Live Imaging and Other Capabilitiesof the Bioscience Imaging Facility (BIF)

The BIF provides the instruments and expertise needed to visualize molecules in preparations ranging from single cells to entire animals.

• Project specific instrument training & advice

• Consultation on sample preparation, image processing, and data analysis

• Charge-back rates; Collaborations are encouraged

The Bioscience Imaging Facility

• High NA oil objectives 10x, 20x, 40x, 60x• Seven visible laser lines 446, 458, 488, 514, 561, 640 nm• Four channels or 32-bin spectral detection• Resonant scanner*• Piezo stage*• Live imaging chamber*

*Ideal for live imaging monolayer cell cultures

Rotate and Adj

• High NA oil objectives 10x, 20x, 40x, 60x• Seven visible laser lines 446, 458, 488, 514, 561, 640 nm• Four channel detection

• High NA oil objectives 10x, 20x, 40x, 60x

• Seven visible laser lines 446, 458, 488, 514, 561, 640 nm

• Four channels or 32-bin spectral detection• Resonant scanner & Piezo stage*• Live imaging chamber*

*Ideal for live imaging monolayer cell cultures

• High NA oil objectives 10x, 20x, 40x, 60x

• Seven visible laser lines 405, 458, 488, 514, 561, 640 nm

• Four channel detection

Nikon A1R

Nikon C1+

Confocal Microscopy

Resonant Galvanometer Piezoelectric z-stage

High Speed Image Acquisition

Spectral Acquisition

--Document spectra--Remove autofluorescence--Spectral FRET--Create virtual channels

T(λ) = C1•R1(λ) + C2•R2(λ)

Other Uses:

Spectral Unmixing

Fluorophoresin sample

Spectral Detector

• LWD (500 um) water 40x objective*• Mai Tai DeepSee tunable IR laser*• Four channel non-descanned detection*• Resonant scanner*• Live imaging chamber*• Confocal option Two laser lines (405, 488); four channel detection.

*Ideal for live imaging deep into living tissues

Nikon A1R_MP

Multiphoton Microscopy

• Phase and PlanFluor objectives* • DS-Ri1 color camera (standard)• Photometrics QuantEM CCD (optional)*• Live imaging chamber*

*Ideal for high sensitivity or high frame rate imaging of monolayer cell cultures

Nikon Ti-S

Wide-field Microscopy

Microscopic ImagesJuan Martinez, Mark HallBiochemistry

Pablo ZavattieriCivil Engineering

Shawn Liu, Xiaoqi LinBiology

Aaron TaylorBindley

• Cooled CCD• Range of excitation and emission filters Spans the 450-770 nm

• Isofluorane anesthesia provided

*Ideal for collecting signal from within an organism

IVIS Lumina II

In vivo Optical Imaging

In vivo Optical Images

Cansu Cimen, Tim RatliffComparative Pathobiology

Dan SzymanskiAgronomy

• Combined SPECT and x-ray CT Enables SPECT and CT image registration

• Multi-pinhole collimators High resolution imaging (350 um voxels) High sensitivity imaging (kBeq source)

• Data binning relative to biological rhythms Reduces blur

• List mode acquisition and statistical 3-D image reconstruction algorithm. Improves image quality

MiLabs U-SPECT-II/CT

Micro SPECT / CT Imaging

Principles of microSPECT

Principles of Statistical Reconstruction

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Object Space

Projection Space

Micro SPECT / CT Images

Xin Lu, Shuang LiuHealth Sciences

Charity Wayua, Phil LowChemistry

Live Imaging Accessories

For more information:Email: abtaylor@purdue.eduWeb: http://www.purdue.edu/discoverypark/bioscience/facilities/imaging/index.php

Thank You!