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Expression analysis of human -secretase in transgenic tomato fruits Original Research Article

Protein Expression and Purification, Volume 82, Issue 1, March 2012, Pages 125-131

H.-S. Kim, J.-W. Youm, K.-B. Moon, J.-H. Ha, Y.-H. Kim, H. Joung, J.-H. Jeon

Abstract

An emerging strategy in biomanufacturing involves using transgenic plants to express recombinant pharmaceutical

and industrial proteins in large quantities. -Site APP cleaving enzyme 1 (-secretase 1, BACE1) is an enzyme

involved in the abnormal production of A42, the major component of senile plaques in Alzheimers disease (AD).

Thus, BACE1 represents a key target protein in the development of new potential drugs to treat Alzheimers disease.

We aimed to develop a tomato-derived recombinant BACE1 (rBACE1) protein to serve as a vaccine antigen that

would promote an immune response. We utilized a plant expression cassette, pE8BACE, to optimize BACE1

expression in tomato fruits. Polyemerase chain reaction and Southern blot analyses verified integration of the BACE1

gene into the plant genome. Northern and Western blot analyses demonstrated successful mRNA and protein

expression of rBACE1, respectively; the Sensizyme assay kit estimated the expression level of rBACE1 protein at

136 7 ng mg1

total soluble protein. The tomato-derived rBACE1 retains its activity for a long storage period at cool

or room temperature, and is highly resistant to degradation in conditions such as low acidity. Tomato-derived rBACE1

was severely degraded by heat or boiling. The proteolytic activity of tomato-derived rBACE1, confirmed by

fluorescence resonance transfer assay, was similar to that of a commercial sample ofEscherichia coli-derived

BACE1.

Tomat tahan dingin, disisipkan gen ikan flonder

Tanaman tahan kering,

Nyamuk yang membawa tetrasilin, yang mebuat larva matihttp://www.biosafety-info.net

Sebetulnya tujuan utama dari teknik breeding tradisional maupun bioteknologi

modern sama saja, yaitu bagaimana caranya agar dapat menyisipkan sebuah gen

atau beberapa gen dari sebuah organisme donor yang membawa atau memiliki

sifat-sifat baik yang dikehendaki ke dalam suatu organisme yang tidak

memiliki sifat-sifat (trait) tersebut.

Lebih dari 50 jenis tanaman pangan GMO telah lolos dari uji dan review

pemerintah federal AS dan sekitar 100 jenis komoditas GMO baru sedang

mengalami uji lapang.

Produk Problema Solusi Pemaparan

kondisi

Jagung bt Kematian pada hewan

non target

Penggunaan dari

pestisida jua

memberikan efek

yang lebih

buruk, bahkansecara langsung

maupun tidak

langsung maupun

tidak pada

lingkungan

Terjadi p

http://www.sciencedirect.com/science/article/pii/S1046592811003287http://www.sciencedirect.com/science/article/pii/S1046592811003287http://www.sciencedirect.com/science/article/pii/S1046592811003287http://www.biosafety-info.net/http://www.biosafety-info.net/http://www.sciencedirect.com/science/article/pii/S1046592811003287http://www.biosafety-info.net/

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