3483 100 0.35
Insulin 5800 100 0.58
Leptin 16162 100 1.6
AnalyteStandard
Curve Range (pg/mL)
Sensitivity (pM)
Precision Accuracy (%)
Intra-Assay (%CV)
Inter-Assay (%CV)
Serum Matrix (%)
Serum Samples (%)
Amylin
6.2 to 4,500
6.2
3.8 – 10.6 4.8 – 20.7
101 80
GLP-1 (active) 6.2 101 115
Glucagon 6.2 99.7 106
Insulin 55.6 100 106
Leptin 6.2 101 97
MOUSE
STANDARD UNIT COMPARISON
Hormone Molecular Weight pM ng/mL
Amylin 3921 100 0.58
GLP-1 (active) 3297 100 0.33
Glucagon 3483 100 0.35
Insulin 5800 100 0.58
Leptin 16162 100 1.6
Rat Endocrine Panel
Specificity
Cross-reactivity between the antibodies and any of the
other analytes in this panel is non-detectable or negligible.
Assay Conditions
• This kit may be used for the analysis of all or any
combination of the analytes in this panel in serum,
plasma, other body fluids, and cell/tissue extract or
culture samples.
• This is an overnight assay requiring 10 µL sample size.
PERFORMANCE SPECIFICATIONS
10,000
100
1 100 1,000
Concentration (pM)
MFI
10 10,000
100,000
1,000
Amylin
Leptin
Glucagon
aGLP-1
C-Peptide
Insulin
10,000
0
1 100 1,000
Concentration (pM)
MFI
10 10,000
100,000
1,000
100
aAmylin
Leptin
Glucagon
Insulin
aGLP-1
10,000
1
1 100 1,000
Concentration (pM)
MFI
10 10,000
100,000
1,000
100
10
aAmylin
Leptin
Glucagon
Insulin
aGLP-1
10,000
1
1 100 1,000
Concentration (pM)
MFI
10 10,000 100,000
100,000
1,000
100
10
Leptin
Glucagon
Insulin
GLP-1
MILLIPLEX MAP Human Endocrine 6-plex Assay
Standard Curves
MILLIPLEX MAP Mouse Endocrine Panel
Standard Curves in Serum Matrix
MILLIPLEX MAP Rat Endocrine Panel
Standard Curves
MILLIPLEX MAP Canine Endocrine Panel
Standard Curves
HormoneStandard Curve
Ranges (pM)Sensitivity MinDC
(pM)
PrecisionAccuracy
Serum Matrix (%)Intra-Assay (%CV) Inter-Assay (%CV)
Amylin (active) 17 – 18,000 6.2
3.8 – 10.6 4.8 – 20.7
101
GLP-1 (active) 23 – 23,000 6.2 101
Glucagon 15 – 6,000 6.2 99.7
Insulin 7 – 10,000 55.6 100
Leptin 98 – 7,000 6.2 101
RAT
STANDARD UNIT COMPARISON
HormoneStandard Curve Range (pg/mL)
Sensitivity MinDC (pg/mL)
Precision
Accuracy (%)
Intra-Assay (%CV) Inter-Assay (%CV)
GLP-1 (active) 20.6 to 15,000 12.1 5.6 14.6 73.7
Glucagon 5.5 to 4,000 5.1 10.2 17.8 96.4
Insulin 137.2 to 100,000 73.0 4.7 13.4 81.4
Leptin 137.2 to 100,000 148.0 4.6 17.6 55.1
Canine Endocrine Panel
Specificity
Cross-reactivity between the antibodies and any of the
other analytes in this panel is non-detectable or negligible.
Assay Conditions
• This kit may be used for the analysis of all or any
combination of the analytes in this panel in serum,
plasma, other body fluids, and cell/tissue extract or
culture samples.
• The assay requires a 1:3 dilution in serum or plasma
using assay buffer.
• Users will need to determine the appropriate dilution
when using cell or tissue culture supernatants.
• This is an overnight assay requiring 25 µL sample size.
PERFORMANCE SPECIFICATIONS
10,000
100
1 100 1,000
Concentration (pM)
MFI
10 10,000
100,000
1,000
Amylin
Leptin
Glucagon
aGLP-1
C-Peptide
Insulin
10,000
0
1 100 1,000
Concentration (pM)
MFI
10 10,000
100,000
1,000
100
aAmylin
Leptin
Glucagon
Insulin
aGLP-1
10,000
1
1 100 1,000
Concentration (pM)
MFI
10 10,000
100,000
1,000
100
10
aAmylin
Leptin
Glucagon
Insulin
aGLP-1
10,000
1
1 100 1,000
Concentration (pM)
MFI
10 10,000 100,000
100,000
1,000
100
10
Leptin
Glucagon
Insulin
GLP-1
MILLIPLEX MAP Human Endocrine 6-plex Assay
Standard Curves
MILLIPLEX MAP Mouse Endocrine Panel
Standard Curves in Serum Matrix
MILLIPLEX MAP Rat Endocrine Panel
Standard Curves
MILLIPLEX MAP Canine Endocrine Panel
Standard Curves
CANINE
MILLIPLEX MAP Human and Rat/Mouse IGF-1 Single PlexesInsulin-like Growth Factor (IGF-1) is a polypeptide endocrine
hormone similar in structure to insulin. Stimulated by growth
hormone and produced primarily in the liver, it binds to the
IGF-1 receptor with a high affinity and to the insulin receptor
with a much lower affinity. The tyrosine kinase IGF-1 receptor
is a potent activator of the Akt signaling pathway, which drives
a variety of processes including cell growth and division and
apoptosis inhibition. Because approximately 98% of IGF-1 is
always bound to one of six binding proteins (IGF-BP), extraction
may be required to measure extracellular protein concentrations.
Increased levels of IGF-1 have been correlated with an increased
risk of cancer. In addition, while involved in reducing hemoglobin
A1C levels and daily insulin consumption in types 1 and 2
diabetes, these increased levels have also been implicated in the
development of diabetic retinopathy. Drugs have been developed
as replacement therapy for severe primary IGF-1 deficiency, a
group of diseases in which patients do not respond to growth
hormone therapy.
1,000
10
100 1,000
Concentration (pg/mL)
MFI
10,000 100,000
10,000
100
10,000
101 1,000
Concentration (pg/mL)
MFI
10 100 10,000 100,000
100,000
1,000
100
MILLIPLEX MAP Human IGF-1 Single Plex
Standard Curve
MILLIPLEX MAP Rat/Mouse IGF-1 Single Plex
Standard Curve
1,000
10
100 1,000
Concentration (pg/mL)
MFI
10,000 100,000
10,000
100
10,000
101 1,000
Concentration (pg/mL)M
FI
10 100 10,000 100,000
100,000
1,000
100
MILLIPLEX MAP Human IGF-1 Single Plex
Standard Curve
MILLIPLEX MAP Rat/Mouse IGF-1 Single Plex
Standard Curve
Standard Curve Range
Human: 137 to 100,000 pg/mL
Rat/Mouse: 3.2 to 10,000 pg/mL
Sensitivity
Human: 60 pg/mL
Rat/Mouse: 3.2 pg/mL
Accuracy
Human: 84%
Rat/Mouse: 99%
Precision
Human
Intra-assay: < 5% Inter-assay: < 12%
Rat/Mouse
Intra-assay: < 10% Inter-assay: < 5%
Specificity
The Ab pair used in each assay is specific and displays
no cross-reactivity to insulin or IGF-2.
Assay Conditions
Human
• This assay may be used for the analysis human IGF-1 in
serum, plasma, other body fluids, and cell/tissue extract
or culture samples
• Sample extraction is required for the measurement of
IGF-1 in serum or plasma.
• This is an overnight assay requiring 25 µL sample size.
Rat/Mouse
• This assay may be used for the analysis of rat/mouse
IGF-1 in serum, plasma, other body fluids, and cell/tissue
extract or culture samples.
• This single plex requires a 1:200 dilution of 25 µL of
serum or plasma in assay buffer. Users need to
determine appropriate dilutions in cell/tissue extracts or
culture samples.
• This is an overnight assay requiring 25 µL sample size.
PERFORMANCE SPECIFICATIONS
AVAILABLE ANALYTES
Human
Rat
Adr
enoc
orti
cotr
opic
H
orm
one
(ACTH
)
Bra
in D
eriv
ed N
euro
trop
hic
Fact
or (B
DN
F)
Folli
cle-
Sti
mul
atin
g H
orm
one
(FSH
)
Gro
wth
Hor
mon
e(G
H)
Lute
iniz
ing
Hor
mon
e(L
H)
Prol
acti
n (P
RL)
Thy
roid
-Sti
mul
atin
g H
orm
one
(TSH
)
MILLIPLEX MAP Pituitary Multiplex PanelsCalled the “master gland” because it controls many other endocrine glands, the
pituitary gland secretes a number of key hormones that play important roles
in the regulation of metabolism, growth, and reproduction. For example, these
hormones are responsible for the stimulation of: the adrenal gland (ACTH),
thyroid gland (TSH), ovaries and testes (FSH, LH) and breast milk production
(prolactin), as well as blood pressure and the amount of water excreted by
the kidneys. In turn, the pituitary gland is connected to and controlled by
the hypothalamus, which determines the amount of stimulation needed by the
pituitary’s target glands by detection of their hormone levels.
Graphs demonstrate the correlation of several MILLIPLEX MAP endocrine system analytes with Millipore RIAs and ELISAs.
See page 3 for the remaining graphs.
LH CorrelationMILLIPLEX MAP vs. RIA
25,000
20,000
15,000
10,000
5,000
00 5,000 10,000 15,000
MIL
LIPL
EX M
AP
– LH
(p
g/m
L)
RIA – LH (pg/mL)
y = 1.4957x – 1036.2R = 0.98
FSH Correlation (Rat Plasma Samples)MILLIPLEX MAP vs. ELISA
20
15
10
5
0
0 1 2 4
MIL
LIPL
EX M
AP
– FS
H
(pg/
mL)
RIA – FSH (pg/mL)
y = 4.8624x – 0.8678R = 0.96
y = 10.762x – 22.28R = 0.98
y = 2.4615x – 7211R = 0.89
3
ACTH CorrelationMILLIPLEX MAP vs. RIA
1,600
1,200
800
400
00 1,000 5,0004,0003,0002,000
MIL
LIPL
EX M
AP
– A
CTH
(p
g/m
L)
RIA – ACTH (pg/mL)
PRL CorrelationMILLIPLEX MAP vs. RIA
70,000
50,000
30,000
10,000
0 1,000 5,0004,0003,0002,000
MIL
LIPL
EX M
AP
– PR
L (p
g/m
L)
RIA – PRL (pg/mL)
6,000 7,000
FSH CorrelationMILLIPLEX MAP vs. RIA
140,000
100,000
60,000
20,000
0 10,000 50,00040,00030,00020,000
MIL
LIPL
EX M
AP
– FS
H
(pg/
mL)
RIA – FSH (pg/mL)
PRL CorrelationMILLIPLEX MAP vs. ELISA
70
60
50
40
30
20
10
0
0 10 20 60
MIL
LIPL
EX M
AP
– LH
(p
g/m
L)
ELISA – PRL (pg/mL)
30 40 50
FSH Correlation (Rat Serum Samples)MILLIPLEX MAP vs. ELISA
35
30
25
20
15
10
5
00 1 2
MIL
LIPL
EX M
AP
– FS
H
(pg/
mL)
ELISA – FSHL (pg/mL)
3 4
y = 1.1203x – 2.0113R = 0.95
y = 7.8427x – 3.2567R = 0.95
y = 0.3333x – 113.83R = 0.85
LH CorrelationMILLIPLEX MAP vs. RIA
25,000
20,000
15,000
10,000
5,000
00 5,000 10,000 15,000
MIL
LIPL
EX M
AP
– LH
(p
g/m
L)
RIA – LH (pg/mL)
y = 1.4957x – 1036.2R = 0.98
FSH Correlation (Rat Plasma Samples)MILLIPLEX MAP vs. ELISA
20
15
10
5
0
0 1 2 4
MIL
LIPL
EX M
AP
– FS
H
(pg/
mL)
RIA – FSH (pg/mL)
y = 4.8624x – 0.8678R = 0.96
y = 10.762x – 22.28R = 0.98
y = 2.4615x – 7211R = 0.89
3
ACTH CorrelationMILLIPLEX MAP vs. RIA
1,600
1,200
800
400
00 1,000 5,0004,0003,0002,000
MIL
LIPL
EX M
AP
– A
CTH
(p
g/m
L)
RIA – ACTH (pg/mL)
PRL CorrelationMILLIPLEX MAP vs. RIA
70,000
50,000
30,000
10,000
0 1,000 5,0004,0003,0002,000
MIL
LIPL
EX M
AP
– PR
L (p
g/m
L)
RIA – PRL (pg/mL)
6,000 7,000
FSH CorrelationMILLIPLEX MAP vs. RIA
140,000
100,000
60,000
20,000
0 10,000 50,00040,00030,00020,000
MIL
LIPL
EX M
AP
– FS
H
(pg/
mL)
RIA – FSH (pg/mL)
PRL CorrelationMILLIPLEX MAP vs. ELISA
70
60
50
40
30
20
10
0
0 10 20 60
MIL
LIPL
EX M
AP
– LH
(p
g/m
L)
ELISA – PRL (pg/mL)
30 40 50
FSH Correlation (Rat Serum Samples)MILLIPLEX MAP vs. ELISA
35
30
25
20
15
10
5
00 1 2
MIL
LIPL
EX M
AP
– FS
H
(pg/
mL)
ELISA – FSHL (pg/mL)
3 4
y = 1.1203x – 2.0113R = 0.95
y = 7.8427x – 3.2567R = 0.95
y = 0.3333x – 113.83R = 0.85
LH CorrelationMILLIPLEX MAP vs. RIA
25,000
20,000
15,000
10,000
5,000
00 5,000 10,000 15,000
MIL
LIPL
EX M
AP
– LH
(p
g/m
L)
RIA – LH (pg/mL)
y = 1.4957x – 1036.2R = 0.98
FSH Correlation (Rat Plasma Samples)MILLIPLEX MAP vs. ELISA
20
15
10
5
0
0 1 2 4
MIL
LIPL
EX M
AP
– FS
H
(pg/
mL)
RIA – FSH (pg/mL)
y = 4.8624x – 0.8678R = 0.96
y = 10.762x – 22.28R = 0.98
y = 2.4615x – 7211R = 0.89
3
ACTH CorrelationMILLIPLEX MAP vs. RIA
1,600
1,200
800
400
00 1,000 5,0004,0003,0002,000
MIL
LIPL
EX M
AP
– A
CTH
(p
g/m
L)
RIA – ACTH (pg/mL)
PRL CorrelationMILLIPLEX MAP vs. RIA
70,000
50,000
30,000
10,000
0 1,000 5,0004,0003,0002,000
MIL
LIPL
EX M
AP
– PR
L (p
g/m
L)
RIA – PRL (pg/mL)
6,000 7,000
FSH CorrelationMILLIPLEX MAP vs. RIA
140,000
100,000
60,000
20,000
0 10,000 50,00040,00030,00020,000
MIL
LIPL
EX M
AP
– FS
H
(pg/
mL)
RIA – FSH (pg/mL)
PRL CorrelationMILLIPLEX MAP vs. ELISA
70
60
50
40
30
20
10
0
0 10 20 60
MIL
LIPL
EX M
AP
– LH
(p
g/m
L)
ELISA – PRL (pg/mL)
30 40 50
FSH Correlation (Rat Serum Samples)MILLIPLEX MAP vs. ELISA
35
30
25
20
15
10
5
00 1 2
MIL
LIPL
EX M
AP
– FS
H
(pg/
mL)
ELISA – FSHL (pg/mL)
3 4
y = 1.1203x – 2.0113R = 0.95
y = 7.8427x – 3.2567R = 0.95
y = 0.3333x – 113.83R = 0.85
Human Pituitary Panel
Rat Pituitary Panel
Specificity
Cross-reactivity between the antibodies and any of the
other analytes in this panel is non-detectable or negligible.
Assay Conditions
• This kit may be used for the analysis of all or any
combination of the analytes in this panel in serum,
plasma, other body fluids, and cell/tissue extract or
culture samples.
• This is a 4-hour assay requiring 25 µL sample size.
Specificity
Cross-reactivity between the antibodies and any of the
other analytes in this panel is non-detectable or negligible.
Assay Conditions
• This kit may be used for the analysis of all or any
combination of the analytes in this panel in serum,
plasma, other body fluids, and cell culture samples.
• This is an overnight assay requiring 10 µL sample size.
PERFORMANCE SPECIFICATIONS
PERFORMANCE SPECIFICATIONS
10,000
10
.001 0.1 1
Concentration (ng/mL, µIU/mL, or mIU/mL)
MFI
0.01 10 100
100,000
1,000
100
FSH
LH
TSH
PRL
GH
ACTH
10,000
10
1 100 1,000
Concentration (pg/mL)
MFI
10 10,000 100,000
100,000
1,000
100
FSH
LH
TSH
PRL
GH
ACTH
BDNF
MILLIPLEX MAP Human Pituitary Panel
Standard Curves
MILLIPLEX MAP Rat Pituitary Hormone Panel
Standard Curves
10,000
10
.001 0.1 1
Concentration (ng/mL, µIU/mL, or mIU/mL)
MFI
0.01 10 100
100,000
1,000
100
FSH
LH
TSH
PRL
GH
ACTH
10,000
10
1 100 1,000
Concentration (pg/mL)
MFI
10 10,000 100,000
100,000
1,000
100
FSH
LH
TSH
PRL
GH
ACTH
BDNF
MILLIPLEX MAP Human Pituitary Panel
Standard Curves
MILLIPLEX MAP Rat Pituitary Hormone Panel
Standard Curves
HormoneStandard Curve Range (pg/mL)
Sensitivity MinDC (pg/mL)
Precision
Accuracy (%)
Intra-Assay (%CV) Inter-Assay (%CV)
ACTH 0.003 to 12.5 ng/mL 0.003 ng/mL 10.8 7.4 90.6
FSH 0.024 to 100 mIU/mL 0.005 mIU/mL 7.2 4.9 92.3
GH 0.012 to 50 ng/mL 0.004 ng/mL 5.4 14.9 76.4
LH 0.049 to 200 mIU/mL 0.007 mIU/mL 6.3 6.7 89.5
Prolactin 0.024 to 100 ng/mL 0.004 ng/mL 7.0 8.2 91.6
TSH 0.012 to 50 µIU/mL 0.014 µIU/mL 6.9 3.0 113.2
HormoneStandard Curve Range (pg/mL)
Sensitivity MinDC (pg/mL)
Precision
Accuracy (%)
Intra-Assay (%CV) Inter-Assay (%CV)
ACTH 3.2 –10,000 2.1
< 9.5 < 15
99
BDNF 3.2 –10,000 0.4 114
FSH 3.2 –10,000 47.7 99
GH 16 – 50,000 12.0 95
LH 3.2 –10,000 4.9 85
PRL 16 – 50,000 9.0 96
TSH 3.2 –10,000 6.4 93
HUMAN RAT
MILLIPLEX MAP Stress Hormone Multiplex PanelsStress is defined as the disruption of homeostasis through physical and/or
psychological stimuli (stressors). Physiologically the neuroendocrine system,
which includes the hypothalamus, pituitary and adrenal glands, plays a critical
role in response to stress. Activated by corticotropin-releasing hormone (CRH)
and arginine-vasopressin (AV), the pituitary gland releases ACTH which results
in the secretion of cortisol / corticosterone and other glucocorticoids from the
adrenal glands. These corticoids involve the whole body in the stress response
and are finally involved in the termination of the stress response by a negative
feedback loop to the hypothalamus.
Rat Stress Hormone Panel
Specificity
Cross-reactivity between the antibodies and any of the
other analytes in this panel is non-detectable or negligible.
Assay Conditions
• This kit may be used for the analysis of all or any
combination of the analytes in this panel in serum,
plasma, other body fluids, and cell/tissue extract or
culture samples.
• This single plex requires a 1:3 dilution of 25 µL of serum
or plasma in assay buffer. Users need to determine
appropriate delusions in cell/tissue extracts or culture
samples.
• This is an overnight assay requiring 25 µL sample size.
PERFORMANCE SPECIFICATIONS
12,000
0
10 1,000 10,000
Concentration (pg/mL)
MFI
100 100,000 1,000,000
16,000
8,000
4,000
ACTH
Melatonin
Corticosterone
100
10 1,000 10,000
Concentration (pg/mL)
MFI
100 100,000 1,000,000
10,000
1,000
T3
T4
TSH
MILLIPLEX MAP Rat Stress Hormone Panel
Standard Curves
MILLIPLEX MAPRat Thyroid Hormone Panel
Standard Curves
ACTH: Sandwich AssayMelatonin & Corticosterone: Competitive Assays
HormoneStandard Curve Range (pg/mL)
Sensitivity (pg/mL)
Precision
Accuracy (%)
Intra-Assay (%CV) Inter-Assay (%CV)
ACTH 16.5 –4,000 3.8
< 7 < 15
111
Corticosterone 1,646 – 400,000 10,834 94
Melatonin 1,646 – 400,000 897 155
AVAILABLE ANALYTES
Rat
Adrenocorticotropic Hormone (ACTH) Corticosterone Melatonin
RAT
MILLIPLEX MAP Thyroid Multiplex and Single Plex PanelsTriiodothyronine (T3) and thyroxine (T4) are key hormones secreted from the
thyroid gland with a wide-range of biological effects, including the metabolism
of carbohydrates, fatty acids, and proteins. In addition, these hormones play a
vital role in the development of the central nervous and skeletal systems. The
syntheses and release of T3 and T4 are regulated by Thyroid-Stimulating Hormone
(TSH), which is secreted from the anterior pituitary gland. Together, these thyroid
hormones play critical roles in the regulation of energy balance and mental and
physical development. According to some studies, the simultaneous measurement
of T3, T4 and TSH is of greater value than individual measurements, especially
when monitoring disease relapse or therapeutic response.
Rat Thyroid Panels
Specificity
Cross-reactivity between the antibodies and any of the
other analytes in this panel is non-detectable or negligible.
The Rat T3/T4 Two-Plex can be used to measure T3
and T4 in serum or plasma of mouse and other species.
Assay Conditions
• This kit may be used for the analysis of all or any
combination of the analytes in this panel in serum,
plasma and cell/tissue extract or culture samples.
• This is an overnight assay requiring 25 µL sample size.
PERFORMANCE SPECIFICATIONS
12,000
0
10 1,000 10,000
Concentration (pg/mL)M
FI100 100,000 1,000,000
16,000
8,000
4,000
ACTH
Melatonin
Corticosterone
100
10 1,000 10,000
Concentration (pg/mL)
MFI
100 100,000 1,000,000
10,000
1,000
T3
T4
TSH
MILLIPLEX MAP Rat Stress Hormone Panel
Standard Curves
MILLIPLEX MAPRat Thyroid Hormone Panel
Standard Curves
ACTH: Sandwich AssayMelatonin & Corticosterone: Competitive Assays
HormoneStandard Curve Range (pg/mL)
Sensitivity (pg/mL)
Precision
Accuracy (%)
Intra-Assay (%CV) Inter-Assay (%CV)
T3 41 – 10,000 99
< 5 < 10
111
T4 823 – 200,000 1,074 128
TSH 41 – 10,000 7.4 79
AVAILABLE ANALYTES
Rat Three-Plex
Rat Two-Plex
Rat Single Plex
Thyroid-Stimulating Hormone (TSH) Triiodothyronine (T3) Thyroxine (T4)
RAT
xMAP TECHNOLOGy PROCESS FLOW
With xMAP technology, you will benefit from
the ability to simultaneously quantitate up to
100 analytes in a single sample, using as little
as 25 µL or less.
• Speed/High-Throughput: Simultaneously measure
the concentration of a large number of analytes,
allowing you to do more faster, gaining early and
comprehensive data so critical to your work.
• Accuracy: xMAP technology generates real-time
analysis and accurate quantification of biological
interactions.
• Reproducibility: The high-volume production of
xMAP beads allows assay standardization that
solid-phased flat arrays cannot provide.
COMPARISON OF THE xMAP & ELISA TECHNOLOGIESSide-by-side comparison of Multiplexing vs. ELISAs (Study of 10 different analytes in 80 test samples).
xMAP Technology ELISA
Number of plates required 1 10
Results per plate 800 80
Total sample used per panel 50 µL 4 mL
Internal controls possible Yes No
Dynamic Range 1-10,000 pg/mL 10-2500 pg/mL
Lower limit of detection ~1 pg/mL ~1 pg/mL
Luminex xMAP Technology
1 Luminex uses proprietary techniques to internally color-code microspheres with two fluorescent dyes. Through precise concentrations of these dyes, 100 distinctly colored bead sets can be created, each of which is coated with a specific capture antibody.
3 The microspheres are allowed to pass rapidly through a laser, which excites the internal dyes marking the microsphere set. A second laser excites PE, the fluorescent dye on the reporter molecule.
2 After an analyte from a test sample is captured by the bead, a biotinylated detection antibody is introduced. The reaction mixture is then incubated with Streptavidin PE conjugate, the reporter molecule, to complete the reaction on the surface of each microsphere.
Bead #12, IL-6Bead #29, IFN-γ
Bead #56, TNF-α
COMPARISON OF THE xMAP & ELISA TECHNOLOGIESSide-by-side comparison of Multiplexing vs. ELISAs (Study of 10 different analytes in 80 test samples).
xMAP Technology ELISA
Number of plates required 1 10
Results per plate 800 80
Total sample used per panel 50 µL 4 mL
Internal controls possible Yes No
Dynamic Range 1-10,000 pg/mL 10-2500 pg/mL
Lower limit of detection ~1 pg/mL ~1 pg/mL
PRODUCTS • Higher Quality – Industry-leading QC, QA and Six Sigma Principles
• More Efficient – Analytically validated panels for consistency and reproducibility
• All-In-One Kit – A single kit with a single catalog number
• More Flexible – Select only the analytes needed or choose a pre-mixed kit
• Continuity of Knowledge Is the Key – Millipore “owns” every step of the process,
from R&D to manufacturing
• More Choices – The broadest range of multiplex analytes for the Luminex xMAP platform
INSTRUMENTS • Speed/High-Throughput – Simultaneously measure the concentration of a large number
of different analytes, allowing you to do more faster, gaining early and comprehensive
data so critical to your work.
• Accuracy – xMAP technology generates real-time analysis and accurate quantification of
the biological interactions.
• Reproducibility – High-volume production of xMAP microspheres allows assay
standardization that solid-phased flat arrays cannot provide.
• Low Sample Volume – With minimal hands-on time, you can screen more than 40
analytes in a single sample using as little as 25 µL or less.
SOFTWARE • The Luminex xPONENT® software helps you manage and track all of your multiplex
assays by combining ease of use and flexibility to perform both protein and nucleic acid
testing on one system. It’s also compatible with the upcoming magnetic bead (MagPlex™
beads) assays, and will be moving forward with compatible future instrument platforms.
• NEW! Millipore now offers MILLIPLEX Analyst software, developed exclusively for
Millipore by VigeneTech. This data analysis package helps you do your best work with
increased speed and efficiency. MILLIPLEX Analyst software provides a whole new level
of data analysis by allowing you to import large data sets and benefit from the ability to
calculate your results in seconds. The user-friendly plate setup feature lets you work
with speed and efficiency while the unique algorithms on even the most challenging data
sets give you the performance you need for multiplexing analysis.
SERVICES • Millipore’s BioMarker Services (BMS) has been performing immunoassays
for over 25 years.
• Assay services are available for RIAs, ELISAs, IRMAs and multiplex assays, using both
Millipore kits as well as any commercially available assays.
• Quality control, reproducible results, and confidentiality are of paramount importance
to our BMS team.
• Millipore’s Regulatory Compliant Laboratory is uniquely positioned to provide
bioanalytical services to support your preclinical and clinical studies.
MILLIPLEX MAP: Everything You Need – All In One Place
MILLIPLEX MAP
MILLIPLEX Analyzer
MILLIPLEX Analyst
BioPharma Services
For a complete and up-to-date list of MILLIPLEX MAP analytes, visit www.millipore.com/analytes.
TO PLACE AN ORDER Visit www.millipore.com/milliplex or call 1-800-MILLIPORE.
FOR PRODUCT OR APPLICATION INFORMATION Contact Technical Service at US Toll Free 1-866-441-8400,
1-636-441-8400, or www.millipore.com/techservice.
TO REQUEST A SALES REP Email: [email protected].
Millipore is a registered trademark of Millipore Corporation. The M mark, Advancing Life Science Together, and MILLIPLEX are trademarks of Millipore Corporation.Luminex, xPONENT, and xMAP are registered trademarks of Luminex Corporation. MagPlex is a trademark of Luminex Corporation. Lit. No. PB2275EN00 08DD013 Printed in U.S.A. 12/08 DD-SBU-08-01132© 2008 Millipore Corporation, Billerica, MA 01821 U.S.A. All rights reserved.
Description Quantity Catalogue No.
Endocrine Panels Human Endocrine Multiplex Panel 96-well plate HENDO-65K
Mouse Endocrine Multiplex Panel 96-well plate MENDO-75K
Rat Endocrine Multiplex Panel 96-well plate RENDO-85K
Canine Endocrine Multiplex Panel 96-well plate CENDO-95K
Human IGF-1 Single Plex 96-well plate HIGF1-52K-01
Rat/Mouse IGF-1 Single Plex 96-well plate RMIGF187K
Pituitary Panels Human Pituitary Multiplex Panel 96-well plate HPT-66K
Rat Pituitary Multiplex Panel 96-well plate RPT-86K
Rat Stress Hormone Multiplex Panel 96-well plate RSH69K
Thyroid Panels Rat Thyroid Hormone Three Plex 96-well plate RTHY-30K-03
Rat T3/T4 Two Plex 96-well plate RTHY-30K-T3T4
Rat TSH Single Plex 96-well plate RTHY-30K-TSH
ORDERING INFORMATION
www.millipore.com/offices
VigeneTech
Other Related Productso A variety of RIAs and ELISAs for the study of Metabolic Syndrome in many species:
human, mouse, rat, canine, porcine, bovine
o MILLIPLEX MAP Multiplex Panels:
- Adipokine / Adipocyte: human, mouse, rat, canine
- Adiponectin Single Plex: mouse, rat
- Gut Hormone: human, mouse, rat
- Cytokine/Chemokine: human, high sensitivity human,
non-human primate, mouse, rat, canine