JOURNAL OF CLINICAL MICROBIOLOGY

Volume 46 July 2008 No. 7

BACTERIOLOGY

Rapid Screening for Carriage of Methicillin-ResistantStaphylococcus aureus by PCR and Associated Costs

Manuela Buhlmann, Katja Bogli-Stuber, Sara Droz, and KathrinMuhlemann

2151–2154

Accuracy of Phenotypic Methods for Identification ofStreptococcus pneumoniae Isolates Included in SurveillancePrograms

Sandra S. Richter, Kristopher P.Heilmann, Cassie L. Dohrn,Fathollah Riahi, Susan E.Beekmann, and Gary V. Doern

2184–2188

Evaluation of Two Commercial Systems for AutomatedProcessing, Reading, and Interpretation of Lyme BorreliosisWestern Blots

M. J. Binnicker, D. J. Jespersen,J. A. Harring, L. O. Rollins, S. C.Bryant, and E. M. Beito

2216–2221

PCR-Based Assay Using Occult Blood Detection Cards forDetection of Diarrheagenic Escherichia coli in Specimensfrom U.S. Travelers to Mexico with Acute Diarrhea

Kevin A. Grimes, Jamal A.Mohamed, Herbert L. DuPont,Ranjit S. Padda, Zhi-Dong Jiang,Jose Flores, Jaime Belkind-Gerson,Francisco G. Martinez-Sandoval,and Pablo C. Okhuysen

2227–2230

Capsular Polysaccharide Synthesis Regions in Klebsiellapneumoniae Serotype K57 and a New Capsular Serotype

Yi-Jiun Pan, Han-Chi Fang, Hui-Ching Yang, Tzu-Lung Lin, Pei-Fang Hsieh, Feng-Chiao Tsai, YoavKeynan, and Jin-Town Wang

2231–2240

Phylogenetic Backgrounds and Virulence Profiles of AtypicalEnteropathogenic Escherichia coli Strains from a Case-Control Study Using Multilocus Sequence Typing and DNAMicroarray Analysis

Jan Egil Afset, Endre Anderssen,Guillaume Bruant, Josee Harel,Lothar Wieler, and Kåre Bergh

2280–2290

Method To Detect Only Live Bacteria during PCRAmplification

Takashi Soejima, Ken-ichiro Iida,Tian Qin, Hiroaki Taniai, MasanoriSeki, and Shin-ichi Yoshida

2305–2313

Quadruplex Real-Time PCR Assay Using Allele-SpecificScorpion Primers for Detection of Mutations ConferringClarithromycin Resistance to Helicobacter pylori

Christophe Burucoa, MartineGarnier, Christine Silvain, andJean-Louis Fauchere

2320–2326

Direct Comparison of the BD Phoenix System with theMicroScan WalkAway System for Identification andAntimicrobial Susceptibility Testing of Enterobacteriaceaeand Nonfermentative Gram-Negative Organisms

J. W. Snyder, G. K. Munier, andC. L. Johnson

2327–2333

Optimization of a Laboratory-Developed Test UtilizingRoche Analyte-Specific Reagents for Detection ofStaphylococcus aureus, Methicillin-Resistant S. aureus, andVancomycin-Resistant Enterococcus Species

Maitry S. Mehta, Suzanne M. Paule,Donna M. Hacek, Richard B.Thomson, Karen L. Kaul, andLance R. Peterson

2377–2380

Impact of mecA Gene Testing and Intervention by InfectiousDisease Clinical Pharmacists on Time to OptimalAntimicrobial Therapy for Staphylococcus aureus Bacteremiaat a University Hospital

Peggy L. Carver, Shu-Wen Lin,Daryl D. DePestel, and Duane W.Newton

2381–2383

Rapid Identification of Penicillin and Macrolide ResistanceGenes and Simultaneous Quantification of Streptococcuspneumoniae in Purulent Sputum Samples by Use of a NovelReal-Time Multiplex PCR Assay

Kazuko Y. Fukushima, KatsunoriYanagihara, Yoichi Hirakata,Kazuyuki Sugahara, YoshitomoMorinaga, Shigeru Kohno, andShimeru Kamihira

2384–2388

Microbiological Characteristics, Presumptive Identification,and Antibiotic Susceptibilities of Staphylococcus lugdunensis

Thean Yen Tan, Siew Yong Ng,and Jie He

2393–2395

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Quadruplex PCR for Simultaneous Detection of Serotype,Biotype, Toxigenic Potential, and Central Regulating Factorof Vibrio cholerae

Hemant Kumar Khuntia, BibhutiBhusan Pal, and Guru PrasadaChhotray

2399–2401

Prevalence and Sequence Diversity of a Factor Required forActin-Based Motility in Natural Populations of BurkholderiaSpecies

Chayada Sitthidet, Joanne M.Stevens, Narisara Chantratita, BartJ. Currie, Sharon J. Peacock, SuneeKorbsrisate, and Mark P. Stevens

2418–2422

Isolation and Characterization of a Novel Francisella sp.from Human Cerebrospinal Fluid and Blood

Kiersten J. Kugeler, Paul S. Mead,Karin L. McGowan, Jon M.Burnham, Michael D. Hogarty,Eduardo Ruchelli, Kerry Pollard,Brigitte Husband, Caryn Conley,Tanya Rivera, Theodoros Kelesidis,Walter M. Lee, Walburga Mabey,Jonas M. Winchell, Heather L.Stang, J. Erin Staples, Linda J.Chalcraft, and Jeannine M. Petersen

2428–2431

Is the Incidence of Anaerobic Bacteremia Decreasing?Analysis of 114,000 Blood Cultures over a Ten-Year Period

Lukas Fenner, Andreas F. Widmer,Clarisse Straub, and Reno Frei

2432–2434

First Report of Isolation and Characterization ofAurantimonas altamirensis from Clinical Samples

Me-Linh Luong, Sadjia Bekal,Donald C. Vinh, DominiqueLauzon, Vicki Leung, Ghada N. Al-Rawahi, Betty Ng, Tamara Burdz,and Kathryn Bernard

2435–2437

Molecular Characterization of Staphylococcus aureusBloodstream Isolates Collected in a Dutch UniversityHospital between 1999 and 2006

Eric Nulens, Ellen E. Stobberingh,Helke van Dessel, Silvie Sebastian,Frank H. van Tiel, Patrick S.Beisser, and Ruud H. Deurenberg

2438–2441

Novel Chromogenic Medium for Detection of Vancomycin-Resistant Enterococcus faecium and Enterococcus faecalis

Gaëlle Cuzon, Thierry Naas, NicolasFortineau, and Patrice Nordmann

2442–2444

CHLAMYDIOLOGY AND RICKETTSIOLOGYIn Situ Detection of Anaplasma spp. by DNA Target-PrimedRolling-Circle Amplification of a Padlock Probe andIntracellular Colocalization with ImmunofluorescentlyLabeled Host Cell von Willebrand Factor

Heather L. Wamsley and AnthonyF. Barbet

2314–2319

MYCOBACTERIOLOGY AND AEROBICACTINOMYCETES

RDRio Mycobacterium tuberculosis Infection Is Associatedwith a Higher Frequency of Cavitary Pulmonary Disease

Luiz Claudio Oliveira Lazzarini,Silvana Miranda Spindola, HeejungBang, Andrea L. Gibson, ScottWeisenberg, Wania da SilvaCarvalho, Claudio Jose Augusto,Richard C. Huard, Afranio L.Kritski, and John L. Ho

2175–2183

Prevention of Drug Carryover Effects in Studies AssessingAntimycobacterial Efficacy of TMC207

Nacer Lounis, Tom Gevers, JokeVan Den Berg, Tom Verhaeghe,Rolf van Heeswijk, and KoenAndries

2212–2215

Multiplex Real-Time PCR Assay for Rapid Identification ofMycobacterium tuberculosis Complex Members to the SpeciesLevel

Benjamin A. Pinsky and NiazBanaei

2241–2246

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Biological and Molecular Characteristics of Mycobacteriumtuberculosis Clinical Isolates with Low-Level Resistance toIsoniazid in Japan

Chiyoji Abe, Ikuo Kobayashi,Satoshi Mitarai, Masako Wada,Yoshiko Kawabe, TetsuyaTakashima, Katsuhiro Suzuki, Li-Hwei Sng, Suxing Wang, Hla HlaHtay, and Hideo Ogata

2263–2268

Are Variable-Number Tandem Repeats Appropriate forGenotyping Mycobacterium leprae?

Marc Monot, Nadine Honore,Charlotte Baliere, Baohong Ji,Samba Sow, Patrick J. Brennan,and Stewart T. Cole

2291–2297

Tuberculosis Diagnosis and Multidrug Resistance Testing byDirect Sputum Culture in Selective Broth withoutDecontamination or Centrifugation

Louis Grandjean, Laura Martin,Robert H. Gilman, Teresa Valencia,Beatriz Herrera, Willi Quino, EricRamos, Maribel Rivero, RosarioMontoya, A. Roderick Escombe,David Coleman, Denis Mitchison,and Carlton A. Evans

2339–2344

MYCOLOGYClinical Evaluation of the Sensititre YeastOne ColorimetricAntifungal Panel for Antifungal Susceptibility Testing of theEchinocandins Anidulafungin, Caspofungin, and Micafungin

M. A. Pfaller, V. Chaturvedi, D. J.Diekema, M. A. Ghannoum, N. M.Holliday, S. B. Killian, C. C. Knapp,S. A. Messer, A. Miskov, and R.Ramani

2155–2159

Time to Blood Culture Positivity as a Marker for Catheter-Related Candidemia

Ronen Ben-Ami, MiriamWeinberger, Ruth Orni-Wasserlauff,David Schwartz, Avraham Itzhaki,Tzipora Lazarovitch, Edna Bash,Yuval Aharoni, Irina Moroz, andMichael Giladi

2222–2226

Real-Time PCR Method for Detection of Zygomycetes D. Jane Hata, Seanne P.Buckwalter, Bobbi S. Pritt, Glenn D.Roberts, and Nancy L. Wengenack

2353–2358

Detection of Caspofungin Resistance in Candida spp. byEtest

Marie Desnos-Ollivier, FrancoiseDromer, and Eric Dannaoui

2389–2392

Practical Method for Detection and Identification ofCandida, Aspergillus, and Scedosporium spp. by Use ofRolling-Circle Amplification

Xiaoyong Zhou, Fanrong Kong,Tania C. Sorrell, Hui Wang, YiqunDuan, and Sharon C. A. Chen

2423–2427

PARASITOLOGYMultiplex PCR and Oligonucleotide Microarray forDetection of Single-Nucleotide Polymorphisms Associatedwith Plasmodium falciparum Drug Resistance

Guo Qing Zhang, Ya Yi Guan, HaiHui Sheng, Bin Zheng, Song Wu,Hua Sheng Xiao, and Lin Hua Tang

2167–2174

Detection of Pathogenic Protozoa in the DiagnosticLaboratory: Result Reproducibility, Specimen Pooling, andCompetency Assessment

M. D. Libman, T. W. Gyorkos, E.Kokoskin, and J. D. MacLean

2200–2205

Identification of a Novel Babesia sp. from a Sable Antelope(Hippotragus niger Harris, 1838)

Marinda C. Oosthuizen, ErichZweygarth, Nicola E. Collins,Milana Troskie, and Banie L.Penzhorn

2247–2251

Classification of Cryptosporidium Species from Patients withSporadic Cryptosporidiosis by Use of Sequence-BasedMultilocus Analysis following Mutation Scanning

Aaron R. Jex, Aradhana Pangasa,Bronwyn E. Campbell, MargaretWhipp, Geoff Hogg, Martha I.Sinclair, Melita Stevens, and RobinB. Gasser

2252–2262

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Rapid, Novel, Specific, High-Throughput Assay forDiagnosis of Loa loa Infection

Peter D. Burbelo, RoshanRamanathan, Amy D. Klion,Michael J. Iadarola, and ThomasB. Nutman

2298–2304

LDBio-Toxo II Immunoglobulin G Western BlotConfirmatory Test for Anti-Toxoplasma Antibody Detection

Jacqueline Franck, Yves Jean-Francois Garin, and Henri Dumon

2334–2338

Genetic Diversity and Zoonotic Potential of Cryptosporidiumparvum Causing Foal Diarrhea

A. Grinberg, J. Learmonth, E.Kwan, W. Pomroy, N. LopezVillalobos, I. Gibson, and G.Widmer

2396–2398

VIROLOGYNovel Subgenotypes of Hepatitis B Virus Genotypes C andD in Papua, Indonesia

Maria Inge Lusida, Victor EkaNugrahaputra, Soetjipto, RetnoHandajani, Motoko Nagano-Fujii,Mikiko Sasayama, Takako Utsumi,and Hak Hotta

2160–2166

MDCK-SIAT1 Cells Show Improved Isolation Rates forRecent Human Influenza Viruses Compared to ConventionalMDCK Cells

Ding Yuan Oh, Ian G. Barr, JennyA. Mosse, and Karen L. Laurie

2189–2194

Comparison of the NucliSens easyMAG and QiagenBioRobot 9604 Nucleic Acid Extraction Systems forDetection of RNA and DNA Respiratory Viruses inNasopharyngeal Aspirate Samples

Kwok Hung Chan, Wing CheongYam, Chiu Mei Pang, Kit ManChan, Siu Yan Lam, Kam Fai Lo,Leo L. M. Poon, and J. S. MalikPeiris

2195–2199

Highly Sensitive Methods Based on Seminested Real-TimeReverse Transcription-PCR for Quantitation of HumanImmunodeficiency Virus Type 1 Unspliced and MultiplySpliced RNA and Proviral DNA

Alexander O. Pasternak, Karen W.Adema, Margreet Bakker, SuzanneJurriaans, Ben Berkhout, MarionCornelissen, and Vladimir V.Lukashov

2206–2211

Development of Multiplex PCR-Ligase Detection ReactionAssay for Detection of West Nile Virus

S. Rondini, M. R. Pingle, S. Das, R.Tesh, M. S. Rundell, J. Hom, S.Stramer, K. Turner, S. N.Rossmann, R. Lanciotti, E. G.Spier, J. Munoz-Jordan, D. Larone,E. Spitzer, F. Barany, and L. M.Golightly

2269–2279

Human Coronavirus NL63 and 229E Seroconversion inChildren

Ronald Dijkman, Maarten F.Jebbink, Nawal Bahia El Idrissi,Krzysztof Pyrc, Marcel A. Muller,Taco W. Kuijpers, Hans L. Zaaijer,and Lia van der Hoek

2368–2373

Comparison of Respiratory Virus Detection Rates forInfants and Toddlers by Use of Flocked Swabs, SalineAspirates, and Saline Aspirates Mixed in UniversalTransport Medium for Room Temperature Storage andShipping

Paul Walsh, Christina LimOvermyer, Kiemanh Pham, ScottMichaelson, Larisa Gofman, LisaDeSalvia, Ty Tran, Diana Gonzalez,James Pusavat, Melanie Feola,Kathryn T. Iacono, Eli Mordechai,and Martin E. Adelson

2374–2376

Molecular Characterization of Porcine Picobirnaviruses andDevelopment of a Specific Reverse Transcription-PCR Assay

Gabriela M. Carruyo, GuaniriMateu, Laura C. Martınez, Flor H.Pujol, Silvia V. Nates, FerdinandoLiprandi, and Juan E. Ludert

2402–2405

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Comparison between Pernasal Flocked Swabs andNasopharyngeal Aspirates for Detection of CommonRespiratory Viruses in Samples from Children

Afaf Abu-Diab, Maysa Azzeh, RaedGhneim, Riyad Ghneim, MadeleineZoughbi, Sultan Turkuman, NabeelRishmawi, Abed-El-Razeq Issa, IssaSiriani, Rula Dauodi, Randa Kattan,and Musa Y. Hindiyeh

2414–2417

EPIDEMIOLOGYMolecular Epidemiology of Methicillin-ResistantStaphylococcus aureus Bloodstream Isolates in Urban Detroit

Thea Chua, Carol L. Moore, MaryB. Perri, Susan M. Donabedian,William Masch, Dora Vager, SusanL. Davis, Kaitlin Lulek, BenjaminZimnicki, and Marcus J. Zervos

2345–2352

Epidemiology of Severe Streptococcus pyogenes Disease inEurope

Theresa L. Lamagni, JessicaDarenberg, Bogdan Luca-Harari,Tuula Siljander, AndroullaEfstratiou, Birgitta Henriques-Normark, Jaana Vuopio-Varkila,Anne Bouvet, Roberta Creti, KimEkelund, Maria Koliou, Ralf ReneReinert, Angeliki Stathi, LenkaStrakova, Vasilica Ungureanu, ClaesSchalen, the Strep-EURO StudyGroup, and Aftab Jasir

2359–2367

Epidemic of Genotype GII.2 Noroviruses during Spring 2004in Osaka City, Japan

Nobuhiro Iritani, Atsushi Kaida,Hideyuki Kubo, Niichiro Abe,Tsukasa Murakami, HarryVennema, Marion Koopmans,Naokazu Takeda, Hisashi Ogura,and Yoshiyuki Seto

2406–2409

Eight Years of Experience with Molecular Identification ofHuman Enteroviruses

Soile Blomqvist, Anja Paananen,Carita Savolainen-Kopra, TapaniHovi, and Merja Roivainen

2410–2413

CASE REPORTSTwo Cases of Keratitis and Corneal Ulcers Caused byBurkholderia gladioli

Franka Lestin, Robert Kraak, andAndreas Podbielski

2445–2449

Severe Thrombocytopenia Associated with Acute Hepatitis EVirus Infection

P. Colson, E. Payraudeau, C.Leonnet, S. De Montigny, L.Villeneuve, A. Motte, and C.Tamalet

2450–2452

Difficulties in Diagnosing Group O HumanImmunodeficiency Virus Type 1 Acute Primary Infection

Cecile Henquell, Christine Jacomet,Odile Antoniotti, Abdelhak Chaib,Christel Regagnon, Sylvie Brunet,Helene Peigue-Lafeuille, andFrancis Barin

2453–2456

DNA Typing of Cytological Samples for RetrospectiveIdentification of an Early Case of Panton-ValentineLeucocidin-Positive, Community-Associated Methicillin-Resistant Staphylococcus aureus Pneumonia

P. L. Ho, M. P. Wong, Eileen L.Lai, K. H. Chan, and Susan S. Chiu

2457–2458

Mycobacterium haemophilum Epididymal Abscess in a RenalTransplant Patient

Marina Keller, Anton Mak, LouiseThibert, Pierre Rene, and Marina B.Klein

2459–2460

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FAST-TRACK COMMUNICATIONFirst Identification and Molecular Characterization ofLymphotropic Polyomavirus in Peripheral Blood fromPatients with Leukoencephalopathies

Serena Delbue, Sara Tremolada,Emanuela Branchetti, FrancescaElia, Elisa Gualco, EnricoMarchioni, Renato Maserati, andPasquale Ferrante

2461–2462

LETTERS TO THE EDITOREffect of Epstein-Barr Virus Sequence Variation onPerformance of the Roche Real-Time LightCycler PCRAssay

Tao Feng, Fei Ye, Josephine Wu,Shiquan Jiang, Jennifer Coolbaugh,Benjamin Shneider, Betsy Herold,Sukru Emre, and David Y. Zhang

2463–2465

vanC Gene-Related Intrinsic Teicoplanin ResistanceDetected in Enterococcus casseliflavus and E. gallinarumStrains by the BD Phoenix Automated Microbiology System

Mustafa Berktas, Gorkem Yaman,and Oznur Ozturk

2466

How Valid Is Single-Colony Isolation for Surveillance ofStreptococcus pneumoniae Carriage?

B. M. Charalambous, Ndekya M.Oriyo, and S. H. Gillespie

2467–2468

Nocardia cyriacigeorgica—an Established Rather than anEmerging Pathogen

Letter: Frank G. Witebsky, PatriciaS. Conville, Richard J. Wallace, Jr.,Barbara A. Brown-Elliott. Reply:Richard C. Huard, RobertSchlaberg, and Phyllis Della-Latta

2469–2470

Clinical Rationale for Treatment of Endocarditis Caused byMethicillin-Susceptible Staphylococcus aureus DevelopingNonsusceptibility to Daptomycin

Letter: Zukile Gqada, Ben Marais,Elizabeth Wasserman, ColleenBamford, Heidi Orth. Reply: GeorgeSakoulas, Warren Rose, MichaelJ. Rybak, Satish Pillai, Jeff Alder,Robert C. Moellering, Jr., andGeorge M. Eliopoulos

2471–2472

ERRATAHospital-Based Evaluation of Two Rapid HumanImmunodeficiency Virus Antibody Screening Tests

Rajesh Kannangai, SandeepRamalingam, Pradeep K. Selvaraj,Kannan Damodharan, and GopalanSridharan

2473

Real-Time PCR Assays of Single-Nucleotide PolymorphismsDefining the Major Brucella Clades

Jeffrey T. Foster, Richard T.Okinaka, Rita Svensson, KathrynShaw, Barun K. De, Richard A.Robison, William S. Probert, LeoJ. Kenefic, William D. Brown, andPaul Keim

2474

Timing of Specimen Collection for Blood Cultures fromFebrile Patients with Bacteremia

Stefan Riedel, Paul Bourbeau,Brandi Swartz, Steven Brecher,Karen C. Carroll, Paul D. Stamper,W. Michael Dunne, TimothyMcCardle, Nathan Walk, KristinFiebelkorn, David Sewell, Sandra S.Richter, Susan Beekmann, and GaryV. Doern

2475

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