JOURNAL OF BACTERIOLOGYVOLUME 108 * NUMBER 3 * DECEMBER 1971

. EDITORIAL BOARD

L. LEON CAMPBELL, Editor-in-Chief(1975)University ofIllinois, Urbana

R. L. ANDERSON, Editor (1975) ROY CURTISS III, Editor (1974)Michigan State University, East Lansing The University of Tennessee, Oak Ridge

S. G. BRADLEY, Editor (1974) ALLEN G. MARR, Editor (1973)Virginia Commonwealth University, Richmond University of California, Davis

H. E. UMBARGER, Editor (1976)Purdue University, Lafayette, Ind.

Martin Alexander (1972)W. Lane Barksdale (1972)M. E. Bayer (1972)Claire M. Berg (1973)Robert W. Bernlohr (1973)Allan Campbell (1972)A. J. Clark (1972)Royston C. Clowes (1972)V. W. Cochrane (1972)Stanley N. Cohen (1973)S. F. Conti (1972)A. L. Demain (1972)John A. DeMoss (1972)Walter J. Dobrogosz (1973)Martin Dworkin (1973)Alan D. Elbein (1973)Hlerman Friedman (1972)A. T. Ganesan (1972)Howard Goldrine (1972)Joseph S. Gots (1972)Harlyn 0. Halvorson (1972)F. M. Harold (1973)

Leonard Hayflick (1972)George Hegeman (1971)Charles E. Helmstetter (1972)Joseph T. Holden (1972)John G. Holt (1972)Stanley C. Holt (1971)John Ingraham (1973)John H. Law (1971)Hillel S. Levinson (1972)W. R. Lockhart (1972)Donald G. Lundgren (1971)Manley Mandel (1972)Paul Margolin (1972)Alvin Markovitz (1973)Julius Marmur (1971)Robert G. Martin (1971)M. L. Morse (1972)Richard P. Novick (1973)Leo Parks (1973)fl. J. Phaff (1972)Allen T. Phillips (1973)Jack Preiss (1971)Charles C. Remsen III (1973)

William S. Reznikoff (1973)H. V. Rickenberg (1973)Antonio H. Romano (1973)John Roth (1973)M. R. J. Salton (1972)Moselio Schaechter (1973)David Schiessinger (1971)Simon Silver (1973)W. R. Sistrom (1972)Lucile Smith (1972)Bernard S. Strauss (1972)Noboru Sueoka (1972)Paul S. Sypherd (1971)A. L. Taylor (1971)H. E. Umbarger (1973)Arthur Weissbach (1971)N. E. Welker (1973)R. W. Wheat (1972)Helen R. Whiteley (1972)Meyer J. Wolin (1973)Barbara E. Wright (1971)Frank E. Young (1973)Stanley A. Zahler (1971)

ROBERT A. DAY, Managing Editor, 1913 I St., N.W., Washington, D.C. 20006

EX OFFICIOMORRIS F. SHAFFER, President (1971-1972)

DONALD E. SHAY, Secretary

The Journal of Bacteriology, a publication of the American Society forMicrobiology, 1913 1 St., N.W., Washington, D.C. 20006, is devoted to

the advancement and dissemination of fundamental knowledge concerningbacteria and other microorganisms. The Journal is published monthly, andthe twelve numbers are divided into four volumes per year. The subscrip-tion price is $52 (Postal Union Countries, $54; other foreign, $55) per

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general editorial matters should be directed to the ASM Publications

Office, 1913 1 St., N.W., Washington, D.C. 20006. Correspondence from

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R. G. E. MURRAY, Vice-President (1971-1972)T. J. CARSKI, Treasurer

changes of address, incorrect journals, etc., should be directed to the

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lished monthly by the ASM at 428 E. Preston St., Baltimore, Md. 21202.

Second class nostage paid at Baltimore, Md. 21202, and at additionalmailing offices.Made in the United States of America.Copyright (D 1971, American Society for MicrobiologyAll Rights Reserved.

AUTHOR INDEXAbbondandolo, Angelo, 959Allen, Richard D., 1380Allen, Robert G., 1296Allison, David P., 1390Apirion, David, 1322Atkinson, Daniel E., 1072

Baron, L. S., 1357Baumann, Linda, 1380Baumann, Paul, 1380Behforouz, Nancy C., 1129Billen, Daniel, 1250Bock, August, 1008Brandriss, Marjorie C., 1431Breitman, T. R., 1168Bridges, Bryn A., 1422Brodie, Arnold F., 1017Brody, Stuart, 1310Buckel, Peter, 1008

Calvo, J. M., 1431Carreira, Laura B., 1250Carter, B. L. A., 1045Cavari, Ben-Zion, 1017Chapman, Astrid G., 1072Cook, Thomas M., 1026

Daneo-Moore, Lolita, 980DasGupta, Bibhuti R., 1051Datta, Naomi, 1244Dayan, J., 1174Dempsey, Walter B., 1001Dieguez, Eugenia, 1338Doelle, Horst, W., 1284, 1290

Eisenberg, Max A., 1135Eisenberg, R. C., 964Englund, Per, 1210Eriksson-Grennberg, Kerstin G.,

1210

Fall, Lana, 1072Fankhauser, David B., 1427Filip, Camille, 1384Finkleman, A., 1168Forsberg, C. W., 1235Fradkin, Judith E., 1277Fraenkel, D. G., 1277Freese, Ernst, 1129

Gallant, J., 1424Gest, Howard, 1122Goldman, Peter, 996Gormus, B. J., 1304Griffith, Mary E., 1154Guardiola, John, 1034Guirard, Beverly M., 1318Gustafson, Gary, 1329

Hadden, Charles T., 1250Hahn, Fred E., 1026Halvorson, H. 0., 1045Hampton, Michael L., 1129Harada, B., 1424Hartman, Philip E., 1427Hattori, Hiroshi, 1412Hayasaka, Steven, 1141Hedges, R. W., 1244Hegeman, George D., 973, 1270Heslot, Henri, 959

Iaccarino, Maurizio, 1034lida, Kyoko, 1402Iwashima, Akio, 1419

Johnson, E. M., 1357Johnson, Marvin J., 1107

Kalra, Vijay K., 1017Kaplan, Ruth, 1147Katayama, Yutaka, 1412Kistler, W. S., 1224Klein, D. A., 1141Klemme, Brigitte, 1122Klemme, Jobst-Heinrich, 1122Koike, Masaatsu, 1402Krymkiewicz, Norberto, 1338

Lambert, Micheline, 959Lambowitz, Alan M., 1087Leavitt, R. W., 1357Lennette, Evelyne T., 1322Levin, Richard A., 992Lin, E. C. C., 1224Lubitz, Werner, 1008

McCormick, Neil G., 1129Mandel, M., 1380Matsuura, Atsuko, 1419Middleton, R. B., 1161Mojica-a, T., 1161

Nordstrom, Kurt, 1210Nose, Yoshitsugu, 1419Nyc, Joseph F., 1310

Olson, G. E., 1357Ono, Yoshie, 1065

Pan, Pauline H. C., 1416Park, James T., 1154Peppercorn, Mark A., 996Pope, Leodocia, 1384

Rabinovitz, M., 1168Rekarte, Usua D., 1338Richmond, M. H., 1244Rode, L. J., 1384Rogers, H. J., 1235Rogers, Lynne A., 1J 12Rosenberg, S. L., 1257, 1270Rosenblum, E. D., 1192, 1200Rosness, Per A., 1329Roth, George S., 980Rubin, Sally J., 1192, 1200

Schwalb, Marvin N., 1205Sebastian, J., 1045Sedgwick, Steven G., 1422Shafiq, Nawal A., 1296Shaw, Elizabeth J., 1244Silverstein, Saul J., 1250Simon, M. I., 1366Slayman, Carolyn W., 1087Smith, Louis DS., 1384Smith, Paul F., 986Snell, Esmond E., 1318Soreq, Hermona, 1147Sprinson, D. B., 1174Stevenson, Ian, 1154Stoner, Gerald L., 1135Stubbs, E. Ann, 1181Stubbs, John D., 1181Suganuma, Atsushi, 1412Sybesma, C., 1348Sykes, R. B., 1244

Tanaka, Nobumasa, 1107Truper, Hans G., 1112Tucker, Anne N., 1058

Uffen, R. L., 1348

Van Alstyne, D., 1366

Walker, James R., 1296Weimberg, Ralph, 1097Weyer, Anne, 959Wheat, R. W., 1304White, David C., 1058, 1065Wilkins, A., 1424Wohlhieter, J. A., 1357Wolfe, Alan D., 1026Wolfe, R. S., 1348Wright, Barbara E., 1329

Yajko, David M., 973

Zwaig, Noe, 1338

Acknowledgment

The following have served as invited specialtheir help is greatly appreciated.

reviewers for the Journal during 1971, and

Adolph AbramsDinah AbramsJ. N. AdamsE. A. AdelbergH. I. AdlerJulius AdlerS. J. AjlJ. M. AkagiM. I. H. AleemM. B. AllenR. A. AltenbergBruce AmesGiovanna F. AmesD. L. AndersonE. S. AndersonD. ApirionF. B. ArmstrongD. E. AtkinsonAnnette BaichE. BalbinderJ. N. BaldwinS. D. BarbourH. A. BarkerS. Bartnicki-GarciaB. BeamanJ. R. BeckwithW. BeersWilliam BelserE. BenjaminA. A. BensonM. S. BergdollR. S. BerkA. W. BernheimerL. L. BieberD. BillenJ. A. BoeziJ. BonnerR. P. BoyceH. BoyerC. BradbeerR. W. BreidenbachT. D. BrockH. BruckmanS. BrodyN. BrotG. H. BrownellM. P. BryantR. H. BurrisR. U. ByerrumJ. M. CalvoJ. A. CarbonMichael CashelP. L. CarlB. C. CarltonB. W. CatlinG. B. ChapmanS. ChaykinD. J. ClarkW. A. ClydeS. N. CohenS. S. CohenR. M. ColePhilip ColemanE. B. CollinsE. E. ConnE. H. Cota-Robles

F. L. CraneR. S. CriddleD. J. CummingsS. DagleyA. S. DahmsH. DaltonP. DattaB. 0. DavisR. H. DavisW. C. Deal, Jr.B. T. De CiccoR. H. DeibelR. D. DeMossD. T. DenhardtAlma DietzJ. C. DittmoorR. N. DoetschR. H. DoiMichael DoudoroffH. C. DouglasC. E. DowellJ. W. DrakeJ. D. DuerksenR. G. EagonG. L. EdlinJ. EignerM. A. EisenbergA. EisenstarkH. L. EnnisJ. C. EnsignW. EpsteinM. S. EspositoD. H. EzelielD. P. FanT. W. FearyD. S. FeingoldP. FilnerG. R. FinkC. F. FoxD. G. FraenkelJ. FralickL. H. FrankN. FranklinE. FreeseA. FrenkelJ. R. FrescoM. FreundlichE. A. FreundtLorraine FreidmannD. FriefelderT. T. FukuyamaA. J. FulcoC. R. FullerC. FurlongF. G. GaertnerJ. GallantJ. GallyE. GarberJ. L. GaylorH. GestR. J. GibbonsM. N. GilmourC. GilvargI. J. GoldsteinHarry GooderL. Gorini

M. A. GordonJ. GormanG. W. GouldG. R. GreenbergJ. W. GreenwaltA. F. GronlundE. GrulaW. R. GuildBeverly GuirardL. P. HagerP. B. HamiltonR. HammerstedtD. C. HanawaltM. W. HaneA. P. Harrison, Jr.P. A. HartmanP. E. HartmanL. H. HartwellR. J. HarveyC. HeidelbergerD. R. HelinskiL. A. HeppelR. K. HermanC. L. HershbergerPeter HirschJ. A. HochL. I. HochsteinP. D. HoeprichR. HotchkissH. S. HsuJ. S. HubbardF. M. HuennekensR. E. HungateM. InouyeN. JacobsAnn JacobsonE. M. JohnsonM. J. JohnsonR. JohnsonBeth JonesH. R. KabackT. KaneshiroSam KaplanM. J. KarabatsosW. KeefeN. E. KelkerE. S. KempnerD. E. KennellC. KenyonH. T. KnightonA. L. KochR. A. KolstadJ. KoniskyH. L. KornbergL. 0. KrampitzE. G. KrebsH. E. KubitschekC. G. KurlandS. LacksJ. 0. Lampen0. E. Landman.K. G. LarkJune LascallesE. R. LeadbetterH. LechevalierM. P. Lechevalier

P. LederS. LederbergT. J. LeonardT. G. LessieA. P. LevinJ. C. LewisS. L. Liaaen-JensenE. C. C. LinJack LondonW. R. Loomis, Jr.K. B. LowS. E. LuriaW. K. MaasB. MagasanikG. P. ManireDavid MardonW. H. MatchettPeter MazurA. MeisterN. H. MendelsonR. L. MetzenbergL. MindichS. MitraK. J. MontyL. E. MortensonJ. W. MoulderR. G. E. MurrayB. J. McCarthyE. McFallF. C. NeidhardtE. W. NesterJ. NeuhardF. NeuhausA. NewtonW. J. NickersonD. J. NiederpruemM. NomuraJ. H. NordinG. A. O'DonovanEvelyn OginskyM. OishiW. M. O'LearyZ. J. OrdalL. N. OrnstonM. J. OsbornR. E. PachaP. R. PainterN. J. PalleroniD. PappagianisA. B. Pardee

D. ParetskyE. C. PaulingW. J. PayneH. D. Peck, Jr.F. 0. PerkinsR. PerlmanP. PhibbsA. W. PhillipsLeo PineL. PizerB. PlappJ. R. PostgateD. PrattJ. D. PunchJ. R. QuayleH. RappaportE. T. ReeseRoy RepaskeM. ResnickEdgar RibiC. C. RichardsonMonica RileyS. C. RittenbergP. C. RobertsonW. R. RomigK. J. RoozenA. H. RoseE. D. RosenblumE. H. RunyonW. D. RuppR. L. RussellC. B. SabistonL. E. SacksV. L. SapicoD. C. SavageH. G. SchlegelC. SchnaitmanI. SegelJane SetlowR. B. SetlowH. J. ShadomyS. K. ShapiroW. V. ShawR. SheehyG. D. ShockmanL. ShugartR. SilverJohn SlackH. D. SladeR. Slepecky

K. C. SmithPaul SmithR. S. SnowD. R. StadlerM. P. StarrB. A. D. StockerR. L. StorckJ. L. StromingerF. W. StudierM. P. StulbergJ. H. StuyC. H. SuelterJ. C. SuitM. SussmanC. C. SweeleyD. W. TempestR. TennantD. J. TipperN. E. TolbertA. TomaszG. M. TomkinsA. TorrianiS. B. ToveM. F. UtterR. P. VagelosR. ValentineJ. E. VarnerW. VishniacWes VolkE. W. Voss, Jr.J. T. WachsmanJ. R. WalkerL. G. WayneR. WeimbergE. WeissD. W. S. WestlakeM. L. WheelisD. C. WhiteJ. R. WhittakerH. WiesmeyerR. C. WilhelmR. P. WilliamsJ. B. WilsonP. W. WilsonC. R. WoeseJ. A. WohlhieterR. S. WolfeD. WoodwardCharles YanofskyG. Zubay

JOURNAL OF BACTERIOLOGYINSTRUCTIONS TO AUTHORS

Submit manuscripts in duplicate (original and onecarbon) to ASM Publications Office, 1913 I St., N.W.,Washington, D.C. 20006.

General policy. Any manuscript submitted must bea report of unpublished original research, which is notbeing considered for publication elsewhere. Each manu-script should present the results of an independent, co-hesive study; "series" papers are discouraged. A manu-script accepted and published by the Journal must notbe published again in any form without the consent ofASM.

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cation in the Journal. Most institutions and grantingagencies in the United States permit the payment ofpublication charges as a part of their general researchsupport. It is recognized, however, that such funds arenot always available to laboratories outside the UnitedStates. In such cases, the cost of publication will beborne by the American Society for Microbiology.The "editorial style" of the Journal essentially fol-

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INDEX TO DATE OF ISSUE

Month Date of Issue Pages

October 11 October 1971 1-620November 4 November 1971 621-958December 8 December 1971 959-1434

AUTHOR INDEX

VOLUME 108

Abbondandolo, Angelo, 959Adhya, Sankar, 621Albro, Phillip W., 213Allen, Richard D., 1380Allen, Robert G., 1296Allison, David P., 1390Ames, Bruce N., 359, 410Apirion, David, 1322Argoudelis, Aleyander D., 459Aronson, A. I., 571Atherly, Alan G., 627Atkinson, Daniel E., 1072

Baptist, James N., 799Barile, Michael F., 439Barksdale, L., 941Baron, L. S., 1357Bassel, John, 179, 609Batzing, B. L., 592Baumann, Linda, 1380Baumann, Paul, 1380Beaman, B. L., 941Beaman, T. Cabrera, 868Beckwith, J. R., 5Beebe, James L., 612Behal, Francis J., 809Behforouz, Nancy C., 1129Bhattacharjee, Jnanendra K., 715Billen, Daniel, 1250Bock, August, 1008Borek, Ernest, 446Bott, Kenneth F., 720Brandriss, Marjorie C., 1431Breitman, T. R., 1168Bridges, Bryn A., 944, 1422Brock, Thomas D., 227Brodie, Arnold F., 1017Brody, Stuart, 1310Brown, Donna F., 705Brown, K. D., 386Brown, Karen K., 82Buckel, Peter, 1008Bulla, Lee A., Jr., 828Bull, A. T., 309

Calvo, J. M., 95, 1431Carreira, Laura B., 1250Carski, Theodore R., 439Carter, B. L. A., 309, 1045Castric, Peter A., 132Cavari, Ben-Zion, 1017Chang, George W., 410Chapman, Astrid G., 1072Chatterjee, Anadi N., 584Clark, Joseph D., 74Claus, G. W., 592Coats, John H., 459

Cole, K. W., 902Conn, Eric E., 132Cook, Thomas M., 320, 1026Cooney, J. J., 777Corner, Thomas R., 482, 492, 501Cozzarelli, Nicholas R., 364Crawford, Irving P., 248, 431

Dale, Beverly A., 586Daneo-Moore, Lolita, 980Daniel, Wendell A., 526D'Aoust, J. Y., 916DasGupta, Bibhuti R., 1051Datta, Naomi, 1244Dave, Bhalchandra A., 166Davidson, H., 400Dayan, J., 1174DelGiudice, Richard A., 439DeMoss, J. A., 854Dempsey, Walter B., 415, 1001Dieguez, Eugenia, 1338Dimmitt, K., 282Doelle, Horst, W., 1284, 1290Dooley, Douglas C., 668Drabble, Willham T., 159Drucker, D. B., 515

Eisenberg, Max A., 1135Eisenberg, R. C., 964Elliker, P. R., 599, 604Ellison, Jane S., 526Enatsu, Toshio, 431Englund, Per, 1210Ensign, J. C., 751, 757Epstein, Wolfgang, 639Eriksson-Grennberg, Kerstin G.,

1210

Fall, Lana, 1072Fankhauser, David B., 1427Filip, Camille, 1384Finkleman, A., 1168Fitz-James, P. C., 571Foerster, Harold F., 817Forsberg, C. W., 1235Fox, Maurice S., 680Fradkin, Judith E., 1277Fraenkel, D. G., 1277Fraker, Pamela J., 465Freedberg, W. B., 137Freese, Ernst, 1129Freundlich, Martin, 951Friedman, S. Marvin, 589

Gaertner, F. H., 902Gallant, J., 1424Galun, Esra, 938

i

Garrett, M. Kerry, 59Gass, Kenneth B., 364Gates, F., 742Gaudy, Elizabeth T., 82Gerhardt, Philipp, 482, 492, 501,

868Gest, Howard, 1122Ghosh, B. K., 928Gibson, Jane, 954Glaser, J. H., 854Goldman, Peter, 996Goldstein, T., 742Gormus, B. J., 1304Goulian, Mehran, 364Granato, Paul A., 804Griebel, R. J., 782Griffith, Mary E., 1154Guardiola, John, 1034Guespin-Michel, J. F., 241Guirard, Beverly M., 359, 1318Gustafson, Gary, 1329

Hadden, Charles T., 668, 1250Hahn, Fred E., 320, 1026Hall, M. J., 314Halvorson, H. O., 1045Hamilton, P. B., 596Hampton, Michael L., 1129Hanson, Sally, 765Harada, B., 1424Hartman, Philip E., 1427Hatten, Betty A., 535Hattori, Hiroshi, 1412Hayasaka, Steven, 1141Hedges, R. W., 1244Hefernan, Laurel, 1Hegeman, George D., 973, 1270Hendry, D. A., 765Heslot, Henri, 959Hill, Thomas C., 364Hiragi, Yuzuru, 579Hirota, Y., 375Hirschberg, Rona, 751, 757Hoar, David I., 582Hoare, Derek S., 733Hoeksema, Walter D., 154Huang, Shyi Yi, 535Huzyk, Lydia, 74Hyatt, Mildred T., 111

laccarino, Maurizio, 1034Iida, Kyoko, 1402Im, S. W. K., 400Ippen, K., 5Ito, Toichi, 191Iwashima, Akio, 1419Iyobe, Shizuko, 946

AUTHOR INDEX

Jackson, Robert W., 804, 844Jacob, F., 375Jacoby, George A., 645Jenkins, Philip G., 159Jockusch, Brigitte M., 705Johnson, E. M., 1357Johnson, Marvin J., 1107Joswick H. L., 482, 492, 501

Kadota, Hajime, 579Kalra, Vijay K., 1017Kaplan, Ruth, 1147Kaplan, Samuel, 465Katayama, Yutaka, 1412Katz, Dvorah, 184Kelly, Beatrice, 695Kennedy, C. K., 10Keudell, K. C., 175Kim, Byung S., 639Kim, K. S., 941Kistler, W. S., 137, 1224Klein, D. A., 1141Klemme, Brigitte, 1122Klemme, Jobst-Heinrich, 1122Kloos, Wesley E., 353Koike, Masaatsu, 1402Krymkiewicz, Norberto, 1338Kulczyk, Sandra R., 196Kurtz, Mark, 715Kushner, D. J., 916

Lambert, Micheline, 959Lambowitz, Alan M., 1087Lampen, J. O., 928Lancini, G. C., 737Lapidus, I. Richard, 607Lawrence, Paul J., 662Leavitt, R. W., 1357Lemcke, Ruth M., 439Lennette, Evelyne T., 1322Lesko, Stephen A., Jr., 202Lev, M., 175Levin, Richard A., 992Levinson, Hillel S., 111Levy, Stuart B., 300Lin, E. C. C., 137, 375, 1224Lipman, Muriel B., 508London, Jack, 196Longo, D., 601Low, B., 742Lubitz, Werner, 1008Lugtenberg, E. J. J., 20Lundgren, D. G., 328, 334, 343

McCormick, Neil G., 1129McFeters, G. A., 599McGinnis, E., 254MacGregor, C. H., 564Machtiger, Neal A., 948Maher, Veronica M., 202Maley, Frank, 145Mandel, Manley, 799, 1380Matsuura, Atsuko, 1419Mattern, Carl F. T., 526Merrick, J. M., 782

Meyer, Eleanor Y., 196Middleton, R. B., 1161Milford, A. F., 175Miller, A., III, 604Miovic, Margaret L., 954Mitsuhashi, Susumu, 946Mojica-a, T., 1161Monier, Roger, 105Morita, Richard Y., 835Morrison, D. A., 38Morrison, Susan J., 353Morse, Helvise G., 690Morse, M. L., 690Mortimer, Robert, 179, 609Mortlock, R. P., 287, 293Mosmann, T. R., 765Murashima, Ryuzo, 191

Nanninga, N., 474Nester, Eugene W., 586, 668Nordstrom, Kurt, 1210Normark, Staffan, 45, 51Nose, Yoshitsugu, 1419Nyc, Joseph F., 1310

O'Leary, William M., 948Oliver, Eugene J., 287, 293Olson, G. E., 1357Olszowy, Joyce, 122Ono, Yoshie, 1065

Page, W. J., 276Pan, Pauline H. C., 1416Park, James T., 874, 1154Parker, Donald J., 770Patching, J. W., 451Paul, Kala L., 835Penberthy, William K., 690Peng, Willow, 951Peppercorn, Mark A., 996Piechowska, Miroslawa, 680Pirt, S. J., 309Pittard, A. J., 790Pittard, J., 400Pope, Leodocia, 1384Proby, C. M., 777

Rabinovitz, M., 1168Ratledge, C., 314Ray, Paul H., 227Reeves, Henry C., 824Reiner, Albey M., 89Rekarte, Usua D., 1338Reusser, Fritz, 30Richmond, M. H., 1244Riley, P. S., 809Rode, L. J., 1384Rogers, H. J., 1235Rogers, Lynne A., 1112Rogolsky, Marvin, 662Ron, Eliora Z., 263Rose, A. H., 451Rose, John K., 615Rosenberg, S. L., 1257, 1270Rosenberger, R. F., 184

Rosenblum, E. D., 1190, 1200Rosner, Arie, 760Rosness, Per A., 1329Roth, George S., 980Roth, John R., 410Rowe, John J., 824Rowley, B. I., 309Rubin, Sally, J., 1192, 1200Rupp, W. Dean, 508Rusch, Harold P., 705Russell, R. R. B., 790Rutherford, C. L., 269

Sabet, Sohair Farid, 422St. Julian, Grant, 828Salanitro, Joseph P., 885, 893Salton, M. R. J., 941Sandine, W. E., 599, 604Sargent, Malcolm, 59Scarborough, Gene A., 446Scherrer, Ren6 868Schlessinger, D., 601Schnaitman, Carl A., 422, 545,

553, 564Schoenhard, Delbert E., 154Schulze, M. L., 535Schwalb, Marvin N., 1205Schwartz, Maxime, 621Sebastian, J., 1045Sedgwick, Steven G., 1422Shafiq, Nawal A., 1296Shapiro, J. A., 5Shaw, Charles R., 799Shaw, D. R. D., 584Shaw, Elizabeth J., 1244Shelley, Gail, 596Shimoyama, Makoto, 191Short, Steven A., 219Silvernale, J. N., 482, 492, 501Silverstein, Saul J., 1250Silvestri, L. G., 737Simon, M. I., 282, 1366Singer, Judith, 1Sinha, Asru K., 715Slayman, Carolyn W., 1087Smith, Louis DS., 1384Smith, Paul F., 986Snell, Esmond E., 359, 1318Soll, D., 742Somerville, R. L., 386Soreq, Hermona, 1147Sperl, George T., 733Sprinson, D. B., 1174Staley, J. T., 236Stevenson, Ian, 1154Stieglitz, B., 95Stock, J. J., 276Stoner, Gerald L., 1135Straat, Patricia A., 202Strauss, Bernard S., 364Strnad, Bruce C., 910Stubbs, E. Ann, 1181Stubbs, John D., 1181Suchanek, Martha C., 627Suganuma, Atsushi, 1412

* k J. BACTERIOL.

AUTHOR INDEX

Sulkin, S. Edward, 535Sunshine, Melvin G., 695Sussman, Alfred S., 59Switzer, R. L., 122Sybesma, C., 1348Sykes, R. B., 1244Sypherd, Paul S., 910

Tabita, Robert, 328, 334, 343Tanaka, Nobumasa, 1107Tanigawa, Yoshinori, 191Thi Hanh, Vo, 662Tochikubo, Kunio, 652Tomoda, Tsunesuke, 191Tornabene, T. G., 353Trela, John, 951Trimble, R. B., 145Truper, Hans G., 1112Tsay, San-San, 82Ts'o, Paul 0. P., 202Tucker, Anne N., 1058Tully, Joseph G., 439

Ueda, Iwao, 191Uffen, R. L., 1348

Umbreit, W. W., 612Umezawa, Hamao, 946

Van Alstyne, D., 1366van Bellegem, T. H. M., 20Vanderslice, Rebecca W., 849Van Ert, M., 236Van Gool, A. P., 474van Schijndel-Van Dam, Arna, 20Vapnek, Daniel, 508Varricchio, Frederick, 105Vaughn, Reese H., 166Veerkamp, J. H., 861Venkov, P., 601

Walker, James R., 1296Warfel, Jean, 609Wegener, Warner S., 885, 893Weimberg, Ralph, 1097Welch, G. R., 902Werth, Jean M., 844Westling, Britta, 45Weyer, Anne, 959Wheat, R. W., 1304White, David C., 219, 227, 1058,

1065

White, Mary N., 122White, R. J., 737Whittaker, D. K., 515Wilcox, Gary, 1Wilkins, A., 1424Williams, L. S., 254Wohlhieter, J. A., 1357Wolfe, Alan D., 320, 1026Wolfe, R. S., 1348Wong, Rosemary S. L., 446Wood, Harland G., 770Woods, D. R., 765Woods, Robin A., 69Wouters, J. T. M., 928Wright, Barbara E., 269, 1329Wu, Teresa C. M., 874Wu, Tsai-feng, 770

Yagil, Ezra, 760Yajko, David M., 973Yanofsky, Charles, 248, 615Yegian, Charles D., 849Yu, Shih-an, 59

Zwaig, Noe, 1338

* *VOL. 108, 1971

SUBJECT INDEX

VOLUME 108

Acetate synthesis-from CO2, 770Acetobacter suboxydans-biphasic growth of, 592-glycerol oxidation bv, 592O-Acetyl groups-as component of phage receptor, 584Acetyl phosphate-conversion of pyruvate to, 1107O-Acetylserine-role in ,6-CNA synthesis, 132O-Acetylserine sulfhydrylase-role in ,8-DNA synthesis, 132Acid phosphatase-in S. mellis, 1097-sequence of events for recovery, 1097Acriflavine resistance-in S. marcescens, 765Acrylamide gel electrophoresis-use in B. subtilis studies, 720Actin-in P. polycephalum nucleus, 705Activation of botulinum toxin, 1051Adenine aminohydrolase-in S. pombe, 959Adenine nucleotide concentrations-various organisms and tissues, 1072Adenosine triphosphate-inhibition of ribonuclease II, 601S-Adenosyl-L-methionine

7,8-diaminopelargonic acid from, 1135Adenylate energy charge-in E. coli, 1072Adenylyl sulfate reductase-from sulfate-reducing bacteria, 1112-from thiobacilli, 1112-from T. roseopersicina, 1112Aerobacter aerogenes-enzyme activities in, 287-growth on D-arabinose, 287-metabolism of D-arabinose, 293-mutants of, 287, 293Agrobacterium tumefaciens-tumor induction by, 973Alanyl-tRNAt synthease-E. coli, 1008Aliphatic hydrocarbons-genetic studies with, 353-in Micrococcacae, 353Alkaline phosphatase activity-distribution of sites, 928-in B. subtilis, 928Alkaline sucrose gradient sedimentation-of chromosomal DNA, 1422Alpha subunit-of P. putida tryptophan synthetase, 248Amino acid- -naphthylamide

-hydrolysis by P. aeruginosa, 809Amino acids-aromatic, 386, 400-of N. rubra cell wall, 941Amino acid starvation-in E. coli, 263-polysome turnover during, 263Amino acid transport-in Valr mutants, 1034Aminoacyl-transfer RNA synthetases-regulation of synthesis, 254Ampicillin-resistant mutants-of E. colt, 1210Amylase activity-regulation of, 1205Anaerobic, dark growth-of R. rubrum, 1348Anaerobic dehydrogenase-genetic analysis, 1224-of E. coli, 1224Anaerobic growth-mutants obtained after, 1348Antibacterial nitroacridine-binding to nucleic acids, 1026-effect on bacterial growth, 320-effect on macromolecular biosynthesis, 320Antibiotic, 11.837 RP, 20Antibiotics-action on murein synthesis, 20-enzymatic inactivation of, 459Antigenic polysaccharide-from S. aureus mutant, 874Antigens-of 30S ribosomal proteins, 910Antimicrobial action-of hexachlorophene, 501D-Arabinose-enzymatic conversion of, 287, 293-growth on by A. aerogenes, 287- isomerization of, 293-metabolism by A. aerogenes, 293L-Arabinose operon-control of, 1-in E. coli, 1Arizona-nutritional requirements, 948Aromatic acid catabolism-enzymes of, 1257, 1270Aromatic amino acids-control by tyr gene, 386, 400-in E. coli, 386, 400-repression of biosynthesis, 386Arthrobacter-methylene hydroxylase activity, 1141Arylamidase-from P. aeruginosa, 809Aspartokinases

iv

SUBJECT INDEX

-in B. subtilis, 1129Aspergillus nidulans-energy substrate utilization, 309-glucosamine-requiring mutant of, 184-hyphal wall synthesis in, 184-maintenance requirement, 309-protein synthesis inhibition in 184Autolysins-killing of bacteria, 1235Autoradiography-studies of cell division, 3754-Azaleucine-inhibition of S. typhimurium growth, 95-mode of action of, 95-reversal by leucine of effects, 95

Bacillus-binding of radioactive benzylpenicillin, 662-nicotinic acid oxidation, 751, 757Bacillus cereus-dormant spore, 868-microstructure, 515-spore coat layers, 571Bacillus cereus spore-macromolecular sieving by, 868Bacillus larvae-fastidiousness of, 828-glucose catabolism in, 828-oligosporogeny, 828Bacillus licheniformis-autolysin-deficient mutants, 1235Bacillus megaterium-action of hexachlorophene on, 482, 492-leakage after hexachlorophene treatment, 492-microstructure, 515-mutant spores of, 817-poly-p-hydroxybutyrate from, 782-spores, 111-synthesis of 8-CNA in, 132Bacillus mycoides-microstructure, 515Bacillus pumilis-PATE in, 166Bacillus stearothermophilus-reassociated ribosomes, 589-ribosomal subunits, 589Bacillus subtilis-acrylamide gel electrophoresis studies of, 720-action of hexachlorophene on, 501-alkaline phosphatase activity, 928-aspartokinases in, 1129-compartmentalization of DNA synthesis, 1250-competent state, 668-cytochrome system, 652-division mutants, 1366-DNA polymerase in, 364-effect of zorbamycin, 30-fiber-structured material, 579-flagellar hooks of, 282-freeze-treated, 1250-macromolecular synthesis, in, 668-methyl methanesulfonate-sensitive mutant of, 364-protein composition, 720-respiratory pathways, 652

-sporulation, 720-sporulation mutants, 241-transforming DNA in, 680-tyrosyl-tRNA synthetase, 586-uptake of transforming DNA by, 38-vegetative cells, 928Bacitracin-effect on hemolysis by S. zymogenes, 844Bacterial cell division-regulation, 1296Bacterial deoxyribonucleic acid-role in tumorigenesis, 973-transfer to plant tissue, 973Bacterial protoplasts-action of hexachlorophene on, 501Bacterial systems, fractionated, 1017Bactericidal antibiotics, 1235Bactericidal compound-methylglyoxal, 1338Bacteroides melaninogenicus-growth on succinate, 175-growth requirements, 175Bacteriophage P2-eduction mediated by, 695Bacteriophage receptors-O-acetyl groups as component, 584-of cell walls, 1402-on S. aureus cell walls, 584Bacteriostatic antibiotics, 1235Beneckea nigrapulchrituda-sp. n., 1380Benzoic acid-bacterial growth on, 89-conversion to catechol, 89-metabolism by bacteria, 89Benzpyrene-3,4-'H, 202Bifidobacterium strains-fatty acid composition, 861Binding sites on polymers-Nitroakridin 3582, 1026Biosynthesis of 7, 8-diaminopelargonic acid, 1135Biosynthetic rate-of vitamin B6, 1001Boticin S55-effect on C. botulinum, 526-effect on ultrastructure, 526Branched-chain amino acids-of E. coli, 254Brucella abortus-electron microscopy of, 535-infection of hamster-kidney cells, 535-L-forms of, 535

C,9 cyclopropane acid-in T. thioxidans, 992C-29 hydrocarbons-of S. lutea, 213Caffeic acid metabolism-in humans, 996Candida lipolytica-genetic recombination in, 609-hybrid strains of, 609Carbohydrate mutants-of E. coli, 690

VOL. 108, 1971 v

SUBJECT INDEX

Carboxylic acids-effect of lactate dehydrogenases, 1290Cardiolipin-from S. aureus, 219Cardiolipin metabolism-inhibition, 1065-in H. parainfluenzae, 1058, 1065Catechol-conversion of benzoic acid to, 89Cell cycle in yeast-new method to study, 1045Cell division, 74-autoradiographic studies of, 375-in E. coli, 375Cell envelope-fracture faces in, 474-of E. coli, 474Cell leakage-hexachlorophene-induced, 492Cell wall-fractionation of, 553-of E. coli, 553-of gram-negative bacteria, 1402-proteins of, 553-receptor sites for colicin in, 422-solubilization of, 553-structure of, 553chiD mutation-effect of molybdate, 854Chemolithotroph-T. ferrooxidans, 328, 334Chemotaxis-in P. aeruginosa, 596Chloramphenicol-effect on hemolysis by S. zymogenes, 844-effect on oxidative phosphorylation, 1017-stimulation of RNA synthesis by, 849Cholesteryl glucoside-biosynthesis by M. gallinarum, 986Chromatium strain D-nucleotide pools in, 954Chromatophores-isolation and fractionation of, 465-of R. spheroides, 465-proteins of, 465Chromosomal deoxyribonucleic acid-from E. coli, 1422Circular deoxyribonucleic acid-from S. typhosa hybrids, 1357Citrobacter-nutritional requirements, 948Cladosporium resinae-fatty acid composition of, 777-grown on glucose and hydrocarbons, 777Class II mutants-ampicillin-resistant, 1210-of E. coli, 1210Clindamycin-inactivation of, 459-phosphorylation by S. coelicolor Muller, 459Clostridium botulinun-activation of type B toxin, 1051-effect of boticin S51, 526-structural changes, 526

Clostridiwn sordelli-spore appendages, 1384-taxonomy, 1384Clostridiwn thermoaceticum-synthesis of acetate by, 770Cold osmotic shock-in S. cerevisiae, 451Colicin-localization of receptor sites for, 422-neutralization by E. coli, 422-receptor sites in E. coli, 422Colicin production-by colicinogenic bacteria, 10-effect of protein synthesis inhibition, 10-induction by high temperature, 10Competent state-development of, 668Corynebacterium hofmanni-microstructure, 515Crystal violet-effects on E. coli, 1296Cyanide-resistant respiration-in N. crassa, 1087,O-Cyanoalanine-formation of by O-acetylserine sulfhydrylase, 132-in B. megaterium, 1323, 5-Cyclohexadiene-1, 2-diol-1-carboxylic acid, in-

termediate in benzoic acid metabolism, 89D-CyclOserine-effect on hemolysis by S. zymogenes, 844-lysis of B. licheniformis, 1235Cysteine-role in jl-CNA synthesis, 132Cytochromes-in B. subtilis, 652Cytoplasmic membrane-alterations in, 564-of E. coli, 545-proteins of, 545, 564-solubilization by Triton X-100, 545

Dark growth-mutants obtained after, 1348Denitrification-by Hyphomicrobium, 733-with methanol, 733Deoxycholate-disrupted oxidases-M. lysodeikticus membranes, 9643-Deoxy-D-arabinoheptulosonic acid 7-phosphate

synthetase-in E. coli K-12 mutants, 400-synthesis of, 400Deoxyribonucleic acid-base pools, 74-calf thymus, 202-chromosomal, 1422-covalent linkage with polycyclic hydrocarbons, 202-degradation by zorbamycin, 30-denaturation of by hydrocarbons, 202-episomal, 300-from E. coli, 1422-inhibition of template activity, 202-interstrand cross-links in, 202-loss of transforming activity, 202

V1 J. BAmrRioL.

SUBJECT INDEX

-mutation of, 202-of E. coli R+ minicells, 300-replication, 74-resistance to deoxyribonuclease, 38-resistance to EDTA, 38-transforming, 38, 680-uptake by B. subtilis, 38-uptake inhibition by EDTA, 38Deoxyribonucleic acid polymerase-impaired activity, 364-in B. subtiuis mutant, 364Deoxyribonucleic acid synthesis-in B. subtilis, 1250Developmental regulation-of amylase activity, 1205Diaminopelargonic acid-biosynthesis of, 1135Dictyostelium discoideum-nucleotide metabolism in, 2691,2-Dihydro-1,2-dihydroxybenzoic acid, intermediate

in benzoic acid metabolism, 89Dimethyl-1 ,2-benzanthracine-9, 10-IH, 202Direct selection-for transduction, 1427Division mutants-of B. subtilis, 1366Division-specific markers-PBS-1 transduction of, 1366

Eduction, 695Electron microscopy-of a B. abortus infection, 535-of rod-shaped bacteria, 515-of Trichoderma colonies, 938Embden-Meyerhof-Pamas pathway-in B. larvae, 828Enduracidin, 20Energy charge-in E. coli, 1072Energy substrate utilization- in A. nidulans, 309Entner-Doudorroff pathway-in B. larvae, 828envA gene-in E. coli K-12, 45, 51-mediating decreased tolerance to antibacterial

agents, 51-mediating sensitivity to gentian violet, 45EnvA phenotype-in E. coli, 51-inhibition ofRNA synthesis in, 51-suppression of, 51-tolerance to antibacterial agents, 51Enzyme alterations-in phe auxotrophs, 1174-in S. typhimurium, 1174-in tyr auxotrophs, 1174Enzyme formation-constitutive, 1147-induced, 1147Enzyme synthesis-regulation in B. subtilis, 586Equilibrium constant-for pyruvate conversion, 1107

Ergosterol, 69Escherichia coli-adenylate energy charge, 1072-ampicillin-resistant mutants, 1210-a syndrome of, 1322-autoradiographic studies in, 375-branched chain amino acids, 254-cell division in, 375-cell wall of, 553-chain formation in, 375-chiD mutants of, 854-chlorate-resistant mutants of, 564-constitutive glp mutants of, 137-crosses with S. typhimuriwn, 1161-ctr mutant, 690-cytoplasmic membrane of, 545, 564-deamidation of nicotinamide, 191-deletions in eductants of, 695-DNA of, 300-effects of crystal violet on, 1296-fertility, 1161-freeze-fractured cell envelope of, 474-gal region in, 5-gamma-ray damage to, 944-genetic determinant, 1424-giant cells, 1390-glycerol metabolism in, 137-L-a-glycerophosphate dehydrogenase of, 1224-growth on short-chain fatty acids, 885, 893-in rat stomach, 191-lac region in, 5-leucyl-tRNA synthetase in, 742-lipopolysaccharide, 1412-macarbomycin sensitivity in, 946-metabolism of 4-N-hydroxy-cytidine, 145-methionineless death, 1168-methylglyoxal produced by, 137, 1338-microstructure, 515-minicells of, 300-mutant, 1338-mutant alanyl-tRNA synthetase, 1008-nitrate reductase activity, 854-phosphorolysis of FUdR in, 760-PolA+ and PolA- strains, 1422-rel+ tRNA synthetase mutant of, 849-R factors of, 300-ribonuclease II in, 601-ribosomal proteins, 910-seryl-tRNA synthetase in, 742-sex-pili formation, 946-temperature-sensitive mutants of, 742-thymine-binding protein of, 1419-thymine starvation, 1422-transfer of R factors in, 508-tryptophan enzymes, 1181-tryptophan operon of, 615-vitamin Be synthesis in, 1001Escherichia coli B-compared to E. coli K-12, 105-growth rates of, 105-ornithine transcarbamylase in, 645-pyridoxineless mutants of, 415-ribosomes in, 105-vitamin B6 synthesis in, 415

* a

VOL. 108, 1971

SUBJECT INDEX

Escherichia coli B/r-L-arabinose operon in, 1-DNA-RNA hybridization studies in, 1Escherichia coll B/r/1-nucleoside triphosphate pools in, 74-synchronous cultures, 74Escherichia coli Hfr donor-S. typhosa hybrids from, 1357Escherichia coli K-12-aromatic amino acid biosynthesis in, 386, 400-colicin production in, 10-colicin receptor sites in, 422-colicin sensitivity in, 422-colicin tolerance in, 422-compared to E. coli B, 105-DAHP synthetase in, 400-envA gene in, 45, 51-growth rates of, 105-inhibition of RNA synthesis in, 51-macromolecular inhibition in, 45-penetration barrier in, 45-phenylalanine biosynthesis in, 400-phosphoglucomutase mutants, 621-potassium transport loci, 639-ribosomes, 105-tyrosine biosynthesis in, 400-tyrR mutants of, 386, 400-uptake of gentian violet, 45Escherichia colt mutants-amino acid transport, 1034-growth characteristics, 885-KDGP, 1277-temperature-sensitive, 790-TS for RNA regulation, 627-unable to make protein at 42 C, 790Escherichia operator-recognition by Salmonella repressor, 1431Ethidium bromide-effect on hemolysis by S. zymogenes, 844-effects on growth, 1200Ethylenediaminetetraacetic acid-inhibition of DNA transformation, 38Exocellular acid phosphatase-in S. mellis, 1097

Fatty acid composition-of Bifidobacterium strains, 861-of C. resinae, 777-of Lactobacillus strains, 861Fatty acids-genetic studies with, 353-in Micrococcacae, 353-of T. thiooxidans, 992Fatty acids, short-chain-growth of E. coli on, 885, 893Ferrous-ferric ion-formation of mycobactin, 314Fertility of Salmonella typhimurium-in crosses with E. coli, 1161Fertility repressor-subunits, 582Fiber-structured material-in B. subtilis fraction, 579Flagellar hooks

-characterization of, 282-of B. subtilis, 282-purification of, 282Flavobacterium peregrinum-deamidation of nicotinamide, 191-in rat stomach, 1915-Fluoro-2'-deoxyuridine-inhibition by nucleosides, 760-phosphorolysis in E. coli, 760Formate-conversion of pyruvate to, 1107Fractionated bacterial systems-oxidative phosphorylation in, 1017Freeze fracturing-of E. coli, 474Freeze treatment-of B. subtilis, 1250Fruiting-of S. commune, 1205Fruiting body formation-enzyme activity during, 1205L-Fucose isomerase-mutant form of, 293

Galactose genes-in S. cerevisiae, 179-order of, 179Galactose locus-in E. coli, 5-transposition of lac operon to, SB-Galactosidase formation-constitutive, 1147-inducible, 1147Galacturonate-mutants unable to grow on, 1277Gamma-aminobutyric acid-required germinant, 817Gamma-ray damage-RecA+-dependent repair of, 944Gastrointestinal tract of man-caffeic acid metabolism in, 996Gas-vacuolated strains-of M. aquaticus, 236Genetic analysis-of an E. coli syndrome, 1322Genetic complementation-repressor-negative mutants, 582Genetic determinant-in E. coli, 1424Genetics of mandelate pathway-in P. aeruginosa, 1270Giant cells-of E. coli, 1390Gluconate negativity-mutation causing, 1277Glucose-growth of C. resinae on, 777Glucose catabolism-in B. larvae, 828-pathways of, 828Glucose metabolism-in T. ferrooxidans, 334Glucose-6-phosphate dehydrogenase-in T. ferrooxidans, 343

*iii J. BACIERIO.

SUBJECI INDEX

-regulation of, 343Glucose utilization-by T. ferrooxidans, 328Glucuronate-mutants unable to grow on, 1277Glycerol-conversion to dihydroxyacetone, 592-oxidation by A. suboxydans, 592Glycerol metabolism-in E. coli, 137-unregulated, 137Glycerol transport-binding protein for, 82-induced, 82-in P. aeruginosa, 82L-a-Glycerophosphate dehydrogenase-anaerobic, 1224Glycolipids-of M. gallinarum, 986Gram-negative bacteria-cell walls of, 1402-effect of polymyxin on cell walls, 1402

Hamster-kidney cell culture-electron microscopy of, 535-infected by B. abortus, 535Haemophilus parainfluenzae-cardiolipin metabolism, 1058, 1065-membrane cardiolipin, 1058Heat stabilities-of reassociated ribosomes, 589-of ribosomal subunits, 589Heme-role in growth of B. melaninogenicus, 175Hemolysis-by S. zymogenes, 844-effect of drugs on, 844Heterofermentative lactic acid bacteria, 1284Heterotrophic metabolism-of T. ferrooxidans, 334Heterotrophic vibrioid bacteria-containing gas vacuoles, 236Hexachlorophene-cell leakage caused by, 492-mode of antimicrobial action, 482, 492, 501-ultrastructural changes caused by, 482-uptake by B. megateriumn, 482Hfr donor-S. typhosa hybrids from, 1357hisT gene-in S. typhimurium, 410Histidine-regulation in S. typhimurium, 410Homofermentative lactic acid-bacteria, 1284Host deletions-in E. coli eductants, 695Hybridization studies-DNA-RNA, 1Hydrocarbons-growth of C. resinae on, 777-major C-29, 213-of S. lutea, 213Hydrostatic pressure-effect on amino acid uptake, 835

-effect on marine bacteria, 8354-N-Hydroxy-cytidine-metabolic pathways of, 145Hydroxykynureninase activity-in N. crassa, 902Hydroxylysine-effect on lysine synthesis, 715-inhibiting growth of Saccharomyces, 7156-Hydroxynicotinic acid hydroxylases-purification and properties, 751-source of oxygen atoms, 757Hyphae-chitin incorporation in, 184-of A. nidulans, 184-wall synthesis in, 184Hyphomicrobium-denitrifying strain, 733-growth on methanol, 733-isolation of strain, 733

Immunochemical studies-of R. spheroides chromatophores, 645Immunological studies-of L. casei and S. faecalis, 196-of malic enzymes, 196Inhibition-of cardiolipin-metabolism, 1065Inhibitors of proton-conduction, 1065Inorgamc pyrophosphatases- from photosynthetic bacteria, 1122Isocitrate dehydrogenase-bacterial, 824-NADP-specific, 824Isocitrate metabolism-electrophoretic characteristics, 824Isoleucine-requirement in S. typhimurium mutant, 359

7-Keto-8-aminopelargonic acid-7,8-diaminopelargonic acid from, 1135Klebsiella-polysaccharides of, 1304Kynureninase activity-in N. crassa, 902

,B-Lactamase-from S. typhimurium, 159-types of, 159Lactate dehydrogenases-NAD-dependent, 1284, 1290-NAD-independent, 1284, 1290Lactic acid bacteria-heterofermentative, 1284, 1290-homofermentative, 1284, 1290-lactate dehydrogenases, 1284, 1290Lactobacillus-extracellular nuclease in, 604-fatty acid composition, 861Lactobacillus acidophilus-deamidation of nicotinamide, 191-in rat stomach, 191-microstructure, 515Lactobacillus casei-immunological studies of, 196

iXVOL. 108, 1971

SUBJECT INDEX

-malic enzymes of, 196-relationship to S. faecalis, 196Lactose operon-in E. coli, 5-transposition to gal region, 5L component of lysin-in S. zymogenes, 804Leucine-synthesis by S. typhimurium, 95Leucine transfer ribonucleic acid-role in repression in S. typhimurium, 951Leuconostoc mesenteroides-lactate dehydrogenases, 1290L-forms-of B. abortus, 535Lipids-of T. aquaticus, 227-of T. thiooxidans, 612Lipids of Thermus aquaticus-effect of growth-temperature, 227Lipid synthesis-effect of growth-conditions, 1310

-effect of mutations, 1310-in N. crassa, 1310Lipopolysaccharide-of E. coli, 1412Lysin-L-component, 804-of S. zymogenes, 804Lysine-synthesis inhibited by hydroxylysine, 715-synthesis in Saccharomyces, 715Lysis-of a psychrophilic marine bacterium, 916Lysylphosphatidylglycerol-from S. aureus, 219

Macarbomycin sensitivity-in E. coli, 946-relation to sex-pili formation, 946Macroconidia-in M. gypseum, 276-regulation of germination, 276Macromolecular biosynthesis-effect of Nitroakridin 3582, 1026-in B. subtilis, 668-in intact streptococci, 980-in protoplasts, 980Macromolecular sieving-by dormant spore, 868-in B. cereus, 868Mandelate pathway-in P. aeruginosa, 1257, 1270Marine bacteria-amino acid uptake in, 835-lysis of, 916-pyschrophilic, 835, 916-structural changes, 916-taxonomy of, 1380-temperature and pressure studies of, 835Membrane cardiolipin-in H. parainfluenzae, 1058Membrane oxidases-deoxycholate-disrupted, 964

-of M. lysodeikticus, 964Membranous structure-lipopolysaccharide of, 1412-of E. coli, 1412Metabolism-of poly-jl-hydroxybutyrate, 782Metabolism of caffeic acid-in humans, 996Metabolism of cardiolipin-heterogeneity, 1058-in H. parainfluenzae, 1058, 1065Metal ions-formation of mycobactin, 314Methanol-growth on by Hyphomicrobium, 733-in denitrification, 733Methionineless death-in E. coli, 1168Methylene hydroxylase activity-of Arthrobacter, 1141-structural effects, 1141Methylglyoxal-lethal synthesis by E. coli, 137-mode of action, 1338-produced by E. coil mutant, 1338-properties, 1138Methyl methanesulfonate-sensitive B. subtilis mutant, 364Methyltetrahydrofolate-in acetate synthesis, 770Micrococcaceae-neutral lipids of, 353-relationships among family members, 353Micrococcus-relationship to Staphylococcus, 353Micrococcus luteus-relationship to other Micrococcacae, 353Micrococcus lysodeikticus-deoxycholate-disrupted membrane oxidases, 964-restoration of oxidases, 964Microcyclus aquaticus-gas-vacuolated strains, 236Microsporum gypseum-macroconidia germination, 276Microstructure-rod-shaped bacteria, 515Minicells-DNA in, 300-of E. coli, 300Moenomycin, 20Molecular seive chromatography-for isolation of chromatophores, 465Molybdate-effect on chiD mutation, 854Multiseptation-mathematically derived model for, 607-Paulton model for, 607Murein synthesis-inhibition by antibiotics, 20Mutant-of R. rubrum, 1348Mutant alanyl-tRNA synthetase-in E. coli, 1008Mutant of Escherichia coli

x J. BACrERIOL.

SUBJECT INDEX

-amino acid transport, 1034-ctr, 690-KDGP, 1277-unable to make protein at 42 C, 790Mutant of Staphylococcus aureus-surface antigenic polysaccharide from, 874Mutant spores-germination of, 817-of B. megaterium, 817Mycobacterium phlei-site of chloramphenicol action, 1017Mycobacterium smegmatis-action of rifampin on, 737-formation of mycobactin in, 314-formation of salicylic acid in, 314-RNA polymerase inhibition in, 737Mycobactin formation-influence of metal ions on, 314-in M. smegmatis, 314Mycoplasma gallinarum-biosynthesis of cholesteryl glucoside by, 986-glycolipids of, 986Mycoplasma primatum sp. n., 439Mycoplasmas-simian, 439Mycoplasma strain Navel-characteristics of, 439-new classification for, 439-relationship to other mycoplasmas, 439

Neurospora crassa-cyanide-resistant respiration, 1087-fatty acids, 1310-germination of, 446-hydroxykynureninase activity in, 902-kynureninase activity in, 902-lipid synthesis in, 1310-tRNA methylases in, 446-trehelaseless mutants of, 59Nicotinamide-deamidation by microorganisms, 191Nicotinic acid-oxidation by a Bacillus sp., 751, 757-purification and properties, 751Nitrate medium, semisolid, 596Nitroacridine, antibacterial, 320Nitroakridin 3582-binding to nucleic acids, 1026-effect on bacterial growth, 320-effect on macromolecular synthesis, 320, 1026Nitrobacter agilis-distinction with N. winogradskyi, 1416Nitrobacter winogradskyi-distinction with N. agilis, 1416Nocardia graminis-microstructure, 515Nocardia rubra-amino acids of cell wall, 941Nonsulfur purple bacteria-pyrophosphatases from, 1122Nuclear protein-identified as actin, 705-in P. polycephalwn, 705Nuclease

-extracellular, 604-of Lactobacillus, 604Nucleoside triphosphates-in E. coli B/r/l, 74-pools of, 74-role in cell division, 74Nucleotide metabolism-during differentiation, 269-in D. discoideum, 269Nucleotide pools-in growing Chromatium strain D, 954Nystatin-binding of by sterol, 69-resistance to in yeast, 69

Operator-Escherichia, 1431Ornithine transcarbamylase-genetic control in E. coli B, 645Oxidation of nicotinic acid-by a Bacillus sp., 751, 757Oxidative phosphorylation-in fractionated bacterial systems, 1017

Paracrystal fractions-of Bacillus sp., 579Partial revertants,-of B. subtilis, 241Paulton multiseptation model-mathematical analysis of, 607PBS-1 transduction-of division-specific markers, 1366Pectic acid trans-eliminase-in B. pwnilis, 166Penetration barrier-in E. coli K-12, 45-permeability to gentian violet, 45Penicillin-resistance to, 1154Penicillinase-negative organism-S. aureus, 1154Penicillinase plasmid-curing by ethidium bromide, 1192, 1200-effects of recipient strain, 1192-of S. aureus, 1192, 1200Pentose phosphate pathway-in B. larvae, 828Peptidoglycan synthesis-inhibition by antibiotics, 20Phenethyl alcohol-effect on cell penetration barrier, 51-increasing tolerance to antimicrobial agents, 51-inhibition of RNA synthesis by, 51Phenotypic restoration-by molybdate, 854-of nitrate reductase activity, 854Phenotypic reversion-in sporulation mutants, 241Phenylalanine auxotrophs-of S. typhimurium, 1174Phenylalanine biosynthesis-in E. coli K-12, 400Phosphatidylglycerol-from S. aureus, 219

XiVOL. 108, 1971

SUBJECT INDEX

Phosphoglucomutase-in E. coli K-12 mutants, 621Phosphonomycin-effect on hemolysis by S. zymogenes, 844Phosphoribosylpyrophosphate synthetase-control mechanism of, 122-in S. typhimurium, 122-metabolites derived from, 122Phosphorolysis-of FUdR, 760Phosphorylation, oxidative, 1017Photosynthetic bacteria-inorganic pyrophosphatases, 1122Phototrophic sulfur bacterium-adenylyl sulfate reductase from, 1112Physarum polycephalum-actin in, 705-nuclear protein of, 705Pigmentation-in S. marcescens, 765Polycyclic hydrocarbons-carcinogenic, 202-effect on DNA activities, 202Polygalacturonic acid-degradation by PATE, 166-inducing PATE, 166Poly-,B-hydroxybutyrate-metabolism of, 782Poly-,3-hydroxybutyrate granules-mild alkaline extraction, 782Polymyxin-effect on phage receptors, 1402Polysaccharides-of type 6 Klebsiella, 1304Polysomes-in E. coli, 105, 263-turnover of, 263Potassium transport-in E. coli K-12, 639Prasinomycin, 20Progenitor toxin-activation by a purified enzyme, 1051Protease-alkaline, 276-regulation of macroconidia germination by, 276Protein-of E. coli, 790, 1419-thymine-binding, 1419Protein synthesis-after various treatments, 1147-inhibition inducing colicin production, 10Protoplasts-of S. faecalis, 980Providencia-nutritional requirements, 948Pseudomonad-growth characteristics, 1318Pseudomonas aeruginosa-amino acid-,ONA metabolism in, 809-chemotactic response to amino acids, 596-glycerol transport in, 82-grown in semisolid nitrate medium, 596-mandelate pathway, 1257, 1270-R factor from, 1244

Pseudomonas cepacia-enzymes of, 799-zone electrophoresis of enzymes, 799Pseudomonas putida-compared to enteric bacteria, 431-tryptophan synthetase, 248, 431Pseudomonas solanaceareum-enzymes of, 799-zone electrophoresis of enzymes, 799Pseudomonas viscosa-microstructure, 515Pseudoplasmodium stage-nucleotide metabolism during, 269Psychrophilic marine bacterium- lysis, 916Pyridoxamine-as carbon source, 1318-utilization by a pseudomonad, 1318Pyridoxine-as carbon source, 1318-utilization by a pseudomonad, 1318Pyrimidine nucleotides-repression of PRPP synthetase by, 122Pyrophosphatases-catalytic properties of, 1122-from photosynthetic bacteria, 1122-regulatory diversity, 1122-R. capsulata, 1122-R. rubrum, 1122Pyruvate conversion-equilibrium constant for, 1107

R factor-class P, 1244-F-like, 582-8-lactamases of, 159-mechanism of transfer, 508-of E. coli, 508-of E. coli minicells, 300-of P. aeruginosa, 1244-of Shigella, 159-of S. typhimurium, 159-physical properties, 508Radioactive benzylpenicillin-binding to Bacillus, 662Radioactive precursors-incorporation by protoplasts, 980Rate of vitamin Be biosynthesis-role in vitamin Be synthesis, 1001Reassociated ribosomes-heat stabilities, 589RecA+-dependent repair-of gamma-ray damage, 944Recombination-between E. coli chromosomes, 944Reconstitution in vitro-of spore coat layers, 571Regulation-of bacterial cell division, 1296Regulation of aspartokinase-in B. subtilis, 1129Regulation of enzyme synthesis-in B. subtilis, 586Regulation of mandelate pathway

xi J. BACnuuoL.

SUBJECT INDEX

-in P. aeruginosa, 1257Regulation of synthesis-aminoacyl-tRNA synthetases, 254Repression-in S. typhimuriwn, 951-role of leucine tRNA, 951Repressor-Salmonella, 1431Repressor-negative mutants-genetic complementation, 582Respiration-cyanide-resistant, 1087-in N. crassa, 1087Respiratory chains-M. phlei, 1017Restoration of disrupted oxidases-in M. Iysodeikticus membranes, 964Rhodopseudomonas species-pyrophosphatases from, 1122Rhodopseudomonas spheroides-chromatophores of, 465Rhodospirillum rubrum-mutants, 1348-pyrophosphatases from, 1122Ribonuclease II-in E. coli, 601-inhibition of, 601Ribonucleic acid-degradation by zorbamycin, 30-synthesis in E. coli ts mutants, 627Ribonucleic acid polymerase-inhibition by rifampin, 737-in M. smegmatis, 737Ribosomal proteins-antigenic similarities, 910-of E. coli, 910Ribosomal subunits-heat stabilities of, 589Ribosomes-in E. coti, 105-production of, 105-subunit pools, 105Rifampin-action on M. smegmatis, 737-inhibition of RNA polymerase by, 737Rod-shaped bacteria-microstructure, 515

Saccharomyces-effect of hydroxylysine on growth of, 715-lysine synthesis in, 715Saccharomyces cerevisiae-cell cycle, 1045-cold osmotic shock in, 451-compared to N. crassa, 451-gal genes in, 179Saccharomyces mellis-acid phosphatase of, 1097Salicylic acid formation-influence of metal ions on, 314-in M. smegmatis, 314Salmonella-linkage of supD tofla genes, 1427-suppressor mutations, 1427

Salmonella pullorum-sulfate-using revertants of, 154-sulfite reductase in, 154-temperature-sensitive defect in, 154Salmonella repressor-recognition of Escherichia operator, 1431Salmonella typhimurium-crosses with E. coli, 1161-enzyme activities in, 95-enzyme alterations, 1174-fertility, 1161-histidine regulation in, 410-hisT mutants of, 410-inhibition of growth by 4-azaleucine, 95-isoleucine requirement in, 359-leucine metabolism in, 95-leucine tRNA in repression, 951-LT2, 159-nutritional mutants of, 359-PRPP synthetase in, 122-R factors of, 159-tyr and phe auxotrophs, 1174-vitamin B6 requirement in, 359Salmonella typhosa hybrids-circular DNA from, 1357Sarcina lutea-hydrocarbons of, 213Schizophyllum commune-fruiting of, 1205-regulation of amylase activity, 1205Schizosaccharomyces pombe-adenine aminohydrolase in, 959Serine-role in ,B-CNA synthesis, 132Serratia marcescens-acriflavine resistance in, 765-microstructure, 515-pigmentation in, 765Sex pili-in E. coli, 946-relationship to macarbomycin sensitivity, 946Shigella-R factors of, 159Short-chain fatty acids-growth of E. coli on, 885, 893Size separation of yeast, 1045Slime mold-nuclear protein of, 705Sodium ions-inhibition of ribonuclease II, 601Sorocarp stage-nucleotide metabolism during, 269Spore appendages-of C. sordellii, 1384Spore coat layers-B. subtilis, 571-reconstitution in vitro, 571Spore germination-y-aminobutyric acid requirement, 817-in B. megateriwn, 111, 817Spores-heat resistance of, 1 11-macromolecular sieving by, 868Spores

xiiiVOL. 108, 1971

SUBJECT INDEX

-of B. megateriwn, 111-of B. cereus, 868Sporulation-changes in protein composition during, 720-in B. subtilis, 241, 720-mutant, 241-studied by acrylamide gel electrophoresis, 720Staphylococcus-relationship to Micrococcus, 353Staphylococcus aureus-cardiolipin from, 219-cell wall phage receptor, 584-lysylphosphatidylglycerol from, 219-mutants, 874, 1154-penicillinase plasmids, 1192, 1200-phosphatidylglycerol from, 219-resistance to penicillin, 1154-surface polymer from, 874Sterols-binding of nystatin, 69-inhibition of fungicidal effects by, 69-in yeast, 69Streptococcus faecalis-deamidation of nicotinamide, 191-growing protoplasts of, 980-immunological studies of, 196-incorporation of radioactive precursors, 980-in rat stomach, 191-malic enzymes of, 196-relationship to L. casei, 196-synthesis of radiolabeled macromolecules, 980Streptococcus zymogenes-effect of drugs on, 844-hemolytic activity of, 844-L component of lysin, 804-X14 mutant, 804Streptomyces coelicolor Muller-phosphorylation of clindamycin by, 459Streptomycin-effect on hemolysis by S. zymogenes, 844Structural changes-caused by boticin S51, 526Substrate specificity-of mutant alanyl-tRNA synthetase, 1008Subunits-in fertility repressor, 582Succinate-role in growth of B. melaninogenicus, 175Sulfate-reducing bacteria-adenylyl sulfate reductases from, 1112Sulfite reductase-in S. pullorum, 154-thermolability of, 154Sulfur-"wetting agent" for, 612Suppressor mutations-in Salmonella, 1427Synthesis, macromolecular-in B. subtilis, 668Synthesis of acetate-from C02, 770Synthesis of amino acids-regulation of, 254

Taxonomy-of Clostridium sordelli, 1384Temperature of growth-effect on lipids of T. aquaticus, 227Temperature sensitivity-of tRNA synthetases, 742, 849-in E. coli, 627, 742, 849Thermophile ribosomes-heat stability of, 589Thermus aquaticus- lipids of, 227Thiamine-binding protein-of E. coli, 1419Thiobacilli-adenylyl sulfate reductase from, 1112Thiobacillus ferrooxidans-glucose utilization by, 328-G-6-P dehydrogenase in, 343-heterotrophic metabolism of, 334Thiobacillus thiooxidans-extracellular lipid of, 612-fatty acids of, 992-growth on sulfur, 612Thiocapsa roseopersicina-adenylyl sulfate reductase from, 1112Thymineless death-genetic-locus affecting, 1424-in E. coli, 1424Thymine starvation-of E. coli, 1422Toxin activation-botulinal, 1051Transducing bacteriophages-Xlac, 5Transduction-direct selection for, 1427-of suppressor mutations and linkage, 1427Transduction kinetics-of penicillinase plasmid, 1192Transfer ribonucleic acid-inhibition of ribonuclease II, 601Transfer ribonucleic acid methylases-in N. crassa, 446-role in germination, 446Transfer ribonucleic acid synthetase-in E. coli, 742, 849-leucyl, 742-seryl, 742-stimulated by chloramphenicol, 844-temperature-sensitive, 742, 849-tyrosyl, 586-valyl, 849Transforming deoxyribonucleic acid-in B. subtilis, 680Transposition-lac genes to gal region, 5Trehalase-control of, 59-lack in N. crassa, 59Trehalose-utilization by N. crassa, 59Trichoderma colonies-scanning electron microscopy, 938

XiV J. BAcTERioL.

SUBJECT INDEX

Triton X-100-solubilization of cell wall with, 553-solubilization of cytoplasmic membrane with, 545trpA gene-transcription of, 615trpE gene-transcription of, 615Tryptophan enzymes-control of, 1181-of E. coli, 1181Tryptophan operon-genes of, 615-of E. coli, 615Tryptophan synthetase-components of, 248, 431-of P. putida, 248, 431-purification of, 431Tumor induction-by A. tumefaciens, 973tyr gene-control of aromatic amino acid synthesis, 386-in E. coli K-1, 386-isolation of, 386Tyrosine auxotrophs-of S. typhimurium, 1174Tyrosine biosynthesis-control of pathway, 400-inE. coli K-12, 400Tyrosyl-transfer ribonucleic acid synthetase-regulation of, 586

Ultraviolet irradiation-effect on transduction kinetics, 1192Ultraviolet sensitivity

-in E. coli, 1424-locus affecting, 1424Uptake of butyrate-by E. coli K-12 mutants, 893Uptake of valerate-by E. coli K-12 mutants, 893

Valine inhibition-mutants resistant to, 1034Vancomycin-effect on hemolysis by S. zymogenes, 844-lysis of B. licheniformis, 1235Vitamin B6-control of synthesis of, 415-in E. coli B, 415-requirement in S. typhimurium mutant, 359-role of rate, 1001Vitamin K-role in growth of B. melaninogenicus, 175

Yeast-adenine aminohydrolase in, 959-cell cycle, 1045-cold osmotic shock in, 451-nystatin-resistant mutants of, 69-S. pombe, 959-sterol content of, 69

Zorbamycin-degrading DNA and RNA, 30Zonal centrifugation-to study cell cycle in yeast, 1045Zone electrophoresis of enzymes-comparative, 799

VOL. 108, 1971 xv

JOURNAL

OF

BACTERIOLOGY

VOLUME 108

BALTIMORE, MD. 21202

1971

JOURNAL OF BACTERIOLOGYVOLUME 108

EDITORIAL BOARD

L. LEON CAMPBELL, Editor-in-Chief (1975)University ofIllinois, Urbana

R. L. ANDERSON, Editor (1975)Michigan State University, East Lansing

S. G. BRADLEY, Editor (1974)Virginia Commonwealth University, Richmond

ROY CURTISS III, Editor (1974)The University of Tennessee, Oak Ridge

ALLEN G. MARR, Editor (1973)University ofCalifornia, Davis

H. E. UMBARGER, Editor (1976)Purdue University, Lafayette, Ind.

Martin Alexander (1972)W. Lane Barksdale (1972)M. E. Bayer (1972)Claire M. Berg (1973)Robert W. Bernlohr (1973)Allan Campbell (1972)A. J. Clark (1972)Royston C. Clowes (1972)V. W. Cochrane (1972)Stanley N. Cohen (1973)S. F. Conti (1972)A. L. Demain (1972)John A. DeMoss (1972)Walter J. Dobrogosz (1973)Martin Dworkin (1973)Alan D. Elbein (1973)Herman Friedman (1972)A. T. Ganesan (1972)Howard Goldfine (1972)Joseph S. Gots (1972)Harlyn 0. Halvorson (1972)F. M. Harold (1973)

Leonard Hayflick (1972)George Hegeman (1971)Charles E. Helmstetter (1972)Joseph T. Holden (1972)John G. Holt (1972)Stanley C. Holt (1971)John Ingraham (1973)John H. Law (1971)Hillel S. Levinson (1972)W. R. Lockhart (1972)Donald G. Lundgren (1971)Manley Mandel (1972)Paul Margolin (1972)Alvin Markovitz (1973)Julius Marmur (1971)Robert G. Martin (1971)M. L. Morse (1972)Richard P. Novick (1973)Leo Parks (1973)H. J. Phaff (1972)Allen T. Phillips (1973)Jack Preiss (1971)Charles C. Remsen III (1973)

William S. Reznikoff (1973)H. V. Rickenberg (1973)Antonio H. Romano (1973)John Roth (1973)M. R. J. Salton (1972)Moselio Schaechter (1973)David Schlessinger (1971)Simon Silver (1973)W. R. Sistrom (1972)Lucile Smith (1972)Bernard S. Strauss (1972)Noboru Sueoka (1972)Paul S. Sypherd (1971)A. L. Taylor (1971)H. E. Umbarger (1973)Arthur Weissbach (1971)N. E. Welker (1973)R. W. Wheat (1972)Helen R. Whiteley (1972)Meyer J. Wolin (1973)Barbara E. Wright (1971)Frank E. Young (1973)Stanley A. Zahler (1971)

ROBERT A. DAY, Managing Editor, 1913 I St., N. W., Washington, D.C. 20006

EX OFFICIOMORRIS F. SHAFFER, President (197 1-1972)

DONALD E. SHAY, Secretary

The Journal of Bacteriology, a publication of the American Society forMicrobiology, 1913 1 St., N.W., Washington, D.C. 20006, is devoted tothe advancement and dissemination of fundamental knowledge concerningbacteria and other microorganisms. The Journal is published monthly, andthe twelve numbers are divided into four volumes per year. The subscrip-tion price is $52 (Postal Union Countries, $54; other foreign, $55) peryear. Single copies are $5.00 (foreign, 5.25). Members of the AmericanSociety for Microbiology may receive the Journal as part of their dues.Correspondence relating to reprints, defective copies, availability of backissues, lost or late proofs, disposition of submitted manuscripts, andgeneral editorial matters should be directed to the ASM PublicationsOffice, 1913 I St., N.W., Washington, D.C. 20006. Correspondence from

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changes of address, incorrect journals, etc., should be directed to theExecutive Secretary, American Society for Microbiology, 1913 1 St., N.W.,Washington, D.C. 20006. Nonmembers of the Society should write to TheWilliams & Wilkins Co., 428 E. Preston St., Baltimore, Md. 21202, regard-ing institution of nonmember subscriptions and nonreceipt ofjournals. Pub-lished monthly by the ASM at 428 E. Preston St., Baltimore, Md. 21202.

Second class postage paid at Baltimore, Md. 21202, and at additionalmailing offices.Made in the United States of America.Copyright ) 1971, American Society for MicrobiologyAll Rights Reserved.

Volume 108 Contents for October

Taxonomy and Ecology

Detection of Relationships Between Streptococcus faecalis and Lactobacillus casei byImmunological Studies with Two Forms of Malic Enzyme. JACK LONDON,ELEANOR Y. MEYER, AND SANDRA R. KULCZYK.......................... 196

Gas-Vacuolated Strains of Microcyclus aquaticans. M. VAN ERT AND J. T. STALEY. 236

Neutral Lipids in the Study of Relationships of Members of the Family Micrococcaceae.SUSAN J. MORRISON, T. G. TORNABENE, AND WESLEY E. KLooS............. 353

Proposal for Classifying Human Strain Navel and Related Simian Mycoplasmas asMycoplasma primatum sp. n. RICHARD A. DELGIUDICE, THEODORE R. CARSKI,MICHAEL F. BARILE, RUTH M. LEMCKE, AND JOSEPH G. TULLY................ 439

Morphology and UltrastructureHyphal Wall Synthesis in Aspergillusnidulans: Effect of Protein Synthesis Inhibition and

Osmotic Shock on Chitin Insertion and Morphogenesis. DVORAH KATZ ANDR. F. ROSENBERGER ..................................................... 184

Purification and Partial Characterization of Bacillus subtilis Flagellar Hooks. K.DIMMITT AND M. SIMON................................................ 282

Fracture Faces in the Cell Envelope of Escherichia coli. A. P. VAN GOOL AND N. NAN-...................................................................474

Microstructure of Colonies of Rod-Shaped Bacteria. D. B. DRUCKER AND D. K.WHITTAKER ............................................................ 515

Structural Changes in Clostridium botulinum Type E After Treatment with Boticin S51 .

JANE S. ELLISON, CARL F. T. MATTERN, AND WENDELL A. DANIEL.............. 526

Electron Microscopy of Tissue Culture Cells Infected with Brucella abortus. BETTYA. HATTEN, SHYI Yi HUANG, M. L. SCHULZE, AND S. EDWARD SULKIN......... 535

Solubilization of the Cytoplasmic Membrane of Escherichia coli by Triton X-100.CARL A. SCHNAITMAN................................................... 545

Effect of Ethylenediaminetetraacetic Acid, Triton X-100, and Lysozyme on theMorphology and Chemical Composition of Isolated Cell Walls of Escherichiacoli. CARL A. SCHNAITMAN............................................. 553

Alterations in the Cytoplasmic Membrane Proteins of Various Chlorate-ResistantMutants of Escherichia coli. C. H. MACGREGOR AND C. A. SCHNAITMAN....... 564

Reconstitution of Bacterial Spore Coat Layers In Vitro. A. I. ARONSON AND P. C.FITZ-JAMES ...................................5.71.. i4 ...... .. 571

Fiber-Structured Material in a Fraction of Bacillus subtilis. YUZURU HIRAGI ANDHAJIMEKADOTA. 579

Genetics and Molecular BiologyDeoxyribonucleic Acid-Ribonucleic Acid Hybridization Studies on the L-Arabinose

Operon of Escherichia coli B/r. GARY WILCOX, JUDITH SINGER, AND LAURELHEFFERNAN.1

Transposition of the lac Region to the gal Region of the Escherichia coli Chromosome:Isolation of Xlac Transducing Bacteriophages. K. IPPEN, J. A. SHAPIRO, ANDJ. R BECKWITH ........................................................ 5

Induction of Colicin Production by High Temperature or Inhibition of Protein Syn-thesis. C. K. KENNEDY ............ . .. . . . . . ................................ 10

In Vivo and In Vitro Action of New Antibiotics Interfering with the Utilization ofN-Acetyl-Glucosamine-N-Acetyl-Muramyl-Pentapeptide. E. J. J. LUGTENBERG,ARNA VAN SCHIJNDEL-VAN DAM, AND T. H. M. VAN BELLEGEM................. 20

Mode of Action of Zorbamycin. FRITZ REUSSER............................... 30

Early Intermediate State of Transforming Deoxyribonucleic Acid During Uptake byBacillus subtilis. D. A. MORRISON........................................ 38

Nature of the Penetration Barrier in Escherichia coli K-12: Effect of MacromolecularInhibition on Penetrability in Strains Containing the envA Gene. STAFFANNORMARK AND BRITTA WESTLING.......................................... 45

xix

Number 1

Phenethyl Alcohol as a Suppressor of the EnvA Phenotype Associated with the envAGene in Escherichia coli K-12. STAFFAN NORMARK......................... 51

Isolation, Mapping, and Characterization of Trehalaseless Mutants of Neurosporacrassa. ALFRED S. SUSSMAN, M. KERRY GARRETT, MALCOLM SARGENT, ANDSHIH-AN YU .....................59

Nystatin-Resistant Mutants of Yeast: Alterations in Sterol Content. ROBIN A.WOODS.................................................... ........... 69

Nucleoside Triphosphate Pools in Synchronous Cultures of Escherichia coli. LYDIAHUZYK AND D. JOSEPH CLARK............................................ 74

Transport of Glycerol by Pseudomonas aeruginosa. SAN-SAN TSAY, KAREN K.BROWN, AND ELIZABETH T. GAUDY........................................ 82

Genetic Order of the Galactose Structural Genes in Saccharomyces cerevisiae. JOHNBASSEL AND ROBERT MORTIMER................................. ......... 179

Mutagenic Action, Loss of Transforming Activity, and Inhibition of DeoxyribonucleicAcid Template Activity In Vitro Caused by Chemical Linkage of CarcinogenicPolycyclic Hydrocarbons to Deoxyribonucleic Acid. VERONICA M. MAHER,STEPHEN A. LESKO, JR., PATRICIA A. STRAAT, AND PAUL 0. P. Ts'o .......... . 202

Phenotypic Reversion in Some Early Blocked Sporulation Mutants of Bacillus subtilis:Isolation and Phenotype Identification of Partial Revertants. J. F. GUESPIN-MICHEL ............... .............. .... ............................. 241

Regulation of Synthesis of the Aminoacyl-Transfer Ribonucleic Acid Synthetases forthe Branched-Chain Amino Acids of Escherichia coli. E. MCGINNIS AND L. S.WILLIAMS............................................................... 254

Polysome Turnover During Amino Acid Starvation in Escherichia coli. ELIORAZ. RON............................................................... 263

Physical and Functional Characteristics of R-Factor Deoxyribonucleic Acid Segre-gated into Escherichia coli Minicells. STUART B. LEVY....................... 300

Salmonella typhimurium Mutants with Alternate Requirements for Vitamin B6 orIsoleucine. BEVERLY M. GUIRARD, BRUCE N. AMES, AND ESMOND E. SNELL ... . 359

Altered Deoxyribonucleic Acid Polymerase Activity in a Methyl Methansulfonate-Sensitive Mutant of Bacillus subtilis. KENNETH B. GASS, THOMAS C. HILL,MEHRAN GOULIAN, BERNARD S. STRAUSS, AND NICHOLAS R. COZZARELLI....... 364

On the Process of Cellular Division in Escherichia coli. VI. Use of a Methocel-Auto-radiographic Method for the Study of Cellular Division in Escherichia coli. E. C.C. LIN, Y. HIROTA, AND F. JACOB......................................... 375

Repression of Aromatic Amino Acid Biosynthesis in Escherichia coli K-12. K. D.BROWN AND R. L. SOMERVILLE............................................ 386

Phenylalanine and Tyrosine Biosynthesis in Escherichia coli K- 12: Mutants Derepressedfor 3-Deoxy-D-Arabinoheptulosonic Acid 7-Phosphate Synthetase (phe), 3-Deoxy-D-Arabinoheptulosonic Acid 7-Phosphate Synthetase (tyr), ChorismateMutase T-Prephenate Dehydrogenase, and Transaminase A. S. W. K. IM, H.DAVIDSON, AND J. PITTARD............................................... 400

Histidine Regulation in Salmonella typhimurium. VIII. Mutations of the hisT Gene.GEORGE W. CHANG, JOHN R. ROTH, AND BRUCE N. AMES..................... 410

Control of Vitamin B6 Biosynthesis in Escherichia coli. WALTER B. DEMPSEY....... 415Localization and Solubilization of Colicin Receptors. SOHAIR FABRID SABET AND

CARL A. SCHNAITMAN.................................................. 422Physical Properties and Mechanism of Transfer of R Factors in Escherichia coli.

DANIEL VAPNEK, MURIEL B. LIPMAN, AND W. DEAN RUPP .................... 508Genetic Evidence for Subunits in the Fertility Repressor Produced by F-Like R

Factors. DAVID I. HOAR............................................... 582O-Acetyl Groups as a Component of the Bacteriophage Receptor on Staphylococcus

aureus Cell Walls. D. R. D. SHAW AND ANADI N. CHATTERJEE............... 584Regulation of Tyrosyl-Transfer Ribonucleic Acid Synthetase in Bacillus subtilis.

BEVERLY A. DALE AND EUGENE W. NESTER................................. 586Heat Stabilities of Ribosomal Subunits and Reassociated Ribosomes from Bacillus

stearothermophilus. S. MARVIN FRIEDMAN................................. 589

xx CONTENTS

Complementation and Genetic Recombination in Candida lipolytica. JOHN BASSEL,JEAN WARFEL, AND ROBERT MORTIMER..................................... 609

Transcription of the Operator Proximal and Distal Ends of the Tryptophan Operon:Evidence that trpE and trpA Are the Delimiting Structural Genes. JOHN K.ROSE AND CHARLES YANOFSKY............................................ 615

Physiology and Metabolism

Metabolism of Benzoic Acid by Bacteria: 3,5-Cyclohexadiene-1 , 2- Diol-1- CarboxylicAcid Is an Intermediate in the Formation of Catechol. ALBEY M. REINER...... 89

Effect of 4-Azaleucine upon Leucine Metabolism in Salmonella typhimurium. B.STIEGLITZ AND J. M. CALVO .. 95

Ribosome Patterns in Escherichia coli Growing at Various Rates. FREDERICK VAR-RICCHIO AND ROGER MONIER............... .105

Distribution and Correlation of Events During Thermal Inactivation of Bacillusmegaterium Spores. HILLEL S. LEVINSON AND MILDRED T. HYATT ... ......... 1II

Regulation and Mechanism of Phosphoribosylpyrophosphate Synthetase: Repressionby End Products. MARY N. WHITE, JOYCE OLSZOWY, AND R. L. SWITZER. 122

Formation of 13-Cyanoalanine by O-Acetylserine Sulfhydrylase. PETER A. CASTRICAND ERICE. CONN .............................. 132

Lethal Synthesis of Methylglyoxal by Escherichia coli During Unregulated GlycerolMetabolism. W. B. FREEDBERG, W. S. KISTLER, AND E. C. C. LIN............. 137

Metabolism of 4-N-Hydroxy Cytidine in Escherichia coli. R. B. TRIMBLE AND FRANKMALEY............................. 145

Succinate as a Growth Factor for Bacteroides melaninogenicus. M. LEV, K. C.KEUDELL, AND A. F. MILFORD.......................175

Nicotinamide Deamidation by Microorganisms in Rat Stomach. MAKOTO SHIMO-YAMA, YOSHINORI TANIGAWA, TOICHI ITO, RYuzO MURASHIMA, IWAO UEDA, ANDTSUNESUKE TOMODA 191

Confirmation of the Identification of the Major C-29 Hydrocarbons of Sarcina lutea.PHILLIP W. ALBRO 213

Metabolism of Phosphatidylglycerol, Lysylphosphatidylglycerol, and Cardiolipin ofStaphylococcus aureus. STEVEN A. SHORT AND DAVID C. WHITE.............. 219

Effect of Growth Temperature on the Lipid Composition of Thermus aquaticus. PAULH. RAY, DAVID C. WHITE, AND THOMAS D. BROCK........................... 227

Nucleotide Metabolism During Differentiation in Dictyostelium discoideum. C. L.RUTHERFORD AND B. E. WRIGHT ........................ 269

Regulation and Self-Inhibition of Microsporum gypseum Macroconidia Germination.W. J. PAGE AND J. J. STOCK .......................... ................... 276

Relationship Between Energy Substrate Utilization and Specific Growth Rate inAspergillus nidulans. B. L. A. CARTER, A. T. BULL, S. J. PIRT, AND B. I. ROWLEY. 309

Influence of Metal Ions on the Formation of Mycobactin and Salicylic Acid in Myco-bacterium smegmatis Grown in Static Culture. C. RATLEDGE AND M. J. HALL. . . 314

Antibacterial Nitroacridine, Nitroakridin 3582: Effects on Bacterial Growth and Macro-molecular Biosynthesis In Vivo. ALAN D. WOLFE, THOMAS M. COOK, ANDFRED E. HAHN ......................................................... 320

Utilization of Glucose and the Effect of Organic Compounds on the ChemolithotrophThiobacillusferrooxidans. ROBERT TABITA AND D. G. LUNDGREN....... 328

Heterotrophic Metabolism of the Chemolithotroph Thiobacillusferrooxidans. ROBERTTABITA AND D. G. LUNDGREN............................................ 334

Transfer Ribonucleic Acid Methylases During the Germination of Neurospora crassa.ROSEMARY S. L. WONG, GENE A. SCARBOROUGH, AND ERNEST BOREK........... 446

Cold Osmotic Shock in Saccharomyces cerevisiae. J. W. PATCHING AND A. H. ROSE. . 451Microbial Transformation of Antibiotics: Phosphorylation of Clindamycin by Strepto-

myces coelicolor Muller. JOHN H. COATS AND ALEXANDER D. ARGOUDELIS ... . 459Isolation and Fractionation of the Photosynthetic Membranous Organelles from

Rhodopseudomonas spheroides. PAMELA J. FRAKER AND SAMUEL KAPLAN.465

XXiCONTENTS

Antimicrobial Actions of Hexachlorophene: Cytological Manifestations. J. N.SILVERNALE, H. L. JOSWICK, THOMAS R. CORNER, AND PHILIPP GERHARDT....... 482

Antimicrobial Actions of Hexachlorophene: Release of Cytoplasmic Materials. H.L. JOSWICK, THOMAS R. CORNER, J. N. SILVERNALE, AND PHILIPP GERHARDT...... 492

Antimicrobial Actions of Hexachlorophene: Lysis and Fixation of Bacterial Proto-plasts. THOMAS R. CORNER, H. L. JOSWICK, J. N. SILVERNALE, AND PHILIPPGERHARDT... ... ... ... . ..s.. ......... .............. ....... 501

Bifasic Growth of Acetobacter suboxydans on a Glycerol-Limiting Medium. B. L.BATZING AND G. W. CLAUS............................ ...................592

Chemotactic Response to Amino Acids by Pseudomonas aeruginosa in a SemisolidNitrate Medium. P. B. HAMILTON AND GAIL SHELLEY....................... 596

Analysis of a Model for Multiseptation in Bacteria. I. RICHARD LAPIDUS........... 607Extracellular Lipid of Thiobacillus thiooxidans. JAMES L. BEEBE AND W. W. UMBREIT. 612

EnzymologyCharacterization of a Thermolabile Sulfite Reductase from Salmonella pullorum.

WALTER D. HOEKSEMA AND DELBERT E. SCHOENHARD........................ 154#-Lactamases of R Factors Derived from Shigella and Salmonella Strains. PHILIP G.

JENKINS AND WILLIAM T. DRABBLE......................................... 159Purification and Properties of a Polygalacturonic Acid Trans-Eliminase Produced by

Bacillus pumilus. BHALCHANDRA A. DAVE AND REESE H. VAUGHN............ 166Pseudomonas putida Tryptophan Synthetase: Partial Sequence of the a Subunit.

IRVING P. CRAWFORD AND CHARLES YANOFSKY...................................248Growth of Aerobacter aerogenes on D-Arabinose: Origin of the Enzyme Activities.

EUGENE J. OLIVER AND ROBERT P. MORTLOCK.............................. 287Metabolism of D-Arabinose by Aerobacter aerogenes: Purification of the Isomerase.

EUGENE J. OLIVER AND ROBERT P. MORTLOCK...................... 293Glucose-6-Phosphate Dehydrogenase from the Chemolithotroph Thiobacillus ferro-

oxidans. ROBERT TABITA AND D. G. LUNDGREN............................ 343Pseudomonas putida Tryptophan Synthetase. TosHIo ENATSU AND I. P. CRAWFORD. . 431Involvement of Sulfhydryl Groups in the fj-Galactosidase of Streptococcus lactis.

G. A. MCFETERS, W. E. SANDINE, AND P. R. ELLIKER........................ 599Inhibition of Ribonuclease II of Escherichia coli by Sodium Ions, Adenosine-5'-Tri-

phosphate, and Transfer Ribonucleic Acid. P. VENKOV, D. SCHLESSINGER, ANDD. LONGO. . . . . . . . . . . . . . . . . . . . ......................... ... 601

Extracellular Nuclease in the Genus Lactobacillus. A. MILLER III, W. E. SANDINE, ANDAND P. R. ELLIKER...................................................... 604

Volume 108 Contents for November Number 2

Taxonomy and Ecology

Comparative Zone Electrophoresis of Enzymes of Pseudomonas solanacearum andPseudomonas cepacia. JAMES N. BAPTIST, CHARLES R. SHAW, AND MANLEYMANDEL............................................................... 799

Morphology and Ultrastructure

Structural Changes During Lysis of a Psychrophilic Marine Bacterium. J. Y. D'AousTAND D. J. KUSHNER.............. 916

Scanning Electron Microscopy of Intact Trichoderma Colonies.ESRA GALUN.938Amino Acids of the Cell Wall of Nocardia rubra. B. L. BEAMAN, K. S. KIM, M. R. J.

SALTON, AND L. BARKSDALE .............................................. 941

xxii CONTENTS

Genetics and Molecular BiologyPhosphoglucomutase Mutants of Escherichia coli K-12. SANDAR ADHYA AND MAXIMESCHWARTZ.............. . . . .. ........ 621

Characterization of Mutants of Escherichia coli Temperature-Sensitive for RibonucleicAcid Regulation: an Unusual Phenotype Associated with a Phenylalanyl TransferRibonucleic Acid Synthetase Mutant. ALAN G. ATHERLY AND MARTHA C.SUCHANEK.............................................................. 627

Potassium Transport Loci in Escherichia coli K-12. WOLFGANG EPSTEIN AND BYUNG S.KiM................. 639

Mapping the Gene Determining Ornithine Transcarbamylase and Its Operator inEscherichia coli B. GEORGE A. JACOBY ..................................... 645

Macromolecular Synthesis in Bacillus subtilis During Development of the CompetentState. DOUGLAS C. DOOLEY, CHARLES T. HADDEN, AND EUGENE W. NESTER...... 668

Fate of Transforming Deoxyribonucleate in Bacillus subtilis. MIROSLAWA PIECHOWSKAAND MAURICE S. Fox.................................................... 680

Biochemical Characterization of the ctr Mutants of Escherichia coli. HELVISE G. MORSE,WILLIAM K. PENBERTHY, AND M. L. MORSE................................. 690

Extent of Host Deletions Associated with Bacteriophage P2-Mediated Eduction.MELVIN G. SUNSHINE AND BEATRICE KELLY................................ 695

Synthesis and Some Properties of an Actin-Like Nuclear Protein in the Slime MoldPhysarum polycephalum. BRIGITTE M. JOCKUSCH, DONNA F. BROWN, AND HAROLDP. RUSCH ..... ...................................................... 705

Effect of Hydroxylysine on the Biosynthesis of Lysine in Saccharomyces. ASRU K.SINHA, MARK KURTZ, AND JNANENDRA K. BHATTACHARJEE ................... 715

Mechanism of Action of Rifampin on Mycobacterium smegmatis. R. J. WHITE, G. C.LANCINI, AND L. G. SILVESTRI....... 737

Isolation and Partial Characterization of Temperature-Sensitive Escherichia coli Mu-tants with Altered Leucyl- and Seryl-Transfer Ribonucleic Acid Synthetases. B.Low, F. GATES, T. GOLDSTEIN, AND D. SOLL................................ 742

Mutants of Escherichia coli Unable to Make Protein at 42 C. R. R. B. RUSSELL ANDA. J. PITTARD ................. 790

Stimulation of Ribonucleic Acid Syntesis by hloramphenicol in a rel+ Aminoacyl-Transfer Ribonucleic Acid Synthetase Mutant of Escherichia coli. CHARLES D.YEGIAN AND REBECCA W. VANDERSLICE .................................... 849

Phenotypic Restoration by Molybdate of Nitrate Reductase Activity in chlD Mutantsof Escherichia coli. J. H. GLASER AND J. A. DEMoss ................ 854

Analysis for Antigenic Similarities Among the 30S Ribosomal Proteins of Escherichiacoli. BRUCE C. STRNAD AND PAUL S. SYPHERD .................. 910

RecA+-Dependent Repair of Gamma-Ray Damage to Escherichia coli Does NotRequire Recombination Between Existing Homologous Chromosomes. B. A.BRIDGES................................................................ 944

Relationship Between Sex-Pili Formation and Macarbomycin Sensitivity in Escherichiacoli. SHIZUKO IYOBE, SUSUMU MITSUHASHI, AND HAMAO UMEZAWA . ........ 946

Evidence That the Majority of Leucine Transfer Ribonucleic Acid Is Not Involved inRepression in Salmonella typhimurium. MARTIN FREUNDLICH, JOHN TRELA, ANDWILLOW PENG .......................................................... 951

Physiology and MetabolismChanges in Terminal Respiratory Pathways of Bacillus subtilis During Germination,

Outgrowth, and Vegetative Growth. KUNIO TOCHIKUBO...................... 652Binding of Radioactive Benzylpenicillin to Sporulating Bacillus Cultures: Chemistry

and Fluctuations in Specific Binding Capacity. PAUL J. LAWRENCE, MARVINROGOLSKY, AND Vo THI HANH ............................................ 662

Acrylamide Gel Electrophoresis of Intracellular Proteins During Early Stages ofSporulation in Bacillus subtilis. KENNETH F. BoTr ........................ 720

Denitrification with Methanol: a Selective Enrichment for Hyphomicrobium Species.GEORGE T. SPERL AND DEREK S. HOARE.................................... 733

CONTENTS xxiii

Phosphorolysis of 5-Fluoro-2'-Deoxyuridine in Escherichia coli and Its Inhibition byNucleosides. EZRA YAGIL AND ARIE ROSNER................................ 760

Pigmentation and Acriflavine Resistance in Serratia marcescens. D. R. Woods, T. R.MOSMANN, SALLY HANSON, AND D. A. HENDRY............................. 765

Total Synthesis of Acetate from C02 Methyltetrahydrofolate, an Intermediate, and aProcedure for Separation of the Folates. DONALD J. PARKER, TSAI-FENG Wu, ANDHARLAND G. WOOD ......................................................770

Fatty Acid Composition of Cladosporium resinae Grown on Glucose and on Hydro-carbons. J. J. COONEY AND C. M. PROBY................................... 777

Metabolism of Poly-,B-Hydroxybutyrate: Effect of Mild Alkaline Extraction on NativePoly-,B-Hydroxybutyrate Granules. R. J. GREBEL AND J. M. MERRICK ....... . 782

'y-Aminobutyric Acid as a Required Germinant for Mutant Spores of Bacillus mega-terium. HAROLD F. FOERSTER ............................................ 817

Electrophoretic Heterogeneity of Bacterial Nicotinamide Adenine DinucleotidePhosphate-Specific Isocitrate Dehydrogenases. JOHN J. RowE AND HENRY C.REEVES.................................. ...............824

Physiology of Sporeforming Bacteria Associated with Insects. IV. Glucose Catabolismin Bacillus larvae. GRANT ST. JULIAN AND LEE A. BULLA, JR................... 828

Effects of Hydrostatic Pressure and Temperature on the Uptake and Respiration ofAmino Acids by a Facultatively Psychrophilic Marine Bacterium. KALA L. PAULAND RICHARD Y. MORITA ................................................ 835

Effects of Various Drugs on Hemolytic Activity of Streptococcus zymogenes. JEAN M.WERTH AND ROBERT W. JACKSON.......................................... 844

Fatty Acid Composition of Bifidobacterium and Lactobacillus Strains. J. H. VEERKAMP.. 861Macromolecular Sieving by the Dormant Spore of Bacillus cereus. RENE SCHERRER, T.

CABRERA BEAMAN, AND PHILIPP GERHARDT................................. 868Chemical Characterization of a New Surface Antigenic Polysaccharide from a Mutant

of Staphylococcus aureus. TERESA C. M. Wu AND JAMES T. PARK.............. 874Growth of Escherichia coli on Short-Chain Fatty Acids: Growth Characteristics of

Mutants. JOSEPH P. SALANITRO AND WARNER S. WEGENER.................... 885Growth of Escherichia coli on Short-Chain Fatty Acids: Nature of the Uptake System.

JOSEPH P. SALANITRO AND WARNER S. WEGENER.......... i_................. 893Distribution of the Sites of Alkaline Phosphatase(s) Activity in Vegetative Cells of

Bacillus subtilis. B. K. GHOSH, J. T. M. WOUTERS, AND J. 0. LAMPEN ....... . 928Nutritional Requirements of Arizona, Citrobacter, and Providencia. NEAL A.

MACHTIGER AND WILLIAM M. O'LEARY .................................... 948Nucleotide Pools in Growing Chromatium Strain D. MARGARET L. MIOvIC AND

JANE GIBSON............................................................ 954

EnzymologyOxidation of Nicotinic Acid by a Bacillus Species: Purification and Properties of

Nicotinic Acid and 6-Hydroxynicotinic Acid Hydroxylases. RONA HIRSCHBERGAND J. C. ENSIGN ................................................. i...... 751

Oxidation of Nicotinic Acid by a Bacillus Species: Source of Oxygen Atoms for theHydroxylation of Nicotinic Acid and 6-Hydroxynicotinic Acid. RONA HIRSCHBERGAND J. C. ENSIGN ....................................................... 757

Purification and Characterization of the L Component of Streptococcus zymogenesLysin. PAUL A. GRANATO AND ROBERT W. JACKSON......................... 804

Amino Acid-,B-Napthylamide Hydrolysis by Pseudomonas aeruginosa Arylamidase.P. S. RILEY AND FRANCIS J. BEHAL..................... I I ................ 809

Evidence for Distinct Kynureninase and Hydroxykynureninase Activities in Neuro-spora crassa. F. H. GAERTNER, K. W. COLE, AND G. R. WELCH............... 902

XXIV CONTENTS

CONTENTS ~~~~~~~xxvVolume 108 Contents for December Numnber 3

Taxonomy and EcologyTaxonomy of Marine Bacteria: Beneckea nigrapulchrituda sp. n. PAUL BAUMANN,

LINDA BAUMANN, M. MANDEL, AND RICHARD D. ALLEN.1380

Morphology and Ultrastructure

Spore Appendages and Taxonomy of Clostridium sordelli. L. J. RODE, LEODOCIA POPE,CAMILLE FILIP, AND LOUIS DS. SMITH.................................... 1384

Giant Cells of Escherichia coli: a Morphological Study. DAVID P. ALLISON.......... 1390Effect of Polymyxin on the Bacteriophage Receptors of the Cell Walls of Gram-Nega-

tive Bacteria. MASAATSU KOIKE AND KYOKO IIDA........................... 1402Membranous Structure of Escherichia coli Lipopoylsaccharide. YUTAKA KATAYAMA,

HIROSHI HATTORI, AND ATSUSHI SUGANUMA ............. 1412Lack of Distinction Between Nitrobacter agilis and Nitrobacter winogradskyi. PAULINE

H. C. PAN............................................................. 1416

Genetics and Molecular BiologyTumor Induction by Agrobacteriwn tumefaciens: Specific Transfer of Bacterial Deoxy-

ribonucleic Acid to Plant Tissue. DAVID M. YAJKO AND GEORGE D. HEGEMAN. . 973Resistance to Penicillin in Mutants of a Penicillinase-Negative Organism, Staphylococ-

cus aureus H. JAMES T. PARK, MARY E. GRIFFITH, AND IAN STEVENSON........ 1154Fertility of Salmonella typhimurium Crosses with Escherichia coli. T. MOJICA-A AND

R. B. MIDDLETON ..................................................... 1161Methionineless Death in Escherichia coli. T. R. BREITMAN, A. FINKLEMAN, AND

M. RABINOVITZ............................... 1168Enzyme Alterations in Tyrosine and Phenylalanine Auxotrophs of Salmonella typhi-

murium. J. DAYAN AND D. B. SPRINSON.................................. 1174Influences of Various Amino Acids on Tryptophan-Mediated Control of the Trypto-

phan Biosynthetic Enzymes in Escherichia coli. JOHN D. STUBBS AND E. ANNSTUBBS... . ..... .. . . ..1181STUBBS ~~. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ..............18

Effects of the Recipient Strain and Ultraviolet Irradiation on Transduction Kineticsof the Penicillinase Plasmid of Staphylococcus aureus. SALLY J. RUBIN AND E. D.ROSENBLUM......................................... ................... 1192

Effects of Ethidium Bromide on Growth and on Loss of the Penicillinase Plasmid ofStaphylococcus aureus. SALLY J. RUBIN AND E. D. ROSENBLUM .............. 1200

Developmental Regulation of Amylase Activity During Fruiting of Schizophyllumcommune. MARVIN N. SCHWALB .......................................... 1205

Resistance of Escherichia coli to Penicillins. IX. Genetics and Physiology of Class IIAmpicillin-Resistant Mutants That Are Galactose Negative, or Sensitive toBacteriophage C21, or Both. KERSTIN G. ERIKSSON-GRENNBERG, KURT NORD-STROM, AND PER ENGLUND................................................ 1210

Anaerobic L-a-Glycerophosphate Dehydrogenase of Escherichia coli: Its GeneticLocus and Its Physiological Role. W. S. KISTLER AND E. C. C. LIN............ 1224

Properties of an R Factor from Pseudomonas aeruginosa. NAOMI DATTA, R. W.HEDGES, ELIZABETH J. SHAW, R. B. SYKES, AND M. H. RICHMOND............ 1244

Evidence Suggestive of Compartmentalization of Deoxyribonucleic Acid-SynthesizingSystems in Freeze-Treated Bacillus subtilis. DANIEL BILLEN, LAURA B. CARREIRA,CHARLES T. HADDEN, AND SAUL J. SILVERSTEIN............................ 1250

Regulation of the Mandelate Pathway in Pseudomonas aeruginosa. S. L. ROSENBERG. . 1257Genetics of the Mandelate Pathway in Pseudomonas aeruginosa. S. L. ROSENBERG AND

G. D. HEGEMAN...................................... 1270Bacterial Cell Division Regulation: Physiological Effects of Crystal Violet on Esche-

richia coli Ion+ and lon- Strains. JAMEs R. WALKER, NAWAL A. SHAFIQ, ANDROBERT G. ALLEN ...................................................... 1296

CONTENTS xxv

Genetic Analysis of an Escherichia coli Syndrome. EVELYNE T. LENNETTE AND DAVIDAPIRION..................................... .......1322

Isolation of Circular Deoxyribonucleic Acid from Salmonella typhosa Hybrids Ob-tained from Matings with Escherichia coli Hfr Donors. R. W. LEAVITT, J. A.WOHLHIETER, E. M. JOHNSON, G. E. OLSON, AND L. S. BARON............. 1357

Division Mutants of Bacillus subtilis: Isolation and PBS1 Transduction of Division-Specific Markers. D. VAN ALSTYNE AND M. I. SIMON......................... 1366

Alkaline Sucrose Gradient Sedimentation of Chromosomal Deoxyribonucleic Acidfrom Escherichia coli PolA+ and PolA- Strains During Thymine Starvation.STEVEN G. SEDGWICK AND BRYN A. BRIDGES.............................. 1422

Genetic Determinant in Escherichia coli Affecting Thymineless Death and UltravioletSensitivity. A. WILKINS, J. GALLANT, AND B. HARADA........................ 1424

Direct Selection for Transduction of Suppressor Mutations and Linkage of supD tofla Genes in Salmonella. DAVID B. FANKHAUSER AND PHILIP E. HARTMAN...... 1427

Recognition of an Escherichia Operator by a Salmonella Repressor. MARJORIE C.BRANDRISS AND J. M. CALVO............................................ 1431

Physiology and Metabolism

Study of Adenine Aminohydrolase in the Yeast, Schizosaccharomyces pombe. ANGELOABBONDANDOLO, ANNE WEYER, HENRI HESLOT, AND MICHELINE LAMBERT..... 959

Restoration of Deoxycholate-Disrupted Membrane Oxidases of Micrococcus lysodeik-ticus. R. C. EISENBERG ........................ 964

Incorporation of Radioactive Macromolecular Precursors into Intact Cells and Os-motically Stabilized "Protoplasts" of Streptococcus faecalis. GEORGE S. ROTHAND LOLITA DANEO-MOORE .............................................. 980

Biosynthesis of Cholesteryl Glucoside by Mycoplasma gallinarum. PAUL F. SMITH... 986Fatty Acids of Thiobacillus thiooxidans. RICHARD A. LEVIN .............. 992Caffeic Acid Metabolism by Bacteria of the Human Gastrointestinal Tract. MARK A.

PEPPERCORN AND PETER GOLDMAN ........... 996Role of Vitamin B6 Biosynthetic Rate in the Study of Vitamin B6 Synthesis in Escherichia

coli. WALTER B. DEMPSEY ............................................1001Oxidative Phosphorylation in Fractionated Bacterial Systems: Effect of Chloram-

phenicol. BEN-ZION CAVARI, VIJAY K. KALRA, AND ARNOLD F. BRODIE........ 1017Antibacterial Nitroacridine, Nitroakridin 3582: Binding to Nucleic Acids In Vitro and

Effects on Selected Cell-Free Model Systems of Macromolecular Biosynthesis.ALAN D. WOLFE, THOMAS M. COOK, AND FRED E. HAHN . ................. 1026

Escherichia coli K-12 Mutants Altered in the Transport of Branched-Chain AminoAcids. JOHN GUARDIOLA AND MAURIZIO IACCARINO ........................ 1034

Use of Yeast Populations Fractionated by Zonal Centrifugation to Study the CellCycle. J. SEBASTIAN, B. L. A. CARTER, AND H. 0. HALVORSON ............. 1045

Activation of Clostridium botulinum Type B Toxin by an Endogenous Enzyme. BIBHUTIR . DASGUPTA.......................................................... 1051

Detection of a Rapidly Metabolizing Portion of the Membrane Cardiolipin in Haemo-philus parainfluenzae. ANNE N. TUCKER AND DAVID C. WHITE............... 1058

Consequences of the Inhibition of Cardiolipin Metabolism in Haemophilus para-influenzae. YOSHIE ONO AND DAVID C. WHITE............................. 1065

Adenylate Energy Charge in Escherichia coli During Growth and Starvation. ASTRID G.CHAPMAN, LANA FALL, AND DANIEL E. ATKINSON......................... 1072

Cyanide-Resistant Respiration in Neurospora crassa. ALAN M. LAMBOWITZ ANDCAROLYN W . SLAYMAN .................................................. 1087

Biosynthesis of 7,8-Diaminopelargonic Acid, a Biotin Intermediate, from 7-Keto-8-Aminopelargonic Acid and S-Adenosyl-L-Methionine. MAX A. EISENBERG ANDGERALD L. STONER.................................................... 1135

Structural Effects on Arthrobacter Methylene Hydroxylase Activity. STEVENHAYASAKA AND D. A. KLEIN............................................. 1141

Role of Autolysins in the Killing of Bacteria by Some Bactericidal Antibiotics. H. J.ROGERS AND C. W. FORSBERG........................................... 1235

XXVI CONTENTS