global perspectives on trait ontology and phenotyping of ... · development of precision livestock...
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Global perspectives on trait ontology and phenotyping of
livestock: examples from functional genomics and modeling
in beef-producing animals
JF Hocquette1, C Capel2, M Barbezant3, PL Gastinel2, PY Le Bail1, P Monget1, JL Peyraud1
1 INRA, 2 Institut de l’Elevage, 3 UNCEIA
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Outline Some definitions and elements of context
Challenges about beef quality in genetics
Challenges about beef quality in functional genomics
Other perspectives
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Definitions : trait, phenotype, mesurement « Animal trait Ontology »
Trait
Phenotype Measurement
Single feature or quantifiable measurement
of an organism
What is measured to determine
status of trait
Status or value of a trait or an
organism relative to xxx
Ex: weight, tenderness
Ex: tenderness after cooking at 55°C or 74°C
Ex: fat - lean / though - tender
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High-throughput phenotyping
Measurement of phenotypes using a rapidand repeatable method that can beautomated so that the process generates alarge number of data• 2 components: systematic phenotyping (a few variables on many
animals) targeted or deep phenotyping (more variables for a
trait family on a small number of animals)
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One challenge: format and access of data.
Importance of ontologies
Data bases
ATOL Programme : Animal Trait Ontology of Livestock(see the presentation of Hurtaud et al)
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Output of such research
One overall objective is to have efficient, robust and adaptable animals in response to climatic variability
Robustness is the capacity of an animal to adapt to environmental challenges: it requires repeated and frequent measurements of phenotypes
Genotype-phenotype relationships Development of precision livestock farming
• To reduce feed costs and waste• To reduce labor load
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Outline Some definitions and elements of context
Challenges about beef quality in genetics
Challenges about beef quality in functional genomics
Other perspectives
7
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A challenge: the genomic selection
Growth
Fatness
Tenderness
Flavour
Association studies between genetic markers and phenotypes of interest (example : beef quality)
Example : the Qualvigène programme
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Breeding EnterprisesINRA LaboratoriesLivestock InstituteAbattoirsOtherProgeny Testing Stations
PépieuxDenguin
Marmilhat
Pont Ste Marie
UCHAVE
MIDATESTUALC
UCATRC
UCEF
SGQA
Unité GénétiqueMoléculaire Animale
Station deRecherchessur la Viande
Unité deRecherchessur les Herbivores
Service Sélection
Service Sélection
ServiceSélectionService Viande
Limoges
Pau
Villefranched'Allier
Migennes
CTIGLabogéna
UNCEIA
• Charolais, Limousin, Blond d’Aquitaine • 114 sires• 3349 young bulls
The French QUALVIGENE programme
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Material and Methods
Traits measured on the Longissimus thoracis muscle
Rib Sampling Ageing Measurements
7th 24 h 0 dayLipid and Collagen contentsMuscle Fibre Section area
8th 24 h 14 days Warner-Bratzler Shear Force*
9th 24 h 14 daysSensory attributes* :
Tenderness, Juiciness & Flavour scores* Cooking temperature = 55 °C
Measurement of phenotypes using a rapid (NO) and repeatable (YES)methods that can be automated (NO) so that the process generates a
large number of data (YES).
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European programme GEMQUAL (Genetics of Meat Quality)
Comparison of the same samples by two sensory panels in Spain and UK.
Calibration results for Beef FlavourUK + 1.7 = ES ScoreSpanish -1.7 = UK Score
Beefcal
Be
ef
fla
vo
ur
4.54.03.53.02.52.0
6.0
5.5
5.0
4.5
S 0.385530R-Sq 1.7%R-Sq(adj) 1.0%
Fitted Line PlotBeef flavour = 4.785 + 0.1135 Beefcal
The measurement of flavour is not repeatable
(Nute et al., 2006)
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European programme GEMQUAL
The measurement of tenderness is slightly repeatable
(Nute et al., 2006)
ES
UK
8765432
7
6
5
4
3
2
S 0.676257R-Sq 53.1%R-Sq(adj) 52.8%
Fitted Line PlotUK = 1.411 + 0.6187 ES
Calibration results for texture UK = 1.4 + 0.6 ES (n=206 paired values)
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Sensory Analysis at 55 °C and 74 °C
Sensory Analysis at 55 °C and 74 °C of 33 animals in a French laboratory
Tenderness at 55 °C
3,00
3,50
4,00
4,50
5,00
5,50
6,00
6,50
7,00
3,00 3,50 4,00 4,50 5,00 5,50 6,00 6,50 7,00
Tend
erne
ss a
t 74
°C
R = 0,46
Micol et al., 2011. EAAP
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Does imprecision of measurement of phenotypes affect GWAS results ?
Values obtained for subcutaneous fat thickness (with thesame definition) from two independent working groupswere correlated with r = 0.72
Differences in GWAS (Genome Wide Association Study) It is recommended that trait values in GWAS experiments
be examined for repeatability before the experiment isperformed. For traits that do not have high repeatability(r < 0.95), two or more independent measurements of thesame trait should be obtained for all samples, andindividuals genotyped that have highly correlated traitmeasurements.
Barendse et al., 2011. BMC Genomics 2011, 12:232
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Outline Some definitions and elements of context
Challenges about beef quality in genetics
Challenges about beef quality in functional genomics
Other perspectives
15
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Large-scale projects in life science are being developed, driven by the desire to explore biology as a whole rather than in pieces
Evolution of research in biology
Predictive biology based on modelling is being developed
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Genes, the author argues, are merely bit players in the game of life. The concept of the gene has been overused.
In future we won't see it as being so important.
We have indeed to look at things differently.
The understanding of biology has reached a turning point.
The central question has shifted from “who are the actors?” [the genes and their products] to “what are the scripts?” [the physiological programmes and interaction between genes].
Fox Keller E (2002)
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Specificity of research on beef quality
A major criteria is tenderness, the measure ofwhich is little repeatable, neither automatisablenor fast and thus with small sets of data.
To solve this problem:- development of tenderness measurement on a large scale and in a standardised way (as in Australia)- search for predictors, the measure of whichcould be fast and automatisable
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Search for beef quality predictors(ex : EU programme ProSafeBeef)
Modelling of beef quality
Prediction of beef quality
Markers of beef quality (SNP, gene or protein
expression levels)
Methods to assess the genomic markers
Experiments in Germany,
Ireland, France
DatabaseMeat Standard
Australia
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Modelling of beef qualityConsumer tests• > 530 000 samples• 40 muscles
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Muscle profiling
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The Whole Data base: BIF-Beef
43 experiments~ 330.153 data621 variables
Age 1 -120 months
Muscle
Longissimus thoracisSemitendinosusTriceps brachii
Rectus abdominis
SexEntire Males
SteersFemales
BreedBlonds d’Aquitaine
CharolaisLimousin
Autres
21000
11000
7600
350
270
4600
550
> 1750
> 1650
1000
> 128000
> 5100
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Comparison of databases: France & USAMuscle Profiling USA BIF-Beef
Animals 142 5197Breeds ?? 20 (Ch, Li, BA, …)Sexe steers ?? mainly young bulls
Variables colour colourExpressible moisture
Ash AshFat Fat, Proteins
Enzymes (LDH, CS, PFK…)Vitamines
Fibres (% and cross area)Emulsion capacity
pH pHCollagen Collagen
Warner-Bratzler Warner-BratzlerFlavour
JuicinessProtocols standard variables
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The “meat quality chip”The GENOTEND programme• 60 mer Oligonucleotides (in situ synthesis)• > 3000 genes (selected genes for muscle growth, fibre types and fat metabolism
from previous studies)• Several probes per gene• 8X15K chip (Agilent technologies)
Pre
dict
edva
lues
R2=0.36 R2=0.28
Observed values
Overall tenderness Shear Force
LT of Charolais Young bulls slaughtered in 2003
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High-throughput protein assay
Guillemin N. et al. 2009. Validation of a dot-blot quantitative technique for large scale analysis of beeftenderness biomarkers. . Journal of Physiology and Pharmacoloy,. 60, 2. 91-97.
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Outline Some definitions and elements of context
Challenges about beef quality in genetics
Challenges about beef quality in functional genomics
Other perspectives
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Where does the Efficiency in Australia come from?
Using the best genetics from the bulls to get best growth rates Using best pasture to feed the cattle Using scientific feeding for profitability in feedlots This means best management and this means: MEASURE,
MEASURE, MEASURE everything Use measurements for bulls, grass, grain Measure the cows, measure the calves, measure the time (it
takes) Only keep the best, kill the rest How do Australian Beef producers make good profits ? They
measure the cost of everything ; They only use the best feed, genetics, management.
Geoff Kirton, 2011, Beijing
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The French strategy still in discussion
A trait NOT easy to measure
High-throughput method to be developed
An unknown but interesting
phenotype
Basic research to develop first: ontology, measurement,
etc
A known and interesting phenotype
A trait easy to measure (ex: weight)
phenotyping infrastructures to be developed
Data acquisition and phenotype recording
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Trends for the future Due to the cost of high-throughput equipments Due to the needs of standardized methods and data sharing
We need a network of coordinated, advanced and standardized phenotyping infrastructures :
• Facilities for measuring well-known traits by classic approaches• Facilities for the development and the measurement of new relevant
traits by imaging techniques, and/or comprehensive description of molecular and metabolic patterns
• To develop strategies for multi-level data integration.
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Conclusions Phenotyping : the rate-limiting step in genomic selection
Phenotyping: the poor partner in integrative biology
Some technological problems to solve before moving to
high-throughput measurements
A challenge: storage and analysis of data
Even more difficult in beef production: tenderness is
difficult to measure as is robustness