magnification and microscopes
DESCRIPTION
microscpesTRANSCRIPT
Regents Biology
Microscopes!
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Mutual dependence: Science & Technology
Technology…the pragmatist Does it work? Is it useful? Is it sustainable?
Science…the idealist What is it? How does it work? What does it tell us?
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Mutual dependence: Science & Technology
Microscopes Electron microscopes
Higher resolution Dead samples Expensive
Optical (light) microscopes Lower resolution Live samples Less expensive
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Micrographs
Scanning electron micrograph (SEM) of various Pollen. Public domain image reference: Dartmouth Electron Microscope Facility, Dartmouth College
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Size matters micron = micrometer = 1/1,000,000 meter diameter of human hair = ~20 microns
Miss N, Show them the interactive!!
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Microscopic units of measurement
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1 metre (m) = ________ millimetres (mm)
1 mm = ________ microns (µm)
1 µm = ________ nanometres (nm)
1 mm = ________ nm
1 m = ________ µm
1 m =________ nm
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1 m = 1,000 mm = 103mm
1 mm = 1,000 µm= 103µm
1 µm = 1,000 nm= 103nm
1 mm = 1,000,000 nm = 106nm
1 m = 1,000,000 µm = 106µm
1 m = 1,000,000,000nm =109 nm
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10-3 m = ______ m = _____mm
10-6 m = ______ m = _____mm
10-9 m = ______ m = _____mm
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10-3 m = 0.001m = 1 mm
10-6 m = 0.000001 = 1 µm
10-9 m = 0.000000001= 1nm
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Electron microscope range:
_____ to _____
Light microscope range:
_____ to _____
Unaided human eye range:
_____ to _____Unaided?
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Electron microscope range:
1 nm to 100 µm
Light microscope range:
1 µm to 1 mm
Unaided human eye range:
1 mm upwards
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Name that micrograph
Light microscope or Electron microscope?
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Regents Biology19 Light microscope
Unstained
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Regents Biology20 Light microscope
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Regents Biology22 Light microscope
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Tick
Regents Biology23 Electron microscope
Tick
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Regents Biology24 Electron microscope
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Regents Biology25 Light microscope
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Regents Biology26 Electron microscope
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Aphid
Regents Biology27 Electron microscope
Aphid
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Regents Biology28 Light microscope
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Regents Biology29 Light microscope
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Regents Biology30 Electron microscope
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Regents Biology31 Electron microscope
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Regents Biology32 Electron microscope
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Regents Biology33 Electron microscope
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Regents Biology35 Light microscope
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Regents Biology36 Light microscope
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Regents Biology37 Light microscope
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THE END!
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Magnification
Magnification = ocular lens x objective lens
A microscope has an eyepiece lens with a power of 20X. The objective lens being used has a power of 10X. What is the total magnification?
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Using Scale Bars
Using a scale bar:The image at right is of a virus-like particle. The bar represents 50nm. It is observed under x200. Calculate the actual size of the image.
50nm
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Image is 50nm in diameter
Using a scale bar:
50nm
Using Scale Bars
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A specimen is 1cm in diameter under a power of x600. What is the actual size of the specimen?
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Magnification: I AM
Watch yo units!!
Image size
Actual size Magnification
Measure with ruler
Need to figure this out
Given to us
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1. Measure the size of the image. …..1cm (10mm)
2. Find the actual size of the sampleI/AM I=10mm M=600A=?
A=10mm/600 = 0.0167mm =16.67 µm
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Your Turn!