ft-ir - jst
TRANSCRIPT
FT-IR�����������
������ ����*� ����**� �� **� ����***
��������������*��������������**��������� !��
***������"#��
Investigation of Degree of Gelatinization of Cooked Rice by FT-IR
Sonoko Ayabe�, Kyoko Tanaka*, Yoko Hamada**,
Midori Kasai** and Keiko Hatae***
Faculty of Health and Welfare, Takasaki University of Health and Welfare,
-1�+, Nakaorui-machi, Takasaki, Gunma, -1*�**--
* Ochanomizu University Senior High School, ,�+�+, Otsuka, Bunkyo-ku, Tokyo ++,�20+*
** Faculty of Human Life and Environmental Sciences, Ochanomizu University,
,�+�+, Otsuka, Bunkyo-ku, Tokyo ++,�20+*
*** School of Home Economics, Wayo Women’s University,
,�-�+, Konodai, Ichikawa, Chiba, ,1,�2/--
����������������
The gelatinization properties of ,* kinds of Indica and Japonica rice were measured by di#erential
scanning calorimetry (DSC), the b-amylase-pullulanase method (BAP) and Fourier-transform infrared spec-
troscopy (FT-IR/ATR). There was a high correlation between the peak temperature for gelatinization and
the apparent amylose content. The FT-IR peak at around +*** cm$+ increased in height with increased
cooking time. The absorbance at this wavenumber reflects an increase in the amount of OH groups, which
are hydrated due to gelatinization of starch. There was a high correlation in the degrees of gelatinization
found by the FT-IR method and the BAP method. The calibration curve obtained in this study indicates
that the measurement of the degree of gelatinization of cooked rice by FT-IR at around +*** cm$+ is an
alternative to the conventional BAP method. It was also found that the hardness and stickiness of cooked
rice as determined by sensory evaluation could be predicted based on the height of the FT-IR absorption
peak. (Received Mar. ,*, ,**0 ; Accepted Jun. +-, ,**0)
%�&'��()*)+,�-./01� 23456789:;-<=>?@A=+BC %��()*)�./D E�@AFG � %H��I�-�/D�> ./JDKD=?L�DMK<=>?NO� �./J'L��P� +Q�R�?S=C ./J�TU�@' boVWX;Y�*ZX[;Y� \BAP�B,BD]�&@A=D�BAP�'^_D�`@a�D��@A � b c�S��DdeOfg5=C 0O-��Ihi�j��P-b=�/k? BAP�-b=./J��/?'�l4mno4S5-B>?m�p0fg� � >fO�qr+�stG
S./JTU�uvDiw0fg5=Cx�� !yTU��]Q@A=z"{|}~��8�7�Z�� \FT-IR�ATRB �-b=./JTU-�<=�pD� ��()*).B�./��/�#()*)/BS�-Q5gS0f� FI-IR-b=�./JTU����D�0fg5=C 4N4� >fO��p'()*)��m�+�$?4g� � �����%�v��+�$?4G�p'�?���OfS5C �>@� %��@'� �����D����S=�)(���?�:� ���¡&���-Q5g� %�v�m¢eG��./£¤+FT-IR�ATR+'5g¥¦� �./JTU�t45uv?4g� FT-IR�o'���+§¨<=>?+&?4GC 0O-� FT-IR�8�7�Z-b � L�S����&©�P�ª�+«=>?����m§¨4GC
***
***�
¬-1*�**-- (®��¯��)° -1�+¬++,�20+* ±�²*�³�´ ,�+�+¬++,�20+* ±�²*�³�´ ,�+�+¬,1,�2/-- µ+®¯¶¯·,¸ ,�-�+-.¹ \Corresponding authorB� [email protected]
Nippon Shokuhin Kagaku Kogaku Kaishi Vol. /-, No. 3, .2+º.22 (,**0) »�*¼ 481\ 31 B
� � � �
+� � �� ��������� +,������� 2��� ��������� ����� ,**.� +*�� ������������� � �!����"#������ �+!�� $%& ����'(�)�*+�,��-"Nursery Sticky rice.� /��-"����0�-1�� ,��/��223���� � ������45#���6�75� ,**-�8��/������ 9:��!��; �<�=�>?@���;MC-3*AA� !%B��2B 3*��C"%*�������� �����45# .D������� ��������� +** g! /� �!�E�"�#�FG� $% ,
H�&'� +*H()I#�*1�� )�+J� /HKB,��-��� !�E�"�#�FG�F#. +./�C"%*/0� ,/* g!�1� ,*D� +LM23I� ?N�OPQ!45��6A7R8�S �SR-*-F, 9T7S8U� ����� ++* V� +,�M� 5�� 1* V� +-�M7��8�I� ?�PV�*1� +/�M"W��#���������2�� ��X'. ,*� .*� 0*� 2*� +**D��Y�Z8�[:!$\"����;"�]� <7=X'^ �HFT-/*�
_>^S� �� 8�S�`?@AaWBCaW + cmDA!C"%*b�cd�X'�EF�� ZX'!G��LH���8�e��I���� JK��������$%&���Z +/ g! ./ ml� 33./�f@g�
h�FG�ij�k�l� �AM-+*� ���;� �ij�k�m �+2 *** rpm, - min� �� nN?oh@��pqr[��� )�+J� -HKB,��I� st<�� ,H!#��Lu��� )5�MvY ,/*�m�NOw"��"%*!Jx��JK������
,� ��� �����������6AJx; �PyJx; DM-0� Qz;{R|}~� ����S� +/*�m�T!Jx�� #��T�uUV �+*/D�� @��Q���hO�hV� �a\�s���?� ���=��.WV0��EF���XSY����11��S +**S�Z&� Y �EF��� EF� -H�DKB,���
-� ����������JK���������'�� �[W� bo
s�N����hNk��V �BAPV�,�!%BEF���(C�+� �� 2* mg! bos�N�� o�hNk����e�\�7�� ��I�j����h��V����]���^ �sh��_`!%"�/����!=("��aW��'��]�� a�O�m bos�N�� �b aNc / IU�mg, k¡t�¢>PQ?� $%& Aero-
� + ������� ����
d£ �� #����
@��Q����
�a\�s���?� ���
XSY�g�
+
,
-
.
/
0
1
2
3
+*
++
+,
+-
+.
+/
+0
+1
+2
+3
,*
ManavoThu Kha
Paw Kywe
Paw Sanbekyar
Nursery Paw San Hmwe Rice
Nurery Nun HtkeTaw Win Rice
Beautiful Myanmar Birinari Rice
Diamond Basmati Rice
Pyas Pon
Tang Pyan
Nursery Shan Rice
Nursery Sticky Rice
Beautiful Myanmar Sticky Rice
e�¤¥¦§25��f��¨��©ª«¬��g®«¬���h¯�Q���°gjV
+-40±*4*c
+.40±*4*ab
+,4/±*4*ef
+.4-±*4.ab
+-4*±*4*de
+-43±*4*b
+,41±*4*ef
+-4*±*4*d
+,41±*4+ef
+.4.±*4+a
+-4/±*4*c
+.4.±*4*a
+,42±*4*e
+.4*±*4,b
+-40±*4*c
+-40±*4*c
+-40±*4*c
+-4/±*4+c
+-4/±*4*c
+-4/±*4+c
04.0±*4*/e
04*2±*4*-g
/4/1±*4*.i
04.1±*4,*e
04+,±*4+-fg
/4/,±*4+/i
14.*±*4,*b
04,3±*4*.f
04.0±*4*1e
042+±*4+2d
24+0±*4*0a
04*+±*4*3gh
/4,.±*4+*j
04,3±*4*.f
/423±*4**h
/41,±*4*1j
/4,2±*4*.j
/4-*±*4*/d
0421±*4*0d
14*3±*4*3c
,04,±*4*a
,-4*±*4-b
,,40±*4*bc
,,4-±*4*bc
,,4-±*4*c
,+4.±*4*cd
,+4,±*4*cd
,*43±*4/d
,*41±+4+d
+14*±*4.e
.4.±*4,g
,4,±*40h
+24*±*4-e
+141±*4.e
+14.±*4,e
+14+±*4-e
+04,±+4,e
+04*±+4*e
/4-±*41f
*4.±*4,i
+24,±*40e
,+4,±*4-b
,*41±*43bc
+34.±*4,d
14.±*4.g
,*42±*4-cd
+/4,±*4+f
+240±*4,e
,+4,±*4.b
,,4+±*40a
+34-±*4.d
+/4.±*4-f
,*4+±*4+cd
,+4/±*4.ab
,*42±*4-c
,+4*±*4,bc
,*4.±*4+cd
+342±*4.d
+24/±*4+e
+243±*4,de
+� hi�mean±SD4
,� a²i : j³k�j³l´M!�Rµb �p¶*4*/� .C�)��'(
��mc·¸¹¸º» n /- ¼ n 3 £ ,**0 � 3 �482 � 32 �
bacter aerogenes���������� ����� ,
IU�mg, ���� ���� ����.� FT-IR�ATR�������D �� ����� ��� �� ���� ��� ��� �� �� ��!�" ,*#� +**#� ��$%�����������&� �'(��)*+����,-������ OH$.����/0����� ��� ,�1�2 .� �34&5!6� 7��890:��;0�<=>�?��"0#$@A�%&B .**C. *** cmD+�'E-,F?()*(� ATR�+0�G��6����HI2J�KL,MN�'-���O JASCO FT-IR .,*� ./-�� 0� PQRS�T�KUV +F(�N SpecacW����X�+ PQRS�TATR01� Y�ZVL�[\]K��� �026� ^_�`a*��b�T$6&��6��
/� �� �� �DSC� ����� +* mg 3c� � �]�d\bVe�0456� 6Qf�78�� ,/�l34&g70:��;09:6&#;6�� hi� ,*#? +<j=1k�>l?m@ O DSC+,*� eQ��ABno� ��&�����p qC!� To�� p DE!� Tc�� p r�*!�Tp� �� p Ls�t� DH� ���6�� KJuv��2� -*�l, F!G�2 ,#�min$6��
0� �������wHIx2� ��JG$y@0z�&� ,*K������;{� 7L|�}��2y�~��M� /$6� 7LN�}��2O���~��M� +$6&� �����Ix�PQGRS0�� /T�IUM�+?,-�� JG0z�&2� ���S=�M� /� Manavo Thu Kha�M� +
$6� y@0z�&2� Manavo Thu Kha�M� /� ���S=�M� +$6���m0�6�V0�W��X>6�+F0z% -�K0z�&Ix@A�� <s�2�Y��ZB[�\]���^� ,*_?}��
1� ����SPSS V+*�`6� ��a�bga$�Wcl��/��Kj�bga�l�����de�l+0�G,-�����Pj�f�,g��/�wHIx����Ph,i�FT-IR��a�jk,i$6&�F�-l�,-��
���� !"#$
+� %&'(m +0>6��;0�����-� 2� Q�����? +,./C+..0�� [��\��? +,.2C+..*�� ��<*n� 2� Q�����? /./C2.,�� [��\��? /.,C1.+�� ����]d���� 2;�{�?2Q�����? +1.*C,0.,�� [��\��? +0.*C+2.*�� ~{�o~{�? *..C/.-�� pq�2Q�����? 1..C,,.+ g,
[��\��? +2./C,+./ g�r�0��G� �-��s"
[% ¡:-�� �¢i��-0��&~Q������t"[��\���G��a�r�"£ � u0����]d���� "£�r�0}�h$"uvh?}-��
,� �� �� �DSC� ������>l?m@ DSC� �����m ,0>6�� ���(w?2� //.3C2*.*#�r�0p 0��(@r�*"x/�i�� p qC!� To� 2� [��\��2(w0Q������G~y � [��\��?2//.3C0,..#1?� Q�����?2 /3.0C1+.0#�r�?}-�� Q�����?2 Nursery Shan Rice /3.0#��Nursery Sticky Rice /3.3#�� Beautiful Myanmar Sticky
Rice 0+.+#� "[��\��� To0z{6&��¤'2[��\���G¥�-�� u0Manavo Thu Kha21+.0#?}G� [��\��?�~ To�y�¦z§1i�G +/#¤S~¥�-�� [��\���p r�*!� Tp� 2 0+./C00.2#� Q�����2 0../C1/./#�r�0��G� Q�����?2� Nursery Shan Rice
0../#� $ Beautiful Myanmar Sticky Rice 00.0#� �Tp"� [��\����$§¨|}��a�>6�¤'2�©&[��\�����G¥!?}G� Manavo Thu
Kha2�¥!�� 1/./#�>6��p Ls�t� DH� ~� Q�����"(wh0¥�ª«0}-�� Nursery Sticky Rice� DH"�~¥�a�>6�"� hi2¬�?}�h$"®6&��$¯4�i�� ~�~{�0��&2;�{���$� DH�l29�i:�-���°�1� 2� ]d���� ����2 To, Tp��
DH$~0¥ �z[%�$�±6&�G� /��?~|��ª«"x/�i�� �:�{ +0K�;�{�0z�& Tp$����]d���� �f�"¥ r²*.3*�:G� ;�{�?2]d���� ����2� Tp"¥�$�4�� 6�6� ~{�0z�&2���S=� Tp
2 0-..#?� ¦z§1i0��? ,LP0y�a?}-�~��� �¢6~�©&�~{�� Tp"y���?2:�-�� ~{�?2]d����³´";�{��Gy � ]d���� $�f�2;�{�$|µ$2�4:�$¯4�i��
-� BAP)*!+,-.������¶��T�0��p �� BAP+?��6�·�, �� +0>6�� ;�{�?2 .*#1?2}1G, ": � �����p 2 0*#�¸4�}�G��¹º0�9� 2*#�� +**#�»&�0��1?0�6 p �"�36�� [��\��2(�h0Q������G~p �"¥�ª«0}-�� ¦z§1i2 +**#0���p �"y/?}-�"� �?2§¨ +**�0�6��k�p ���3³´"¥�-�� ~�[��\��?2(w0�K0��l2d@ � ��T�?2�¢i~§¨ 3/�¤S�p ��>6��
¼"�~ : FT-IR0��p ���+ 483 33 �
������� ���� ������������������������� !"#$%&'( � ��)��� +�*�'+,�-�� .%� /01234�5'6789:; <�+=� >5#$?"����@�ABC����D��)"EF��D�)GHI�'( JK��,�K�+LJ��MN��@O@����"PDQRS*�'(
.� FT-IR�ATR�����T�� BAP��+����) FT-IR�+�3UVWX) YZS[�� FT-IR�+����\] ^_`�aD�bc�'( �de�� �f g� hXi23 j FT-IR�ATR3UVWXS\]�' kl ,m( no:p2V� -.**� , /**� + ,**� + *** cmq+�r .a@s&'( � ,K� + *** cmq+�r p2V�tu2m5vw�x233m y)z��� hXi23 j g�{|�}&�P8:L� hXi23g�)p2V P?��PD~ �Y"s&' kr�*.33m( - .** cmq+� FT-IR ATR�� ��/���� �����O�-� �� O-H
�������O�)�]?��+*m� , /**) + ,** cmq+
� � �����O�p2V@s�(T�� �f g� ^ `�S-�)�� FT-IR�
ATR3UVWXS\]�'��Sl -�*�'( ^ `�@JhXi23)z���g�) + ***
cmq+ �r p2V P?��PD�Y".I�� --�kw�wm ^ `�jS|��+L��?�'-�
� , DSC��������
-� To
k�mTp
k�mTc
k�mDH
kmJ�mgm
+
,
-
.
/
0
1
2
3
+*
++
+,
+-
+.
+/
+0
+1
+2
+3
,*
ManavoThu Kha
Paw Kywe
Paw Sanbekyar
Nursery Paw San Hmwe Rice
Nurery Nun HtkeTaw Win Rice
Beautiful Myanmar Birinari Rice
Diamond Basmati Rice
Pyas Pon
Tang Pyan
Nursery Shan Rice
Nursery Sticky Rice
Beautiful Myanmar Sticky Rice
� ��a����� w1p�v¡¢i£¤�v¥¦i£¤�v0X§2V�2¤¨©�ª
1+4//«*42.a
01412«*412c
0043-«*4/,c
0042,«*4-0c
00412«+4*-c
0241/«*4,-b
0.4+*«*4-/d
0341*«*40.b
014+-«*4/2c
/340*«*4,3g
/343,«+420g
0+4+-«*43+f
/242*«*422gh
//423«+4./j
0+4.2«+4*,ef
/1431«+4/*hi
/3431«+4+.fg
/24-,«*42,h
0,4-/«+4.-e
/14,,«+4.+i
1/4./«*4+.a
1,42-«*4,*c
1+412«*4/,d
1,4*1«*4,.d
1+411«*403d
1,4/-«*4+*c
034*/«*4+2e
1.4+/«*4-,b
1,4-2«*4,2cd
0.4/,«*4-*h
014*,«*4+,f
004/1«*4-+f
0.4+2«*4+0h
0+4.3«*4,3k
0/42,«*4.1g
0.431«*4,2h
0/40*«*4-*g
0.4*-«*4-*i
0042/«+4+.f
0-4.*«*4-,j
2*4**«*4,2a
114,/«*4/,c
1041-«*4/-cd
1043-«*40-c
104+,«*40*d
1043*«*4.-c
1-4/,«*4-1e
1241-«*400b
114-,«*4,3c
03410«*4-*h
1-422«+4.0e
1+4.,«*4/1f
034.*«*4.-h
0041+«+4,1j
1*4/1«*4.3gh
1*4*,«*4,.gh
1*411«*4/,fg
034+2«*40+h
1+432«+4++f
0241,«*4.-i
3400«+401bc
++4*2«*41*ab
3402«+42.bc
341,«+4,3bc
243,«*42.cd
342/«+4+2bc
14/,«+421d
++4*-«*4.0ab
+*4--«*4-.abc
14-0«*402de
++40+«,4+,a
34/+«+4/,c
1430«*4/2d
14-1«*423de
34*.«*40+c
34*+«+4*/c
24,3«+4*2cd
142.«*40/d
14./«,40+d
2432«*43,cd
+m ¬�mean«SD4
,m a®i : zd� zd�¯ ���°! kp±*4*/m ":D�)S*O(
+ �������������,*� .*� 0*� 2*� +**��
��²�³´µ´¶· ¸ /- ¹ ¸ 3 º ,**0 » 3 ¼484 k 34 m
����������� � + ,**�+ *** cm�+���C-C, C-O��������������� �� ��!"����"#�$�%&�+,'+-'� (�� ��)*+,�-.!"�/0�1 + *** cm�+23����4�5.'
6#478�91:5� ;<=��:1>� + ***cm�+23�����?@��5�� ��!"�-:�56#� A � �� �B��C*�>DEF56#4G�9 � � 4�1� �� �C*4HI�JK��� ?@�-.LM����N?�!"*O� A (���:1?@�-.�� �PQRF�ST��UV4W65JK��!"*#���X�Y74ZA95#[\�95�]� ,*^�(�_`abc��d5 FT-IR�ATRef�ghOiI� � j .�k�H�#�1l;m�nop��ef�ghOG� � + *** cm�+23�����?@)*�+,�-�1qr��� � j /� BAPs�
iI�t4uv�9 .^�(� FT-IR�ATRef�gh� + *** cm�+��������"#j +� BAPs��5!"*��"OG� >��w5� BAPs��5!"*#xyF5#� z{|���}� ~���OG� � k6� ,*^�(��:1 FT-IR� + *** cm�+23������/�1 BAPs��5!"*O ��g�� j 0 �A' �G� � !"* �y' #����� �x'�:�%%&�w�� �� ,�s���H]���O�v5#�
y�//-.+x�,*.2r�*.3/. �
#�� ��%&�� *.3/.#�;�����`a��>Z��:)*����d5(ap���:1� FT-IR��������!"*Oi��56#4G�9 �z{|��5#j 0 �A' ��d5!"*#� + *** cm�+
23������� �%4�� >��� j / �A' ��d5 +**��p����a�p���:1� BAPs��> +**��p�#a�t4���5��4w� �FT-IRiI��?�� �p� : ���+ : ,' ��?@p�4iI�� ¡H�P¢£¤O¥¦��5�§�?���w54� 6�?����!"4¨©ª£¤��«¬4 �91®:!£#�5� FT-IR� + *** cm�+23������� C-C, C-O�������������F5���+,' �w5��� p�� C-C, C-O���4 �95+**�¯+�� +**�p�#a�t4°9��� >�#[\�9 � k6�� +**��(���a��:1����±KON�1p�� .²����O?\1iI� �j 0 �B''� ���±KONBF#� �� �C*4³´F5 vLMµ����� 4� ¶^��5�������·4��9!"*�tO�¸¹F56#4�� � 6�#��!"* �y' #����� �x' ��� +]���
� , ���������� � FT-IR�����
� - �������������� FT-IR�ATR����������� !
� . "#$%&�'(� )*+,- � FT-IT�ATR�����
º»�¼ : FT-IR��5!"*iIs 485� 35 '
y�12/./x�,,.- r�*.30+ �
������ �� � +**�������������������� ���!�"#���$%&'( )�*���+#,-.������! FT-IR/0���!�1 0 2A3 456�(7�������89�:;4�1 0 2B3 �5<����&'����=>4?#!�@*A�� BCD�E�FG!HI6�#������JK 1*�LM-3 �4( ����N��0<�����OP#!Q"#� LM( FT-IR�ATRR� BAPR�S@'T#UV�����:;R4W#!�"#�
/� �������XGYZ�( ��['( \6�]�� +*� /74Y^_�R4`A� 2a -3� ['�( T#b�4�cde
fg�h�ijklg�&'mn�*�['�op( ijklg�4Y^qr ,.*( cdefg�4 -.1!�s�t�A� 2pu*.**+3� cdefg�v4mwxyz{|}g~( �����l�j�&����['�o�!YZ6��� mb�����mcdefgh�['�o*A�� �\6�]���( T#b�ijklg�4Y^qr -.2( cdefg�4 ,..!������( ijklg���s�\�!YZ6�� 2pu*.**+3� cdefg�\6YZ_��ijklg�����( �����-.��7A����
0� �� �74 DSC, BAPR( FT-IR�&�XG���
:;���/0`����a .�5<�� XGYZ�&#�\6� FT-IR�&# +**���!��� +
*** cm�+���#����&����!�/0�W'(
� / ��������� BAP�������� FT-IR� + *** cm�+��� !"��2A3 FT-IR���# + ***cm�+������62B3 BAPR�&#���
+ Manavo Thu Kha 1 Dia Basmati Rice 2 Pyas Pon +/ ��
� 0 FT-IR� +*** cm�+������ !"�#$� BAP��������%&2A3 : 2,*( 0*( 2*( +**�( ���3 : ¡¢�+ : ,4£¤( 2B3 : 2+**�( ���3 : ¡¢�+ : .4£¤¥ ,*� ¦ .*� § 0*� ¨ 2*� © +**� ª �
��E«¬®¯° ± /- ² ± 3 ³ ,**0 ´ 3 µ486 2 36 3
DSC����������� � ����������������� FT-IR��� +**��� ���� � + *** cm!+"#�$%&'�(���������) DSC������*���� �+�) �����������'�(��, FT-IR-
./,012345�6�,�7 � ����� 8���
�9 ,:;<=) FT-IR-./,>?<=�@2�<=ABC���DEFGH,I� JK)
Y+L!,*.2-X+!,/./,X,M/.,, rL*.1,/ �
�DEFNOPQ � 66R) Y+���ST� 8����9) X+� +**��U� FT-IR + *** cm!+�$%&�V�
� - ������
WX � ����Y ����Y
+
,
-
.
/
0
1
2
3
+*
++
+,
+-
+.
+/
+0
+1
+2
+3
,*
ManavoThu Kha
Paw Kywe
Paw Sanbekyar
Nursery Paw San Hmwe Rice
Nurery Nun HtkeTaw Win Rice
Beautiful Myanmar Birinari Rice
Diamond Basmati Rice
Pyas Pon
Tang Pyan
Nursery Shan Rice
Nursery Sticky Rice
Beautiful Myanmar Sticky Rice
Z�[\]^_Q`abcd$efghijkeflmijkefnop%&q%rkstuv
.41-w*40*g
.4*0w*413f
-4/3w*4//ef
-4*0w*40+cd
-42-w*4-0ef
-41/w*42+ef
.4*3w*410f
-431w*403f
-4..w*4/.de
-401w*40+ef
-41.w+4*2ef
,4*.w+4+2b
,4+-w*43*b
,422w*41+cd
,4.*w*400bc
,4/-w*411bc
,4-,w*40-b
,4,3w*402b
,4,0w*410b
+4-3w*41*a
+4..w+4*+a
+41/w*42+ab
+400w*4/-a
-4,*w*41*c
+41,w+4*-ab
+42+w*4-0ab
+400w*41*a
+42.w*43,ab
,4+*w*420ab
,4,+w*4/+b
-4+*w+4*-c
.4,,w*4/1de
-42,w*4.1de
-4.1w*40.cd
-4/3w*40/cd
-40/w*41+cd
.4*.w*4--cd
-40-w*412cd
-42.w*4..d
.401w*4/3e
+x =/�meanwSD4
,x ����Y� /yz�{) +yz|P}1� ����Y� /yz��~{) +yz���1�
-x a�g : �������������� �p�*4*/x �16�,�5�
� . ��� �����
FT-IR DSC ��� ����
+**� � To Tp Tc DH +**� � �� ��
+**�a
�b
Toc
Tpd
Tce
DHf
+**�g
�h
��i
��j
+4**
*4/.*
!*4-,
!*4-0
!*4-1
!*4+2
*43+**
*4..*
!*400**
*40/**
+4**
!*4./*
!*4./
!*4.-
*4**
*40,**
*413**
!*40+**
*4/3**
+4**
*433**
*431**
*4//*
!*4/-*
!*4/+*
*41+**
!*412**
+4**
*433**
*40-**
!*4/0*
!*4/+*
*41,**
!*411**
+4**
*403**
!*4/0**
!*4.1*
*41-**
!*410**
+4**
!*4-/
!*4*0
*4-3
!*4--
+4**
*4/3**
!*41/**
*410**
+4**
!*4/,*
*4/3**
+4**
!*43.** +4**
*, ** : ���S.�JK) �Q�Q p�*4*/) p�*4*+R��R��6�,�5�a : +**��U� FT-IR� + *** cm!+���$%&�V�) b : +**��U� FT-IR� + *** cm!+���$%&�V�) c : ������ ��x) d : ��$%&�� ��x) e : ������ ��x) f : ����o�% �J�gx) g : +**��U�����BAPCx) h : ��� �BAPCx) i : ���������Y) j : ���������Y�
���� : FT-IR������-.C 487� 37 x
��� : ���+ : .�� X,�� FT-IR + *** cm+�� �� ��� : ���+ : .� ���� ��� ���� ����� ����� FT-IR��� ������� ��
Y,�,..,/X+!,3.*.X,+.03 r�*.1*1 �
"#�$%�� &&� Y,���'( ����� ���� &%$"� FT-IR��)$���� *�+�,*-�����.�"�� /0%1��23��4�5 /�67��8��69*: � FT-IR� *�� ����'( *�+�����#�.�&�#;<�%�� �=��>?� ��@���#AB'��%C/�+.�D+0�#������/� FT-IR����E���F#G������HI ��1 ��!J�� ��K"L����MN��
� �
,*AOPQRS��TUVWS�#�$X ,Y�HI%&� DSC� BAP�� FT-IR�ATR -� *����� DSCHI�� Z �L)Y[\]�^_'�(�2�;�� :�`���[\]�^_'#�/ab� HI�� Z #�cK8�� FT-IR^d ef + *** cm+)g�� ���� ���#�h*i��Z j8C�cK�� QPkPHI *��+�� OHl,m�-n�C/�� + *** cm+)g�� ��� BAP� *����HI ���/�2�./&#��� FT-IR�0op�1q HI ���.�&�#r)8�� FT-IR *�st�� ��)$������ *�+�� ���#�.�&�#;<�%��
� �
+� 23 u� �=���v4/� 5w=6'(xy� pp.
+-0�+.0 �+331��,� z{|}� ~��7� 89J=!:I 2@��� �; +
<� ��:I �/C� �=�� -,� 0/-�0/3 �+32+��-� >3�?@�A��}�23u}���:I�>?���� �=�� .0� 2.+�2.2 �+33/��
.� Iizuka, K. and Aishima, T., Starch Gelation Process
Observed by FT-IR/ATR Spectrometry with Multi-
variate Date Analysis. J. Food Sci., 0., 0/-�0/2 �l333��/� �BC}� �B�D� �EFG�4H��� HI�:IQPkPI�/st^d ef�J� =��� /*� +0,�+00
�,**-��0� Juliano, B.O., A Simplified assay for milled-rice amylose.
Creal Sci. Today, +0. --.�-.* �+31+�1� K�(}� L�KM� �N �� ��O.P� Q��� : ��J�RS89HI%&�+33*� 3+T�:�`� �/C�� �UJ� .+� ,/�-- �+33.��
2� Nakasato, K., Ono, T., Ishiguro, T., Takamatsu, M.,
Tsukamoto, C. and Mikami, M., Rapid quantitative anal-
ysis of the major components in soymilk using Fouier-
tansfome infrared spectroscopy (FT-IR). J. Food Sci.Tecnol. Res., +*, +-1�+., �,**.��
3� Kelly, J.F.D. and Downey, G., Detection of sugar adulter-
ants in apple juice using fourier transform infrared
spectroscopy and Chemometrics, J. Agric. Food Chem.,/-, -,2+�-,30 �,**/��
+*� Vaski, P.D., Blackwell, J. and Koenig, J.L., Infrared and
raman spectroscopy of carbohydrartes, Carbohydr. Res.+3, ,31�-+* �+31+��
++� �3VW� 89�¡¢X� Q���� £¤Y¥� ZF¦� ~��7[� K\§]� p. +3- �,**-��
+,� Wilson, R.H. and Belton, P.S., A Fourier-transform infra-
red study of wheat starch gels. Carbohydr. Res. +2*, --3�-.. �+322��
+-� Wilson, R.H., Goddfellow, B.I., Belton, P.S., Osborne, B.G.,
Oliver, G. and Russell, P.L., Comparison of Fourier
transform mid infrared spectroscopy and near infrared
reflectatnce spectroscopy with di#erential scanning
calorimetry for the study of the staling of bread. J.Agric. Food Chem., /., .1+�.2- �+33+��
+.� ^_�`a� bc(¨� d�e©� ªf«g� ¬�Nh� i®j� k�¯°lJ *��=���"m�� 89�¡� -,� /+�0* �+32/��
+/� �±n}� ²E©o}�pB�³� ~��7���=����@�����=�%&� �UJ�¡� .1� -.-�-/-
�,***��+0� ©9q´�rs t�uµ¶·�pv«w� ¸¹�,*-g
I�/ *���������=��� .1� 121�13, �,***��
�xy +2T -º ,*z»)� xy +2T 0º +-z»{�
������������������������
z�=!�¡�¡y� ; /- ¤ ; 3 ¼ ,**0 T 3 º488 � 38 �