Microarrays – A New Revolution: Genomic Expression Analysis of

Oxidative Stress Genes in Saccharomyces cerevisiae

Dana AricoDana AricoBetsy KarleBetsy Karle

Jeff Kucharski Jeff Kucharski Kumo Lartevi Kumo LarteviDr. Terrie RifeDr. Terrie Rife

Microarray Background

•Allows researchers to analyze the expression of thousands of genes at one time.

•This technique can be used to identify and analyze mutations in DNA

•Model organisms, whose genome has been completely sequenced, are used in microarray experiment.

First Microarray Experiment

• Was performed by researchers from the NIH, Stanford University, and others

• Wanted to compare gene expression of patients with B-cell Lymphoma

• Patients fell under two categories: • Long-term survivors

• Short-term survivors

Preparing the DNA Chip

cDNA

Arrayed

Genes

Robotic

Printing

Cloning &

Sequencing

Preparing the Samples

Long-term Survivors / Short-term Survivors

Extraction

RNA

cDNA &

Labeling

RT reaction

The Whole Picture

Arrayed

Genes

Labeled

cDNA

Hybridized

Array

Hybridization

Gene Expression

Long-term survivors Short-term survivors

1) I 1)

2) II 2) II

3) 3) I

I

II II

II

I

I

Thousands of Genes