Field Evaluation of Host Plant Resistance in Strawberry Against Verticillium Wilt, Macrophomina

Crown Rot and FusariumWilt

G.J. Holmes, S. M. Mansouripour, J. Winslow, B. Gomez‐Soto, B. Liu and T. Gordon

New Strawberry Center Staff

Peter Shearer, Ph.D.Entomologist

Drew SummerfieldFarm Manager

Shashika HewavitharanaPlant Pathologist

Pathology Automation

Entomology

NEXT‐GEN DISEASE RESISTANCE BREEDING

1 = highest priority; 2 = medium priority; 3 = lowest priority; N = non‐priority.  SD = short‐day, DN = day‐neutral, and NP = nursery production.

R‐gene = resistance gene, QTL = large‐effect quantitative trait locus, complex = polygenic,  complex genetics, and ? = unknown or hypothesized.

Portola Monterey

VERTICILLIUM TRIAL 2015

San Andreas Albion

Fusarium wilt

Natural

Cal PolyField 25

Verticillium wilt

Natural

Cal PolyField 35b

Macrophominacrown rot

Artificial

Monterey Bay AcademyLocation

Disease

Inoculum

90 genotypes30 cultivars60 elite lines4 replicates

90 genotypes30 cultivars60 elite lines4 replicates

14 DAP

Reading plant mortality every two weeks

Drone imaging every two weeks after planting

0.00

10.00

20.00

30.00

40.00

50.00

60.00

70.00

80.00

90.00

100.00

UC‐ 7

UC‐ 6

UC‐ 8

UC‐ 12

UC‐ 22

Camino Re

alMarqu

isPL 05‐10

0RUC‐ 15

Petaluma

UC‐ 16

UC‐ 9

UC‐ 3

San An

drea

sUC‐ 4

Albion

LC‐ 6

UC‐ 21

Swee

t Ann

DR‐ 1

Celin

eFron

teras

Cabrillo

PSI‐ 4

UC‐ 11

UC‐ 14

DR‐ 4

PSI‐ 2

UC‐ 13

UC‐ 18

Scarlet

PL 09‐49

PS 927

1Man

resa

UC‐ 5

Mon

terey

PSI‐ 3

UC‐ 26

Pasade

naLC‐ 4

Lucia

UC‐ 25

Encinita

UC‐ 23

Alafia

BG 3.324

LC‐ 5

PSI‐ 6

PSI‐ 7

Sabrina

Anya

El Dorad

oUC‐ 2

Big Su

rUC‐ 1

UC‐ 19

Amad

oUC‐ 10

PL 02‐32

UC‐ 17

PL 09‐11

Ruby

 June

PE 7.205

9BG

 6.302

4BG

 959

UC‐ 24

PSI‐ 1

UC‐ 20

DR‐ 5

Del Rey

Pasillo

PSI‐ 5

BG 4.352

LC‐ 3

Osceo

laMystic

LC‐ 1

DR‐ 6

DR‐ 7

LC‐ 2

Laredo

Ode

ssa

Meg

anPilgrim

BG 197

5Festival

BG 4.367

DR‐ 3

Benicia

Mortality (%

)

Cultivar / Coded genotype

Verticillium wilt 2017

Marquis

Amado

Benicia

UC PSI Plan

asa

LC Driscoll’s

Verticillium wilt 2017

0.00

10.00

20.00

30.00

40.00

50.00

60.00

70.00

80.00

90.00

100.00

UC‐5

DR‐8

DR‐20

Man

resa

UC‐6

DR‐7

UC‐4

UC‐9

UC‐14

Petaluma

Marqu

isUC‐11

PS 927

1DR

‐13

DR‐11

Amad

oDR

‐3PE

 3.211

PSI‐5

DR‐17

DR‐14

Fron

teras

PE 3.221

PS 3.108

Sensation

Del R

eyUC‐15

PE 6.203

6PE

 7.205

9PS

I‐6DR

‐6PL‐02‐32

Big Su

rDR

‐10

Cabrillo

PSI‐7

DR‐16

UC‐1

PL‐05‐10

0RBG

 431

6En

chan

teUC‐13

Celin

ePS

I‐8LC‐5

UC‐12

Beau

tyPS

I‐4UC‐10

UC‐16

BG 3.324

DR‐9

Osceo

laBG

 4.367

PL‐09‐11

UC‐7

UC‐3

LC‐2

UC‐8

PS 501

6BG

4.35

2LC‐8

PL‐08‐22

RUC‐2

LC‐7

DR‐1

Maverick

DR‐19

PSI‐2

DR‐12

Elyana

DR‐5

PSI‐1

DR‐4

DR‐15

Laredo

Swee

t Ann

Winterstar

FL 13.26

‐134

Festival

DR‐18

Radian

ceBG

 6.302

4DR

‐2LC‐9

LC‐3

PSI‐3

LC‐4

Ruby

 June

Mon

terey

LC‐6

Mortality (%

)

Cultivar/Coded genotype

Verticillium wilt 2018

Marquis

Osceola

Festival

Verticillium Field

Week43

Week30

VERTICILLIUM TRIALJuly 15, 2017

Monterey (Vert‐S)

0

10

20

30

40

50

60

70

80

90

100

30‐Oct 30‐Nov 31‐Dec 31‐Jan 28‐Feb 31‐Mar 30‐Apr 31‐May 30‐Jun 31‐Jul

Green

 cov

erage(%)

Date

Mortality (%

)

`

Manresa (Vert‐R)

0

10

20

30

40

50

60

70

80

90

100

0

10

20

30

40

50

60

70

80

90

100

30‐Oct 30‐Nov 31‐Dec 31‐Jan 28‐Feb 31‐Mar 30‐Apr 31‐May 30‐Jun 31‐Jul

Green

 cov

erage(%)

Green

 cov

erage(%)

Date

=Manresa‐R=Monterey‐S

`

0

10

20

30

40

50

60

70

80

90

100

Petaluma Sweet Ann Celine Fronteras Cabrillo Manresa Monterey Big Sur Amado Ruby June Del Rey Osceola Laredo Festival

Plan

t Mortality (%

)

Verticillium wilt

2017 AVG = 30.12018 AVG = 11.6

∆ = 61.5%

March 1, 2017

MACROPHOMINA TRIAL

14 DAP

August 1, 2017MACROPHOMINA TRIAL

Festival

Fronteras

Manresa

0.0

10.0

20.0

30.0

40.0

50.0

60.0

70.0

80.0

90.0

100.0

Osceo

laMan

resa

UC‐ V

UC‐ G

PSI‐ D

UC‐ R

UC‐ B

UC‐ F

UC‐ U

DR‐ A

Meg

anGrena

daPS

I‐ B

UC‐ Z

PL 02‐32

UC‐ i

UC‐ M

Laredo

PS 927

1UC‐ O

UC‐ N

BG 6.302

4PS

I‐ C

PSI‐ G

UC‐ W

Alafia

DR‐ F

UC‐ C

DR‐ D

Amad

oUC‐ S

UC‐ E

Petaluma

Encinita

Marqu

isSan An

drea

sUC‐ T

Anya

BG 4.352

UC‐ Q

Big Su

rUC‐ H

UC‐ A

BG 4.367

BG 197

5PL 09‐11

Sabrina

UC‐ P

BG 3.324

Del Rey

BG 959

LC‐ F

UC‐ K

Mystic

DR‐ G

Portola

PL 05‐10

0RFron

teras

PSI‐ F

UC‐ L

DR‐ C

LC‐ C

PSI‐ A

Pasade

naPS

I‐ E

Albion

PE 7.205

9DR‐ E

LC‐ E

UC‐ D

Albion

Celin

eLC‐ B

PL 09‐49

Lucia

LC‐ D

Pilgrim

Radian

cePa

sillo

Ode

ssa

El Dorad

oSw

eet A

nnMon

terey

UC‐ X

Scarlet

LC‐ A

UC‐ Y

Festival

Ruby

 June

UC‐ J

Mortality (%

)

Cultivar/Coded genotype

Macrophomina 2017

Manresa

Del Rey

Ruby June

UCPSI Pl

anasa

LC Driscoll’s

Macrophomina 2017

0.0

10.0

20.0

30.0

40.0

50.0

60.0

70.0

80.0

90.0

100.0

DR‐R

Laredo

UC‐K

PS 927

1Osceo

laUC‐M

DR‐E

DR‐H

DR‐K

DR‐N

Man

resa

DR‐L

UC‐L

DR‐T

DR‐D

DR‐S

DR‐J

Amad

oBG

 6.302

4UC‐i

DR‐G

BG 431

6Grena

daPE

 6.203

6PS

I‐CPS

I‐DDR

‐OBG

 4.367

Big Su

rDR

‐iEn

chan

teMarqu

isSensation

UC‐E

UC‐O

Celin

eDR

‐MFron

teras

LC‐B

Maverick

PE 7.205

9UC‐J

PL‐09‐11

PSI‐H

BG 4.352

BG 3.324

Del Rey

DR‐F

DR‐Q

PSI‐E

PSI‐F

UC‐A

UC‐N

PE 3.211

PSI‐B

Petaluma

PSI‐G

PS 501

6PL‐02‐32

PS 3.108

DR‐C

UC‐D

DR‐P

LC‐i

UC‐F

Mon

terey

LC‐G

PE 3.221

Beau

tyFL 13.26

‐134

Albion

Radian

cePS

I‐ALC‐H

UC‐B

DR‐A

UC‐C

PL‐08‐22

RLC‐E

Cabrillo

Festival

LC‐C

UC‐G

UC‐H

PL‐05‐10

0RSw

eet A

nnDR

‐BRu

by Ju

neLC‐F

LC‐D

Mortality (%

)

Cultivar/coded genotype

Macrophomina 2018

Manresa

Del Rey

Ruby June

Verticillium Field Macrophomina Field

Week43

Week30

Ruby June (Mac‐S)

0

10

20

30

40

50

60

70

80

90

100

30‐Oct 30‐Nov 31‐Dec 31‐Jan 28‐Feb 31‐Mar 30‐Apr 31‐May 30‐Jun 31‐Jul

Green

 Cov

erage (%

)

Date

Mortality (%

)

`

Osceola (Mac‐R)

0

10

20

30

40

50

60

70

80

90

100

0

10

20

30

40

50

60

70

80

90

100

30‐Oct 30‐Nov 31‐Dec 31‐Jan 28‐Feb 31‐Mar 30‐Apr 31‐May 30‐Jun 31‐Jul

Green

 Cov

erage (%

)

Green

 Cov

erage (%

)

Date

=Ruby June‐S=Osceola‐R

`

0

10

20

30

40

50

60

70

80

90

100

Laredo Amado Petaluma Marquis Big Sur Del Rey Fronteras Albion Celine Sweet Ann Monterey Festival Ruby June

Mortality (%

)

Macrophomina 2017 & 2018

2018 AVG= 24.02017 AVG= 46.8

∆ = 48.7%

50

60

70

80

90

100

110

1‐Oct

8‐Oct

15‐O

ct22

‐Oct

29‐O

ct5‐Nov

12‐Nov

19‐Nov

26‐Nov

3‐Dec

10‐Dec

17‐Dec

24‐Dec

31‐Dec

7‐Jan

14‐Jan

21‐Jan

28‐Jan

4‐Feb

11‐Feb

18‐Feb

25‐Feb

4‐Mar

11‐M

ar18

‐Mar

25‐M

ar1‐Ap

r8‐Ap

r15

‐Apr

22‐Apr

29‐Apr

6‐May

13‐M

ay20

‐May

27‐M

ay3‐Jun

10‐Jun

17‐Jun

24‐Jun

1‐Jul

8‐Jul

15‐Jul

22‐Jul

29‐Jul

5‐Au

g

Tempe

rature (F

)

Date

Daily Max TemperaturePlantingTermination

Heat Wave

Water stress

50

60

70

80

90

100

110

1‐Oct

8‐Oct

15‐O

ct22

‐Oct

29‐O

ct5‐Nov

12‐Nov

19‐Nov

26‐Nov

3‐Dec

10‐Dec

17‐Dec

24‐Dec

31‐Dec

7‐Jan

14‐Jan

21‐Jan

28‐Jan

4‐Feb

11‐Feb

18‐Feb

25‐Feb

4‐Mar

11‐M

ar18

‐Mar

25‐M

ar1‐Ap

r8‐Ap

r15

‐Apr

22‐Apr

29‐Apr

6‐May

13‐M

ay20

‐May

27‐M

ay3‐Jun

10‐Jun

17‐Jun

24‐Jun

1‐Jul

8‐Jul

15‐Jul

22‐Jul

29‐Jul

5‐Au

g

Tempe

rature (F

)

Date

20172018

TerminationPlanting

Water stress

Daily Maximum Temperature

Fusarium oxysporum f.sp. fragariae

Monterey Bay Academy

FUSARIUM TRIAL 2015

FUSARIUM TRIAL 2015

9.9

16.4

26.7

33.3

32.3

55.2

53.2

75.7

96.5

0.00 20.00 40.00 60.00 80.00 100.00

SweetAnn 

Monterey  

Radiance  

Albion  

Sabrina  

Grenada  

Sensation  

Winterstar  

Ventana

San Andreas 4.1

Festival 2.3

Portola 1.7

Safari 0.9

Fronteras 0.6

Petaluma 0.3

Percent Mortality on July 23, 2015

FUSARIUM TRIAL 2015

9.9

16.4

26.7

33.3

32.3

55.2

53.2

75.7

96.5

0.00 20.00 40.00 60.00 80.00 100.00

SweetAnn 

Monterey  

Radiance  

Albion  

Sabrina  

Grenada  

Sensation  

Winterstar  

Ventana

San Andreas 4.1

Festival 2.3

Portola 1.7

Safari 0.9

Fronteras 0.6

Petaluma 0.3

Percent Mortality on July 23, 2015

Monterey: 75%mortality

FUSARIUM TRIAL 2015

9.9

16.4

26.7

33.3

32.3

55.2

53.2

75.7

96.5

0.00 20.00 40.00 60.00 80.00 100.00

SweetAnn 

Monterey  

Radiance  

Albion  

Sabrina  

Grenada  

Sensation  

Winterstar  

Ventana

San Andreas 4.1

Festival 2.3

Portola 1.7

Safari 0.9

Fronteras 0.6

Petaluma 0.3

Percent Mortality on July 23, 2015

Albion: 53%mortality

FUSARIUM TRIAL 2015

9.9

16.4

26.7

33.3

32.3

55.2

53.2

75.7

96.5

0.00 20.00 40.00 60.00 80.00 100.00

SweetAnn 

Monterey  

Radiance  

Albion  

Sabrina  

Grenada  

Sensation  

Winterstar  

Ventana

San Andreas 4.1

Festival 2.3

Portola 1.7

Safari 0.9

Fronteras 0.6

Petaluma 0.3

Percent Mortality on July 23, 2015

San Andreas: 4%mortality

FUSARIUM TRIAL 2015

9.9

16.4

26.7

33.3

32.3

55.2

53.2

75.7

96.5

0.00 20.00 40.00 60.00 80.00 100.00

SweetAnn 

Monterey  

Radiance  

Albion  

Sabrina  

Grenada  

Sensation  

Winterstar  

Ventana

San Andreas 4.1

Festival 2.3

Portola 1.7

Safari 0.9

Fronteras 0.6

Petaluma 0.3

Percent Mortality on July 23, 2015

Portola: 2%mortality

FUSARIUM TRIAL 2015

0 1 1 2 2 410

16

2732 33

53 55

76

97

16

0 0 0 0 0

39

69

29

77

89

0

10

20

30

40

50

60

70

80

90

100

Fusarium wilt at MBA

2015 2016

% M

ortality

* * * *

* = not included in 2016 exp’t

www.calstrawberry.com

Conclusions•Wide range of susceptibility among genotypes and breeding program entries•High levels of resistance to Verticillium wilt, Fusariumwilt and Macrophomina crown rot•Results consistent between years•Aerial images were equally effective to ground ratings

Acknowledgements

SCRI grant No.2017‐51181‐26833

July 18, 2019

Hold the date: Field Day