IOM3 9.11. 2009

Ultra thin ,prophylactic, “Liquid Glass” coatings (SiO2) for use in Health Care and associated

environments.

Neil McClelland

UK Project Manager

Nanopool GmbH

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Key areas covered in this presentation

• Description of the technology

• Test data associated with the technology

• Areas of application

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• Nanopool are currently regarded as world leaders in the field of Si02 ultra thin layering technology.

• We supply our multi award winning technology to a very wide range of markets, including the retail sector.

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IntroductionIntroduction

• NP coatings are nano scale ( 80-100nm +/-) but do not contain discrete or engineered nano particles.

• A nanometre is equivalent to a millionth part of a millimetre = 10-9 m

• A human hair is approximately 50,000 nm thick, therefore our coatings are approximately 500 times thinner than a human hair.

So what is in the bottle?

Molecules of SiO2 and water Molecules of SiO2 and ethanol

• SiO2 is a well understood in the macro world . It consumed daily by humans and whales!

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NP-Nano LayersNP-Nano Layers

• By applying a modified SolGel-process we generate ultra thin SiO2-coatings

• SiO2 is pristine glass. One of its really exciting characteristics is an extremely low surface tension

• It is very hydrophobic and simultaneously oleophobic. (We use the term super-phobic.)

Substrate

Organized, web-structured

SiO2-molecules

np-nano coating

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•The coatings are easy to apply via -wiping, spraying or dipping.•Re application is very simple.

•The coatings can be applied to almost any surface, including skin. All coatings are breathable (allow diffusion).

•Super-phobicity ensures stain protection. Soiling agents are unable to adhere to the surface.

•The coatings are highly durable, lasting years in many instances. Our coatings will withstand tens of thousands of wiping actions

•All coated surfaces can, in most instances, be cleaned with water alone. (This observation is supported by a range of test data.)

Some further characteristics of nanopool’s easy to clean protective coatings

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NP-Nano LayersNP-Nano Layers

• The SiO2 skin is enormously flexible, acid and alkaline resistant and withstands extreme temperature changes -150 to +450C

• Resistant to Acid and Alkali

• ph-Value 0.5 – 12 (14 depending on type of substrate)

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•All of Nanopool’s coatings are “cationic” and therefore provide biostatic characteristics.

•We also offer an Anti Bacterial range of coatings which contain triclosan within the matrix. These products conform to ASTMs e 2180

Fabric Treatment11

• This image shows the coating on a micro fibre. (The fissure was induced.)The corrugated appearance indicates the flexibility of the coating

AFM Images showing topography of coated surface12

Uncoated surface scale 2.0µm

Coated surface . Scale 1 µm

Glass substrate

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NP-Nano LayersNP-Nano Layers Ultra thin Layer on a metal substrateUltra thin Layer on a metal substrate

Flexibility

• coated polypropylene . 90 degree bend indicating flexibility

• Our coatings are flexible ( 200%)

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Antimicrobial CharacteristicsAntimicrobial Characteristics

• The hydrophobic and oleophobic function of the SiO2-coating impedes the colonization of surfaces

• The strongly cationic characteristic of the SiO2-Triclosan (or Chitosan under development )-Matrix results in enormous weakening of the cell walls through ion exchange reactions.

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Antimicrobial CharacteristicsAntimicrobial Characteristics

• The cytoplasm and its membrane are encapsulated in an inflexible cell wall.

• Bacteria are classified into two different categories – gram-positive and gram-negative.

• Gram-positive bacteria have a thick, simple cell wall structure (20-80 nm), consisting of peptidoglycan; opposed to this ,gram-negative bacteria have a thin but complex cell wall structure consisting of an outer membrane (7,5-10 nm) and underneath a thin layer of peptidoglycan (5-10 nm).

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Antimicrobial CharacteristicsAntimicrobial Characteristics

• The cationic ( pos. charged) “donating-agent” is absorbed on the negative charged surface of the bacteria.

• Then it diffuses through the cell wall• It bonds to the cytoplasm membrane. • This causes the decomposition of the

cytoplasm membrane. • This leads to the disintegration of the

cytoplasm components. • The cell dies. Ref ASTM e2180

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Antimicrobial CharacteristicsAntimicrobial Characteristics

• Contributes to Hygiene and Cost Savings – Easy-to-Clean-characteristic = easier

and faster cleaning (audited figures suggest an overall saving of 35%)

– Actively and constantly reduces the microbial level

– Prevents or inhibits microbial growth – Has a bactericidal, fungicidal and

algaecide function

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Certificates and ExpertisesCertificates and Expertises

                                     

                     

                                     

                     

                                 

                     

                  

anti bacterial effect -fabric coated in SIO220

This image shows the effect on Staphylococcus aureus bacteria around a sample fabric coated with NP Fabric Protect anti-bac (right) and a sample coated with NP 12 anti-bac variant(left), after a 24 hour incubation at 36°C.

“As a result it can be stated that there is a diffusion of antibacterially effective compounds on the coated samples”

Hemmhof protocol - conducted by the IBFE organisation

PD Dr. Thomas Jahns /Dr.Heiko Ewen

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Food safety.

• Ultra thin layers generated with nanopool products are according to EU 1935/2004 applicable in food and beverage production processes

EU VO 1935/2004EU VO 1935/2004Certificates and ExpertisesCertificates and Expertises

ASTM s E 2180

• Quantitative definition of the antimicrobial effects

• according to ASTM Standard E2180

• Institut Fresenius

• Product: np liquid glass antibac

• Sample denomination: ceramic disc covered with np liquid glass antibac

• Incubation tempertaure: bacteria 36°C ± 2°C

• Expert assessment:

• “The product has significant, strong bactericide effects on the implemented testing germ( after 5 days residence time) Escherichia Coli, Staphylococcus aureus and Salmonella aeruginosa.”

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EN 1040 23

The results show living organism number after 60 minutes 0/0“ Reduction of living germ number > 105””

Anti microbial effect of “standard” coatings

Cooking pot treated with “Die Nanoexperten – Cooking pot and oven protect”

Bacteria re-extracted by adhesive film tests ( each in parallel) of a treated and untreated cooking pot

• coated uncoated• T0 260/244 284/302• T1 154/140 225/222

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Nanopool GmbH provided two cooking pot samples (one untreated, one treated with“Die Nanoexperten – pot and oven protect”). The pots were tested for antimicrobial effectiveness by being contaminated artificially with a suspensionof Staphylococcus aureus , residual bacteria were recovered from the surfaces by adhesive film tests

The tests for the antimicrobial effectiveness have been carried out at roomtemperature (23°C). 100 ml of the bacteria suspension was placed in eachcooking pot (germ density about 2,8 x 106/ml) and was incubated for 5 min. withgentle shaking. Afterwards the pots were cleared and after drying of the surfaceat 36°C two adhesive film tests on yeast extract-peptone-media (YEP) -taken (point of time T0). Then the pots have been rinsed out each one time with100ml de-ionised water (5 min. with gentle shaking) and again cleared.After drying of the surfaces two adhesive film tests were taken once more onthe yeast extract-peptone-media (point of time T1). Table 1 shows the values gainedat the respective points of time for the re-extracted germs of the treated resp.untreated pot.

Issues

• Preparation

• Applying in a busy working environment

• Cleaning regimes.

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Preparation

• Almost all surfaces can be coated with great ease but…

• the surface to which the coating is applied has to be completely clean. A fingerprint can be 100 times thicker than the coating.

• Application to surgical devices and stand alone equipment is straight forward.

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Uses within Healthcare27

• The potential uses within Health Care are almost endless. The following images are from Health care environments

Cleaning equipment with water 28

The left hand side of this equipment is coated with SiO2 and it is cleaned daily with water. The right hand side is not coated ; it is also cleaned daily with water.

On going trials 29

General medical instruments

Stents

Endoscopes -SiO2 coatings inhibit the adhesion of prions

Suture materials coated to inhibit infection

Clothing and special fabrics

Application within “Live” working environments

• Coating surfaces within a busy hospital presents issues re cleaning and post application drying times for thicker coatings. EG flooring/fabrics.

• Thinner coatings can be handled in 2-3 minutes.

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Cleaning Regimes

• The SiO2 coatings can be cleaned efficiently with warm water. Test data available

• Many cleaning products deposit surfactants and residues on the coating and thus limit the efficacy of the coating.

• Coated surfaces can be rejuvenated after prolonged use by wiping with alcohol.

• We suggest that NP coatings are used as part of a cleaning and disinfection regime. We do not suggest that disinfection is no longer required in Healthcare environments after surfaces have been coated with SiO2.

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SummarySummary

• There are only a few companies world-wide which are involved in developing this form of technology . Of these few only nanopool offers a full range of finished products

• Our products are certified as being environmentally friendly , and physiologically harmless.

• This is a “low cost high benefit” technology.

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Thank you very Thank you very much for your much for your

attention!attention!