JOURNAL OF CLINICAL MICROBIOLOGY

Volume 45 April 2007 No. 4

BACTERIOLOGY

Controlled Evaluation of the IDI-MRSA Assay for Detectionof Colonization by Methicillin-Resistant Staphylococcusaureus in Diverse Mucocutaneous Specimens

Nour de San, Olivier Denis, Marie-Fabrice Gasasira, Ricardo DeMendonca, Claire Nonhoff, andMarc J. Struelens

1098–1101

First Notification of Positive Blood Cultures and the HighAccuracy of the Gram Stain Report

Mette Søgaard, Mette Nørgaard,and Henrik C. Schønheyder

1113–1117

Virulence Gene- and Pandemic Group-Specific MarkerProfiling of Clinical Vibrio parahaemolyticus Isolates

Carolyn E. Meador, Michele M.Parsons, Cheryl A. Bopp, PeterGerner-Smidt, John A. Painter,and Gary J. Vora

1133–1139

Detection of Extended-Spectrum Beta-Lactamases amongEnterobacteriaceae by Use of Semiautomated MicrobiologySystems and Manual Detection Procedures

Irith Wiegand, Heinrich K. Geiss,Dietrich Mack, Enno Sturenburg,and Harald Seifert

1167–1174

International Quality Assurance Study for Characterizationof Streptococcus pyogenes

Shona Neal, Bernard Beall, KimEkelund, Birgitta Henriques-Normark, Aftab Jasir, DwightJohnson, Edward Kaplan,Marguerite Lovgren, Ralf ReneReinert, Members of the Strep-EURO Study Group andInternational StreptococcusReference Laboratories, andAndroulla Efstratiou

1175–1179

Detection of Extended-Spectrum �-Lactamases by UsingBoronic Acid as an AmpC �-Lactamase Inhibitor in ClinicalIsolates of Klebsiella spp. and Escherichia coli

Wonkeun Song, Il Kwon Bae, You-Nae Lee, Chae-Hoon Lee, SangHee Lee, and Seok Hoon Jeong

1180–1184

Identification of Variable-Number Tandem-Repeat (VNTR)Sequences in Legionella pneumophila and Development of anOptimized Multiple-Locus VNTR Analysis Typing Scheme

Christine Pourcel, Paolo Visca,Baharak Afshar, Silvia D’Arezzo,Gilles Vergnaud, and Norman K.Fry

1190–1199

Evaluation of Different PCR Assays for Early Detection ofAcute and Relapsing Brucellosis in Humans in Comparisonwith Conventional Methods

Stella Mitka, Constantine Anetakis,Efimia Souliou, Eudoxia Diza, andAthina Kansouzidou

1211–1218

A Prospective, Randomized, Double-Blind Study of VaginalMicroflora and Epithelium in Women Using a Tampon withan Apertured Film Cover Compared with Those in WomenUsing a Commercial Tampon with a Cover of NonwovenFleece

David J. Chase, Berenike P.Schenkel, Anne-Marie Fahr, andUlrich Eigner for the Tampon StudyGroup

1219–1224

Discovery of a New Capsular Serotype (6C) withinSerogroup 6 of Streptococcus pneumoniae

In Ho Park, David G. Pritchard,Rob Cartee, Angela Brandao, MariaCristina C. Brandileone, and MoonH. Nahm

1225–1233

Identification of “Haematobacter,” a New Genus of AerobicGram-Negative Rods Isolated from Clinical Specimens, andReclassification of Rhodobacter massiliensis as“Haematobacter massiliensis comb. nov.”

Leta O. Helsel, Dannie Hollis,Arnold G. Steigerwalt, Roger E.Morey, Jean Jordan, Tin Aye, JonRadosevic, Deanna Jannat-Khah,Dorothy Thiry, David R. Lonsway,Jean B. Patel, Maryam I.Daneshvar, and Paul N. Levett

1238–1243

Continued on following page

Real-Time PCR Assay for Detection of Quinolone-ResistantNeisseria gonorrhoeae in Urine Samples

Mark J. Siedner, Mark Pandori,Lina Castro, Pennan Barry, WilliamL. H. Whittington, Sally Liska, andJeffrey D. Klausner

1250–1254

A New Phylogenetic Cluster of Cereulide-Producing Bacilluscereus Strains

Maria Vassileva, Keizo Torii,Megumi Oshimoto, Akira Okamoto,Norio Agata, Keiko Yamada, TadaoHasegawa, and Michio Ohta

1274–1277

Survival of Fastidious and Nonfastidious Aerobic Bacteriain Three Bacterial Transport Swab Systems

Nabeel Rishmawi, Raed Ghneim,Randa Kattan, Riyad Ghneim,Madeleine Zoughbi, Afaf Abu-Diab,Sultan Turkuman, Rula Dauodi,Issa Shomali, Abed El-Razeq Issa,Issa Siriani, Hiyam Marzouka,Irmgard Schmid, and Musa Y.Hindiyeh

1278–1283

Detection of Genes Encoding Internalization-AssociatedProteins in Streptococcus pyogenes Isolates from Patientswith Invasive Diseases and Asymptomatic Carriers

Lucilla Baldassarri, Roberta Creti,Monica Imperi, Simona Recchia,Marco Pataracchia, and GraziellaOrefici

1284–1287

Hetero-Vancomycin-Intermediate Methicillin-ResistantStaphylococcus aureus Isolate from a Medical Center in LasCruces, New Mexico

Alejandro Delgado, James T.Riordan, Reena Lamichhane-Khadka, David C. Winnett, JenniferJimenez, Kim Robinson, Frances G.O’Brien, Stephanie A. Cantore, andJohn E. Gustafson

1325–1329

Deletion of fetA Gene Sequences in Serogroup B and CNeisseria meningitidis Isolates

Jane W. Marsh, Mary M. O’Leary,Kathleen A. Shutt, and Lee H.Harrison

1333–1335

Determination of bft Gene Subtypes in Bacteroides fragilisClinical Isolates

Mario J. Avila-Campos, ChengxuLiu, Yuli Song, Marie-ClaireRowlinson, and Sydney M. Finegold

1336–1338

Accuracies of �-Lactam Susceptibility Test Results forPseudomonas aeruginosa with Four Automated Systems(BD Phoenix, MicroScan WalkAway, Vitek, and Vitek 2)

Stefan Juretschko, Vincent J.LaBombardi, Stephen A. Lerner,Paul C. Schreckenberger, and thePseudomonas AST Study Group

1339–1342

High Frequencies of Clindamycin and TetracyclineResistance in Methicillin-Resistant Staphylococcus aureusPulsed-Field Type USA300 Isolates Collected at a BostonAmbulatory Health Center

Linda L. Han, Linda K. McDougal,Rachel J. Gorwitz, Kenneth H.Mayer, Jean B. Patel, Janet M.Sennott, and John L. Fontana

1350–1352

Controlled Clinical Comparison of BacT/ALERT StandardAerobic and Standard Anaerobic Blood Culture BottlesInoculated Directly or after Transport in SodiumPolyanethol Sulfonate Tubes

Brian C. Pien, Stanley Mirrett, BettyR. Crews, L. Barth Reller, andChristopher W. Woods

1357–1359

Optimization of Culture of Leptospira from Humans withLeptospirosis

Vanaporn Wuthiekanun,Wirongrong Chierakul, DirekLimmathurotsakul, Lee D. Smythe,Meegan L. Symonds, Michael F.Dohnt, Andrew T. Slack,Roongreung Limpaiboon, YupinSuputtamongkol, Nicholas J. White,Nicholas P. J. Day, and Sharon J.Peacock

1363–1365

Continued from preceding page

Continued on following page

CHLAMYDIOLOGY AND RICKETTSIOLOGYDistribution Study of Chlamydia trachomatis Serovars amongHigh-Risk Women in China Performed Using PCR-Restriction Fragment Length Polymorphism Genotyping

Xing Gao, Xiang-Sheng Chen, Yue-Ping Yin, Ming-Ying Zhong, Mei-Qin Shi, Wan-Hui Wei, QiangChen, Rosanna W. Peeling, andDavid Mabey

1185–1189

MYCOBACTERIOLOGY AND AEROBICACTINOMYCETES

Evaluation of Microscopic Observation Drug SusceptibilityAssay for Detection of Multidrug-Resistant Mycobacteriumtuberculosis

Girum Shiferaw, YimtubezinashWoldeamanuel, Mekdes Gebeyehu,Feven Girmachew, DanielDemessie, and Eshetu Lemma

1093–1097

Analysis of Multiple Differing Copies of the 16S rRNA Genein Five Clinical Isolates and Three Type Strains of NocardiaSpecies and Implications for Species Assignment

Patricia S. Conville and Frank G.Witebsky

1146–1151

Colorimetric Phage-Based Assay for Detection of Rifampin-Resistant Mycobacterium tuberculosis

Ruth McNerney, Kim Mallard,Honorathy M. R. Urassa, EshetuLemma, and Helen D. Donoghue

1330–1332

MYCOLOGYMulticenter Evaluation of the New VITEK 2 AdvancedColorimetric Yeast Identification Card

D. Jane Hata, Leslie Hall, AnnetteW. Fothergill, Davise H. Larone,and Nancy L. Wengenack

1087–1092

Molecular Identification of Unusual Pathogenic YeastIsolates by Large Ribosomal Subunit Gene Sequencing:2 Years of Experience at the United Kingdom MycologyReference Laboratory

Christopher J. Linton, Andrew M.Borman, Grace Cheung, Ann D.Holmes, Adrien Szekely, Michael D.Palmer, Paul D. Bridge, Colin K.Campbell, and Elizabeth M.Johnson

1152–1158

Five-Hour Diagnosis of Dermatophyte Nail Infections withSpecific Detection of Trichophyton rubrum

Anna Brillowska-Dabrowska, DitteMarie Saunte, and Maiken CavlingArendrup

1200–1204

Multicenter Evaluation of a Commercial PCR–Enzyme-Linked Immunosorbent Assay Diagnostic Kit (Onychodiag)for Diagnosis of Dermatophytic Onychomycosis

C. Savin, S. Huck, C. Rolland, M.Benderdouche, O. Faure, G.Noacco, J. Menotti, E. Candolfi, H.Pelloux, R. Grillot, S. Coupe, and F.Derouin

1205–1210

Molecular Identification of Cryptococcus neoformansSerotypes

A. Enache-Angoulvant, J.Chandenier, F. Symoens, P. Lacube,J. Bolognini, C. Douchet, J. L.Poirot, and C. Hennequin

1261–1265

Systematic Survey of Nonspecific Agglutination by Candidaspp. in Latex Assays

Karsten Becker, Ahmed S. Almasri,Christof von Eiff, Georg Peters,Christine Heilmann, and WolfgangFegeler

1315–1318

Evaluation of Disk Diffusion and Etest Compared to BrothMicrodilution for Antifungal Susceptibility Testing ofPosaconazole against Clinical Isolates of Filamentous Fungi

Shawn A. Messer, Daniel J.Diekema, Richard J. Hollis, LindaB. Boyken, Shailesh Tendolkar,Jennifer Kroeger, and Michael A.Pfaller

1322–1324

Evaluation of VITEK 2 and RapID Yeast Plus Systems forYeast Species Identification: Experience at a Large ClinicalMicrobiology Laboratory

Maurizio Sanguinetti, Rosaria Porta,Michela Sali, Marilena La Sorda,Giovanni Pecorini, Giovanni Fadda,and Brunella Posteraro

1343–1346

Continued from preceding page

Continued on following page

PARASITOLOGYRetrospective Species Identification of MicrosporidianSpores in Diarrheic Fecal Samples from HumanImmunodeficiency Virus/AIDS Patients by MultiplexedFluorescence In Situ Hybridization

Thaddeus K. Graczyk, Michael A.Johansson, Leena Tamang, GovindaS. Visvesvara, Laci S. Moura,Alexandre J. DaSilva, Autumn S.Girouard, and Olga Matos

1255–1260

Milk of Cow (Bos taurus), Buffalo (Bubalus bubalis), andGoat (Capra hircus): a Better Alternative than Fetal BovineSerum in Media for Primary Isolation, In Vitro Cultivation,and Maintenance of Leishmania donovani Promastigotes

M. Muniaraj, C. S. Lal, S. Kumar,P. K. Sinha, and P. Das

1353–1356

VIROLOGYMulticenter Beta Trial of the GeneXpert Enterovirus Assay Christine B. Kost, Beverly Rogers,

M. Steven Oberste, ChristineRobinson, Brenda L. Eaves, KristiLeos, Susan Danielson, MaliniSatya, Fred Weir, and Frederick S.Nolte

1081–1086

Use of Sequence Analysis of the NS5B Region for RoutineGenotyping of Hepatitis C Virus with Reference to C/E1 and5� Untranslated Region Sequences

Donald G. Murphy, BernardWillems, Marc Deschenes, NirHilzenrat, Roger Mousseau, andSidney Sabbah

1102–1112

Epidemiologic Analysis of Reactivated CytomegalovirusAntigenemia in Patients with Cancer

Xiang Y. Han 1126–1132

Improvement of Hepatitis C Virus (HCV) GenotypeDetermination with the New Version of the INNO-LiPAHCV Assay

Francoise Bouchardeau, JeanFrancois Cantaloube, StephaneChevaliez, Christine Portal, AnnieRazer, Jean-Jacques Lefrere, JeanMichel Pawlotsky, Philippe DeMicco, and Syria Laperche

1140–1145

Comparison of the MChip to Viral Culture, ReverseTranscription-PCR, and the QuickVue Influenza A�B Testfor Rapid Diagnosis of Influenza

Martin Mehlmann, Aleta B. Bonner,John V. Williams, Daniela M.Dankbar, Chad L. Moore, RobertD. Kuchta, Amy B. Podsiad, JohnD. Tamerius, Erica D. Dawson, andKathy L. Rowlen

1234–1237

Evaluation of the Performance of the Automated NucliSENSeasyMAG and EasyQ Systems versus the Roche AmpliPrep-AMPLICOR Combination for High-Throughput Monitoringof Human Immunodeficiency Virus Load

W. Stevens, P. Horsfield, and L. E.Scott

1244–1249

Development of Methods for Coordinate Measurement ofTotal Cell-Associated and Integrated HumanImmunodeficiency Virus Type 1 (HIV-1) DNA Forms inRoutine Clinical Samples: Levels Are Not Associated withClinical Parameters, but Low Levels of Integrated HIV-1DNA May Be Prognostic for Continued Successful Therapy

J. M. Carr, K. M. Cheney, C.Coolen, A. Davis, D. Shaw, W.Ferguson, G. Chang, G. Higgins,C. Burrell, and P. Li

1288–1297

Human Herpesvirus 6 DNA Levels in Cerebrospinal FluidDue to Primary Infection Differ from Those Due toChromosomal Viral Integration and Have Implications forDiagnosis of Encephalitis

Katherine N. Ward, Hoe NamLeong, Anton D. Thiruchelvam,Claire E. Atkinson, and DuncanA. Clark

1298–1304

Real-Time PCR Assay Using Specimens on Filter Disks as aTemplate for Detection of Cytomegalovirus in Urine

Naoki Nozawa, Shin Koyano,Yumiko Yamamoto, Yuhki Inami,Ichiro Kurane, and Naoki Inoue

1305–1307

Characterization of Class I Newcastle Disease Virus Isolatesfrom Hong Kong Live Bird Markets and Detection UsingReal-Time Reverse Transcription-PCR

L. Mia Kim, Daniel J. King, DavidL. Suarez, Chun W. Wong, andClaudio L. Afonso

1310–1314

Continued from preceding page

Continued on following page

Significant Genetic and Antigenic Variability within the envGene of Systemic Human Immunodeficiency Virus Type 1Group O Populations during the Natural Course of aHeterosexual Infection: a Pilot Study

Laurent Andreoletti, Brigitte Reveil,Helene Moret, Veronique Brodard,Frederique Philbert, Thierry Tabary,and Jacques Henri Max Cohen

1319–1321

Outbreak of Gastroenteritis Due to Sapovirus Grant S. Hansman, Hiroyuki Saito,Chihiro Shibata, Shizuko Ishizuka,Mitsuaki Oseto, Tomoichiro Oka,and Naokazu Takeda

1347–1349

CLINICAL VETERINARY MICROBIOLOGYMethicillin-Resistant Staphylococcus pseudintermedius in aVeterinary Teaching Hospital

Takashi Sasaki, Ken Kikuchi,Yoshikazu Tanaka, NamikoTakahashi, Shinichi Kamata, andKeiichi Hiramatsu

1118–1125

Worldwide Occurrence of Feline Hemoplasma Infectionsin Wild Felid Species

Barbara Willi, Claudia Filoni, JoseL. Catao-Dias, Valentino Cattori,Marina L. Meli, Astrid Vargas,Fernando Martınez, Melody E.Roelke, Marie-Pierre Ryser-Degiorgis, Christian M.Leutenegger, Hans Lutz, andRegina Hofmann-Lehmann

1159–1166

EPIDEMIOLOGYClonal Distribution of Invasive Neisseria meningitidisSerogroup C Strains Circulating from 1976 to 2005 inGreater Sao Paulo, Brazil

Ana Paula Silva de Lemos, TeresaYkuko Yara, Maria Cecılia OuteiroGorla, Maria Vaneide de Paiva,Adriana Lambert de Souza, MariaInes Cappelletti Goncalves, SamantaCristine Grassi de Almeida, GloriaRegina Feitas do Valle, and ClaudioTavares Sacchi

1266–1273

Molecular Epidemiology of Astrovirus Infection in Infantsin Wuhan, China

Man-Qing Liu, Bei-Fang Yang, Jin-Song Peng, Dun-Jin Zhou, Li Tang,Bin Wang, Yu Liu, Shi-Hong Sun,and Wen-Zhe Ho

1308–1309

Type IV Neonatal Early-Onset Group B StreptococcalDisease in a United States Hospital

Karen M. Puopolo and LawrenceC. Madoff

1360–1362

CASE REPORTSReport of the First Human Case of Caulobacter sp. Infection Ulrik S. Justesen, Hanne M. Holt,

Helle C. Thiesson, Jens Blom,Xiaohui C. Nielsen, Rimtas Dargis,Michael Kemp, and Jens J.Christensen

1366–1369

Zoonotic Vaccinia Virus Infection in Brazil: ClinicalDescription and Implications for Health Professionals

Giliane de Souza Trindade, BetaniaPaiva Drumond, Maria IsabelMaldonado Coelho Guedes, JulianaAlmeida Leite, Bruno EduardoFernandes Mota, Marco AntonioCampos, Flavio Guimaraes daFonseca, Maurıcio LacerdaNogueira, Zelia Ines PortelaLobato, Claudio AntonioBonjardim, Paulo Cesar PeregrinoFerreira, and Erna Geessien Kroon

1370–1372

Continued from preceding page

Continued on following page

LETTERS TO THE EDITORGluconate-Containing Intravenous Solutions: Another Causeof False-Positive Galactomannan Assay Reactivity

Ignace Surmont and WillemStockman

1373

The Operon Encoding SubAB, a Novel Cytotoxin, Is Presentin Shiga Toxin-Producing Escherichia coli Isolates from theUnited States

A. Khaitan, D. M. Jandhyala, C. M.Thorpe, J. M. Ritchie, and A. W.Paton

1374–1375

Nonspecific PCR Amplification of the 16S rRNA GeneSegment in Different Bacteria by Use of Primers Specific forCampylobacter, Arcobacter, and Helicobacter spp.

Amol D. Raut, Balu P. Kapadnis, R.Shashidhar, Jaynant R. Bandekar,Parag Vaishampayan, and Yogesh S.Shouche

1376–1377

Emergence of Non-Ceftriaxone-Susceptible Neisseriameningitidis in India

Letter: P. Nicolas. Reply: VikasManchanda and P. Bhalla

1378

Continued from preceding page