DS-13009AL2013-12903

Lumi-Phos HRP (PS-atto) is a proprietary acridan basedchemiluminescent substrate for ELISA detection ofhorseradish peroxidase (HRP) conjugated molecules.

Lumi-Phos HRP couples a broad dynamic range with rapidgeneration of sustained luminescence.

Lumi-Phos HRPELISA HRP Substrate

Chemistry that brings meaning to light.

CH3S S(CH2)3O(CH2)3SO3NaS(CH2)3O(CH2)3SO3Na

N

CH2Ph

O

HRP + Light

O

O

N

CH2Ph

N

CH2Ph

CH3S

Reaction Mechanism

Y Excellent sensitivity – less than 5 pg/mL of target protein in plate based ELISA

Y Rapid peak intensity – reduced assay time and increased throughput

Y Sustained luminescence – timing is less critical

Y Temperature insensitive – analytical results areminsensitive to temperatures from 22°C - 35°Creducing the need for precise temperature control

5

3

4

2

1

0

-1-21 -20 -19 -18 -17 -16 -15

Log (moles HRP)

Log

(S-B

)

Figure 1. Lumi-Phos HRP couples a broad dynamic range with rapid generation of sustainedluminescence and superior storage stability to provide unmatched convenience to the user.

Excellent Sensitivity

1800

1500

1200

900

600

300

00 1 3 42 5

Time (min)

Inte

nsity

(RLU

s)

Addition of Substrate to Enzyme

Rapid Peak Intensity

Figure 2. Lumi-Phos HRP reaches a sustained maximum light-output peak intensity withinseconds of substrate addition. Signal intensities can be read at any time along the profile toproduce linear calibration curves.

22900 W. 8 Mile Road • Southfield MI 48033 USA • Telephone: 248 351-5600 or 877 LUMIGEN • Facsimile: 248 351-0518 • www.LUMIGEN.com

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Please visit www.LUMIGEN.com or contact LUMIGEN to request a quote.

Product Specifications

Enzyme Horseradish Peroxidase (HRP)

Application ELISA

Sensitivity Low picogram to femtogram

Ordering Information

Description Catalog Number

Lumi-Phos HRP (100 mL) PSA-100

Lumi-Phos HRP (1 L) PSA-1000