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ation rates existed. Therefore the reconstructed human skin modelscan be regarded as generally useful on lipophilic compounds for invitro penetration studies.

doi:10.1016/j.toxlet.2010.03.491

Abstracts / Toxicology L

ative Methods (ECVAM) for pre-validation following successfulethod evaluation activities within Colipa. Furthermore, data from

hese and other test methods are being used to develop testingtrategies capable of characterizing skin sensitizer potency withouthe need for new animal test data.

oi:10.1016/j.toxlet.2010.03.488

201-034n vitro screening method for gastrotoxicity using mucin layer

. Ha 1, S.C. Park 2, H. Kwon 3

Department of Food and Nutrition, Seoul National University, Seoul,epublic of Korea, 2 Department of Electronical Engineering, Seoulational University, Seoul, Republic of Korea, 3 Department of Foodnd Nutrition, and Research Institute of Human Ecology, Seoulational University, Seoul, Republic of Korea

ost frequent customer complaint of health functional fooderived from plant is gastrointestinal problems such as vomit-

ng, dyspepsia, brash, hyperacidity, diarrhea or constipation whicheople tend to underestimate. Stomach is the first organ whichncounters the foreign materials for an extended time period.he in vitro screening method is needed to establish to predictastrotoxicity. Mucins protect the stomach against damage andutodigestion caused by foreign materials and acidic condition.ecause the established cell line cannot secrete the mucins, in vitroests are difficult to reflect in this actual gastric environment. As aool to early screening, the gastric model that can be applied acidicondition and mucin was devised. Mucin layer laid on the MKN-28ells, the human gastric adenocarcinoma cell line, extended sur-ival time from four minutes to two hours in an environment (pH2).transwell system was employed to examine the destruction of

he mucin protection inflicted by aspirin which is well known forts adverse effect on stomach. The low concentration of aspirinendered the mucin layer more permeable compared, which highoncentration made it less permeable. High concentration of aspirin10 mM) induced the aggregation of mucin layer, which was con-rmed by scanning electron microscopy (SEM), and the surfaceermeability decreased consequently. Meanwhile low concentra-ion of aspirin (0.5 mM) made large pores on the mucin gel layer.he conformation of mucins changed in true shortend the absorp-ion time in the gastric cell layer, in vitro. The mucin layer damageas the first step in a serious of gastric cell damage signals such as

FF, AKT and pAKT.

oi:10.1016/j.toxlet.2010.03.489

201-035alidation of porcine corneal opacity and permeability assay asn alternative to eye irritation tests

.H. Han 1, S.H. Seok 2, Y.R. Na 1, T.H. Kim 1, H. Jung 1, C.H. Lim 3,

.H. Che 4, J.H. Park 1

Department of Laboratory Animal Medicine, College of Veterinaryedicine, Seoul National University, Korea, 2 Institute for

xperimental Animals, College of Medicine, Seoul National University,orea, 3 National Institute of Toxicological Research, Seoul, Korea,

Department of Experimental Animal Research, Clinical Research

nstitute, Seoul National University Hospital, Seoul, Korea

he Draize eye irritation test has been changed to alternative meth-ds based on 3 Rs (Replacement, Reduction, Refinement) of animal

196S (2010) S37–S351 S141

experiments. The bovine corneal opacity and permeability (BCOP)assay is well established as one of the alternative methods to theDraize test but the supply of bovine eyes from a slaughterhouse isnot sufficient in actuality. The porcine corneal opacity and perme-ability (PCOP) assay has been proposed as a reliable alternative tothe BCOP assay and the Draize test because porcine corneas havenumerous advantages including structural similarities with humancorneas and the stable capacity for a corneal supply. In this study,three cosmetics and seven chemicals that are already known asirritants at Draize score and the BCOP assay were tested by thePCOP assay for the first time in Korea. The porcine corneal opacitywas measured by quantifying the ability of light to pass througha cornea using an opacitometer. The permeability was measuredat 10 and 30 min time points after chemical exposure. The PCOPassay showed the high correlation with the Draize score and BCOPassay. These results showed that the PCOP assay can be used as analternative method for the BCOP assay and the Draize test althoughit needs further studies about insoluble chemicals and others toincrease reliability.

doi:10.1016/j.toxlet.2010.03.490

P201-036Comparison of reconstructed human skin models with humanand pig skin in in vitro percutaneous absorption

S.H. Seok 1, J.H. Han 2, Y.R. Na 2, T.H. Kim 2, H. Jung 2, C.H. Lim 3,J.H. Che 4, J.H. Park 1

1 Institute for Experimental Animals, College of Medicine, SeoulNational University, Korea, 2 Department of Laboratory AnimalMedicine, College of Veterinary Medicine, Seoul National University,Korea, 3 National Institute of Toxicological Research, Seoul, Korea,4 Department of Experimental Animal Research, Clinical ResearchInstitute, Seoul National University Hospital, Seoul, Korea

Percutaneous penetration studies are usually performed in humanskin samples and the ability to perform these studies may dependon the availability of skin samples. Reconstructed human skin mod-els have been proposed as alternatives for human skin to overcomesuch limitations. This study was carried out in accordance withOECD guidelines on skin absorption test for the comparison ofreconstructed human skin models, EFT-306 and KeraSkinFT (bothof reconstructed full-thickness skins) with human and pig skin.Three OECD standard compounds were used for penetration andabsorption: Caffeine, benzoic acid and testosterone, all of whichdiffer vastly in their lipophilicity. Reconstructed human skins weremore permeable in low lipophilic compound, caffeine comparedwith human and pig skin. Differences became most obvious withKeraSkinFT showing a relatively weak penetration barrier on caf-feine. EFT-306TM and KeraSkinFT both showed higher permeationrates in the case of lipophilic compounds, benzoic acid and testos-terone compared with human and pig skins. High correlation wasobserved on the penetration flux between the reconstructed humanskin models and human and pig skin, even though different perme-