Integrated ScienceIntegrated Science Day 8 Day 8

September 24, 2012September 24, 2012

Learning targetsLearning targets

1.1. I can evaluate a laboratory procedure I can evaluate a laboratory procedure and analyze the data to form a and analyze the data to form a conclusionconclusion

2.2. I can Set up an Inquiry lab by coming up I can Set up an Inquiry lab by coming up with an investigative question and with an investigative question and determining my needs for the lab. determining my needs for the lab.

AgendaAgenda

Kick off question: Kick off question:

What ideas do you have for your science fair What ideas do you have for your science fair project?project?1.1.Heart rate lab conclusion should be turned in for a Heart rate lab conclusion should be turned in for a gradegrade

2.2.Daphnia Lab conclusion turned in todayDaphnia Lab conclusion turned in today

3.3.Set up for the effect of substances on organisms in Set up for the effect of substances on organisms in our waterwaysour waterways

4.4.Learning log: Learning log: What is a bioassayWhat is a bioassay??

Water ContaminationWater Contamination

How do we know what is “clean water?” In How do we know what is “clean water?” In other words, how do we know if water is other words, how do we know if water is “safe” for humans and wildlife?“safe” for humans and wildlife?

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Bioassays- notesBioassays- notes

Dose/Response Bioassays are used to:Dose/Response Bioassays are used to: Estimate toxicity to humans.Estimate toxicity to humans. Estimate maximum concentrations of specific Estimate maximum concentrations of specific

chemicals allowed to discharge into bodies of chemicals allowed to discharge into bodies of water.water.

Investigate hazardous waste sites. Investigate hazardous waste sites.

Bioassay

A bioassay involves the use of biological organism to test for chemical toxicity.

Canaries are used by coal miners as a way to test for high levels of methane (an odorless gas)

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Bioassay SpeciesBioassay Species

3 organisms are often used3 organisms are often used DaphniaDaphnia DuckweedDuckweed Lettuce SeedsLettuce Seeds

Expose organisms to chemicals and Expose organisms to chemicals and measure how they respond.measure how they respond.

Used by the FDA

Bioassays are used to test drugs and skin care products before being “okayed” by the FDA.

DaphniaDaphnia

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CCONCENTRATION VS. DOSEONCENTRATION VS. DOSE

LDLD5050 the the DDose that is ose that is LLethal for 50% of the ethal for 50% of the

test organisms. test organisms. Used for DaphniaUsed for Daphnia

TCTC50 50 the the CConcentration that causes a 50% oncentration that causes a 50%

drop in growth or health of the test drop in growth or health of the test organisms (organisms (TToxic oxic CConcentration).oncentration). Used for seeds (they may not die, just not Used for seeds (they may not die, just not

sprout) sprout)

An Introduction To Experimental DesignAn Introduction To Experimental Design

The The TreatmentsTreatments in an experiment in an experiment represent the factor that you vary while represent the factor that you vary while keeping everything else constant.keeping everything else constant.

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An Introduction To Experimental DesignAn Introduction To Experimental Design

The The control control is the untreated group, used is the untreated group, used for comparison with the treatment groups.for comparison with the treatment groups.

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An Introduction To Experimental DesignAn Introduction To Experimental Design

ReplicatesReplicates are groups of organisms are groups of organisms exposed to identical conditions.exposed to identical conditions. The more replicates the better.The more replicates the better.

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