identifying mu insertions in nutrient transport-related genes in the uniformmu maize population...
TRANSCRIPT
Identifying Mu Insertions in Nutrient Transport-related Genes in the UniformMu Maize Population
Overview:Research presented here tests the hypotheses that insertion of a Mu transposable element into any of the selected genes will cause a loss of function that results in a distinct mutant phenotype. The UniformMu maize population is a resource developed by introgressing the highly transposon-mutagenic line Robertson’s Mutator into the phenotypically-uniform inbred W22. This population provides the ability to identify and map Mu insertions, allowing researchers to select and characterize lines harboring mutations in genes of interest. Here we use both a forward and reverse genetics approach to identify insertions in genes potentially affecting nutrient transport. To this end, we attempt to associate insertions with mutant phenotypes through co-segregational analyses.
Stephanie M. Locke, Brent. A. O’Brien, Ryan Gannon, Johnathan Saunders, Laura Morales,
Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, FL, 32611
References: McCarty, D.R., Settles, A.M., Suzuki, M., Tan, B.C., and Latshaw, S. et al. (2005). Steady-state transposon mutagenesis in inbred maize. Plant J. 44: 52-61.
Localizes to the plasmodesmata and is predicted to bind callose, which is deposited at the sieve plate in response to injury and pathogen infection.GRMZM2G125241
Plasmodesmata callose binding protein 4 (PDCB4)
ABC (ATP binding cassette) transporters are transmembrane proteins that utilize the energy of ATP hydrolysis to pump substrate across the membrane against a concentration gradient.GRMZM2G156630
ABC-type transporter
Primarily functions in carbohydrate bindingIn A. Thaliana, this gene was found to be a N-terminal protein involved in myristoylation and control responses to phloem damage.GRMZM2G010855
Phloem protein 2A13 (PP2 A13)
This gene functions in carbohydrate transmembrane transporter activity.GRMZM2G160504GRMZM2G160460
Solute carrier family 2, facilitatedglucose transporter member 8 (SLC2A8)
Hydrolyzes sucrose to fructose and glucose in the cytoplasmGRMZM2G084940
Plant neutral invertase family protein
SCF23 represents integral membrane proteins that transport organic molecules and inorganic ions.GRMZM2G437859
Solute carrier family 23 member 2 (SCF23)
Reverse Genetics
Forward GeneticsFamily 011S-2189Phenotype = wilty
Insertions Tested
Segregation/genotypesInsertions Testedwith main phenotypes
Co-segregation
Mitochondrial transcription termination factor family protein
Long-chain acyl-CoA synthetase 7
Hercules receptor kinase 2
Protein of unknown function (DUF3537)
Peptidase M1 family protein
PapD-like superfamily protein
Sec-independent periplasmic protein translocase
Cellulose synthase-like D3
Succinate dehydrogenase 1-1
Segregation/genotypes KO with phenotypeCo-segregation of
Prominent Phenotypes
WT, Het, and Homozygous
WT, Het, and Homozygous
WT, Het, and Homozygous
WT, Het, and Homozygous
WT, Het, and HomozygousWT, Het, and Homozygous
WT, Het, and Homozygous
All WT
WT, and Homozygous
NO
NO
NO
NO
NO
NONO
NO
--
Empty pericarp (ep)Embryo lethal (emb)Leaf discoloration
Defective kernel (dek), Rough kernel (rgh), ep,
and emb
Small kernel (smk),ep, and emb
smk and emb
ep and dek
smk
WT, Het, and Homozygous
PCR inconclusive to date
All Het or WT (GRMZM2G160504)
All Het (GRMZM2G160460)
WT, Het, and Homozygous
All Het
WT, Het, and Homozygous
?
NO(other effects possible)
ep
smk Leaf discoloration
emb
Phenotype Examples
NO(other effects possible)
NO(other effects possible)
NO(other effects possible)
NO(other effects possible)
Wayne T. Avigne, Donald McCarty, Charles Hunter, and Karen E. Koch