hplc bulk property detectors refractive index detector
TRANSCRIPT
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HPLC Bulk Property Detectors
Refractive Index Detector
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Refractive Index Detector
Plus:1) Measures a bulk property2) Nearly Universal (different RI than mobile phase)3) Comparable response for different analytes4) Detects species with no chromophores
Minus:1) Temperature dependent2) Poor sensitivity (LOD ≈ 100 ng)3) No gradient elution
HPLC Bulk Property Detectors
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Evaporative Light Scattering Detector
Solid state laser
HPLC Bulk Property Detectors
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Plus:1) Measures a bulk property2) Nearly Universal (must be non-volatile)3) Does not detect liquids (gradients are ok)4) Detects species with no chromophores
Minus:1) Signal not linear with concentration2) Fair sensitivity (LOD ≈ 1 ng)3) No salts or buffers in mobile phase
Evaporative Light Scattering Detector
HPLC Bulk Property Detectors
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Conductivity Detector
HPLC Bulk Property Detectors
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Plus:1) Measures a bulk property2) Nearly Universal (must be ionic)3) Common for Ion Exchange LC4) Detects species with no chromophores5) Simple, robust
Minus:1) Fair sensitivity (LOD ≈ 1 ng)2) No salts or buffers in mobile phase3) Gradients a challenge
HPLC Bulk Property Detectors
Conductivity Detector
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HPLC Specific Property Detectors
Conventional UV-Vis Absorption Detector
Hg PenLamp
254 nm
Photodiode 2
Photodiode 1
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HPLC Specific Property Detectors
Multi-wavelength UV-Vis Absorption Detector
DeuteriumLamp
PhotodiodeArray
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HPLC Specific Property Detectors
Multi-wavelength UV-Vis Absorption Detector
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HPLC Specific Property Detectors
Multi-wavelength UV-Vis Absorption Detector
Plus:1) Measures a specific property2) Nearly Universal (must have chromophore)3) Most common of all detectors (~75%)4) Potential to provide qualitative info.5) Simple, robust
Minus:1) Fair sensitivity (LOD ≈ 1 ng)2) Expensive with PDA, limited with Hg Lamp3) Misses some important analytes
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HPLC Specific Property Detectors
Fluorescence Detector
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HPLC Specific Property Detectors
Fluorescence Detector
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HPLC Specific Property Detectors
Plus:1) Measures a specific property2) Highly Selective (must fluoresce)3) Second most common of all detectors (~15%)4) High sensitivity (LOD ≈ 0.01 ng)5) Can interrogate very small volumes
Minus:1) Not Universal2) Limited Applications
Fluorescence Detector
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HPLC Specific Property Detectors
Electrochemical Detector
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HPLC Specific Property Detectors
Plus:1) Measures a specific property2) Highly Selective (depends on reduction potential)3) High sensitivity (LOD ≈ 0.01 ng)
Minus:1) Not Universal2) Must have electrolyte in mobile phase3) Mobile phase must be aqueous4) Gradients not possible
Electrochemical Detector
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HPLC Element Specific Detectors
ICP Detector Detector
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Plus:1) Can be Highly Selective2) Can be nearly Universal3) Can have single sensitivity for all analytes
Minus:1) $$$2) Mobile phase must be aqueous4) Mobile phase must not contain element of interest
ICP Detector Detector
HPLC Element Specific Detectors
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UV-Vis detection of sugars in juice
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UV-Vis detection of sugars in juice
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ICP Carbon detection of sugars in juice
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ICP Carbon detection of sugars in orange juice
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ICP Carbon detection of amino acids
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ICP Carbon detection of amino acids
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Tag analytes with fluorophoreBefore or After Separation
HPLC Derivatization Detectors
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Tag analytes with fluorophoreBefore or After Separation
HPLC Derivatization Detectors
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Detect the change in a constant signalas the analyte passes
HPLC Indirect Detectors
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Hollow Cathode Detector
Recent HPLC Detector
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Hollow Cathode Detector
Recent HPLC Detector
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Hollow Cathode Detector
Recent HPLC Detector
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Hollow Cathode Detector
Recent HPLC Detector
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Hollow Cathode Detector
Recent HPLC Detector
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Hollow Cathode Detector
Recent HPLC Detector