linkage chromosome mapping by recombination meiosis is the basis of transmission genetics the...

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Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes) generates data that are a useful tool for making linkage maps Linkage maps assist in understanding evolution, synteny, and selection response

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Page 1: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

Linkage

Chromosome mapping by recombination

Meiosis is the basis of transmission genetics

The recombination that occurs during meiosis (in heterozygotes) generates data that are a useful tool for making linkage maps

Linkage maps assist in understanding evolution, synteny,

and selection response

Page 2: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

F1

Parent 1 Parent 2

Populations for Linkage Analysis

Page 3: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

Doubled HaploidsParent 1 Parent 2

F1

Gametes

Page 4: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

×

P1 P2P2

Independent8 parental: 8 non-parental

Two loci:-Spike color- Plant height

Linked14 parental: 2 non-parental

What is Linkage?

Page 5: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)
Page 6: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

Linkage

• The association of two or more phenotypic characters in inheritance because the genes controlling these characters are located in the same chromosome.

• The closer the gene locations, the stronger the association

• Genes carried by the same chromosome are members of the same linkage group

• The number of linkage groups corresponds, therefore, to the basic number of chromosomes in the organism in question.

Page 7: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

Chromosomes

Plant 2n = _X = _ Genes

Arabidposis thaliana 2n = 2x = 10 27,000

Fragaria vesca 2n = 2x = 14 35,000

Theobroma cacao 2n = 2x = 20 29,000

Zea mays 2n = 2x = 20 40,000

Page 8: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

Linkage

• Crossing over refers to the physical exchange between homologous chromosomes. Meiotic crossing over is a potent source of genetic variability.

• Recombination is the genetic result of crossing over and is detected by new combinations of alleles at two or more loci.

Page 9: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

Prophase I (5 stages)1.  Leptonema: Longitudinal duality of chromosomes not discernible.

2.  Zygonema: Pairing of homologous chromosomes. Formation of synaptonemal complex and zygotene DNA synthesis.

3.  Pachynema: Pairing persists: synaptonemal complex + crossovers. Bivalent = 2 homologous chromosomes = 2 sets of 2 chromatids. Crossing over occurs (chiasma; chiasmata).

4.  Diplonema: Synaptonemal complex dissolves; visualize longitudinal duality.

5.  Diakinesis: Continued bivalent contraction. 

Meiosis

Page 10: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

How do nonsister chromatids ensure that crossing over between them will occur without the loss or gain of a single nucleotide? One plausible mechanism for which there is considerable laboratory evidence postulates the following events.

www.accessexcellence.org

Crossing Over

Page 11: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

Linkage

Complete: Pairs of loci are so close together that crossing over rarely occurs and recombinant types are generally not recovered.

0 10

0 crossovers between loci 1,2,3 and 10 crossovers between the cluster of loci 1/2/3 and locus 100 (in a population of 100 individuals)

Partial: Pairs of loci are sufficiently far apart that some recombinant types are recovered. The "distance" between genes ranges from a few percent recombination to 50% recombination.

1,2,3……..100

Page 12: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

Linkage

Terms describing the allelic condition at linked loci

cis aka coupling

trans aka repulsion

A……..B

a……..b

A……..b

a……..B

Page 13: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

Linkage

At Pachynema, there can be breakage of chromatids, followed by their fusion with sister chromatids, or with non-sister chromatids. As long as orientation corresponds, reciprocal exchanges between sister chromatids will not be detected and will not lead to genetic variability.

Page 14: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

Keys points in non-sister chromatid exchange:

• Crossing over does not involve loss or addition of chromatin

• Only two chromatids are involved in any single crossover event

• There may be multiple crossovers between non-sister chromatids

• Any combination of crossover configurations can occur, and the outcome of such configurations can be radically different

Linkage

Page 15: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

Linkage

Factors affecting meiotic crossing over Sex chromosomes

Page 16: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

Dioecy

Evolution of sex chromosomes from autosomes

• Accumulation of sex-determining genes on a single chromosome with no homolog prevents recombination between sex-determining genes

Linkage

Page 17: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

Linkage

Factors affecting meiotic crossing over

Position on chromosome

Page 18: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

Linkage Types of Crossovers

Single crossover 2-strand double crossover 3-strand double crossover 4-strand double crossover

Page 19: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

Oregon Wolfe Barley Map187 classical loci

BCD14340DsT-667Act8A12RbgMD17MWG837B22scind00046ABC165C25

Bmac039928GBM100730BCD09836GBM104249Bmag021159BG36994064ABC16079

JS10C109

MWG706A119KFP170126

MWG2028150ABC261151KFP257B152WMC1E8160MWG912162ABG387Ascssr04163166

1H

ABG0580scind026221ABG00813scssr1022641scssr0775945GBM106647Pox50scssr0338162Hot168Ebmac0684scssr0223669

scssr0033472ABG35675GBM102377scsnp0334390vrs196Bmag0125102DsT-41105MWG503110GBM1062111KFP203112MWG882A115ABG072133Ebmc0415150cnx1152Zeo1161GBM1019173Aglu5Aglu4176

MWG720179GBM1012185MWG949Ascssr08447193

2H

GBM10740MWG798B6Dst-279BCD70612

alm37Bmac020941ABC32545DsT-6748scssr2569163ABG37765Bmag022573

ABG49998

scsnp00940123

ABG004147

scind02281160MWG883165

HVM62183ABC805191

ABC172202

3H

MWG6340MWG07720HVM4023CDO54229scsnp0164830CDO12231hvknox335ABC303scssr2056941

CDO79544DST-46scind03751scind04312a

50

Tef2scind0172852

GBM102060Bmag035361scind1045568scssr1407980ABG47281GBM105984KFP22193Ebmac070195MWG652B96GBM1048102Hsh113HVM67114KFP241.1117ABG601127

4H

scssr023060MWG6187ABC48312ABG61013

ABG39541scssr0250348NRG045A61scsnp0426062Ale63

ABC30291scind1699194scssr1533496scsnp06144100srh111

RSB001A135scsnp001771420SU-STS1148ABG003B153

Tef3184MWG877188

ABG496203

E10757Ascsnp02109224

ABG391228JS10B229ABC622236DsT-33239MWG602A253scssr03906255

5H

Bmac03160

MWG602B36JS10A48GBM102155GBM106867BG29929770HVM3173rob75scssr02093Bmag000976

ABG47487ABG38897scsnp21226103MWG820107

GBM1008134scind04312b142GBM1022145Bmac0040153DsT-32B157DsT-28170DsT-74171MWG798AMWG514173

6H

ABG7040

GBSS-I16AW98258024CDO475MWG08931

ABG38033

scsnp0046072ABC25573ABC165D74scssr1586487GBM103091MWG808DAK64294

scsnp00703103MWG2031104nud108lks2124ABC1024128Bmag0120136DsT-30137WG380B138ABC310B149Ris44151ABC253169ABG461A179WG380ADsT-69184

GBM1065191KFP255215ThA1218

7H

Page 20: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

Locus abbreviations and allelesVrs1 = V: V, vGBSS = W: W,wNud = N: N,nLKs2 = L: L,l

N L

N L

W

W

n l

n l

w

w

V

V

v

v

Linkage RelationshipsOregon Wolfe Barley Parents

OWB Dominant2-row; normal starch; hulled grain; long awnsVV WW NN LL

OWB Recessive6-row; waxy starch; naked grain; short awnsvv ww nn ll

Page 21: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

N L

N L

W

W

n l

n l

w

w

V

V

v

v

Produce Recombinant2-row; waxy starch; hulled grain; short awn

OWB Dominant2-row; normal starch; hulled grain; long awnsVV WW NN LL

OWB Recessive6-row; waxy starch; naked grain; short awnsvv ww nn ll

x

n l

N L

w

W

v

V

F1

Page 22: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

nl

NL

w W

v V

nl

w

v

NL

W

V

nl

w

v

NL

W

V

Meiosis 1 & Crossing Over – Cell 1

After DNA replication

nl

w

v

nl

W

V

NL

w

v

NL

W

V

Pairing andcrossing over

nl

w

v

nl

W

V

NL

w

v

NL

W

V

Anaphase IPre-meiotic

Heterozygous parent

Ch 2

Ch 7

Page 23: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

Meiosis 2 & Crossing Over – Cell 1

n lwvn lWV

N LwvN LWV

Ch 2 Ch 7 Ch 2 Ch 7

Anaphase II

Ch 2 Ch 7

N LWVNon-Recombinant2row, non-waxy,

hulled, long awnN Lwv

Recombinant6row, waxy, hulled, long awn

n lWVRecombinant2row, non-waxy,

naked, short awn

n lwvNon-Recombinant6row, waxy, naked,

short awn

Page 24: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

nl

NL

w W

vV

nl

w

NL

W

nl

w

NL

W

vV vV

Meiosis 1 & Crossing Over – Cell 2

After DNA replication

nl

w

nl

W

NL

w

NL

W

vV vV

Pairing andcrossing over

nl

w

nl

W

NL

w

NL

W

vV vV

Anaphase IPre-meiotic

Heterozygous parent

Ch 2

Ch 7

Page 25: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

Meiosis 2 & Crossing Over – Cell 2

N LWvN LwV

n lWvn lwV

Ch 2 Ch 7 Ch 2 Ch 7

Anaphase II

Ch 2 Ch 7

Non-Recombinant2row, waxy, naked, short awn

n lwV

6row, non-waxy, naked, short awn

Recombinantn lWv

Recombinant2row, waxy, hulled, short awn N LwV

Non-Recombinant6row, non-waxy, hulled, long awn N LWv

Page 26: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

N LwV

N LWv

Non-Recombinant

Non-Recombinant

Recombinant

Recombinant

Doubled Haploid Phenotypes

2row, waxy, naked, short awn

6row, non-waxy, hulled, long awn

6row, non-waxy, naked, short awn

2row, waxy, hulled, short awn

n lwV

n lWv

N LwV

N LWv

n lwV

n lWv

n lWV

n lwv

N LWV

N Lwv

n lWV

n

l

wv

Non-Recombinant

Non-Recombinant

Recombinant

Recombinant

2row, non-waxy, hulled, long awn

6row, waxy, naked, short awn

6row, waxy, hulled, long awn

2row, non-waxy, naked, short awn

N LWV

N Lwv

Ch 2 Ch 7

Page 27: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

Gametes VW Vw vW vw

DH VVWW VVww vvWW vvww

Class Non-recombinant Recombinant Recombinant Non-

Recombinant

1 1 1 1

0.25 0.25 0.25 0.25

Dihybrid Ratio for Doubled Haploids

Page 28: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

Gametes VW Vw vW vw

DH VVWW VVww vvWW vvww

Non-recombinant Recombinant Recombinant Non-

Recombinant

0.25 0.25 0.25 0.25

Expected 20.5 20.5 20.5 20.5

Observed 20 15 25 22

(O-E)2/E 0.012 1.476 0.988 0.110

2[3] = 2.586; P=0.460; Null hypothesis of no linkage accepted

Independent Assortment – random alignment of chromosomes at Metaphase 1

Linkage Test – 2/6row v waxy/non

Page 29: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

Gametes WN Wn wN Wn

DH WWNN WWnn wwNN wwnn

Non-recombinant Recombinant Recombinant Non-

Recombinant

0.25 0.25 0.25 0.25

Expected 20.5 20.5 20.5 20.5

Observed 27 18 13 24

(O-E)2/E 2.061 0.305 2.744 0.598

2[3] = 5.707; P=0.128; Null hypothesis of no linkage accepted

Independent Assortment – random segregation at Metaphase 1

Linkage Test – waxy/non v hulled/naked

Page 30: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

Gametes WN Wn wN wn

DH WWNN WWnn wwNN wwnn

Non-recombinant Recombinant Recombinant Non-

Recombinant

0.25 0.25 0.25 0.25

Expected 20.5 20.5 20.5 20.5

Observed 26 19 20 17

(O-E)2/E 1.476 0.110 0.012 0.598

2[3] = 2.195; P=0.533; Null hypothesis of no linkage accepted

Independent Assortment – random segregation at Metaphase 1

Link Test – waxy/non v long/short awn

Page 31: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

Gametes NL Nl nL nl

DH NNLL NNll nnLL nnll

Non-recombinant Recombinant Recombinant Non-

Recombinant

0.25 0.25 0.25 0.25

Expected 20.5 20.5 20.5 20.5

Observed 37 3 9 33

(O-E)2/E 13.280 14.939 6.451 7.622

2[3] = 42.293; P=<0.001; Null hypothesis of no linkage rejected

The two loci tend to segregate together at Metaphase 1 and are therefore linked

Link Test – naked/hulled v long/short awn

Page 32: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

Gametes

4a b

Non Recombinant n4

3a B

Recombinant n3

2A b

Recombinant n2

1A B

Non Recombinant n1

Total N

Recombination frequency

N

n3n2r

Parent

21 43

A

B

A

B

a

b

a

b

Recombination

Page 33: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

Parentals (Non-recombinants) >> Recombinants = linkageLinkage is calculated as the total Recombinant types/ total population size

Nl (n2) = 3; nL (n3) = 9; N = 82

r = (n2+n3)/N; r= 12/82 = 14.6%

• N and L are approximately 14.6 map units apart • They are linked

“The association of two or more phenotypic characters in inheritance because the genes controlling these characters are located in the same chromosome”

Recombination Frequency

Page 34: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

Three Point Linkage Test

W N L

rWN=0.39 rNL=0.14

rWL=0.48

The recombination frequency over the interval W – L (rWL) is greater than either rWN or rNL therefore W and L are the two loci furthest apart and N is likely to lie between them. N is likely to be closer to L as rWN > rNL.

Page 35: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

• % recombination values are biased due to double crossovers• Double crossovers can give parental combinations of alleles and

therefore underestimate the % recombination at values of ~ 10% recombination and higher

• At values of ~ 10% recombination and less, double crossovers occur less often than expected

• % recombination values are not additive: the maximum of recombination is 50%

Therefore the centiMorgan – a computed value derived from the observed % recombination

Recombination and Map Distance

Page 36: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

Non-Additivity of recombination frequencies

A B C

rABrBC

rAC

The recombination frequency over the interval A – C (rAC) is less than the sum of rAB and rBC : rAC < rAB + rBC.This is because (rare) double recombination events (a recombination in both A - B and B - C) do not contribute to recombination between A and C.

Page 37: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

Distance (cM) Haldane =-0.5*LN(1-2*r)

Distance (cM) Kosambi=0.25*LN((1+2*r)/(1-2*r))

The centiMorgan is a unit of distance without a physical basis. This is readily apparent in organisms with large genomes, where the ratio of cM to base pairs can vary enormously across the genome

Map Distance Measures

Page 38: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

Mapping FunctionsMapping Function & Recombination

Page 39: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

Oregon Wolfe Barley Map187 classical loci

BCD14340DsT-667Act8A12RbgMD17MWG837B22scind00046ABC165C25Bmac039928GBM100730BCD09836GBM104249Bmag021159BG36994064ABC16079

JS10C109MWG706A119KFP170126MWG2028150ABC261151KFP257B152WMC1E8160MWG912162ABG387Ascssr04163166

1H

ABG0580scind026221ABG00813scssr1022641scssr0775945GBM106647Pox50

scssr0338162Hot168

Ebmac0684scssr0223669scssr0033472ABG35675GBM102377scsnp0334390vrs196

Bmag0125102DsT-41105MWG503110GBM1062111KFP203112MWG882A115ABG072133Ebmc0415150cnx1152Zeo1161

GBM1019173Aglu5Aglu4176

MWG720179GBM1012185MWG949Ascssr08447193

2H

GBM10740MWG798B6Dst-279BCD70612

alm37Bmac020941ABC32545DsT-6748

scssr2569163ABG37765Bmag022573

ABG49998

scsnp00940123

ABG004147scind02281160MWG883165

HVM62183ABC805191ABC172202

3H

MWG6340MWG07720HVM4023CDO54229scsnp0164830CDO12231hvknox335ABC303scssr2056941CDO79544DST-46

scind03751scind04312a

50

Tef2scind0172852GBM102060Bmag035361scind1045568scssr1407980ABG47281GBM105984KFP22193Ebmac070195MWG652B96GBM1048102Hsh113

HVM67114KFP241.1117ABG601127

4H

scssr023060MWG6187ABC48312ABG61013

ABG39541scssr0250348NRG045A61scsnp0426062

Ale63

ABC30291scind1699194scssr1533496scsnp06144100

srh111

RSB001A135scsnp001771420SU-STS1148ABG003B153

Tef3184MWG877188

ABG496203E10757Ascsnp02109224ABG391228JS10B229ABC622236DsT-33239

MWG602A253scssr03906255

5H

Bmac03160

MWG602B36JS10A48

GBM102155GBM106867BG29929770HVM3173rob75

scssr02093Bmag000976ABG47487ABG38897scsnp21226103MWG820107

GBM1008134scind04312b142GBM1022145Bmac0040153DsT-32B157DsT-28170DsT-74171

MWG798AMWG514173

6H

ABG7040GBSS-I16AW98258024CDO475MWG08931ABG38033

scsnp0046072ABC25573ABC165D74scssr1586487GBM103091MWG808DAK64294scsnp00703103MWG2031104nud108lks2124

ABC1024128Bmag0120136DsT-30137WG380B138ABC310B149Ris44151ABC253169ABG461A179WG380ADsT-69184

GBM1065191KFP255215ThA1218

7H

124 – 108 = 16 cM vs. 14.6% recombination

Page 40: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

Oregon Wolfe Barley Map187 classical + 555 pilot-OPA1 + 467 pilot-OPA2 = 1209 loci

BCD1434 2_027501_0460 1_131161_0410 DsT-6671_063681_101110Act8A112_122613RbgMD17MWG837B21scind00046 ABC165C25Bmac039928GBM1007291_0186 2_071232BCD098351_076047GBM1042 1_0814481_1498 1_1357 1_0238511_1266 2_0514521_0294571_1169 1_1036 1_1287 1_1301 2_06981_0938 1_0438 2_1312 Bmag0211 2_00472_0810 2_0757 2_1408 2_0912 2_10002_1357 2_0427 1_1336 1_1209

58

1_1107592_1217 1_1217 1_1359611_0293 1_0198 BG369940 1_1256622_1333 1_1444 1_0768651_0201 2_1463681_1049 1_0617691_0302 2_1131702_0642 2_1431721_1367 1_0002 1_106276ABC160771_0166 2_0696 1_0463791_1075 2_1176801_1267811_0279 1_0890 2_0121831_0466901_1463981_1144991_05351012_0475 1_1008 2_0769 JS10C 1_14391021_12771032_0754 2_01691072_0909 1_1173 MWG706A 2_1020 1_01112_0187 2_0613112

2_0780 KFP170 2_09211192_06251202_00211231_09051271_14091281_0854 1_03381302_11721351_03391361_1457137MWG2028 1_0207142ABC261 1_0808143KFP257B 1_06931441_09031462_03831481_1271149WMC1E8 2_1105151MWG912 1_1320 1_0041 1_15091542_0594155ABG387A 2_0840 scssr04163157

1H

1_0970 1_140702_1466 ABG05821_0718 scind0262232_141541_103051_1059 1_023011ABG008142_1423172_0724191_104021scssr10226 2_1261462_0593482_0368 scssr07759 1_0891 1_0307492_104950GBM1066 1_1304511_0847521_0342 2_1461 Pox 1_0837 2_03261_0919 1_1057 1_0525 1_0178 1_029654

1_1493 1_0084551_0715561_0486581_0204 1_1340 2_0281591_1505 1_0498622_1005 2_0688 1_0156 2_0674 1_1428641_1302 1_0638 scssr03381 1_1131 2_09291_042265

1_1522 1_1400672_0235 2_0602 1_0927 2_0748 2_02342_0387 1_0147 2_091770

2_0004 Hot1 2_0643 1_0558711_1155 1_0485 1_0474 1_0947 1_12882_0878 1_0883 Ebmac0684 2_0458 2_05001_0099 2_1434 2_0032 2_0039 1_12112_0181 1_0330 2_0417 2_0378 2_12861_1369 2_0196 scssr02236 1_0154 1_09482_1337

72

1_1384732_033574scssr00334751_0436 1_0640 1_0632762_0374 1_1430 2_1476 ABG356 2_04421_131677

2_1094 GBM1023 1_1347 1_1072791_1096821_0407 2_0833852_0667 2_0528861_140287scsnp03343931_1388941_0859 1_0786961_110098vrs1 1_1533 2_0340991_09361032_1351 1_09691042_0793 Bmag0125105DsT-41108MWG5031132_1007 1_1307 GBM1062 1_1285114KFP2031152_0923 MWG882A 1_03981181_1466 1_0900 2_15271191_13231241_10941272_0064 ABG072 1_0990 1_0989 1_01281_0263 2_1238 1_1043 2_0955134

1_0707 1_1236 1_0429 2_09891351_07391401_07801421_1365 2_1315 2_01411462_1406 1_0446 Ebmc0415 1_0109 1_14862_1459149

cnx11512_0215 2_1125 1_0065 1_0383 1_04722_1336155

1_1227157Zeo11602_0590 2_1181164GBM1019 1_0315 1_0566 1_10231722_0943173Aglu5 Aglu4176MWG720 1_09371792_0957180GBM1012 1_03291851_1050 1_00851872_14141892_1496190MWG949A scssr08447192

2H

2_0858 2_009002_079731_143461_1453 2_0953 2_025292_1027 1_1005 2_1398131_1310 2_0976 1_0886142_0172 2_1410202_0742 1_0565212_0982 GBM1074272_0552292_0794 1_0559 1_0691302_0968 2_0455 MWG798B 1_1237 2_1402321_0026 2_1268 Dst-27 2_0607 1_1527361_0081 1_0968 BCD706 1_0598392_1259 1_1117 2_1506502_1189561_1069 2_0264571_1102581_1387591_1511 alm 1_0233602_0866611_1265 1_0391621_1295 2_1062 2_1472 1_1482 1_12832_0591 1_1337 2_1435 1_1099 2_04392_0721 Bmac0209 1_0925 2_0486 1_11252_1411 1_1530 1_0784 2_0801 2_11472_0583 2_0408

64

1_0011 2_0444651_0850 ABC325 1_1429 2_0325 2_10642_0108 1_0678 1_0653 2_151168

DsT-67712_0863 1_1191722_0608 1_1342 1_1284 1_1152 2_1502732_1305761_0200 1_1394 1_1314772_0694 1_0452802_0124 2_0695 1_0683 2_0521 1_0609812_0362 1_0047 scssr25691 2_0597 2_11941_145484

2_0849 2_1163 1_1524 2_0115 ABG377861_1517902_0063 1_113893Bmag0225941_0583 2_06591031_0747 1_1021106ABG4991152_01301161_01841172_1083 2_13811182_1495 1_06621242_14931252_00091272_1081 2_0023 1_1392 2_11611282_12771321_0584136scsnp00940 1_06801371_13301432_09441442_1405 1_09181461_0821 1_0754 1_13381522_04131532_0369 1_1196 2_0662 1_1141 ABG0042_0343 1_0505158

2_0612 1_08421592_0527162scind022811692_0085 1_1328170MWG8831752_13861801_12971822_15311862_0421189HVM62 2_1345 1_14361902_1008 1_0935 1_0702 2_1237196ABC805 2_06051981_0893 1_0629 1_06811991_00142001_15162022_01762042_15002061_0343 1_0215 ABC172 2_1387208

3H

2_11170MWG63472_027491_1485161_049017MWG077241_0574 2_0557 HVM40 1_0223261_0221271_1199281_1136292_1458311_1175 1_0626 2_1385 1_1060 1_05622_042232

2_0109 2_1418 2_0680 2_0777 CDO542scsnp0164833

CDO122 1_0031341_0810 hvknox3 2_1389381_0171 1_0347412_0240 1_0371 2_0011 2_0180 1_006342scssr20569 ABC303 1_0048441_0668 CDO795 1_0793472_0939 1_1112 2_1490492_1303 2_1071 2_0269 2_0289 1_09461_0942 1_1332 2_0426 1_0667 2_08311_0756 1_0411 2_0853 1_1042 1_11902_0496 1_0607 1_0261 1_0577 2_10731_0492 2_1137 1_0432 2_1029 scind037512_1442 DST-46 scind04312a 2_0525

53

2_1481 2_0898 2_0363 2_0412 2_06101_0527 1_1108 1_0262 scind01728 1_08812_1213 1_0568 1_0426 1_1232 1_0637Tef2 1_0088

55

2_0151 2_1400 1_0086 2_0361 2_1010592_1296 1_1427 2_0453 1_1207 2_1273622_0820 GBM1020632_0723 1_0052 2_0924 1_0053 1_1431Bmag0353 2_0062 1_0639 2_009864

1_1070651_060666scind10455701_1513 2_1504 1_0467711_0309 2_0143 2_0207 2_1499 1_1319762_1332791_0170 scssr14079 1_150082ABG472 1_1526832_0197 1_1213 1_0670 1_072384GBM1059 2_0765 1_1398852_0732921_1168931_0785 KFP22194Ebmac070196MWG652B 2_0762 1_1292 1_0666 2_0838971_1139 2_0454 1_0334 2_111199GBM10481031_11941051_1233 2_0237 1_07121091_1235 2_07011102_0883 1_1066 2_10351122_1130 Hsh 1_0697113HVM67 2_02241142_07601151_0610 2_1450 2_0272 2_0007116KFP241.1 2_0932117ABG601127

4H

2_055302_12219scssr0230613MWG618202_053322ABC483 2_001024ABG610252_1365262_1065 2_0873331_0974342_0761352_0576391_0580432_0980 2_0845 2_1391471_1318 ABG395481_086449scssr02503 1_1432562_1401 1_1399 1_0856 2_0481 1_14622_1040 1_0157 1_0481 1_0240 2_14471_1469 2_0700 1_0177 1_0995 1_11982_0101 2_0697 2_1011 2_0461 1_02522_1116 1_0318 1_0913 2_1308 2_08412_0179 2_0524 2_1350

57

1_1260 1_1204582_0396 2_0059611_0264 2_1536 2_0105632_0306 1_1240 1_1151 1_0899 1_15121_1363 2_0501 2_0981 1_023764

1_1523661_0725 NRG045A 1_108967scsnp0426068Ale 2_1036692_0392 1_0203 2_0278 1_1438 1_12491_064172

1_0548792_0297801_0953812_0818 2_0330851_0305871_0183 2_1128 1_0127 2_1133 ABC302901_0140 scind16991 2_0246 2_0096 1_01671_063393

2_0736 1_1515 2_006694scssr15334962_118698scsnp06144 1_11351011_06741061_05241091_09301101_05781112_0097 2_0449 srh 1_1134 1_05181_1473 1_0771112

1_13501202_04031212_01341231_00241252_1321 1_0844 1_08341262_0805 1_0507 1_08711281_0477130RSB001A 1_1341131scsnp00177 1_12451390SU-STS11451_12981461_15071482_14221502_0045 2_0405 ABG003B1511_14561522_0300 1_1535 1_02281531_14721562_0551 1_0755167Tef31751_00041762_0731 2_0830 1_0104 2_06761772_0100 1_1441 1_0080 2_1462 MWG8771_0333 2_0388178

2_01041822_01861841_14971851_0582 ABG496 1_0902 1_0741 1_09011_0536 1_0385 2_0317192

2_0876 2_05601931_0273 1_1464 2_0646 2_1018 1_13251_0336195

2_03342072_0829 E10757A scsnp021092112_08262121_1216 ABG3912151_0769 JS10B 2_05462161_07782211_06002221_0254 1_1010 ABC622 1_14502231_0736 DsT-33 2_01892252_13932311_03102321_1364 2_11082351_0732 1_0020 1_1413237MWG602A2382_1356 scssr03906 2_0402 1_0322239

5H

2_02320Bmac0316 2_088122_088231_116642_0262 2_0294 2_12045MWG602B 1_0064351_0023 2_1246372_0315401_0774 JS10A 2_0745471_0799 1_0994 GBM1021 1_0939541_0427551_0129 1_1455 1_0061 GBM1068642_0936662_0983 1_0575 1_0461 1_0199 1_09101_0811 1_0539 1_0817 2_0707 BG2992971_0355

68

2_0675 1_1205 1_1171692_144970HVM31711_1104 2_0651 1_1353 1_0497 rob2_0656 2_1158 1_0962 2_1473 2_03812_0720 2_1014 2_0601

73

1_0591 2_0567 2_0785 1_1487 1_03232_0799 2_0572 1_1312 scssr02093 1_06591_0848 1_0749 2_0142 2_1216 1_09541_1153 1_1442 2_0854 2_0946 Bmag00091_1140

74

1_0278 1_0345 2_0835 1_1181 2_02022_1114 1_051375

1_0803821_0964 ABG474 2_0266841_1321 1_0189852_1298 2_1225 2_1293 2_0904 1_03922_0148 1_126190

2_0714 ABG388 1_0504932_0468942_0620 2_0892 1_0596 2_0673992_0577 2_0848 scsnp21226 1_1349 2_07841002_0488 MWG820 2_06821031_03311091_0185 1_12461102_1404 2_03461111_11471131_04001192_1025 1_02021202_09961221_0595 GBM1008 2_0972 1_09781261_1494 1_01391272_0379129scind04312b132GBM1022 2_00361362_0355 Bmac00401442_04481452_0558 DsT-32B1472_14771551_0645 1_0071 2_1455 2_1467 1_00511_0107157

1_1187 DsT-281581_0748 DsT-741612_0907 2_08681621_1488 2_02111631_1111 MWG514 MWG798A 1_0390165

6H

2_1419 1_003601_1132 ABG704 1_068211_0894 2_0201 1_012121_117961_103171_103592_0227122_075513GBSS-I151_1052182_105019AW982580 2_0495231_0965 CDO475 MWG089301_0451311_0232 2_0192 ABG380 2_0993322_1292391_0979401_0368451_1339472_1491482_1326 2_0150491_0772 2_0031522_011356scsnp0046060ABC255 1_040361ABC165D 1_0721621_0050642_0750 1_0657 2_1270652_0060 1_1028 1_1242702_1234 1_1257 1_1045 scssr15864 2_14921_1202 1_110375

1_0983 2_1167 2_088576GBM1030781_0083 1_1519801_1352 2_0879 2_1494 2_0828 MWG8081_0773 2_0200 1_0370 1_0698 2_13351_1291 1_0073 1_0268 1_0055 2_03141_0581 2_1319 2_1302 2_0893 1_0115DAK642 1_1145 2_1178 1_0924 2_0911

81

1_1029 1_1461881_0442 2_1330 1_0531 1_1091 scsnp007031_1445 2_0349 1_0673 2_089691

2_0083 1_0143 MWG2031 2_1409 2_077192nud952_1530 2_0685 1_0249 2_1501 1_1437962_1201 lks2111ABC10241142_08081152_0103 2_06111161_10511181_0169 2_1464120Bmag0120 2_08241221_0362 DsT-30 2_0406123WG380B1241_0563130ABC310B 1_11841342_0217 Ris44 1_12431372_03541411_1279 2_1229 2_12091481_0861 ABC2531521_0797159ABG461A 1_09731632_1280 1_0550 2_07531662_0414 1_1440 DsT-69 2_0452 WG380A2_1363 2_1223 1_0843167

2_06401681_01301701_0896 2_0962 1_1309 1_1275171GBM10651741_0378 KFP255198ThA1200

7H

111- 96 = 16 cM vs. 14.6% recombination

Page 41: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

94.2 – 81.6 = 12.6 cM vs. 14.6% recombinationcM < % recombination???

Recombination vs. Map Distance

Page 42: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

• Determine order of loci in the chromosome• Determine how likely it is that non-parental combinations

of alleles at linked loci will be obtained• Determine if trait relationships are due to linkage or

pleiotropy • Create complete linkage maps (average ~ 100

cM/chromosome) where # chromosomes (n level) = number of linkage groups

• Build a platform for cloning genes• A framework for whole genome sequences• Identify genes controlling quantitative traits

Value of Genetic Linkage Maps

Page 43: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

Visualise the consequences of recombination Graphical genotypes (haplotypes) of selected a mapping population

Graphical Genotypes

Page 44: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

• Linkage analysis is now an "automated" procedure Data on thousands, tens of thousands of loci

• Key issues in linkage map construction are locus order and distance

• The likelihood odds (LOD) score is a test statistic that is used to test the hypothesis that there is no linkage LOD of 3.00 is approximately equal to P ~ .001 LOD > 3 then one concludes that two loci are indeed linked.

Making Linkage Maps

Page 45: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

Locus ordering

6

)2)(1(

kkkntriplets

No. of locik

Possible orders

No. of triplets

2 1 0

3 3 1

5 60 10

10 1,814,400 120

20 1.22 X 1018 1,140

40 4.08 X 1047 9,880

Number of orders among k loci

Number of triplets among k loci

2

)1)(2)(3)...(2)(1(

2

!

kkkknk

Page 46: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

Oregon Wolfe Barley Map187 classical loci

BCD14340DsT-667Act8A12RbgMD17MWG837B22scind00046ABC165C25

Bmac039928GBM100730BCD09836GBM104249Bmag021159BG36994064ABC16079

JS10C109

MWG706A119KFP170126

MWG2028150ABC261151KFP257B152WMC1E8160MWG912162ABG387Ascssr04163166

1H

ABG0580scind026221ABG00813scssr1022641scssr0775945GBM106647Pox50scssr0338162Hot168Ebmac0684scssr0223669

scssr0033472ABG35675GBM102377scsnp0334390vrs196Bmag0125102DsT-41105MWG503110GBM1062111KFP203112MWG882A115ABG072133Ebmc0415150cnx1152Zeo1161GBM1019173Aglu5Aglu4176

MWG720179GBM1012185MWG949Ascssr08447193

2H

GBM10740MWG798B6Dst-279BCD70612

alm37Bmac020941ABC32545DsT-6748scssr2569163ABG37765Bmag022573

ABG49998

scsnp00940123

ABG004147

scind02281160MWG883165

HVM62183ABC805191

ABC172202

3H

MWG6340MWG07720HVM4023CDO54229scsnp0164830CDO12231hvknox335ABC303scssr2056941

CDO79544DST-46scind03751scind04312a

50

Tef2scind0172852

GBM102060Bmag035361scind1045568scssr1407980ABG47281GBM105984KFP22193Ebmac070195MWG652B96GBM1048102Hsh113HVM67114KFP241.1117ABG601127

4H

scssr023060MWG6187ABC48312ABG61013

ABG39541scssr0250348NRG045A61scsnp0426062Ale63

ABC30291scind1699194scssr1533496scsnp06144100srh111

RSB001A135scsnp001771420SU-STS1148ABG003B153

Tef3184MWG877188

ABG496203

E10757Ascsnp02109224

ABG391228JS10B229ABC622236DsT-33239MWG602A253scssr03906255

5H

Bmac03160

MWG602B36JS10A48GBM102155GBM106867BG29929770HVM3173rob75scssr02093Bmag000976

ABG47487ABG38897scsnp21226103MWG820107

GBM1008134scind04312b142GBM1022145Bmac0040153DsT-32B157DsT-28170DsT-74171MWG798AMWG514173

6H

ABG7040

GBSS-I16AW98258024CDO475MWG08931

ABG38033

scsnp0046072ABC25573ABC165D74scssr1586487GBM103091MWG808DAK64294

scsnp00703103MWG2031104nud108lks2124ABC1024128Bmag0120136DsT-30137WG380B138ABC310B149Ris44151ABC253169ABG461A179WG380ADsT-69184

GBM1065191KFP255215ThA1218

7H

Page 47: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

Oregon Wolfe Barley Map187 classical + 722 DArT = 909 loci

bPb-63180BCD1434 bPb-71121DsT-66 bPb-01818bPb-1318 Act8A bPb-9608bPb-7043 bPb-713712

[220835] [222208] [105102]RbgMD18

[222789]19bPb-7889 MWG837B23scind00046 ABC165C26[223986]27Bmac039929GBM100730[105118] bPb-216634BCD098 [221879]36bPb-4813 bPb-3217 bPb-941843[104834] [104921]48[221425] GBM104251bPb-5672 bPb-1231 bPb-1398bPb-9388 bPb-8662 bPb-1535bPb-1586

54

bPb-1604 bPb-0482 bPb-5164bPb-2175 [222328] bPb-0357[221108]

55

[223055] [221321] bPb-9360Bmag0211 bPb-057961

bPb-3382 BG369940 bPb-1791bPb-119365

bPb-1723 bPb-900571bPb-1133 [222086] bPb-818375bPb-3389 bPb-533980ABC16081bPb-6117 bPb-871383[224235]88bPb-847799bPb-7963 JS10C107bPb-2007111bPb-9180 bPb-1419 MWG706A[221687] bPb-7429117

[223108] KFP170124bPb-9108 [221039]125bPb-2063 bPb-5198 bPb-5014129[221902]135MWG2028147bPb-5090 ABC261148bPb-9552 KFP257B bPb-6065149bPb-6502152WMC1E8157MWG912159ABG387A bPb-2260 bPb-3756scssr04163 bPb-1487 bPb-5550163

1H

[223556]0bPb-7151 bPb-5191 bPb-3608bPb-3598 bPb-0485 bPb-0516[222367] bPb-7892 bPb-0611bPb-8959 [220862]

1

ABG0583[222211] scind026224bPb-7969 bPb-0205 bPb-0471bPb-5033 bPb-5900 [223937]7

[221144] bPb-7557 bPb-7024[223539] bPb-5688 bPb-5519bPb-0615

9

[223312]14bPb-6897 bPb-8399 [220974]bPb-4295 bPb-9220 bPb-0715ABG008 bPb-1708 bPb-5444bPb-4481 [221686] bPb-6128

16

bPb-8374 bPb-4715 bPb-2355[222296] bPb-2108 [104949]bPb-2782 bPb-6494 [222714]bPb-6879 [221518] bPb-8986

18

bPb-829220bPb-4261 scssr10226 bPb-9682bPb-487548

bPb-3190 bPb-7906 bPb-2501scssr0775951

GBM106654bPb-0722 Pox bPb-223057bPb-9925 bPb-505765bPb-0220 [223952]67[221628] scssr03381 [222438]68bPb-3056 bPb-6438 bPb-688173Hot174bPb-1836 scssr02236 [220925][220842] [222433] Ebmac068475

scssr0033478ABG35681bPb-6437 GBM1023 bPb-4837bPb-7256 bPb-088583

bPb-5440 bPb-6088 bPb-4040[222771] bPb-975485

bPb-8100 bPb-724388[222968] scsnp0334396vrs1103Bmag0125109DsT-41112MWG503117bPb-6194 bPb-1926 bPb-3563bPb-4835 GBM1062 bPb-7991118

KFP203119MWG882A123bPb-3653 bPb-8737 bPb-4577bPb-1772128

bPb-6822131bPb-8274 bPb-3870 ABG072bPb-2481 [223776]138

bPb-1266 bPb-8949148bPb-6688 bPb-7992 [221435]bPb-0541151

Ebmc0415 [222960]153cnx1 bPb-5412 bPb-2971155[222464]157bPb-3858160[221983] bPb-1505163Zeo1164[223553]176[221407] bPb-1986 bPb-1566bPb-8530 bPb-5460 bPb-5619bPb-1184 bPb-7208 bPb-4092bPb-1815 bPb-0303 GBM1019

178

Aglu4 Aglu5181MWG720184bPb-1181 bPb-4601 bPb-7212bPb-4232 bPb-1611188

GBM1012190bPb-8698 [223337] bPb-3102bPb-1085 bPb-6296 bPb-9419bPb-6048 bPb-0305

192

MWG949A scssr08447198

2H

bPb-40220[221259]2bPb-4895 bPb-2553 [223116]bPb-3815 [224140]3

bPb-12649bPb-368911bPb-994513[221362]16[223396] [220882] [221142][223877]17

bPb-974520bPb-0654 [221551] [221552]bPb-744821

bPb-0548 bPb-3865 bPb-0663bPb-3229 bPb-482423

GBM107427[221274] MWG798B [220909]32bPb-9903 [105024] bPb-5289bPb-9961 bPb-292933

bPb-3030 Dst-2736bPb-2910 [220886] [221166]bPb-6275 [222615] [222320]bPb-5892 bPb-7938 bPb-2993[222411] BCD706 bPb-2324bPb-9129

39

bPb-0158 bPb-333243bPb-440757[224015] bPb-0068 [223843]bPb-677159

alm60Bmac0209 bPb-866665bPb-649167ABC32568DsT-6771bPb-475772bPb-0040 bPb-6923 bPb-380573bPb-8410 bPb-0079 bPb-331778bPb-7872 [223370] scssr2569185ABG37787Bmag022595ABG499120bPb-6722121[221404]122[223016]133bPb-9336134scsnp00940143bPb-4209 bPb-3630144bPb-1961 bPb-9110 bPb-1579bPb-7695150

[223788]152bPb-4386 bPb-7785153ABG004164scind02281176bPb-5796 bPb-8506 bPb-8894177bPb-4830179MWG883182bPb-2737 bPb-5129 bPb-8021bPb-7833 bPb-0049 bPb-3109187

bPb-4739 bPb-2420 bPb-1253bPb-4748 bPb-3899 bPb-6228bPb-3843

190

bPb-0789192bPb-7413 [222583]193bPb-4456 bPb-6631 bPb-0302bPb-1829 HVM62197

bPb-0200200ABC805205bPb-4387 bPb-4169207bPb-1411209[222092] [222593]210bPb-0164212bPb-3295 bPb-8962 ABC172[221355] bPb-7120 bPb-5346[223314] [221492] [221662]bPb-7724 bPb-0136 bPb-5256

217

[221563]219

3H

bPb-7534 bPb-6938 bPb-94060MWG6346MWG077 [222436]25HVM40 [104902]27[222526]33CDO54234scsnp01648 CDO12235[222439]38hvknox340[223373] bPb-6404 bPb-661143ABC303 scssr2056945CDO79549bPb-9504 DST-46 scind03751[223751] [220846] scind04312a[222294]

54

Tef2 scind01728 [105086]57bPb-6949 bPb-6872 bPb-9907bPb-230563

bPb-4290 bPb-6259 bPb-7987GBM102064

[224001] Bmag035366[221608] scind10455 bPb-421472bPb-8013 bPb-009875bPb-355678scssr1407984bPb-9998 ABG472 bPb-6096bPb-7719 bPb-373985

bPb-0018 GBM1059 bPb-1999bPb-0610 bPb-475087

KFP221 bPb-524596Ebmac070199MWG652B100bPb-9859 bPb-1166 [223032]GBM1048105

bPb-2677112bPb-1040 [220875]113Hsh [223550]116HVM67117bPb-3468 [223334]119KFP241.1120bPb-5265 bPb-9668 ABG601129

4H

bPb-01700bPb-6051 bPb-64852scssr0230616MWG61823ABC48327ABG61028bPb-287229bPb-0091 bPb-7407 bPb-3830bPb-8072 bPb-2460 bPb-1807bPb-6568 bPb-0351

36

bPb-5166 bPb-226641bPb-053642ABG39551bPb-4273 bPb-8589 bPb-4135bPb-227352

scssr0250359bPb-8675 [222988] bPb-5584[221402] bPb-4210 bPb-8929bPb-0786 bPb-1046 [223293]bPb-0949

60

bPb-5727 bPb-7881 bPb-5506bPb-276261

bPb-793367[221802] bPb-050368NRG045A70bPb-7627 scsnp04260 [223210]bPb-626071

bPb-8987 [224132] Ale73[222177] [223146] [223812]76bPb-7763 [221220] bPb-0686bPb-9618 bPb-4067 bPb-214789

ABC30296scind1699198scssr15334101scsnp06144105srh116[105044] bPb-9032 bPb-2869bPb-5532117

bPb-5596 bPb-7395 RSB001A136[221888] scsnp00177 [222196]1460SU-STS1152bPb-0710 bPb-6135156ABG003B [104960]158bPb-2960 [221423] bPb-4084[223247]175

bPb-5845 [222215] bPb-6578182Tef3183MWG877186[223182]188[105067]189ABG496200bPb-9147 bPb-6195 [222383]216E10757A scsnp02109220ABG391 bPb-3138 [221131]bPb-0618224

JS10B225bPb-6179 bPb-4595 bPb-0835228[104899]229[223803] [222906] bPb-4621[222776] [223339] bPb-0877[224041]

231

ABC622232bPb-9132 bPb-7421 DsT-33234bPb-9868237bPb-8754241[222075] bPb-6239 bPb-1217244bPb-3973 [221531] MWG602AbPb-7008 bPb-2419247

bPb-6124 bPb-5766 scssr03906bPb-4725 bPb-7292 bPb-7360bPb-9244

249

[221535]250

5H

[223608] bPb-37800[222141] [223456] bPb-7030bPb-7323 Bmac03162

bPb-97683bPb-6511 bPb-8275 [221458]bPb-9075 [223070] bPb-3532bPb-1001

5

bPb-7193 bPb-38077[221961] bPb-0572 bPb-4778[222053] [222316] bPb-5498bPb-3433 bPb-6311

20

bPb-8492 [224154]26MWG602B30[223325] [223374] bPb-716531bPb-5252 bPb-6661 bPb-974935[222286] [221152]38[220998] [223808]40JS10A bPb-392141GBM102148bPb-3927 bPb-6002 [221169][223386] bPb-059749

[220812] bPb-732450[221710]56GBM1068 [223273]59[220818] bPb-537960bPb-6142 BG299297 bPb-146662[223115] bPb-5270 bPb-259263HVM3165bPb-3487 bPb-6567 bPb-1666rob bPb-911467

[223671] [222912] [223207]Bmag0009 bPb-2593 scssr02093[223262] [224033] [223825][223845] [223002] [224253][222899] [222814] [221391][223658] [223028]

68

bPb-1657 bPb-9702 [222484][222527] [221245] [221874]bPb-0019 bPb-5196

69

bPb-9051 bPb-9082 bPb-983570bPb-5517 bPb-4229 bPb-947976bPb-6721 bPb-8347 bPb-4409bPb-4369 ABG474 bPb-323078

bPb-3068 bPb-1256 [224127]79ABG38887[223211]93scsnp2122694MWG82098[222895]114GBM1008122[220914] [221783]123bPb-9063 bPb-6477127scind04312b129GBM1022132bPb-3470133Bmac0040140[221088] [221063] bPb-5587141DsT-32B143bPb-7446 bPb-9413154DsT-28155bPb-5515156DsT-74157bPb-3760 bPb-2863 bPb-6677bPb-2940159

bPb-2304 MWG514 bPb-1621bPb-0403 bPb-7146 bPb-1029162

bPb-2410 [223186] bPb-9890[222800] bPb-9817163

bPb-0649 MWG798A bPb-4626164[220848]165

6H

ABG7040bPb-3732 [223392] bPb-8272[223152] bPb-0844 bPb-8084bPb-4457 bPb-0398 bPb-7559bPb-7349

2

[221346] [222156] [224158]bPb-4445 [221162]6

bPb-25958[223056] bPb-5816 [221786]9bPb-9202 bPb-589710bPb-4634 [222301]11GBSS-I14bPb-6453 bPb-372717bPb-426621AW98258022[222485]24MWG089 CDO475 bPb-866029bPb-5074 bPb-5494 bPb-9601bPb-5852 bPb-0678 bPb-2533bPb-1360 ABG380 bPb-6156[221832] bPb-0324 bPb-8939

31

bPb-867461scsnp0046066ABC25567ABC165D [220928] [221740]68bPb-1952 bPb-7952 bPb-3561bPb-421974

bPb-8051 bPb-144779[221655] bPb-1093 [222063]scssr15864 [221264] bPb-545880

[222546] [104913]81GBM103084bPb-5126 bPb-2097 bPb-8956DAK642 bPb-3227 bPb-2188bPb-6975 MWG808 bPb-5599bPb-2379 bPb-7915 bPb-8396bPb-1770

87

bPb-982589scsnp0070396MWG203197[221574]98nud bPb-8439 bPb-6233100bPb-5296 bPb-7399 bPb-2561bPb-1847 bPb-6386 bPb-9912112

lks2116ABC1024119bPb-1079 [222171]121[220920]122bPb-4191126Bmag0120127DsT-30128WG380B129bPb-0027 bPb-9908 bPb-2433135bPb-4272 [105081]136ABC310B139bPb-8498140Ris44 bPb-4389 bPb-6186141[221079]155ABC253158bPb-7739165[222159] bPb-0758 bPb-8644bPb-6167 bPb-5923 bPb-0889bPb-4394 bPb-0917 bPb-1556ABG461A

168

bPb-3980 bPb-1631 bPb-7983[220949] bPb-0981172

[222528] bPb-8833 WG380ADsT-69173

bPb-2121 [223928] bPb-3232bPb-5898 bPb-9948176

[221299]177GBM1065179[223404]181bPb-0328 bPb-9884 KFP255203ThA1206

7H

Page 48: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

Oregon Wolfe Barley Map187 classical + 555 pilot-OPA1 + 467 pilot-OPA2 = 1209 loci

BCD1434 2_027501_0460 1_131161_0410 DsT-6671_063681_101110Act8A112_122613RbgMD17MWG837B21scind00046 ABC165C25Bmac039928GBM1007291_0186 2_071232BCD098351_076047GBM1042 1_0814481_1498 1_1357 1_0238511_1266 2_0514521_0294571_1169 1_1036 1_1287 1_1301 2_06981_0938 1_0438 2_1312 Bmag0211 2_00472_0810 2_0757 2_1408 2_0912 2_10002_1357 2_0427 1_1336 1_1209

58

1_1107592_1217 1_1217 1_1359611_0293 1_0198 BG369940 1_1256622_1333 1_1444 1_0768651_0201 2_1463681_1049 1_0617691_0302 2_1131702_0642 2_1431721_1367 1_0002 1_106276ABC160771_0166 2_0696 1_0463791_1075 2_1176801_1267811_0279 1_0890 2_0121831_0466901_1463981_1144991_05351012_0475 1_1008 2_0769 JS10C 1_14391021_12771032_0754 2_01691072_0909 1_1173 MWG706A 2_1020 1_01112_0187 2_0613112

2_0780 KFP170 2_09211192_06251202_00211231_09051271_14091281_0854 1_03381302_11721351_03391361_1457137MWG2028 1_0207142ABC261 1_0808143KFP257B 1_06931441_09031462_03831481_1271149WMC1E8 2_1105151MWG912 1_1320 1_0041 1_15091542_0594155ABG387A 2_0840 scssr04163157

1H

1_0970 1_140702_1466 ABG05821_0718 scind0262232_141541_103051_1059 1_023011ABG008142_1423172_0724191_104021scssr10226 2_1261462_0593482_0368 scssr07759 1_0891 1_0307492_104950GBM1066 1_1304511_0847521_0342 2_1461 Pox 1_0837 2_03261_0919 1_1057 1_0525 1_0178 1_029654

1_1493 1_0084551_0715561_0486581_0204 1_1340 2_0281591_1505 1_0498622_1005 2_0688 1_0156 2_0674 1_1428641_1302 1_0638 scssr03381 1_1131 2_09291_042265

1_1522 1_1400672_0235 2_0602 1_0927 2_0748 2_02342_0387 1_0147 2_091770

2_0004 Hot1 2_0643 1_0558711_1155 1_0485 1_0474 1_0947 1_12882_0878 1_0883 Ebmac0684 2_0458 2_05001_0099 2_1434 2_0032 2_0039 1_12112_0181 1_0330 2_0417 2_0378 2_12861_1369 2_0196 scssr02236 1_0154 1_09482_1337

72

1_1384732_033574scssr00334751_0436 1_0640 1_0632762_0374 1_1430 2_1476 ABG356 2_04421_131677

2_1094 GBM1023 1_1347 1_1072791_1096821_0407 2_0833852_0667 2_0528861_140287scsnp03343931_1388941_0859 1_0786961_110098vrs1 1_1533 2_0340991_09361032_1351 1_09691042_0793 Bmag0125105DsT-41108MWG5031132_1007 1_1307 GBM1062 1_1285114KFP2031152_0923 MWG882A 1_03981181_1466 1_0900 2_15271191_13231241_10941272_0064 ABG072 1_0990 1_0989 1_01281_0263 2_1238 1_1043 2_0955134

1_0707 1_1236 1_0429 2_09891351_07391401_07801421_1365 2_1315 2_01411462_1406 1_0446 Ebmc0415 1_0109 1_14862_1459149

cnx11512_0215 2_1125 1_0065 1_0383 1_04722_1336155

1_1227157Zeo11602_0590 2_1181164GBM1019 1_0315 1_0566 1_10231722_0943173Aglu5 Aglu4176MWG720 1_09371792_0957180GBM1012 1_03291851_1050 1_00851872_14141892_1496190MWG949A scssr08447192

2H

2_0858 2_009002_079731_143461_1453 2_0953 2_025292_1027 1_1005 2_1398131_1310 2_0976 1_0886142_0172 2_1410202_0742 1_0565212_0982 GBM1074272_0552292_0794 1_0559 1_0691302_0968 2_0455 MWG798B 1_1237 2_1402321_0026 2_1268 Dst-27 2_0607 1_1527361_0081 1_0968 BCD706 1_0598392_1259 1_1117 2_1506502_1189561_1069 2_0264571_1102581_1387591_1511 alm 1_0233602_0866611_1265 1_0391621_1295 2_1062 2_1472 1_1482 1_12832_0591 1_1337 2_1435 1_1099 2_04392_0721 Bmac0209 1_0925 2_0486 1_11252_1411 1_1530 1_0784 2_0801 2_11472_0583 2_0408

64

1_0011 2_0444651_0850 ABC325 1_1429 2_0325 2_10642_0108 1_0678 1_0653 2_151168

DsT-67712_0863 1_1191722_0608 1_1342 1_1284 1_1152 2_1502732_1305761_0200 1_1394 1_1314772_0694 1_0452802_0124 2_0695 1_0683 2_0521 1_0609812_0362 1_0047 scssr25691 2_0597 2_11941_145484

2_0849 2_1163 1_1524 2_0115 ABG377861_1517902_0063 1_113893Bmag0225941_0583 2_06591031_0747 1_1021106ABG4991152_01301161_01841172_1083 2_13811182_1495 1_06621242_14931252_00091272_1081 2_0023 1_1392 2_11611282_12771321_0584136scsnp00940 1_06801371_13301432_09441442_1405 1_09181461_0821 1_0754 1_13381522_04131532_0369 1_1196 2_0662 1_1141 ABG0042_0343 1_0505158

2_0612 1_08421592_0527162scind022811692_0085 1_1328170MWG8831752_13861801_12971822_15311862_0421189HVM62 2_1345 1_14361902_1008 1_0935 1_0702 2_1237196ABC805 2_06051981_0893 1_0629 1_06811991_00142001_15162022_01762042_15002061_0343 1_0215 ABC172 2_1387208

3H

2_11170MWG63472_027491_1485161_049017MWG077241_0574 2_0557 HVM40 1_0223261_0221271_1199281_1136292_1458311_1175 1_0626 2_1385 1_1060 1_05622_042232

2_0109 2_1418 2_0680 2_0777 CDO542scsnp0164833

CDO122 1_0031341_0810 hvknox3 2_1389381_0171 1_0347412_0240 1_0371 2_0011 2_0180 1_006342scssr20569 ABC303 1_0048441_0668 CDO795 1_0793472_0939 1_1112 2_1490492_1303 2_1071 2_0269 2_0289 1_09461_0942 1_1332 2_0426 1_0667 2_08311_0756 1_0411 2_0853 1_1042 1_11902_0496 1_0607 1_0261 1_0577 2_10731_0492 2_1137 1_0432 2_1029 scind037512_1442 DST-46 scind04312a 2_0525

53

2_1481 2_0898 2_0363 2_0412 2_06101_0527 1_1108 1_0262 scind01728 1_08812_1213 1_0568 1_0426 1_1232 1_0637Tef2 1_0088

55

2_0151 2_1400 1_0086 2_0361 2_1010592_1296 1_1427 2_0453 1_1207 2_1273622_0820 GBM1020632_0723 1_0052 2_0924 1_0053 1_1431Bmag0353 2_0062 1_0639 2_009864

1_1070651_060666scind10455701_1513 2_1504 1_0467711_0309 2_0143 2_0207 2_1499 1_1319762_1332791_0170 scssr14079 1_150082ABG472 1_1526832_0197 1_1213 1_0670 1_072384GBM1059 2_0765 1_1398852_0732921_1168931_0785 KFP22194Ebmac070196MWG652B 2_0762 1_1292 1_0666 2_0838971_1139 2_0454 1_0334 2_111199GBM10481031_11941051_1233 2_0237 1_07121091_1235 2_07011102_0883 1_1066 2_10351122_1130 Hsh 1_0697113HVM67 2_02241142_07601151_0610 2_1450 2_0272 2_0007116KFP241.1 2_0932117ABG601127

4H

2_055302_12219scssr0230613MWG618202_053322ABC483 2_001024ABG610252_1365262_1065 2_0873331_0974342_0761352_0576391_0580432_0980 2_0845 2_1391471_1318 ABG395481_086449scssr02503 1_1432562_1401 1_1399 1_0856 2_0481 1_14622_1040 1_0157 1_0481 1_0240 2_14471_1469 2_0700 1_0177 1_0995 1_11982_0101 2_0697 2_1011 2_0461 1_02522_1116 1_0318 1_0913 2_1308 2_08412_0179 2_0524 2_1350

57

1_1260 1_1204582_0396 2_0059611_0264 2_1536 2_0105632_0306 1_1240 1_1151 1_0899 1_15121_1363 2_0501 2_0981 1_023764

1_1523661_0725 NRG045A 1_108967scsnp0426068Ale 2_1036692_0392 1_0203 2_0278 1_1438 1_12491_064172

1_0548792_0297801_0953812_0818 2_0330851_0305871_0183 2_1128 1_0127 2_1133 ABC302901_0140 scind16991 2_0246 2_0096 1_01671_063393

2_0736 1_1515 2_006694scssr15334962_118698scsnp06144 1_11351011_06741061_05241091_09301101_05781112_0097 2_0449 srh 1_1134 1_05181_1473 1_0771112

1_13501202_04031212_01341231_00241252_1321 1_0844 1_08341262_0805 1_0507 1_08711281_0477130RSB001A 1_1341131scsnp00177 1_12451390SU-STS11451_12981461_15071482_14221502_0045 2_0405 ABG003B1511_14561522_0300 1_1535 1_02281531_14721562_0551 1_0755167Tef31751_00041762_0731 2_0830 1_0104 2_06761772_0100 1_1441 1_0080 2_1462 MWG8771_0333 2_0388178

2_01041822_01861841_14971851_0582 ABG496 1_0902 1_0741 1_09011_0536 1_0385 2_0317192

2_0876 2_05601931_0273 1_1464 2_0646 2_1018 1_13251_0336195

2_03342072_0829 E10757A scsnp021092112_08262121_1216 ABG3912151_0769 JS10B 2_05462161_07782211_06002221_0254 1_1010 ABC622 1_14502231_0736 DsT-33 2_01892252_13932311_03102321_1364 2_11082351_0732 1_0020 1_1413237MWG602A2382_1356 scssr03906 2_0402 1_0322239

5H

2_02320Bmac0316 2_088122_088231_116642_0262 2_0294 2_12045MWG602B 1_0064351_0023 2_1246372_0315401_0774 JS10A 2_0745471_0799 1_0994 GBM1021 1_0939541_0427551_0129 1_1455 1_0061 GBM1068642_0936662_0983 1_0575 1_0461 1_0199 1_09101_0811 1_0539 1_0817 2_0707 BG2992971_0355

68

2_0675 1_1205 1_1171692_144970HVM31711_1104 2_0651 1_1353 1_0497 rob2_0656 2_1158 1_0962 2_1473 2_03812_0720 2_1014 2_0601

73

1_0591 2_0567 2_0785 1_1487 1_03232_0799 2_0572 1_1312 scssr02093 1_06591_0848 1_0749 2_0142 2_1216 1_09541_1153 1_1442 2_0854 2_0946 Bmag00091_1140

74

1_0278 1_0345 2_0835 1_1181 2_02022_1114 1_051375

1_0803821_0964 ABG474 2_0266841_1321 1_0189852_1298 2_1225 2_1293 2_0904 1_03922_0148 1_126190

2_0714 ABG388 1_0504932_0468942_0620 2_0892 1_0596 2_0673992_0577 2_0848 scsnp21226 1_1349 2_07841002_0488 MWG820 2_06821031_03311091_0185 1_12461102_1404 2_03461111_11471131_04001192_1025 1_02021202_09961221_0595 GBM1008 2_0972 1_09781261_1494 1_01391272_0379129scind04312b132GBM1022 2_00361362_0355 Bmac00401442_04481452_0558 DsT-32B1472_14771551_0645 1_0071 2_1455 2_1467 1_00511_0107157

1_1187 DsT-281581_0748 DsT-741612_0907 2_08681621_1488 2_02111631_1111 MWG514 MWG798A 1_0390165

6H

2_1419 1_003601_1132 ABG704 1_068211_0894 2_0201 1_012121_117961_103171_103592_0227122_075513GBSS-I151_1052182_105019AW982580 2_0495231_0965 CDO475 MWG089301_0451311_0232 2_0192 ABG380 2_0993322_1292391_0979401_0368451_1339472_1491482_1326 2_0150491_0772 2_0031522_011356scsnp0046060ABC255 1_040361ABC165D 1_0721621_0050642_0750 1_0657 2_1270652_0060 1_1028 1_1242702_1234 1_1257 1_1045 scssr15864 2_14921_1202 1_110375

1_0983 2_1167 2_088576GBM1030781_0083 1_1519801_1352 2_0879 2_1494 2_0828 MWG8081_0773 2_0200 1_0370 1_0698 2_13351_1291 1_0073 1_0268 1_0055 2_03141_0581 2_1319 2_1302 2_0893 1_0115DAK642 1_1145 2_1178 1_0924 2_0911

81

1_1029 1_1461881_0442 2_1330 1_0531 1_1091 scsnp007031_1445 2_0349 1_0673 2_089691

2_0083 1_0143 MWG2031 2_1409 2_077192nud952_1530 2_0685 1_0249 2_1501 1_1437962_1201 lks2111ABC10241142_08081152_0103 2_06111161_10511181_0169 2_1464120Bmag0120 2_08241221_0362 DsT-30 2_0406123WG380B1241_0563130ABC310B 1_11841342_0217 Ris44 1_12431372_03541411_1279 2_1229 2_12091481_0861 ABC2531521_0797159ABG461A 1_09731632_1280 1_0550 2_07531662_0414 1_1440 DsT-69 2_0452 WG380A2_1363 2_1223 1_0843167

2_06401681_01301701_0896 2_0962 1_1309 1_1275171GBM10651741_0378 KFP255198ThA1200

7H

Page 49: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

Oregon Wolfe Barley Map187 classical + 555 pilot-OPA1 + 467 pilot-OPA2 + 722 DArT = 1931 loci

bPb-63180bPb-7112 BCD1434 2_027511_0460 1_131171_0410 bPb-0181 DsT-6681_063691_101111Act8A bPb-9608 bPb-1318 bPb-7137 bPb-7043122_122614RbgMD [105102] [220835] [222208]18[222789]19MWG837B bPb-788922scind00046 ABC165C26[223986]27Bmac039929GBM1007301_0186 bPb-2166 [105118] 2_071233[221879] BCD09835bPb-3217 bPb-4813 bPb-9418421_0760 [104834] [104921]47GBM1042 [221425] 1_081448bPb-5672 1_1498 1_1357 1_0238 bPb-9388bPb-1586 bPb-1535 bPb-8662 bPb-1231 bPb-139851

bPb-2175 bPb-0482 bPb-5164 [222328] 1_1266bPb-0357 [221108] 2_0514 bPb-160452

1_0294572_0047 2_1312 2_0757 [223055] 1_09381_1209 bPb-9360 1_0438 1_1336 2_10001_1301 1_1169 bPb-0579 1_1036 2_0427Bmag0211 2_0698 2_1357 [221321] 1_12872_0810 2_1408 2_0912

58

1_1107591_1217 2_1217 1_1359611_0198 bPb-3382 BG369940 bPb-1193 1_02931_1256 bPb-179162

1_0768 2_1333 1_144466bPb-1723 1_0201 2_1463 bPb-9005681_0617 1_1049692_1131 1_0302702_1431 bPb-8183 2_0642 [222086] bPb-1133721_1367 bPb-3389 1_0002 1_1062 bPb-533977ABC160782_0696 1_0463 1_016679bPb-8713 bPb-6117 1_1075 2_1176801_1267812_0121 1_0279 1_089083[224235]841_046690bPb-8477951_1463981_1144991_05351011_1439 2_0769 JS10C 2_0475 bPb-79631_1008102

1_12771032_0754 2_0169 bPb-2007107bPb-9180 2_1020 1_0111 1_1173 2_06132_0909 MWG706A [221687] bPb-1419 2_0187bPb-7429

112

[223108] 2_0780 KFP170 2_0921119bPb-9108 [221039] 2_06251202_0021123bPb-5014 bPb-2063 bPb-51981251_09051271_1409128[221902] 1_0854 1_03381302_11721351_03391361_1457137MWG2028 1_02071411_0808 bPb-5090 ABC261142bPb-9552 1_0693 KFP257B bPb-60651441_0903 bPb-65021462_03831481_12711492_1105 WMC1E81511_1509 MWG912 1_1320 1_00411532_0594155ABG387A bPb-5550 2_0840 scssr04163 bPb-2260bPb-3756 bPb-1487157

1H

[223556]01_0970 bPb-8959 bPb-3598 1_1407 bPb-3608bPb-5191 bPb-7892 bPb-0611 bPb-0516 bPb-0485[222367] [220862] bPb-7151

1

ABG058 2_14663scind02622 [222211] 1_071842_141551_1030 bPb-7969 bPb-5900 bPb-0471 [223937]bPb-0205 bPb-50337

bPb-5519 bPb-7024 bPb-7557 [223539] bPb-0615[221144] bPb-56889

1_0230 1_105912[223312]14bPb-6897 bPb-8399 bPb-4295 bPb-1708 bPb-4481[220974] ABG008 bPb-5444 [221686] bPb-6128bPb-9220 bPb-0715

15

bPb-2355 bPb-4715 2_1423 bPb-6879 bPb-2782bPb-2108 bPb-8374 [104949] [222296] [221518]bPb-6494 bPb-8986 [222714]

18

2_0724 bPb-8292201_104022bPb-4875 scssr10226 bPb-9682 2_1261 bPb-4261462_0593491_0891 bPb-3190 bPb-2501 bPb-7906 1_03072_0368 scssr0775950

2_1049511_1304 GBM1066521_0847531_1057 1_0342 2_1461 1_0919 2_03261_0837 1_0178 1_0525 Pox bPb-07221_0296 bPb-2230

55

1_0084 1_1493561_0715571_0486581_1340 1_0204 2_028160bPb-5057 bPb-9925 1_0498 1_1505631_1428 2_0674 bPb-0220 2_1005 [223952]1_0156 2_068865

2_0929 scssr03381 1_1302 1_0422 1_11311_0638 [221628] [222438]66

1_1400 1_152268bPb-6438 1_0927 2_0387 1_0147 2_07482_0235 bPb-3056 2_0917 bPb-6881 2_02342_0602

71

2_0643 1_0558 Hot1 2_0004722_0196 2_1337 2_0417 1_0474 1_00992_0458 1_1369 1_1155 [220925] 1_0947[222433] 1_1288 [220842] 1_1211 2_03782_1434 2_0032 2_0039 1_0883 2_12862_0878 2_0181 1_0154 1_0948 bPb-18362_0500 scssr02236 1_0485 Ebmac0684 1_0330

73

1_1384742_033575scssr00334761_0632 1_0436 1_0640771_1430 ABG356 2_1476 2_0442 2_03741_131678

bPb-0885 bPb-7256 1_1072 bPb-4837 2_1094GBM1023 bPb-6437 1_134780

bPb-4040 [222771] bPb-5440 1_1096 bPb-9754bPb-608883

2_0833 bPb-8100 bPb-7243 1_0407862_0528 2_0667871_140288[222968] scsnp03343941_1388951_0859 1_0786971_1100992_0340 vrs1 1_15331001_09361042_1351 1_0969105Bmag0125 2_0793106DsT-41109MWG5031142_1007 1_1307 bPb-7991 1_1285 bPb-3563bPb-1926 GBM1062 bPb-6194 bPb-4835115

KFP203116MWG882A 2_0923 1_03981191_0900 1_1466 2_1527120bPb-8737 1_1323 bPb-4577 bPb-3653 bPb-17721251_1094 bPb-6822128[223776] 1_0128 bPb-3870 1_1043 2_12381_0990 2_0955 1_0263 bPb-8274 bPb-24812_0064 ABG072 1_0989

135

1_1236 1_0707 2_0989 1_04291361_07391411_0780143bPb-8949 bPb-1266144[221435] 2_0141 2_1315 1_1365 bPb-6688bPb-0541 bPb-7992147

2_1459 1_1486 [222960] Ebmc0415 1_01092_1406 1_0446149

bPb-5412 cnx1 bPb-2971152[222464]1532_1336 1_0065 1_0472 2_1125 1_0383bPb-3858 2_0215156

1_1227158[221983] bPb-1505159Zeo11602_1181 2_0590165[223553]172bPb-7208 [221407] bPb-1566 bPb-4092 bPb-0303bPb-8530 bPb-5619 1_0315 1_0566 GBM1019bPb-1986 bPb-1184 1_1023 bPb-1815 bPb-5460

173

2_0943174Aglu5 Aglu41751_0937 MWG7201772_0957178bPb-1611 bPb-4232 bPb-1181 bPb-4601 bPb-7212181GBM1012 1_0329183bPb-3102 [223337] bPb-8698 1_0085 bPb-6296bPb-0305 bPb-9419 bPb-6048 1_1050 bPb-1085185

2_14141872_1496188MWG949A scssr08447190

2H

2_0090 2_0858 bPb-40220[221259]2bPb-2553 [224140] bPb-4895 [223116] 2_0797bPb-38153

1_143451_1453 2_0252 bPb-1264 2_09539bPb-3689112_1398 bPb-9945 2_1027 1_1005131_1310 2_0976 1_088614[221362]15[223396] [223877] [220882] [221142]16bPb-9745 2_0172 2_1410202_0742 bPb-7448 [221551] [221552] bPb-06541_056521

bPb-3865 bPb-3229 bPb-0663 bPb-0548 bPb-482423GBM1074 2_0982262_0552291_0691 1_0559 2_0794302_0455 [220909] 2_1402 MWG798B [221274]1_1237 2_096832

bPb-5289 bPb-9961 bPb-9903 [105024] bPb-2929332_0607 bPb-3030 2_1268 1_0026 Dst-271_152735

bPb-5892 1_0081 bPb-2993 [222320] bPb-7938bPb-2910 [222411] 1_0598 [222615] bPb-6275bPb-9129 1_0968 [221166] BCD706 [220886]bPb-2324

39

bPb-3332 bPb-0158431_1117 2_1259 2_1506492_118954bPb-4407 1_1069 2_0264551_110257bPb-0068 1_1387 [224015] [223843] bPb-677158alm 1_0233 1_1511592_0866601_0391 1_1265611_1337 Bmac0209 1_1283 1_1482 1_10992_0486 2_0408 1_1295 2_1062 2_14112_1472 2_1435 1_0784 2_0721 1_11252_0439 2_0591 1_1530 2_1147 2_05832_0801 bPb-8666 1_0925

63

1_0011 2_044464bPb-6491651_0653 1_0850 2_0108 2_1511 2_0325ABC325 1_1429 2_1064 1_067866

DsT-6770bPb-4757 2_0863 1_119171bPb-3805 1_1284 bPb-6923 1_1342 bPb-00402_0608 1_1152 2_150272

2_130575bPb-3317 bPb-0079 1_1314 1_0200 bPb-84101_139476

1_0452 2_0694791_0683 1_0609 2_0695 2_0521 2_0124801_1454 2_0362 2_1194 scssr25691 2_0597[223370] 1_0047 bPb-787283

2_0849 2_0115 1_1524 ABG377 2_1163851_1517882_0063 1_113892Bmag0225931_0583 2_06591021_0747 1_1021105ABG4991142_0130 bPb-6722115[221404] 1_01841162_1381 2_10831171_0662 2_14951232_14931242_0009 [223016]1261_1392 2_1161 2_1081 bPb-9336 2_00231272_12771311_05841351_0680 scsnp00940136bPb-3630 bPb-42091371_1330142bPb-9110 2_0944 bPb-1579 bPb-1961 bPb-7695143[223788] 1_0918 2_1405145bPb-4386 bPb-77851461_0821 1_0754 1_13381512_04131521_0505 2_0662 2_0343 1_1196 ABG0042_0369 1_1141156

2_0612 1_08421572_0527161scind022811681_1328 bPb-5796 2_0085 bPb-8506 bPb-8894169bPb-4830171MWG883173bPb-3109 bPb-0049 bPb-2737 2_1386 bPb-7833bPb-5129 bPb-8021179

bPb-3843 bPb-6228 bPb-3899 bPb-1253 bPb-2420bPb-4748 1_1297 bPb-4739181

bPb-07891832_1531 [222583] bPb-74131842_04211881_1436 bPb-0302 bPb-6631 bPb-1829 HVM622_1345 bPb-4456189

bPb-02001911_0702 2_1237 2_1008 1_09351942_0605 ABC8051961_0681 1_0893197bPb-4387 bPb-41691991_0629 bPb-1411200[222593] 1_0014 [222092]2011_1516 bPb-01642032_01762042_1500206[221662] bPb-5346 1_0343 bPb-8962 1_0215bPb-7724 [221492] bPb-3295 ABC172 bPb-52562_1387 bPb-7120 [223314] bPb-0136 [221355]

208

[221563]210

3H

2_11170bPb-9406 bPb-7534 bPb-69381MWG63472_027491_1485161_049017[222436] MWG077242_0557 1_0574 [104902] 1_0223 HVM40261_0221271_1199281_1136292_1458301_0626 1_1175 1_0562 1_1060 2_04222_1385 [222526]32

2_0109 2_1418 CDO542 2_0680 2_0777scsnp0164833

1_0031 CDO12234[222439]36hvknox3 1_0810 2_1389381_0347 1_0171401_0063 1_0371 2_0180 2_0240 bPb-64042_0011 [223373] bPb-661141

ABC303 scssr20569 1_0048441_0668 1_0793 CDO795471_1112 2_1490 2_0939491_0756 2_1029 [223751] 1_0261 2_08311_0492 1_0667 1_0946 [220846] scind037511_0577 2_0496 1_0411 DST-46 2_13032_0269 bPb-9504 2_1137 2_0525 2_10731_1190 1_0942 2_0289 2_1442 2_04262_0853 scind04312a [222294] 2_1071 1_06071_1042 1_1332 1_0432

52

1_0262 1_0568 2_1481 1_0881 2_03631_0527 [105086] 1_1232 2_1213 scind017281_0637 2_0412 2_0898 Tef2 2_06101_1108 1_0088 1_0426

55

2_0361 2_1400 2_0151 1_0086 2_101059bPb-2305 bPb-6872 bPb-9907 2_0453 bPb-69491_1427 2_1296 2_1273 1_120761

bPb-6259 bPb-7987 GBM1020 2_0820 bPb-4290621_1431 2_0924 1_0053 2_0723 [224001]2_0098 1_0052 Bmag0353 1_0639 2_006264

1_1070651_060666scind10455 [221608] bPb-4214701_0467 1_1513 2_150471bPb-0098 bPb-8013721_0309 2_0143 bPb-3556 2_0207 2_14991_131976

2_1332791_1500 scssr14079 1_017081bPb-3739 bPb-6096 bPb-7719 1_1526 ABG472bPb-999882

1_1213 1_0723 1_0670 2_0197832_0765 bPb-1999 bPb-0610 1_1398 GBM1059bPb-4750 bPb-001885

2_0732911_116893bPb-5245 KFP221 1_078594Ebmac0701962_0762 1_0666 2_0838 MWG652B 1_1292971_1139 1_0334 2_1111 2_045499bPb-1166 [223032] GBM1048 bPb-98591021_11941051_1233 bPb-2677 1_0712 2_02371091_1235 [220875] 2_0701 bPb-10401101_1066 2_0883 2_10351111_0697 [223550] 2_1130 Hsh112HVM67 2_02241132_07601152_0272 1_0610 bPb-3468 2_0007 2_1450[223334]116

KFP241.1 2_0932117bPb-5265 ABG601 bPb-9668126

4H

bPb-017002_0553 bPb-6485 bPb-605122_122112scssr0230615MWG618222_053324ABC483 2_001026ABG61027bPb-2872 2_136528bPb-0091 bPb-3830 2_0873 bPb-6568 2_1065bPb-2460 bPb-7407 bPb-0351 bPb-1807 bPb-807235

1_0974362_076138bPb-5166 bPb-2266402_0576 bPb-0536411_0580452_1391 2_0980 2_084549ABG395 1_1318501_0864 bPb-4273 bPb-2273 bPb-8589 bPb-413551scssr02503 1_1432581_1469 bPb-0949 [222988] 2_0179 2_08411_0177 1_0856 bPb-8929 bPb-5584 2_14472_1011 bPb-8675 1_0913 [223293] 2_11162_0101 1_0481 1_0157 1_0240 [221402]2_0697 1_1462 2_0481 1_0318 1_02522_0524 bPb-1046 2_1401 bPb-0786 2_1308bPb-4210 2_0700 2_0461 2_1040 1_09951_1198 1_1399 2_1350

59

1_1204 bPb-5727 bPb-2762 bPb-7881 bPb-55061_126060

2_0396 2_0059631_0264 bPb-7933 2_1536 2_0105652_0981 [221802] 1_1151 1_1512 bPb-05032_0306 1_1240 1_0899 1_0237 1_13632_0501

67

1_152368NRG045A 1_1089 1_072569bPb-7627 scsnp04260 [223210] bPb-6260702_1036 [224132] Ale bPb-8987711_1438 [223812] 2_0278 1_0641 [222177][223146] 1_1249 1_0203 2_039274

1_0548812_0297821_0953832_0818 [221220] bPb-9618 bPb-7763 bPb-2147bPb-4067 bPb-0686 2_033087

1_0305892_1133 1_0183 1_0127 2_1128 ABC302922_0096 scind16991 1_0167 1_0633 2_02461_014096

1_1515 2_0736 2_006697scssr15334982_1186100scsnp06144 1_11351031_06741081_05241111_09301121_05781131_0771 1_0518 srh 2_0097 2_04491_1134 1_1473114

bPb-2869 [105044] bPb-5532 bPb-90321151_13501222_04031232_01341241_00241271_0834 2_1321 1_08441282_0805 1_0507 1_0871130bPb-5596 1_0477 bPb-7395132RSB001A 1_1341133[221888] scsnp00177 [222196] 1_12451410SU-STS11471_12981481_15071502_1422 bPb-0710 bPb-61351512_0045 2_04051531_1456 [104960] ABG003B1542_0300 1_1535 1_02281551_1472158bPb-2960 bPb-4084 1_0755 2_0551 [221423][223247]169

bPb-6578 [222215] bPb-5845176Tef31771_00041782_0676 1_0104 2_0830 2_07311792_0100 1_0333 1_0080 2_1462 2_0388MWG877 1_1441180

[223182]182[105067] 2_01041832_01861861_14971871_0902 1_0741 1_0901 ABG496 1_03851_0536 2_0317 1_0582194

2_0560 2_08761951_1325 2_0646 1_1464 2_1018 1_03361_0273197

bPb-6195 bPb-9147 2_0334 [222383]2092_0829 E10757A scsnp021092122_0826213bPb-0618 ABG391 [221131] bPb-3138 1_12162171_0769 JS10B 2_0546218bPb-4595 bPb-6179 bPb-0835220[104899]2211_07782221_0600 [223803] [222776] [222906] bPb-0877bPb-4621 [223339]223

[224041] 1_1450 ABC622 1_0254 1_1010224DsT-33 2_0189 1_0736 bPb-9132 bPb-7421227bPb-98682302_1393232bPb-8754 1_03102331_1364 [222075] bPb-1217 bPb-6239 2_11082361_0020 1_1413 1_0732239bPb-7008 MWG602A bPb-3973 bPb-2419 [221531]2402_1356 bPb-4725 bPb-7292 bPb-9244 scssr03906bPb-7360 bPb-6124 1_0322 bPb-5766 2_0402241

[221535]242

5H

[223608] 2_0232 bPb-37800[223456] bPb-7030 2_0881 bPb-7323 [222141]Bmac03162

bPb-9768 2_088231_1166 2_0262 [221458] bPb-3532 bPb-90752_1204 [223070] bPb-6511 bPb-1001 2_0294bPb-8275

5

bPb-7193 bPb-38077[221961] [222053] bPb-4778 bPb-0572 bPb-6311[222316] bPb-5498 bPb-343320

[224154] bPb-849226MWG602B 1_006430bPb-7165 [223325] 2_1246 [223374] 1_002331bPb-5252 bPb-9749 bPb-6661 2_031535[222286] [221152]38[223808] [220998]40JS10A bPb-3921 2_0745 1_0774411_0799 1_0939 GBM1021 1_099448[223386] bPb-6002 1_0427 bPb-3927 [221169]bPb-059749

bPb-7324 [220812]50[221710]561_1455 1_0061 1_012958GBM1068 [223273]592_0936 bPb-5379 [220818]60bPb-6142 2_0983 2_0707 1_0811 bPb-14661_0817 1_0575 1_0910 1_0461 1_0355BG299297 1_0199 1_0539

61

2_0675 [223115] 1_1171 bPb-5270 bPb-25921_120563

2_144964HVM31652_1473 1_1104 1_1353 2_0720 2_06012_0651 2_0381 bPb-6567 bPb-9114 rob2_1014 bPb-3487 1_0497 1_0962 bPb-16662_1158 2_0656

67

[223845] [223825] 1_0659 2_0785 1_03232_0854 [224033] 2_0572 [223262] [224253][223002] [223658] [222814] [223207] [222899]1_1312 scssr02093 2_0946 1_1442 2_01421_1487 [221391] Bmag0009 [223671] [223028][222912] 1_0591 1_1140 2_0799 2_1216bPb-2593 1_0848 2_0567 1_0954 1_07491_1153

68

1_0345 [222484] 2_0202 2_0835 bPb-97021_1181 [221874] [221245] 1_0513 bPb-16571_0278 bPb-0019 bPb-5196 2_1114 [222527]

69

bPb-9051 bPb-9835 bPb-9082701_0803 bPb-4229 bPb-5517 bPb-9479762_0266 1_0964 bPb-3230 bPb-6721 bPb-4369ABG474 bPb-4409 bPb-834778

bPb-3068 bPb-1256 [224127] 1_0189 1_1321792_1225 2_1293 2_1298 2_0148 1_03921_1261 2_090484

2_0714 1_0504 ABG388872_0468881_0596 [223211] 2_0892 2_0673 2_0620932_0784 scsnp21226 2_0577 2_0848 1_1349942_0682 MWG820 2_0488971_03311031_0185 1_12461042_1404 2_03461051_1147107[222895]1121_04001131_0202 2_10251142_09961162_0972 1_0595 GBM1008 1_09781191_0139 [220914] 1_1494 [221783]1202_0379123bPb-6477 bPb-9063124scind04312b1262_0036 GBM1022129bPb-34701302_0355 Bmac0040137[221063] [221088] bPb-5587 2_0448138DsT-32B 2_05581402_14771491_0645 2_1467 1_0071 1_0107 2_1455bPb-7446 bPb-9413 1_0051151

DsT-28 1_1187152bPb-55151531_1488 2_0211 1_0748 DsT-74154bPb-2940 2_0868 bPb-6677 bPb-2863 2_0907bPb-3760155

1_0390 bPb-2304 1_1111 bPb-1029 bPb-0403159bPb-9817 [223186] bPb-2410 [222800] bPb-9890160bPb-0649 MWG798A bPb-4626 MWG514 bPb-1621bPb-7146161

[220848]162

6H

1_0036 2_141901_0682 ABG704 1_113211_0121 2_0201 1_08942bPb-3732 bPb-8084 bPb-8272 bPb-4457 bPb-7559bPb-0398 [223392] [223152] bPb-7349 bPb-08443

1_11795[221162] [221346] 1_1031 bPb-4445 [224158][222156]7

bPb-2595 1_10359[221786] [223056] bPb-581610bPb-9202 bPb-5897112_0227 [222301] bPb-4634122_075513GBSS-I151_1052 bPb-6453 bPb-3727172_105019bPb-4266222_0495 AW98258023[222485]25MWG089 CDO475 bPb-8660 1_0965301_045131bPb-8939 bPb-0678 2_0192 ABG380 bPb-5494bPb-9601 [221832] 1_0232 bPb-0324 2_0993bPb-2533 bPb-5852 bPb-1360 bPb-5074 bPb-6156

32

2_1292391_0979401_0368441_1339472_1491482_0150 2_1326491_0772 2_0031522_0113 bPb-867455scsnp00460601_0403 ABC255611_0721 [221740] ABC165D [220928]621_0050632_0750 1_0657 2_127064bPb-1952 bPb-3561 bPb-7952 bPb-4219681_1028 1_1242 2_006070bPb-1447 bPb-805173[221264] 1_1103 [221655] 1_1045 scssr15864bPb-1093 2_1234 1_1257 2_1492 [222063]1_1202 bPb-5458

74

2_0885 [104913] 2_1167 [222546] 1_098375GBM1030771_1519 1_0083801_0773 2_1319 2_0314 1_1352 1_0581DAK642 1_0370 bPb-5126 bPb-2097 1_0115bPb-6975 2_1494 bPb-1770 2_0879 2_1178bPb-3227 2_0911 bPb-8956 1_1145 bPb-21882_1335 2_0893 2_0828 bPb-7915 1_09241_1291 1_0055 1_0268 MWG808 2_02002_1302 bPb-8396 1_0073 bPb-5599 bPb-23791_0698

81

bPb-9825831_1029 1_1461872_0349 scsnp00703 1_0673 2_1330 1_04421_0531 2_0896 1_1445 1_109190

1_0143 2_0771 2_0083 MWG2031 2_140991[221574]92bPb-6233 nud bPb-8439942_1501 2_0685 2_1530 1_0249 1_143795bPb-6386 bPb-7399 bPb-5296 bPb-2561 bPb-9912bPb-1847106

2_1201 lks2109ABC10241132_0808114bPb-1079 2_0103 2_0611 [222171]115[220920] 1_10511161_0169 2_1464118bPb-4191119Bmag0120 2_0824120DsT-30 1_0362 2_0406121WG380B122bPb-0027 bPb-9908 1_0563 bPb-2433128bPb-4272 [105081]1291_1184 ABC310B132bPb-8498134bPb-4389 bPb-6186 2_0217 Ris44 1_12431352_03541391_1279 2_1229 2_1209146[221079]1471_0861 ABC253150bPb-7739 1_0797157ABG461A bPb-8644 bPb-1556 1_0973 bPb-0758bPb-4394 bPb-5923 bPb-0889 [222159] bPb-6167bPb-0917

161

2_1280 bPb-7983 bPb-0981 1_0550 bPb-1631[220949] 2_0753 bPb-3980164

2_0414 1_0843 1_1440 2_1363 DsT-69bPb-8833 WG380A [222528] 2_0452 2_1223165

2_06401661_0130 bPb-2121 bPb-3232 bPb-9948 [223928]bPb-5898168

2_0962 1_0896 1_1275 1_1309 [221299]169GBM1065172[223404]173bPb-0328 KFP255 bPb-9884 1_0378196ThA1198

7H

Page 50: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

Oregon Wolfe Barley Linkage Map - (2383 loci, Haldane cM)

bPb-631802_0275 BCD1434 bPb-711211_1311 1_04607DsT-66 1_0410 bPb-018181_063691_1011 3_093311bPb-9608 bPb-7137 Act8A bPb-1318 bPb-7043122_1226143_0919 [222208] 3_0817 3_0950 [220835]3_1533 3_0952 RbgMD [105102]18

[222789]19bPb-7889 MWG837B22scind00046 ABC165C 3_095526[223986]27Bmac039929GBM1007 3_087630bPb-2166 1_0186 [105118] 2_0712 3_024133[221879] BCD098353_127638bPb-3217 bPb-4813 bPb-9418421_0760 [104834] [104921]47GBM1042 [221425] 1_081448bPb-1586 bPb-1231 1_0238 bPb-5672 bPb-1398bPb-9388 bPb-1535 3_0336 1_1357 bPb-86621_1498

51

bPb-0482 2_0514 bPb-5164 [222328] 3_02681_1266 bPb-2175 [221108] bPb-1604 bPb-035752

1_0294572_0810 3_1191 2_0427 [221321] 1_04383_0436 1_1287 bPb-9360 1_1169 2_06983_0258 [223055] 1_0938 2_1312 1_12092_0757 3_1381 1_1336 2_1357 2_14083_1134 Bmag0211 3_0762 3_0562 2_10003_0498 bPb-0579 3_0462 2_0047 2_09121_1036 1_1301

58

3_0774 1_1107 3_0522591_1359 2_1217 1_121761bPb-1791 bPb-3382 3_0243 3_0741 1_0198bPb-1193 3_0710 BG369940 1_0293 1_12563_0348

62

3_0343642_1333 1_1444 1_076865bPb-1723 bPb-9005 1_0201 3_1532 2_1463681_1049 3_0744 1_0617 3_0304692_1131 1_0302703_1179 3_140171bPb-8183 2_0642 bPb-1133 [222086] 2_1431723_0295 1_1367 bPb-5339 bPb-3389 1_10621_000276

ABC160783_0505 1_0166 2_0696 3_0298 1_0463791_1075 3_0742 2_1176 bPb-6117 bPb-8713801_1267811_0279 2_0121 1_089083[224235]841_046690bPb-8477 3_0072941_1463983_0204 1_1144991_05351012_0475 JS10C 1_1008 1_1439 bPb-79632_0769 3_1192102

1_12771032_0754 bPb-2007 2_01691061_0111 1_1173 2_0187 MWG706A 2_09092_1020 [221687] bPb-9180 2_0613 bPb-1419bPb-7429

112

3_01911182_0780 KFP170 [223108] 2_0921119bPb-9108 2_0625 [221039]1202_00211223_1526 bPb-5198 bPb-5014 bPb-20631241_09051271_14091281_0338 3_0257 1_0854 [221902]1302_11721341_0339 3_11051351_1457136MWG2028 1_0207141ABC261 1_0808 3_1387 bPb-50901421_0693 bPb-9552 bPb-6065 KFP257B 3_06491431_0903 bPb-65021452_03831483_0803 1_12711492_1105 WMC1E81511_1509 MWG912 1_0041 1_13201532_05941543_1081 3_0048 bPb-1487 bPb-2260 2_0840ABG387A bPb-5550 scssr04163 bPb-3756156

1H

[223556]0bPb-3598 bPb-3608 bPb-7892 bPb-0611 [222367][220862] bPb-0485 bPb-5191 1_1407 bPb-0516bPb-8959 1_0970 3_1446 bPb-7151

1

2_1466 ABG05831_0718 scind02622 [222211]42_14155bPb-5900 bPb-7969 bPb-0471 bPb-0205 1_1030bPb-5033 [223937]7

bPb-7557 bPb-0615 [223539] [221144] bPb-7024bPb-5688 bPb-55199

1_1059 1_023012[223312]143_0775 bPb-6128 bPb-8399 3_0402 bPb-0715ABG008 [220974] bPb-1708 bPb-5444 bPb-44813_0155 bPb-9220 bPb-4295 bPb-6897 3_0631[221686]

15

[104949] bPb-6494 bPb-6879 bPb-8374 [222296]bPb-2355 bPb-2782 2_1423 [221518] bPb-2108bPb-4715 [222714] bPb-8986

18

2_0724 bPb-8292201_104022bPb-9682 bPb-4261 bPb-4875 scssr10226 2_1261462_059349scssr07759 1_0307 bPb-2501 1_0891 2_0368bPb-3190 bPb-790650

2_104951GBM1066 1_1304521_0847531_0919 1_1057 Pox 3_0420 2_03262_1461 3_0657 bPb-2230 bPb-0722 1_03421_0837 1_0296 1_0178 1_0525

55

1_1493 1_0084563_0379 3_0029 1_0715573_0491 1_0486581_1340 2_0281 1_0204601_0498 bPb-9925 1_1505 bPb-5057632_0688 1_0156 [223952] bPb-0220 2_06743_0691 3_0604 1_1428 2_100565

[221628] 1_1302 1_1131 scssr03381 [222438]1_0422 2_0929 1_063866

1_1400 3_1474 1_1522682_0748 2_0234 2_0917 bPb-6438 1_0147bPb-6881 1_0927 3_0195 3_0251 2_02352_0602 2_0387 bPb-3056

71

Hot1 2_0643 1_0558 3_0557 2_00043_0209 3_048772

2_0032 3_1189 2_0378 2_0181 bPb-18361_0154 1_0485 [220842] 2_0196 1_13691_1211 3_0772 3_0206 2_1286 [222433]1_0947 3_0634 1_0330 [220925] 3_08531_0883 1_0099 1_1288 2_0417 scssr022361_0948 3_0068 Ebmac0684 2_1337 2_14342_0500 2_0039 3_1218 1_1155 2_08781_0474 2_0458

73

1_1384742_0335753_0245 scssr00334761_0640 1_0436 1_0632772_1476 2_0374 2_0442 3_1252 ABG3561_1430 1_1316 3_125678

bPb-4837 1_1347 bPb-7256 2_1094 bPb-64373_1307 1_1072 GBM1023 bPb-088580

bPb-5440 [222771] bPb-4040 1_1096 bPb-6088bPb-975483

bPb-8100 3_0205 1_0407 2_0833 bPb-72433_102186

2_0528 2_0667871_1402883_1394893_017891scsnp03343 [222968]941_1388 3_1445951_0859 1_0786971_110099vrs1 3_0901 2_0340 3_0897 3_09001_1533100

1_09361032_1351 1_0969105Bmag0125 2_0793106DsT-41109MWG503113bPb-4835 2_1007 bPb-7991 bPb-6194 1_1307bPb-1926 GBM1062 1_1285 3_0216 bPb-3563115

KFP2031161_0398 3_0200 2_0923 MWG882A1191_0900 1_1466 2_15271203_0480 3_0095121bPb-3653 bPb-4577 3_0555 bPb-8737 1_1323bPb-1772 3_0049125

1_1094 bPb-6822128[223776] 1_0128 2_0064 bPb-8274 bPb-3870ABG072 1_0990 1_0263 1_0989 2_0955bPb-2481 1_1043 2_1238 3_1402

135

1_0429 2_0989 1_1236 1_0707 3_04591361_07391401_0780 3_1406143bPb-1266 bPb-89491443_0097 bPb-0541 bPb-6688 [221435] 2_01412_1315 1_1365 bPb-7992 3_0152147

2_1406 3_0041 2_1459 1_1486 3_11001_0109 Ebmc0415 1_0446 3_0695 3_0310[222960]

149

bPb-5412 cnx1 bPb-2971151[222464]1523_0678 2_1125 1_0472 1_0383 2_13361_0065 bPb-3858 2_0215156

1_1227158[221983] 3_0396 bPb-1505 3_0106159Zeo11603_02481632_0590 2_1181165[223553]171bPb-1184 bPb-4092 GBM1019 1_1023 bPb-72081_0566 [221407] bPb-1815 1_0315 bPb-0303bPb-8530 bPb-5619 bPb-1986 bPb-5460 bPb-1566

173

2_0943174Aglu5 3_0823 Aglu4175MWG720 3_1300 1_09371772_0957178bPb-4601 bPb-7212 bPb-1181 bPb-4232 bPb-16111801_0329 GBM10121821_1050 bPb-6048 bPb-1085 bPb-9419 bPb-3102[223337] bPb-0305 bPb-6296 1_0085 bPb-8698185

2_14141872_1496188MWG949A scssr08447 3_0102190

2H2_0090 2_0858 bPb-4022 3_14280[221259]2bPb-3815 bPb-4895 bPb-2553 [224140] [223116]2_07973

1_143452_0252 3_1409 bPb-1264 2_0953 1_14539bPb-368911bPb-9945 2_1027 2_1398 3_0297 1_1005131_1310 2_0976 3_0910 1_088614[221362] 3_091515[220882] [223877] [223396] [221142]163_011319bPb-9745 2_0172 2_141020[221552] bPb-7448 2_0742 bPb-0654 [221551]1_056521

bPb-3865 bPb-0548 bPb-0663 bPb-3229 bPb-482423GBM1074 2_0982263_0192272_0552292_0794 3_0920 1_0691 1_0559301_1237 [220909] MWG798B 2_0455 [221274]2_0968 3_0284 2_140232

bPb-2929 bPb-5289 bPb-9903 [105024] bPb-9961331_1527 2_1268 2_0607 3_0431 1_0026bPb-3030 Dst-27 3_057135

bPb-2324 bPb-9129 bPb-2993 bPb-5892 1_0081[222411] bPb-7938 [221166] 1_0968 [222615]bPb-2910 BCD706 1_0598 [222320] bPb-6275[220886]

38

3_1298 3_1159 3_095341bPb-0158 bPb-3332432_1506 2_1259 1_1117 3_0913493_0609 2_118954bPb-4407 3_0467 2_0264 1_1069553_0583 1_1102 3_0721563_0922 bPb-6771 1_1387 3_0923 [223843]3_1015 bPb-0068 [224015]57

1_1511 1_0233 alm 3_0680592_0866603_0262 1_1265 3_1008 1_0391611_1099 1_0784 2_1062 3_1502 2_07213_0039 2_0408 1_1283 2_1435 1_11253_0130 1_0925 2_0591 bPb-8666 1_12952_0583 2_1411 1_1337 2_0439 2_11472_0801 Bmac0209 2_0486 1_1530 1_14822_1472

63

2_0444 1_001164bPb-6491652_1511 ABC325 1_0653 1_0850 1_06781_1429 2_1064 2_0325 2_010866

DsT-67701_1191 bPb-4757 2_0863711_1152 2_0608 2_1502 1_1342 bPb-38051_1284 bPb-6923 bPb-004072

2_1305 3_1153751_1314 1_1394 bPb-0079 bPb-3317 1_0200bPb-841076

3_0754771_0452 2_0694 3_1529783_0743 2_0695 3_0399 1_0609 2_01242_0521 1_068379

3_1346801_0047 2_0362 bPb-7872 scssr25691 [223370]1_1454 2_0597 2_119483

ABG377 1_1524 2_0115 2_0849 2_1163851_1517882_0063 1_113892Bmag0225933_0663983_03251012_0659 1_05831021_0747 1_10211043_1367108ABG499 3_0090113bPb-6722 3_0640 2_01301141_0184 [221404]1152_1083 2_13811161_0662 2_1495 3_03421222_14931232_0009 [223016] 3_01191251_1392 bPb-9336 2_0023 2_1161 2_10811262_12771293_03751313_13291321_05841341_0680 3_0928 scsnp00940135bPb-4209 bPb-36301361_1330141bPb-1579 bPb-7695 2_0944 3_1269 bPb-1961bPb-9110142

1_0918 [223788] 2_1405144bPb-4386 3_0274 bPb-77851451_1338 3_1525 3_0924 1_0754 1_08213_0084149

3_1238 2_04131503_05071523_00811531_1196 2_0369 2_0343 2_0662 1_1141ABG004 1_0505155

2_0612 1_08421563_09731582_0527 3_0092159scind02281166bPb-5796 3_0860 bPb-8506 2_0085 1_1328bPb-8894167

bPb-4830 3_1496169MWG883 3_0137172bPb-7833 bPb-3109 bPb-5129 bPb-0049 2_1386bPb-2737 bPb-8021177

bPb-2420 bPb-3899 bPb-3843 bPb-6228 bPb-47481_1297 bPb-4739 bPb-1253179

3_1161 bPb-07891822_1531 bPb-7413 [222583]1832_0421186HVM62 3_0370 2_1345 bPb-1829 bPb-4456bPb-0302 1_1436 bPb-6631187

bPb-02001893_0271 2_1237 3_0767 3_0960 2_10081_0935 1_0702193

2_0605 ABC8051951_0893 bPb-1411 1_0681 1_0629196bPb-4387 bPb-41691973_1388198[222593] [222092] 1_0014199bPb-0164 1_15162013_0229 2_01762033_13412042_15002053_0055206bPb-5256 3_1296 bPb-5346 2_1387 [223314]1_0343 ABC172 [221355] 1_0215 bPb-71203_0135 bPb-8962 [221492] bPb-7724 bPb-3295bPb-0136 [221662]

207

[221563]208

3H

2_11170bPb-6938 3_1324 bPb-7534 bPb-94061MWG63473_1486 2_0274 3_014091_1485161_0490 3_1458173_054023MWG077 3_0150 [222436]242_0557 [104902] 1_0574 HVM40 1_0223261_0221 3_1291271_1199281_1136292_1458302_0422 1_1060 1_0626 1_1175 3_03941_0562 [222526] 2_138531

2_0109 2_0777 2_1418 2_0680 CDO54232scsnp01648333_0907 1_0031 CDO12234[222439]363_116437hvknox3 3_1439 1_0810 3_1524 2_1389383_0992 1_0347 1_017140bPb-6611 2_0180 1_0371 bPb-6404 2_0240[223373] 2_0011 1_006341

3_0187 scssr20569 ABC303 1_0048431_0668 1_0793 CDO795472_1490 1_1112 2_0939492_0269 1_0946 3_0525 1_1332 2_13032_0525 3_1382 DST-46 3_0331 3_10263_1360 2_0831 1_0432 2_1442 3_0450[223751] 2_0289 1_0261 scind04312a 1_06073_0488 1_0492 [220846] 1_0667 3_11562_1071 2_0426 1_1042 bPb-9504 1_04111_0942 1_0756 3_0866 2_1073 1_11902_0853 scind03751 1_0577 2_1137 [222294]2_0496 2_1029 3_0777

52

3_0427532_1213 3_0060 1_0088 1_0527 scind017283_0839 1_0262 2_0898 2_1481 Tef21_1108 2_0412 [105086] 1_1232 1_08813_0034 1_0426 3_1297 1_0568 2_06101_0637 2_0363

54

2_1400 2_0151 1_0086 2_1010 2_0361592_1273 bPb-6872 1_1207 3_0706 2_12961_1427 bPb-9907 bPb-6949 2_0453 bPb-230561

bPb-4290 2_0820 bPb-7987 GBM1020 bPb-625962Bmag0353 2_0924 1_0639 2_0062 1_00522_0723 [224001] 2_0098 1_0053 1_143163

1_1070 3_0455641_060665[221608] bPb-4214 scind10455701_0467 2_1504 1_1513713_0994 bPb-0098 bPb-8013723_1258 3_1362732_0143 1_0309 2_1499 2_0207 bPb-35561_131975

2_1332793_0787 scssr14079 1_1500 1_0170 3_022681bPb-9998 bPb-7719 ABG472 1_1526 bPb-6096bPb-373982

1_0723 1_1213 1_0670 2_019783bPb-4750 1_1398 bPb-0018 bPb-0610 bPb-1999GBM1059 2_076584

3_0138882_0732913_0142 3_0232 1_1168921_0785 bPb-5245 KFP22193Ebmac0701953_0114 3_0046 2_0838 1_1292 3_05843_0117 1_0666 3_0158 MWG652B 2_076296

2_0454 3_0990 1_1139 3_1139 1_03342_111199

bPb-1166 bPb-9859 [223032] GBM10481021_11941043_0385105bPb-2677 2_0237 1_1233 1_07121092_0701 bPb-1040 1_1235 [220875]1102_1035 1_1066 2_0883111[223550] Hsh 2_1130 3_0146 1_0697112HVM67 2_02241132_07601143_0888 2_0007 2_1450 3_0884 3_08922_0272 3_0808 bPb-3468 3_0476 3_08873_0886 3_0889 VRN-H2 1_0610 3_0873

115

[223334] KFP241.1 2_0932 3_0239 3_00201163_1422117bPb-5265 3_0824 3_0825 ABG601 bPb-9668125

4H3_0163 bPb-017002_0553 bPb-6051 bPb-648523_098443_0001 3_0979 3_0975 3_0977 3_10223_10235

2_122111scssr0230615MWG618212_0533243_109425ABC483 2_001026ABG610 3_071427bPb-2872 2_1365283_0167 2_0873 bPb-3830 bPb-0351 bPb-1807bPb-8072 bPb-0091 bPb-2460 bPb-6568 2_1065bPb-7407

35

1_0974363_0531 2_076137bPb-5166 bPb-2266392_0576 bPb-0536401_0580452_0980 2_1391 2_084548ABG395 1_131849bPb-4273 bPb-8589 1_0864 bPb-2273 bPb-413550scssr02503 3_0105 1_1432 3_1492571_0913 [222988] 1_0995 1_0252 3_03542_1350 bPb-5584 2_1308 3_0792 3_15201_0177 3_0728 1_1469 2_0461 2_07001_0318 1_0240 2_0481 bPb-0786 [223293]1_1198 2_0179 1_1462 3_0729 3_1257bPb-4210 1_1399 2_0101 2_1011 2_05241_0481 bPb-8929 bPb-8675 3_1317 1_08562_1116 3_0337 bPb-0949 2_1401 2_10403_0109 [221402] 2_0697 2_0841 3_0768bPb-1046 3_1423 2_1447 1_0157 3_0709

58

1_1204 1_1260 bPb-7881 bPb-2762 bPb-5727bPb-550659

3_0214612_0396 2_0059631_0264 3_1117 2_0105 2_1536 bPb-7933653_1340 1_1240 bPb-0503 1_1512 1_11512_0306 1_0237 [221802] 2_0501 1_13633_0111 3_1062 3_1064 2_0981 3_03123_0538 1_0899 3_1183 3_1451

66

1_1523671_0725 NRG045A 3_1035 3_1034 3_10321_108968

bPb-7627 bPb-6260 [223210] scsnp04260 3_0745692_1036 [224132] Ale bPb-8987703_0080 [223812] 1_0641 1_1249 2_0278[222177] 3_0966 2_0392 1_1438 1_0203[223146]

74

1_0548813_0719 2_0297821_095383bPb-9618 [221220] 2_0330 2_0818 3_0252bPb-4067 bPb-0686 bPb-7763 bPb-214786

1_0305882_1128 1_0183 2_1133 1_0127 ABC302921_0140 2_0246 1_0167 2_0096 scind169911_063395

2_0066 1_1515 2_073696scssr15334973_0314 2_118699scsnp06144 1_11351031_06741071_05241111_09301121_05781131_1134 1_0771 1_0518 2_0097 3_08342_0449 3_0533 srh 1_1473114

[105044] bPb-2869 bPb-5532 bPb-90321153_01821161_13501223_0098 2_04031232_01341241_00241263_0846 3_0854 3_0847 1_0844 HvCBF91_0834 3_0850 3_0852 2_1321127

2_0805 3_0705 3_0807 1_0871 3_12361_0507129

bPb-5596 bPb-7395 3_0056 3_0456 1_0477131RSB001A1323_0619 1_13411331_1245 [222196] scsnp00177 [221888]1410SU-STS11461_12981481_1507150bPb-0710 2_1422 bPb-61351512_0405 2_0045152ABG003B 1_1456 [104960]1533_0169 3_0611 2_0300 1_1535 1_02281541_14721571_0755 2_0551 bPb-4084 [221423] 3_1465[223247] bPb-2960168

bPb-6578 bPb-5845 [222215]175Tef31761_00041773_1182 3_1234 2_0731 2_0676 2_08301_0104178

2_0100 1_1441 3_0062 1_0333 1_0080MWG877 3_1206 3_1050 2_1462 2_0388179

3_0795 3_1221 [223182]1822_0104 3_0830 [105067]1832_01861851_14971861_0902 1_0741 3_0238 1_0901 1_05821_0385 1_0536 2_0317 ABG496 3_0759193

2_0560 2_08761942_0646 1_1325 2_1018 3_0642 1_03361_1464 1_0273 3_1375196

bPb-9147 bPb-6195 [222383] 2_0334208scsnp02109 2_0829 E10757A2123_0666 2_0826213bPb-0618 ABG391 bPb-3138 1_1216 [221131]2161_0769 3_0087 JS10B 2_0546217bPb-4595 bPb-0835 bPb-6179219[104899]2201_07782211_0600 bPb-0877 [223339] [223803] [222906]bPb-4621 [222776] [224041]223

1_1450 1_1010 3_1404 ABC622 1_0254224DsT-33 2_0189 bPb-9132 bPb-7421 3_07691_0736 3_0504 3_0494226

bPb-98682292_1393231bPb-8754 1_03102322_1108 bPb-6239 3_1292 1_1364 bPb-1217[222075]236

1_0732 1_1413 3_1481 1_0020238MWG602A bPb-3973 bPb-2419 bPb-7008 [221531]239bPb-6124 scssr03906 bPb-9244 3_1123 2_13563_0958 bPb-4725 2_0402 bPb-7292 1_0322bPb-5766 bPb-7360

240

[221535]241

5H

[223608] 3_0319 bPb-3780 2_02320[223456] 2_0881 [222141] bPb-7030 Bmac0316bPb-73232

bPb-9768 2_088231_11664[223070] bPb-6511 bPb-8275 bPb-9075 2_1204[221458] bPb-3532 2_0262 bPb-1001 2_02945

bPb-3807 bPb-71937[221961] bPb-3433 [222053] bPb-4778 [222316]bPb-6311 bPb-0572 bPb-5498 3_001020

3_084222bPb-8492 [224154]261_0064 MWG602B30bPb-7165 2_1246 [223374] 1_0023 [223325]31bPb-9749 2_0315 bPb-6661 bPb-525234[222286] [221152]38[220998] [223808]401_0774 2_0745 JS10A bPb-3921413_0697423_0673443_130845GBM1021 1_0799 1_0994 1_093948[223386] 1_0427 bPb-0597 bPb-3927 [221169]bPb-600249

bPb-7324 [220812]503_052154[221710]553_0361571_0129 1_0061 1_1455 3_0783 GBM106858[223273]59[220818] 2_0936 bPb-5379601_0539 1_0817 1_0199 BG299297 1_09101_0461 1_0811 2_0983 3_1092 1_0355bPb-1466 2_0707 1_0575 3_0281 3_12743_0317 bPb-6142

61

1_1205 1_1171 bPb-2592 [223115] bPb-52702_0675 3_078262

2_144963HVM31 3_0569642_0656 3_0120 bPb-1666 1_0497 2_03811_1104 bPb-9114 2_0720 1_1353 bPb-65672_1014 2_0651 3_0021 3_1007 2_0601bPb-3487 2_1158 1_0962 2_1473 rob3_0032 3_1004

66

[223002] 1_0591 2_0567 1_0323 [222814]1_1442 2_0854 3_0305 scssr02093 [223671]3_1509 2_0799 3_0765 [223207] [222899]3_0230 2_0785 1_0659 [223845] bPb-2593[224033] [223262] 1_1153 2_0142 1_11401_1312 3_0567 2_0946 [224253] [221391]1_1487 2_1216 [223825] 3_1249 1_07491_0848 3_0508 1_0954 3_1443 [222912]3_0837 3_1187 [223658] [223028] Bmag00092_0572

67

1_0345 bPb-1657 bPb-5196 bPb-0019 2_0202[222484] 1_1181 [221874] [222527] 2_0835bPb-9702 1_0513 3_1174 2_1114 [221245]1_0278

69

bPb-9082 bPb-9051 bPb-983570bPb-5517 bPb-4229 1_0803 bPb-947975bPb-6721 bPb-4409 bPb-4369 1_0964 bPb-83472_0266 bPb-3230 3_1003 ABG47477

bPb-3068781_0189 bPb-1256 [224127] 1_1321791_0392 2_1293 1_1261 2_1225 2_09042_0148 2_129883

2_0714 ABG388 1_0504862_0468871_0596 2_0673 2_0892 [223211] 3_12892_062092

1_1349 2_0848 2_0784 2_0577 scsnp212263_125093

2_0488 MWG820 3_1111 2_0682 3_0236963_0573973_0148993_12531001_03311021_1246 1_01851032_0346 2_14041043_0452 1_1147106[222895]1111_04001121_0202 2_10251133_12351143_0324 3_0932 3_1225 2_09961151_0595 1_0978 GBM1008 2_09721183_1049 1_0139 3_1044 3_1048 3_13533_1043 [220914] 3_1042 [221783] 1_14943_1115

119

2_0379122bPb-9063 bPb-6477123scind04312b125GBM1022 2_0036128bPb-3470129Bmac0040 2_0355136[221063] [221088] 2_0448 bPb-5587137DsT-32B 2_05581403_1198 2_14771483_00251491_0645 1_0107 2_1467 bPb-9413 2_14553_1277 1_0071 1_0051 3_1392 3_0355bPb-7446

150

3_1126 DsT-28 1_1187 3_0057151bPb-55151521_0748 DsT-74 2_0211 1_1488153bPb-2863 2_0868 bPb-2940 bPb-3760 2_0907bPb-6677154

3_1498 3_1469156MWG514 1_0390 bPb-7146 bPb-1621 bPb-23041_1111 MWG798A bPb-1029158

bPb-9890 [222800] [223186] bPb-2410 bPb-9817159bPb-0649 bPb-4626 bPb-0403160[220848]161

6H

2_1419 1_003603_0472 1_1132 ABG704 1_068212_0201 1_0894 1_01212bPb-3732 bPb-7349 [223392] bPb-8272 bPb-0398[223152] bPb-4457 bPb-8084 bPb-7559 bPb-08443

3_135041_11795[221162] 1_1031 [224158] [222156] 3_1173[221346] bPb-44457

bPb-2595 1_10359bPb-5816 [221786] [223056]10bPb-9202 bPb-5897112_0227 [222301] bPb-4634122_0755133_0902 GBSS-I15bPb-3727 bPb-6453 3_0723 1_1052172_105019bPb-4266222_0495 AW98258023[222485]25MWG089 1_0965 CDO475 bPb-8660 3_07803_0329 3_135130

1_0451 3_0702311_0232 bPb-0324 ABG380 bPb-6156 bPb-96012_0993 3_0242 2_0192 bPb-5074 3_0083bPb-5494 bPb-0678 bPb-2533 bPb-5852 bPb-1360[221832] bPb-8939

32

2_1292393_0065 1_0979401_0368441_1339472_1491482_0150 2_1326493_0752 3_0371 2_0031 1_077252bPb-8674 2_0113553_0290563_0181581_0403 ABC255 scsnp00460 3_088061[220928] ABC165D [221740] 1_0721621_0050632_0750 3_0031 3_0639 1_0657 2_12703_146364

bPb-4219 bPb-1952 bPb-7952 bPb-3561672_0060 1_1028 1_124270bPb-1447 bPb-8051731_1045 [221655] bPb-5458 1_1257 1_1103scssr15864 3_0997 3_1452 [221264] bPb-1093[222063] 1_1202 2_1492 2_1234

74

3_1120 2_0885 [222546] 2_1167 1_0983[104913]75

3_0344 GBM1030771_1519 1_0083 3_0581802_0200 1_0581 bPb-2188 3_1140 1_06982_1494 1_0268 3_0492 3_0389 bPb-69753_0475 2_0911 DAK642 bPb-8396 bPb-7915bPb-3227 3_0411 3_0621 2_1319 3_1215bPb-5599 bPb-2097 3_0004 2_0879 bPb-89561_0055 3_0445 3_0449 1_1352 MWG8081_0924 2_1335 bPb-2379 3_1345 3_05742_1178 3_0563 1_0370 1_1145 2_08932_0314 1_0115 bPb-5126 1_1291 1_00733_1227 2_0828 3_0794 3_0550 3_0760bPb-1770 1_0773 2_1302

81

bPb-9825833_0565 1_1461 1_1029871_1445 1_0442 3_0199 2_0349 2_13301_0673 1_0531 scsnp00703 1_1091 3_11372_0896

90

2_0771 MWG2031 1_0143 2_1409 2_008391[221574]92bPb-8439 nud bPb-6233943_0301 2_0685 2_1530 1_1437 3_09962_1501 1_024995

bPb-2561 bPb-6386 bPb-9912 bPb-7399 bPb-1847bPb-5296106

lks2 2_12011093_1188 ABC10241123_0335 2_08081142_0611 [222171] 3_0168 2_0103 bPb-1079115[220920] 3_1294 3_1440 1_10511161_0169 2_1464118bPb-4191119Bmag0120 2_08241201_0362 2_0406 DsT-30121WG380B1221_0563 bPb-0027 bPb-9908 bPb-2433128bPb-4272 [105081]1293_0164 ABC310B 1_1184132bPb-84981333_1232 bPb-6186 1_1243 2_0217 bPb-4389Ris44135

3_1535 3_1483 2_03541392_1209 1_1279 2_1229146[221079]1471_0861 ABC2531503_0831 1_0797 bPb-77391573_03801593_1241 ABG461A bPb-0917 bPb-6167 bPb-1556bPb-8644 bPb-0758 bPb-0889 1_0973 [222159]bPb-5923 bPb-4394 3_1282

161

3_1325 3_1491 bPb-1631 bPb-0981 bPb-7983bPb-3980 2_1280 2_0753 [220949] 1_0550164

1_1440 3_1166 1_0843 2_1363 bPb-88333_0593 WG380A DsT-69 2_1223 2_04142_0452 [222528]

165

2_0640166bPb-3232 bPb-9948 1_0130 [223928] bPb-2121bPb-5898168

[221299] 1_1309 1_1275 2_0962 1_08961693_14891703_0244 GBM1065172[223404]173bPb-0328 bPb-9884 3_1019 KFP255 1_0378195ThA1198

7H

Page 51: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

bPb-6318 FGX_OWB00503bPb-7112 BCD14342_0275

0.0

1_0460 FGX_OWB004911_13112.5

FGX_OWB000863.3DsT-66 bPb-01811_04103.7

1_06365.01_1011 3_09336.2bPb-9608 bPb-7137bPb-7043 bPb-1318Act8A

7.4

2_12269.9[220835] RbgMD3_1533 [222208]3_0952 [105102]3_0950 3_09193_0817

13.7

[222789]15.0bPb-7889 MWG837B17.53_0955 ABC165CFGX_OWB00106 FGX_OWB00146FGX_OWB00046 scind00046

21.3

FGX_OWB00258 [223986]22.5Bmac039925.03_0876 GBM100726.2[105118] 2_07123_0241 bPb-2166FGX_OWB00114 1_0186

30.0

BCD098 [221879]32.53_127633.8bPb-9418 bPb-4813bPb-321737.6

1_0760 [104834][104921] FGX_OWB0016742.7

GBM1042 [221425]1_081443.9

FGX_OWB0020845.3bPb-5672 1_1357bPb-1535 bPb-8662bPb-1398 1_02381_1498 bPb-1231bPb-9388 bPb-15863_0336

46.4

bPb-0357 bPb-51642_0514 bPb-04821_1266 bPb-2175bPb-1604 3_0268[222328] [221108]

47.6

1_029451.4FGX_OWB0020052.01_0438 1_0938[223055] 1_1336bPb-0579 2_10001_1169 1_1287bPb-9360 2_04271_1036 2_13572_0047 FGX_OWB002493_0562 1_13013_1191 2_0757Bmag0211 3_0436FGX_OWB00391 3_1134[221321] 3_04623_0258 2_13123_1381 2_09123_0762 1_12092_1408 2_08102_0698 3_0498

52.7

3_0774 1_1107FGX_OWB00389 3_052253.9

1_1217 1_13592_1217 FGX_OWB0037656.4

bPb-1193 3_02431_1256 FGX_OWB002821_0293 bPb-1791BG369940 3_0348bPb-3382 3_07101_0198 3_0741

57.6

FGX_OWB00315 3_034358.91_0768 2_1333FGX_OWB00023 1_144460.1

2_1463 bPb-17233_1532 bPb-90051_0201

62.6

1_1049 3_07441_0617 3_030463.8

1_0302 FGX_OWB002162_113165.1

3_1401 3_117966.3bPb-1133 [222086]2_1431 2_0642bPb-8183

67.5

bPb-3389 bPb-53393_0295 1_13671_0002 1_1062

72.7

ABC16073.91_0166 3_0298FGX_OWB00418 3_05051_0463 2_0696

75.1

bPb-8713 bPb-61172_1176 3_07421_1075

76.4

1_126777.62_0121 1_02791_089078.9

[224235]80.11_046686.6FGX_OWB00063 3_0072bPb-8477 FGX_OWB0047991.7

1_146395.53_0204 FGX_OWB001051_114496.8

FGX_OWB00372 FGX_OWB002761_053599.3

FGX_OWB0000999.81_1008 JS10C1_1439 FGX_OWB003942_0769 2_04753_1192 bPb-7963

100.5

1_1277 FGX_OWB00449FGX_OWB00515101.7

2_0754 2_0169FGX_OWB00278 FGX_OWB00285FGX_OWB00193 bPb-2007

104.2

bPb-7429 2_10202_0909 FGX_OWB003341_0111 2_0613[221687] 2_0187MWG706A FGX_OWB00178bPb-1419 bPb-91801_1173

110.7

3_0191117.22_0780 KFP170[223108] 2_0921118.5

FGX_OWB00113 FGX_OWB00485[221039] bPb-91082_0625

119.7

2_0021122.2bPb-2063 bPb-50143_1526 bPb-5198124.7

1_0905 FGX_OWB00359FGX_OWB00525127.2

1_1409128.41_0854 1_03383_0257 [221902]FGX_OWB00045

130.9

FGX_OWB00256132.22_1172134.73_1105 1_0339FGX_OWB00239135.9

1_1457137.1MWG2028 1_0207140.91_0808 3_1387bPb-5090 ABC261142.2

3_0649 bPb-6065KFP257B bPb-95521_0693

143.4

bPb-6502 1_0903145.92_0383148.41_1271 3_0803149.62_1105 WMC1E8FGX_OWB00125 FGX_OWB00426FGX_OWB00090

152.1

1_1509 1_13201_0041 MWG912154.6

2_0594155.9FGX_OWB00031157.13_1081 bPb-2260bPb-5550 2_0840ABG387A bPb-14873_0048 FGX_OWB00197bPb-3756 scssr04163

158.3

1H

[223556]0.0FGX_OWB00196 bPb-8959FGX_OWB00441 1_1407bPb-7151 bPb-0516[220862] FGX_OWB00098FGX_OWB00453 bPb-5191bPb-3598 FGX_OWB00455bPb-0611 bPb-36081_0970 3_1446[222367] bPb-7892bPb-0485

1.3

FGX_OWB004632.0FGX_OWB004612.52_1466 ABG0583.71_0718 [222211]scind026225.0

2_14156.2[223937] FGX_OWB00005bPb-0205 bPb-5033bPb-7969 bPb-0471FGX_OWB00496 1_1030FGX_OWB00147 bPb-5900

7.4

bPb-0615 bPb-5688[223539] bPb-7557bPb-5519 bPb-7024[221144]

9.9

1_1059 1_023012.4FGX_OWB00138 [223312]13.7[221686] 3_0155bPb-6897 [220974]bPb-4481 bPb-9220bPb-4295 FGX_OWB00263bPb-6128 3_06313_0775 bPb-0715bPb-1708 FGX_OWB00403bPb-8399 bPb-5444FGX_OWB00061 ABG0083_0402

14.9

bPb-4715 [222714]bPb-2108 [104949]bPb-2355 bPb-8986[221518] [222296]bPb-6879 2_1423bPb-6494 bPb-8374bPb-2782

16.1

2_0724 FGX_OWB00507FGX_OWB00012 bPb-829217.4

1_104019.9bPb-4875 bPb-9682bPb-4261 scssr102262_1261

42.6

2_059343.91_0307 scssr07759bPb-2501 bPb-79062_0368 1_0891bPb-3190

45.1

FGX_OWB00480 2_104946.31_1304 GBM106647.6FGX_OWB00289 FGX_OWB00412FGX_OWB00073 1_084748.8

bPb-2230 2_14613_0420 1_05251_0342 FGX_OWB004832_0326 FGX_OWB00436FGX_OWB00522 FGX_OWB00129Pox 1_08371_1057 1_0178bPb-0722 3_06571_0919 FGX_OWB003631_0296

51.3

1_0084 1_149352.53_0379 FGX_OWB00118FGX_OWB00324 1_0715FGX_OWB00255 3_0029

53.8

1_0486 3_049155.02_0281 1_02041_134056.3

1_0498 bPb-50571_1505 FGX_OWB00287bPb-9925 FGX_OWB00338

58.8

FGX_OWB0020159.43_0691 2_10053_0604 1_0156bPb-0220 [223952]2_0688 1_14282_0674

60.0

1_0422 1_1302[222438] 2_0929[221628] scssr033811_1131 1_0638

61.2

1_1522 3_14741_140063.7

2_0748 2_03872_0602 2_0234bPb-3056 2_02352_0917 FGX_OWB004193_0195 1_0147bPb-6438 1_0927FGX_OWB00505 bPb-68813_0251

66.2

2_0004 1_05583_0209 3_04873_0557 2_0643Hot1

67.4

FGX_OWB0018968.03_1218 [220842]3_0772 2_1286FGX_OWB00107 2_0378bPb-1836 2_0500FGX_OWB00220 [222433]1_1211 1_04742_0878 2_1337FGX_OWB00175 2_00393_0206 2_14342_0181 1_09471_0330 2_0458[220925] 3_08532_0417 2_00323_0634 1_1369FGX_OWB00439 3_11891_0485 1_01541_0099 1_08833_0068 2_01961_1288 FGX_OWB00346scssr02236 Ebmac06841_1155 FGX_OWB001211_0948

68.7

1_138469.92_0335 FGX_OWB00401FGX_OWB0028071.1

FGX_OWB0012271.7scssr00334 FGX_OWB001003_024572.4

1_0436 FGX_OWB00313FGX_OWB00365 FGX_OWB000721_0632 1_0640

73.6

ABG356 3_12563_1252 2_14762_0442 1_13161_1430 2_0374

74.8

bPb-4837 GBM1023bPb-6437 bPb-72563_1307 2_10941_1072 1_1347bPb-0885

76.1

bPb-9754 [222771]bPb-4040 1_1096bPb-6088 bPb-5440FGX_OWB00227

78.6

2_0833 1_0407bPb-7243 bPb-81003_0205 3_1021

82.4

2_0528 2_066783.61_1402 FGX_OWB0000384.8FGX_OWB00079 3_139486.13_017888.6scsnp03343 [222968]91.13_1445 1_138892.31_0859 FGX_OWB004311_078694.8

1_110097.33_0901 1_15333_0897 3_0900vrs1 FGX_OWB003372_0340

98.6

1_0936102.41_0969 2_1351103.62_0793 Bmag0125FGX_OWB00508 FGX_OWB00026104.8

FGX_OWB00007107.3DsT-41 FGX_OWB00273109.8MWG503112.3bPb-4835 bPb-1926FGX_OWB00074 1_1285bPb-6194 FGX_OWB000221_1307 2_1007bPb-7991 GBM10623_0216 bPb-3563

113.6

KFP203114.82_0923 FGX_OWB00526MWG882A 1_03983_0200

117.3

1_0900 2_15271_1466118.5

3_0095 3_0480119.8bPb-3653 bPb-87371_1323 bPb-4577bPb-1772 3_05553_0049

122.3

1_1094 bPb-6822FGX_OWB00464126.1

FGX_OWB00183 bPb-2481FGX_OWB00209 bPb-82742_0955 bPb-38703_1402 [223776]FGX_OWB00060 1_09901_0128 2_0064ABG072 2_1238FGX_OWB00299 1_09891_1043 FGX_OWB000471_0263

132.6

3_0459 1_0707FGX_OWB00055 1_04292_0989 1_1236

133.8

1_0739 FGX_OWB00173137.61_0780 3_1406140.1bPb-8949 bPb-1266141.3bPb-0541 2_13153_0152 [221435]bPb-6688 3_00971_1365 bPb-79922_0141

143.8

[222960] 3_0695Ebmc0415 1_04463_0310 2_1459FGX_OWB00123 1_1486FGX_OWB00238 3_1100FGX_OWB00181 1_01093_0041 FGX_OWB003432_1406

146.3

bPb-2971 FGX_OWB00011cnx1 bPb-5412147.6

[222464]148.81_0065 1_04723_0678 2_11252_0215 bPb-38581_0383 FGX_OWB001532_1336

151.3

FGX_OWB00198 1_1227153.83_0106 3_0396bPb-1505 [221983]155.0

Zeo1156.33_0248158.82_1181 2_0590160.0[223553]167.9bPb-5619 bPb-1184bPb-1815 FGX_OWB001521_1023 bPb-8530FGX_OWB00229 bPb-1566bPb-4092 bPb-1986bPb-5460 GBM1019bPb-0303 [221407]1_0315 1_0566bPb-7208

169.2

2_0943 FGX_OWB00377170.4FGX_OWB00322 FGX_OWB00312Aglu4 FGX_OWB00132Aglu5 FGX_OWB000993_0823

171.6

1_0937 MWG7203_1300174.1

2_0957175.4bPb-4601 bPb-7212bPb-1611 FGX_OWB00004bPb-4232 bPb-1181FGX_OWB00294

177.9

1_0329 GBM1012180.41_1050 FGX_OWB00207bPb-0305 bPb-9419bPb-6296 bPb-60481_0085 bPb-1085bPb-3102 [223337]bPb-8698

182.9

2_1414 FGX_OWB00308184.12_1496185.3FGX_OWB00293186.63_0102 FGX_OWB00408MWG949A FGX_OWB00471scssr08447

187.8

2H

3_1428 bPb-40222_0858 2_00900.0

FGX_OWB002261.2[221259]2.5bPb-2553 [223116]2_0797 [224140]bPb-3815 bPb-4895

3.7

1_14346.22_0252 3_1409FGX_OWB00223 1_1453FGX_OWB00162 FGX_OWB004002_0953 bPb-1264

10.0

bPb-368912.51_1005 3_0297bPb-9945 2_10272_1398

13.7

1_1310 3_09102_0976 1_088615.0

3_0915 FGX_OWB00469[221362]16.2

[221142] [223396][223877] [220882]17.4

FGX_OWB00261 FGX_OWB000753_011319.9

bPb-9745 2_14102_017221.2

bPb-7448 2_07421_0565 [221551]bPb-0654 [221552]

22.4

bPb-0663 bPb-3229bPb-0548 bPb-3865bPb-4824

24.9

GBM1074 2_098228.73_019229.92_055231.22_0794 1_05591_0691 3_092032.4

[221274] 1_1237MWG798B [220909]3_0284 2_04552_0968 2_1402

34.9

bPb-5289 FGX_OWB00182[105024] bPb-9961bPb-2929 bPb-9903

36.2

1_0026 2_0607Dst-27 3_05713_0431 1_1527bPb-3030 2_1268

38.7

bPb-6275 1_0968[220886] bPb-2324bPb-7938 bPb-9129BCD706 [222320]bPb-2993 FGX_OWB00517bPb-2910 [222615][221166] bPb-58921_0081 [222411]1_0598

42.5

3_1159 3_09533_129845.0

FGX_OWB00210 bPb-3332bPb-015847.5

FGX_OWB0022150.0FGX_OWB0014151.23_0913 2_1506FGX_OWB00248 1_11172_1259 FGX_OWB00393

52.4

3_0609 2_118954.9bPb-4407 FGX_OWB004452_0264 FGX_OWB00237FGX_OWB00380 1_10693_0467

56.2

3_0583 3_07211_110257.4

bPb-0068 bPb-67713_1015 [224015][223843] 3_0922FGX_OWB00127 1_13873_0923

58.6

3_0680 1_1511alm FGX_OWB002971_0233

59.9

2_086661.11_1265 3_02623_1008 FGX_OWB00030FGX_OWB00429 1_0391

62.3

2_0591 1_09252_0486 3_15023_0039 1_10993_0130 2_11472_1472 1_12952_0408 FGX_OWB004162_1435 2_08011_1125 bPb-86661_1337 1_1530FGX_OWB00056 FGX_OWB000682_0721 1_0784FGX_OWB00399 2_04392_1411 2_0583FGX_OWB00300 1_12831_1482 Bmac02092_1062

63.6

1_0011 2_044464.8bPb-649166.01_1429 1_06531_0850 2_0108FGX_OWB00130 2_03252_1064 2_15111_0678 ABC325

67.3

DsT-6771.11_1191 2_0863bPb-475772.3

bPb-3805 2_0608bPb-6923 1_1152FGX_OWB00527 1_13422_1502 1_1284bPb-0040

73.5

2_1305 3_115377.31_1394 bPb-0079FGX_OWB00082 1_0200FGX_OWB00267 bPb-8410bPb-3317 1_1314

78.6

FGX_OWB0047079.33_075479.83_1529 1_04522_069481.0

1_0683 3_03993_0743 2_06952_0521 2_01241_0609

82.3

3_134683.51_0047 scssr25691bPb-7872 FGX_OWB00161[223370] 2_03621_1454 FGX_OWB004232_0597 2_1194

86.0

2_0849 2_11632_0115 ABG3771_1524

87.2

FGX_OWB00110 1_151789.72_0063 1_113893.5Bmag022594.83_0663101.33_0325103.81_0583 2_0659105.01_1021 FGX_OWB000181_0747107.5

FGX_OWB00043 FGX_OWB00117FGX_OWB00015 3_1367111.3

ABG499 3_0090FGX_OWB00230115.1

bPb-6722 3_06402_0130116.4

[221404] 1_0184117.62_1083 FGX_OWB002042_1381118.8

3_0342 2_14951_0662121.3

FGX_OWB00292 2_1493122.63_0119 [223016]2_0009123.8

FGX_OWB00342 2_1161FGX_OWB00111 2_0023bPb-9336 1_13922_1081

125.0

2_1277128.83_0375130.13_1329131.31_0584133.83_0928 scsnp009401_0680135.0

bPb-4209 bPb-3630136.31_1330140.1bPb-9110 2_09443_1269 bPb-1579bPb-1961 bPb-7695

141.3

1_0918 [223788]2_1405143.8

3_0274 bPb-7785FGX_OWB00180 bPb-4386145.1

1_1338 1_08213_0084 1_07543_0924 3_1525

146.3

2_0413 3_1238147.53_0507148.83_0081150.01_1196 1_0505ABG004 2_06622_0369 2_03431_1141

152.5

1_0842 2_0612153.73_0973156.2FGX_OWB00510157.0FGX_OWB00502 FGX_OWB003452_0527 3_0092157.5

scind02281162.6bPb-8894 bPb-57962_0085 1_13283_0860 bPb-8506

163.8

FGX_OWB00250 3_1496FGX_OWB00101 bPb-4830166.3

FGX_OWB00272167.63_0137 MWG883FGX_OWB00033168.8

bPb-5129 2_1386bPb-2737 bPb-3109bPb-0049 bPb-8021FGX_OWB00008 bPb-7833

173.9

FGX_OWB00257 FGX_OWB00495FGX_OWB00163175.2

bPb-4739 bPb-38991_1297 bPb-6228FGX_OWB00126 FGX_OWB00040bPb-2420 FGX_OWB00097FGX_OWB00392 FGX_OWB00137bPb-1253 bPb-3843bPb-4748

176.4

bPb-0789 3_1161178.9bPb-7413 FGX_OWB00169FGX_OWB00081 [222583]2_1531

180.1

2_0421182.6FGX_OWB00139 FGX_OWB002512_1345 bPb-18291_1436 bPb-4456FGX_OWB00493 3_0370HVM62 bPb-6631bPb-0302

183.9

bPb-0200 FGX_OWB00281186.41_0935 FGX_OWB004622_1237 1_07023_0960 3_07672_1008 FGX_OWB005183_0271 FGX_OWB00513

190.2

ABC805 2_0605192.71_0629193.9bPb-4169 bPb-4387195.13_1388 FGX_OWB00205bPb-1411 FGX_OWB003841_0681 1_0893

196.4

[222092] [222593]1_0014197.6

1_1516 FGX_OWB00270FGX_OWB00369 bPb-0164200.1

3_0229 2_0176201.33_1341202.62_1500203.83_0055205.0FGX_OWB00489205.72_1387 bPb-5256bPb-5346 FGX_OWB00228FGX_OWB00303 bPb-8962bPb-7724 ABC172bPb-3295 FGX_OWB004421_0343 FGX_OWB00468[221492] 3_0135FGX_OWB00058 bPb-0136[223314] bPb-7120[221662] 1_0215[221355] 3_1296

206.3

[221563]207.6

3H

2_11170.0bPb-7534 bPb-9406bPb-6938 3_13241.2

MWG6343.7FGX_OWB00432 2_0274FGX_OWB00001 3_01403_1486

6.2

1_148514.11_0490 3_145815.43_054021.9[222436] MWG0773_015023.1

1_0574 [104902]FGX_OWB00078 1_0223HVM40 FGX_OWB002192_0557

25.6

3_1291 1_022126.91_119928.11_113629.32_145830.6[222526] 1_10602_0422 1_11752_1385 1_06263_0394 1_0562

31.8

2_1418 FGX_OWB002322_0777 CDO5422_0109 2_0680

33.0

scsnp0164833.43_0907 CDO1221_003134.3

[222439] FGX_OWB0029135.53_116436.7hvknox3 3_1439FGX_OWB00053 1_0810FGX_OWB00284 3_15242_1389

38.0

1_0171 1_0347FGX_OWB00119 3_099240.5

[223373] bPb-64042_0240 2_00111_0371 2_01801_0063 bPb-6611

41.7

3_0187 scssr205691_0048 ABC30344.2

1_0668 1_0793CDO79548.0

1_1112 2_14902_093950.5

1_0756 [223751]3_0450 2_08532_0496 3_13821_0492 2_05252_1071 [220846]3_0525 DST-462_0269 1_09421_0577 3_13601_0432 2_14422_0426 2_10731_1190 3_1026FGX_OWB00378 scind037512_1029 2_02891_1332 2_13031_0261 FGX_OWB000522_0831 3_03311_1042 bPb-95041_0946 1_0607[222294] FGX_OWB003303_0777 FGX_OWB003983_1156 3_08661_0411 1_0667scind04312a 2_11373_0488 FGX_OWB00279

53.0

3_042754.21_0088 2_14811_1232 3_00341_0637 Tef21_0881 3_00602_0412 1_0568FGX_OWB00307 scind01728FGX_OWB00358 1_02623_0839 2_06103_1297 2_12131_0426 [105086]1_1108 2_03631_0527 2_0898

55.5

FGX_OWB0013456.0FGX_OWB0022256.72_1010 2_14001_0086 2_03612_0151

59.2

2_0453 3_07061_1427 bPb-99071_1207 FGX_OWB002252_1296 bPb-6872bPb-2305 2_1273bPb-6949

61.7

GBM1020 2_0820bPb-4290 bPb-6259bPb-7987

62.9

2_0924 1_00522_0062 Bmag03531_1431 1_06392_0098 [224001]2_0723 1_0053

64.2

1_1070 3_045565.41_060666.6bPb-4214 scind10455[221608]71.8

2_1504 1_04671_151373.0

3_0994 bPb-8013bPb-009874.2

3_1258 3_136275.52_1499 2_01432_0207 FGX_OWB003411_1319 1_0309bPb-3556

76.7

FGX_OWB0015577.92_133280.41_1500 FGX_OWB000623_0787 1_0170scssr14079 3_0226

82.9

bPb-3739 1_1526bPb-7719 bPb-6096ABG472 bPb-9998

84.2

1_1213 1_07231_0670 FGX_OWB004062_0197

85.4

bPb-4750 GBM1059bPb-1999 2_0765bPb-0610 bPb-00181_1398

86.6

3_013889.12_073292.91_1168 3_02323_014294.2

1_0785 bPb-5245KFP22195.4

Ebmac070197.93_0584 1_1292FGX_OWB00050 2_07623_0046 3_0117MWG652B 2_08383_0158 1_0666FGX_OWB00160 FGX_OWB002033_0114 FGX_OWB00413FGX_OWB00029

99.1

1_0334 FGX_OWB00482FGX_OWB00382 FGX_OWB001091_1139 FGX_OWB00224FGX_OWB00321 3_0990FGX_OWB00246 2_04542_1111 3_1139

101.6

GBM1048 bPb-9859FGX_OWB00323 [223032]bPb-1166

104.1

1_1194 FGX_OWB00440FGX_OWB00112106.6

3_0385107.9bPb-2677 1_07121_1233 2_0237110.4

[220875] 2_07011_1235 bPb-1040111.6

2_1035 1_10662_0883112.9

3_0146 [223550]2_1130 1_0697Hsh

114.1

HVM67 2_0224115.32_0760116.63_0873 3_04763_0888 2_00073_0889 2_02723_0886 2_14501_0610 3_08843_0808 bPb-34683_0892 3_0887VRN-H2

117.8

[223334]118.22_0932 3_00203_0239 KFP241.1119.0

3_1422120.3ABG601 bPb-5265bPb-9668 3_08243_0825

126.8

4H

bPb-0170 3_01630.0bPb-6051 FGX_OWB00335bPb-6485 2_05531.2

3_09843.73_1023 3_09793_1022 3_00013_0977 3_0975

5.0

2_122111.5scssr0230614.0MWG61821.92_053324.43_109425.62_0010 ABC48326.9ABG610 3_071428.1bPb-2872 2_136529.4bPb-2460 bPb-0091bPb-6568 bPb-1807bPb-7407 bPb-3830bPb-0351 2_1065bPb-8072 2_08733_0167 FGX_OWB00025

35.9

1_097437.13_0531 2_076138.3FGX_OWB0031839.6bPb-2266 bPb-516640.8bPb-0536 2_057642.01_058045.82_0980 2_08452_139148.3

ABG395 1_131849.6FGX_OWB0029850.1bPb-2273 bPb-8589bPb-4135 bPb-42731_0864

50.8

1_1432 FGX_OWB000513_1492 3_0105scssr02503

58.7

2_1308 [222988]1_0481 FGX_OWB003813_0728 1_01771_0252 3_01092_1401 bPb-86752_0697 2_14471_0318 3_1317bPb-1046 1_01571_1469 2_10113_0354 3_1520FGX_OWB00430 bPb-89292_0481 3_07292_0461 3_12572_0524 2_10402_1116 FGX_OWB002413_0709 [221402]2_1350 1_0240[223293] bPb-09493_1423 bPb-5584FGX_OWB00288 bPb-0786bPb-4210 2_07001_0995 1_08563_0768 3_07921_1462 1_13991_1198 FGX_OWB00179FGX_OWB00301 3_03372_0841 2_0101FGX_OWB00006 2_0179FGX_OWB00041 FGX_OWB001481_0913

59.9

FGX_OWB0017760.7bPb-5506 FGX_OWB001681_1260 bPb-7881bPb-2762 1_1204bPb-5727

61.2

3_021462.42_0396 2_0059FGX_OWB00166 FGX_OWB0024363.6

2_0105 3_1117FGX_OWB00214 bPb-7933FGX_OWB00151 2_15361_0264

64.9

3_1062 3_03121_1240 2_03061_1512 3_10643_1340 3_0111[221802] 1_13633_0538 1_02372_0981 1_11513_1183 FGX_OWB001762_0501 3_1451bPb-0503 1_0899

66.1

1_152367.33_1032 1_07253_1034 NRG045A1_1089 3_1035

68.6

bPb-6260 FGX_OWB00039FGX_OWB00038 [223210]3_0745 scsnp04260bPb-7627

69.8

bPb-8987 Ale[224132] 2_103671.0

2_0278 FGX_OWB00159[222177] 3_00801_0203 1_14381_0641 [223812]2_0392 [223146]3_0966 1_1249

74.8

1_054881.33_0719 FGX_OWB002592_029782.6

1_095383.82_0818 bPb-7763bPb-9618 bPb-06863_0252 2_0330bPb-4067 [221220]bPb-2147

87.6

1_030590.12_1133 FGX_OWB004591_0127 2_1128ABC302 1_0183FGX_OWB00084

93.9

2_0246 FGX_OWB000852_0096 1_01671_0633 FGX_OWB005041_0140 scind16991FGX_OWB00002

96.4

1_1515 2_00662_073697.6

scssr1533498.92_1186 3_0314101.41_1135 FGX_OWB00351FGX_OWB00057 scsnp06144105.2

1_0674110.31_0524114.11_0930115.3FGX_OWB00190 1_0578116.61_1473 1_05181_0771 2_0449FGX_OWB00266 srhFGX_OWB00184 2_00973_0834 1_11343_0533

117.8

[105044] bPb-5532bPb-9032 bPb-2869119.0

3_0182120.3FGX_OWB00435124.61_1350126.63_0098 2_0403127.92_0134129.11_0024130.33_0846 FGX_OWB004883_0854 3_08501_0844 3_08472_1321 1_0834HvCBF9 3_0852

131.6

3_1236 3_07052_0805 1_0507FGX_OWB00013 1_08713_0807

134.1

FGX_OWB00414 bPb-73953_0456 3_00561_0477 bPb-5596FGX_OWB00326

136.6

RSB001A137.13_0619 1_1341FGX_OWB00017137.8

[221888] scsnp00177[222196] 1_1245FGX_OWB00164

145.7

FGX_OWB00268 0SU-STS1148.21_1298149.41_1507151.92_1422 FGX_OWB00142bPb-0710 bPb-6135FGX_OWB00390 FGX_OWB00379FGX_OWB00188 FGX_OWB00498

153.2

2_0405 2_0045154.41_1456 [104960]155.6ABG003B156.41_1535 3_06112_0300 3_01691_0228

156.9

1_1472 FGX_OWB00415160.7FGX_OWB00253 1_0755bPb-4084 [221423]2_0551 bPb-2960FGX_OWB00102 [223247]3_1465

170.0

FGX_OWB00158175.2bPb-6578 [222215]bPb-5845176.4

Tef3177.61_0004178.93_1182 1_01043_1234 2_07312_0830 FGX_OWB00024FGX_OWB00262 2_0676

180.1

2_0100 MWG8771_1441 3_00622_0388 3_1206FGX_OWB00192 1_00803_1050 FGX_OWB003192_1462 1_0333

181.3

3_1221 3_0795[223182]183.8

[105067] 3_08302_0104185.1

2_0186186.31_1497187.51_0582 2_0317FGX_OWB00331 3_0759FGX_OWB00092 FGX_OWB001851_0536 ABG4961_0741 3_0238FGX_OWB00014 1_09021_0901 1_0385

191.3

FGX_OWB00010192.1FGX_OWB00501 FGX_OWB000662_0560 2_0876192.6

FGX_OWB00521 2_1018FGX_OWB00512 1_02733_0642 3_13751_0336 1_14642_0646 FGX_OWB00356FGX_OWB00306 1_1325

195.1

FGX_OWB00187201.3[222383] 2_0334bPb-9147 bPb-6195202.8

E10757A scsnp021092_0829205.3

2_0826 3_0666206.5bPb-3138 ABG3911_1216 [221131]FGX_OWB00156 bPb-0618

209.0

FGX_OWB00524 3_00871_0769 JS10B2_0546

210.2

bPb-6179 bPb-4595bPb-0835212.8

[104899]214.01_0778215.21_0600 bPb-0877[223803] bPb-4621[222906] [223339][222776]

216.5

[224041]217.01_1450 3_1404ABC622 1_02541_1010

217.7

bPb-9132 3_04941_0736 2_01893_0769 3_0504DsT-33 bPb-7421FGX_OWB00035

220.2

bPb-9868224.02_1393226.5bPb-8754 FGX_OWB000361_0310227.7

3_1292 bPb-62391_1364 FGX_OWB002442_1108 bPb-1217[222075]

231.5

1_0732 FGX_OWB002401_1413 3_14811_0020

234.0

bPb-2419 bPb-7008FGX_OWB00340 FGX_OWB00206bPb-3973 MWG602A[221531]

235.3

3_0958 bPb-6124bPb-7360 bPb-5766bPb-7292 1_03222_0402 scssr03906bPb-4725 3_11232_1356 bPb-9244

236.5

[221535]237.8

5H

[223608] 2_02323_0319 bPb-37800.0

FGX_OWB00325 FGX_OWB00360FGX_OWB003861.2

FGX_OWB001991.9[222141] FGX_OWB00154[223456] Bmac0316bPb-7323 bPb-70302_0881

2.5

bPb-9768 2_08823.71_11664.3bPb-9075 [223070]bPb-3532 bPb-10012_0294 2_12042_0262 bPb-8275[221458] bPb-6511

4.9

FGX_OWB00202 bPb-3807bPb-71936.2

bPb-5498 bPb-63113_0010 bPb-3433[222316] bPb-0572[222053] FGX_OWB00269bPb-4778 FGX_OWB00486[221961]

20.1

3_084222.6FGX_OWB0039523.9[224154] bPb-849226.41_0064 MWG602B27.6[223325] [223374]2_1246 bPb-71651_0023

28.8

bPb-9749 bPb-6661bPb-5252 2_031532.6

[221152] [222286]36.4FGX_OWB0023337.7[223808] FGX_OWB00242[220998]38.9

bPb-3921 1_0774JS10A 2_074540.1

3_069741.43_067342.63_130843.81_0994 GBM1021FGX_OWB00120 1_07991_0939 FGX_OWB00076

46.3

bPb-6002 [223386][221169] bPb-3927bPb-0597 1_0427

47.6

FGX_OWB00089 bPb-7324[220812]48.8

3_052152.6[221710]53.93_036155.11_0061 1_01291_1455 3_078356.3

GBM106857.1[223273]57.52_0936 [220818]bPb-5379 FGX_OWB0011658.8

3_0317 3_1274FGX_OWB00077 1_04611_0575 1_09103_0281 1_0539BG299297 FGX_OWB002602_0983 1_08172_0707 1_01991_0811 1_03553_1092 FGX_OWB00336FGX_OWB00327 bPb-1466FGX_OWB00186 bPb-6142

60.0

2_0675 [223115]bPb-5270 1_11713_0782 bPb-25921_1205

61.3

2_144962.53_0569 FGX_OWB00476HVM3163.7

2_0651 1_09622_0381 2_11583_0120 bPb-91142_0601 1_1353rob 3_10073_0021 3_0032bPb-6567 bPb-16662_0720 bPb-34873_1004 2_06561_0497 1_11042_1014 2_1473

65.0

1_0954 1_0749[223262] [223845]3_0765 [223825]1_1140 1_14421_0848 [223671][223028] 2_07991_1487 3_15091_0591 [221391]3_1443 [222899][223002] 3_08372_0785 2_08543_1249 1_1312[223658] [222912]scssr02093 FGX_OWB002123_1187 1_06593_0567 bPb-25933_0230 [224253]2_1216 2_0946[222814] 2_01421_0323 Bmag00092_0567 1_1153FGX_OWB00494 3_0305[223207] 3_05082_0572 FGX_OWB00290[224033]

66.2

bPb-1657 1_02781_0513 3_11742_0202 bPb-97021_0345 FGX_OWB002132_1114 FGX_OWB00059bPb-5196 bPb-00192_0835 [221874]1_1181 [222527][222484] [221245]

67.4

bPb-9835 bPb-9082bPb-905168.7

bPb-4229 1_0803bPb-9479 bPb-551771.2

FGX_OWB00344 bPb-4369ABG474 1_0964bPb-6721 bPb-8347bPb-3230 FGX_OWB001312_0266 3_1003bPb-4409

72.4

bPb-306873.3bPb-1256 1_01891_1321 FGX_OWB00096[224127]

73.6

2_1293 2_12982_0904 2_12251_1261 1_03922_0148

78.8

2_0714 FGX_OWB00218FGX_OWB00020 ABG3881_0504

81.3

2_046882.51_0596 [223211]3_1289 2_06202_0892 2_0673

87.6

FGX_OWB0034988.22_0784 1_1349scsnp21226 3_12502_0577 2_0848

88.9

3_0236 FGX_OWB00028FGX_OWB00171 2_06823_1111 FGX_OWB00088MWG820 2_0488

91.4

3_0573 FGX_OWB0007192.6FGX_OWB00115 3_014893.83_125395.1FGX_OWB00407 FGX_OWB0042197.61_0331100.11_0185 FGX_OWB005141_1246101.3

2_0346 2_1404102.53_0452 1_1147105.0[222895]110.2FGX_OWB00254110.91_0400111.42_1025 1_0202112.63_1235113.93_0932 3_03242_0996 3_1225115.1

2_0972 GBM10081_0595 1_0978117.6

3_1048 FGX_OWB002751_1494 3_10493_1043 [220914][221783] 3_13533_1115 3_10423_1044 1_0139

118.8

2_0379121.3bPb-6477 FGX_OWB00217FGX_OWB00295 FGX_OWB00172bPb-9063

122.6

scind04312b123.82_0036 GBM1022127.6bPb-3470128.8Bmac0040 2_0355136.7[221063] [221088]bPb-5587 2_0448138.0

2_0558 DsT-32B139.23_1198 2_1477148.63_0025 FGX_OWB00069149.8bPb-7446 2_14553_0355 bPb-94131_0071 1_00511_0107 3_13923_1277 1_06452_1467

151.0

3_0057 3_1126FGX_OWB00428 DsT-281_1187

152.3

bPb-5515153.51_1488 FGX_OWB002962_0211 FGX_OWB00080DsT-74 FGX_OWB002861_0748

154.7

bPb-3760 bPb-2940bPb-6677 2_0907bPb-2863 2_0868

156.0

3_1498 FGX_OWB00424FGX_OWB00446 3_1469158.5

bPb-2304 FGX_OWB00174bPb-0649 1_0390159.7

bPb-2410 [222800]FGX_OWB00329 [223186]bPb-9890 bPb-9817

160.9

bPb-0403 bPb-7146bPb-1621 MWG514FGX_OWB00236 bPb-4626bPb-1029 MWG798A1_1111

162.2

[220848]163.4

6H

2_1419 1_00360.0FGX_OWB005090.5FGX_OWB003040.91_0682 3_04721_1132 ABG704FGX_OWB00305

1.2

2_0201 1_01211_08942.5

bPb-37323.1bPb-0844 bPb-8272bPb-0398 bPb-7559bPb-4457 [223152]bPb-7349 bPb-8084[223392]

3.7

FGX_OWB004814.43_13504.9FGX_OWB003175.31_1179 FGX_OWB000956.1FGX_OWB00302 [222156][224158] 1_1031FGX_OWB00032 FGX_OWB00523[221162] [221346]3_1173 bPb-4445

7.4

FGX_OWB000708.01_1035 FGX_OWB00211bPb-25958.6

[223056] [221786]bPb-5816 FGX_OWB002159.8

bPb-9202 FGX_OWB00452bPb-589711.1

2_0227 [222301]bPb-463412.3

2_075513.53_0902 GBSS-I16.0FGX_OWB0030917.43_0723 1_1052bPb-6453 bPb-372718.5

2_1050 FGX_OWB0048719.7bPb-4266 FGX_OWB0016523.5AW982580 FGX_OWB003752_049524.8

FGX_OWB00529 [222485]27.3FGX_OWB0024728.53_0329 bPb-86601_0965 3_0780MWG089 CDO4753_1351

32.3

3_0702 FGX_OWB001281_045133.6

FGX_OWB00170 bPb-0324bPb-1360 bPb-5494bPb-2533 bPb-8939bPb-5074 1_0232[221832] 2_0192bPb-9601 3_00833_0242 FGX_OWB00265bPb-5852 FGX_OWB003742_0993 ABG380bPb-0678 bPb-6156

34.8

2_129242.73_0065 1_097944.0FGX_OWB0050047.81_036849.0FGX_OWB00311 FGX_OWB000931_133951.5

2_149152.82_1326 2_015054.0FGX_OWB0024555.2FGX_OWB0006556.33_0752 3_03711_0772 2_003157.7

bPb-8674 2_011361.53_0290 FGX_OWB0008762.73_018164.0ABC255 3_0880scsnp00460 1_040367.8

FGX_OWB00016 [220928][221740] ABC165DFGX_OWB00448 1_0721

69.0

1_005070.2FGX_OWB00195 3_0639FGX_OWB00083 3_1463FGX_OWB00310 1_06573_0031 2_07502_1270

71.5

bPb-1952 bPb-4219bPb-3561 bPb-795275.3

FGX_OWB00034 FGX_OWB001911_1028 FGX_OWB000372_0060 1_1242

77.8

FGX_OWB0010879.0bPb-1447 bPb-805180.23_1452 FGX_OWB002642_1234 scssr158641_1045 bPb-1093FGX_OWB00352 1_1257FGX_OWB00314 FGX_OWB00149[221655] 2_14923_0997 FGX_OWB00157FGX_OWB00231 1_11031_1202 bPb-5458[221264] [222063]

81.5

1_0983 [222546]2_0885 2_11673_1120 [104913]

82.7

GBM1030 3_034485.21_1519 3_05811_008387.7

bPb-6975 3_05631_0268 3_06211_1145 2_08933_0475 FGX_OWB00042FGX_OWB00135 bPb-51263_0411 2_1178bPb-8956 3_07602_1494 2_0200bPb-2379 1_05813_0445 3_13453_0574 bPb-21883_0389 3_12273_0794 MWG8082_0879 DAK642FGX_OWB00271 FGX_OWB001032_0828 bPb-17701_0055 bPb-55991_0115 bPb-83962_1319 bPb-20971_0698 1_07733_0492 2_03142_1335 3_12151_1352 bPb-79153_0550 3_00041_0924 3_1140bPb-3227 2_13021_1291 1_0073FGX_OWB00150 1_03702_0911 3_0449

88.9

bPb-982591.43_0565 FGX_OWB002521_1029 FGX_OWB003971_1461

95.2

1_1091 2_03493_0199 1_05311_0442 1_06733_1137 FGX_OWB00274scsnp00703 2_08961_1445 2_1330

97.7

2_0771 MWG20312_1409 1_01432_0083

99.0

[221574]100.2bPb-8439 nudbPb-6233102.7

3_0301 2_1530FGX_OWB00021 2_06851_1437 3_09961_0249 2_1501

103.9

FGX_OWB00354 bPb-5296bPb-9912 bPb-2561bPb-1847 bPb-7399bPb-6386 FGX_OWB00437

114.8

2_1201 lks2118.6ABC1024 3_1188122.43_0335 2_0808123.62_0611 bPb-1079[222171] 2_0103FGX_OWB00044 3_0168

124.9

3_1440 1_1051[220920] FGX_OWB001043_1294

126.1

2_1464 1_0169128.6bPb-4191129.82_0824 FGX_OWB00143Bmag0120131.1

1_0362 2_0406DsT-30132.3

WG380B133.5bPb-2433 1_0563bPb-0027 bPb-9908FGX_OWB00405

138.7

[105081] FGX_OWB00136FGX_OWB00094 bPb-4272139.9

ABC310B FGX_OWB000643_0164 FGX_OWB001451_1184

143.7

FGX_OWB00124 bPb-8498144.9FGX_OWB00316 bPb-61863_1232 1_1243bPb-4389 Ris442_0217

146.2

2_0354 3_15353_1483151.3

1_1279 2_1209FGX_OWB00144 2_1229159.2

FGX_OWB00133 [221079]160.4FGX_OWB00283163.01_0861 FGX_OWB00054FGX_OWB00048 ABC253164.2

1_0797 bPb-7739FGX_OWB00049 3_0831170.7

3_0380171.93_1282 bPb-6167bPb-8644 3_1241bPb-0889 FGX_OWB00499bPb-0758 bPb-5923bPb-1556 bPb-0917ABG461A bPb-43941_0973 [222159]

173.2

3_1491 bPb-0981bPb-7983 1_05502_1280 [220949]bPb-3980 bPb-16313_1325 2_0753FGX_OWB00067 FGX_OWB00027

177.0

2_1363 1_08432_0414 3_11662_1223 DsT-69[222528] WG380A2_0452 1_14403_0593 bPb-8833

178.2

FGX_OWB00019 2_0640179.4bPb-2121 1_0130bPb-3232 bPb-9948[223928] bPb-5898

180.7

FGX_OWB00425181.11_0896 1_13091_1275 2_0962[221299]

181.9

3_1489 FGX_OWB00277FGX_OWB00361183.1

GBM1065 FGX_OWB00234FGX_OWB00235 3_0244184.4

[223404]185.6FGX_OWB00091195.83_1019 bPb-9884FGX_OWB00347 1_0378KFP255 bPb-0328

201.5

ThA1204.0

7H

bPb-6318 FGX_OWB00503bPb-7112 BCD14342_0275

0.0

1_0460 FGX_OWB004911_13112.5

FGX_OWB000863.3DsT-66 bPb-01811_04103.7

1_06365.01_1011 3_09336.2bPb-9608 bPb-7137bPb-7043 bPb-1318Act8A

7.4

2_12269.9[220835] RbgMD3_1533 [222208]3_0952 [105102]3_0950 3_09193_0817

13.7

[222789]15.0bPb-7889 MWG837B17.53_0955 ABC165CFGX_OWB00106 FGX_OWB00146FGX_OWB00046 scind00046

21.3

FGX_OWB00258 [223986]22.5Bmac039925.03_0876 GBM100726.2[105118] 2_07123_0241 bPb-2166FGX_OWB00114 1_0186

30.0

BCD098 [221879]32.53_127633.8bPb-9418 bPb-4813bPb-321737.6

1_0760 [104834][104921] FGX_OWB0016742.7

GBM1042 [221425]1_081443.9

FGX_OWB0020845.3bPb-5672 1_1357bPb-1535 bPb-8662bPb-1398 1_02381_1498 bPb-1231bPb-9388 bPb-15863_0336

46.4

bPb-0357 bPb-51642_0514 bPb-04821_1266 bPb-2175bPb-1604 3_0268[222328] [221108]

47.6

1_029451.4FGX_OWB0020052.01_0438 1_0938[223055] 1_1336bPb-0579 2_10001_1169 1_1287bPb-9360 2_04271_1036 2_13572_0047 FGX_OWB002493_0562 1_13013_1191 2_0757Bmag0211 3_0436FGX_OWB00391 3_1134[221321] 3_04623_0258 2_13123_1381 2_09123_0762 1_12092_1408 2_08102_0698 3_0498

52.7

3_0774 1_1107FGX_OWB00389 3_052253.9

1_1217 1_13592_1217 FGX_OWB0037656.4

bPb-1193 3_02431_1256 FGX_OWB002821_0293 bPb-1791BG369940 3_0348bPb-3382 3_07101_0198 3_0741

57.6

FGX_OWB00315 3_034358.91_0768 2_1333FGX_OWB00023 1_144460.1

2_1463 bPb-17233_1532 bPb-90051_0201

62.6

1_1049 3_07441_0617 3_030463.8

1_0302 FGX_OWB002162_113165.1

3_1401 3_117966.3bPb-1133 [222086]2_1431 2_0642bPb-8183

67.5

bPb-3389 bPb-53393_0295 1_13671_0002 1_1062

72.7

ABC16073.91_0166 3_0298FGX_OWB00418 3_05051_0463 2_0696

75.1

bPb-8713 bPb-61172_1176 3_07421_1075

76.4

1_126777.62_0121 1_02791_089078.9

[224235]80.11_046686.6FGX_OWB00063 3_0072bPb-8477 FGX_OWB0047991.7

1_146395.53_0204 FGX_OWB001051_114496.8

FGX_OWB00372 FGX_OWB002761_053599.3

FGX_OWB0000999.81_1008 JS10C1_1439 FGX_OWB003942_0769 2_04753_1192 bPb-7963

100.5

1_1277 FGX_OWB00449FGX_OWB00515101.7

2_0754 2_0169FGX_OWB00278 FGX_OWB00285FGX_OWB00193 bPb-2007

104.2

bPb-7429 2_10202_0909 FGX_OWB003341_0111 2_0613[221687] 2_0187MWG706A FGX_OWB00178bPb-1419 bPb-91801_1173

110.7

3_0191117.22_0780 KFP170[223108] 2_0921118.5

FGX_OWB00113 FGX_OWB00485[221039] bPb-91082_0625

119.7

2_0021122.2bPb-2063 bPb-50143_1526 bPb-5198124.7

1_0905 FGX_OWB00359FGX_OWB00525127.2

1_1409128.41_0854 1_03383_0257 [221902]FGX_OWB00045

130.9

FGX_OWB00256132.22_1172134.73_1105 1_0339FGX_OWB00239135.9

1_1457137.1MWG2028 1_0207140.91_0808 3_1387bPb-5090 ABC261142.2

3_0649 bPb-6065KFP257B bPb-95521_0693

143.4

bPb-6502 1_0903145.92_0383148.41_1271 3_0803149.62_1105 WMC1E8FGX_OWB00125 FGX_OWB00426FGX_OWB00090

152.1

1_1509 1_13201_0041 MWG912154.6

2_0594155.9FGX_OWB00031157.13_1081 bPb-2260bPb-5550 2_0840ABG387A bPb-14873_0048 FGX_OWB00197bPb-3756 scssr04163

158.3

1H

[223556]0.0FGX_OWB00196 bPb-8959FGX_OWB00441 1_1407bPb-7151 bPb-0516[220862] FGX_OWB00098FGX_OWB00453 bPb-5191bPb-3598 FGX_OWB00455bPb-0611 bPb-36081_0970 3_1446[222367] bPb-7892bPb-0485

1.3

FGX_OWB004632.0FGX_OWB004612.52_1466 ABG0583.71_0718 [222211]scind026225.0

2_14156.2[223937] FGX_OWB00005bPb-0205 bPb-5033bPb-7969 bPb-0471FGX_OWB00496 1_1030FGX_OWB00147 bPb-5900

7.4

bPb-0615 bPb-5688[223539] bPb-7557bPb-5519 bPb-7024[221144]

9.9

1_1059 1_023012.4FGX_OWB00138 [223312]13.7[221686] 3_0155bPb-6897 [220974]bPb-4481 bPb-9220bPb-4295 FGX_OWB00263bPb-6128 3_06313_0775 bPb-0715bPb-1708 FGX_OWB00403bPb-8399 bPb-5444FGX_OWB00061 ABG0083_0402

14.9

bPb-4715 [222714]bPb-2108 [104949]bPb-2355 bPb-8986[221518] [222296]bPb-6879 2_1423bPb-6494 bPb-8374bPb-2782

16.1

2_0724 FGX_OWB00507FGX_OWB00012 bPb-829217.4

1_104019.9bPb-4875 bPb-9682bPb-4261 scssr102262_1261

42.6

2_059343.91_0307 scssr07759bPb-2501 bPb-79062_0368 1_0891bPb-3190

45.1

FGX_OWB00480 2_104946.31_1304 GBM106647.6FGX_OWB00289 FGX_OWB00412FGX_OWB00073 1_084748.8

bPb-2230 2_14613_0420 1_05251_0342 FGX_OWB004832_0326 FGX_OWB00436FGX_OWB00522 FGX_OWB00129Pox 1_08371_1057 1_0178bPb-0722 3_06571_0919 FGX_OWB003631_0296

51.3

1_0084 1_149352.53_0379 FGX_OWB00118FGX_OWB00324 1_0715FGX_OWB00255 3_0029

53.8

1_0486 3_049155.02_0281 1_02041_134056.3

1_0498 bPb-50571_1505 FGX_OWB00287bPb-9925 FGX_OWB00338

58.8

FGX_OWB0020159.43_0691 2_10053_0604 1_0156bPb-0220 [223952]2_0688 1_14282_0674

60.0

1_0422 1_1302[222438] 2_0929[221628] scssr033811_1131 1_0638

61.2

1_1522 3_14741_140063.7

2_0748 2_03872_0602 2_0234bPb-3056 2_02352_0917 FGX_OWB004193_0195 1_0147bPb-6438 1_0927FGX_OWB00505 bPb-68813_0251

66.2

2_0004 1_05583_0209 3_04873_0557 2_0643Hot1

67.4

FGX_OWB0018968.03_1218 [220842]3_0772 2_1286FGX_OWB00107 2_0378bPb-1836 2_0500FGX_OWB00220 [222433]1_1211 1_04742_0878 2_1337FGX_OWB00175 2_00393_0206 2_14342_0181 1_09471_0330 2_0458[220925] 3_08532_0417 2_00323_0634 1_1369FGX_OWB00439 3_11891_0485 1_01541_0099 1_08833_0068 2_01961_1288 FGX_OWB00346scssr02236 Ebmac06841_1155 FGX_OWB001211_0948

68.7

1_138469.92_0335 FGX_OWB00401FGX_OWB0028071.1

FGX_OWB0012271.7scssr00334 FGX_OWB001003_024572.4

1_0436 FGX_OWB00313FGX_OWB00365 FGX_OWB000721_0632 1_0640

73.6

ABG356 3_12563_1252 2_14762_0442 1_13161_1430 2_0374

74.8

bPb-4837 GBM1023bPb-6437 bPb-72563_1307 2_10941_1072 1_1347bPb-0885

76.1

bPb-9754 [222771]bPb-4040 1_1096bPb-6088 bPb-5440FGX_OWB00227

78.6

2_0833 1_0407bPb-7243 bPb-81003_0205 3_1021

82.4

2_0528 2_066783.61_1402 FGX_OWB0000384.8FGX_OWB00079 3_139486.13_017888.6scsnp03343 [222968]91.13_1445 1_138892.31_0859 FGX_OWB004311_078694.8

1_110097.33_0901 1_15333_0897 3_0900vrs1 FGX_OWB003372_0340

98.6

1_0936102.41_0969 2_1351103.62_0793 Bmag0125FGX_OWB00508 FGX_OWB00026104.8

FGX_OWB00007107.3DsT-41 FGX_OWB00273109.8MWG503112.3bPb-4835 bPb-1926FGX_OWB00074 1_1285bPb-6194 FGX_OWB000221_1307 2_1007bPb-7991 GBM10623_0216 bPb-3563

113.6

KFP203114.82_0923 FGX_OWB00526MWG882A 1_03983_0200

117.3

1_0900 2_15271_1466118.5

3_0095 3_0480119.8bPb-3653 bPb-87371_1323 bPb-4577bPb-1772 3_05553_0049

122.3

1_1094 bPb-6822FGX_OWB00464126.1

FGX_OWB00183 bPb-2481FGX_OWB00209 bPb-82742_0955 bPb-38703_1402 [223776]FGX_OWB00060 1_09901_0128 2_0064ABG072 2_1238FGX_OWB00299 1_09891_1043 FGX_OWB000471_0263

132.6

3_0459 1_0707FGX_OWB00055 1_04292_0989 1_1236

133.8

1_0739 FGX_OWB00173137.61_0780 3_1406140.1bPb-8949 bPb-1266141.3bPb-0541 2_13153_0152 [221435]bPb-6688 3_00971_1365 bPb-79922_0141

143.8

[222960] 3_0695Ebmc0415 1_04463_0310 2_1459FGX_OWB00123 1_1486FGX_OWB00238 3_1100FGX_OWB00181 1_01093_0041 FGX_OWB003432_1406

146.3

bPb-2971 FGX_OWB00011cnx1 bPb-5412147.6

[222464]148.81_0065 1_04723_0678 2_11252_0215 bPb-38581_0383 FGX_OWB001532_1336

151.3

FGX_OWB00198 1_1227153.83_0106 3_0396bPb-1505 [221983]155.0

Zeo1156.33_0248158.82_1181 2_0590160.0[223553]167.9bPb-5619 bPb-1184bPb-1815 FGX_OWB001521_1023 bPb-8530FGX_OWB00229 bPb-1566bPb-4092 bPb-1986bPb-5460 GBM1019bPb-0303 [221407]1_0315 1_0566bPb-7208

169.2

2_0943 FGX_OWB00377170.4FGX_OWB00322 FGX_OWB00312Aglu4 FGX_OWB00132Aglu5 FGX_OWB000993_0823

171.6

1_0937 MWG7203_1300174.1

2_0957175.4bPb-4601 bPb-7212bPb-1611 FGX_OWB00004bPb-4232 bPb-1181FGX_OWB00294

177.9

1_0329 GBM1012180.41_1050 FGX_OWB00207bPb-0305 bPb-9419bPb-6296 bPb-60481_0085 bPb-1085bPb-3102 [223337]bPb-8698

182.9

2_1414 FGX_OWB00308184.12_1496185.3FGX_OWB00293186.63_0102 FGX_OWB00408MWG949A FGX_OWB00471scssr08447

187.8

2H

3_1428 bPb-40222_0858 2_00900.0

FGX_OWB002261.2[221259]2.5bPb-2553 [223116]2_0797 [224140]bPb-3815 bPb-4895

3.7

1_14346.22_0252 3_1409FGX_OWB00223 1_1453FGX_OWB00162 FGX_OWB004002_0953 bPb-1264

10.0

bPb-368912.51_1005 3_0297bPb-9945 2_10272_1398

13.7

1_1310 3_09102_0976 1_088615.0

3_0915 FGX_OWB00469[221362]16.2

[221142] [223396][223877] [220882]17.4

FGX_OWB00261 FGX_OWB000753_011319.9

bPb-9745 2_14102_017221.2

bPb-7448 2_07421_0565 [221551]bPb-0654 [221552]

22.4

bPb-0663 bPb-3229bPb-0548 bPb-3865bPb-4824

24.9

GBM1074 2_098228.73_019229.92_055231.22_0794 1_05591_0691 3_092032.4

[221274] 1_1237MWG798B [220909]3_0284 2_04552_0968 2_1402

34.9

bPb-5289 FGX_OWB00182[105024] bPb-9961bPb-2929 bPb-9903

36.2

1_0026 2_0607Dst-27 3_05713_0431 1_1527bPb-3030 2_1268

38.7

bPb-6275 1_0968[220886] bPb-2324bPb-7938 bPb-9129BCD706 [222320]bPb-2993 FGX_OWB00517bPb-2910 [222615][221166] bPb-58921_0081 [222411]1_0598

42.5

3_1159 3_09533_129845.0

FGX_OWB00210 bPb-3332bPb-015847.5

FGX_OWB0022150.0FGX_OWB0014151.23_0913 2_1506FGX_OWB00248 1_11172_1259 FGX_OWB00393

52.4

3_0609 2_118954.9bPb-4407 FGX_OWB004452_0264 FGX_OWB00237FGX_OWB00380 1_10693_0467

56.2

3_0583 3_07211_110257.4

bPb-0068 bPb-67713_1015 [224015][223843] 3_0922FGX_OWB00127 1_13873_0923

58.6

3_0680 1_1511alm FGX_OWB002971_0233

59.9

2_086661.11_1265 3_02623_1008 FGX_OWB00030FGX_OWB00429 1_0391

62.3

2_0591 1_09252_0486 3_15023_0039 1_10993_0130 2_11472_1472 1_12952_0408 FGX_OWB004162_1435 2_08011_1125 bPb-86661_1337 1_1530FGX_OWB00056 FGX_OWB000682_0721 1_0784FGX_OWB00399 2_04392_1411 2_0583FGX_OWB00300 1_12831_1482 Bmac02092_1062

63.6

1_0011 2_044464.8bPb-649166.01_1429 1_06531_0850 2_0108FGX_OWB00130 2_03252_1064 2_15111_0678 ABC325

67.3

DsT-6771.11_1191 2_0863bPb-475772.3

bPb-3805 2_0608bPb-6923 1_1152FGX_OWB00527 1_13422_1502 1_1284bPb-0040

73.5

2_1305 3_115377.31_1394 bPb-0079FGX_OWB00082 1_0200FGX_OWB00267 bPb-8410bPb-3317 1_1314

78.6

FGX_OWB0047079.33_075479.83_1529 1_04522_069481.0

1_0683 3_03993_0743 2_06952_0521 2_01241_0609

82.3

3_134683.51_0047 scssr25691bPb-7872 FGX_OWB00161[223370] 2_03621_1454 FGX_OWB004232_0597 2_1194

86.0

2_0849 2_11632_0115 ABG3771_1524

87.2

FGX_OWB00110 1_151789.72_0063 1_113893.5Bmag022594.83_0663101.33_0325103.81_0583 2_0659105.01_1021 FGX_OWB000181_0747107.5

FGX_OWB00043 FGX_OWB00117FGX_OWB00015 3_1367111.3

ABG499 3_0090FGX_OWB00230115.1

bPb-6722 3_06402_0130116.4

[221404] 1_0184117.62_1083 FGX_OWB002042_1381118.8

3_0342 2_14951_0662121.3

FGX_OWB00292 2_1493122.63_0119 [223016]2_0009123.8

FGX_OWB00342 2_1161FGX_OWB00111 2_0023bPb-9336 1_13922_1081

125.0

2_1277128.83_0375130.13_1329131.31_0584133.83_0928 scsnp009401_0680135.0

bPb-4209 bPb-3630136.31_1330140.1bPb-9110 2_09443_1269 bPb-1579bPb-1961 bPb-7695

141.3

1_0918 [223788]2_1405143.8

3_0274 bPb-7785FGX_OWB00180 bPb-4386145.1

1_1338 1_08213_0084 1_07543_0924 3_1525

146.3

2_0413 3_1238147.53_0507148.83_0081150.01_1196 1_0505ABG004 2_06622_0369 2_03431_1141

152.5

1_0842 2_0612153.73_0973156.2FGX_OWB00510157.0FGX_OWB00502 FGX_OWB003452_0527 3_0092157.5

scind02281162.6bPb-8894 bPb-57962_0085 1_13283_0860 bPb-8506

163.8

FGX_OWB00250 3_1496FGX_OWB00101 bPb-4830166.3

FGX_OWB00272167.63_0137 MWG883FGX_OWB00033168.8

bPb-5129 2_1386bPb-2737 bPb-3109bPb-0049 bPb-8021FGX_OWB00008 bPb-7833

173.9

FGX_OWB00257 FGX_OWB00495FGX_OWB00163175.2

bPb-4739 bPb-38991_1297 bPb-6228FGX_OWB00126 FGX_OWB00040bPb-2420 FGX_OWB00097FGX_OWB00392 FGX_OWB00137bPb-1253 bPb-3843bPb-4748

176.4

bPb-0789 3_1161178.9bPb-7413 FGX_OWB00169FGX_OWB00081 [222583]2_1531

180.1

2_0421182.6FGX_OWB00139 FGX_OWB002512_1345 bPb-18291_1436 bPb-4456FGX_OWB00493 3_0370HVM62 bPb-6631bPb-0302

183.9

bPb-0200 FGX_OWB00281186.41_0935 FGX_OWB004622_1237 1_07023_0960 3_07672_1008 FGX_OWB005183_0271 FGX_OWB00513

190.2

ABC805 2_0605192.71_0629193.9bPb-4169 bPb-4387195.13_1388 FGX_OWB00205bPb-1411 FGX_OWB003841_0681 1_0893

196.4

[222092] [222593]1_0014197.6

1_1516 FGX_OWB00270FGX_OWB00369 bPb-0164200.1

3_0229 2_0176201.33_1341202.62_1500203.83_0055205.0FGX_OWB00489205.72_1387 bPb-5256bPb-5346 FGX_OWB00228FGX_OWB00303 bPb-8962bPb-7724 ABC172bPb-3295 FGX_OWB004421_0343 FGX_OWB00468[221492] 3_0135FGX_OWB00058 bPb-0136[223314] bPb-7120[221662] 1_0215[221355] 3_1296

206.3

[221563]207.6

3H

2_11170.0bPb-7534 bPb-9406bPb-6938 3_13241.2

MWG6343.7FGX_OWB00432 2_0274FGX_OWB00001 3_01403_1486

6.2

1_148514.11_0490 3_145815.43_054021.9[222436] MWG0773_015023.1

1_0574 [104902]FGX_OWB00078 1_0223HVM40 FGX_OWB002192_0557

25.6

3_1291 1_022126.91_119928.11_113629.32_145830.6[222526] 1_10602_0422 1_11752_1385 1_06263_0394 1_0562

31.8

2_1418 FGX_OWB002322_0777 CDO5422_0109 2_0680

33.0

scsnp0164833.43_0907 CDO1221_003134.3

[222439] FGX_OWB0029135.53_116436.7hvknox3 3_1439FGX_OWB00053 1_0810FGX_OWB00284 3_15242_1389

38.0

1_0171 1_0347FGX_OWB00119 3_099240.5

[223373] bPb-64042_0240 2_00111_0371 2_01801_0063 bPb-6611

41.7

3_0187 scssr205691_0048 ABC30344.2

1_0668 1_0793CDO79548.0

1_1112 2_14902_093950.5

1_0756 [223751]3_0450 2_08532_0496 3_13821_0492 2_05252_1071 [220846]3_0525 DST-462_0269 1_09421_0577 3_13601_0432 2_14422_0426 2_10731_1190 3_1026FGX_OWB00378 scind037512_1029 2_02891_1332 2_13031_0261 FGX_OWB000522_0831 3_03311_1042 bPb-95041_0946 1_0607[222294] FGX_OWB003303_0777 FGX_OWB003983_1156 3_08661_0411 1_0667scind04312a 2_11373_0488 FGX_OWB00279

53.0

3_042754.21_0088 2_14811_1232 3_00341_0637 Tef21_0881 3_00602_0412 1_0568FGX_OWB00307 scind01728FGX_OWB00358 1_02623_0839 2_06103_1297 2_12131_0426 [105086]1_1108 2_03631_0527 2_0898

55.5

FGX_OWB0013456.0FGX_OWB0022256.72_1010 2_14001_0086 2_03612_0151

59.2

2_0453 3_07061_1427 bPb-99071_1207 FGX_OWB002252_1296 bPb-6872bPb-2305 2_1273bPb-6949

61.7

GBM1020 2_0820bPb-4290 bPb-6259bPb-7987

62.9

2_0924 1_00522_0062 Bmag03531_1431 1_06392_0098 [224001]2_0723 1_0053

64.2

1_1070 3_045565.41_060666.6bPb-4214 scind10455[221608]71.8

2_1504 1_04671_151373.0

3_0994 bPb-8013bPb-009874.2

3_1258 3_136275.52_1499 2_01432_0207 FGX_OWB003411_1319 1_0309bPb-3556

76.7

FGX_OWB0015577.92_133280.41_1500 FGX_OWB000623_0787 1_0170scssr14079 3_0226

82.9

bPb-3739 1_1526bPb-7719 bPb-6096ABG472 bPb-9998

84.2

1_1213 1_07231_0670 FGX_OWB004062_0197

85.4

bPb-4750 GBM1059bPb-1999 2_0765bPb-0610 bPb-00181_1398

86.6

3_013889.12_073292.91_1168 3_02323_014294.2

1_0785 bPb-5245KFP22195.4

Ebmac070197.93_0584 1_1292FGX_OWB00050 2_07623_0046 3_0117MWG652B 2_08383_0158 1_0666FGX_OWB00160 FGX_OWB002033_0114 FGX_OWB00413FGX_OWB00029

99.1

1_0334 FGX_OWB00482FGX_OWB00382 FGX_OWB001091_1139 FGX_OWB00224FGX_OWB00321 3_0990FGX_OWB00246 2_04542_1111 3_1139

101.6

GBM1048 bPb-9859FGX_OWB00323 [223032]bPb-1166

104.1

1_1194 FGX_OWB00440FGX_OWB00112106.6

3_0385107.9bPb-2677 1_07121_1233 2_0237110.4

[220875] 2_07011_1235 bPb-1040111.6

2_1035 1_10662_0883112.9

3_0146 [223550]2_1130 1_0697Hsh

114.1

HVM67 2_0224115.32_0760116.63_0873 3_04763_0888 2_00073_0889 2_02723_0886 2_14501_0610 3_08843_0808 bPb-34683_0892 3_0887VRN-H2

117.8

[223334]118.22_0932 3_00203_0239 KFP241.1119.0

3_1422120.3ABG601 bPb-5265bPb-9668 3_08243_0825

126.8

4H

bPb-0170 3_01630.0bPb-6051 FGX_OWB00335bPb-6485 2_05531.2

3_09843.73_1023 3_09793_1022 3_00013_0977 3_0975

5.0

2_122111.5scssr0230614.0MWG61821.92_053324.43_109425.62_0010 ABC48326.9ABG610 3_071428.1bPb-2872 2_136529.4bPb-2460 bPb-0091bPb-6568 bPb-1807bPb-7407 bPb-3830bPb-0351 2_1065bPb-8072 2_08733_0167 FGX_OWB00025

35.9

1_097437.13_0531 2_076138.3FGX_OWB0031839.6bPb-2266 bPb-516640.8bPb-0536 2_057642.01_058045.82_0980 2_08452_139148.3

ABG395 1_131849.6FGX_OWB0029850.1bPb-2273 bPb-8589bPb-4135 bPb-42731_0864

50.8

1_1432 FGX_OWB000513_1492 3_0105scssr02503

58.7

2_1308 [222988]1_0481 FGX_OWB003813_0728 1_01771_0252 3_01092_1401 bPb-86752_0697 2_14471_0318 3_1317bPb-1046 1_01571_1469 2_10113_0354 3_1520FGX_OWB00430 bPb-89292_0481 3_07292_0461 3_12572_0524 2_10402_1116 FGX_OWB002413_0709 [221402]2_1350 1_0240[223293] bPb-09493_1423 bPb-5584FGX_OWB00288 bPb-0786bPb-4210 2_07001_0995 1_08563_0768 3_07921_1462 1_13991_1198 FGX_OWB00179FGX_OWB00301 3_03372_0841 2_0101FGX_OWB00006 2_0179FGX_OWB00041 FGX_OWB001481_0913

59.9

FGX_OWB0017760.7bPb-5506 FGX_OWB001681_1260 bPb-7881bPb-2762 1_1204bPb-5727

61.2

3_021462.42_0396 2_0059FGX_OWB00166 FGX_OWB0024363.6

2_0105 3_1117FGX_OWB00214 bPb-7933FGX_OWB00151 2_15361_0264

64.9

3_1062 3_03121_1240 2_03061_1512 3_10643_1340 3_0111[221802] 1_13633_0538 1_02372_0981 1_11513_1183 FGX_OWB001762_0501 3_1451bPb-0503 1_0899

66.1

1_152367.33_1032 1_07253_1034 NRG045A1_1089 3_1035

68.6

bPb-6260 FGX_OWB00039FGX_OWB00038 [223210]3_0745 scsnp04260bPb-7627

69.8

bPb-8987 Ale[224132] 2_103671.0

2_0278 FGX_OWB00159[222177] 3_00801_0203 1_14381_0641 [223812]2_0392 [223146]3_0966 1_1249

74.8

1_054881.33_0719 FGX_OWB002592_029782.6

1_095383.82_0818 bPb-7763bPb-9618 bPb-06863_0252 2_0330bPb-4067 [221220]bPb-2147

87.6

1_030590.12_1133 FGX_OWB004591_0127 2_1128ABC302 1_0183FGX_OWB00084

93.9

2_0246 FGX_OWB000852_0096 1_01671_0633 FGX_OWB005041_0140 scind16991FGX_OWB00002

96.4

1_1515 2_00662_073697.6

scssr1533498.92_1186 3_0314101.41_1135 FGX_OWB00351FGX_OWB00057 scsnp06144105.2

1_0674110.31_0524114.11_0930115.3FGX_OWB00190 1_0578116.61_1473 1_05181_0771 2_0449FGX_OWB00266 srhFGX_OWB00184 2_00973_0834 1_11343_0533

117.8

[105044] bPb-5532bPb-9032 bPb-2869119.0

3_0182120.3FGX_OWB00435124.61_1350126.63_0098 2_0403127.92_0134129.11_0024130.33_0846 FGX_OWB004883_0854 3_08501_0844 3_08472_1321 1_0834HvCBF9 3_0852

131.6

3_1236 3_07052_0805 1_0507FGX_OWB00013 1_08713_0807

134.1

FGX_OWB00414 bPb-73953_0456 3_00561_0477 bPb-5596FGX_OWB00326

136.6

RSB001A137.13_0619 1_1341FGX_OWB00017137.8

[221888] scsnp00177[222196] 1_1245FGX_OWB00164

145.7

FGX_OWB00268 0SU-STS1148.21_1298149.41_1507151.92_1422 FGX_OWB00142bPb-0710 bPb-6135FGX_OWB00390 FGX_OWB00379FGX_OWB00188 FGX_OWB00498

153.2

2_0405 2_0045154.41_1456 [104960]155.6ABG003B156.41_1535 3_06112_0300 3_01691_0228

156.9

1_1472 FGX_OWB00415160.7FGX_OWB00253 1_0755bPb-4084 [221423]2_0551 bPb-2960FGX_OWB00102 [223247]3_1465

170.0

FGX_OWB00158175.2bPb-6578 [222215]bPb-5845176.4

Tef3177.61_0004178.93_1182 1_01043_1234 2_07312_0830 FGX_OWB00024FGX_OWB00262 2_0676

180.1

2_0100 MWG8771_1441 3_00622_0388 3_1206FGX_OWB00192 1_00803_1050 FGX_OWB003192_1462 1_0333

181.3

3_1221 3_0795[223182]183.8

[105067] 3_08302_0104185.1

2_0186186.31_1497187.51_0582 2_0317FGX_OWB00331 3_0759FGX_OWB00092 FGX_OWB001851_0536 ABG4961_0741 3_0238FGX_OWB00014 1_09021_0901 1_0385

191.3

FGX_OWB00010192.1FGX_OWB00501 FGX_OWB000662_0560 2_0876192.6

FGX_OWB00521 2_1018FGX_OWB00512 1_02733_0642 3_13751_0336 1_14642_0646 FGX_OWB00356FGX_OWB00306 1_1325

195.1

FGX_OWB00187201.3[222383] 2_0334bPb-9147 bPb-6195202.8

E10757A scsnp021092_0829205.3

2_0826 3_0666206.5bPb-3138 ABG3911_1216 [221131]FGX_OWB00156 bPb-0618

209.0

FGX_OWB00524 3_00871_0769 JS10B2_0546

210.2

bPb-6179 bPb-4595bPb-0835212.8

[104899]214.01_0778215.21_0600 bPb-0877[223803] bPb-4621[222906] [223339][222776]

216.5

[224041]217.01_1450 3_1404ABC622 1_02541_1010

217.7

bPb-9132 3_04941_0736 2_01893_0769 3_0504DsT-33 bPb-7421FGX_OWB00035

220.2

bPb-9868224.02_1393226.5bPb-8754 FGX_OWB000361_0310227.7

3_1292 bPb-62391_1364 FGX_OWB002442_1108 bPb-1217[222075]

231.5

1_0732 FGX_OWB002401_1413 3_14811_0020

234.0

bPb-2419 bPb-7008FGX_OWB00340 FGX_OWB00206bPb-3973 MWG602A[221531]

235.3

3_0958 bPb-6124bPb-7360 bPb-5766bPb-7292 1_03222_0402 scssr03906bPb-4725 3_11232_1356 bPb-9244

236.5

[221535]237.8

5H

[223608] 2_02323_0319 bPb-37800.0

FGX_OWB00325 FGX_OWB00360FGX_OWB003861.2

FGX_OWB001991.9[222141] FGX_OWB00154[223456] Bmac0316bPb-7323 bPb-70302_0881

2.5

bPb-9768 2_08823.71_11664.3bPb-9075 [223070]bPb-3532 bPb-10012_0294 2_12042_0262 bPb-8275[221458] bPb-6511

4.9

FGX_OWB00202 bPb-3807bPb-71936.2

bPb-5498 bPb-63113_0010 bPb-3433[222316] bPb-0572[222053] FGX_OWB00269bPb-4778 FGX_OWB00486[221961]

20.1

3_084222.6FGX_OWB0039523.9[224154] bPb-849226.41_0064 MWG602B27.6[223325] [223374]2_1246 bPb-71651_0023

28.8

bPb-9749 bPb-6661bPb-5252 2_031532.6

[221152] [222286]36.4FGX_OWB0023337.7[223808] FGX_OWB00242[220998]38.9

bPb-3921 1_0774JS10A 2_074540.1

3_069741.43_067342.63_130843.81_0994 GBM1021FGX_OWB00120 1_07991_0939 FGX_OWB00076

46.3

bPb-6002 [223386][221169] bPb-3927bPb-0597 1_0427

47.6

FGX_OWB00089 bPb-7324[220812]48.8

3_052152.6[221710]53.93_036155.11_0061 1_01291_1455 3_078356.3

GBM106857.1[223273]57.52_0936 [220818]bPb-5379 FGX_OWB0011658.8

3_0317 3_1274FGX_OWB00077 1_04611_0575 1_09103_0281 1_0539BG299297 FGX_OWB002602_0983 1_08172_0707 1_01991_0811 1_03553_1092 FGX_OWB00336FGX_OWB00327 bPb-1466FGX_OWB00186 bPb-6142

60.0

2_0675 [223115]bPb-5270 1_11713_0782 bPb-25921_1205

61.3

2_144962.53_0569 FGX_OWB00476HVM3163.7

2_0651 1_09622_0381 2_11583_0120 bPb-91142_0601 1_1353rob 3_10073_0021 3_0032bPb-6567 bPb-16662_0720 bPb-34873_1004 2_06561_0497 1_11042_1014 2_1473

65.0

1_0954 1_0749[223262] [223845]3_0765 [223825]1_1140 1_14421_0848 [223671][223028] 2_07991_1487 3_15091_0591 [221391]3_1443 [222899][223002] 3_08372_0785 2_08543_1249 1_1312[223658] [222912]scssr02093 FGX_OWB002123_1187 1_06593_0567 bPb-25933_0230 [224253]2_1216 2_0946[222814] 2_01421_0323 Bmag00092_0567 1_1153FGX_OWB00494 3_0305[223207] 3_05082_0572 FGX_OWB00290[224033]

66.2

bPb-1657 1_02781_0513 3_11742_0202 bPb-97021_0345 FGX_OWB002132_1114 FGX_OWB00059bPb-5196 bPb-00192_0835 [221874]1_1181 [222527][222484] [221245]

67.4

bPb-9835 bPb-9082bPb-905168.7

bPb-4229 1_0803bPb-9479 bPb-551771.2

FGX_OWB00344 bPb-4369ABG474 1_0964bPb-6721 bPb-8347bPb-3230 FGX_OWB001312_0266 3_1003bPb-4409

72.4

bPb-306873.3bPb-1256 1_01891_1321 FGX_OWB00096[224127]

73.6

2_1293 2_12982_0904 2_12251_1261 1_03922_0148

78.8

2_0714 FGX_OWB00218FGX_OWB00020 ABG3881_0504

81.3

2_046882.51_0596 [223211]3_1289 2_06202_0892 2_0673

87.6

FGX_OWB0034988.22_0784 1_1349scsnp21226 3_12502_0577 2_0848

88.9

3_0236 FGX_OWB00028FGX_OWB00171 2_06823_1111 FGX_OWB00088MWG820 2_0488

91.4

3_0573 FGX_OWB0007192.6FGX_OWB00115 3_014893.83_125395.1FGX_OWB00407 FGX_OWB0042197.61_0331100.11_0185 FGX_OWB005141_1246101.3

2_0346 2_1404102.53_0452 1_1147105.0[222895]110.2FGX_OWB00254110.91_0400111.42_1025 1_0202112.63_1235113.93_0932 3_03242_0996 3_1225115.1

2_0972 GBM10081_0595 1_0978117.6

3_1048 FGX_OWB002751_1494 3_10493_1043 [220914][221783] 3_13533_1115 3_10423_1044 1_0139

118.8

2_0379121.3bPb-6477 FGX_OWB00217FGX_OWB00295 FGX_OWB00172bPb-9063

122.6

scind04312b123.82_0036 GBM1022127.6bPb-3470128.8Bmac0040 2_0355136.7[221063] [221088]bPb-5587 2_0448138.0

2_0558 DsT-32B139.23_1198 2_1477148.63_0025 FGX_OWB00069149.8bPb-7446 2_14553_0355 bPb-94131_0071 1_00511_0107 3_13923_1277 1_06452_1467

151.0

3_0057 3_1126FGX_OWB00428 DsT-281_1187

152.3

bPb-5515153.51_1488 FGX_OWB002962_0211 FGX_OWB00080DsT-74 FGX_OWB002861_0748

154.7

bPb-3760 bPb-2940bPb-6677 2_0907bPb-2863 2_0868

156.0

3_1498 FGX_OWB00424FGX_OWB00446 3_1469158.5

bPb-2304 FGX_OWB00174bPb-0649 1_0390159.7

bPb-2410 [222800]FGX_OWB00329 [223186]bPb-9890 bPb-9817

160.9

bPb-0403 bPb-7146bPb-1621 MWG514FGX_OWB00236 bPb-4626bPb-1029 MWG798A1_1111

162.2

[220848]163.4

6H

2_1419 1_00360.0FGX_OWB005090.5FGX_OWB003040.91_0682 3_04721_1132 ABG704FGX_OWB00305

1.2

2_0201 1_01211_08942.5

bPb-37323.1bPb-0844 bPb-8272bPb-0398 bPb-7559bPb-4457 [223152]bPb-7349 bPb-8084[223392]

3.7

FGX_OWB004814.43_13504.9FGX_OWB003175.31_1179 FGX_OWB000956.1FGX_OWB00302 [222156][224158] 1_1031FGX_OWB00032 FGX_OWB00523[221162] [221346]3_1173 bPb-4445

7.4

FGX_OWB000708.01_1035 FGX_OWB00211bPb-25958.6

[223056] [221786]bPb-5816 FGX_OWB002159.8

bPb-9202 FGX_OWB00452bPb-589711.1

2_0227 [222301]bPb-463412.3

2_075513.53_0902 GBSS-I16.0FGX_OWB0030917.43_0723 1_1052bPb-6453 bPb-372718.5

2_1050 FGX_OWB0048719.7bPb-4266 FGX_OWB0016523.5AW982580 FGX_OWB003752_049524.8

FGX_OWB00529 [222485]27.3FGX_OWB0024728.53_0329 bPb-86601_0965 3_0780MWG089 CDO4753_1351

32.3

3_0702 FGX_OWB001281_045133.6

FGX_OWB00170 bPb-0324bPb-1360 bPb-5494bPb-2533 bPb-8939bPb-5074 1_0232[221832] 2_0192bPb-9601 3_00833_0242 FGX_OWB00265bPb-5852 FGX_OWB003742_0993 ABG380bPb-0678 bPb-6156

34.8

2_129242.73_0065 1_097944.0FGX_OWB0050047.81_036849.0FGX_OWB00311 FGX_OWB000931_133951.5

2_149152.82_1326 2_015054.0FGX_OWB0024555.2FGX_OWB0006556.33_0752 3_03711_0772 2_003157.7

bPb-8674 2_011361.53_0290 FGX_OWB0008762.73_018164.0ABC255 3_0880scsnp00460 1_040367.8

FGX_OWB00016 [220928][221740] ABC165DFGX_OWB00448 1_0721

69.0

1_005070.2FGX_OWB00195 3_0639FGX_OWB00083 3_1463FGX_OWB00310 1_06573_0031 2_07502_1270

71.5

bPb-1952 bPb-4219bPb-3561 bPb-795275.3

FGX_OWB00034 FGX_OWB001911_1028 FGX_OWB000372_0060 1_1242

77.8

FGX_OWB0010879.0bPb-1447 bPb-805180.23_1452 FGX_OWB002642_1234 scssr158641_1045 bPb-1093FGX_OWB00352 1_1257FGX_OWB00314 FGX_OWB00149[221655] 2_14923_0997 FGX_OWB00157FGX_OWB00231 1_11031_1202 bPb-5458[221264] [222063]

81.5

1_0983 [222546]2_0885 2_11673_1120 [104913]

82.7

GBM1030 3_034485.21_1519 3_05811_008387.7

bPb-6975 3_05631_0268 3_06211_1145 2_08933_0475 FGX_OWB00042FGX_OWB00135 bPb-51263_0411 2_1178bPb-8956 3_07602_1494 2_0200bPb-2379 1_05813_0445 3_13453_0574 bPb-21883_0389 3_12273_0794 MWG8082_0879 DAK642FGX_OWB00271 FGX_OWB001032_0828 bPb-17701_0055 bPb-55991_0115 bPb-83962_1319 bPb-20971_0698 1_07733_0492 2_03142_1335 3_12151_1352 bPb-79153_0550 3_00041_0924 3_1140bPb-3227 2_13021_1291 1_0073FGX_OWB00150 1_03702_0911 3_0449

88.9

bPb-982591.43_0565 FGX_OWB002521_1029 FGX_OWB003971_1461

95.2

1_1091 2_03493_0199 1_05311_0442 1_06733_1137 FGX_OWB00274scsnp00703 2_08961_1445 2_1330

97.7

2_0771 MWG20312_1409 1_01432_0083

99.0

[221574]100.2bPb-8439 nudbPb-6233102.7

3_0301 2_1530FGX_OWB00021 2_06851_1437 3_09961_0249 2_1501

103.9

FGX_OWB00354 bPb-5296bPb-9912 bPb-2561bPb-1847 bPb-7399bPb-6386 FGX_OWB00437

114.8

2_1201 lks2118.6ABC1024 3_1188122.43_0335 2_0808123.62_0611 bPb-1079[222171] 2_0103FGX_OWB00044 3_0168

124.9

3_1440 1_1051[220920] FGX_OWB001043_1294

126.1

2_1464 1_0169128.6bPb-4191129.82_0824 FGX_OWB00143Bmag0120131.1

1_0362 2_0406DsT-30132.3

WG380B133.5bPb-2433 1_0563bPb-0027 bPb-9908FGX_OWB00405

138.7

[105081] FGX_OWB00136FGX_OWB00094 bPb-4272139.9

ABC310B FGX_OWB000643_0164 FGX_OWB001451_1184

143.7

FGX_OWB00124 bPb-8498144.9FGX_OWB00316 bPb-61863_1232 1_1243bPb-4389 Ris442_0217

146.2

2_0354 3_15353_1483151.3

1_1279 2_1209FGX_OWB00144 2_1229159.2

FGX_OWB00133 [221079]160.4FGX_OWB00283163.01_0861 FGX_OWB00054FGX_OWB00048 ABC253164.2

1_0797 bPb-7739FGX_OWB00049 3_0831170.7

3_0380171.93_1282 bPb-6167bPb-8644 3_1241bPb-0889 FGX_OWB00499bPb-0758 bPb-5923bPb-1556 bPb-0917ABG461A bPb-43941_0973 [222159]

173.2

3_1491 bPb-0981bPb-7983 1_05502_1280 [220949]bPb-3980 bPb-16313_1325 2_0753FGX_OWB00067 FGX_OWB00027

177.0

2_1363 1_08432_0414 3_11662_1223 DsT-69[222528] WG380A2_0452 1_14403_0593 bPb-8833

178.2

FGX_OWB00019 2_0640179.4bPb-2121 1_0130bPb-3232 bPb-9948[223928] bPb-5898

180.7

FGX_OWB00425181.11_0896 1_13091_1275 2_0962[221299]

181.9

3_1489 FGX_OWB00277FGX_OWB00361183.1

GBM1065 FGX_OWB00234FGX_OWB00235 3_0244184.4

[223404]185.6FGX_OWB00091195.83_1019 bPb-9884FGX_OWB00347 1_0378KFP255 bPb-0328

201.5

ThA1204.0

7H

Oregon Wolfe Barley Linkage Map (2383 loci + 463RAD = 2846 loci)

Page 52: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

Homoeology: Chromosomes, or chromosome segments, which are similar in terms of the order and function of the genetic loci.

Homoeologous chromosomes may occur within a single allopolyploid individual (e.g. the A,B, and D genomes in wheat)

Or they may be found in related species (e.g. the 1A, 1B, 1D series in wheat and the 1H of barley)

Map Relationships

Page 53: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

Synteny or syntenic regions: Genetic loci that are linked on the same chromosome

Chromosomes and/or sections of chromosomes are reassembled in different configurations in related organisms

Fragaria: Prunus

Genome Organisation & Maps

Page 54: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

Orthology: Genes in different species which are so similar in sequence that they are assumed to have originated from a single ancestral gene.

doi/10.1371/journal.pcbi.1000703

Genome Organisation & Maps

Page 55: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

Interference and Mapping

Page 56: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

• Interference means that recombination events in adjacent intervals interfere. The occurrence of an event in a given interval may reduce or enhance the occurrence of an event in its neighbourhood.

• Positive interference refers to the ‘suppression’ of recombination events in the neighbourhood of a given one.

• Negative interference refers to the opposite: enhancement of clusters of recombination events.

• Positive interference results in less double recombinants (over adjacent intervals) than expected on the basis of independence of recombination events.

Interference

rAC=rAB+rBC-2CrABrBC

Page 57: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

Interference

C = coefficient of coincidence

A B C

a b c

Interference I = 1 - C

overs cross double ofnumber Expected

overs cross double ofnumber ObservedC

Coefficient of coincidence

For N individuals, the expected number of double crossovers = rABrBCN

Page 58: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

Observed 22 24 16 14 8 10 2 4

24.0100

)42108(ˆ

36.0100

)421416(ˆ

BC

AB

r

r

Coefficient of Coincidence (C)

69.064.8

6

10024.036.0

42

DCs ofnumber Expected

DCs ofnumber Observed

C

Page 59: Linkage Chromosome mapping by recombination Meiosis is the basis of transmission genetics The recombination that occurs during meiosis (in heterozygotes)

Interference

• No interference– C = 1 and Interference = 1-C = 0

• Complete interference– C = 0 and Interference = 1-C = 1

• Negative interference– C > 1 and Interference = 1-C < 0

• Positive interference– C < 1 and Interference = 1-C > 0