food & water safety reagents for assay development · to adverse health effects, including...

Meridian Life Science, Inc.

Food & Water Safety Reagents for Assay Development

www.MeridianLifeScience.comInnovative Solutions. Trusted Partner.®Life Science, Inc.®

ISO 9001 Certified & cGMP Compliant

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Meridian Life Science, Inc. (MLS) is a leading large scale manufacturer of antibodies, viral antigens, recombinant proteins, and critical assay reagents. We provide contract R&D, process development, and manufacturing services to the biopharmaceutical and in vitro diagnostic markets along with cGMP biologics manufacturing for Phase I/II clinical trials.

MLS has been providing innovative life science solutions and building trusted partnerships for almost thirty years. Our focus is to offer products and services that help to advance the development of diagnostic assays and vaccine development.

• Commercial scale manufacturing of antigens and antibodies with protein purification expertise

• Full line of immunoassay reagents including blockers, antigens and antibodies

• Large scale production of reagents for molecular assays

• Technical support with assay development expertise

• Dedicated R&D and Manufacturing Teams

• Robust and Mature Quality System

Life Science, Inc.®

ISO 9001 Certified & cGMP Compliant

IMMUNODIAGNOSTICS Antigens & Antibodies Recombinant Proteins

Blocking Agents

CONTRACT SERVICES Antigens & Antibodies

cGMP Phase I/II Viral Vaccines Cell & Viral Banking

MOLECULAR BIOLOGY Nucleotides

Enzymes PCR / qPCR Reagents

CORE EXPERTISECell Culture | Protein Purification | In Vitro & In Vivo MAb Mfg R&D Contract Mfg | Nucleotide Chemistry | PCR / qPCR Enzymes

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Capabilities

www.MeridianLifeScience.com

PARENT COMPANY: MERIDIAN BIOSCIENCE, INC.Diagnostic Test Kits | Founded in 1977; IPO in 1986 | Nasdaq: VIVO Headquartered in Cincinnati, OH | Employees: 500+ | International Presence, 60+ countries

MANUFACTURING SITES

MEMPHIS,TNViral AntigensRecombinant ProteinsIn Vitro Antibody Mfg HAMA Blocking AgentsAntibody Purification Contract R&D and PD Cell and Viral Banking cGMP Vaccine Mfg

LONDON, UKPCR / qPCR Assay DevelopmentRNA AnalysisDNA MW Marker Mfg

BERLIN, DELarge Scale Nucleotides MfgPCR Enzyme MfgProtein Expression and PurificationDNA MW Markers

SYDNEY, AUSCompetent Cells MfgDiagnostic Extraction Controls

BOCA RATON, FLAscites ProductionLarge Scale MAb Mfg55,000 Mice (BALB/c, CAF1)

OVERVIEW OF CAPABILITIES

PRODUCTION SYSTEMSTissue culture flasksSpinner flasksRoller bottles (smooth and ribbed)Cell factoriesWAVE BioreactorsTM

Fermentors

PURIFICATION METHODSUltrafiltrationDiafiltrationTFF (plate or hollow fiber)Centrifugation/ultracentrifugationÄKTA FPLC systems for SEC, Affinity, Ion exchange, HICDialysis, ultracentrifugation, tangential flowExpanded bed chromatographyCentrifugation, gradient centrifugationMicrofluidization, freeze/thaw, chemical lysis

EXPRESSION SYSTEMSMammalian (CHO, Vero, NS0, etc.)Bacterial (E.coli)Yeast ( P. pastoris, S. cerevisiae, etc.)Baculovirus in Sf9Egg-based Virus ProductionVaccinia & Adeno

QUALITY CONTROL AND ANALYTICAL TESTINGMature quality system with dedicated QA/QC and Regulatory teamIn-house testing (e.g. protein concentration, HPLC, ELISA, SDS-PAGE, Western blot)ISO 9001 Quality System Regulations - Applicable sections of 21 CFR 820 Current Good Manufacturing Practices - Applicable Sections of 21 CFR 210, 211

WAVE is a trademark of GE Healthcare

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Capabilities

PARENT COMPANY: MERIDIAN BIOSCIENCE, INC.Diagnostic Test Kits | Founded in 1977; IPO in 1986 | Nasdaq: VIVO Headquartered in Cincinnati, OH | Employees: 500+ | International Presence, 60+ countries

REAGENTS FOR FOOD & WATER SAFETY TESTING

BERLIN, DELarge Scale Nucleotides MfgPCR Enzyme MfgProtein Expression and PurificationDNA MW Markers

In the United States, foodborne disease causes an estimated 48 million illnesses and 3,000 deaths annually, with the economic costs estimated at $152 billion to $1.4 trillion every year. Food safety is a major global, public health concern and requires a highly integrated network of initiatives and practices to assure food safety throughout the entire supply chain from the farm to the consumer. Adding to the complication is the continued increase in international trade and challenges faced governing the import/export regulations of food and agricultural products shipped between countries.

Sources of drinking water are also subject to contamination and require appropriate treatment to remove disease-causing contaminants. There are many sources of water contamination including naturally occurring chemicals and minerals (for example, arsenic, radon, uranium), local land use practices (fertilizers, pesticides, concentrated feeding operations), manufacturing processes, and sewer overflows or wastewater releases. The presence of contaminants in water can lead to adverse health effects, including gastrointestinal illness, reproductive problems, and neurological disorders.

The detection of microorganisms in food and water is an essential part of any quality control or food safety plan. Traditional methods of detecting pathogenic bacteria are often impractical and time-consuming because of the need for growth in culture media followed by isolation, biochemical and/or serological identification, and in some cases, sub-specific characterization. Advances in technology such as antibody and DNA-based assays have made detection and identification faster, more sensitive, more specific, and more convenient than traditional assays. However, even with the application of modern laboratory techniques, there still remains a considerable challenge in identifying the microorganisms responsible for outbreaks. This suggests a large unmet need for better diagnostic tools for foodborne and waterborne diseases.

Meridian Life Science (MLS) is a leading large scale manufacturer of antibodies, viral antigens, recombinant proteins, and critical assay reagents. For almost thirty years, MLS has provided innovative life science solutions for in vitro diagnostic, biopharmaceutical, and biotech manufacturers around the world. The company’s reagents are integral components of commercially available human and animal in vitro diagnostic kits, in-process testing protocols, and high-throughput screening applications worldwide. MLS’ products are designed to help maximize the accuracy, consistency and cost-effectiveness of food and water safety testing.

TOP FIVE FOODBORNE PATHOGENS CAUSING THE MOST ILLNESSES, HOSPITALIzATIONS, AND DEATHS EACH YEAR

PathogenEstimated # of illnesses

90% Credible Interval

%

Norovirus 5,461,731 3,227,078 – 8,309,480 58

Salmonella, nontyphoidal 1,027,561 644,786 –

1,679,667 11

Clostridium perfringens 965,958 192,316 –

2,483,309 10

Campylobacter spp. 845,024 337,031 – 1,611,083 9

Staphylococcus aureus 241,148 72,341 –

529,417 3

Subtotal 91

Thirty-one pathogens are known to cause foodborne illness. Eight of those pathogens account for the vast majority of illnesses, hospitalizations, and deaths. The table above represents the top five pathogens contributing to acquired foodborne illnesses in the United States (Source: CDC).

WAVE is a trademark of GE Healthcare

FOOD & WATER SAFETY CATEGORIESAntibioticsGram Negative BacteriaGram Positive BacteriaVirusesProtozoans (Waterborne) Natural Toxins

For a complete list please visit us at www.MeridianLifeScience.com

OTHER PRODUCT CATEGORIES AVAILABLE

• Infectious Disease • Cancer & Tumor Markers• Cardiac & Cholesterol • Hormones• Blocking Agents • Drugs of Abuse• Clinical Chemistry

Centers for Disease Control and Prevention (2012). Estimates of Foodborne Illness in the United States. Retrieved from http://www.cdc.gov/foodborneburden/López-Campos, G., Martínez -Suárez, J.V., Aguado-Urda M., and López-Alonso, V. (2012). Microarray Detection and Characterization of Bacterial Foodborne Pathogens. Springer: New York. Riemann , H.P. Cliver, D.O. (2005). Foodborne Infections and Intoxications. 3d ed. Academic Press (Elsevier): London, Amsterdam.

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products

CEFTIOFURANTIBODIES

Catalog # SpecificityHost /

SourceClone # Tested Apps

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Format Preservative

G13034M Ceftiofur (includes BSA conjugate) Mouse CH-2034 EIA N Dil.Ascites NaN3

ANTIBIOTICS Antibiotics are a commonly employed group of drugs in farm animals used to stimulate their growth and reduce infection and stock loss. However, the use of antibiotics in food production can pose certain public health risks, as it unintentionally selects for antibiotic resistant bacteria leading to their contamination in animal-derived food. This can create a health hazard if humans become infected with a microorganism strain that cannot be controlled by available treatments. Amidst the threat of increased microbial antibiotic resistance, the FDA issued new guidelines in 2011 urging meat producers to refrain from using antibiotics to promote livestock growth, calling the practice an “urgent public health issue” as a result of their potential to promote the emergence of multi-drug resistant pathogens.

Antibiotic residues can be detected in a range of tissues using various analytical methods such as microbial and enzyme inhibition tests, receptor binding assays, immunoassays, biosensors and HPLC. Sensitive and rapid assays that can be used on the farm, truck and in the plant are needed in order to minimize the impact of potentially transmitting antibiotic-resistant bacteria to humans. Ellis, R.L. American Analytical Detection Systems: Present and Future. Academy of Veterinary Pharmacology & Therapeutics Symposium. Blacksburg, Virginia. 1998

Food and Drug Administration (2012). The Judicious Use of Medically Important Antimicrobial Drugs in Food-Producing Animals (#209). Retrieved from http://www.fda.gov/downloads/animalveterinary/guidancecomplianceenforcement/guidanceforindustry/ucm216936.pdf

Natural News. http://www.naturalnews.com/030132_antibiotics_meat.html#ixzz1znNPBHMZ (July 2012)

PENICILLINANTIBODIES



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Format Preservative

G45216M Penicillin Mouse 112-16229 N/A N Purified NaN3

G45060M Penicillin Mouse Pen9 EIA N Purified NaN3

G13011MPenicillin, Ampicillin, Amoxicillin, Cloxacillin, Hetacillin (includes BSA conjugate)

Mouse CH-2011 EIA N Dil.Ascites NaN3

G01246M Penicillin (Thiazolidine Ring) Mouse B612M EIA N Purified NaN3

ANTIBIOTICS

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productsGRAM NEGATIVE BACTERIA

CAMPYLOBACTER jEjUNIANTIBODIES



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Format Preservative

C01614M Campylobacter jejuni Mouse 57-24 EIA N Dil.Sup. NaN3

C65717M Campylobacter jejuni Mouse BDI717 EIA,WB N Purified NaN3

C65727M Campylobacter jejuni Mouse BDI727 EIA,WB N Purified NaN3

C86082M Campylobacter jejuni Mouse B082M EIA,WB N Purified NaN3



C01482M Campylobacter jejuni Types 1 & 2, non-flagellar Mouse B617M EIA,IFA N Purified None

ANTIGENS


SourceFormat

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Preservative

R14900 Campylobacter jejuni Antigen Culture Purified Y None

GRAM NEGATIVE BACTERIA Gram-negative bacterial pathogens are a worldwide cause of morbidity and mortality. They are responsible for a wide variety of infections including foodborne and waterborne illness, respiratory infections, and sexually transmitted diseases. Environmental sources of the organisms include raw meat or other raw food items, juice and water. The pathogenic capability of Gram-negative bacteria is associated with a unique component of its cell envelope, in particular the lipopolysaccharide layer (also known as LPS or endotoxin) which produces host toxicity. Treating Gram-negative infections can be difficult due to this LPS layer which also serves to protect the bacteria through enabling its resistance to many drugs such as antibiotics, detergents and chemicals. The spread of antibiotic and drug resistance in these bacteria is complicating their treatment and forcing doctors to find alternative therapies or rely on outdated antibiotics that can cause kidney and nerve damage. Traditional detection methods for Gram-negative bacteria that cause foodborne and waterborne outbreaks rely on time-consuming stool cultures and the error-prone Gram stain. Alternative methods which are accurate and rapid are critical to effectively manage the treatment of outbreaks where the infectious dose can be as low as 1–10 cells.

National Institute of Allergy and Infectious Disease (2012). Antimicrobial Drug Resistance. Retrieved from http://www.niaid.nih.gov/topics/antimicrobialresistance/examples/gramnegative/Pages/default.aspx

Todar, K. Bacterial Resistance to Antibiotics. The Microbial World. (2009). July 2012. Retrieved from http://textbookofbacteriology.net/tuberculosis.html

GRAM NEGATIVE ENDOTOXINANTIBODIES



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Format Preservative

C55156M Gram Negative Endotoxin (E. coli 0:111 B4 J5) Mouse 301 EIA N Ascites NaN3


C55159M Gram Negative Endotoxin (E. coli 0:111 B4 J5) Mouse 306 EIA N Ascites None






C01575M Gram Negative Endotoxin (E. coli 0:111 B4 J5) Mouse 305 EIA N Purified None

C55157M Gram Negative Endotoxin (E. coli LPS) Mouse 302 EIA N Ascites None


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MATCHED ANTIBODY PAIRS

LEGIONELLA PNEUMOPHILAANTIBODIES



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Format Preservative

C01590M Legionella pneumophila LPS Mouse B1252M EIA,IFA,Pr N Purified NaN3

C01591M Legionella pneumophila LPS Mouse B1254M EIA,IFA,Pr N Purified NaN3

C86515M Legionella pneumophila LPS Philadelphia 1 strain Mouse 5F4 EIA,Pr N Purified NaN3

C86135M Legionella pneumophila LPS Philadelphia 1 strain Mouse 2F10 DB,Pr N Purified NaN3

C01479M Legionella pneumophila serogroup 1 Mouse B597M EIA,IFA N Purified NaN3

ANTIGENS


SourceFormat

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Preservative

R14610 Legionella pneumophila Serovars 1-7 Culture Whole Cell Y None

E. COLIANTIBODIES



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Format Preservative

C86154M E. coli heat-labile Enterotoxin A-chain Mouse B154M EIA N Purified NaN3

C86157M E. coli heat-labile Enterotoxin A-chain Mouse B157M WB N Purified NaN3

C86232M E. coli heat-labile Enterotoxin A-chain Mouse B123M EIA N Purified NaN3

C86222M E. coli heat-labile Enterotoxin B-chain Mouse B222M EIA,WB N Purified NaN3



C55184M E. coli K99 pili Mouse 401 EIA N Purified NaN3

C55840M E. coli K99 pili Mouse 402 EIA N Purified NaN3

C01533M E. coli O157 Mouse B835M EIA,IFA,Pr N Purified NaN3





C65160M E. coli O157 Mouse BDI160 EIA,IFA N Purified NaN3

C65310M E. coli O157 Mouse 3011 IFA N Purified NaN3

MAV119-499 E. coli O157:H7 Mouse 300-13499 EIA N Purified NaN3


Specificity Capture Antibody Detection Antibody

E. coli O157

C01534MC01535MC01536MC01537M

C01533MC01533MC01533MC01534M


Legionella pneumophila LPSC86135MC01590M

C86515MC01591M

GRAM NEGATIVE BACTERIA

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productsGRAM NEGATIVE BACTERIA


SALMONELLAANTIBODIES



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Format Preservative

C01553M Salmonella Paratyphi A Mouse B926M EIA,IFA N Purified NaN3

C65301M Salmonella species Mouse 6301 EIA N Purified NaN3

C65321M Salmonella species Mouse BID32 EIA N Purified NaN3

C87101M Salmonella species Mouse IFR0111 EIA N Supernatant NaN3

C86109M Salmonella species (A,B & D Groups) Mouse 10D9H DB,EIA,IFA N Purified NaN3

C65635M Salmonella species (A,B,C,D & E Groups) Mouse 5D12A EIA,IFA,WB N Purified NaN3

C65957M Salmonella species (A,B,C,D,E,F & G Groups) Mouse B495M EIA,IFA N Purified NaN3

C65958M Salmonella species (A,B,C,D,E,F & G Groups) Mouse B395M EIA,IFA N Purified NaN3

C01358M Salmonella species (A,B,C,D,E,F & G Groups) Mouse B343M EIA,Pr N Purified NaN3

C01359M Salmonella species (A,B,C,D,E,F & G Groups) Mouse B345M EIA,IFA,Pr N Purified NaN3



C01478M Salmonella typhi Mouse B596M EIA,IFA N Purified NaN3

C01362M Salmonella typhi (LPS) Mouse B348M EIA,IFA N Purified NaN3

C86309M Salmonella typhimurium-LPS Mouse 1E6 EIA N Purified NaN3

ANTIGENS


SourceFormat

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Preservative

R84500 Teichoic Acid, Staphylococcus aureus (Wood Strain) St. aureus Purified N None


Salmonella species

C01358MC01358MC01359MC01359M

C01360MC01361MC01360MC01361M

SHIGELLA BOYDIIANTIBODIES



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Format Preservative

C01480M Shigella boydii Mouse B598M IFA N Purified NaN3

LIPOPOLYSACCHARIDE (LPS) LIPID AANTIBODIES



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Format Preservative

C01613MLipopolysaccharide (LPS gram negative bacteria) (Lipid A)

Mouse 26-5 EIA N Purified NaN3

PSEUDOMONAS MALLEI (BURKHOLDERIA MALLEI)ANTIBODIES



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Format Preservative

C86315M Pseudomonas mallei Mouse 3D11 EIA,WB N Purified NaN3


products


BACILLUS ANTHRACIS (ANTHRAX)ANTIBODIES



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Format Preservative

C86542M Bacillus anthracis Lethal Factor (Anthrax) Mouse B743M EIA,Pr N Purified NaN3

C86543M Bacillus anthracis Lethal Factor (Anthrax) Mouse B414M EIA,Pr N Purified NaN3

C86701M Bacillus anthracis Lethal Factor (Anthrax) Mouse BAL0105 EIA,Pr,WB N Purified NaN3

C86710M Bacillus anthracis Lethal Factor (Anthrax) Mouse BAL0106 EIA,Pr,WB N Purified NaN3

C86110M Bacillus anthracis Protective Antigen (Anthrax) Mouse BAP0101 EIA,WB N Purified NaN3

C86201M Bacillus anthracis Protective Antigen (Anthrax) Mouse BAP0102 EIA,Pr,WB N Purified NaN3


C86410M Bacillus anthracis Protective Antigen (Anthrax) Mouse BAP0104 EIA,WB N Purified NaN3



C86613M Bacillus anthracis Protective Antigen (Anthrax) Mouse C3 EIA,Pr,WB N Purified NaN3

C86702M Bacillus anthracis Spore Antigen (Anthrax) Mouse SA27 EIA,Pr,WB N Purified NaN3

C86910M Bacillus anthracis Spore Antigen (Anthrax) Mouse SA26 EIA,Pr,WB N Purified NaN3

GRAM POSITIVE BACTERIA Gram-positive organisms are the leading pathogens of humans, producing an estimated one-third of all bacterial infections. Treatment of Gram-positive infections with standard antibiotics has recently become problematic as many of these pathogens have developed antimicrobial resistance. In the food industry, Gram-positive bacteria are generally spread through cross contamination between people, however, antimicrobial-resistance in Gram-positive bacteria is reported with increasing frequency in strains isolated from food animals. This increase constitutes a potential risk that resistant strains or resistant genes might spread to humans via the food chain. Foods frequently involved in foodborne intoxications caused by Gram-positive bacteria include meat, poultry, eggs, fish, and dairy products. This rising occurrence of drug resistance in these bacteria combined with their prevalence of infection has created the need for quick and efficient methods for identifying the gram-positive bacteria down to the strain level.

Todar, K. Bacterial Resistance to Antibiotics. The Microbial World. (2009). July 2012. Retrieved from http://textbookofbacteriology.net/tuberculosis.html

Todar, K. Important Groups of Procaryotes. Todar’s Online Textbook of Bacteriology. July 2012. Retrieved from http://textbookofbacteriology.net/tuberculosis.html


Bacillus anthracis Lethal FactorC86710MC86543M

C86701MC86542M

Bacillus anthracis Protective AntigenC86613MC86613MC86501M

C86201MC86301MC86601M

Bacillus anthracis Spore Antigen C86910M C86702M

GRAM POSITIVE BACTERIA

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productsGRAM POSITIVE BACTERIA


CLOSTRIDIUMANTIBODIES



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Format Preservative

C01370M Clostridium botulinum Toxin E (a.a. 2-17) Mouse B369M EIA,IFA,RIA N Supernatant None

C01371M Clostridium botulinum Toxin E (a.a. 2-17) Mouse B367M EIA,IFA,RIA N Supernatant None

C01372M Clostridium botulinum Toxin A (a.a. 1280-1292) Mouse B365M EIA,IFA,RIA N Supernatant None

C01373M Clostridium botulinum Toxin A (a.a. 1280-1292) Mouse B364M EIA,IFA,RIA N Supernatant None

C01374M Clostridium botulinum Toxin B (a.a. 1278-1291) Mouse B362M EIA,IFA,RIA N Supernatant None

C86029MClostridium botulinum Type A Neurotoxin Heavy Chain (a.a. 1177-1195)

Mouse B29M EIA,WB N Purified NaN3

C86033MClostridium botulinum Type A Neurotoxin Heavy Chain (a.a. 869-887)

Mouse B33M EIA,WB N Purified NaN3

C86211M Clostridium botulinum Type A Toxoid Mouse KBA211 EIA,Pr N Purified NaN3

C86468M Clostridium botulinum Type A Toxoid Mouse KBA468 EIA,Pr N Purified NaN3

C86018M Clostridium botulinum Type B Toxoid Mouse KBB18 EIA,Pr N Purified NaN3

C86336M Clostridium botulinum Type B Toxoid Mouse KBB36 EIA,Pr N Purified NaN3

C86042M Clostridium botulinum Type E Toxoid Mouse KBE42 EIA,Pr N Purified NaN3

C86169M Clostridium botulinum Type E Toxoid Mouse KBE169 EIA,Pr N Purified NaN3

C65555M Clostridium difficile Toxin A Mouse BDI555 EIA N Purified NaN3

C65715M Clostridium difficile Toxin A Mouse BDI517 EIA N Purified NaN3

C70472M Clostridium difficile Toxin A Mouse PCG4.1 EIA Y Ascites NaN3

C70517M Clostridium difficile Toxin A Mouse PCG4.1 EIA Y Purified NaN3

C01483M Clostridium difficile Toxin A Mouse B618M IFA N Purified NaN3

C65423M Clostridium difficile Toxin B Mouse B423M EIA N Purified NaN3





C70885M Clostridium difficile Toxin B Mouse 5A8-E11 EIA Y Ascites NaN3

C70888M Clostridium difficile Toxin B Mouse 5A8-E11 EIA Y Purified None

C01393M Clostridium tetani, Tetanus toxin (tetanospasmin, TeNT) Mouse B416M EIA,WB,Neut N Purified NaN3



ANTIGENS


SourceFormat

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Preservative

R01372 Clostridium botulinum Toxin A (a.a. 1280-1292) Synthetic Purified N None

R01374 Clostridium botulinum Toxin B (a.a. 1278-1291) Synthetic Purified N None

R01370 Clostridium botulinum Toxin E (a.a. 2-17) Synthetic Purified N None


Clostridium botulinum Type A Toxoid C86211M C86468MClostridium botulinum Type B Toxoid C86336M C86018M

Clostridium botulinum Type E Toxoid C86169M C86042M


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LISTERIAANTIBODIES



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Format Preservative

C86020M Listeria monocytogenes Mouse LZA2 EIA,WB N Purified NaN3

C86030M Listeria monocytogenes Mouse LZH1 EIA,Pr,WB N Purified NaN3

C86503M Listeria monocytogenes Mouse LZG5 EIA,WB N Purified NaN3

C86713M Listeria monocytogenes Mouse LZG7 EIA,WB N Purified NaN3

C86920M Listeria monocytogenes Mouse LZF7 EIA,Pr,WB N Purified NaN3


Listeria monocytogenes C86030M C86920M

STREPTOCOCCUS ANTIBODIES



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Format Preservative

C55560M Streptococcus agalactiae Mouse BDI560 EIA N Purified None

C01532M Streptococcus Group A Mouse 501 EIA N Purified NaN3

C55504M Streptococcus Group A Mouse 504 EIA N Ascites NaN3

C55769M Streptococcus Group A Mouse 501 EIA N Ascites NaN3

C65421M Streptococcus Group A Mouse 042 EIA N Purified NaN3

C65043M Streptococcus Group A (carbohydrate) Mouse 3401 EIA,LF,Pr N Purified NaN3

C65721M Streptococcus Group B Mouse 072 EIA,IFA,IP N Purified NaN3

C55230M Streptococcus pneumoniae, Surface Protein A Mouse BDI202 EIA N Purified None

C55220M Streptococcus pneumoniae, Surface Protein A Mouse BDI020 EIA N Purified None


Streptococcus Group A C65043M B65150G

GRAM POSITIVE BACTERIA

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productsGRAM POSITIVE BACTERIA


STAPHYLOCOCCUSANTIBODIES



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Format Preservative

C01543M Staphylococcal Enterotoxin B (SEB) Mouse B874M EIA,Pr N Purified NaN3

C01544M Staphylococcal Enterotoxin B (SEB) Mouse B875M EIA,Pr N Purified NaN3

C55570M Staphylococcus aureus Mouse 702 EIA N Purified NaN3



C86203M Staphylococcus aureus Enterotoxin Mouse S5 EIA,WB N Purified NaN3



C86104M Staphylococcus aureus Enterotoxin A (SEA) Mouse B007M EIA,WB,Pr N Purified NaN3







C14140M Staphylococcus aureus Enterotoxin B Mouse SEB EIA N Purified None

C86220M Staphylococcus aureus Enterotoxin B Mouse S222 EIA,Pr,WB N Purified NaN3

C86400M Staphylococcus aureus Enterotoxin B Mouse S643 EIA,Pr,WB N Purified NaN3

C01273M Staphylococcus aureus Enterotoxin G (SEG) Mouse B189M EIA,WB,Pr N Purified NaN3

C01274M Staphylococcus aureus Enterotoxin G (SEG) Mouse B191M EIA,WB,Pr N Purified NaN3

C01271M Staphylococcus aureus Enterotoxin I (SEI) Mouse B186M EIA,WB,Pr N Purified NaN3

C01272M Staphylococcus aureus Enterotoxin I (SEI) Mouse B187M EIA,WB,Pr N Purified NaN3


Staphylococcus aureus Enterotoxin A (SEA)

C86104MC86104MC86107MC86205MC86208MC86411MC86510MC86712M

C86205MC86208MC86104MC86104MC86104MC86104MC86104MC86205M

Staphylococcus aureus Enterotoxin B (SEB)

C86220MC01543MC01544M

C86400MC01544MC01543M

Staphylococcus aureus Enterotoxin G (SEG) C01273M C01274M

Staphylococcus aureus Enterotoxin I (SEI) C01271M C01272M


products

HEPATITIS AANTIBODIES



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Format Preservative

C65868M Hepatitis A Virus (HAV) Mouse BDI868 EIA,IHC N Purified NaN3

C65881M Hepatitis A Virus (HAV) Mouse 818 EIA,IFA,IHC N Purified NaN3

C65885M Hepatitis A Virus (HAV) Mouse 581 EIA N Purified NaN3

C86318M Hepatitis A Virus (HAV) Mouse MK-01 EIA,WB N Purified NaN3

ANTIGENS


SourceFormat

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PreservativeTested Apps

8198 Hepatitis A Virus (HAV) Antigen, >60% Viral ProteinFRhK-4

CellsPartially Purified Y NaN3 EIA,WB

8505Hepatitis A Virus (HAV) Antigen, Concentrate, >60% Viral Protein

FRhK-4 Cells

Partially Purified Y NaN3 EIA,WB

R18110 Hepatitis A Virus (HAV) P3C, Recombinant E. coli Purified N None EIA,WB

R18910 Hepatitis A Virus (HAV) P2C, Recombinant E. coli Purified N None EIA,WB

R18210 Hepatitis A Virus (HAV) P2C-P3A, Recombinant E. coli Purified N None EIA,WB

R18710 Hepatitis A Virus (HAV) VP1, Recombinant E. coli Purified N None EIA,WB

R18810 Hepatitis A Virus (HAV) VP1-P2A, Recombinant E. coli Purified N None EIA,WB

R18610 Hepatitis A Virus (HAV) VP3, Recombinant E. coli Purified N None EIA,WB

R18510 Hepatitis A Virus (HAV) VP4-VP2, Recombinant E. coli Purified N None EIA,WB

VIRUSES Half of the reported foodborne disease outbreaks in developed countries have no identified agent and literature to date suggests that these outbreaks are caused by viruses. The Center for Disease Control and Prevention (CDC) estimated that 67% of foodborne illnesses in 1999 in the U.S. alone, were caused by a Norwalk-group of viruses (noroviruses) (Mead et al, 1999). Viruses require a host in order to multiply and human infection can occur following consumption of contaminated food, person-to-person body contact, or release of aerosols; viruses cannot grow in food. Contamination of food may occur either during preparation and serving by infected food handlers or by contact with sewage or sewage-polluted water.

Viruses represent one of the most unrecognized causes of foodborne and waterborne outbreaks. Furthermore, highly pathogenic diseases which have been traditionally limited to animals are crossing the species boundary to humans, such as avian and swine influenza. This suggests the need for adequate, rapid diagnostic tools to detect viruses in food which overcome the problematic requirement for a living host or animal tissue for growth, combined with the low level of contamination in foods. Traditional methods have relied on cell culture and complex extraction methods but these techniques are not suitable for routine application and recovery rates remain poor, prompting the need for updated virus-detection procedures which are cost-effective and allow for practical implementation.

News Medical. http://www.news-medical.net/news/2008/04/09/37149.aspx. (July 2012)

Mead et al. (1999). Food-related illness and death in the United States. Emerging Infectious Diseases 5(5):607-625. http://www.cdc.gov/ncidod/eid/vol5no5/pdf/mead.pdfRiemann , H.P. Cliver, D.O. (2005). Foodborne Infections and Intoxications. 3d ed. Academic Press (Elsevier): London, Amsterdam.

World Health Organization (2008). Viruses in Food: Scientific Advice to Support Risk Management Activities. Retrieved from http://www.who.int/foodsafety/publications/micro/Viruses_in_food_MRA.pdf

VIRUSES

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productsVIRUSES



ROTAVIRUSANTIBODIES



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Format Preservative

C65194M Rotavirus Mouse B194M EIA,IFA,Pr N Purified NaN3



MCV41-121 Rotavirus Mouse 213-10121 EIA,IFA Y Purified NaN3

MCV41-124 Rotavirus Mouse 213-15124 EIA,IFA Y Purified None

C65521M Rotavirus broad reactivity Mouse 521 EIA,WB N Purified NaN3

C66130M Rotavirus Group A specific Antigen VP6 Mouse 3F7 WB N Purified NaN3

C01295M Rotavirus Group A Specific Antigen, VP6 Mouse B316M N/A N Purified NaN3

C86310M Rotavirus Group Specific Antigen Mouse 3C10 EIA,WB,LF N Purified NaN3

C11222M Rotavirus NCDV (Nebraska Calf Diarrhea Virus), p43 (vp6) Mouse B260M N/A N Purified NaN3

ANTIGENS


SourceFormat

Dry Ice

PreservativeTested Apps

7844 Rotavirus Antigen (Strain SA-11) >60% Viral ProteinMA104 Cells

Partially Purified Y None EIA


RotavirusC65196MC65196M

C65194MC65197M

PRION PROTEINANTIBODIES



Dry Ice

Format Preservative

Q06120M Prion Protein (a.a. 93-109) Mouse 6D11 EIA,IHC,WB Y Purified None

Q06850M Prion Protein (PrP) Mouse 7D9 EIA,IHC,WB Y Purified None

Q65115M Prion Protein (PrP) Mouse BDI115 EIA,IHC,WB N Purified NaN3

NOROVIRUSANTIBODIES



Dry Ice

Format Preservative

C01257M Norovirus, capsid protein Mouse B113M EIA,Pr N Purified NaN3

C01258M Norovirus, capsid protein Mouse B114M EIA,Pr N Purified NaN3


NorovirusC01257MC01258M

C01258MC01257M


products


PROTOzOAN (WATERBORNE)

CRYPTOSPORIDIUM PARVUM ANTIBODIES



Dry Ice

Format Preservative

C65760M Cryptosporidium parvum (Oocysts) Human/Bovine Mouse 107 EIA N Purified NaN3

C01484M Cryptosporidium parvum, membrane antigen Mouse B621M EIA,IFA N Purified NaN3

PROTOzOAN (WATERBORNE) Over recent decades, parasitic protozoa have been recognized as having great potential to cause waterborne and foodborne disease. In the developing world, contamination of drinking water with protozoan pathogens poses a serious threat to millions of people, however, periodic outbreaks are also seen in the developing world, often caused by the protozoan parasites Cryptosporidium sp., Giardia sp., and Entamoeba histolytica. Parasite-free drinking water relies on conventional water treatment — a regimen of coagulation/flocculation, sedimentation, filtration and disinfection which normally removes protozoan parasites effectively. Simple, cheap methods for the identification of protozoans in environmental samples are still needed as the standard procedures for the detection of many species were developed several decades ago and rely on microscopy identification of cysts and trophozoites.

Bouzid, M. Steverding, D. and Tyler, K.M. (2008). Detection and surveillance of waterborne protozoan parasites Current Opinion Biotechnololg,19(3):302-6.

Dawson, D. (2005). Foodborne protozoan parasites. International Journal of Food Microbiology, 103: 207– 227.

ENTAMOEBA HISTOLYTICAANTIBODIES



Dry Ice

Format Preservative

C65535M Entamoeba histolytica Mouse BDI535 EIA N Purified NaN3

C65553M Entamoeba histolytica Mouse BDI553 EIA,IHC(p) N Purified NaN3

C55170M Entamoeba histolytica HK-9 Mouse 5001 EIA N Ascites NaN3

C55500M Entamoeba histolytica HK-9 Mouse 5000 EIA N Ascites NaN3

GIARDIAANTIBODIES



Dry Ice

Format Preservative

C01597M Giardia lamblia Mouse B1261M EIA,WB,IFA,Pr N Purified NaN3

C01598M Giardia lamblia Mouse B1262M EIA,IFA,Pr N Purified NaN3

C65361M Giardia lamblia Mouse BDI361 EIA,IFA,WB,Pr N Purified NaN3

C65612M Giardia lamblia Mouse BDI612 EIA,IFA,WB,Pr N Purified NaN3

C65672M Giardia lamblia Mouse BDI276 EIA,IFA,Pr N Purified NaN3

C01485M Giardia lamblia, extracellular domain Mouse B623M IFA N Purified NaN3


Giardia lamblia

C65672MC65672MC01598MC65672MC65612M

C65361MC01597MC65361MC65361MC65361M

- 15 -

productsNATURAL TOXINS

AFLATOXINANTIBODIES



Dry Ice

Format Preservative

K55980M Aflatoxin Mouse AFA-1 EIA N Purified None

NATURAL TOXINS Foods can contain natural toxins as a result of product spoilage, damage and mold growth. Common plant-based toxins such as lectins (found in many types of beans), glycolalkaloids (found in potatoes), and trypsin inhibitors (found in soybeans) are not toxic in humans unless consumed in high amounts. Contamination of fish with toxins can occur from naturally occurring marine algae (phycotoxins) and many fungi and mold can excrete natural secondary toxic compounds called mycotoxins which exert harmful effects in humans and animals at very low levels. Bacteria can also produce toxins such as Vibrio cholerae, the bacterium responsible for cholera which is transmitted by the ingestion of raw or undercooked seafood and drinking contaminated water.

Conventional analytical methods for the detection of toxins typically include chromatographic techniques which can be slow and expensive since they require highly-trained personnel and costly equipment. The need for simple, rapid, and cost-effective methods for detecting natural toxins that can be handled on-site, have become increasingly important to food manufacturers in order to keep commodities and products moving rapidly through the marketing channels, saving time and money. The advance of immunological techniques is becoming more useful for toxin detection due to their improved specificity and sensitivity.

Centers for Disease Control and Prevention (2012). Laboratory Methods for the Diagnosis of Vibrio cholera. Retrieved from http://www.cdc.gov/cholera/laboratory.html

Miliotis, M.D., Bier, J.W. (2003). International Handbook of Foodborne Pathogens. New York: CRC Press.

ASPERGILLUSANTIBODIES



Dry Ice

Format Preservative

C65514M Aspergillus Mouse BDI514 EIA N Purified NaN3

ANTIGENS


SourceFormat

Dry Ice

Preservative

R14200 Aspergillus fumigatus Culture Partially Purified Y None


products

VEROTOXINANTIBODIES



Dry Ice

Format Preservative

C65583M Verotoxin I (Shiga-like) B subunit (SLT-1b, STX-1b) Mouse 357 EIA,Neut N Purified NaN3

C65585M Verotoxin II (Shiga-like) A subunit (SLT-2a, STX-2a) Mouse 557 EIA,Neut N Purified NaN3

C65582M Verotoxin II (Shiga-like) B subunit (SLT-2b, STX-2b) Mouse 257 EIA,Neut N Purified NaN3

NATURAL TOXINS

MICROCYSTINANTIBODIES



Dry Ice

Format Preservative

C01515M Microcystin-LR Mouse B762M EIA N Purified NaN3

C01516M Microcystin-LR Mouse B764M EIA N Purified NaN3

HT-2 TOXINANTIBODIES



Dry Ice

Format Preservative

C01389M HT-2 toxin Mouse 2108 N/A N Purified None

C01390M HT-2 toxin Mouse 2109 N/A N Purified None

C01391M HT-2 toxin and T-2 toxin Mouse B413M N/A N Purified NaN3

C01392M HT-2 toxin and T-2 toxin Mouse B425M N/A N Purified NaN3

ORDERING INFORMATION

TO ORDER

Customers may order on-line at www.MeridianLifeScience.com or by fax, mail or email the purchase order. Please use the full product description, product catalog number, unit size, quantity required, and include any special instructions (i.e., lot number).

ADDRESS TELEPHONE FAX5171 Wilfong Road 901.382.8716 901.382.0027Memphis, TN 38134 800.327.6299

EMAILFor Orders: [email protected] Inquiries: [email protected]

PAYMENT

Terms are Net 30 days and all payments must be made in U.S. dollars. Payment is accepted by the following methods, (please inquire for specific details):Check Wire transferVISA, MasterCard and American Express

PRODUCT SPECIFICATIONS

Specification Sheets are available on all products. Please call the Client Services Department or visit our website: www.MeridianLifeScience.com. Products are “For Research Use Only (RUO). Not for Use in Diagnostic Procedures.”

WHY PARTNER WITH MLS?

• Unparalleled experience and proven track record (almost 30 yrs)

• Core expertise in cell culture, ascites, protein production and purification

• Flexibility in scale and production systems

• Complete R&D and Process Development services

• Robust quality system

• All manufacturing conducted under ISO 9001 and cGMP

• Continuous improvement through Lean Six-Sigma

• Complete confidentiality

Innovative Solutions. Trusted Partner.®Life Science, Inc.®

Meridian Life Science, Inc.5171 Wilfong Road | Memphis, TN 38134901.382.8716 • 800.327.6299

August 2012

food & water safety reagents for assay development · to adverse health effects, including...

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