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European Conferences on

Biomedical Optics

Advanced Microscopy Techniques

Clinical and Biomedical Spectroscopy and Imaging

Diffuse Optical Imaging

Molecular Imaging

Novel Biophotonic Techniques and Applications

Optical Coherence Tomography and Coherence Techniques

Medical Laser Applications and Laser-Tissue Interactions

ICM—International Conference Centre Munich, Germany

22-26 May 2011

www.spie.org/ecbo

Cosponsored by:SPIE—The International Society

for Optical EngineeringOSA—Optical Society of America

Cooperating Organization:

Technical Programme

European Conferences on Biomedical Optics 2011 · www.spie.org/ecbo2

European Conferences on Biomedical Optics22–26 May 2011ICM—International Conference Centre Munich, Germany

Sponsored by

Cooperating Organisations

With support from

Air Force Office of Scientific Research

Student Award Sponsors

Welcome to Munich!The use of optical technologies and methods for biomedical applications

in diagnostics and therapeutics has emerged as a major research field. The

European Conferences on Biomedical Optics (ECBO) bring together scientists,

engineers, and clinicians from a variety of disciplines who are engaged in the

development and application of optical science and photonic technologies

to problems in biomedicine. The scope of this meeting will range from basic

research and instrumentation engineering to translational (bench-to-bedside)

research and clinical studies, with the common thread of employing optics

to impact problems in biology, medicine, or clinical health care. This biennial

meeting is jointly sponsored by SPIE and the Optical Society of America (OSA)

and will be co located with Laser World of Photonics 2011 and other society

meetings organized by WLT, EOS, SPIE Europe, EPS, OSA, and IEEE.

Program Chairs:

General Chairs:

Christoph K. Hizenberger,Medical University of Vienna (Austria)

Peter E. Andersen, Technical University of Denmark

Irene Georgakoudi, Tufts University (United States)

Brian W. Pogue,Dartmouth University (United States)


ContentsSpecial Events . . . . . . . . . . . . . . . . . . . . . . . . 6

Plenary Session . . . . . . . . . . . . . . . . . . . . . 7

Daily Events Schedule . . . . . . . . . . . . . . . . 8

General Information . . . . . . . . . . . . . . . . . . 8

Agenda of Sessions . . . . . . . . . . . . . . .9-10

Full Information: www.spie.org/ecboprogramme

Executive Organizing Committee

Stefan Andersson-Engels, Lund Univ. (Sweden)

Wolfgang Baeumler, Univ. Clinics Regensburg (Germany)

Jennifer K. Barton, The Univ. of Arizona (USA)

Emmanuel Beaurepaire, Ecole Polytechnique (France)

Niels Bendsoe, Lund Univ. Hospital (Sweden)

Andrew J. Berger, Univ. of Rochester (USA)

Claude A. Boccara, Ecole Supérieure de Physique et de Chimie Industrielles (France)

Stephen Allen Boppart, Univ. of Illinois at Urbana-Champaign (USA)

Brett E. Bouma, Massachusetts General Hospital (USA)

Ralf Brinkmann, Univ. zu Lübeck (Germany)

Paul J. Campagnola, Univ. of Connecticut Health Ctr. (USA)

Frank Chuang, Ctr. for Biophotonics Science and Technology (USA)

Johannes F. de Boer, Vrije Univ. Amsterdam (Netherlands)

Volker Deckert, Institut für Photonische Technologien (Germany)

Hamid Dehghani, The Univ. of Birmingham (United Kingdom)

Christian D. Depeursinge, Ecole Polytechnique Fédérale de Lausanne (Switzerland)

Kishan Dholakia, Univ. of St. Andrews (United Kingdom)

Wolfgang Drexler, Medizinische Univ. Wien (Austria)

Turgut Durduran, ICFO - Instituto de Ciencias Fotónicas (Spain)

Kevin W. Eliceiri, Univ. of Wisconsin-Madison (USA)

Robert Fedosejevs, Univ. of Alberta (Canada)

Paul M. French, Imperial College London (United Kingdom)

Martin Frenz, Univ. Bern (Switzerland)

James G. Fujimoto, Massachusetts Institute of Technology (USA)

Jesper Glückstad, Technical Univ. of Denmark (Denmark)

Christoph Haisch, Technische Univ. München (Germany)

Stefan W. Hell, Max-Planck-Institut für biophysikalische Chemie (Germany)

Raimund Hibst, Univ. Ulm (Germany)

Andreas H. Hielscher, Columbia Univ. (USA)

Robert A. Huber, Ludwig-Maximilians-Univ. München (Germany)

Xavier Intes, Rensselaer Polytechnic Institute (USA)

Karsten König, JenLab GmbH (Germany)

Theo Lasser, Ecole Polytechnique Fédérale de Lausanne (Switzerland)

Rainer A. Leitgeb, Medizinische Univ. Wien (Austria)

Adam Liebert, Institute of Biocybernetics and Biomedical Engineering (Poland)

Lothar D. Lilge, Ontario Cancer Institute (Canada)

Charles P. Lin, Wellman Ctr. for Photomedicine (USA)

Kristen Carlson Dawn Maitland, Texas A&M Univ. (USA)

Jerome Mertz, Boston Univ. (USA)

Vasilis Ntziachristos, Helmholtz Zentrum München GmbH (Germany)

Gregory M. Palmer, Duke Univ. (USA)

Francesco Saverio Pavone, LENS Lab., Univ. degli Studi di Firenze (Italy)

Carsten M. Philipp, Elisabeth Klinik (Germany)

Adrian Gh. Podoleanu, Univ. of Kent (United Kingdom)

Brian W. Pogue, Dartmouth College (USA)

Jürgen Popp, Institut für Photonische Technologien (Germany)

Nirmala Ramanujam, Duke Univ. (USA)

Lise L. Randeberg, Norwegian Univ. of Science and Technology (Norway)

Dominic J. Robinson, Univ. Medisch Ctr. Rotterdam (Netherlands)

Andrew M. Rollins, Case Western Reserve Univ. (USA)

Ricardas Rotomskis, Vilnius Univ. (Lithuania)

David D. Sampson, The Univ. of Western Australia (Australia)

Natalia M. Shakhova, Institute of Applied Physics (Russian Federation)

Melissa C. Skala, Duke Univ. (USA)

Peter T. C. So, Massachusetts Institute of Technology (USA)

Stefan B. Spaniol, CeramOptec GmbH (Germany)

Subhadra Srinivasan, Dartmouth College (USA)

Ronald Sroka, Ludwig-Maximilians-Univ. München (Germany)

Ernst H. K. Stelzer, European Molecular Biology Lab. (Germany)

Herbert Stepp, Ludwig-Maximilians-Univ. München (Germany)

Executive Organizing Committee

Hotel/Travel/Onsite Service Information

Hotel TravelOnsite ServicesFood and Beverage

Henricus J. C. M. Sterenborg, Erasmus MC (Netherlands)

Vinod Subramaniam, Univ. Twente (Netherlands)

Paola Taroni, Politecnico di Milano (Italy)

James Tunnel, The Univ. of Texas at Austin (USA)

Urs Utzinger, The Univ. of Arizona (USA)

Ton G. van Leeuwen, Academisch Medisch Centrum (Netherlands)

Gijs van Soest, Erasmus MC (Netherlands)

Rudolf M. Verdaasdonk, Vrije Univ. Medical Ctr. (Netherlands)

I. Alex Vitkin, Univ. of Toronto (Canada)

Alfred Vogel, Univ. zu Lübeck (Germany)

Heidrun Wabnitz, Physikalisch-Technische Bundesanstalt (Germany)

Alastair J. M. Watson, Royal Liverpool and Broadgreen Univ. Hospitals NHS Trust (United Kingdom)

Julia Welzel, General Hospital Augsburg (Germany)

Tony Wilson, Univ. of Oxford (United Kingdom)

Maciej Wojtkowski, Nicolaus Copernicus Univ. (Poland)

Martin Wolf, Univ. Hospital Zürich (Switzerland)

Yukio Yamada, The Univ. of Electro-Communications (Japan)

Yoshiaki Yasuno, Univ. of Tsukuba (Japan)

Bing Yu, Duke Univ. (USA)

Gang Zheng, Univ. of Toronto (Canada)

Technical Conferences

8086 Advanced Microscopy Techniques . . . . . . . . . . . . . . . . . 12

8087 Clinical and Biomedical Spectroscopy and Imaging . . . . 12

8088 Diffuse Optical Imaging . . . . . . . . 12

8089 Molecular Imaging III . . . . . . . . . . 12

8090 Novel Biophotonic Techniques and Applications . . . . . . . . . . . . . 13

8091 Optical Coherence Tomography and Coherence Techniques . . . . 13

8092 Medical Laser Applications and Laser-Tissue Interactions . . 13

Proceedings . . . . . . . . . . . . . . . . . . . . . . 66


Student AwardsWednesday 24 May . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10.30-10.45Room 11

The following student awards will be presented at a ceremony during the Post-Deadline session.

Best Student Paper and Best Student PosterSponsored by:

Best Student Paper/Poster (Developing Nation)Sponsored by:

Special Events

World of Photonics—Special EventsIncluded with your ECBO registration

Congress Opening and PlenaryMonday 23 May . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9.30 to 11.00Room 1, Ground Floor/1st Floor Congress Center

Laser World of Photonics Trade FairMonday 23-Wednesday 25 May . . . . . . . . . . . . . . . . . . . .9.00 to 17.00Thursday 26 May . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9.00 to 16.00

Munich Trade Fair Centre - Laser World of Photonics Get-Together ReceptionMonday 23 May . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .17.30 to 18.30Foyer, Ground Floor, Congress Centre

Join all Congress participants at this reception.

Poster SessionsICM Foyer Ground FloorMonday and Tuesday 23-24 May . . . . . . . . . . . . . . . . . .15.30 to 17.00Posters will be featured on Monday and Tuesday. Each day will repre-sent a different set of posters. Posters will be available during the lunch/poster viewing break. Poster sessions, with authors present, will be held Monday and Tuesday from 15.30 to 17.00. Coffee will be served.

Poster authors: Please set up posters on the morning of your session by noon, before the lunch/poster viewing break. You are required to stand by your poster during the poster session to discuss it with session at-tendees. Please remove your poster following the poster session. Posters left on the boards after the poster session concludes will be discarded.

ECBO Welcome ReceptionTuesday 24 May . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .19.30 to 21.00

Registered attendees are invited to participate in Reception at the Pau-laner Brewery in Munich. Guests of registered attendees may attend by purchasing tickets at the registration desk, €45 (space available).

Directions to Welcome Reception from ICMBy public transport: Take local train to Marienplatz, then change into U3/U6 in direction of Fuerstenried West or Klinikum Grosshadern. Get off at stop “Goetheplatz”. From there walk through Häberlstrasse; 2 mins on foot.

By car: Go towards city centre, railway station, middle ring, Sendlinger-Tor-Platz, Lindwurmstrasse to Goetheplatz, turn left into Häberlstrasse.

All Students & SPIE Fellows LuncheonMonday 23 May . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12:30 - 14:00

Students: Advance Sign-up Required On-site. Seating Limited. All students and SPIE Fellows are invited to this engaging networking opportunity. This event gives students an opportunity to network with SPIE Fellows who will share their insights into career paths in optics and photonics. Lunch is complimentary, but student sign-up at the ECBO registration desk is required.

Panel: Open Access in Scientific PublicationSunday 22 May . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16.30 to 18.00Room: 14C

Panel Chair: Frank Scholze, Head of KIT library (Karlsruhe Institute of Technology)

Organizers: Ulrike Küsters & Tina Klages, Competence-Center Fraunhofer-Online; Peter E. Andersen, Technical Univ. Denmark; Peter Loosen, Fraunhofer-ILT

Having entered the digital age, much owing to the widespread availability of the internet and the ability to reuse and distribute electronic data at low cost, the arguments for Open Access have gained new importance. Arguments for Open Access include information-sharing for the common good, improvement of visibility, and as a consequence the increase of citations. These arguments are currently debated among stakeholders in scientific publishing, including researchers, academics, librarians, university administrators, funding agencies, government officials, commercial publishers, and learned-society publishers. Although there is some agreement concerning the concept of Open Access, there is considerable debate about the economics of funding peer review in open-access publishing, and about the reliability and economic effects of self-archiving.

This panel discussion aims at shedding some light on these issues by first having short presentations from various stakeholders followed by a moderated debate.


14.10 to 14.50

Optical Spectroscopy for Clinical Detection of Pancreatic Cancer

Mary-Ann Mycek, University of Michigan (USA)

Abstract: Our research in Biomedical Optics develops tools to probe and quantify living biological systems, with a goal of impacting clinical care by creating non- and minimally-invasive optical diagnostic technologies. The translational “bench to bedside” research strategy we employ involves experimental investigations of live

cells, tissue-engineered constructs and human tissues, as well as com-putational studies with photon-tissue interaction models.

In the United States, pancreatic adenocarcinoma is the 4th leading cause of cancer death, with a 5-year survival rate of only 5%. Worldwide, the incidence and mortality rates for this disease are similar to the U.S. This dismal prognosis for pancreatic cancer patients is attributed mainly to the inability of current clinical methods to accurately detect the disease in its early stages of development. To investigate whether tissue optical spectroscopy could potentially aid in early diagnosis and improve survival rates, optical studies were conducted on human pancreatic tissues.

A prototype clinical fiber-optic probe coupled fluorescence and reflec-tance spectrometer was developed and employed in a pilot study to probe freshly excised human pancreatic tissues. Significant differences were observed in the optical responses of normal tissues, pancreatitis (inflammation), and adenocarcinoma. Quantitative photon-tissue inter-action models and tissue classification algorithms were developed and applied to successfully distinguish among these pancreatic tissue types. These studies suggest that multi-modal optical spectroscopy holds promise as a potential clinical method to differentiate among diseased and normal pancreatic tissues.

Biography: Mary-Ann Mycek, Ph.D., is an Associate Professor in the Bio-medical Engineering (BME) Department at the University of Michigan. She received her Ph.D. in Physics from U.C. Berkeley, where she specialized in condensed matter physics and optical spectroscopy, before pursuing postdoctoral training in laser medicine at Massachusetts General Hos-pital and Harvard Medical School. As an Assistant Professor of Physics at Dartmouth, she established an experimental and computational re-search group in biomedical optics, a multidisciplinary field incorporating elements of the physical and life sciences, engineering, and medicine. At the University of Michigan, she joined the College of Engineering as an Associate Professor of BME and established the Biomedical Optics Laser Laboratory. Her translational research program includes basic (pre-clinical), applied (clinical), and computational research toward quan-titative, non-invasive, optical sensing and imaging in cells and tissues. Recently, she served as an Associate Chair for the BME Department and in this capacity was Director of the U-M Graduate Program in BME.

ECBO Plenary Session Tuesday 24 May 14.00-15.30 • Room 5

14.50 to 15.30

Twenty Years of Optical Coherence Tomography: Where is it heading?

Wolfgang Drexler, Medizinische Univ. Wien ( Austria)

Abstract: In the last two decades optical coherence tomography (OCT) has established itself as a unique non-invasive, optical medical diagnostic imaging modality, enabling unprecedented in vivo cross-sectional tomographic visualization of internal microstructure in a variety of biological systems. Ophthalmology has been

the most successful and commercially most active medical field for OCT so far, but several other OCT applications, e.g., in cardiology, dentistry, gastroenterology or dermatology, are on the verge of expanding their market comparable to or larger than that of ophthalmology.

Especially in the last decade, ultrabroad bandwidth light sources as well as spectral/frequency domain OCT detection technology enabled three-dimensional ultrahigh resolution OCT with unprecedented axial resolution, approaching resolution levels of conventional histopathol-ogy, enabling optical biopsy of biological tissue. Furthermore emerging swept source laser technologies and parallel or full-field OCT techniques enabled multiple millions of A-scan rates per second, allowing large area OCT scans with high definition sampling, investigation of dynamic processes or four-dimensional (3D over time) imaging.

In addition, extensions of OCT are under development that should provide enhanced contrast or non-invasive depth resolved functional imaging of the investigated tissue, including extraction of birefringent, spectroscopic, blood flow or physiologic tissue information. These extensions of OCT should not only improve image contrast, but should also enable the differentiation and early detection of pathologies via localized functional state.

Recently OCT has also been combined with different complementary imaging technologies (photoacoustics, CARS, multi-photon microscopy, fluorescent imaging) to hybrid/multi-modal approaches to compensate fundamental limits of OCT in order to significantly enhance its perfor-mance towards molecular imaging.

Biography: Wolfgang Drexler received his MS and PhD in Electrical Engineering in 1991 and 1995, respectively, at the Technical Univer-sity of Vienna, Austria. From 2006 to 2009 he was a Full Professor of Biomedical Imaging at the School of Optometry and Vision Sciences at Cardiff University, Wales, UK. Since October 2009 he is a Full Professor of Medical Physics and the Head of the Center for Medical Physics and Biomedical Engineering at the Medical University of Vienna, Austria and is also Director of the Christian Doppler Laboratory for Laser Development and their Application in Medicine since 2011. He spent two years at the Massachusetts Institute of Technology (MIT), Cambridge, USA, received the Austrian START Award from the Austrian Science Fund in 2001, the COGAN Award from ARVO in 2007, the Fear Memorial Award in 2009, the Gabriel Coscas Medal and the EVER Acta Silver Medal in 2010. He is a member of the Austrian Academy of Science and has published more than 120 papers in peer reviewed journals.

Special Events

14.00 to 14.10

Welcome Remarks Peter Andersen, Technical Univ. of Denmark; Irene Georgakoudi, Tufts Univ. (USA)


Conf. 8086 Advanced Microscopy Techniques (So, Beaurepaire) p 14

Conferences

Sunday Monday Tuesday Wednesday Thursday

Special Events

Conf. 8087 Clinical and Biomedical Spectroscopy and Imaging (Ramanujam, Popp) p 13

Conf. 8088 Diffuse Optical Imaging (Hielscher, Taroni) p 18

Conf. 8089 Molecular Imaging (Lin, Ntziachristos) p 22

Conf. 8090 Novel Biophotonic Techniques and Applications (Sterenborg, Vitkin) p 24

Conf. 8091 Optical Coherence Tomography and Coherence Techniques (Leitgeb, Bouma) p 26

Conf 8092 Medical Laser Applications and Laser-Tissue Interactions (Sroka, Lilge) p 30

Joint session with E-CLEO, JS1 (conf. 8091) p 26

Poster Sessions p 28

Joint session with E-CLEO, JS2 (conf. 8086 and 8090) p 34

ECBO Plenary Session p 7

Poster Session p 38

Welcome Reception p 6

Post-Deadline Session and Student Award Presentations p 46

World of Photonics Opening/Plenary p 6

Daily Schedule

General InformationRegistration Hours:Saturday . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15.30 to 18.00Sunday . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.30 to 17.30Monday–Thursday . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.00-17.30

Coffee BreaksGround Level FoyerSunday . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17.00 to 17.30Monday . . . . . . . . . . . . . . . . . . . 10.00 to 10.30 and 16.00 to 16.30Tuesday . . . . . . . . . . . . . . . . . . . 10.00 to 10.30 and 16.00 to 16.30Wednesday-Thursday . . . . . . . 10.00 to 10.30; and 16.00 to 16.30

InternetWi-fi access for laptop users and a number of complimentary computer stations, with timed access can be found in the ICM Foyer areas.

Audiovisual EquipmentThe presentation mode is that of a preload mechanism run by the company m-Events in Munich, Germany. Authors will be contacted by this company with exact instructions on how do upload their presentations and e-posters. The meeting rooms will contain the relevant equipment to carry out a centralised screening process. Any questions regarding compatibility would need to be directed to m-Events.

Panel: Open Access in Scientific Publication p 6


Agenda of Sessions - Monday 23 MaySession times may vary. Please check conference programs for exact timing.

Conference Abbreviations Key —AMT—Advanced Microscopy Techniques (8086)—CBS—Clinical and Biomedical Spectroscopy and Imaging (8087)—DOI—Diffuse Optical Imaging (8088)—MI—Molecular Imaging (8089)—NBTA—Novel Biophotonic Techniques and Applications (8090)—OCT—Optical Coherence Tomography and Coherence Techniques (8091)—TLA—Medical Laser Applications and Laser-Tissue Interactions (8092)

Agenda of Sessions - Sunday 22 MaySession times may vary. Please check conference programs for exact timing.

Room 5 Room 11 Room 2 Room 3

9.00-10.30 —AMT—Nonlinear Microscopy:

2PEF

—DOI—Mapping of Brain Function with DOI and Multimodality

Imaging

7.00-9.00 Registration, ICM Entry Lobby

10.30-11.00 Coffee Break, Ground Floor Congress Centre

11.00-13.00 —AMT— Phase and Holographic

Microscopy

—DOI—New Methods and

Techniques for Brain Imaging

—MI—Molecular Optoacoustic

Imaging

13.00-14.30 Lunch Break

14.30-16.00 —AMT—Molecular Imaging and

Novel Geometries I

—DOI—Human Head Models

—MI—Novel Techniques for

Molecular Visualization

—NBTA—Novel Imaging Approaches


16.30-18.00 —AMT—Nonlinear Microscopy: SHG, THG, Multimodal

—DOI—Modeling of Light

Propagation

—MI—15.30 start

Optical and Optoacoustic Probes and Labeling

—NBTA—17.00 start

Emerging Technologies: Models and Platforms

—AMT— Nonlinear Microscopy: CARS,

FWM, Pulse Shaping, Technology

—DOI— Optical Tomography I

—NBTA— Emerging Technologies: Models and Platforms II

12.45-14.00 Lunch Break and Poster Viewing

14.00-15.30 —AMT— Molecular Imaging and Novel

Geometries II

—DOI— Optical Tomography II

—NBTA— Photoacoustic Techniques

15.30-17.00 Coffee Break and Poster Session, Ground Floor/Foyer Congress Centre

17.00-18.00 —AMT— Embryo Imaging

—DOI— Optical Tomography III

Room 5 Room 11 Room B13

7.00-9.30 Registration, ICM Entry Lobby

9.30-11.00 World of Photonics Congress Opening/Plenary

11.00-11.15 Room Change

11.15-12.45


Agenda of Sessions - Wednesday 25 MaySession times may vary. Please check conference programs for exact timing.

Agenda of Sessions - Thursday 26 MaySession times may vary. Please check conference programs for exact timing.

—CBS— Clinical and Preclinical Tissue

Characterization I


08.30 to 10.30 —TLA— Nanoparticle and NS Laser

Applications

—OCT— Polarization-Sensitive OCT


Characterization II

10.30 to 12.30 —TLA— Post-Deadline Session and

Student Awards

—OCT— Microcirculation Imaging

14.00 to 16.00



Characterization III

—TLA— Photodynamic Investigation

—OCT— Advanced Data Processing and

Signal Enhancement

16.00 to 18.00 —CBS— Clinical and Preclinical Tissue

Characterization IV

—TLA— Laser-Assisted Detection

Techniques

—OCT— Technological Advances




08.30 to 10.30 —CBS— Skin Diagnostics and Therapy I

—TLA— Biomodulation and Blood

—OCT— Intravascular and Endoscopic

OCT

—CBS— Skin Diagnostics and Therapy II

10.30 to 11.30 —TLA— Clinical Laser Applications

—OCT— Biomedical Applications of OCT

—CBS— Clinical and Preclinical

Diagnostics I

11.30 to 12.30

14.00 to 15.30 —CBS— Clinical and Preclinical

Diagnostics II

—TLA— Lasers in Ophthalmology

—OCT— Ophthalmic OCT Techniques

16.00 to 18.00 —CBS— Clinical and Preclinical

Diagnostics III

—TLA— FS Laser Applications

—OCT— Advanced Coherent Sensing and

Imaging Concepts II




ECBO Plenary Session

Posters, ICM Foyer Ground Floor

Agenda of Sessions - Tuesday 24 MaySession times may vary. Please check conference programs for exact timing.

12.30-14.00 Lunch Break and Poster Viewing

—AMT—Advanced Biophotonics: Sensing and Imaging: Joint Session with

E-CLEO


08.30 to 10.00

10.30 to 11.15

11.15 to 12.30

—CBS—Minimally Invasive Diagnostics/

Laboratory Medicine I

—DOI—Start at 08.45

Novel Instrumentation

—CBS—Minimally Invasive Diagnostics/

Laboratory Medicine II

—DOI—Experimental Methods

—OCT—Advanced Coherent Sensing

and Imaging Concepts I

—CBS—Biospectroscopy and POC

Diagnostics I

14.00 to 15.30

15.30 to 17.00

—CBS—Biospectroscopy and POC

Diagnostics II

17.00 to 18.00 —DOI—Clinical Applications


—OCT—Optical Coherence Microscopy

—OCT—Poster Previews


ECBO Plenary Speakers8087-501 Twenty years of optical coherence tomography: where

is it heading?, Wolfgang Drexler, Medizinische Univ. Wien (Austria)

8087-502 Optical spectroscopy for clinical detection of pancre-atic cancer, Mary-Ann Mycek, Univ. of Michigan (USA)

ECBO/E-CLEO Joint Session (JS1): Medical Imaging8091-500 Label-free live brain imaging with third-harmonic gen-

eration microscopy, Stefan Witte, Vrije Univ. Amsterdam (Netherlands)

8091-501 Optical coherence tomography imaging: technology and applications, James Fujimoto, Massachusetts Insti-tute of Technology (USA)

ECBO/E-CLEO Joint Session (JS2): Advanced Biophotonics: Sensing and Imaging8090-45 Nonlinear microscopy of tissues and embryo morpho-

genesis, Emmanuel Beaurepaire, Ecole Polytechnique (France)

8090-46 Silk - new opportunities in optics and photonics for an ancient material, Fiorenzo Omenetto, Tufts Univ. (USA)

8090-47 Development of transient absorption ultrasonic mi-croscopy, Brian Applegate, Texas A&M Univ. (USA)

8086 Advanced Microscopy Techniques II8086-1 Dual-beam two-photon microscopy for simultaneous

fast acquisition of two focal planes, Lauren Grosberg, Columbia Univ. (USA)

8086-6 Quantitative phase imaging-based refractive index determination of living cells using incorporated microspheres as reference, Björn KemperWestfälische Wilhelms-Univ. Münster (Germany)

8086-13 Towards in vivo tomographic FLIM-FRET imaging of biomolecular interactions, James McGinty Imperial Col-lege London (United Kingdom)

8086-18 Optical recording of action potential propagation in t-tubular network, Leonardo Sacconi Univ. degli Studi di Firenze (Italy)

8086-23 Accessing nonlinear phase contrast in biological tis-sue using femtosecond laser pulse shaping, Martin Fischer, Duke Univ. (USA)

8086-28 3D tumor imaging by self interference fluorescence endoscopy, Mattijs de Groot Vrije Univ. Amsterdam (Netherlands)

8086-33 Light sheet-based fluorescence microscopy (LSFM) reduces phototoxic effects and provides new means for the modern life sciences, Ernst H. Stelzer, European Molecular Biology Lab. (Germany)

8086-34 Cell lineage reconstruction of early zebrafish embryos using multiharmonic microscopy, Emmanuel Beaure-paire, Ecole Polytechnique (France)

8086-35 Imaging embryos with multiphoton light sheet micros-copy, Willy Supatto, California Institute of Technology (USA), Ecole Polytechnique (France)

8087 Clinical and Biomedical Spectroscopy and Imaging II8087-1 Molecular histopathology by nonlinear interferometric

vibrational imaging, Stephen Boppart, Univ. of Illinois at Urbana-Champaign (USA)

8087-5 Bacterial identification in real samples by means of micro-Raman spectroscopy, Petra Rösch, Friedrich-Schiller-Univ. Jena (Germany)

8087-12 Collagen matrices as a more suitable model for in vitro study of live cells using Raman microspectros-copy, Franck Bonnier Dublin Institute of Technology (Ireland)

8087-19 Quantitative spectral imaging for intraoperative breast tumor margin assessment, Bing Yu , Duke Univ. (USA)

8087-26 Nano-sensitizers for multi-modality optical diagnostic imaging and therapy of cancer, Malini Olivo, National Univ. of Ireland, Galway, Royal College of Surgeons (Ire-land), National Univ. of Singapore (Ireland)

Invited Speakers

8087-38 Diagnostics and treatment of tumours using laser techniques, Katarina Svanberg, Lund Univ. Hospital (Sweden)

8087-42 Differentiation of eumelanin and pheomelanin in skin lesions using transient absorption microscopy, Martin Fischer, Duke Univ. (USA)

8087-45 Toward clinically relevant speeds and depth in 3D mi-crocirculation imaging, Martin Leahy, Univ. of Limerick (Ireland)

8087-47 Novel fiber tip sensor for pH measurement in gastro-esophageal apparatus, Francesco Baldini, Istituto di Fisica Applicata Nello Carrara (Italy)

8088 Diffuse Optical Imaging III8088-1 Assessment of cortical response during motor task in

adults by a multimodality approach based on fNIRS-EEG, fMRI-EEG, and TMS, Alessandro Torricelli, Politec-nico di Milano (Italy)

8088-25 Compressive diffuse optical tomography, Turgut Dur-duran, ICFO - Instituto de Ciencias Fotónicas (Spain)

8088-36 NIRFAST: An interactive GUI driven toolbox for model-ling and image reconstruction in optical imaging, Ha-mid Dehghani,The Univ. of Birmingham (United Kingdom)

8088-37 3D near-infrared imaging based on a single-photon avalanche diode array sensor, Juan Mata Pavia, Univ. Hospital Zürich (Switzerland), Ecole Polytechnique Fé-dérale de Lausanne (France)

8089 Molecular Imaging III8089-1 Optoacoustic tomography of cancer receptors in

mouse models using targeted gold nanorod conju-gates, Alexander Oraevsky, TomoWave Labs., Inc. (USA)

8089-13 Receptor mediated aggregation of plasmonic nanoparticles in optical and photoacoustic molecular imaging of cancer, Konstantin Sokolov, The Univ. of Texas M.D. Anderson Cancer Ctr. (USA)

8091 Optical Coherence Tomography and Coherence Techniques V8091-1 High-speed functional OCT with self-reconstructive

Bessel illumination at 1300nm, Cedric Blatter, Med-izinische Univ. Wien (Austria)

8091-4 Combination of dark-field optical coherence micros-copy with epi-fluorescence microscopy for functional cell imaging, Christophe Pache, Ecole Polytechnique Fédérale de Lausanne (Switzerland)

8091-13 Double-beam Doppler optical coherence tomography for visualizing the microvasculature within the human retina, Stefan Zotter, Medizinische Univ. Wien (Austria)

8091-14 High speed and high penetration Doppler optical coherence tomography, Young-Joo Hong, Univ. of Tsukuba (Japan)

8091-24 Megahertz retinal OCT: advanced data processing protocols enabled by densely sampled ultrawide-field data, Thomas Klein, Ludwig-Maximilians-Univ. München (Germany)

8091-45 Simultaneous, tracked multi-wavelength optical coherence tomography for clinical applications, Boris Pova?ay, Medizinische Univ. Wien (United Kingdom)

8092 Medical Laser Applications and Laser-Tissue Interactions V8092-13 Sentinel lymph node detection by an optical method

using scattered photons, Franklin Tellier, Univ. de Stras-bourg (France)

8092-40 High-power, diode-pumped Er:YAG lasers for soft and hard tissue applications, Arne Heinrich, Pantec Biosolu-tions AG (Liechtenstein)


8086Room: 5

Sunday-Monday 22-23 May 2011 Proceedings of SPIE Vol. 8086

Advanced Microscopy

Techniques IIConference Chairs: Peter T. C. So, Massachusetts

Institute of Technology (USA); Emmanuel Beaurepaire, Ecole

Polytechnique (France)

Programme Committee: Claude A. Boccara, Ecole

Supérieure de Physique et de Chimie Industrielles (France);

Paul J. Campagnola, Univ. of Connecticut Health Ctr. (USA);

Christian D. Depeursinge, Ecole Polytechnique Fédérale

de Lausanne (Switzerland); Kishan Dholakia, Univ. of St. Andrews (United Kingdom); Kevin W. Eliceiri, Univ. of

Wisconsin-Madison (USA); Paul M. French, Imperial College

London (United Kingdom); Irene Georgakoudi, Tufts Univ. (USA);

Jesper Glückstad, Technical Univ. of Denmark (Denmark); Stefan W. Hell, Max-Planck-Institut für biophysikalische Chemie (Germany); Karsten

König, JenLab GmbH (Germany); Charles P. Lin, Wellman Ctr. for Photomedicine (USA); Jerome

Mertz, Boston Univ. (USA); Francesco Saverio Pavone, Univ. degli Studi di Firenze (Italy); Ernst H. K. Stelzer,

European Molecular Biology Lab. (Germany); Vinod Subramaniam, Univ. Twente (Netherlands); Tony Wilson, Univ. of Oxford (United

Kingdom)

Conference continues on page 14

8087Room: B13

Tuesday-Thursday 24-26 May 2011 Proceedings of SPIE Vol. 8087

Clinical and Biomedical

Spectroscopy and Imaging II

Conference Chairs: Nirmala Ramanujam, Duke Univ.

(USA); Jürgen Popp, Institut für Photonische Technologien

(Germany)

Programme Committee: Niels Bendsoe, Lund Univ. Hospital (Sweden); Andrew J. Berger,

Univ. of Rochester (USA); Volker Deckert, Institut für Photonische Technologien

(Germany); Kishan Dholakia, Univ. of St. Andrews (United Kingdom); Kristen Carlson Dawn Maitland, Texas A&M

Univ. (USA); Gregory M. Palmer, Duke Univ. (USA); Francesco Saverio Pavone, LENS Lab.,

Univ. degli Studi di Firenze (Italy); Lise L. Randeberg, Norwegian

Univ. of Science and Technology (Norway); Melissa C. Skala,

Duke Univ. (USA); James Tunnel, The Univ. of Texas at Austin

(USA); Urs Utzinger, The Univ. of Arizona (USA); Alastair J. M. Watson, Royal Liverpool and

Broadgreen Univ. Hospitals NHS Trust (United Kingdom); Bing Yu,

Duke Univ. (USA)


8088Room: 11

Sunday-Tuesday 22-24 May 2011 Proceedings of SPIE Vol. 8088

Diffuse Optical Imaging III

Conference Chairs: Andreas H. Hielscher, Columbia Univ. (USA);

Paola Taroni, Politecnico di Milano (Italy)

Programme Committee: Hamid Dehghani, The Univ. of Birmingham (United Kingdom);

Turgut Durduran, ICFO - Instituto de Ciencias Fotónicas

(Spain); Xavier Intes, Rensselaer Polytechnic Institute (USA); Adam Liebert, Institute of

Biocybernetics and Biomedical Engineering (Poland); Subhadra Srinivasan, Dartmouth College

(USA); Heidrun Wabnitz, Physikalisch-Technische

Bundesanstalt (Germany); Martin Wolf, Univ. Hospital Zürich

(Switzerland); Yukio Yamada, The Univ. of Electro-Communications

(Japan)


8089Room: 2

Sunday 22 May 2011 Proceedings of SPIE Vol. 8089

Molecular Imaging III

Conference Chairs: Charles P. Lin, Wellman Ctr. for

Photomedicine (USA); Vasilis Ntziachristos, Technische

Univ. München (Germany) and Helmholtz Zentrum München

GmbH (Germany

Programme Committee: Fabian Kiessling, RWTH Aachen

(Germany); Jan Grimm, Memorial Sloan-Kettering Cancer Ctr.

(USA); Clemens W.G.M. Lowik, Leiden Univ. (Netherlands);

Gooitzen M. van Dam, Univ. Medical Ctr. Groningen

(Netherlands); D. Michael Olive, LI-COR Biosciences

(USA); Alexander A. Oraevsky, TomoWave Labs., Inc. (USA); Gang Zheng, Univ. of Toronto

(Canada); Nikolaos C. Deliolanis, Helmholtz Zentrum München

GmbH (Germany)




8090Room: 3

Sunday-Tuesday 22-24 May 2011 Proceedings of SPIE Vol. 8090

Novel Biophotonic

Techniques and Applications

Conference Chairs: Henricus J. C. M. Sterenborg, Erasmus MC (Netherlands); Alex Vitkin, Univ.

of Toronto (Canada)

Programme Committee: Arjen Amelink, Erasmus MC

(Netherlands); Vanderlei Salvador Bagnato, Univ. de São Paulo (Brazil); Paul C. Beard, Univ.

College London (United Kingdom); Daniel Cote, Ctr. de Recherche

de l’Univ. Laval Robert-Giffard (Canada); Stanislav Y. Emelianov, The Univ. of

Texas at Austin (USA); Dirk J. Faber, Univ. van Amsterdam

(Netherlands); Venkataramanan Krishnaswamy, Dartmouth

College (USA); Steen J. Madsen, Univ. of Nevada, Las Vegas (USA); Igor Meglinski, Univ. of Otago (New Zealand);

Seemantini K. Nadkarni, Harvard Medical School (USA); Günther

Paltauf, Karl-Franzens-Univ. Graz (Austria); Gijs van Soest,

Erasmus MC (Netherlands); Chris Xu, Cornell Univ. (USA)


8091

Room: 13B Tuesday-Thursday

24-26 May 2011 Proceedings of SPIE Vol. 8091

Optical Coherence

Tomography and Coherence Techniques V

Conference Chairs: Rainer A. Leitgeb, Medizinische Univ.

Wien (Austria); Brett E. Bouma, Massachusetts General Hospital

(USA)

Programme Committee: Jennifer K. Barton, The Univ.

of Arizona (USA); Stephen Allen Boppart, Univ. of Illinois at Urbana-Champaign (USA); Johannes F. de Boer, Vrije

Univ. Amsterdam (Netherlands); Wolfgang Drexler, Medizinische

Univ. Wien (Austria); James G. Fujimoto, Massachusetts Institute of Technology (USA);

Robert A. Huber, Ludwig-Maximilians-Univ. München

(Germany); Theo Lasser, Ecole Polytechnique Fédérale de

Lausanne (Switzerland); Adrian Gh. Podoleanu, Univ. of Kent

(United Kingdom); Andrew M. Rollins, Case Western

Reserve Univ. (USA); David D. Sampson, The Univ. of

Western Australia (Australia); Natalia M. Shakhova, Institute

of Applied Physics (Russian Federation); Gijs van Soest,

Erasmus MC (Netherlands); Ton G. van Leeuwen, Academisch

Medisch Centrum (Netherlands); Julia Welzel, General Hospital Augsburg (Germany); Maciej

Wojtkowski, Nicolaus Copernicus Univ. (Poland); Yoshiaki Yasuno,

Univ. of Tsukuba (Japan)


8092Room: 11

Tuesday-Thursday 24-26 May 2011 Proceedings of SPIE Vol. 8092

Medical Laser Applications and

Laser-Tissue Interactions V

Conference Chairs: Ronald Sroka, Ludwig-Maximilians-Univ. München (Germany); Lothar D. Lilge, Ontario Cancer Institute

(Canada)

Programme Committee: Stefan Andersson-Engels, Lund Univ. (Sweden); Wolfgang Baeumler,

Univ. Clinics Regensburg (Germany); Ralf Brinkmann, Univ. zu Lübeck (Germany);

Santiago Camacho-López, Ctr. de Investigación Científica y de

Educación Superior de Ensenada (Mexico); Frank Chuang, Ctr. for Biophotonics Science and

Technology (USA); Robert Fedosejevs, Univ. of Alberta (Canada); Martin Frenz, Univ. Bern (Switzerland); Christoph

Haisch, Technische Univ. München (Germany); Raimund

Hibst, Univ. Ulm (Germany); Carsten M. Philipp, Elisabeth

Klinik (Germany); Brian W. Pogue, Dartmouth College

(USA); Dominic J. Robinson, Univ. Medisch Ctr. Rotterdam

(Netherlands); Ricardas Rotomskis, Vilnius Univ.

(Lithuania); Stefan B. Spaniol, CeramOptec GmbH (Germany);

Herbert Stepp, Ludwig-Maximilians-Univ. München

(Germany); Alfred Vogel, Univ. zu Lübeck (Germany); Rudolf M. Verdaasdonk, Vrije Univ. Medical Ctr. (Netherlands); Gang Zheng,

Univ. of Toronto (Canada)




SESSION 1

Room: 5 Sun. 09.00 to 10.30

Nonlinear Microscopy: 2PEF

Session Chair: Francesco Saverio Pavone,

Univ. degli Studi di Firenze (Italy)

SESSION 1

Room: 11 Sun. 09.00 to 10.30

Mapping of Brain Function with

DOI and Multimodality Imaging

Session Chairs: Heidrun Wabnitz, Physikalisch-Technische Bundesanstalt (Germany);

Yukio Yamada, The Univ. of Electro-Communications (Japan)

09.00: Assessment of cortical response during motor task in adults by a multimodality approach based on fNIRS-EEG, fMRI-EEG, and TMS (Invited Paper), Alessandro Torricelli, Davide Contini, Matteo Caffini, Lucia Zucchelli, Rinaldo Cubeddu, Politecnico di Milano (Italy); Lorenzo Spinelli, Istituto di Fotonica e Nanotecnologie (Italy); Erika Molteni, Anna M. Bianchi, Giuseppe Baselli, Sergio Cerutti, Politecnico di Milano (Italy); Elisa Visani, Isabella Giglioli, Davide Rossi Sebastiano, Elena Schiaffi, Ferruccio Panzica, Silvana Franceschetti, Fondazione I.R.C.C.S. Istituto Neurologico Carlo Besta (Italy) . . . . . . . . . . . . . . . . . . . [8088-01]A multimodality approach based on fNIRS-EEG, fMRI-EEG and TMS was used on adult volunteers during motor task aiming at optimizing a functional imaging procedure to be eventually used on patients with movement disorders.

09.30: Activated brain or superficial vein? the physiological origin of systemic artefacts in functional near infrared spectroscopy, Evgeniya P. Kirilina, Freie Univ. Berlin (Germany); Alexander Jelzow, Physikalisch-Technische Bundesanstalt (Germany); Angela Heine, Michael Niessing, Arthur Jacobs, Freie Univ. Berlin (Germany); Heidrun Wabnitz, Rainer Macdonald, Rüdiger Brühl, Bernd Ittermann, Physikalisch-Technische Bundesanstalt (Germany); Ilias Tachtsidis, Univ. College London (United Kingdom) . . . . . . . . . . . . . . . . [8088-02]We applied time-domain fNIRS, fMRI, MR-angiography and per ipheral physiological measurements to explore the physiological origin of task-evoked superficial artefacts in fNIRS. By temporal and spatial comparison of multimodal data we demonstrate that systemic signal in fNIRS originate from veins draining the scalp.

8086: Room 5Advanced Microscopy Techniques II

8088: Room 11Diffuse Optical Imaging III

09.00: Dual-beam two-photon microscopy for simultaneous fast acquisition of two focal planes (Invited Paper), Lauren Grosberg, Elizabeth M. C. Hillman, Columbia Univ. (USA) . . . . . . . . . . . . . . . . . . . [8086-01]The speed of three dimensional (3D) image acquisit ion in two-photon microscopy is inherently limited because a laser must be scanned over an entire volume to generate data. We present an imaging scheme for fast imaging that uses two laser beams focused in different planes to sample a 3D region.

09.30: Open-loop wavefront sensing scheme for specimen aberrations correction in two-photon excited fluorescence microscopy, Rodrigo A. Aviles-Espinosa, ICFO - Instituto de Ciencias Fotónicas (Spain); Jordi Andilla, Rafael Porcar-Guezenec, Xavier Levecq, Imagine Optic SA (France); David Artigas, ICFO - Instituto de Ciencias Fotónicas (Spain) and Univ. Politècnica de Catalunya (Spain); Pablo Loza-Alvarez, ICFO - Instituto de Ciencias Fotónicas (Spain) . . [8086-02]The wavefront emitted form a nonlinear fluorescence focal volume, excited inside a sample, is measured using a Shack-Hartmann wavefront sensor. This allows recording the aberrations captured by that point in a single measurement. Aberration correction is then implemented in an open-loop configuration (“single-shot”) by properly shaping a calibrated deformable mirror.

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SESSION 1 continued09.45: High-resolution imaging of nestin-expressing stem cells in hair follicles of mice, Aisada A. Uchugonova, Karsten Koenig, Univ. des Saarlandes (Germany); Michelle A. Digman, Enrico Gratton, Univ. of California, Irvine (USA); Jennifer Duong, Robert M. Hoffman, AntiCancer, Inc. (USA) . . . . . . . . . . . . . . . . . . . [8086-03]Nestin-expressing stem cells in whisker follicles and in hair follicles of live mice have been imaged using confocal two-photon microscopy, spectral imaging, FLIM, and multiphoton tomography. We found that the bulge and the dermal papilla of the hair follicle are the two major sources of nestin-expressing multipotent stem cells with similar properties.

SESSION 1 continued09.45: Simultaneous measurement of time-domain fNIRS and physiological signals during a cognitive task, Alexander Jelzow, Physikalisch-Technische Bundesanstalt (Germany); Ilias Tachtsidis, Univ. College London (United Kingdom); Evgeniya Kirilina, Michael Niessing, Freie Univ. Berlin (Germany); Ruediger Bruehl, Heidrun Wabnitz, Physikalisch-Technische Bundesanstalt (Germany); Angela Heine, Freie Univ. Berlin (Germany); Bernd Ittermann, Rainer Macdonald, Physikalisch-Technische Bundesanstalt (Germany) . . . . . . . . . . . . . . . [8088-03]We present simultaneous recordings of time-domain fNIRS and systemic physiological signals (heart rate, blood pressure, respiration, galvanic skin response, scalp blood flow, etc.) performed on the frontal lobe of 15 subjects during a continuous word performance task. Temporal behavior and mutual correlations between systemic data and optical signals were investigated.

10.00: Noninvasive evaluation of cerebral hemodynamics during Acetazolamide challenge: a diffuse optics and transcranial Doppler study, Peyman Zirak, ICFO - Instituto de Ciencias Fotónicas (Spain); Raquel Delgado-Mederos, Lavinia Dinia, Joan Martí-Fàbregas, Hospital de la Santa Creu i Sant Pau (Spain); Turgut Durduran, ICFO - Instituto de Ciencias Fotónicas (Spain) . . . . . . . . . [8088-04]A combined diffuse-optics/Doppler-ultrasonography technique is used to follow the hemodynamic changes after Acetazolamide(ACZ) bolus, on healthy controls and in patients with severe carotid artery steno-occlusive lesions. The micro- and macro-vascular cerebral-vasomotor-reactivity(CVR) values showed good agreement over all studied subjects.

10.15: Very low frequency cortical hemodynamic oscillations during slow wave sleep measured with near-infrared spectroscopy, Jaakko Virtanen, Tiina Näsi, Aalto Univ. School of Science and Technology (Finland) . . . . . . . . . . . . . . . . . [8088-05]Near-infrared spectroscopy (NIRS) can be used to monitor cortical hemodynamic changes during sleep. In a series of 30 all-night NIRS-electroencephalography (EEG) sleep measurements, we found particularly slow-wave sleep to be characterized by cortical hemodynamic oscillations centered at approximately 0.008 Hz.

8086: Room 5Advanced Microscopy Techniques II

8088: Room 11Diffuse Optical Imaging III

10.00: Two-photon excited fluorescence microscopy application for ex vivo investigation of ocular fundus samples, Sven Peters, Martin Hammer, Dietrich Schweitzer, Friedrich-Schiller-Univ. Jena (Germany) [8086-04]Two-photon excited fluorescence imaging has been employed to more precisely character ize the autof luorescence proper t ies o f endogenous fluorophores of individual anatomical structures in porcine ocular fundus ex vivo samples compared to present in vivo approaches.

10.15: Non-invasive quantitative assessments of engineered adipose tissue development using multi-photon excited fluorescence microscopy, Kyle P. Quinn, Nikolaos Fourligas, Evangelia Bellas, David L. Kaplan, Irene Georgakoudi, Tufts Univ. (USA) . . . . . . . . . . . . . . . . . . . [8086-05]Multi-photon excited fluorescence microscopy was utilized to monitor biochemical changes associated with the development of silk-based engineered constructs into functional adipose tissue. Based on the intrinsic fluorescence of NADH and FAD, cells within vascularized adipose tissue demonstrated significantly higher metabolic activity than cells in non-vascularized control tissue.

Coffee Break . . . . . . . . .10.30 to 11.00 Coffee Break . . . . . . . . .10.30 to 11.15

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SESSION 1

Room: 2 Sun. 11.00 to 13.00

Molecular Optoacoustic Imaging

Session Chair: Vasilis Ntziachristos, Helmholtz Zentrum

München GmbH (Germany) and Technishe Univ. München

(Germany)

11.00: Optoacoustic tomography of cancer receptors in mouse models using targeted gold nanorod conjugates (Invited Paper), Alexander A. Oraevsky, TomoWave Labs., Inc. (USA) . . . . . . . . . . . . . . . . . . . [8089-01]Three-dimensional optoacoust ic tomography system was developed for applications in preclinical cancer resarch. The applications take advantage of a targeted contrast agent based on gold nanorods conjugated with specific vectors against cancer receptors.

SESSION 2

Room: Room 5 Sun. 11.00 to 13.00

Phase and Holographic Microscopy

Session Chair: Peter T. C. So, Massachusetts Institute of

Technology (USA)

SESSION 2

Room: Room 11 Sun. 11.15 to 13.15

New Methods and Techniques for Brain

ImagingSession Chairs: Yukio

Yamada, The Univ. of Electro-Communications (Japan); Martin

Wolf, Univ. Hospital Zürich (Switzerland)

8086: Room 5Advanced Microscopy

Techniques II

8088: Room 11Diffuse Optical

Imaging III

8089: Room 2Molecular Imaging III

11.00: Quantitative phase imaging-based refractive index determination of living cells using incorporated microspheres as reference (Invited Paper), Björn Kemper, Sabine Przibilla, Angelika Vollmer, Steffi Ketelhut, Gert von Bally, Westfälische Wilhelms-Univ. Münster (Germany) . . . . . . . . . . . . . . . [8086-06]A method for the determination of the intracellular refractive index is presented. Therefore, microspheres that have been incorporated by living cells are used as reference in quantitative digital holographic phase contrast images.

11.15: Robot-assisted motor activation monitored by time-domain optical brain imaging, Oliver Steinkellner, Heidrun Wabnitz, Physikalisch-Technische Bundesanstalt (Germany); Simone Schmid, Robert Steingräber, Technische Univ. Berlin Germany); Henning Schmidt, Jörg Krüger, Fraunhofer-Institut für Produktionsanlagen und Konstruktionstechnik (Germany); Rainer Macdonald, Physikalisch-Technische Bundesanstalt (Germany) . . . [8088-06]A portable time-domain near-infrared reflectometer monitors hemodynamic brain response at four measurement posit ions. Robot-assisted upper extremity rehabilitation movements, wrist flexion-extension and forearm pronation-supination are compared with an unassisted squeeze ball exercise. Data analysis based on variance of time-of-flight distributions of photons through tissue provides a suitable representation of intracerebral signals.

11.30: Simultaneous imaging of cortical haemoglobin oxygenation and blood flow with RGB reflectometry and LASCA during stroke in rats, Andre Steimers, RheinAhrCampus Remagen (Germany); Markus Gramer, Masatoshi Takagaki, Rudolf Graf, Max-Planck-Institut für Neurologische Forschung (Germany); Matthias Kohl-Bareis, RheinAhrCampus Remagen (Germany) . . . . . . . [8088-07]We demonstrate the simultaneous mapping of haemoglobin oxygenation and blood f low changes of the exposed cortex in rats by broad-band reflectometry and laser speckle contrast analysis. A high temporal resolution was achieved by using techniques of multi- and manycore computing. As examples, spatial and temporal changes of haemoglobin oxygenation and blood flow are observed in response to middle cerebral artery occlusion and cortical spreading depression.

11.30: Multispectral optoacoustic tomography of structure and biomarkers in mice, Andreas Buehler, Daniel Razansky, Technische Univ. München (Germany); Vasilis Ntziachristos, Technische Univ. München (Germany) and Helmholtz Zentrum München GmbH (Germany) . . . . . . . . . . . . . . . [8089-02]Multispectral optoacoustic tomography (MSOT) is a recently developed imaging modality with the potential to visualize optical contrast in tissue in real-time with high spatial ultrasonic resolution. Herein we present a newly developed small animal whole body scanner and showcase its potential by visualising anatomical, dynamic and molecular information.

11.30: Dual-wavelength digital holography for quantification of cellular volume and integral refractive index, Daniel Boss, Ecole Polytechnique Fédérale de Lausanne (Switzerland); Jonas Kuehn, Ctr. Hospitalier Univ. Vaudois (Switzerland); Christian D. Depeursinge, Pierre J. Magistretti, Ecole Polytechnique Fédérale de Lausanne (Switzerland); Pierre P. Marquet, Ecole Polytechnique Fédérale de Lausanne (Switzerland) and Ctr. Hospitalier Univ. Vaudois (Switzerland) . . . . . . . . . . . . . [8086-07]We propose a Dual-wavelength Digital Holographic Microscopy (DHM) setup to measure with a single hologram a c q u i s i t i o n c e l l u l a r t h i c k n e s s and integral refractive index. The method allows to deduce important biophysical parameters of living cells including absolute volume, dry mass concentrations and water membrane permeability.

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SESSION 2 continued11.45: Holographic second harmonic phase microscopy of collagen fibers, Etienne Shaffer, Ecole Polytechnique Fédérale de Lausanne (Switzerland); Pierre Marquet M.D., Ecole Polytechnique Fédérale de Lausanne (Switzerland) and Ctr. Hospitalier Univ. Vaudois (Switzerland); Christian Depeursinge, Ecole Polytechnique Fédérale de Lausanne (Switzerland) . . . . . . . . . . . . . [8086-08]We investigate the phase of second harmonic generated by a label-free biological specimen: collagen fibers, and discuss of its interpretation. Notably, we show how the phase, qualitatively acting as an indicator of phase-matching conditions, tends to indicate that second harmonic generation, in collagen, is dominated by coherent scattering.

SESSION 2 continued11.45: Anatomical brain atlas for NIRS measurements of brain activation, Matteo Caffini, Lucia Zucchelli, Davide Contini, Rinaldo Cubeddu, Lorenzo Spinelli, Politecnico di Milano (Italy); David A. Boas, Massachusetts General Hospital (USA); Alessandro Torricelli, Politecnico di Milano (Italy) . . . . . . . . . . . . . [8088-08]Anatomical brain atlases have been introduced in the analysis NIRS data of brain activation and good spatial activation localization has been proved. We applied this method to visualize NIRS data from different protocols.

SESSION 1 continued11.45: Photoacoustic computed tomography of mice: hybrid imaging of light absorption and ultrasound transmission properties, Jithin Jose, Univ. Twente (Netherlands); Han Van Neck, Erasmus MC (Netherlands); Gerben Koning, Erasmus MC (New Zealand); Timo Ten Hagen, Erasmus MC (Netherlands); Wiendelt Steenbergen, Ton Van Leeuwen, Srirang Manohar, Univ. Twente (Netherlands) . . . . . . . . . . . . . [8089-03]We present a system which allows simultaneous imaging of optical absorption properties and acoustic transmission properties of an object in a two-dimensional slice. We make measurements of a living mouse which has tumor implanted on the lower abdomen and show that hybrid imaging is possible.

12.00: High resolution imaging with impulse based thermoacoustic tomography, Stephan Kellnberger, Amir Hajiaboli, George Sergiadis, Daniel Razansky, Vasilis Ntziachristos, Helmholtz Zentrum München GmbH (Germany) and Technische Univ. München (Germany) . . . . . . . [8089-04]Thermoacoustic tomography has potential for resolving structures deep inside tissue. One drawback is spatial resolution. We present a high resolution thermoacoustic tomography system based on excitation with ultrashort impulses. Experiments on copper wires show resolution of ~160µm. Future studies include imaging of biological tissue and small animals.


Techniques II


Imaging III


12.00: A non-contact time-domain scanning brain imaging system: results of proof of principle tests, Mikhail Mazurenka, Alexander Jelzow, Heidrun Wabnitz, Bernd Ebert, Physikalisch-Technische Bundesanstalt (Germany); Davide Contini, Politecnico di Milano (Italy); Lorenzo Spinelli, Consiglio Nazionale delle Ricerche (Italy); Antonio Pifferi, Alberto Dalla Mora, Alberto Tosi, Politecnico di Milano (Italy); Rainer Macdonald, Physikalisch-Technische Bundesanstalt (Germany) . . . . . . . . . . . . . . . [8088-09]We report proof-of-principle test results of a non-contact time-domain brain scanning system, based on recent advances: the small-distance approach and late-only photon detection with fast-gated detectors. The measured depth sensitivity and spatial resolution are compared to simulations and previous results with a fiber-based instrument.

12.15: Comparison of MRI and diffuse optical methods for calculating relative changes in the cerebral metabolic rate of oxygen, David L. Minkoff, Univ. of Pennsylvania (USA); Turgut Durduran, ICFO - Instituto de Ciencias Fotónicas (Spain); Meeri N. Kim, Dalton Hance, Erin M. Buckley, Univ. of Pennsylvania (USA); Mari Tobita M.D., Jiongjiong Wang, Joel H. Greenberg, John A. Detre M.D., The Univ. of Pennsylvania Health System (USA); Arjun G. Yodh, Univ. of Pennsylvania (USA) . . . . . . . . [8088-10]In this study, we use diffuse correlation spectroscopy (DCS) and diffuse optical spectroscopy (DOS) to estimate the cerebral metabolic rate of oxygen (CMRO2), and we compare our results to those from hypercapnia-calibrated MRI, which are acquired concurrently.

12.00: Hilbert phase microscopy combined with confocal Raman: cell image and biochemical structure, Ramachandra R. Dasari, Massachusetts Institute of Technology (USA) [8086-09]We have developed a multimodal microscopy system combining Raman micro-spectroscopy and quantitative phase microscopy and applied it to investigate blood smear samples. Three-dimensional shape of RBCs on a blood smear sample was measured and hemoglobin distribution of RBC was shown to correlate with the biconcave shape of the RBC.

12.15: Photo-acoustic imaging of blue nanoparticle targeted brain tumor for intra-operative glioma delineation, Aniruddha Ray, Univ. of Michigan (USA); Xueding Wang, Univ. of Michigan Health System (USA); Yong-Eun L. Koo, Hoe Jin Hah, Gwangseong Kim, Univ. of Michigan (USA); Thomas Chen, Daniel Orringer, Oren Sagher, Univ. of Michigan Health System (USA); Raoul Kopelman, Univ. of Michigan (USA) . . . . . . . . . [8089-05]A novel method to delineate rat brain tumors intra-operatively, as well as pre- and post-operatively, using a highly sensitive photoacoustic imaging technique enhanced by tumor targeting blue nanoparticle as contrast agent is demonstrated.

12.15: Resolution improvements by digital holographic microscopy, Yann J. Cotte, Christian Depeursinge, Ecole Polytechnique Fédérale de Lausanne (Switzerland) . . . . . . . . . . . . . [8086-10]We present a new concept for high-resolution imaging based on digital holographic microscopy. The microscope objective band pass is enlarged by a phase scanning technique. Consecutively, the novel technique of deconvolution of complex fields effectively corrects for non-ideal imaging conditions.



SESSION 2 continued12.30: Time-resolved and spectral-resolved optical imaging to study brain hemodynamics in songbirds, Stéphane Mottin, Univ. Jean Monnet Saint-Etienne (France); Bruno Montcel, Univ. Claude Bernard Lyon 1 (France); Hugues Guillet de Chatellus, Univ. Joseph Fourier (France); Stéphane Ramstein, Clémentine Vignal, Nicolas Mathevon, Univ. Jean Monnet Saint-Etienne (France) . . . . . . . . [8088-11]Time-resolved and spectral-resolved DOT with a white laser and a streak camera measured HbO2 and Hb concentration changes fol lowing p h y s i o l o g i c a l a u d i t o r y s t i m u l i in songbirds with sub-micromolar sensitivity and sub-millimeter spatial resolution.

12.45: Performance assessment of time-domain optical brain imagers, Heidrun Wabnitz, Physikalisch-Technische Bundesanstalt (Germany); Antonio Pifferi, Alessandro Torricelli, Politecnico di Milano (Italy); Mikhail Mazurenka, Oliver Steinkellner, Alexander Jelzow, Rainer Macdonald, Physikalisch-Technische Bundesanstalt (Germany); Davide Contini, Lucia Zucchelli, Lorenzo Spinelli, Politecnico di Milano (Italy); Fabrizio Martelli, Paola Di Ninni, Giovanni Zaccanti, Univ. degli Studi di Firenze (Italy); Michal Kacprzak, Piotr Sawosz, Adam Liebert, Institute of Biocybernetics and Biomedical Engineering (Poland); Rinaldo Cubeddu, Politecnico di Milano (Italy) [8088-12]The performance assessment of instruments in the “nEUROPt” project relies on (i) basic instrumental tests, (ii) the “MEDPHOT protocol” and (iii) a new protocol devoted to detection, localization and quantification of cerebral absorption changes. Homogeneous and inhomogeneous phantoms are used that are based on accurately characterized Intralipid and ink.

13.00: Comparison of temporal response to cerebral blood flow measured by laser speckle flowgraphy and laser Doppler flowmetry, Masashi Kusano, Haruka Nakayama, Keio Univ. (Japan); Hiroyuki Takuwa, Kazuto Masamoto, National Institute of Radiological Sciences (Japan); Eiji Okada, Keio Univ. (Japan); Iwao kanno, National Institute of Radiological Sciences (Japan) [8088-13]CBF change of mice during whisker stimulation is measured by laser speckle flowgraphy and laser Doppler flowmetry. Time courses of CBF measured by LSFG highly correlate with those by LDF. The results indicate that the temporal response of LSFG is sufficient to measure CBF change evoked by brain activations.

Lunch Break . . . . . . . . . 13.15 to 14.45

SESSION 2 continued12.30: Phase-coded volume holographic microscopy, Yuan Luo, Se Baek Oh, Ioannis Zervantonakis, Roger D. Kamm, George Barbastathis, Massachusetts Institute of Technology (USA) . . . . . . . . . . . . . . . . . . . [8086-11]A new imaging system incorporating phase-coded and holographic gratings is presented. In addition, we demonstrate the imaging modality to obtain spatial-spectral images of human endothelial cells in real-time.

SESSION 1 continued12.30: Plasmon resonance from novel monolayer and bilayer shell aggregate gold nanostructures, Myria Angelidou, Costas Pitris, Univ. of Cyprus (Cyprus) . . . . . . . . . . [8089-06]A novel nanostructure, the “shell aggregate,” which consists of small nanospheres aggregated around a core, is presented. The extinction efficiency of such monolayfer and bilayer shell aggregates is thoroughly investigated with appropriate simulations using the Discrete Dipole Approximation method.


Techniques II


Imaging III


12.45: High-resolution imaging of mouse anatomy with a multipurpose optoacoustic tomography system, Andreas Oancea, Daniel Razansky, Helmholtz Zentrum München GmbH (Germany); Vasilis Ntziachristos, Technische Univ. München (Germany) and Helmholtz Zentrum München GmbH (Germany) . . . . . . . . . [8089-07]A modular optoacoustic tomography system suitable for high resolution vasculature visualisation is presented. Its potential is exemplarily shown in three experiments with phantoms, mouse head vasculature and mouse tumor vasculature at various measurement conditions.

12.45: Dynamic differential image contrast by digital holography for imaging and quantitative phase microscopy, Lisa Miccio, Andrea Finizio, Istituto Nazionale di Ottica Applicata (Italy); Roberto Puglisi, Donatella Balduzzi, Andrea Galli, Istituto Sperimentale Italiano Lazzaro Spallanzani (Italy); Pietro Ferraro, Istituto Nazionale di Ottica Applicata (Italy) . . . . . . . . . . . . . . . . . . . [8086-12]Dynamic DIC by Digital Holography Microscopy for enhancing phase-contrast visualization is presented. Dynamic display of DIC phase-contrast images along all directions enhance the detection of all details in the object under investigation. Furthermore, this method allow to optimize a-posteriori the parameters for the best visualization conditions.

Lunch Break . . . . . . . . .13.00 to 14.15Lunch Break . . . . . . . . .13.00 to 14.30

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SESSION 1

Room: 3 Sun. 14.30 to 17.00

Novel Imaging Approaches

Session Chair: Alex Vitkin, Univ. of Toronto (Canada)


Techniques II


Imaging III


8090: Room 3Novel Biophotonic


SESSION 2


Novel Techniques for Molecular Visualization

Session Chair: Konstantin V. Sokolov, The Univ. of Texas M.D.

Anderson Cancer Ctr. (USA)

14.15: In vivo fluorescence sensing and enumerating of rare circulating cells with diffuse light, Mark J. Niedre, Eric W. Zettergren, Dwayne Vickers, Northeastern Univ. (USA); Judith M. Runnels, Charles P. Lin, Massachusetts General Hospital (USA) . . . . . [8089-08]In this presentation we will discuss our work in developing and validating a new instrument to sense and enumerate fluorescently labeled cells in the bloodstream mice with diffuse light. This technique will potentially allow sensing of individual cells non-invasively in vivo with sensitivity several orders of magnitude beyond current approaches.

14.30: Combined three-dimensional magnetic resonance guided optical spectroscopy and protoporphyrin IX fluorescence imaging of human breast cancer, Michael A. Mastanduno, Scott C. Davis, Shudong Jiang, Dartmouth College (USA); Roberta diFlorio-Alexander, Dartmouth Hitchcock Medical Ctr. (USA); Brian W. Pogue, Keith D. Paulsen, Dartmouth College (USA) . . . . . . . . . . . . [8089-09]We present the first 3D in-vivo images of breast cancer imaged by dynamic contrast enhanced magnetic resonance imaging, near infrared spectroscopy, and fluorescence molecular tomography in normal volunteers and cancer patients using non- invasive endogenous contrasts of hemoglobin concentration and Protoporphrin IX yield to aid in tumor quantification prior to biopsy.

14.45: Imaging small animals with a hybrid FMT-XCT system and hybrid reconstruction methods, Angelique B. F. Ale, Vladimir Ermolayev, Eva Herzog, Vasilis Ntziachristos, Helmholtz Zentrum München GmbH (Germany) and Technische Univ. München (Germany) . . . . . . . . . . . . . . . [8089-10]A hybrid FMT-XCT imaging approach was applied to small animal imaging. XCT-based anatomical information was used to improve the reconstruction of the 3d fluorescence distribution. We present imaging results for several pre-clinical mouse studies.

SESSION 3

Room: Room 11 Sun. 14.45 to 16.00

Human Head ModelsSession Chairs: Martin

Wolf, Univ. Hospital Zürich (Switzerland); Hamid Dehghani, The Univ. of Birmingham (United

Kingdom)

SESSION 3


Molecular Imaging and Novel

Geometries ISession Chair: Irene

Georgakoudi, Tufts Univ. (USA)

14.30: An endoscopic structured lighting probe using spectral encoding, Neil T. Clancy, Danail Stoyanov, Guang-Zhong Yang, Daniel S. Elson, Imperial College London (United Kingdom) . . . . . [8090-01]A simple fibre probe is reported that encodes a structured light pattern spectrally to recover the 3D surface shape of tissue in minimally invasive surgery. This enables robust identification of specific projected features.

14.30: Towards in vivo tomographic FLIM-FRET imaging of biomolecular interactions (Invited Paper), James A. McGinty, Harriet B. Taylor, Daniel W. Stuckey, Imperial College London (United Kingdom); Vadim Y. Soloviev, Univ. College London (United Kingdom); Romain Laine, Lingling Chen, Laurence Bugeon, Olivier E. Pardo, Jonathan R. Lamb, Alessandro Sardini, Imperial College London (United Kingdom); Simon R. Arridge, Univ. College London (United Kingdom); Jo V. Hajnal, Margaret J. Dallman, Paul M. W. French, Imperial College London (United Kingdom) . . . . . . . . . . [8086-13]We present two techniques for 3-D fluorescence lifetime imaging; one applied to small (~mm) transparent samples (e.g. zebrafish) and the other to large (~cm) heavily scattering samples (e.g. mice). Both rely on time-gated fluorescence acquisition with rotational scanning of the sample followed by appropriate the reconstruction procedure. We have applied these techniques to imaging non-cleared transgenic zebrafish embryos and electroporated FRET constructs in mice.

14.45: Comparative study of algorithms to derive changes in hemoglobin concentrations from time domain near infrared spectroscopy measurements, Norbert S. Zolek, Adam Liebert, Daniel Milej, Michal Kacprzak, Institute of Biocybernetics and Biomedical Engineering (Poland); Wojciech Weigl, Medical Univ. of Warsaw (Poland); Alessandro Torricelli, Lorenzo Spinelli, Davide Contini, Matteo Caffini, Lucia Zucchelli, Rinaldo Cubeddu, Politecnico di Milano (Italy); Alexander Jelzov, Oliver Steinkellner, Heidrun Wabnitz, Physikalisch-Technische Bundesanstalt (Germany); Stefan P. Koch, Jens M. Steinbrink, Charité Universitätsmedizin Berlin (Germany) . . . . . . . . . . .[8088-14]Different algorithms for analysis of time-resolved reflectance signals, based on moments and time windows, were compared. They were applied to data from a motor-stimulation study on healthy subjects performed by the various institutions according to a common measurement protocol. The comparison is related to the assessment

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14.45: Assessment of flow rates in holographic laser Doppler imaging, Caroline V. Magnain, Michael Atlan, Ecole Supérieure de Physique et de Chimie Industrielles (France) [8090-02]We report velocity assessment methods in holographic laser Doppler imaging in-vitro and in-vivo. Our strategy relies either on the calculation of the two first moments of Doppler distributions or on the fitting of analytical spectra derived from the Diffusing-Wave Spectroscopy formalism.



SESSION 3 continued15.00: Experimental estimation of the sensitivity profile of time-resolved reflectance measurement in a two-layered physical phantom mimicking human head, Piotr Sawosz, Michal Kacprzak, Institute of Biocybernetics and Biomedical Engineering (Poland); Wojciech Weigl, Medical Univ. of Warsaw (Poland); Norbert S. Zolek, Stanislaw Wojtkiewicz, Roman Maniewski, Adam Liebert, Institute of Biocybernetics and Biomedical Engineering (Poland) [8088-15]The time-gated ICCD camera was applied for time-resolved imaging of light penetrating in an optically turbid medium in the plane perpendicular to the axes of source and detector. Spatial distribution of light penetration probability was determined.

SESSION 3 continued15.00: Super-resolution stochastic fluctuation microscopy: a comparison between SOFI and STORM, Stefan Geissbühler, Claudio Dellagiacoma, Theo Lasser, Ecole Polytechnique Fédérale de Lausanne (Switzerland) . . . . . . . . . . . . . [8086-14]STORM and SOFI are two super-resolution microscopy techniques relying on stochastically blinking emitters. Our face-to-face comparison reveals that STORM can deliver higher resolution enhancements but imposes significant constraints on the blinking behavior of the probes, while SOFI works over a wide range of photo-switching kinetics and with lower photon yields.

SESSION 2 continued15.00: Detection and visualization improvement of Spermatozoa cells by digital holography, Lisa Miccio, Andrea Finizio, Pasquale Memmolo, Melania Paturzo, Francesco Merola, Istituto Nazionale di Ottica Applicata (Italy); Giuseppe Coppola, Giuseppe Di Caprio, Mariano Gioffrè, Istituto per la Microelettronica e Microsistemi (Italy); Donatella Balduzzi, Roberto Puglisi, Andrea Galli, Istituto Sperimentale Italiano Lazzaro Spallanzani (Italy); Pietro Ferraro, Istituto Nazionale di Ottica Applicata (Italy) . . . . . . [8089-11]Numerical analysis is implemented to investigate biological sample from Digital Holographic recording. The aim is to improve visualization and detection of spermatozoa. Digital recorded holograms are numerically manipulated to retrieve, besides usual Quantitative Phase Map, DIC imaging. Furthermore, digital self-referencing approach is described, useful for specimen flowing in microfluidic channels.15.15: 3D imaging of tumor cell spheroids, Verena Richter, Thomas Bruns, Petra Weber, Hochschule Aalen (Germany); Rainer Wittig, Wolfgang S. L. Strauss, Univ. Ulm (Germany); Herbert Schneckenburger, Hochschule Aalen (Germany) . . . . . . . . . . [8089-12]A novel setup for fluorescence imaging of 3-dimensional cell cultures is described. The method is based on structured illumination by various light sources and detection of single images or reaction kinetics in individual cellular planes.

SESSION 3

Room: 2 Sun. 15.30 to 17.45

Optical and Optoacoustic Probes

and LabelingSession Chair: Alexander A.

Oraevsky, TomoWave Labs., Inc. (USA)

15.30: Receptor mediated aggregation of plasmonic nanoparticles in optical and photoacoustic molecular imaging of cancer (Invited Paper), Konstantin V. Sokolov, The Univ. of Texas M.D. Anderson Cancer Ctr. (USA); Jesse S. Aaron, Srivalleesha Mallidi, Stanislav Y. Emelianov, The Univ. of Texas at Austin (USA) . . . . . . . . . . . . . . . . . . . [8089-13]Targeted gold nanoparticles undergo dramat ic opt ica l changes upon binding to activated EGF receptors providing a sensitive way for detection of highly proliferative tumor cells. We demonstrated this concept in multi-wavelength photoacoustic imaging of 3D distribution of viable EGFR-expressing cancer cells in-vivo.

SESSION 1 continued15.00: Cell death detection and ionic homeostasis monitoring with digital holographic microscopy, Nicolas Pavillon, Jonas Kühn, Pascal Jourdain, Christian D. Depeursinge, Pierre J. Magistretti, Pierre P. Marquet, Ecole Polytechnique Fédérale de Lausanne (Switzerland) . . . . . . . . . . . . . [8090-03]We demonstrate the capability of digital holography to detect non-invasively cell death through the measurement o f ce l l u l a r vo lume regu la t i on , considered as an early indicator of homeostasis deregulation, leading to cell death triggering. We correlate those measurements with calcium imaging in neuronal cells, as a link to ionic homeostasis.


Techniques II


Imaging III




15.15: Holographic microscopy for the three-dimensional exploration of light scattering from gold nanomarkers in biological media, Fadwa Joud, Frédéric Verpillat, Lab. Kastler Brossel (France); Michael Atlan, Ecole Supérieure de Physique et de Chimie Industrielles (France); Marie Abboud, Saint Joseph Univ. of Beirut (Lebanon); Michel Gross, Lab. Kastler Brossel (France) . . . . . . . . . . [8090-04]3D structure of l ight scatter ing from live 3T3 cells labelled with 40 nm gold nanomarkers is explored using holographic microscopy. The nanoparticles scattered fields, contrarily to the cellular structures, gives straight bright scattering patterns located along the optical axis. This characterization is an unambiguous signature of the gold biological nanomarkers

15.15: Non-invasive determination of the optical properties of the human head using a neural network, Marion Jäger, Alwin Kienle, Univ. Ulm (Germany) . . . . . . . . . . . . . . . [8088-16]Light propagation in an N-layered model of the human head is investigated using Monte Carlo simulations and solutions of the diffusion theory. For the inverse problem - the determination of the optical properties of brain or scalp and skull from simulated reflectance curves - a neural network is applied.

15.15: 3D laser-scanning intersecting plane tomography (L-SIPT) for high speed, translationless volumetric microscopy, Matthew B. Bouchard, Elizabeth M. C. Hillman, Columbia Univ. (USA) . . . . . . . . . . . . . . . . . . . [8086-29]We describe a new optical imaging geometry for high speed, translationless volumetric optical imaging called Laser-Scanning Intersecting Plane Tomography (L-SIPT). This geometry can image fluorescence contrast in minimally to moderately scattering samples ranging in size from micro- to macroscale. The system design and proof of principle results are presented.

15.30: Fourier phase contrast multimodal optical microscopy for real time display of phase and fluorescence at the same time, Chandra S. Yelleswarapu, Bhargab Das, Alexey Veraksa, Devulapalli V. G. L. N. Rao, Univ. of Massachusetts Boston (USA). . . . . . . . . . . . . [8090-05]Novel Fourier phase contrast multimodal microscopy technique is demonstrated for simultaneous recording of phase and fluorescence features of live cells in real time without need for digital fusion. One can record the sequence of events in a movie. Spatial frequency filtering enables display of shape and size of live cells.

15.30: Three-dimensional superposition of diffuse optical tomography results and subjacent anatomic structures, Christina Habermehl, Christoph H. Schmitz, Charité Universitätsmedizin Berlin (Germany); Jan Mehnert, Susanne Holtze, Max-Planck-Institut für Kognitions- und Neurowissenschaften (Germany); Jens M. Steinbrink, Charité Universitätsmedizin Berlin (Germany) . . . . . . . . . . . . . . . [8088-17]We demonstrate two methods to co-register three-dimensional activation volumes obtained from high density optical tomography of human brain function (1) with the individual’s MR- scan and (2) with a generic brain model.

15.30: The tissue culture light sheet-based fluorescence microscope (TC-LSFM) paves the way to physiological assays based on three-dimensional cell cultures, Francesco Pampaloni, Johann Wolfgang Goethe-Univ. Frankfurt am Main (Germany) . . . . . . . . . . . . . . . [8086-16]Conventional two-dimensional (2D) cell monolayers do not reproduce the complex three-dimensional architecture of tissues. Thus, 2D cellular assays have a limited physiological significance. Assays based on 2D cell cultures cannot predict the response of real tissues to toxic compounds or drugs. In contrast, three-dimensional (3D) cell cultures significantly improve the physiological relevance of cellular assays and provide e.g. a valid animal-free alternative for the testing of chemicals and drugs. We present the TC-LSFM, a 3D cell culture imaging workstation based on light sheet-based fluorescence microscope (LSFM). We present studies performed on tumor cellular spheroids, on stem cell spheroids, on kidney epithelium, and on mouse dermis explant. We demonstrate the potential of TC-LSFM for high-content screening analysis.

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SESSION 3 continued15.45: Optimized implementation of focal modulation microscopy, Shau Poh Chong, Guangjun Gao, Nanguang Chen, National Univ. of Singapore (Singapore) . . . . . . . . . . . . . . [8086-17]Focal modulation microscopy (FMM) is an emerging method for high-resolution imaging of thick biological tissues. It has demonstrated a penetration depth significantly greater than that of confocal microscopy (CM). FMM is compatible with both fluorescence and scattering/reflection contrast mechanisms and can provide multi-contrast images simultaneously. A spatial-temporal phase modulator (SPM) is the critical component in FMM, which generates intensity modulation within the focal volume. The configuration and implementation of the SPM have profound impacts on the performances of the FMM system. To achieve real-time or near-real-time image acquisition, a high modulation frequency (~MHz) is necessary. In the same time, FMM signal strength depends on the aperture segmentat ion of the modulator. According to our theoretical study, a four-zone or six-zone annular aperture leads to a modulation depth of 0.72 or 0.82, respectively, which is very close to the ideal value of 1.

Coffee Break . . . . . . . . .16.00 to 16.30

SESSION 4


Nonlinear Microscopy: SHG, THG, Multimodal

Session Chair: Emmanuel Beaurepaire, Ecole


SESSION 3 continued15.45: Segmentation of magnetic resonance images to construct human head model for diffuse optical imaging, Kazuki Kurihara, Yosuke Takahashi, Keio Univ. (Japan); Hiroshi Kawaguchi, Takayuki Obata, National Institute of Radiological Sciences (Japan); Eiji Okada, Keio Univ. (Japan) . . . . . . . . . . . . . . . . . . [8088-18]We proposed a tool to segment MR head images to construct accurate human head model for diffuse optical imaging. The results obtained by the proposed tool is validated by the results by manual analysis. The results show the proposed tool can accurately extract the scalp, skull and CSF regions.

SESSION 3 continued SESSION 1 continued15.45: In vivo characterization of tumor and tumor vascular network using a multi-mode imaging approach, Vyacheslav Kalchenko, Yuri Kuznetsov, Weizmann Institute of Science (Israel); Igor Meglinski, Univ. of Otago (New Zealand); Alon Harmelin, Weizmann Institute of Science (Israel) . . . . . [8090-06]We present a dual-mode imaging approach utilizing a combined use of Fluorescence Intravital Microscope and Dynam-ic Light Scattering for in vivo characterization and visualization of tumor and tumor vascular network in the dorsal skin fold window chamber in mouse. The application of Fluorescence Intravital Microscope and Dynamic Light Scattering allows to combine the advantages of both techniques while improving on their respective limitations. Fluorescence Intravital Microscope has been used for tumor visualization, whereas Dynamic Light Scattering applied for imaging of blood vessels with the higher spatial resolution. The results demonstrate that such a dual-mode combined application of Fluorescence Intravital Microscope and Dynamic Light Scattering has a great potential and can significantly expand the capabilities of tumor angiogenesis studies and notably contribute to the development of cancer treatment.

Coffee Break . . . . . . . . .16.00 to 16.30


Techniques II


Imaging III




Coffee Break . . . . . . . . .16.00 to 16.30

SESSION 4

Room: Room 11 Sun. 16.30 to 18.00

Modeling of Light Propagation

Session Chairs: Hamid Dehghani, The Univ. of Birmingham (United

Kingdom); Andreas H. Hielscher, Columbia Univ. (USA)

16.30: Simulating light propagation: towards realistic tissue models, Hidayet G. Akarçay, Jaroslav Rička, Univ. Bern (Switzerland) .[8088-19]We present here a tool for Monte Carlo simulations of polarized light transport in three-dimensional biological tissue samples (generated from a user-created catalogue of materials and scattering laws) using relatively complex geometries. This tool can be adapted to a wide range of different scenarios thanks to a user interface.

Coffee Break . . . . . . . . .16.00 to 16.30

16.30: Specific activation of photodynamic molecular beacons: an image-guided therapeutic approach for vertebral metastases, Tracy W. B. Liu, Juan Chen, Ontario Cancer Institute (Canada); Margarete K. Akens, Lisa Wise-Milestone, Sunnybrook Health Science Ctr. (Canada); Brian C. Wilson, Gang Zheng, Ontario Cancer Institute (Canada) . . . . . . . . . . . . . . . . . .[8089-14]Breast cancer is the second leading cause of cancer-related death in women with 80% of advanced cases developing spinal metastases. We demonstrate the metastatic-specific activation of photodynamic molecular beacons as a useful image-guidance tool with an unprecedented level of PDT selectivity for the therapeutic management of spinal metastases.

16.30: Optical recording of action potential propagation in t-tubular network (Invited Paper), Leonardo Sacconi, Raffaele Coppini, Cecilia Ferrantini, Jacopo Lotti, Chiara Tesi, Elisabetta Cerbai, Univ. degli Studi di Firenze (Italy); Ping Yan, Leslie M. Loew, Univ. of Connecticut Health Ctr. (USA); Corrado Poggesi, Francesco S. Pavone, Univ. degli Studi di Firenze (Italy) . . . . . . . . . . . . . . . . . . . [8086-18]We developed an ultrafast random access two-photon microscope that, in combination with a novel voltage sensitive dye, was capable of optically recording action potential propagation in multiple positions of the T-tubular network. Our measurements show a tight electrical coupling in the entire T-tubular network.

16.30: Intravascular laser speckle imaging (ILSI): in vivo evaluation of the mechanical properties of coronary plaques in living swine, Seemantini K. Nadkarni, Jimmy D. Toussaint, Zeinab Hajjarian, Harvard Medical School (USA) . . . . . . [8090-07]Acute myocardial infarction is frequently preceded by the rupture of mechanically compromised coronary plaques. In this study, we have fabricated an intracoronary catheter and console and have demonstrated the first in vivo studies in living swine to enable the mechanical characterization of unstable plaques using Intravascular Laser Speckle Imaging (ILSI).

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SESSION 4 continued16.45: Analytical solutions of the radiative transfer equation for the fluence and radiance in infinite turbid media, André Liemert, Alwin Kienle, Univ. Ulm (Germany) . . . . . . . [8088-20]Fast and simple analytical expressions for the f luence and radiance of the steady-state radiative transfer equation in an infinitely extended and anisotropically scattering medium were derived for different source types.

SESSION 4 continued17.00: Multiphoton imaging of the cornea: polarization-resolved second harmonic generation from stromal collagen lamellae, Gaël Latour, Ivan Gusachenko, Ecole Polytechnique (France); Laura Kowalczuk, Karsten Plamann, Ecole Nationale Supérieure de Techniques Avancées (France); Emmanuel Beaurepaire, Marie-Claire Schanne-Klein, Ecole Polytechnique (France) . . . . . . . . . . . . . . . . . [8086-19]We developed a polarization-resolved multiphoton microscope to study the forward- and backward- second harmonic generation signals from corneal collagen. The orientation of the collagen lamellae is retrieved using an image processing based on a tensorial analysis of the non-linear response of the tissue.

17.15: Multimodal nonlinear optical microscopy used to discriminate epithelial ovarian cancer, Javier F. Adur, Vitor B. Pelegati, Andre A. de Thomaz, Diogo B. Almeida, Fatima Bottcher-Luiz, Liliana Andrade, Carlos Lenz Cesar, Univ. Estadual de Campinas (Brazil) . . . . . . . . . [8086-20]In this work, we tested the feasibility of nonlinear imaging approaches, the two-photon excitation fluorescence, second harmonic generation, and third-harmonic generation to serve as complementary tools for ovarian cancer diagnosis. These nonlinear optical techniques allow discriminating between normal and neoplastic tissues.

SESSION 1 continued16.45: Advanced multifunctional imaging and sensing techniques based on confocal microscopy and optical coherence tomography, Ilko K. Ilev, U.S. Food and Drug Administration (USA) . . . . . . . . . . . . . . . . . . . [8090-08]We demonstrate some advanced biophotonic multifunctional imaging and sensing techniques based on simple fiber-optic confocal microscopy and optical coherence tomography approaches. These noninvas ive techniques have been exploited for high resolution combined bioimaging and biosensing applications in the areas of ophthalmology and optical nerve stimulation.

SESSION 2


Emerging Technologies: Models and Platforms ISession Chair: Seemantini K. Nadkarni, Harvard Medical

School (USA)

17.00: Development of photonic crystal fiber-based SERS platform for biosensing applications, Dinish U. K. Soudamini Amma, Chit Yaw Fu, Kiat Seng Soh, Singapore Bioimaging Consortium (Singapore); Ramaswamy Bhuvaneswari, National Cancer Ctr. of Singapore (Singapore); Malini C. Olivo, Singapore Bioimaging Consortium (Singapore) . . . . . . . . . . . . . . [8090-09]

SESSION 3 continued16.45: Cryoslicing-imaging: a method for multispectral imaging of fluorescent agents bio-distribution in small animals, Athanasios Sarantopoulos, George Themelis, Helmholtz Zentrum München GmbH (Germany); Vasilis Ntziachristos, Helmholtz Zentrum München GmbH (Germany) and Technishe Univ. München (Germany) . . . . . . . . .[8089-15]We repor t the deve lopment o f a multispectral imaging system that captures high-resolution three dimensional color and fluorescence volumes of optical contrast agents in small animals. Our approach can be used both as an imaging method to detect fluorescence bio-distribution and as a validation method for tomographic modalities.

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Imaging III




17.00: Cardiac safety screens: molecular, cellular and optical advancements, Lars Kaestner, Qinghai Tian, Peter Lipp, Univ. des Saarlandes (Germany) . . . . . . . . . . . . . . . . .[8089-16]Optical methods can be used for QT-interval investigations based on cellular action potentials using either the small molecule dye di-8-ANEPPS or the genetically encoded sensor Mermaid. Adult cardiomyocytes are superior to neonatal cardiomyocytes for such pharmacological investigations. Optical QT-screens may replace intricate animal experiments.

17.00: Fluorescence light transfer in biological tissues by coupled time-dependent parabolic simplified spherical harmonics equations, Jorge Bouza-Domínguez, Yves Bérubé-Lauzière, Univ. de Sherbrooke (Canada) . . . . . . . . . . . . . . . . [8088-21]Coupled parabolic simplified spherical harmonics equations are derived for the propagation of excitation and fluorescence light in biological tissues in the time domain. These are numerically solved using finite elements/differences for the spatial/temporal dependencies. Results are given for the propagation in a small animal with an organ filled with a fluorescent probe.

17.15: A finite-volume algorithm for modeling light transport with the simplified spherical harmonics approximation, Ludguier D. Montejo, Hyun-Keol Kim, Andreas H. Hielscher, Columbia Univ. (USA) . . . . . . [8088-22]A c e l l - c e n t e r e d f i n i t e v o l u m e approximation to the simplified spherical harmonics (SPN) equations for modeling light propagation in tissue is introduced. The resulting system of algebraic equations is solved with the generalized minimal residual (GMRES) algorithm.

17.15: Multifunctional rare-earth vanadate nanoparticles: fluorescent probes, hydrogen peroxide sensors and MRI contrast agents, Markus D. Schoeffel, Cédric Bouzigues, Thierry Gacoin, Jean-Pierre Boilot, Ecole Polytechnique (France); Olivier Clément, INSERM (France); Antigoni Alexandrou, Ecole Polytechnique (France) .[8089-17]Pho tos tab le and non b l i nk ing Gd0.6Eu0.4VO4 rare earth vanadate nanoparticles show red fluorescence due to Eu3+ emission. These nanoparticles can be photoreduced and thus become sensitive to the surrounding oxidant concentration. We have demonstrated detection of hydrogen peroxide based on emission intensity modulation. In addition, these nanoparticles show a relaxation enhancing effect on protons and can be used as positive MRI contrast agents. Thus, Gd0.6Eu0.4VO4 nanoparticles are attractive multifunctional probes.

17.15: Lung cancer targeted Raman active phospholipid gold nanoparticles for ultrasensitive and specific molecular imaging and detection, Natalie Tam, Benjamin Scott, Brian C. Wilson, Gang Zheng, Ontario Cancer Institute (Canada) . . . . . . . . . . . . . . . . [8090-10]For improving specificity of lung carcinoma detection, a novel surface enhanced Raman spectroscopy nanoparticle that encapsulates dye molecules on colloidal gold coated with a phospholipid shell conjugated to an antibody/peptide was designed and used. This platform combines the molecular specificity of the targeting ligand with the ultrasensitivity of SERS detection.



SESSION 4 continued17.30: Imaging amylopectin’s order in starch using 3-dimensional polarization SHG, Sotiris Psilodimitrakopoulos, Ivan Amat-Roldán, David Artigas-García, Pablo Loza-Alvarez, ICFO - Instituto de Ciencias Fotónicas (Spain) . . [8086-21]We perform polarization SHG (PSHG) imaging, using a generalized 3D biophysical model, to quantify the degree of organization of amylopectin molecules in starch. To do so, the 3D-PSHG technique is used in dry and hydrated starch granules in which amylopectin molecules are less and more ordered respectively.

SESSION 4 continued17.30: Importance of considering exact boundary conditions of the probe geometry on determination of the optical properties of turbid media, Oliver Fugger, Alwin Kienle, Univ. Ulm (Germany) . . . . . . . [8088-23]Light propagation in semi-infinite turbid media is studied in the steady-state domain by taking into account exact boundary conditions at the probe-medium interface.

SESSION 2 continued17.30: Development of Au/Ag substrate with alternating nanosphere array for SERS-based biosensing, Chit Yaw Fu, Dinish U. K. Soudamini Amma, Singapore Bioimaging Consortium (Singapore); Kiang-Wei Kho, National Cancer Ctr. of Singapore (Singapore); Wenda Goh, Singapore Bioimaging Consortium (Singapore); Malini C. Olivo, National Univ. of Ireland, Galway (Ireland) . . . . . . . . . . . . . . . . . [8090-11]Au/Ag SERS-substrate of optimal Au/Ag thickness ratio was shown to render SERS enhancement and stability exceeding those of the Au-coated substrate. Nanosphere array of alternating sizes was also constructed to increase the number of contact points between the nanospheres that could possibly lead to greater SERS enhancement.

17.45: Investigation of protein aggregation dynamics with a Bloch surface wave sensor, Vincent Paeder, Sara Santi, Valeria Musi, Hans Peter Herzig, Ecole Polytechnique Fédérale de Lausanne (Switzerland) . . [8090-12]We present a study of the dynamics of protein aggregation using a common path heterodyne Bloch surface wave sensing scheme. We demonstrate the ability to detect, during thermal incubation, the early events linked to the aggregation of proteins related to conformational diseases. Hen Egg White Lysozyme and Alzheimer’s amyloid-β 1-42 are used as model systems to demonstrate the efficiency of the method.

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SESSION 3 continued17.30: Nonlinear optical molecular imaging enables metabolic redox sensing in tissue engineered constructs, Leng-Chun Chen, William R. Lloyd III, Robert H. Wilson, Shiuhyang Kuo, Cynthia L. Marcelo, Stephen E. Feinberg, Mary-Ann Mycek, Univ. of Michigan (USA) . . . . . . . . . . . . . . . . . . . [8089-18]Tissue-engineered constructs require noninvasive monitoring of cellular viability before implantation. In a preclinical study, nonlinear optical molecular imaging was employed to extract morphological and functional information from intact constructs. Nonlinear optical molecular imaging has the potential to visualize and quantitatively monitor growth and viability of tissue-engineered constructs over time.

Conference End.17.45: Spectral reflectance fitting based on Monte Carlo simulation using a multi-layered skin tissue model, Yoshihisa Aizu, Muroran Institute of Technology (Japan); Takaaki Maeda, Kushiro National College of Technology (Japan); Tomohiro Kuwahara, Tetsuji Hirao, Shiseido Co., Ltd. (Japan) . . . . . . . . . . . . . . [8088-24]Nine-layered skin tissue model is developed for Monte Carlo simulation of spectral reflectance. By finding appropriate optical and geometrical parameters, a simulated spectrum can be produced to agree well with a measured spectrum.

17.45: Multimodal multiscale three-dimensional optical imaging of rodent brain slices, Sabine Donner, Alexander Krüger, Laser Zentrum Hannover e.V. (Germany); Kerstin Schwabe, Medizinische Hochschule Hannover (Germany); Anaclet Ngezahayo, Leibniz Univ. Hannover (Germany); Alexander Heisterkamp, Laser Zentrum Hannover e.V. (Germany) . . . . . . . . . . . . . . . [8086-22]Accurate placement of the electrode is crucial for deep brain stimulation and could be achieved through intra-operative microscopy. Optical coherence tomography, two photon laser scanning microscopy and confocal laser scanning microscopy in reflective mode are tested in a combined setup on brain slices from rodents.



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Mon. 09.30 to 11.00

World of Photonics Opening Plenary


Techniques II


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SESSION 5

Room: 5 Mon. 11.15 to 12.45

Nonlinear Microscopy: CARS, FWM, Pulse

Shaping, TechnologySession Chair: Peter T. C. So,

Massachusetts Institute of Technology (USA)

11.15: Accessing nonlinear phase contrast in biological tissue using femtosecond laser pulse shaping (Invited Paper), Martin C. Fischer, Prathyush Samineni, Baolei Li, Kevin Claytor, Warren S. Warren, Sr., Duke Univ. (USA) . . . . . . . . . .[8086-23]We demonstrate nonlinear optical phase contrast imaging in highly scattering media using rapid femtosecond laser pulse shaping. We will also discuss potential applications of this technique for intrinsic functional neuronal imaging.

SESSION 5

Room: 11 Mon. 11.15 to 12.45

Optical Tomography ISession Chairs: Andreas H.

Hielscher, Columbia Univ. (USA); Subhadra Srinivasan, Dartmouth

College (USA)

SESSION 3

Room: B13 Mon. 11.15 to 12.30

Emerging Technologies: Models and Platforms IISession Chair: Arjen Amelink,

Erasmus MC (Netherlands)

11.15: Compressive diffuse optical tomography (Invited Paper), Mehmet Suzen, Alexia Giannoula, Turgut Durduran, ICFO - Instituto de Ciencias Fotónicas (Spain) .[8088-25]Diffuse optical tomography (DOT) is the 3D, non-invasive, reconstruction of the tissue optical properties for biomedical applications. DOT suffers from severe under-sampling which leads to image artifacts requiring a large number of measurements to minimize these artifacts. We have recently introduced a compressed sensing (CS) framework for DOT which uses a sparsifying basis, l1-regularization and random sampling to reduce the number of measurements that are needed to achieve a certain accuracy. In fact, it allows for sub-Shannon-Nyquist limit sampling of the signals when certain conditions are met. We introduce CS for DOT, demonstrate the utility its utility using numerical simulations and discuss the latest progress of CS for DOT. Overall, the results show improvements in comparison to “traditional” linear reconstruction methods. Furthermore, CS is shown to be more robust against the reduction of measurements.

11.15: Polymer waveguide platform for highly integrated biophotonics, Hamid Keshmiri, Bjorn Agnarsson, Kristjan Leosson, Univ. of Iceland (Iceland) . . . . . . . . . . . . . . . . . [8090-13]A novel, highly integrated, optical platform for biophotonics is presented, featuring a cladding index that closely matches aqueous samples or live cells. Applications including live-cell evanescent-wave microscopy and on-chip manipulation of light signals are demonstrated.

11.30: Mesh-based Monte Carlo code for fluorescence modeling in complex tissues with irregular boundaries, Robert H. Wilson, Leng-Chun Chen, William R. Lloyd, Shiuhyang Kuo, Cynthia L. Marcelo, Stephen E. Feinberg, Mary-Ann Mycek, Univ. of Michigan (USA) . . . . [8090-14]We describe the development and validation of a user-friendly, MATLAB-based Monte Carlo code that uses analytically-defined surface meshes to model tissue geometry. The code can be employed to discriminate the detected fluorescence from different layers of complex turbid media for which the interfaces between the layers are irregular in shape.

11.45: Characterization of nanoparticles using yhird harmonic generation correlation spectroscopy, Matthias Geissbuehler, Iwan Märki, Noelia Bocchio, Ecole Polytechnique Fédérale de Lausanne (Switzerland); Vladislav I. Shcheslavskiy, Becker & Hickl GmbH (Germany); Theo Lasser, Ecole Polytechnique Fédérale de Lausanne (Switzerland) . . . . . [8090-15]Nanoparticles are interesting for biomedical imaging as novel non-fluorescent non-bleaching labels. We present the characterization of freely diffusing nanoparticles using third harmonic generation correlation spectroscopy by illumination with a focussed pulsed femtosecond laser. We present the optical system as well as first spectroscopic results on nanoparticles.

11.45: Optical projection tomography for light scattering media, Vadim Y. Soloviev, Simon R. Arridge, Univ. College London (United Kingdom) . . . . . . . . . . . . . . . [8088-26]We consider the problem of optical tomographic imaging in a weakly scattering medium with presence of highly scattering inclusions. The approach is based on the assumption that the transport coefficient differ by an order of magnitude in weakly and highly scattering regions. The approach was verified by reconstructions of optical parameters from numerically simulated datasets.

11.45: Time-multiplexed two-photon microscopy using broadband shaped femtosecond pulses, Guillaume Labroille, Rajesh Sreedharan, Xavier Solinas, Ecole Polytechnique (France); Caroline Boudoux, Ecole Polytechnique de Montréal (France); Nicolas Olivier, Emmanuel Beaurepaire, Manuel Joffre, Ecole Polytechnique (France) .[8086-24]We demonstrate two methods for achieving multiplexed two-photon imaging in vivo with fast pixel rates and micrometer resolution. Using coherent control of the two-photon excited fluorescence, we performed selective microscopy in developing Drosophila embryos.



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SESSION 3 continued12.00: Fabrication and characterization of real-time optical fiber dosimeter probe, Andre Croteau, Serge Caron, INO (Canada); Alexandra Rink, David A. Jaffray, Princess Margaret Hospital (Canada); Ozzy Mermut, INO (Canada) . . . . . [8090-16]There is a pressing need for a low cost and passive optical fiber dosimeter probe for use in real-time monitoring and quality control of x-rays dose delivered in clinical radiotherapy. Our optical probe solution consists of a radiochromic material sandwich between one end of an optical fiber and a mirror.

SESSION 5 continued12.00: Diffuse optical tomography of absorption in biological media using time-dependent parabolic simplified spherical harmonics equations, Jorge Bouza-Domínguez, Yves Bérubé-Lauzière, Univ. de Sherbrooke (Canada) . . . . . . . . . . . . . . . [8088-27]We present a diffuse optical tomography algorithm for absorption imaging in biological tissues using time-domain measurements. It uses time-dependent parabolic simplified spherical harmonics equations as the forward model and a nested analysis and design method developed for solving constrained optimization problems. Results for geometries mimicking small animal imaging are given.

SESSION 5 continued12.00: Non linear optical microscopy of adipose-derived stem cells induced towards osteoblasts and adipocytes, Rabah Mouras, Pierre O. Bagnaninchi, Andrew R. Downes, Massimo Muratore, Alistair P. D. Elfick, The Univ. of Edinburgh (United Kingdom) . . . . . . . . . . . . . . . . .[8086-25]Adipose-derived stem cells (ADSC) are a realistic autologous source for cell-based orthopaedic and reconstructive therapies. Label-free, non-invasive and quantitative methods are required to assess the effectiveness of stem cell (SC) differentiation and to ensure efficient translation of SC technologies to clinical practice. We report on the development and application of a multimodal microscope to monitor and quantify ADSC differentiation into osteoblast and adipocyte phenotypes.

12.15: New biomedical opportunities offered by compact sub-nanosecond supercontinuum sources, Philippe Leproux, Vincent Couderc, Univ. de Limoges (France); Guillaume Huss, Leukos (France) . . . . . . . . . . . .[8086-26]This talk will review recent developments and biomedical applications of compact supercontinuum laser sources, based on the combination of an infrared sub-nanosecond microchip laser (pulse duration < 1 ns) and a photonic crystal fibre. These results relate to optical coherence tomography (OCT), coherent anti-Stokes Raman scattering (CARS) microspectroscopy and flow cytometry.


Techniques II


Imaging III



12.15: A semi-analytical perturbation model for diffusion tomogram reconstruction from time-resolved optical projections, Alexander B. Konovalov, Vitaly V. Vlasov, Alexander S. Uglov, Russian Federal Nuclear Ctr. - All-Russian Research Institute of Experimental Physics (Russian Federation); Vladimir V. Lyubimov, S.I. Vavilov State Optical Institute (Russian Federation) . . . . . . . . . . . . . . [8088-28]This paper proposes a perturbation model for time-domain diffuse optical tomography in the flat layer transmission geometry. To evaluate the imaging operator, the Green’s function of the diffusion equation for a semi-infinite scattering medium with the Robin boundary condition is used.

12.30: A semi-analytic method for continuous-wave diffuse optical tomography, Ravi Prasad K. Jagannath, Phaneendra K. Yalavarthy, Indian Institute of Science (India) . . . . . . . . . . . . . . . . . . . [8088-29]We propose a semi-analytic method for DOT, that uses modified Beer’s Law for updating the Jacobian along with calculation of forward data. It is shown that the absorption coefficient distributions match with the traditional reconstruction results within 1% and the computational speed-up is up to a factor of seven.

12.15: Comparison of Monte Carlo simulations of polarized light propagation in turbid media with exact Maxwell solutions, Ansgar Hohmann, Florian Voit, Jan Schäfer, Alwin Kienle, Univ. Ulm (Germany) . . . . . . . . . . . . . . . [8090-17]A Monte Carlo program for simulation of polarized light propagation in scattering media was developed. The results were compared to the results of the exact Maxwell solution. This approach facilitates verification of polarized light Monte Carlo programs.

Lunch and poster viewing . . . . . . . . . . . . .12.30 to 14.00

12.30: Automatic laser alignment for multifocal microscopy using a LCOS SLM and a 32×32 pixel CMOS SPAD array, David Tyndall, Richard Walker, The Univ. of Edinburgh (United Kingdom); Krzysztof Nguyen, The Univ. of Edinburgh Group (United Kingdom); Rémi Galland, Jie Gao, Irene Wang, Meike Kloster, Antoine Delon, Univ. Joseph Fourier (France); Robert K. Henderson, The Univ. of Edinburgh (United Kingdom) . . . . . . . . . [8086-27]A closed-loop feedback system is introduced for automated multifocal laser alignment using a LCOS-SLM and a 32x32 pixel CMOS SPAD array, suitable for high throughput confocal FCS and FLIM applications.





SESSION 6

Room: Room 5 Mon. 14.00 to 15.30

Molecular Imaging and Novel Geometries II

Session Chair: Nanguang Chen, National Univ. of Singapore

(Singapore)

SESSION 6

Room: Room 11 Mon. 14.00 to 15.30

Optical Tomography IISession Chairs: Subhadra

Srinivasan, Dartmouth College (USA); Hamid Dehghani, The Univ. of Birmingham (United

Kingdom)

SESSION 4

Room: Room B13 Mon. 14.00 to 15.30

Photoacoustic Techniques

Session Chair: Henricus J. C. M. Sterenborg, Erasmus MC

(Netherlands)

SESSION JS1

Room: 13b Mon. 14.00 to 15.30

Medical Imaging: Joint Session with E-CLEO

Session Chairs: Peter E. Andersen, Technical Univ. of Denmark (Denmark); Monika A. Ritsch-Marte, Innsbruck

Medical Univ. (Austria)

14.00: Label-free live brain imaging with third-harmonic generation microscopy (Presentation Only), Stefan M. Witte, Vrije Univ. Amsterdam (Netherlands) . . . . . . . . [8091-500]14.30: Optical coherence tomography imaging: technology and applications (Presentation Only), James G. Fujimoto, Massachusetts Institute of Technology (USA) . . . . [8091-501]Optical coherence tomography (OCT) is a new imaging modality which generates cross-sectional a n d 3 D i m a g e s o f t i s s u e pathology. OCT utilizes advanced photonics technologies and has clinical applications ranging from ophthalmology to cardiology. This tutorial describes OCT technology and applications.

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8091: Room 13BOptical Coherence Tomography and

Coherence Techniques V

14.00: Intravascular photoacoustic imaging of human coronary atherosclerosis, Krista Jansen, Antonius F. W. van der Steen, Heleen M. M. van Beusekom, J. Wolter Oosterhuis, Gijs van Soest, Erasmus MC (Netherlands) . . . . . . . . . [8090-18]We demons t ra te i n t r avascu la r photoacoustic imaging of human coronary atherosclerotic plaque ex vivo, using a custom-built 1.25 mm diameter imaging catheter. Specific photoacoustic imaging of lipid content is achieved by spectroscopic imaging at different wavelengths between 1180 and 1230 nm.

14.00: Hyperspectral fluorescence tomography of quantum dots using the simplified spherical harmonics equations, Alexander D. Klose, Columbia Univ. (USA) . . . . . . [8088-30]The proposed image reconstruction method exploi ts the spectra l ly-dependent absorption properties of biological tissue and quantum dots for recovering the three-dimensional reporter distribution. Only a single light source with macro-illumination needs to be used for the purpose of light emission stimulation and image reconstruction. The light propagation in strongly absorbing tissue is modeled with the SPN equations.

14.15: Toward absolute quantification in CW-fDOT systems: use of a priori information, Anne Planat-Chrétien, Anne Koenig, Jean-Guillaume Coutard, Lionel Hervé, Marco Brambilla, Jean-Marc Dinten, Commissariat à l’Énergie Atomique (France) . . . . . . . . . .[8088-31]In the context of continuous wave fluorescence-enhanced diffuse optical tomography, we demonstrate how a priori knowledge of the optical parameters can lead to absolute quantification. Simulation quantitative results are provided.

14.00: 3D tumor imaging by self interference fluorescence endoscopy (Invited Paper), Mattijs de Groot, Johannes F. de Boer, Vrije Univ. Amsterdam (Netherlands) . . . [8086-28]W e p r e s e n t S e l f I n t e r f e r e n c e Fluorescence Endoscopy (SIFE), a novel technique that allows depth localization of fluorescent markers. The technique, which does not require axial scanning, uses a phase plate to introduce a modulation on the fluorescence spectrum. The phase of this modulation uniquely determines the depth location of the source.

14.15: Signal content windowing for SNR improvement in photoacoustic imaging, Pieter Kruizinga, Erasmus MC (Netherlands); Seungsoo Kim, Salavat R. Aglyamov, The Univ. of Texas at Austin (USA); Gijs van Soest, Ton van der Steen, Erasmus MC (Netherlands); Stanislav Y. Emelianov, The Univ. of Texas at Austin (USA) . . . . . . [8090-19]A new signal content windowing (SCW) method is proposed to improve the signal-to-noise ratio of photoacoustic recordings. The superiority of the SCW method over normal averaging is shown by comparing the improvement of SNR using synthetic and experimental photoacoustic data.

14.30: Photoacoustic section imaging with an integrating cylindrical detector, Sibylle Gratt, Klaus Passler, Robert Nuster, Günther Paltauf, Karl-Franzens-Univ. Graz (Austria) [8090-20]A piezoelectric detector with cylindrical shape for photoacoustic section imaging is characterized. The resolution of the detector was estimated in directions perpendicular and parallel to the detection plane and a biological sample was imaged.

14.30: Development of image reconstruction algorithms for fluorescence diffuse optical tomography using total light approach, Shinpei Okawa, Yukio Yamada, The Univ. of Electro-Communications (Japan); Hiroaki Yamamoto, FUJIFILM Corp. (Japan); Yoshihiro Miwa, Univ. of Tsukuba (Japan) . . . . . . . . . . . . . . . . . . .[8088-32]Fluorescence diffuse optical tomography using the total l ight approach is developed. The continuous wave light is used for excitation in this system. The reconstruction algorithm reconstructs the absorption coefficients increased by the fluorophore. Additionally we propose the algebraic reconstruction technique with noise reduction. Some numerical and phantom experiments are shown.

14.30: Single molecule microscopy: from nanodiamonds to nanomanipulation, Felix Koberling, Marcelle König, Benedikt Krämer, Volker Buschmann, Michael Wahl, Rainer Erdmann, Uwe Ortmann, PicoQuant GmbH (Germany) [8086-15]We wil l show results on photon coincidence measurements of NV defect centers in single nanodiamonds and we will present different techniques to overcome the optical diffraction limit: a combination of a confocal microscope with an AFM and a chemical approach to control the fluorescence blinking of single molecules.



SESSION 6 continued14.45: Multi-wavelength diffusive optical tomography using independent component analysis and time reversal algorithms, Mohammad Alrubaiee, Binlin Wu, The City College of New York (USA); Min Xu, Fairfield Univ. (USA); Wei Cai, Swapan K. Gayen, The City College of New York (USA) . . . . . . . . . . . . . . . . . . . [8088-33]Optical imaging and three-dimensional localization of two tumors embedded in a realistic model breast was accomplished using multiple-wavelength near-infrared light probing, multi-detector signal acquisition, and independent component analysis and time reversal algorithms for information retrieval.

SESSION 4 continued14.45: Enlarged acceptance angle of a finite-size detector in photoacoustic imaging using acoustic lenses, Wenfeng Xia, Daniele Piras, Michelle Heijblom, Univ. Twente (Netherlands); Spiridon Van Veldhoven, Christian Prins, Oldelft Ultrasound B.V. (Netherlands); Johan Van Hespen, Wiendelt Steenbergen, Ton Van Leeuwen, Srirang Manohar, Univ. Twente (Netherlands) . . . . . . [8090-21]We present a new acoustic lens material for use in photoacoustic imaging. The material has excellent lensing properties, small acoustic impedance mismatch with tissue and a low acoustic attenuation. We demonstrate its superior properties compared to standard lens materials using simulation and experiment.

15.00: Multispectral optoacoustic tomography (MSOT) for preclinical imaging of the cardiovascular system, Adrian Taruttis, Eva Herzog, Daniel Razansky, Vasilis Ntziachristos, Helmholtz Zentrum München GmbH (Germany) . . . . . . . . . . . . . . . [8090-22]We demonstrate in-vivo results using Multispectral Optoacoustic Tomography MSOT) to noninvasively image disease-relevant cardiovascular structures in mice in real-time, an unprecedented ability in optical imaging approaches documented so far. Using multispectral excitation, MSOT allows visualization of blood oxygenation levels and optical probes based on fluorescent dyes or absorbing nanoparticles.

SESSION 6 continued14.45: Optically sectioned imaging by oblique plane microscopy, Sunil Kumar, Dean Wilding, Markus B. Sikkel, Alexander R. Lyon, Ken T. MacLeod, Christopher W. Dunsby, Imperial College London (United Kingdom) . . . . . . . . . . . . . . . . [8086-30]An optically sectioning microscopy technique based on oblique selective plane illumination combined with oblique imaging is presented. The same high numerical aperture lens is used to both illuminate and image the specimen. The system is applied to imaging calcium wave activity in live cardiac myocytes at 500 fps.

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15.00: Toward 3-dimensional virtual biopsy of oral lesions through the development of a confocal endomicroscope interfaced with embedded computing, Patricia S. P. Thong, National Cancer Ctr. of Singapore (Singapore); Malini C. Olivo, National Cancer Ctr. of Singapore (Singapore) and Singapore Bioimaging Consortium (Singapore) and National Univ. of Ireland Galway (Ireland); Muhammad Movania, Stephanus S. Tandjung, Nanyang Technological Univ. (Singapore); Ramaswamy Bhuvaneswari, National Cancer Ctr. of Singapore (Singapore); Hock-Soon Seah, Feng Lin, Kemao Qian, Nanyang Technological Univ. (Singapore); Khee-Chee Soo, National Cancer Ctr. of Singapore (Singapore) and Singapore General Hospital (Singapore) [8086-31]Confocal laser endomicroscopy is an emerging technique that holds p r o m i s e f o r v i r t u a l b i o p s y i n disease diagnosis. We developed a prototype real-time 3-dimensional (3D) fluorescence imaging system using a confocal laser endomicroscope interfaced with embedded computing. Results demonstrate the potential of 3D fluorescence imaging for virtual biopsy of oral lesions.

15.15: Multi channel spectrally encoded endoscopic probe, Guy Engel, Dvir Yelin, Technion-Israel Institute of Technology (Israel) [8086-32]Imaging using a novel miniature endoscopic probe, which utilizes a single multi-mode fiber illumination and a single-mode fiber for spectrally encoded imaging, is experimentally demonstrated. By continuously moving the illumination fiber and averaging over subsequent frames, speckle noise was effectively eliminated from an image of a resolution test target.

15.15: Classification of optical tomographic images of arthritic fingers with quadratic discriminate analysis, Ludguier D. Montejo, Hyun-Keol Kim, Yrjö Häme, Jingfei Jia, Columbia Univ. (USA); Julio D. Montejo, Harvard Univ. (USA); Uwe J. Netz, Laser- und Medizin-Technologie GmbH, Berlin (Germany); Sabine Blaschke, Paul A. Zwaka, Universitätsmedizin Göttingen (Germany); Gerhard A. Müeller, Universitätsmedizin Göttingen (USA); Jürgen Beuthan, Charité Universitätsmedizin Berlin (Germany); Andreas H. Hielscher, Columbia Univ. (USA) . . . . . . . . . . . . . . . . . . . [8088-35]Quadratic discriminate analysis of FDOT absorption and scattering images is used to classify patients as affected or not affected by rheumatoid arthritis. The highest Youden (Y) index obtained when absorption and scattering images are combined is Y=0.75, while Y=0.65 and Y=0.70, when absorption or scattering were used separately, respectively

15.15: Image-quality enhancement in optoacoustic tomography with flat detectors, Amir Rosenthal, Daniel Razansky, Vasilis Ntziachristos, Helmholtz Zentrum München GmbH (Germany) . . . . . . . . . . . . . . . [8090-23]In this work we study the distortions caused to optoacoustic signals by the size of flat detectors and the resulting reconstruction errors and resolution loss in 2D tomographic geometries and demonstrate how they can be overcome. The analysis is performed using an analytical solution for the impulse response of a flat detector and our recently developed interpolated model matrix inversion (IMMI) algorithm. The spatially dependent impulse response of the detector is integrated into IMMI using temporal convolution, and can thus be taken into account in the inversion. We show that when sufficient projections are available, the reconstruction artifacts can be eliminated and the resolution can be enhanced.



Posters - MondayRoom: ICM Foyer Ground Floor · Mon. 15.30 to 17.00

Presenters may put up their posters Monday morning before the lunch/poster viewing break. Poster authors must be at their posters during the poster session from 15.30 to 17.00, and should take down posters immediately following this session. Coffee will be provided.

Conference 8086Advanced Microscopy

Techniques IISession Chairs: Peter T. C. So, Massachusetts Institute

of Technology (United States); Emmanuel Beaurepaire, Ecole


High-speed focal modulation microscopy using acousto-optical modulators for observation of thick biological specimens, Shau Poh Chong, Guangjun Gao, Colin J. R. Sheppard, Nanguang Chen, National Univ. of Singapore (Singapore) . . . . . . . . . . . . . . [8086-36]Focal modulation microscopy (FMM) is an advanced fluorescence technique which effectively rejects the out-of-focus fluorescence background that arises when imaging deep inside strongly scattering media. Recently, we demonstrated FMM using acousto-optical modulators (AOM) which enables MHz range modulation frequency for high-quality real-time visualization of thick biological specimens.

Pulse laser assist optical tweezers (PLAT) with long-duration pulse laser, Saki Maeda, Tadao Sugiura, Kotaro Minato, Nara Institute of Science and Technology (Japan) . . . . . . . . . . . . . . . . . . [8086-37]We have developed a technique on optical tweezers with pulse laser beam to assist conventional optical tweezers for “in vivo manipulation” and have successfully demonstrated to manipulate objects adhered on surface of a living cell. We report availability of pulse laser assistance with a newly developed long-duration pulse laser.

Angular resolved scattering microscopy, Thomas Rothe, Michael Schmitz, Alwin Kienle, Univ. Ulm (Germany) . . . . . . . . . . . . . . . [8086-38]Using an angular resolved scattering microscopy we are able to distinguish diameters of single polystyrene spheres with a standard deviation of less than 1%. The results are in very good agreement with previous measurements which were done with a spectral resolved scattering microscope and a collimated transmission setup as well.Comparison between spectral resolved scattering microscopy and collimated transmission measurements, Michael Schmitz, Thomas Rothe, Alwin Kienle, Univ. Ulm (Germany) . . . . . . . . . . . . . . . [8086-39]The mean diameter and the standard deviat ion of di luted polystyrene beads are determined by a collimated transmission setup. Afterwards a significant amount of these beads is analyzed separately by a spectral resolved scattering microscope.

High-speed molecular detection and analysis using DVD optical disc technology, Junji Tominaga, Subash C. B. Gopinath, National Institute of Advanced Industrial Science and Technology (Japan); Osamu Nagumo, Design Tech Co., Ltd. (Japan); Emi Suenaga, Penmetcha K. R. Kumar, National Institute of Advanced Industrial Science and Technology (Japan) . . . . . . . . . . . . . . . . . [8086-40]

Specially designed RNAs and aptamers are functional nucleic acids to strongly combine their target molecules and proteins. These reactions have been monitored by filtering binding assay or by surface plasmon technique so far. Here, we provide alternative cheap and useful optical bio-molecular sensor media and equipment based on an optical disc technology.

Characterization the correlation between collagen fibril thickness and forward and backward second harmonic signal, Chiu-Mei Hsueh, Vladimir A. Hovhannisyan, Chen-Yuan Dong, National Taiwan Univ. (Taiwan) . . . . . . . . . . . . . . . . . [8086-41]The purpose of this study is to image and quantify the collagen fibril thickness by second harmonic generation (SHG) microscopy and to investigate its potential as being a clinical in vivo monitoring technique for collagen related disease progress.Simultaneous reflection microscopy and surface plasmon resonance imagery: dynamical study of cell motility, Julien Moreau, Lab. Charles Fabry (France); Jean-Marc Allain, Ranjit Gulvady, Ecole Polytechnique (France); Alain Bellemain, Michael T. Canva, Lab. Charles Fabry (France) . . . . . [8086-42]W e p r e s e n t a n o v e l o p t i c a l i n s t r u m e n t a t i o n w h i c h a l l o w s simultaneous reflection microscopy and surface plasmon resonance imagery. The aim is to obtain near-field as well as far field information in real time. A study on cell motility on the model case of Dictyostelium cells was initiated using this setup.

Dynamic analysis of pulmonary infection using coupled ventilator and two-photon microscope, Daniel Fiole, Institut de Recherche Biomédicale des Armées (France) and Univ. Joseph Fourier (France); Anne Quesnel-Hellmann, Aurélie Cleret, Kévin Garraud, Jacques Mathieu, Institut de Recherche Biomédicale des Armées (France); Jean-Claude A. Vial, Julien Douady, Univ. Joseph Fourier (France); Jean-Nicolas Tournier, Institut de Recherche Biomédicale des Armées (France) . . . . . . . . . . . . . . . . . [8086-43]Two-photon imaging coupled to mechanic ventilation allows in vivo visualization of lung immune system dynamic under infection and pathogen uptake by immune cells, such as dendritic cells, in mice.

Lensfree in-line holographic detection of bacteria, Vincent Poher, Cedric P. Allier, Jean Guillaume Coutard, Jean Marc Dinten, Lab. d’Electronique de Technologie de l’Information (France) . . . . . . [8086-44]We report a new method based on a thin wetting film to detect single bacteria with a lensless imaging system. The film produces a micro-lens effect on top of each bacterium when the sample dries up. We achieve detection rate up to 95% with micron-sized beads at high object density.

4D confocal microscopy method for drug localization in the skin, Ulf Maeder, Thorsten Bergmann, Jan Michael Burg, Sebastian Beer, Peggy Schlupp, Thomas Schmidts, Johannes T. Heverhagen, Frank Runkel, Martin Fiebich, Fachhochschule Giessen-Friedberg (Germany) . . . . . . . [8086-45]

A 4D confocal microscopy (xyzλ) method for measuring the drug distribution in skin samples after a permeation study is investigated. Measuring the emission spectra allows the evaluation of a fluorescence-marked agent in the skin. The method is robust against signal decrease due to the optical properties and the skin auto-fluorescence spectra.

Simultaneous SHG and TPEF imaging using a new type of selective markers, Evelien De Meulenaere, Katholieke Univ. Leuven (Belgium); Sotiris Psilodimitrakopoulos, ICFO - Instituto de Ciencias Fotónicas (Spain); Stijn J. Van Cleuvenbergen, Jozef Vanderleyden, Katholieke Univ. Leuven (Belgium); Pablo Loza-Alvarez, ICFO - Instituto de Ciencias Fotónicas (Spain); Koen Clays, Katholieke Univ. Leuven (Belgium) . . . . . . . . . . . . . . . . [8086-46]We des igned, synthes ized and character ized a number of new fluorescent dyes with high performance in Second Harmonic Generation (SHG) Imaging. We studied cell toxicity and bleaching of the dyes, and prove that the dyes are selective for use in cellular imaging both for fluorescence and SHG.

2-photon based calcium imaging in rat ventricular myocytes using Indo-1, Benjamin Sauer, Peter Lipp, Lars Kaestner, Univ. des Saarlandes (Germany) . . . . . . . . . . . . . . . [8086-47]Although we faced an abundance of endogenous molecules with a signi-cant autofluorescence in the excitation wavelength range of 685 nm to 800 nm (probably flavin-adenine-dinucleotide and nicotinamide-adenine-dinucleotide) we found conditions and procedures to detect Indo-1 signals. Calcium transients could be measured with a 700 nm-excitation.

Exploiting chromatic aberration to spectrally encode depth in reflectance confocal microscopy, Kristen C. Maitland, Oscar Carrasco-Zevallos, Ryan L. Shelton, Cory Olsovsky, Meagan A. Saldua, Brian E. Applegate, Texas A&M Univ. (USA) . . . . . . . . . . . . . . . . . . . [8086-48]We present chromat ic confocal microscopy as a technique to axially scan the sample by spectrally encoding depth information to avoid mechanical scanning of the lens or sample. We have achieved an 800 µm focal shift using a hyperchromat lens over a range of 680-1080 nm using a tunable laser.

Vertical differential interference contrast using SLMs, Michael Warber, Malte Hasler, Tobias Haist, Wolfgang Osten, Univ. Stuttgart (Germany) . . . . . . . . . . . . . . . [8086-49]Results of different common phase contrast methods that are realized on a microscope equipped with a SLM are presented. By changing the phase contrast filter or its parameters, it is possible to considerably improve the imaging performance. Furthermore, using the possibility to change the phase of the SLM, new phase contrast methods have been developed.

Intravital confocal and two-photon imaging of dual-color cells and extracellular matrix mimics, Ufuk Bal, Ege Univ. (Turkey); Urs Utzinger, The Univ. of Arizona (USA) . . . . . . [8086-50]

We studied confocal backscattering, SHG and autofluorescence as source of contrast in extracellular matrix mimic imaging. Signal attenuation and structural contrast is discussed for commonly used matrix gels. For imaging RFP and GFP labeled cells in 3D constructs we present results of photo bleaching with confocal and two-photon excitation.

Exploring the interactions between peptides and lipid bilayers using coherent anti-stokes Raman scattering and two-photon fluorescence, Meropi Mari, The Univ. of Edinburgh (United Kingdom) . . . . . . . . . . . . . . . . [8086-51]We combine Coherent Anti-Stokes Raman Scattering (CARS) with Two Photon Excitation Fluorescence (TPEF) using a Nd: YVO4 pump laser to study the toxicity of beta Amyloid (Aβ) on lipid bilayers. The GUVs imaging by CARS and TPEF microscopy not only permits the direct imaging of the leakage phenomenon caused by the toxic peptide (Aβ) on the lipid bilayer (pore formation lysis), but also records simultaneously the lateral structure of the bilayer and peptide distribution in the plane across the membrane.

Multilayer coated echelle transmission gratings for x-ray phase sensitive imaging, Susanna K. Lynch, Nicole Morgan, Camille Kemble, Eric Bennett, National Institutes of Health (USA); Xianghui Xaio, Argonne National Lab. (USA); Harold H. Wen, National Institutes of Health (USA) . . . [8086-52]A grating design is proposed that uses an echelle pattern with multilayer deposition and angled x-ray transmission to produce higher grating density than is presently achievable. A prototype grating was tested at APS synchrotron source and the results are presented.

A novel compact digital scanning microscope, Hong Chou Lyu, Hsing Cheng Yu, Kuen Chiuan Cheng, Yuan Chin Lee, Jau Jiu Ju, Industrial Technology Research Institute (Taiwan) . . . . . . . . . . . . . . . . . [8086-53]Digital scanning microscope (DSM) for observing cellular fluorescent micro-images is proposed and manufactured. The DSM has been designed based on the concept of the optical pick-up head (PUH); thus, DSM has a blue-ray PUH module with a tri-axial scanning actuator (TSA) system. The PUH module can work with time-correlated single photon counting (TCSPC) module and serve as a DSM for detecting fluorescent image.

A reconstruction method for linear sensor microscopy based on improvement of lateral resolution isotropy, Milton P. Macedo, Instituto Superior de Engenharia de Coimbra (Portugal); Carlos M. Correia, Univ. of Coimbra (Portugal) . . . . . . . . [8086-54]We describe the implementation of a reconstruction method that combine algorithms to improve lateral resolution isotropy of 2D images and those to build 3D images. Overall system and experimental determination of Strehl ratios of 0.62 and 0.96 for wide-field and slit-illumination configurations are depicted. The results show the enhancement of depth discrimination.

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Conference 8088Diffuse Optical

Imaging IIISession Chairs: Paola Taroni,

Politecnico di Milano (Italy); Andreas H. Hielscher, Columbia

Univ. (United States)

Depth-resolved quantitative measurement of cerebral blood flow using broad-band near infrared spectroscopy and a two-layer head model, Vladislav Y. Toronov, Ryerson Univ. (Canada); Jonathan T. Elliott, Ting-Yim Lee, Keith St. Lawrence, The Univ. of Western Ontario (Canada) . . . . . . . . . . . . . . . . [8088-55]



A new device for registration of bacterial cells, Oleksandr I. Bilyy, Vasyl B. Getman, Roman Y. Yaremyk, Yaroslav P. Ferensovich, Ivan Franko National Univ. of L’viv (Ukraine); Ihor Y. Kotsyumbas, Ihor M. Kushnir, State Scientific-Research Control Institute of Veterinary Preparations and Fodder Additives (Ukraine) . . . . . . . . [8086-55]A new device for registration of bacterial cells is described. There possibilities of device from development of technologies of registration and visualization of measuring results on the bacterial cells of different nature are present.

Thin gold films as contrast agents and their potential applications, Jing Zhang, Yu Huang, Suejit Pechprasarn, Mark C. Pitter, Michael G. Somekh, The Univ. of Nottingham (United Kingdom) . . . . . . . . . . . . . . . . [8086-56]Experiments and simulation demonstrate that gold films less than 20 nm can be used as a contrast-improving agents. The morphology of the metallic surface enhances the local field and is very sensitive to variations of the environment close or attached to the surface, hence it is suitable for binding studies.

Calibration phantom for the quantification of fluorescent labels in deep skin tissue, Sebastian Beer, Ulf Maeder, Thorsten Bergmann, Peggy Schlupp, Thomas Schmidts, Fachhochschule Giessen-Friedberg (Germany); Johannes Heverhagen, Philipps Univ. Marburg (Germany); Frank Runkel, Martin Fiebich, Fachhochschule Giessen-Friedberg (Germany) . . . . . . . . . . . . . . . [8086-57]A way of quantifying fluorescent labeled drugs in deep skin tissue using confocal microscopy is presented. For the indispensable calibration, a phantom which resembles the optical and chemical properties of the skin is used. As the phantom is, unlike the skin, a homogenous and reproducible sample, calibration for different fluorophores using the phantom is possible.

Design of Temporal focusing endomicroscope for scanless, depth resolved, high sensitivity wide field imaging, Heejin Choi, Peter T. C. So, Massachusetts Institute of Technology (USA) . . . . . . . . . . . . . . . . . . . [8086-58]

Post-DeadlineQuantitative phase contrast imaging of red blood cells using digital holographic microscopy, Nimit Patel, Vandana Sinha, Maharaja Sayajirao Univ. of Baroda (India); Vani Chhaniwal, University of Stuttgart (Germany); Arun Anand, Maharaja Sayajirao Univ. of Baroda (India); Giancarlo Pedrini, Wolfgang Osten, University of Stuttgart (Germany); Bahram Javidi, University of Connecticut (United States) . [8086-59]Quantitative phase contrast imaging of red blood cells is achieved using off-axis Digital holographic interferometric microscopy. Dynamics of RBC is studied by evaluating the phase contrast images. The measured amplitude and frequency of oscillations were 300nm and 0.005Hz respectively.

Frequency domain diffuse fluorescence tomography for detection of deep lesions, Uwe J. Netz, Ingo Gersonde, Jan Toelsner, Gerd Illing, Laser- und Medizin-Technologie GmbH, Berlin (Germany) . . . . . . . . . . . . . . . [8088-60]Frequency domain fluorescence imaging in backscattering geometry allows for three-dimensional localization of lesions marked with a fluorescing dye. Tissue like optical phantoms with fluorescing inclusions of different sizes and different fluorophor concentrations were used to investigate the dependence on depth, diameter and modulation frequency in detection of fluorophor marked lesions.

Uncertainty analysis for fluorescence tomography with Monte Carlo method, Alice Köstinger, Manuel Freiberger, Hermann Scharfetter, Technische Univ. Graz (Austria) . . . . . . . . . . . . . . . . . [8088-62]It is difficult to estimate the uncertainty of reconstructed parameters in case of iterative algorithms and a large number of degrees of freedom. A Markov chain Monte Carlo method was used to explore the uncertainties of the fluorophore concentration and life-time in fluorescence tomography.

Using the topological derivative for initializing a Markov-chain Monte-Carlo reconstruction in fluorescence tomography, Manuel Freiberger, Technische Univ. Graz (Austria); Antoine Laurain, Michael Hintermüller, Univ. Graz (Austria); Alice Köstinger, Hermann Scharfetter, Technische Univ. Graz (Austria) . . . . . . . . . . . . . [8088-63]The topological derivative estimates the effect of infinitely small perturbations on the measurement data. From the outcome it is possible to estimate the number of inclusions and their approximate locations. This result is useful for the initialization of shape-based reconstruction algorithms such as the level-set method or a Monte-Carlo method.

Numerical modeling of light propagation in biological tissues: time-resolved 3D simulations based on light diffusion model and FDTD solution of Maxwell’s equations, Noé Ortega-Quijano, Univ. de Cantabria (Spain); Oleg G. Romanov, Belarusian State Univ. (Belarus); Félix Fanjul-Vélez, Irene Salas-García, Univ. de Cantabria (Spain); Alexei L. Tolstik, Belarusian State Univ. (Belarus); José L. Arce-Diego, Univ. de Cantabria (Spain) . . . . . . . . . . . . . . . . . . [8088-64]In this work, pulsed light propagation through biological tissues is analyzed by FDTD method. We perform a time-resolved study of pulsed light propagation as a function of the scatterers characteristics. The results can be applied to aspects like ultrashort light pulses propagation or the effect of modified wavefronts.

Recent progresses on video-rate 3D nonlinear optical imaging, Masood Samim, Virginijus Barzda, University of Toronto (Canada) . . . . . . . . . [8086-60]Recent developments towards high-speed imaging capabil i t ies have gained significant notice in the field of microscopy. Multi-contrast, multi-foci nonlinear optical microscopes are the most recent examples. We employ a Xilinx Vertix-5 FPGA board, membrane deformable mirrors and high-speed galvanometer/scanning mirrors to achieve the high-speed imaging. We demonstrated the ability of the 39-actuator deformable mirrors in reshaping the phasefront and correcting for the optical aberrations. Here, we present the design of this novel microscope and data illustrating the ability to focus at distinct axial depths. The 3D video-rate scanning capability is an important tool for the study of rapid dynamics of bio-organisms.

Self organized polymer microlenses for sub-diffraction limit optical microscopy, Ankur Verma, Ashutosh Sharma, Indian Institute of Technology Kanpur (India) . . . . . . . . . . . . [8086-61]Polystyrene microlenses and their arrays are fabricated by self organized dewetting of ultrathin films in aqueous media. These lenses are used to resolve 200 nm wide blue-ray DVD tracks using bright field optical microscope using 50x objective (NA/0.8).

Screening of potential endocrine disruptors for estrogen receptor by means of fluorescence polarization and total internal reflection fluorescence, Kwok-wing Yiu, Hong Kong Baptist University (Hong Kong, China) . . . . . . . . . . . . . . . . . . [8086-62]This thesis reports the measurement of binding strength and binding kinetics between human estrogen receptor and different kinds of endocrine disruptors by means of fluorescence polarization and total internal reflection fluorescence. Our results show that the equilibrium dissociation constants for different endocrine disruptors ranged from ~1nM to ~100µM and their off-rates ranged from ~1.5 x 10-3 to ~30 x 10-3 s-1. We also found that mixture of endocrine disruptors (PBA, PFOS. PFNA and PFOA) did not show synergistic binding with the endocrine disruptor.

Complications that disrupt blood flow to the brain and exacerbate neurological injury often go undetected because no adequate bedside measure of cerebral blood flow (CBF) exists. We propose an algorithm based on a two-layer optical model to quantify CBF from dynamic contrast-enhanced near-infrared data acquired with a two-channel broadband system.

Dual-modality small animal bioluminescence/diffuse optical tomography system, James A. Guggenheim, Hamid Dehghani, Hector Basevi, Iain B. Styles, Jon Frampton, The Univ. of Birmingham (United Kingdom) . . . . . . . . . . . . . . . . [8088-56]Development and validation of a new combined bioluminescence and optical tomography imaging system for in vivo small animal studies. Novel multi-view data collection and image reconstruction strategies are presented, producing 3D volumetric maps of molecular source distributions for multiple markers.

Estimation of the responsivity of detection systems in diffuse tissue optics, Heidrun Wabnitz, Dieter R. Taubert, Oliver Steinkellner, Mikhail Mazurenka, Alexander Jelzow, Physikalisch-Technische Bundesanstalt (Germany); Alessandro Torricelli, Davide Contini, Lorenzo Spinelli, Politecnico di Milano (Italy); Piotr Sawosz, Daniel Milej, Adam Liebert, Institute of Biocybernetics and Biomedical Engineering (Poland); Rainer Macdonald, Physikalisch-Technische Bundesanstalt (Germany) . . . [8088-57]The responsivity of detection systems of time-domain brain imagers, i.e. the overall efficiency to detect light emerging from tissue, is measured using dedicated solid slab phantoms. The wavelength-dependent diffuse transmittance factor of these phantoms was determined quantitatively by radiometric measurements. The method can be extended to other photon migration instruments.

Principal and independent component analysis of concomitant functional near infrared spectroscopy and magnetic resonance imaging data, Vladislav Y. Toronov, Irina Schelkanova, Ryerson Univ. (Canada) . . . . . . . . . . . . . . . . [8088-58]We found that principal and independent component analysis of the broadband near infrared spectroscopy data allows separating different spatial and physiological sources of functional cerebral signals.

Determination of the reduced scattering and absorption coefficients by spatially resolved reflectance and total reflectance measurements, Florian Foschum, Marie-Theres Heine, Alwin Kienle, Univ. Ulm (Germany) . . . . . . . . . . . [8088-59]We present a combination of two CW methods, measurement of the spatiallyresolved reflectance and of the total reflectance, for determination of the reduced scattering and absorption coefficients. The results are compared with independent methods and show excellent agreement.

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Performance evaluation of hybrid FMT-XCT reconstruction with XCT based forward modeling, Pouyan Mohajerani, Angelique B. F. Ale, Vasilis Ntziachristos, Helmholtz Zentrum München GmbH (Germany) and Technische Univ. München (Germany) . . . . . . . . . . . . . . . [8089-22]A f r amework was s tud ied fo r reconstruction of the fluorescence distribution in small animals, measured using the FMT-XCT system. The framework accurately models the system while handling memory and speed constraints, hence facilitating the imaging of challenging animal models.

Small animal fluorescence imaging with lifetime and spectral resolution for detection of RFP-expressed tumors, Mikhail S. Kleshnin, Ilya V. Turchin, Ilya I. Fiks, Institute of Applied Physics (Russian Federation); Irina V. Balalaeva, N.I. Lobachevsky State Univ. of Nizhni Novgorod (Russian Federation); Alexander P. Savitsky, A.N. Bach Institute of Biochemistry (Russian Federation) . . . . . . . . . . . . . . [8089-23]We present the two setups for small animal fluorescence imaging. The first one combines reflectance technique with lifetime resolution, and the another one intended for spectrally resolved diffuse fluorescent tomography. The results of in vivo study with RFP expressed tumors will be reported.

Coronary wall imaging in mice using osmium and micro-computed tomography, Megan Kozlowski, National Heart Lung and Blood Institute (USA); Danielle Donohue, National Institute of Neurological Disorders and Stroke (USA); Mathew Daniels, Patricia Connelly, Kenneth Jeffries, Randall Clevenger, Han H. Wen, Vinay M. Pai, National Heart Lung and Blood Institute (USA) . . . . . . . . . . . . . . . . . . . [8089-24]Coronary artery disease, a major cause of death in the US, results from the accumulation of atherosclerotic plaques in the coronaries. However, there is debate over the development (or atherogenesis) of these plaques. We present a coronary artery wall imaging approach combining osmium and microcomputed tomography for helping address this debate.

Multispectral photoacoustic mapping of the diffusion of bacteriochlorins through the skin: exploring a new PAT contrast agent, Fábio Schaberle, Gonçalo Sá, Carlos Serpa, Artur R. Abreu, Mariette M. Pereira, Luis G. Arnaut, Univ. de Coimbra (Portugal) . . . . . . . . . . . . . . . . [8089-25]A skin depth map was built reconstructing photoacoustic signals, in a backward set up, using several wavelengths of visible light. The apping technique was used to follow the diffusion through the skin of near-infrared absorbing dyes. Such dyes can be useful for photodynamic therapy (PDT) of skin lesions or as contrast agents for photoacoustic tomography (PAT), because they strongly absorb light at wavelengths where the skin is more transparent.



Spectral unmixing using component analysis in multispectral optoacoustic tomography, Stefan Morscher, Jürgen Glatz, Nikolaos C. Deliolanis, Andreas Buehler, Athanasios Sarantopoulos, Daniel Razansky, Vasilis Ntziachristos, Helmholtz Zentrum München GmbH (Germany) and Technische Univ. München (Germany) . . . . . . . . . . . . . . . [8089-26]Multispectral optoacoustic tomography combines high ultrasound resolutions with deeply penetrating laser illumination in the near infrared. By excitation in multiple wavelengths biomarkers with different spectral absorption profiles can be recovered using a set of methods described in this work, Independent Component Analysis being the most promising option.

Conference 8089Molecular Imaging III

Session Chairs: Charles P. Lin, Wellman Ctr. for Photomedicine

(United States); Vasilis Ntziachristos, Helmholtz Zentrum

München GmbH (Germany) and Technishe Univ. München

(Germany)

Recovery of fluorescence molecular signals using a hybrid fluorescence molecular tomography and x-ray computed tomography scan, Karin Radrich, Angelique B. F. Ale, Vladimir Ermolayev, Vasilis Ntziachristos, Helmholtz Zentrum München GmbH (Germany) and Technische Univ. München (Germany) . . . . . . . [8089-19]Stand alone FMT systems lack structural information of the imaged volume which particularly affects limited view projection measurements. We show that the co-registration of data from distinct FMT and XCT devices and the subsequent use of anatomical information for FMT reconstruction signif icantly helps improving on reconstruction quality.

Optoacoustic tomography of the mouse brain using interpolated model-matrix inversion, Thomas Jetzfellner, Amir Rosenthal, Karl Hans Englmeier, Daniel Razansky, Helmholtz Zentrum München GmbH (Germany); Vasilis Ntziachristos, Helmholtz Zentrum München GmbH (Germany) and Technishe Univ. München (Germany) . . . . . . . . . . . . . . . [8089-20]Neuroscience investigations may significantly benefit from the availability of accurate imaging methods. Here we demonstrate the application of the recently introduced interpolated model-matrix inversion (IMMI)for optoacoustic tomography to image the brain of small animals. The performance showcased is superior to other non-invasive optical imaging investigations studied so far.

Evaluation of a targeted multimodality probe using human primary ovarian cancer models for the detection and monitoring of tumor growth and therapeutic response, Tracy W. B. Liu, Jocelyn Stewart, Jiyun Shi, Juan Chen, Benjamin G. Neel, Gang Zheng, Ontario Cancer Institute (Canada) . . . [8089-21]Folate receptor is highly expressed in a variety of epithelial cancers particularly ovarian cancer which is the leading cause of gynecological cancer-related mortality. We have developed a folate targeted multi-modality probe with the capacity to detect (optical and PET imaging) and treat (photodynamic therapy) primary human ovarian cancer models.

Post-DeadlineA simple low-cost dynamic breast phantom for diffuse optical imaging, Ali Hasnain, Nanguang Chen, National Univ. of Singapore (Singapore) [8088-65]In this paper, we exploit photochromic materials which change their opacity under ultra-violet (UV) light to mimic blood hemodynamics within tissue. We have designed an array of photochrome discs attached with miniature size UV LEDs, embedded in solid tissue-like phantom. The new dynamic phantom is imaged using a time-domain diffuse optical imaging system and results have been shown.

BEM-NIRFAST: open source software for three-dimensional image-guided near infrared spectroscopy using boundary element method, Subhadra Srinivasan, Hamid R. Ghadyani, Michael Jeremyn, Dartmouth College (United States) . . . . . . . . . . . . . . . . . . [8088-66]BEM-NIRFAST is open source software for near infrared (NIR) imaging using boundary element method for modeling light diffusion tissue. This toolbox requires only surface discretization of the imaging domain in 3D. The software is Matlab-based and provides a framework for surface meshing, modeling and data and solution visualization capabilities as well as ability to run in parallel environments using OpenMP standard.

Three-dimensional image-guided fluorescence using boundary element method and dual reciprocity method, Rong Yang, Subhadra Srinivasan, Robert Drysdale, Dartmouth College (United States) . . . . . [8088-67]A boundary element method with dual reciprocity method (BEM-DRM) was used to model fluorescence in tissue using coupled diffusion equations in 3D. This method eliminates the need for a node connectivity in a volume mesh. Results using BEM-DRM show agreement with data from open source finite-element based software package NIRFAST.

3D-surface reconstruction method for diffuse optical tomography phantoms and tissues using structured and polarized light, Kirstin Baum, Raimo Hartmann, Tobias Bischoff, Frank Himmelreich, Johannes T. Heverhagen, University of Marburg (Germany) . . . . . . . . . . . . . . . [8088-68]We are presenting a fast 3D-Surface reconstruction method for diffuse optical tomography phantoms and tissues using structured and polarized light.

Bio-signal analysis for the quantification of dynamic stress, Vishal Saxena, University of Southern California (United States) . . . [8088-69]Independent component analysis of NIRS signals measured with multi-distance probe configuration, Eiji Okada, Yutaka Niki, Hirokazu Kakuta, Keio Univ. (Japan); Hiroshi Kawaguchi, National Institute of Radiological Sciences (Japan) . . . . . . . . . . [8088-70]The signal component associated with the brain activation was extracted from NIRS signals measured with multi-distance probe configuration by independent component analysis (ICA). The partial optical path-length in the brain estimated by Monte Carlo simulation was compared with the coefficients of the separation matrix to evaluate the results of ICA.

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Conference 8090Novel Biophotonic


Session Chairs: Alex Vitkin, Univ. of Toronto (Canada); Henricus J. C. M. Sterenborg, Erasmus MC

(Netherlands)

Classification of the micro and nanoparticles and biological agents by neural network analysis of the parameters of optical resonance of whispering gallery mode in dielectric microspheres, Vladimir A. Saetchnikov, Elina A. Tcherniavskaia, Belarusian State Univ. (Belarus); Gustav Schweiger, Andreas Ostendorf, Ruhr-Univ. Bochum (Germany) . . . [8090-24]Network classif ier for micro and nanoparticles and biological agents in solution using such parameters of optical micro cavity resonance spectra of whispering gallery modes as spectral shift, broadening, diffuseness and others is being developed. Classification probability of approximately 98% for probes under investigation have been achieved.

Optics nanotechnology enables rapid label-free diagnostics for biomedical applications, Debra D. Wawro, Shelby Zimmerman, Resonant Sensors Inc. (USA); Robert Magnusson, The Univ. of Texas at Arlington (USA); Peter Koulen, Univ. of Missouri-Kansas City (USA) . . . . . . . . . . . . . . . . . . . [8090-25]A new highly sensitive sensor technology has the potential to simplify medical diagnostic tests by significantly reducing operation complexity compared to standard tests such as enzyme-linked immunoassays. Sensor elements operate tag-free using patient samples w i thout p re- o r post -chemica l processing. Picomolar concentrations for a wide variety of analytes can be measured.

NIR tracking assists sports medicine in junior basketball training, Roberts Paeglis, Kristaps Bluss, Biomechanics and Physical Research Institute (Latvia); Andris Rudzitis, Andris Spunde, Latvian Academy of Sports Education (Latvia); Tamara Brice, Univ. of Latvia (Latvia) . . . . . . . . . . . . . . . . . . [8090-26]We recorded eye movements of eight elite junior basketball players with an infra-red device. The research was to assist diagnostics in sports medicine in monitoring training schedule. We hypothesized that a more stable gaze is correlated to a better shot rate. We thus advocate infra-red eye tracking as a means to evaluate player selection and training success.

Time-resolved diffuse optical spectroscopy up to 1700 nm using a time-gated InGaAs/InP single-photon avalanche diode, Ilaria Bargigia, Alberto Tosi, Andrea Bahgat Shehata, Politecnico di Milano (Italy); Adriano Della Frera, Micro Photon Devices S.r.l. (Italy); Andrea Farina, Andrea Bassi, Paola Taroni, Alberto Dalla Mora, Franco Zappa, Antonio G. Pifferi, Politecnico di Milano (Italy) . . [8090-27]

So far, time-domain optical spectroscopy has been exploited mostly up to 1100 nm: we extended the spectral range from 1100 to 1700 nm via a supercontinuum laser and a time-gated InGaAs/InP Single-Photon Avalanche Diode. A first example of application is presented.

Upconversion nanosized luminophores as a novel tool for deep tissue imaging, Alexey P. Popov, Alexander Bykov, Univ. of Oulu (Finland); Andrei V. Zvyagin, Macquarie Univ. (Australia); Alexander V. Priezzhev, Lomonosov Moscow State Univ. (Russian Federation); Risto Myllylä, Univ. of Oulu (Finland) [8090-28]We report about preliminary results of using upconversion nanosized luminophores for purpose of tissue imaging. We manufactured tissue-mimicking phantoms and 10-nm particles for this study. We performed Monte Carlo simulations of fluorescence detection from an imbedded vessel and recorded nanoluminophore fluorescent spectra through transparent and scattering phantoms.

A photoplethysmography device for multipurpose blood circulatory system assessment, Edgars Kviesis-Kipge, Janis Zaharans, Oskars Rubenis, Andris Grabovskis, Univ. of Latvia (Latvia) . . . . . . . . . . . . . . . . . . [8090-29]A novel method for photoplethy-smography (PPG) signal detection has been developed and implemented in a prototype device and tested in the laboratory. The three channel digital PPG device is lightweight, portable and battery operated. The design is relatively simple, low cost and it avoids classical analog-to-digital converter chip.

Usability of photoplethysmography method in estimation of conduit artery stiffness, Andris Grabovskis, Zbignevs Marcinkevics, Lukstina Zane, Madara Majauska, Juris Aivars, Vieda Lusa, Aleksandra Kalinina, Univ. of Latvia (Latvia) . . . . . . . . . . . . [8090-30]Three channel photoplethysmography (PPG) signal waveform studies of leg conduit arteries during a provocative occlusion test were performed. Significant PPG and reference method value correlations of local and regional arterial stiffness (AS) have been observed, showing the ability to use PPG for AS change detection.

Instrumentation and method for measuring NIR light absorbed in tissue during MR imaging in medical NIRS measurements, Teemu S. Myllylä, Hannu Sorvoja, Risto Myllylä, Juha Nikkinen, Osmo Tervonen, Vesa Kiviniemi, Univ. of Oulu (Finland) . . . . . . . . . . . . . . . . . [8090-31]Our goal is to provide a cost-effective method for examining human tissue, particularly the brain, by the simultaneous use of magnetic resonance imaging (MRI) and near-infrared spectroscopy (NIRS). This paper focuses particularly on presenting the instrumentation and a method for non-invasive measuring NIR light absorbed in human tissue during MR imaging.

Ray tracing in a modified cladding fiber optic sensors, Radhi M. Chyad, Univ. Sains Malaysia (Malaysia) . . . . . . . . . . . . . . . [8090-32]Optical monitoring of beer production in real-time, Éverton S. Estracanholli, Cristina Kurachi, Igor Polikarpov, Vanderlei Salvador Bagnato, Univ. de São Paulo (Brazil) . . . . . . . . . . . . . . . . . . [8090-33]In this study we are interesting to propose a new method able to monitoring a process of beer production. Specifically, we want know in a short time of analyses, the amount of the maltose produced in the step of saccharification of the starch. For this, we propose the use of absorbance in infrared region (FTIR) and mathematical analyses as PCA (principal component analysis) and neural network.

Operating point stabilization of fiber-based line detectors for photoacoustic imaging, Karoline Felbermayer, Hubert Grün, Thomas Berer, Peter Burgholzer, RECENDT GmbH (Austria) . . . . . . . . . . . [8090-34]We introduce alternative and cost-efficient operating point stabilization for fiber-based line detectors. Thereby a fiber is bent around a piezoelectric tube and stretched by applying voltage to stabilize the operating point.

Annular piezoelectric ring array for photoacoustic imaging, Klaus Passler, Robert Nuster, Sibylle Gratt, Karl-Franzens-Univ. Graz (Austria); Peter Burgholzer, RECENDT GmbH (Austria); Günther Paltauf, Karl-Franzens-Univ. Graz (Austria) . . . . . . . . . . . . . [8090-35]A piezoelectric detection system consisting of concentric rings is investigated for large depth of field photoacoustic imaging. Compared to a single ring, the array leads to a reduction of X-shaped imaging artifacts. Image resolution studies are performed in simulations and in experiments.

Time-shifting correction in optoacoustic tomographic imaging for media with non-uniform speed of sound, Xosé L. Deán-Ben, Vasilis Ntziachristos, Daniel Razansky, Helmholtz Zentrum München GmbH (Germany) . . . . . . . . . . . . . . . [8090-36]An analysis of the t ime-shift ing correction in optoacoustic tomographic reconstructions for media with an a priori known speed of sound distribution is presented. We analyse the errors in the filtered back-projection reconstructions for discontinuities with different sizes and standard variations of the speed of sound in biological tissues.

Correction for acoustic attenuation effects in optoacoustic tomographic reconstructions, Xosé L. Deán-Ben, Daniel Razansky, Vasilis Ntziachristos, Helmholtz Zentrum München GmbH (Germany) . . . . . . . . . . . . . . . [8090-37]The feasibility of correcting for acoustic attenuation effects in optoacoustic tomographic reconstructions is shown in this work. The experimental results obtained by placing an attenuation phantom between the sample and the transducer evince the feasibility of the correction, which may also be applicable in a more general case.



Polarization-dependent optical properties: contrast enhancement in imaging of hair birefringence, Babu Varghese, Rieko Verhagen, Altaf Hussain, Qiangqiang Tai, Natallia Uzunbajakava, Philips Research Nederland B.V. (Netherlands) [8090-38]Optical properties of human hair and its interaction with polarized light are topics of interest for medical and cosmetic applications, including light-based diagnosis and treatment and for modeling of light propagation in skin and hair.

Using a polarization sensitive confocal microscope, we performed ex-vivo and in-vivo measurements on human scalp hairs and human skin and demonstrated dependence of a change in polarization of light that interacted with a birefringent hair on the orientation of the incident polarization.

F u r t h e r m o r e , w e r e f i n e d o u r understanding of the polarization-dependent optical properties of hair and demonstrated that the polarization-dependent contrast, when observed using polarization-sensitive confocal microscopy, depends on the degree of pigmentation and on the focusing depth inside the hair.

Finally, we present a method for enhancing the hair-skin contrast in images obtained using polarization sensitive confocal microscope.

Monte Carlo simulation of light reflection from cosmetic powders on the skin, Takashi Okamoto, Masafumi Motoda, Kyushu Institute of Technology (Japan); Takanori Igarashi, Keisuke Nakao, KAO Corp. (Japan) . . [8090-39]The spectrum and color of light reflected from skin covered with powder particles have been calculated. It is shown that small particles with a size less than the wavelength of light are suitable for obscuring color imperfections on the skin. The effect of particle polydispersity is also discussed.

Improving neuronavigation through workflow and sound feedback and interactive brainshift correction, Herke Jan Noordmans, Peter Woerdeman, Eduard Voormolen, Sander Van der Steen, Marijn van Stralen, Univ. Medical Ctr. Utrecht (Netherlands) . . . . . . . . . . . . . [8090-40]Different techniques are described to improve navigation in neurosurgery, like sound feedback to avoid critical structures and workflow feedback to show surgical progression, positions of interest and to interactively deform the pre-operative image data to the actual position of the cortex.

Synthesis and application of luminescent silica nanoparticles to the DNA microarray technology for improving the optical signal and the detection efficiency, Francesco Enrichi, Raffaele Ricco, Anna Meneghello, Roberto Pierobon, Erica Cretaio, Associazione CIVEN (Italy) . . . . . . . . . . . . . . . . . . . [8090-41]

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Synthesis optimization and application of luminescent silica nanoparticles to DNA microarray technology is reported. A comparison of these nanoparticles to conventional dye labelling or commercial quantum dots demonstrates a significant increase in the optical signal, and a related decrease of the limit of detection, thus giving a remarkable improvement towards early diagnosis of diseases and trace level detection of dangerous biological contaminants.

Measurement of the acoustic scatterers distribution within the imaged sample in an optoacoustic tomographic setup, Xosé L. Deán-Ben, Daniel Razansky, Vasilis Ntziachristos, Helmholtz Zentrum München GmbH (Germany) . [8090-42]We present a method to determine the position of the scatterers within the imaged sample in a tomographic optoacoustic setup and, subsequently, to reduce the ar te facts in the tomographic reconstructions due to reflection or scattering events.

Spatially-resolved measurement of Stokes parameters of partially polarized laser beams, Babu Varghese, Altaf Hussain, Siqi Ding, Qiangqiang Tai, Rieko Verhagen, Natallia Uzunbajakava, Philips Research Nederland B.V. (Netherlands) . . . . . . . . . . . . . [8090-43]There is continuously increasing interest in spatially-resolved measurement of polarization states of complex light beams for a range of applications such as bioimaging and imaging polarimetry.

Here we report on development and implementation of a simple and accurate polarimeter for measuring space variant polarization of an incident light beam. Using the constructed polarimeter we experimentally determined the Stokes parameters for five known homogeneously distributed states of polarization with accuracy higher than 98% at each transverse point in the observation plane. Furthermore, we used a spatially variable retardation plate composed of eight sectors of β/2 wave plates to create space-variant polarization and measured a nearly-radial polarization distribution in the near-field. Finally, we propose a configuration of a polarimeter that can perform real-time measurement of space variant polarizations, limited only by the frame rate of the CCD camera.

Laser speckle contrast versus depolarization: a solid skin phantom study, Lioudmila Tchvialeva, The BC Cancer Agency Research Ctr. (Canada); Gurbir Dhadwal, Diana Diao, Harvey Lui, David I. McLean, The Univ. of British Columbia (Canada); Tim K. Lee, The BC Cancer Agency Research Ctr. (Canada) . . . . . . . . . . . . . . . . [8090-44]The aim is to study how polychromatic speckle contrast and depolarization depend on roughness and bulk scattering. The experiment is done on solid skin phantoms with controllable roughness and optical properties. The speckle contrast vs. degree of linear polarization is fitted by linear regression with slope sensitive to scattering coefficient.

Post-DeadlineThermophotonic imaging of early demineralization and carious lesions in human teeth, Nima Tabatabaei, Andreas Mandelis, Univ. of Toronto (Canada); Bennett T. Amaechi, University of Texas Health Science Center (United States). . . . . . [8090-48]In this novel imaging methodology, the infrared emission from dental samples, generated by an intensity modulated laser excitation, is processed to make diagnostic images of early caries in human teeth.

Three dimensional tracking of gold nanoparticles using digital holographic microscopy, Frederic Verpillat, Fadwa Joud, Lab. Kastler Brossel (France); Michel Gross, Lab. Charles Coulomb (France) and Lab. Kastler Brossel (France); Pierre Desbiolles, Lab. Kastler Brossel (France) . . . . . . . . . . . . . . . . . [8090-49]We propose a new setup combining dark-field microscopy and digital holography to track gold nanobeads in 3D. We are able to localize 100 nm diameter particles from a single shot with a resolution smaller than the particle size. The reconstructed trajectories are consistent with the theoretical law of diffusion.

Development and confocal imaging of ZnO nanoparticles as a new photosensitizer for diagnosis and treatment of cancer using lasers, Shamaraz Firdous, Sr., 2. . . . .National Institute of Laser and Optronics, Nilore, Islamabad, Pakistan (Pakistan) . . . . . . . . . . . . . . . . [8090-50]For over ongoing Biophotonics research we have developed and characterized a cost expensive, and noninvasive Zinc Oxide (ZnO) nanoparticles (Np) of 11-15nm and nanorods(NRs) of 60-120nm diameter to use a photosensitizer in photodynamic therapy (PDT) of cancer. In the presence of a non-ionic surfactant (Tween 80) increased concentration of the surfactant was found to affect both the morphology and purity of the synthesized ZnO nanoparticles. Confocal microscopy were employed to characterize the as-prepared samples. ZnO nanoparticles ranging in particle size from 11 to 15 nm were formed at the reaction temperature of 70-80 oC.

Confocal microscopy of ALA with ZnO and ALA without ZnO significant changes were seen and verified ZnO is tissue non toxic in dark, but enhances endogenous fluorescence in HepG2 cells. The fluorescence of ZnO with ALA is prominent if compared with other images.

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SESSION 7

Room: Room 11 Mon. 17.00 to 18.00

Optical Tomography IIISession Chairs: Paola Taroni,

Politecnico di Milano (Italy); Andreas H. Hielscher, Columbia

Univ. (USA)

17.00: NIRFAST: An interactive GUI driven toolbox for modelling and image reconstruction in optical imaging (Invited Paper), Hamid Dehghani, The Univ. of Birmingham (United Kingdom); Michael Jermyn, Scott Davis, Subha Srinivasan, Venkat Krishnaswamy, Hamid R. Ghadyani, Frederic Leblond, Dartmouth College (USA); Qun Zhu, The Univ. of Birmingham (United Kingdom); Brian W. Pogue, Dartmouth College (USA) . . . . . . . . . . . . . . . . . . . [8088-36]NIRFAST (Near Infrared Fluorescence and Spectral Tomography) is a numerical model and image reconstruction interactive MATLAB toolbox based on Finite Element method (FEM) and Boundary Element method (BEM) for modelling Near-Infrared light transport in tissue. The development of this modelling and image reconstruction algorithms is a product of over 10 years development and validation which is now available to researchers as an open source package. The key features of this package are discussed and current development and future directions are outlined.

SESSION 7

Room: Room 5 Mon. 17.00 to 18.00

Embryo ImagingSession Chair: Elizabeth M. C. Hillman, Columbia Univ. (USA)


Techniques II


Imaging III

17.00: Light sheet-based fluorescence microscopy (LSFM) reduces phototoxic effects and provides new means for the modern life sciences (Invited Paper), Ernst H. K. Stelzer, European Molecular Biology Lab. (Germany) . . . . [8086-33]During the last few years, LSFM was used to record zebrafish development from the early 32-cell stage until late neurulation with sub-cellular resolution and short sampling periods (60-90 sec/stack). The recording speed was five four Megapixel large frames/sec with a dynamic range of 12-14 bit. We followed cell movements during gastrulation, revealed the development during cell migration processes and showed that an LSFM exposes an embryo to 200 times less energy than a conventional and 5,000 times less energy than a confocal fluorescence microscope (Keller 2008).

17.20: Cell lineage reconstruction of early zebrafish embryos using multiharmonic microscopy (Invited Paper), Nicolas Olivier, Ecole Polytechnique (France); Miguel A. Luengo-Oroz, Univ. Politécnica de Madrid (Spain); Louise Duloquin, Institut Federatif de Neurobiologie Alfred Fessard (France); Emmanuel Faure, Thierry Savy, Xavier Solinas, Israel Veilleux, Delphine Débarre, Paul Bourgine, Ecole Polytechnique (France); Andres Santos i Lleo, Univ. Politécnica de Madrid (Spain); Nadine Peyriéras, Institut Federatif de Neurobiologie Alfred Fessard (France); Emmanuel Beaurepaire, Ecole Polytechnique (France) . . . . . . . . . . . . . . . . . [8086-34]We used second- and third- harmonic generation (SHG, THG) microscopy associated with automated analysis to reconstruct the complete cell lineage tree of six unstained zebrafish through the first 10 division cycles.

17.40: Imaging embryos with multiphoton light sheet microscopy (Invited Paper), Willy Supatto, California Institute of Technology (USA) and Ecole Polytechnique (France); Thai V. Truong, David S. Koos, John M. Choi, Scott E. Fraser, California Institute of Technology (USA) . . . . . . . . . [8086-35]We report on the implementation and application of two-photon light sheet microscopy, combining two-photon excited fluorescence with orthogonal illumination. Using live imaging of embryos, we demonstrate the high performance of this technique compared to current state-of-the-art in terms of depth penetration, spatial resolution, acquisition speed and low phototoxicity.

17.30: 3D near-infrared imaging based on a single- photon avalanche diode array sensor (Invited Paper), Juan Mata Pavia, Univ. Hospital Zürich (Switzerland) and Ecole Polytechnique Fédérale de Lausanne (Switzerland); Edoardo Charbon, Ecole Polytechnique Fédérale de Lausanne (Switzerland) and Technische Univ. Delft (Netherlands); Martin Wolf, Univ. Hospital Zürich (Switzerland) [8088-37]An imager for optical tomography was designed based on a new 128x128 single-photon avalanche diode array sensor capable of performing time resolved measurements for each of its pixels. A new contactless setup has been conceived in which no scanning of the target is necessary.



SESSION 1

Room: B13 Tues. 08.30 to 10.30

Minimally Invasive Diagnostics/Laboratory

Medicine ISession Chair: Jürgen Popp,

Institut für Photonische Technologien e.V. (Germany)

SESSION 8

Room: Room 11 Tues. 08.45 to 10.00

Novel InstrumentationSession Chairs: Turgut Durduran, ICFO - Instituto de Ciencias Fotónicas (Spain); Xavier Intes, Rensselaer

Polytechnic Institute (USA)

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8087: Room B13Clinical and Biomedical



Imaging III

08.30: Molecular histopathology by nonlinear interferometric vibrational imaging (Invited Paper), Stephen A. Boppart, Univ. of Illinois at Urbana-Champaign (USA) . . .[8087-01]Molecular histopathology of tissue sections is performed using a rapid, label-free approach based on the generat ion and classi f icat ion of broadband vibrational spectra. Nonlinear interferometric vibrational imaging (NIVI) is a quantitative CARS-based technique that enables background-free, concentration-dependent chemical imaging for highly accurate differentiation between normal and tumor tissues.

08.45: Acousto-optic imaging using a powerful long pulse laser and digital holography, Emilie Benoit, Salma Farahi, Emmanuel Bossy, François Ramaz, Ecole Supérieure de Physique et de Chimie Industrielles (France) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .[8088-38]Acousto-optic imaging is based on ultrasound modulation of multiply scattered light in thick media. We detect the acousto-optic signal by off-axis heterodyne digital holography, which constitutes a tunable spatio-temporal filter giving a high signal to noise ratio. A powerful long-pulse laser is used to increase the optical peak power.

09.00: Photorefractive acousto-optic imaging in the therapeutic window with a conventional pulsed ultrasound scanner, Salma Farahi, Ecole Supérieure de Physique et de Chimie Industrielles (France); Alexander A. Grabar, Uzhgorod National Univ. (Ukraine); Jean-Pierre Huignard, Jphopto-Consultant (France); François Ramaz, Ecole Supérieure de Physique et de Chimie Industrielles (France) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .[8088-39]Acousto-optic imaging is based on ultrasound modulation of multiply scattered light in thick media. We demonstrate the possibility to perform a self-adaptive wave-front holographic detection at 780 nm, within the optical therapeutic window where absorption of biological tissues is minimized. Our technique represents an interesting approach for breast cancer detection.

09.15: Quantitative photoacoustic blood oxygenation measurement of whole porcine blood samples using a multi-wavelength semiconductor laser system, Claus-Stefan Friedrich, Martin P. Mienkina, Carsten Brenner, Nils C. Gerhardt, Ruhr-Univ. Bochum (Germany); Manfred Jörger, Andreas Straus, Ilias-medical GmbH (Germany); Martin F. Beckmann, Georg Schmitz, Martin R. Hofmann, Ruhr-Univ. Bochum (Germany) . . . . . . . . . . . . . .[8088-40]We present a photoacoustic measurement system based on a semiconductor lasers with four different optical wavelengths (650nm, 808nm, 850nm, 905nm) for quantitative blood oxygenation measurements. The results of a calibrated photoacoustic measurement of porcine blood samples are in good agreement with the real oxygenation values.

09.15: Evaluation of liver grafts by fluorescence spectroscopy, Rodrigo B. Correa, José D. Vollet Filho, Univ. de São Paulo (Brazil); Juliana Ferreira, Univ. do Vale do Paraíba (Brazil); Orlando Castro-e-Silva, Jr., Cristina Kurachi, Vanderlei S. Bagnato, Univ. de São Paulo (Brazil) . . . . . . . . . .[8087-02]This study aims to provide a demonstration on how fluorescence spectroscopy can be an interesting, non-invasive and useful approach to evaluate the viability of liver grafts during transplantation procedures. It includes 30 patients, for which both fluorescence and biochemical analyses were carried out.09.30: Raman spectra classification with support vector machines and a correlation kernel, Alexandros Kyriakides, Univ. of Cyprus (Cyprus); Evdokia Kastanos, Univ. of Nicosia (Cyprus); Katerina Hadjigeorgiou, Costas Pitris, Univ. of Cyprus (Cyprus) . [8087-03]The use of Support Vector Machines with a novel correlation kernel for the analysis of Raman spectra is presented. This kernel is “self-normalizing,” produces superior classification performance with minimal preprocessing, even on highly-noisy data, and its performance does not degrade when applied to distinct test sets.


SESSION JS2


Advanced Biophotonics: Sensing

and Imaging: Joint Session with E-CLEO

Session Chairs: Irene Georgakoudi, Tufts Univ. (USA); Kishan Dholakia,

Univ. of St. Andrews (United Kingdom)

08.30: Nonlinear microscopy of tissues and embryo morphogenesis (Presentation Only), Emmanuel Beaurepaire, Ecole Polytechnique (France) . . . . . . . . . . . . [8090-45]Nonlinear microscopy is attractive for live tissue imaging. Second- and third-harmonic generation (SHG, THG) signals can provide structural information from unstained tissues, in conjunction with fluorescence. We will discuss recent applications to zebrafish embryogenesis and human cornea imaging, and ongoing developments such as pulse shaping and wavefront control.

09.00: Silk - new opportunities in optics and photonics for an ancient material (Presentation Only), Fiorenzo G. Omenetto, Tufts Univ. (USA) . . . . . . . . . . . . . . [8090-46]Famous for its use in clothing since early times, silk is now finding new applications as a useful biocompatible material platform with uti l ity in photonics and electronics. The talk will illustrate how purified silkworm silk can be reassembled in high quality, micro- and nanostructured optical and optoelectronic elements composed of the organic, biocompatible and implantable protein matrix.

09.30: Development of transient absorption ultrasonic microscopy, Brian E. Applegate, Ryan L. Shelton, Texas A&M Univ. (USA) . . . [8090-47]We have developed a novel hybrid i m a g i n g m o d a l i t y , T r a n s i e n t Absorption Ultrasonic Microscopy, that integrates nonlinear absorption with photoacoustic detection to achieve high-resolution absorption imaging. A prototype TAUM system was built, and used to image several capillaries in an excised hamster cheek pouch. Basic principles and recent progress will be discussed.

Conference End.

09.30: Design of a broadband near infrared spectroscopy (NIRS) and diffuse correlation spectroscopy (DCS) device with a self-calibrated probe for experimental oncology, Parisa Farzam, Turgut Durduran, ICFO - Instituto de Ciencias Fotónicas (Spain) [8088-41]We present the design of a self-calibrated probe based on numerical modeling for combined broadband near-infrared and diffuse correlation spectroscopy instrumentation for use on experimental, murine models in oncology.


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SESSION 1

Room: 5 Tues. 10.30 to 11.30

Advanced Coherent Sensing and Imaging

Concepts ISession Chair: Rainer A. Leitgeb, Medizinische Univ. Wien (Austria)

SESSION 1 continued09.45: The multifunctional application of microfluidic lab-on-a-chip surface-enhanced Raman spectroscopy (LOC-SERS) within the field of bioanalytics, Anne März, Bettina Mönch, Angela Walter, Thomas Bocklitz, Wilm Schumacher, Petra Rösch, Michael Kiehntopf, Friedrich-Schiller-Univ. Jena (Germany); Jürgen Popp, Institut für Photonische Technologien e.V. (Germany) [8087-04]This contribution will present a variety of applications of lab-on-a-chip surface enhanced Raman spectroscopy in the field of bioanalytic. Beside the quantification and online monitoring of drugs and pharmaceut ica ls , determination of enzyme activity and discrimination of bacteria are successfully carried out utilizing LOC-SERS.

Coffee Break . . . . . . . . .10.00 to 10.30

SESSION 2

Room: B13 Tues. 10.30 to 11.15

Minimally Invasive Diagnostics/Laboratory

Medicine IISession Chair: Stephen Allen

Boppart, Univ. of Illinois at Urbana-Champaign (USA)

10.30: Bacterial identification in real samples by means of micro-Raman spectroscopy (Invited Paper), Petra Rösch, Stephan Stöckel, Susann Meisel, Anja Bossecker, Ute Münchberg, Sandra Kloss, Wilm Schumacher, Jürgen Popp, Friedrich-Schiller-Univ. Jena (Germany) [8087-05]Cu l tu re independent pathogen identification is especially necessary for severe diseases like sepsis. Since Raman spectroscopy enables to identify single bacterial cells this method can be used for diagnosis without significant time delay.

SESSION 8 continued09.45: Design and evaluation of a modular, digital, frequency domain diffuse optical monitor, Nestor H. Oliverio, Jose C. Cifuentes, Parisa Farzam, Ricardo Saiz, Daniel Mitrani, Jordi Ninou, Oscar Casellas, Turgut Durduran, ICFO - Instituto de Ciencias Fotónicas (Spain) . . . . . . . . . [8088-42]A digital, frequency domain diffuse optical monitor (FD-DOM) is designed and evaluated. The new design utilizes the latest advances in digital signal processing in an undersampled, heterodyne approach to enable a modular, flexible instrument without the limitations of analog hardware.

Coffee Break . . . . . . . . .10.00 to 10.30

SESSION 9


Experimental MethodsSession Chairs: Xavier Intes,

Rensselaer Polytechnic Institute (USA); Paola Taroni, Politecnico

di Milano (Italy)




Imaging III



10.30: Time-domain diffuse optical spectroscopy beyond 1100 nm: initial feasibility study, Antonio Pifferi, Politecnico di Milano (Italy) and Consiglio Nazionale delle Ricerche (Italy); Ilaria Bargigia, Politecnico di Milano (Italy); Paola Taroni, Politecnico di Milano (Italy) and Consiglio Nazionale delle Ricerche (Italy); Andrea Farina, Politecnico di Milano (Italy); Rinaldo Cubeddu, Politecnico di Milano (Italy) and Consiglio Nazionale delle Ricerche (Italy) . . . . . . . . . . . . . . . . . . . [8088-43]We propose a system for t ime-domain diffuse optical spectroscopy extending up to 1300 nm. Preliminary measurements on Intralipid solution, collagen powder and the female breast in vivo are presented.

10.45: SNR enhancement by using polarized light for cortex functional imaging, Anabela da Silva, Institut Fresnel (France); Pierre Stahl, Ivo Vanzetta, Institut de Neurosciences Cognitives de la Méditerranée (France) . . . . . . . . . . . . . . . . . [8088-44]The method proposed here allows to perform a selection of a well defined subsurface volume in a turbid medium allowing SNR enhancement for functional imaging of the cortex. The method and preliminary results are presented.

10.30: High-speed functional OCT with self-reconstructive Bessel illumination at 1300nm (Invited Paper), Cedric Blatter, Branislav Grajciar, Medizinische Univ. Wien (Austria); Robert Huber, Ludwig-Maximilians-Univ. München (Germany); Rainer Leitgeb, Medizinische Univ. Wien (Austria) . . . . . . . . . . . . [8091-01]We present a Bessel beam illumination FDOCT setup with a buffered FDML swept source at 1300nm allowing for 220kHz A-scan rate. We applied the system for high-speed skin in-vivo imaging, profiting from the extended focus depth of the Bessel beam, as well as its self-reconstruction property. Furthermore, functional imaging of skin capillaries is shown.



SESSION 2 continued11.00: Optical parametric oscillator-based real-time breath monitoring by off-axis integrated cavity output spectroscopy, Denis D. Arslanov, Simona M. Cristescu, Frans J. M. Harren, Radboud Univ. Nijmegen (Netherlands) . . . . . . . . . . . . . [8087-06]An Optical Parametric Oscillator is combined with Off-Axis Integrated Cavity Output Spectroscopy for sensitive detection of methane (CH4), ethane (C2H6), water, carbon dioxide and acetone (C3H6O) in exhaled human breath at subsecond time scale.

SESSION 3

Room: B13 Tues. 11.15 to 12.30

Biospectroscopy and POC Diagnostics I

Session Chair: Stephen Allen Boppart, Univ. of Illinois at Urbana-Champaign (USA)

11.15: Fluorescence spectroscopy and cryoimaging of lung tissue mitochondrial redox state, Reyhaneh Sepehr, Univ. of Wisconsin-Milwaukee (USA); Said Audi, Marquette Univ. (USA); Kevin Staniszewski, Mahsa Ranji, Univ. of Wisconsin-Milwaukee (USA) . . . . . . . . . . . . . . . . . . . [8087-07]Fluorescence spectroscopy and imaging are used to assess mitochondrial intrinsic fluorophores as tissue metabolic markers. Mitochondrial respiratory chain inhibitors, KCN and DNP were studied on rats’ lung mitochondrial redox state.

SESSION 9 continued11.00: Time-resolved diffuse reflectance measurements at large source-detector separation: phantom experiments with dynamic inflow of ICG, Daniel Milej, Adam Liebert, Anna Gerega, Piotr Sawosz, Michal Kacprzak, Norbert S. Zolek, Roman Maniewski, Institute of Biocybernetics and Biomedical Engineering (Poland) . . . . . . . . . . . . . . . . . [8088-45]Time-resolved measurements of diffuse reflectance were carried out in phantom with dynamic inflow of indocyanine green at different depths and different source-detector separation. Preliminary results show high sensitivity of reflectance signals to the deeper inflow of the dye for large source-detector separation.

SESSION 1 continued11.00: Ex vivo tumour study using multispectral OCT, Aneesh Alex, Medizinische Univ. Wien (Austria); Catherine Kendall, Cranfield Univ. (United Kingdom); Bob Bonwick, Gloucestershire Royal Hospital (United Kingdom); Boris Pova?ay, Bernd Hofer, Medizinische Univ. Wien (Austria); Nicholas Stone, Cranfield Univ. (United Kingdom); Wolfgang Drexler, Medizinische Univ. Wien (Austria) . . . . . . . . . . . . . . . . . [8091-02]Optical coherence tomography is a non-invasive, real-time biomedical imaging modality capable of providing three dimensional micro-structural information of biological tissues. Ex vivo imaging of esophageal and colon tumor samples using OCT has been demonstrated at 800nm, 1060nm and 1300nm to investigate the optimum wavelength region for tumor imaging.




Imaging III



11.15: Comparison of polarized light depth penetration in scattering media, Simon Rehn, Anne Planat-Chrétien, Michel Berger, Jean-Marc Dinten, Commissariat à l’Énergie Atomique (France); Carole Deumié, Anabela da Silva, Institut Fresnel (France) . . . . . . . . . . . . . . . . . [8088-46]Monte Carlo simulations are used to investigate the scattering of differently polarized light in a semi-infinite scattering medium. The volumes probed by linearly, circularly and diverse elliptically polarized illuminations are compared. It is shown that with elliptically polarized light the depth sensitivity can be continuously selected.

11.15: Path-length-resolved forced-diffusive particle dynamics in Fourier-domain optical coherence tomography, Jeroen Kalkman, Academisch Medisch Ctr. (Netherlands); Rudolf Sprik, Univ. van Amsterdam (Netherlands); Yvo Mudde, Ton G. van Leeuwen, Academisch Medisch Ctr. (Netherlands) . . [8091-03]We describe a new method to measure the diffusion coefficient quantitatively and at high speed in a sample with a complex geometry with Fourier-domain optical coherence tomography. Our method is 200 times faster than conventional methods. The method is extended to forced-diffusive systems, i.e. systems with flow and diffusion

11.30: Analysis of the differences in diffuse reflectance spectra of skin due to detection system, Shoji Takano, Eiji Okada, Keio Univ. (Japan) . . . . . . . . . . . . . . . . . . [8088-47]The diffuse reflectance spectra of skin measured with a fibre probe and an integrating sphere is simulated by a multi-layered model. The differences in the spectra are analysed by the model in term of the wavelength dependence of the penetration depth of the detected light.

11.30: DNA sensing with optimized DVD substrates, Burak Turker, Bilkent Univ. (Turkey); Nihan Guvener, Hacettepe Univ. (Turkey); Hasan Guner, Sencer Ayas, Okan O. Ekiz, Handan Acar, Mustafa O. Guler, Bilkent Univ. (Turkey); Erhan Piskin, Hacettepe Univ. (Turkey); Aykutlu Dana, Bilkent Univ. (Turkey) . . . . . . . . . . . . . . . . . [8087-08]In this work we transform a Digital Versatile Disk (DVD) into a biomolecular affinity sensor for the label free detection of DNA interaction. Detection scheme is based on the excitation of surface plasmons via grating coupling.

11.45: Non-invasive SFG spectroscopy: a tool to reveal the conformational change of grafted chains due to bacterial adhesion, Emilie Bulard, Henri Dubost, Wanquan Zheng, Marie-Pierre Fontaine-Aupart, Univ. Paris-Sud 11 (France); Jean-Marie Herry, Marie-Noëlle Bellon-Fontaine, Romain Briandet, INRA-AgroParisTech (France); Bernard Bourguignon, Univ. Paris-Sud 11 (France) . . . . . . . [8087-09]Understanding the mechanisms of bacterial adhesion on a surface is a crucial step to design new materials. The present study demonstrates that bacterial adhesion can alter the conformation of grafted “brush” chains on Self-Assembled Monolayers (SAM) probed by the non invasive vibrational Sum Frequency Generation (SFG) spectroscopy.

11.45: Evaluation of the position resolution of NIR topography by localised visual stimulation, Hirokazu Kakuta, Eiji Okada, Keio Univ. (Japan) . . . . . . . . . . . . . . . . . . [8088-48]We demonstrate the improvement of the position resolution of near-infrared topography by double-density arrangement. The brain activation is evoked by visual stimuli and topographic image measured by the double-density arrangement is compared with that by the single-density arrangement. The double-density arrangement effectively improves the position resolution of near-infrared topography.

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SESSION 9 continued12.00: Simple method of improving sensitivity for diffuse reflective optical tomography: simulation and a phantom study, Keiko Fukuda, Kazuaki Koishi, Takanobu Murayama, Tokyo Metropolitan Institute of Technology (Japan) . . . . . . . . [8088-49]We proposed a convenient method for cancelling the skin blood volume change in the near-surface region by using two kinds of cancellation signals for fNIRS and simulation and phantom experiments show the effectiveness of our proposed method.

SESSION 3 continued12.00: Design and process development of a photonic crystal polymer biosensor for point-of-care diagnostic, Holger Egger, Bayer Technology Services GmbH (Germany); Kai Kolari, Tomi Haatainen, VTT Technical Research Ctr. of Finland (Finland); Peter Furjes, Research Institute for Technical Physics and Materials Science (Hungary); Damien Bernier, Multitel A.S.B.L. (Belgium); Basudev Lahiri, Univ. of Glasgow (United Kingdom); Fabian Dortu, Multitel A.S.B.L. (Belgium); Sandor Kurunczi, Research Institute for Technical Physics and Materials Science (Hungary); Jean-Charles Sanchez, Natacha Turck, Univ. of Geneva (Switzerland); Robert Horvath, Research Institute for Technical Physics and Materials Science (Hungary); Stefanie Eiden, Bayer Technology Services GmbH (Germany); Timo Aalto, VTT Technical Research Ctr. of Finland (Finland); Sam Watts, Stratophase Ltd. (United Kingdom); Peter Petrik, Research Institute for Technical Physics and Materials Science (Hungary); Nigel P. Johnson, Richard M. De La Rue, Univ. of Glasgow (United Kingdom); Domenico Giannone, Multitel A.S.B.L. (Belgium) . . . . . . . . . . . . . . . . [8087-10]We report the advances in the fabrication and anticipated performance of a polymer biosensor photonic chip. The photonic chip is fabricated from nano-composite polymeric materials, using highly scalable nano-imprint-lithography (NIL). A microfluidic structure for transporting the analyte solution is also fabricated in polymer and bonded to the photonic chip.

12.15: Effect of magnetic field in malaria diagnosis using magnetic nanoparticles, Quan Liu, Clement Yuen, Nanyang Technological Univ. (Singapore) . . . . . . . . . . . . . . [8087-11]Raman scattering from a metabolic byproduct of the malaria parasite (hemozoin) shows the possibil ity of rapid and objective diagnosis of malaria. We propose the sensitive detection of enriched β-hematin, whose spectroscopic properties are equivalent to hemozoin, based on surface enhanced Raman spectroscopy (SERS) by using magnetic nanoparticles.

Room: 5 Tues. 11.30 to 12.30

Poster PreviewsThe following authors will give

3-minute overviews of their posters. These posters will also be included in

the poster session.

Session Chair: Rainer A. Leitgeb, Medizinische Univ. Wien (Austria)

Towards multi-megahertz retinal OCT: wavelength swept 1050nm FDML laser at up to 3.1MHz repetition rate, Thomas Klein, Wolfgang Wieser, Christoph M. Eigenwillig, Benjamin R. Biedermann, Robert A. Huber, Ludwig-Maximilians-Univ. München (Germany) . . [8091 55] Axial resolution improvement by spectral data fusion in simultaneous dual-band optical coherence tomography, Peter Cimalla, Maria Gaertner, Julia Walther, Edmund Koch, Universitätsklinikum Carl Gustav Carus Dresden (Germany) . . . . . . . [8091 56] Functional OCT imaging of inherited retinal disease, Thomas Theelen, Carel B. Hoyng, B. Jeroen Klevering, Radboud Univ. Nijmegen (Netherlands); Barry Cense, Utsunomiya Univ.

Room: 5 . . . . . . . . . . . . . . . . . . . . . . .Tues. 14.00 to 15.30

ECBO Plenary SessionSession Chairs: Peter E. Andersen, Technical Univ. of Denmark (Denmark);

Irene Georgakoudi, Tufts Univ. (USA)

Optical spectroscopy for clinical detection of pancreatic cancer, Mary-Ann Mycek, Univ. of Michigan (USA) [8087-502]Twenty years of optical coherence tomography: where is it heading?, Wolfgang Drexler, Medizinische Univ. Wien (Austria) [8087-501]




Imaging III



12.15: Phantom experiments for quantitative evaluation of topographic image by mapping algorithm, Yusuke Yoshida, Hirokazu Kakuta, Keio Univ. (Japan); Hiroshi Kawaguchi, National Institute of Radiological Sciences (Japan); Eiji Okada, Keio Univ. (Japan) . . [8088-50]The optimal probe arrangements for mapping method were experimentally evaluated in terms of error in the topographic images. In the case of double-density arrangement, the error in position was less than 1.2mm and the broadening of the image is less dependence on the position of the absorption change.




(Japan) . . . . . . . . . . . . . . . . . [8091 57]


Conference 8087Clinical and Biomedical


Session Chairs: Jürgen Popp, Institut für Photonische Technologien e.V. (Germany);

Nirmala Ramanujam, Duke Univ. (USA)

Biospectroscopy and POC Diagnostics

Monitoring of head and neck tumor physiology in response to hyperoxia using a side-firing fiber optic probe, Bing Yu, Duke Univ. (USA); Gregory M. Palmer, Duke Univ. School of Medicine (USA); Bingqing Wang, Amy Shah, Karthik Vishwanath, Nirmala Ramanujam, Duke Univ. (USA) . . . . . . . . . . . . . . . . . . . [8087-60]This presentation reports the use of frequency-domain photon migration and a side-firing fiber optic probe for longitudinal monitoring of tumor physiology change upon breathing hyperoxic gas in a preclinical model with an application for head and neck cancer diagnosis and treatment monitoring.

A UV-Vis-NIR Fourier transform hyperspectral imaging platform for high-throughput multiplex biomolecular assay applications, Jianping Li, Robert K. Y. Chan, Hong Kong Baptist Univ. (Hong Kong, China) . . . . . . . . . . . . . . . . . . [8087-61]A hyperspectral imaging system covering 350-1000nm spectral range is realized by a UV-Vis-NIR Fourier transform imaging spectrometer with tunable spectral resolution up to ~10cm-1. Accurate and fast microspectroscopic measurement results on novel colloidal crystal microbeads demonstrate its practical potential for suspension array-based high-throughput multiplexed molecular assays.

Automatic cytometric device using multiple wavelength excitations, Nelly Rongeat, Sylvain Ledroit, Didier Cremien, Alexandra Urankar, Laurence Chauvet, HORIBA ABX (France); Vincent Couderc, Univ. de Limoges (France); Philippe Nerin, HORIBA ABX (France) . . . . . . . . . . . . . . . . . [8087-62]The apparatus combines techniques of hematology analysis and flow cytometry based on mult ip le f luorescence detection. This combined analyzer uses multiple wavelength excitations to improve the signal to noise ratio and to decrease spectral overlaps.

Testing of possibilities for establishing of nanodiamond-aptamer-conjugates, Andrea Pohl, Jan Michael, Jürgen Schreiber, Fraunhofer-Institut für Zerstörungsfreie Prüfverfahren (Germany); Elke Boschke, Philipp Weber, Technische Univ. Dresden (Germany); Viktoria A. Lapina, B.I. Stepanov Institute of Physics (Belarus); Jörg L. Opitz, Fraunhofer-Institut für Zerstörungsfreie Prüfverfahren (Germany) . . . . [8087-63]

We modified nanodiamonds (ND) produced by detonation synthesis and applied a two-step biofunctionalization of ND: upon chemical modification the biomolecules (aptamers) are bound. Numerous options of chemical surface modification of ND are being tested to find the most appropriate for binding aptamers and the formation of stable conjugates.

Microfluidic-CARS: a model system for studying isolated biological systems, Gero Bergner, Institut für Photonische Technologien e.V. (Germany); Benjamin Dietzek, Friedrich-Schiller-Univ. Jena (Germany); Thomas Henkel, Hartmut Bartelt, Institut für Photonische Technologien e.V. (Germany); Sebastian Schlücker, Univ. Osnabrück (Germany); Jürgen Popp, Institut für Photonische Technologien e.V. (Germany). . . . . . . . . . . . [8087-64]The combination of linear as well as nonlinear Raman microspectroscopy has been established as a powerful tool for potential biomedical diagnostics. In this contribution we discuss our recent approaches towards CARS based quantification of analytes.

Sepsis analysis with the channel array interrogation (CAI) instrument, Francesco Baldini, Ambra Giannetti, Cosimo Trono, Istituto di Fisica Applicata Nello Carrara (Italy); Luca Bolzoni, Giampiero Porro, Datamed S.r.L. (Italy) . . . . . . . . . . . . . . [8087-65]A Channel Array Interrogation (CAI) instrument for multiparameter analysis has been developed. In particular, the portable device was optimized for the C-reactive protein (CRP) and the procalcitonin (PCT), two of the analytes of interest for sepsis

Spectroscopic-tomography of biological membrane with high-spatial resolution by the imaging-type 2D Fourier spectroscopy, Asuka Inui, Ichiro Ishimaru, Kagawa Univ. (Japan) . . . . . . . . . . . . . . . . . . [8087-66]We proposed the imaging-type 2-D Fourier spectroscopy that is the phase-shift interferometry between the objective lights. The proposed method can measure the 2D spectral image at the limited depth. And in the depth direction, we can get the spectral distribution only in the focal plane.

VIS and NIR spectroscopy for the separation of haemoglobin changes of skin and calf muscle induced by a nonivamide/nicoboxil cream, Jan Warnecke, RheinAhrCampus Remagen (Germany); Thomas Wendt, Deutsche Sporthochschule Köln (Germany); Matthias Schak, RheinAhrCampus Remagen (Germany); Thorsten Schiffer, Wilhelm Bloch, Deutsche Sporthochschule Köln (Germany); Matthias Kohl-Bareis, RheinAhrCampus Remagen (Germany) . . . . . . . [8087-67]Spectroscopy in the VIS and NIR was exploited to evaluate the effect of the topical agents nonivamide / nicoboxil on haemoglobin concentration and oxygenation with a discrimination of skin and muscle. We show that there is an increase in oxygenation in calf muscle with a slower kinetics compared to skin.

Design of a tissue oxygenation monitor and verification on human skin, Hongyuan Liu, Moor Instruments, Ltd. (United Kingdom); Matthias Kohl-Bareis, RheinAhrCampus Remagen (Germany); Xiabing Huang, Moor Instruments, Ltd. (United Kingdom) . . . . . . . . . . . . . . . . [8087-68]We report the design of a tissue oxygen and temperature monitor. The non-invasive, fibre based moorVMS-OXY monitors tissue haemoglobin (Hb) and oxygen saturation (SO2) and is based on white-light reflectance spectroscopy. The spectroscopic algorithm takes into account the tissue scattering and melanin absorption for the calculation of Hb and SO2 using visible light. We found experimentally that the influence of the source-detector separation on the Hb parameters is small. This finding is discussed by Monte Carlo simulations for the depth sensitivity profile. The influence of probe pressure and the skin pigmentation on the measurement parameters a re a lso assessed experimentally. The combination with laser-Doppler technique demonstrates the importance of a measurement of both the Hb and the blood flow parameters for a full description of tissue perfusion. This is discussed in experimental data on human skin during cuff occlusion and after hyperemisation by a pharmacological cream.

Novel assay for direct fluorescent imaging of sialidase activity, Andriy Tomin, Tanya Shkandina, Rostyslav O. Bilyy, Institute of Cell Biology (Ukraine) . . . . . . . . . . . . . . . . [8087-69]The method of visualisation of sialidase activity in the cellular compartments was developed. For this purpose, 4-methylumbelliferol derivates were utilised, since, during the enzymatic digestion, the activity-dependent amount of fluorescent substance was accumulated, and may be detected and estimated in real time using fluorescent imaging, without desrtoying of the cell membrane.

Spectral discrimination of serum from liver cancer and liver cirrhosis using Raman spectroscopy, Tianyue Yang, Qiubao Yang, Xiaozhou Li, Shenyang Ligong Univ. (China) . . . . . . . . . . . . . . . . . [8087-70]Raman spectroscopy of human serum was used for the discrimination between normal people, liver cirrhosis patients and liver cancer patients. Both linear discriminant analysis and artificial neural network were applied on the extracted principal components. An accuracy of 85.19 % and 80.25 were obtained for PCA-LDA and PCA-ANN respectively.

Analysis of serum LIF-Raman spectroscopy for the diagnosis of liver cancer and liver cirrhosis, Tianyue Yang, Xiaozhou Li, Shenyang Ligong Univ. (China) . . . . . . . [8087-71]In this paper, laser-induced fluorescence (LIF) and Raman spectra of human serum were measured simultaneously using our fluorescence-Raman spectroscopy system, then the spectra was analyzed the multivariate statistical methods of principle component analysis (PCA). Then linear discriminant analysis (LDA) was utilized to differentiate the loading score plot of different diseases as the diagnosing algorithm. Artificial neural network (ANN) was used for cross-validation. The diagnosis sensitivity and specificity by PCA-LDA are 88.00 % and 79.14 %, while that of the PCA-ANN are 89.29% and 94.74 %. It is can be seen that modern analyzing method is a useful tool for the analysis of serum spectra for diagnosing diseases.

Spectral analysis of saliva from lung cancer using surface-enhanced Raman spectroscopy (SERS), Xiaozhou Li, Tianyue Yang, Shenyang Ligong Univ. (China) . . . . . . . [8087-72]Surface enhanced Raman spectroscopy (SERS) of whole saliva were used to detect the differences between lung cancer patients (n=21) and the healthy controls (n=22). The Raman intensity of some peaks represented protein and nucleic acid of lung cancer is lower than that of normal people. The PCA-LDA method gave an accuracy of 86.05%,a sensitivity of 94.12% and a specificity of 80.77%.

Wearable reflection oximetry using a green LED exploiting passive spectral tuning, Pavel Zakharov, Mark S. Talary, Andreas Caduff, Solianis Monitoring AG (Switzerland) . [8087-74]The wavelength tuning of a green LED caused by self-heating on actuation has been used to perform diffuse-reflectance spectroscopic measurements of blood oxygenation. The wearable monitoring system has been realised to monitor the cutaneous blood perfusion and benchmarked with broadband reflection spectroscopy.

Real-time multispectral processing of biological objects images, Boris S. Gurevich, Scientific Instruments Co. (Russian Federation); Valentine V. Shapovalov, Sergey V. Andreyev, Saint Petersburg Electrotechnical Univ. (Russian Federation); Andrey V. Belyaev, Scientific Instruments Co. (Russian Federation); Ilya A. Kolesov, Vyacheslav N. Chelak, Saint Petersburg Electrotechnical Univ. (Russian Federation) . . . . . . . . . . . . . . [8087-75]Biomedical technology development requires a high rate processing of images obtained during biological objects observation. The presented method provides selection of spectral information in the studied images. The operation principle and some features of the presented acousto-optic device intended for real time multispectral image processing, are considered.

Posters - TuesdayRoom: ICM Foyer Ground Floor · Tues. 15.30 to 17.00

Presenters may put up their posters Tuesday morning before the lunch/poster viewing break. Poster authors must be at their posters during the poster session from 15.30 to 17.00, and should take down posters immediately following this session. Coffee will be provided.

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The influence of 3d3 type transition metals on light scattering properties of sulfur cycle bacteria Desulfuromonas acetoxidans, Oleksandr I. Bilyi, Oresta M. Vasyliv, Svitlana O. Hnatush, Vasyl B. Getman, Ivan Franko National Univ. of L’viv (Ukraine); Galyna I. Kotsyumbas, State Scientific-Research Control Institute of Veterinary Preparations and Fodder Additives (Ukraine) . . . . . . . . [8087-76]Concentration changes and relative con ten t o f the bac te r i a l ce l l s Desulfuromonas acetoxidans under the influence of 3d3 type transition metals have been observed. Correlation between changes of their light scattering properties and growth has been shown.

Image analysis of free diffusing nanoparticles in vitro, Thorsten Wagner, Sven O. Lüttmann, Dominic Swarat, Fachhochschule Dortmund (Germany); Martin Wiemann, IBE GmbH (Germany); Hans-Gerd Lipinski, Fachhochschule Dortmund (Germany) . . . . . . . . . . . . . . . [8087-77]It is possible to visualize nanoparticles as light scattering objects by means of a conventional light microscope. Image series of visualized nanoparticles were used to calculate characteristic parameters from dynamic properties and particle shape parameters using image analysis methods. Together these techniques may help to better define nanoparticles as used for cell culture experiments.

Detection and discrimination of normal and colorectal cancer serum using Raman spectroscopy, Xiaozhou Li, Tianyue Yang, Shenyang Ligong Univ. (China) . . . . . . . . . . . . . [8087-78]The Raman spectral differences between normal people, colon cancer patients and rectum cancer patients were investigated. The selected parameters represented the features of Raman and background fluorescence were input to the principal component regression (PCR) and partial least square regression (PLSR), and were classified by linear discriminant analysis (LDA). We got a sensitivity of 86.36 % and 82.61 % for rectum and colon cancer separately through PCR-LDA.

Feature extraction of serum LIF-Raman spectroscopy for the diagnosis of colorectal cancer, Xiaozhou Li, Sr., Shenyang Ligong Univ. (China); Tianyue Yang M.D., Shenyang Univ. of Technology (China) . . . . . . . . . . . . . . . . . . [8087-79]

The serum of normal people, colon cancer patients and rectum cancer patients were investigated using laser induced fluorescence (LIF) and Raman spectroscopy to f ind the diagnosis potential by the variation. The spectroscopy features of both the Raman peaks and the fluorescence background were used as the selected parameters for further analysis. Principal component regression (PCR) and partial least square regression (PLSR) were used on the LIF-Raman spectroscopy. PCR performs more effectively than PLSR in the components extraction in our data. Then, linear discriminant analysis (LDA) was used to differentiating different groups. The PCR-LDA resulted in a sensitivity of 80.85 % and a specificity of 86.36 % for rectum cancer, for colon cancer are 97.44 % and 82.61 %. Our statistical evaluation suggested that LIF-Raman spectroscopy of human serum may be a useful tool for the diagnosis of colorectal cancer.

Skin Diagnostics and Therapy

Validation of a 2D multispectral camera: application to dermatology/cosmetology on a population covering five skin phototypes, Romuald Jolivot, Univ. de Bourgogne (France); Hermawan Nugroho, Univ. Teknologi Petronas (Malaysia); Pierre Vabres, Univ. de Bourgogne (France); M. Hani Ahmad Fadzil, Univ. Teknologi Petronas (Malaysia); Franck Marzani, Univ. de Bourgogne (France) [8087-80]This paper presents the validation of a multispectral camera specifically developed for dermatological application based on healthy participants from five different skin phototypes. The study reveals that the multispectral camera provides accurate reconstruction of hyperspectral cube of cutaneous data which can be used for analysis of skin reflectance spectrum.

Using MAP to recover the optical properties of a biological tissue from reflectance measurements, Amaria Zidouk, Iain B. Styles, The Univ. of Birmingham (United Kingdom) [8087-81]We present a method based on the maximum a posteriori technique to recover the concentrations of melanin and haemoglobin present in a biological tissue from reflectance measurements. A preliminary analysis of the results suggests that the proposed method is robust against high levels of uncertainty in the tissue’s model.

RGB mapping of hemoglobin distribution in skin, Dainis Jakovels, Janis Spigulis, Laura Rogule, Univ. of Latvia (Latvia) . . . . . . . . . . . . [8087-82]An experimental RGB imaging system based on commercial color camera was constructed, and its potential for mapping of hemoglobin distribution in skin was studied. Two types of LEDs (RGB and white “warm” LEDs) were compared as illuminators for acquiring images of vascular and pigmented skin malformations.

Evaluation of a novel fiber optical probe for spatially and spectrally resolved reflectance (SRR) measurements of turbid media, Heiko Luckmann, Laser- und Medizin-Technologie GmbH, Berlin (Germany); Stefan Andree, Carina Reble, Laser- und Medizin-Technologie GmbH, Berlin (Germany) and Technische Univ. Berlin (Germany); Ingo Gersonde, Jürgen Helfmann, Laser- und Medizin-Technologie GmbH, Berlin (Germany) . . . . . . . . . . . . . . . [8087-83]A fiber applicator for spatially resolved reflectance measurements is presented, which uses simultaneous read-out of spectrometers for each source-detector separation. Different calibration procedures are compared by using silicone as well as liquid phantoms

Development of a fiber-based Raman probe for clinical diagnostics, Ines Latka, Sebastian Dochow, Christoph Krafft, Institut für Photonische Technologien e.V. (Germany); Benjamin Dietzek, Friedrich-Schiller-Univ. Jena (Germany); Hartmut Bartelt, Jürgen Popp, Institut für Photonische Technologien e.V. (Germany) [8087-84]Here we present our recent progress towards designing a fiber-based Raman probe, which - in perspective - might be incorporated into the working channel of a surgical endoscope. We will review the general design principle of such a device and the specific design strategy for our Raman probe. Furthermore, calibration experiments on human tissue sections are presented.

Multispectral photoplethysmography technique for parallel monitoring of pulse shapes at different tissue depths, Lasma Asare, Edgars Kviesis-Kipge, Uldis Rubins, Oskars Rubenis, Janis Spigulis, Univ. of Latvia (Latvia) . . . . . . . . . . . . . . . . . [8087-85]Multi-spectral photoplethysmography (MS-PPG) biosensor intended for analysis of peripheral blood volume pulsations at different vascular depths has been designed and experimentally tested. Multi-spectral monitoring was performed by means of a three-wavelengths (405 nm, 660 nm and 780 nm) laser diode and a single photodiode with multi-channel signal output processing. The pulse signals of volunteers were monitored during one week to follow the experimental data recurrence. The proposed methodology and potential clinical applications are discussed.

Photobleaching measurements of pigmented and vascular skin lesions: results of a clinical trial, Janis Lesins, Univ. of Latvia (Latvia) . . . . . . [8087-86]The autofluorescence photobleaching intensity dynamics of in vivo skin and skin pathologies under continuous 532 nm laser irradiation have been studied. Overall the 47 human skin malformations were investigated by laser induced skin autofluorescence photobleaching analysis. Details of equipment are described along with some measurement results illustrating potentiality of the technology.

Melanoma-nevus differentiation by multispectral imaging, Ilze Diebele, Ilona Kuzmina, Univ. of Latvia (Latvia); Janis Kapostinsh, Alexander Derjabo, SIA Riga Eastern Clinical Univ. Hospital (Latvia) . . . . . . . . . . . . . . . . . . [8087-87]A cl inical tr ial on mult i-spectral imaging studies of malignant and non-malignant skin pathologies comprising 16 melanomas and 25 pigmented nevi was performed in Latvian Oncology Center. Analysis of data obtained in the spectral range 450-950 nm have led to a novel image processing algorithm capable to distinguish melanoma from pigmented nevi.

Bimodal spectroscopy for in vivo characterization of hypertrophic skin tissue: pre-clinical experimentation, data selection and classification, Honghui Liu, CRAN INPL (France); Héloïse Gisquet, CHU Brabois Enfants (France); Walter C. P. M. Blondel, CRAN INPL (France); François H. Guillemin, Ctr. Alexis Vautrin (France) . . . . . . . . . . . . . . . . . [8087-88]The main objective of this study was to evaluate the effect of an antiinflammatory medication (tacrolimus) in inhibiting hypertrophic formation on scarring tissue by using our bimodal spectroscopic system.This study was conducted on 20 New Zealand Rabbits who had received hypertrophic scarring treatment on their ears. Fluorescence and Diffuse Reflectance spectra were collected from each scar, amongst which certain had received tacrolimus treatment. Spectral features were extracted from collected data and analyzed to classify the scarring tissues into hypertrophic or non-hypertrophic. Diagnostic algorithm was developed, in applying KNN classifier on a set of spectral features extracted with our innovative feature extraction-selection method. The efficiency of this algorithm was finally validated by comparing to histological classification with Leave-one- out cross validation method. The accuracy for detecting hypertrophy was good (sensibility : 94.44%,specificity : 90.48%).

Clinical and Preclinical Tissue Characterization

Model-based spectral analysis of photon propagation through nanoparticle-labeled epithelial tissues, Can Cihan, Bilkent Univ. (Turkey); Dizem Arifler, Eastern Mediterranean Univ. (Turkey) [8087-89]Metal nanoparticles can function as optical contrast enhancers for reflectance-based diagnosis of epithelial precancer. We perform Monte Carlo simulations to analyze the spectral reflectance response of nanoparticle-labeled tissues and to quantify the achievable contrast enhancement.

Spectral imaging as a potential tool for optical sentinel lymph node biopsies, Jack D. O’Sullivan, Paul R. Hoy, Harvey N. Rutt, Univ. of Southampton (United Kingdom) . . . . . . . . . . . . . . . . [8087-90]

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We investigate the potential use of spectral imaging for performing an optical sentinel lymph node biopsy. Dummy images generated f rom previously published data are analysed using a spectral angle map and we conclude that there is scope for spectral imaging to form the basis of an optical biopsy technique.

Preliminary results of normalized autofluorescence imaging diagnostics in upper GI, Alexandre Douplik, Friedrich-Alexander-Univ. Erlangen-Nürnberg (Germany) [8087-91]This paper presents an attempt transferring the autofluorescence spectroscopic findings into imaging mode to improve specificity of cancer diagnostics in upper gastrointestinal tract. The preliminary results show that after applying a new algorithm of image processing the specificity increased by 20%.

Method to determine optimal illumination wavelengths for gold nanoparticle detection in tissue using reflectance spectroscopy, Pilar Beatriz García-Allende, Clement Barriere, Vincent Sauvage, Daniel S. Elson, Imperial College London (United Kingdom) . . . . . . . . . . . . . . . . [8087-92]A multi-spectral illumination approach for real-time mapping of the presence of gold nanoparticles in bulk tissue is presented. A Principal Component Analysis method is fol lowed for determining the wavelengths that will make up a multispectral imaging endoscope.

A clinical Raman spectrometer for macroscopic mapping of cervical intraepithelial neoplasia, Carina Reble, Ingo Gersonde, Cathrin Dressler, Jürgen Helfmann, Daniela Schädel, Laser- und Medizin-Technologie GmbH, Berlin (Germany); Wolfgang Kühn, Günter Cichon, Achim Schneider, Charité Universitätsmedizin Berlin (Germany); Hans Joachim Eichler, Technische Univ. Berlin (Germany); Gerd Illing, Laser- und Medizin-Technologie GmbH, Berlin (Germany) . . . . . . . . . . . . . . . [8087-94]A clinical instrument was built for macroscopic Raman mapping of cervical tissue samples. Monte Carlo simulations were used to guide the choice of the measurement geometry in order to obtain a suitable sampling volume



Phantoms of normal and abnormal tissues for calibration of hyperspectral reflectance and multispectral fluorescence video endoscopy: toward gastrointestinal diagnostics, Martin Hohmann, Alexandre Douplik, Friedrich-Alexander-Univ. Erlangen-Nürnberg (Germany) and Erlangen Graduate School in Advanced Optical Technologies (Germany); Aulia Nasution, Institut Teknologi Sepuluh Nopember (Indonesia); Jonas Mudter, Markus Neurath, Friedrich-Alexander-Univ. Erlangen-Nürnberg (Germany); Michael Schmidt, Friedrich-Alexander-Univ. Erlangen-Nürnberg (Germany) and Erlangen Graduate School in Advanced Optical Technologies (Germany) . . . . . . . . . . . . . . . [8087-95]This paper presents the calibration and test results obtained by mean of a hyperspectral reflectance and multispectral fluorescence flexible video endoscope setup developed for gastrointestinal cancer detection. We fabricated hard tissue phantoms which mimic different types of tissue in terms of its reflection and fluorescence properties for evaluation.

Three years of treatment with once-yearly zoledronic acid influences the kinetics of bone matrix maturation in osteoporotic patients independent of bone turnover, Sonja Gamsjaeger, Birgit Buchinger, Ludwig Boltzmann Institut (Austria); Juerg A. Gasser, Novartis Institutes for Biomedical Research, Inc. (Switzerland); Eric F. Eriksen, AUS Aker Universitetssykehus Trondheimsveien (Norway); Eleftherios P. Paschalis, Klaus Klaushofer, Ludwig Boltzmann Institut (Austria) . . [8087-96]Biopsies of the HORIZON-PFT trial (Zoledronic Acid, yearly 5-mg infusions over a 3-yr period) were analyzed by Raman microspectroscopy as a function of tissue age (based on tetracycline fluorescent labels) to determine whether ZOL therapy has an effect on the kinetics of maturation of material properties.

In vivo determination of absorption and scattering properties in rat cerebral cortex using single reflectance fiber probe with two source-collector geometries, Izumi Nishidate, Tokyo Univ. of Agriculture and Technology (Japan); Satoko Kawauchi, Shunichi Sato, National Defense Medical College (Japan); Manabu Sato, Yamagata Univ. (Japan); Miya Ishihara, Makoto Kikuchi, National Defense Medical College (Japan) . . . . . . . . . . . . . . . . . . [8087-97]We present a new method determining the absorption coefficients and the reduced scattering coefficients of in vivo rat cerebral cortex using single reflectance fiber probe with two source-collector geometries.

The in vivo monitoring method for traumatic brain injury of mouse based on near-infrared light intensity, Weitao Li, Zhiyu Qian, Xuena Wang, Xing Liu, Nanjing Univ. of Aeronautics and Astronautics (China) . . . . [8087-98]A system based on near-infrared light intensity was proposed to monitoring traumatic brain injury (TBI) of mouse. The system can measure βHb and βHbO2. Then the mouse TBI models were used to prove that the proposed system can be used to monitoring TBI of mouse in vivo.

Post-DeadlineComplex degree of mutual anisotropy of biological liquid crystals net, Alexander V. Dubolazov, Yuriy Fedkovych Chernivtsi National Univ. (Ukraine) . . . . . . . . . . . . [8087-99]To characterize the degree of consistency of parameters of the optically uniaxial birefringent liquid crystals (protein fibrils) nets of biological tissues (BT) a new parameter - complex degree of mutual anisotropy is suggested. The technique of polarization measuring the coordinate distributions of the complex degree of mutual anisotropy of biological tissues is developed. It is shown that statistic approach to the analysis of complex degree of mutual anisotropy distributions of biological tissues of various morphological and physiological states and optical thicknesses appears to be more sensitive and efficient in differentiation of physiological state in comparison with investigations of complex degree of mutual polarization of the corresponding laser images.

Optical fiber probe for infrared food safety and medical diagnosis, Marie-Laure Anne, Bruno Bureau, Olivier Loreal, Catherine Boussard-Pledel, Valérie Monbet, Univ. de Rennes 1 (France) . . . . . . . . . . . . . . . .[8087-100]Fiber Evanescent Wave Spectroscopy (FEWS) coupled to PLS regression has proved to be a powerful technology for the study of biomedical samples. Progress in the design of chalcogenide fibers have enabled to obtain optical fiber with a head-probe which can be used for endoscopic measurements.

Imaging prosthetic implant related wear debris in macrophages using coherent anti-Stokes Raman scattering spectroscopy, Martin Lee, Alistair Elfick, University of Edinburgh (United Kingdom) . . . . . . . .[8087-101]The use of CARS as a method of detecting unlabeled material in the nanoparticle size range is assessed in both individual particles and those within macrophage cells. The results suggest that CARS is a potential tool for monitoring the accumulation of wear debris generated by prosthetic implants.

Contactless cardiac pulse measurements during exercise using imaging PPG with motion artifacts reduction, Yu Sun, Sijung Hu, Vincete Azorin-Peris, Loughborough Univ. (United Kingdom); Stephen Greenwald, Barts & The London School of Medicine and Dentistry (United Kingdom); Jonathon Chambers, Loughborough Univ. (United Kingdom); Yisheng Zhu, Shanghai Jiao Tong University (China) . . . . . . . . . . . . . . . . .[8087-102]

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The motion artifact reveals major drawback in the present iPPG in particular clinical assessment. In this paper, selected motion artifacts reduction techniques were used in the experimental iPPG system to obtain continuous monitoring of the respiratory and pulsatile variation during cycling exercise. To evaluate the reliability and sensitivity of the iPPG system and also motion componsation techniques, e.g., two levels of the physical exercise were introduced. In this study 10 volunteers were involved in a 12 minute continuous recording. The t ime-f requency-representation (TFR) method was used to visualize the time-dependent behavior of the signal frequency. The results show that heart and respiration rates could be successfully traced even under a high-intensity physical exercise.

Modeling and measuring extravascular hemoglobin: aging contusions, Collin Lines, Oleg Kim, Univ. of Notre Dame (United States); Susan Duffy, Rhode Island Hospital (United States); Mark Alber, Gregory Crawford, Univ. of Notre Dame (United States) . . . . . . . . . . . . . . . . .[8087-103]A review of current spectroscopy based techniques and model ing methods assisting in retrieval of the age of extravascular contusions, and presents a new study with reflectance spectrophotometric data obtained using an Ocean Optics HR4000 spectrometer compared with a novel Monte Carlo technique for 3D photon tracking and blood transport model.

Towards a realistic numerical modelling of the polarimetric response of healthy and pathological colon tissue, Maria Rosaria Antonelli, Tatiana Novikova, Angelo Pierangelo, Antonello De Martino, Ecole Polytechnique (France) . . . .[8087-104]We used the Monte-Carlo method to simulate the propagation of polarized light in a stack of scattering layers, as a first model of the polarimetric response of human colon tissue. Bi modal population of scatterers, including very small ones (50 nm) are needed for qualitative agreement with the experimental results.




of the peritoneal membrane in mice of different sizes and weights.As part of this preliminary study, healthy mice of different weights were euthanized and the thickness of the peritoneal membrane was measured using OCT. The aim was to gather data on the expected range of thicknesses present in healthy animals for future studies.

Blind deconvolution algorithm for restoration OCT images with diffraction limited resolution, Alexander A. Moiseev, Grigory V. Gelikonov, Pavel A. Shilyagin, Valentine M. Gelikonov, Institute of Applied Physics (Russian Federation) [8091-67]The problem of recovering diffraction limited resolution of OCT images from blurred one, obtained due to acquisition with sharp focused scanning beam, had been sold as a blind deconvolution problem. Blind deconvolution algorithm, suitable for recovering OCT images, had been developed.

Early characterization of occlusal overloaded cervical dental hard tissues by en face optical coherence tomography, Corina Marcauteanu, Meda Lavinia Negrutiu, Cosmin G. H. Sinescu, Eniko Tunde Stoica, Florin Ionel Topala, Liliana Vasile, Univ. de Medicina si Farmacie Victor Babes, Timisoara (Romania); Adrian Bradu, George M. Dobre, Adrian G. Podoleanu, Univ. of Kent (United Kingdom) . . . . . . . . . . . . . . . . [8091-68]Our s tudy p roposes the ea r l y microstructural characterization of occlusal overloaded bicuspids by en face optical coherence tomography (eFOCT). The eFOCT images obtained from the occlusal overloaded bicuspids, with a normal crown morphology, visualized cracks, which didn’t reach the tooth surface. The µCT and histological images confirmed the microstructural defects identified on eFOCT images.

Study on image feature extraction and classification for human colorectal cancer using optical coherence tomography, Shu-Wei Huang, Shan-Yi Yang, Wei-Cheng Huang, Industrial Technology Research Institute (Taiwan); Han-Mo Chiu, National Taiwan Univ. Hospital (Taiwan); Chih-Wei Lu, Industrial Technology Research Institute (Taiwan) . . . . . . . . . . . . . . . . . [8091-69]In this study, we investigated the relationship between the image feature of B-scan OCT and the slice obtained from human colorectal cancer biopsy, also en-face OCT image and the endoscopy image pattern. The OCT images correct classification of adenoma and SM massive and compared with the biopsy, the pit-pattern of en-face OCT has similar to endoscopy image.

1550 nm superluminescent diode and anti-Stokes effect CCD camera based optical coherence tomography for full-field optical metrology, Michael J. Connelly, Lukasz Kredzinski, Univ. of Limerick (Ireland) . . . [8091-70]We demonstrate a comparatively simple and inexpensive time-domain full-field OCT system, which uses a Michelson interferometer, a 1550 nm superluminescent diode, anti-stokes effect based CCD camera and simple signal processing to perform 3D cross-sectional imaging of a mirror stack with a depth resolution of 10 microns.

and in unchanged retinal sectors of patients with retinal dystrophies (RD). In affected areas of RD eyes IOS were significantly reduced or even absent.

Investigation of alveolar tissue deformations using OCT combined with fluorescence microscopy, Maria Gärtner, Peter Cimalla, Lilla Knels, Sven Meissner, Christian Schnabel, Universitätsklinikum Carl Gustav Carus Dresden (Germany); Wolfgang Kuebler, Charité Universitätsmedizin Berlin (Germany); Edmund Koch, Universitätsklinikum Carl Gustav Carus Dresden (Germany) . . . . . . . . [8091-58]A combined setup utilizing optical coherence tomography and confocal fluorescence microscopy is used to study the elastic behavior of the alveolar tissue in an in vivo mouse model post mortem under different CPAP values. Three-dimensional volume changes and distributions of elastic fibers are quantified and discussed.

Structural analysis of artificial skin equivalents, Robert Schmitt, RWTH Aachen (Germany) and Fraunhofer-Institut für Produktionstechnologie (Germany); Ulrich Marx, Fraunhofer-Institut für Produktionstechnologie (Germany); Heike Walles, Lena Schober, Fraunhofer-Institut für Grenzflächen- und Bioverfahrenstechnik (Germany) . . . . . . . . . . . . . . . [8091-59]We developed an automatic OCT Device to monitor Artificial Skin Equivalents ASEs. We used image processing algorithms to characterize the surface structure, which may function as an indicator for defects in the epidermal stratum corneum. Further, we analysed the tomographic morphologica l structure of the ASEs to describe the aging process.

Multilayer tissue phantoms with embedded capillary system for OCT and DOCT imaging, Alexander V. Bykov, Alexey P. Popov, Univ. of Oulu (Finland); Alexander V. Priezzhev, Lomonosov Moscow State Univ. (Russian Federation); Risto Myllyla, Univ. of Oulu (Finland) . . . . . . [8091-60]We report about manufacturing of fully functional capil lary network embedded into the multilayer tissue phantom. Polyvinyl chloride-plastisol was used as a host transparent medium. Scattering was introduced by adding the TiO2 submicron particles. OCT and Doppler OCT techniques were used to characterize the manufactured phantoms and to monitor the flow through the vessels.

Evaluation of a swept-laser OCT light source based on a novel quantum-dot based SOA, Nik Krstajic, Deepa Kasaragod, Stephen J. Matcher, David T. D. Childs, The Univ. of Sheffield (United Kingdom); Igor L. Krestnikov, Innolume GmbH (Germany); Richard A. Hogg, The Univ. of Sheffield (United Kingdom) . . . . . . . . . . . . . . . . [8091-61]We describe a swept-laser that uses a novel quantum-dot SOA. The laser operates at 1220 nm centre wavelength with a 60 nm bandwidth and a peak power output of 12 mW. An evaluation laser operating at a low sweep rate demonstrates a 10 mm coherence length. SS-OCT images for in-vivo skin and other tissues are presented.

Imagistic evaluation of direct dental restoration: en face OCT versus SEM and microCT, Meda-Lavinia Negrutiu, Cosmin Sinescu, Florin Ionel Topala, Univ. de Medicina si Farmacie Victor Babes, Timisoara (Romania); Ciprian Ionita, Toshiba Stroke Research Ctr. (USA); Corina Marcauteanu, Emanuela L. Petrescu, Univ. de Medicina si Farmacie Victor Babes, Timisoara (Romania); Adrian G. Podoleanu, Univ. of Kent (United Kingdom) . . . [8091-62]The purpose of this in vitro study was to validate the en face OCT imagistic evaluation of direct dental restoration by using SEM and microCT. Teeth after several treatment methods are imaged in order to detect material defects and to asses the marginal adaptation at the dental hard tissue walls.

Optical coherence tomography study of mechanical deformation of skin, Pavel D. Agrba, Vladislav A. Kamensky, Mikhail Y. Kirillin, Institute of Applied Physics (Russian Federation); Ekaterina A. Bakshaeva, N.I. Lobachevsky State Univ. of Nizhni Novgorod (Russian Federation) . . . . . . . . . . . . . . [8091-64]We study mechanical compression and transversal deformation of human thin skin using optical coherence tomography. The study is performed in vivo on three groups of volunteers of different age with an OCT system equipped with a contact en-face probe.

Spectroscopic optical coherence tomography for substance identification, Volker Jaedicke, Ruhr-Univ. Bochum (Germany); Helge Wiethoff, Technische Fachhochschule Georg Agricola zu Bochum (Germany); Christoph Kasseck, GSKH Essen (Germany); Nils C. Gerhardt, Ruhr-Univ. Bochum (Germany); Hubert Welp, Technische Fachhochschule Georg Agricola zu Bochum (Germany); Martin R. Hofmann, Ruhr-Univ. Bochum (Germany) . . . . . . . . . . . . . . . [8091-65]Spectroscopic Optical Coherence Tomography y i e lds add i t i ona l information in comparison to pure backscattering analysis. In our proof of principle we demonstrated the reconstruction of spectral transfer functions with high accuracy using a spectral calibration technique. Since calibration is not feasible in most cases we also demonstrated substance identification using pattern recognition.

Measuring the thickness of the peritoneal membrane in mice using optical coherence tomography, Reem O. Al-Wafi, Mark R. Dickinson, The Univ. of Manchester (United Kingdom); Paul Brenchley, Manchester Royal Infirmary (United Kingdom); Louise Walkin, The Univ. of Manchester (United Kingdom) . . . . . . . . . [8091-66]In recent years, the detection and diagnosis techniques of diseases have improved. One such technique is optical coherence tomography (OCT), which is used in many medical applications to perform internal microstructural imaging of the human body at high resolution. OCT is non-invasive and can be used as a contact or non-contact technique to obtain an image. In medicine, there are many applications that involve OCT, such as in ophthalmology and oncology. This work demonstrates the use of an OCT system incorporating a swept laser with a high sweep rate of 16 kHz over a wide range of wavelengths (1260nm to 1390nm) to measure the thickness

Poster Previews continued

Spectroscopic optical coherence tomography for substance identification, Volker Jaedicke, Ruhr-Univ. Bochum (Germany); Helge Wiethoff, Technische Fachhochschule Georg Agricola zu Bochum (Germany); Christoph Kasseck, GSKH Essen (Germany); Nils C. Gerhardt, Ruhr-Univ. Bochum (Germany); Hubert Welp, Technische Fachhochschule Georg Agricola zu Bochum (Germany); Martin R. Hofmann, Ruhr-Univ. Bochum (Germany) . . . . . . . . . . . . . . [8091 65] Measuring the thickness of the peritoneal membrane in mice using optical coherence tomography, Reem O. Al-Wafi, The Univ. of Manchester (United Kingdom) . . . . . . . . [8091 66] Integration of spectral domain optical coherence tomography with microperimetry generates unique datasets for the simultaneous identification of visual function and retinal structure in ophthalmological applications, Peter Koulen, Gary S. Gallimore, Ryan D. Vincent, Nelson R. Sabates, Felix N. Sabates, UMKC School of Medicine (United States) . . . . . . . . . . . . . . . . . [8091 71] Lunch and poster viewing . . . . . . . . . . . . .12.30 to 14.00

Conference 8091Optical Coherence Tomography and

Coherence Techniques VSession Chair: Rainer A. Leitgeb, Medizinische Univ. Wien (Austria)

Towards multi-megahertz retinal OCT: wavelength swept 1050nm FDML laser at up to 3.1MHz repetition rate, Thomas Klein, Wolfgang Wieser, Christoph M. Eigenwillig, Benjamin R. Biedermann, Robert A. Huber, Ludwig-Maximilians-Univ. München (Germany) . . [8091-55]We present a novel multi-megahertz Fourier-domain mode locked laser operating around 1050nm. Record sweep rates of up to 3.14MHz are achieved, and performance for swept-source retinal optical coherence tomography will be discussed.

Axial resolution improvement by spectral data fusion in simultaneous dual-band optical coherence tomography, Peter Cimalla, Maria Gaertner, Julia Walther, Edmund Koch, Universitätsklinikum Carl Gustav Carus Dresden (Germany) . . . . . . . . [8091-56]A method fo r ax ia l r eso lu t ion improvement by adequate spectral data fusion of two parallel acquired disjunct wavelength bands centered at 800 nm and 1250 nm is demonstrated in the field of high resolution simultaneous dual-band optical coherence tomography in the spectral domain using one low-coherent supercontinuum laser source.

Functional OCT imaging of inherited retinal disease, Thomas Theelen, Carel B. Hoyng, B. Jeroen Klevering, Radboud Univ. Nijmegen (Netherlands); Barry Cense, Utsunomiya Univ. (Japan) . . . . . . . . . . . . . . . . . . [8091-57]Intrinsic optical signals (IOS) could be measured by a commercially available spectral-domain OCT in healthy retinas

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Integration of spectral domain optical coherence tomography with microperimetry generates unique datasets for the simultaneous identification of visual function and retinal structure in ophthalmological applications, Peter Koulen, Gary S. Gallimore, Ryan D. Vincent, Nelson R. Sabates, Felix N. Sabates, UMKC School of Medicine (USA) . . . [8091-71]Standard automated perimetry proves difficult for retina and macular disease due to the need for central and steady fixation. Microperimetry incorporates eye tracking for placement of macular sensitivity values onto structural maps acquired with spectral domain optical coherence tomography generating a precise functional and anatomical mapping of the visual field.

Design and development of a dual beam Fd-OCT system for volumetric vascular perfusion imaging of the human skin, Susan M. Daly, Enock Jonathan, Martin J. Leahy, Univ. of Limerick (Ireland) . . . . . . . . . . [8091-72]Fourier domain OCT systems have been reported to operate with higher imaging speeds and exhibit superior sensitivities when compared with Time domain OCT systems. However, these high acquisition rates were inevitably of low sensitivity to flow rate. In order to enhance sensitivity, multiple scans taken of the same sample position or acquisition speeds decreased. In addition, many techniques commonly employ point scanning, thus are not optimised for time-evolution studies as signals are acquired at different times.

The proposed method operates by simultaneous il lumination and measurement from two planes in one acquisition. The temporal variations in light intensity are then to be subjected to real time cross-correlation to determine the transit time (∆t) of photometric events and the red blood cell velocity (RBCV) may be obtained from the ratio of δ/∆t.

Screening cervical and oesophageal tissues using optical coherence tomography, Gavin R. G. Erry, National Physical Lab. (United Kingdom); Florian Bazant-Hegemark, Michelson Diagnostics Ltd. (United Kingdom); Mike D. Read, Nicholas Stone, Gloucestershire Royal Hospital (United Kingdom) . . . . . . . . . . . . . . . . [8091-73]Opt ical Coherence Tomography can generate images of sub-surface structures of biological tissue. By using the scattering data from the OCT system and analysed by gradient and principal components analysis, data can be grouped into normal and various stages of cancer. Results from OCT data collected from cervical tissues is shown.

Digital in-line holographic microscopy of marine microorganisms in multi-media environment, Gonzalo H. Sendra, Sebastian Weisse, Ruprecht-Karls-Univ. Heidelberg (Germany); Maureen E. Callow, Jim A. Callow, The Univ. of Birmingham (United Kingdom); Michael Grunze, Axel Rosenhahn, Ruprecht-Karls-Univ. Heidelberg (Germany) . . . . . . . . . . . . . . . [8091-74]

Digital In-line Holographic Microscopy (DIHM) has shown to be a suitable technique for tracking motile marine microorganisms. The distortion of the wavefront when light go across media with different refractive indices is analyzed and compensated.

OCT in difficult diagnostic cases in gynecology, Grigory V. Gelikonov, Natalia M. Shakhova, Institute of Applied Physics (Russian Federation); Olga G. Panteleeva, Nizhny Novgorod Clinical Hospital of Russian Railways (Russian Federation); Ekaterina Yunusova, Nizhny Novgorod State Medical Academy (Russian Federation) . . . . . . . . . . . . . . [8091-75]Clinical application of OCT for diagnosing of pelvic inflammatory diseases (PID) and endometriosis is reported. High (75-85%) diagnostic accuracy of OCT in PID is shown. OCT is demonstrated as the substitution of biopsy in endometriosis.

Post-DeadlineChromatic dispersion compensation of an OCT system with a programmable spectral filter, Anna Yang, Frédérique Vanholsbeeck, Stephane Coen, The Univ. of Auckland (New Zealand); Jochen Schroeder,. . . . . . . . . . . . . . . [8091-76]We demonst ra te the use o f a programmable optical spectral filter to compensate all orders of chromatic dispersion in an all-fibre Fourier-domain optical coherence tomography system at 1550 nm. The point-spread-function, originally 58 micron wide, asymmetric, with strong sidelobes, is successfully made symmetric and recompressed to 38 micron, close to the theoretical limit of 36 micron.

Pathogenesis of the dry eye syndrome observed by optical coherence tomography in vitro, Oya Kray, Markus Lenz, Felix Spöler, Stefan Kray, RWTH Aachen (Germany); Norbert Schrage, Aachen Center of Technology Transfer in Ophthalmology (Germany); Heinrich Kurz, RWTH Aachen (Germany) . . . . . . . . [8091-77]Three dimensional optical coherence tomography (OCT) is introduced as a valuable tool to analyze the pathogenesis of corneal diseases. Here, OCT in combination with a novel in vitro model for the dry eye syndrome (DES) enables an improved understanding of the underlying damaging process of the ocular surface.

Quantitative measurement of cellular refractive index using spectral domain optical coherence phase microscopy, Rehman Ansari, Yoko Miura, Gereon Hüttmann, Achim Schweikard, Univ. zu Lübeck (Germany) . . . . . . . . . . . . . . . [8091-78]Quantitative measurement of cellular refractive index in live cells with high sensitivity provides a new functional contrast for Optical Coherence Phase Microscopy. Additionally, localized and transient dynamic changes in cellular refractive index allows for investigation of cellular physiology and pathogenesis.



Conference 8092Medical Laser

Applications and Laser-Tissue Interactions V

Session Chairs: Lothar D. Lilge, Ontario Cancer Institute

(Canada); Ronald Sroka, Ludwig-Maximilians-Univ. München

(Germany)

Histopathological analysis of necrosis induced in normal rat liver tissues after combined treatment between PDT and diode laser with 810nm, Raquel F. Rego, Gustavo Nicolodelli, Univ. de São Paulo (Brazil); Fernando M. Araujo-Moreira, Univ. Federal de São Carlos (Brazil); Vanderlei Salvador Bagnato, Univ. de São Paulo (Brazil) . . . . . . . . . [8092-46]Diode laser with 810nm has been used for telangiectasias treatment due the property to promote sclerosis of small blood vessels. So, in order to understand how local changes in blood flow may interfere with photodynamic response, we performed a histopathological analysis of necrosis induced in normal rats liver tissues after a combined treatment between PDT and Diode laser using three types of photosensitization (PS: topical ALA, intravenous ALA and Photogem) and different conditions of treatment (only diode laser, PS+ diode laser, diode laser + PS+ PDT, PS+ PDT+ diode laser, PS+ diode laser + PDT). Results suggest that photosensitization followed by PDT and diode laser showed a greater necrosis than other studied conditions and topical ALA presented the deeper necrosis. Therefore, the action of diode laser may reduce the photodynamic response.

Quantitative analysis of endovenous laser treatment based on human vein optical properties, Kunio Awazu, Saki Nozoe, Norihiro Honda, Katsunori Ishii, Osaka Univ. (Japan) . . . . . . . [8092-47]A simple color separation technique for solar tissue photocoagulation, Nídia P. Batista, Dawei Liang, Univ. Nova de Lisboa (Portugal) . . [8092-49]A i m i n g a t s t u d y i n g s o l a r photocoagulation in biological tissue with both the warm and the cold portions of solar spectrum, a simple color separation technique is proposed. The use of the chromatic aberration characteristic of Fresnel lens is exploited to achieve color separation by a plane mirror with a large central elliptical hole, reflecting the warm solar radiation above 600nm to one fused silica light guide, while allowing the passage of the cold radiation to another guide. ZEMAXβ ray-tracing code is used to optimize the performance of each optical component. The ex vivo measurement is performed on chicken breasts and a strong dependence of the penetration depth on wavelength has been observed.

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In vivo N-acetyl cysteine reduce hepatocyte death by induced acetaminophen, Chih-Ju Lin, Feng-Chieh Li, Sheng-Shun Wang, National Taiwan Univ. (Taiwan); Hsuan-Shu Lee, National Taiwan Univ. (Taiwan) and National Taiwan Univ. Hospital (Taiwan); Chen-Yuan Dong, National Taiwan Univ. (Taiwan) . . . . . . . . . . . . [8092-50]Acetaminophen (APAP) is the famous drug in global, and taking overdose Acetaminophen will intake hepatic cell injure. In this issue, mice are injected APAP overdose to damage hepatocyte. APAP deplete glutathione and ATP of cell, N-Acetyl Cysteine plays an important role to protect hepatocytes be injury. N-Acetyl Cysteine provides mitochondrial to produce glutathione to release drug effect hepatocyte. By 6-carboxyfluorescein diacetate (6-CFDA) metabolism in vivo, glutathione keep depleting to observe the hepatocyte morphology in time. Without NAC, cell necrosis increase to plasma membrane damage to release 6-CFDA, that’s rupture. After 6-CFDA injection, fluorescence will be retained in hepatocyte. For cell retain with NAC and without NAC are almost the same. With NAC, the number of cell rupture decreases about 75%.

Photoswitchable bactericidal effects from novel silica-coated silver nanoparticles, Gustavo Fuertes, Esteban Pedrueza, Kamal Abderrafi, Rafael Abargues, Orlando Sánchez, Juan Martínez-Pastor, Jesús Salgado, Ernesto Jimenez Villar, Univ. de València (Spain) . . . . . . . . . . . [8092-51]The antibacterial activity of novel silver nanoparticles coated with silica, Ag@silica, can be photoswitched. Ag@silica bind readily to bacterial cells without affecting their growing. However, upon simultaneous exposure to light corresponding to the SPR absorption band of Ag@silica, bacterial death is enhanced selectively on the irradiated zone.

Micromanipulation of cells and microparticles using optical fibers, Domna Kotsifaki, National Technical Univ. of Athens (Greece) . . . . [8092-52]In this work we develop a single beam optical fiber trapping system integrated with an optical fiber ablation system for micromanipulation micro objects. We verified that inserting the optical fibers at an angle played an important role for a strong three- dimensional optical trapping and ablation.

Light distribution in turbid media for photodynamic therapy: a simple approach for dosimetry studies, Lilian T. Moriyama, Clóvis Grecco, Cristina Kurachi, Vanderlei Salvador Bagnato, Univ. de São Paulo (Brazil) . . [8092-53]This study aims to develop a simple method of recovering light distribution prof i le w i th in turb id media for Photodynamic Therapy applications from measurements of light distribution in turbid optical phantoms when a narrow and collimated laser beam is used as light source.




Spectroscopic and photochemical properties of Curcumin-based photosensitizer, Francisco Rego Filho, Maria Tereza de Araujo, Univ. Federal de Alagoas (Brazil); Cristina Kurachi, Vanderlei Salvador Bagnato, Univ. de São Paulo (Brazil) . . . . . . . . . [8092-54]The aim of this study is to ful ly characterize spectroscopically and photochemically a Curcumin-based molecule to testify its possible use a photosensitizer. This is achieved by absorpt ion and f luorescence spectroscopy measurements during photobleaching, on three different concentrations whether or not on the presence of singlet oxygen quencher Sodium Azide.

FEM modeling and histological analyses on thermal damage induced in facial skin resurfacing procedure with different CO2 laser pulse duration, Francesca Rossi, Istituto di Fisica Applicata Nello Carrara (Italy); Tiziano Zingoni, Emiliano Di Cicco, Leonardo Manetti, El.En. S.p.A. (Italy); Roberto Pini, Istituto di Fisica Applicata Nello Carrara (Italy); Damiano Fortuna, El.En. S.p.A. (Italy) . . . . . . . . . [8092-55]The photothermal effects induced in the facial skin by a CO2 laser having different pulse durations were studied both theoretically and experimentally. The results were used to optimize laser settings for dermatological surgery applications, such as facial skin rejuvenation.

Synchronously pumped OPO for generation of picosecond pulses around 6.5 μm using AgGaS2 and CdSiP2, Nordine Hendaoui, Ernest Kakoudgi, Mani Aladin, Christophe Silien, André G. Peremans, Vincent Bruyninckx, Facultes Univ. Notre Dame de la Paix (Belgium); Adolfo Esteban-Martin, Majid Ebrahim-Zadeh, ICFO - Instituto de Ciencias Fotónicas (Spain); Stefan Been, Rudolf M. Verdaasdonk, Univ. Medical Ctr. Utrecht (Netherlands); Peter G. Schunemann, Kevin T. Zawilski, BAE Systems (USA); Valentin P. Petrov, Max-Born-Institut für Nichtlineare Optik und Kurzzeitspektroskopie (Germany) . . . . . . . . . . . . . . . [8092-56]We compare the performances of cadmium silicon phosphide (CSP) with with those of silver thiogallate in a singly resonant OPO, synchronously pumped by a Nd:YAG laser. We demonstrate the feasibility of a CSP-based OPO at 6.5µm for medical applications as evidenced by preliminary ablation tests on biological tissues

Evaluation of LED therapy at 945 nm on bone repair by micro x-ray fluorescence spectroscopy and scanning electron microscopy, Ana Maria M. do Espirito Santo, Univ. Federal de São Paulo (Brazil); Alexandre Greca Diamantino, Renata Amadei Nicolau, Marco Antonio de Oliveira, Univ. do Vale do Paraíba (Brazil) . . . . . . . . . . . . . . . . . . [8092-57]Phototherapy using LED at 945 nm has been employed during the regeneration process of surgically promoted femoral lesions in male rats. The characterization of the neo-formed region was performed by histomorphometric analysis, electron scanning microscopy, bone densitometry and micro x-ray fluorescence. The irradiation promoted acceleration of bone repair.

Effect of wavelength on the laser ablation threshold of hydroxylapatite irradiated by means of a pulsed nanosecond optical parametric oscillator, Vincent Bruyninckx, Laurent Lamard, Guy Terwagne, André G. Peremans, Facultes Univ. Notre Dame de la Paix (Belgium); Marc Tielemans, Daniel Heysselaer, Samir Nammour, Univ. de Liège (Belgium) . . . . [8092-58]This study shows the influence of wavelength on the laser ablation threshold of hydroxylapatite in the 2.2 - 3.0 µm range, using as tunable source a KTP optical parametric oscillator (OPO) pumped by a 10-Hz pulsed nanosecond Nd:YAG laser. OPO tunability, together with water spray on the target, allow to match wavelength to an absorption peak around 2.75 µm, thus obtaining better ablation rates than with existing fixed-wavelength Er:YAG and Er:YSGG sources at respectively 2.94 and 2.79 µm. Such an OPO-based system therefore shows promise for precise and efficient dental enamel drilling.

Viability for the conjugate use of electrosurgery and photodynamic therapy, Francisco Rego Filho, Maria Tereza de Araujo, Univ. Federal de Alagoas (Brazil); Edson Vieira, WEM Equipamentos Eletrônicos Ltda. (Brazil); Cristina Kurachi, Vanderlei Salvador Bagnato, Univ. de São Paulo (Brazil) . . . . . . . . . . . . . . . . . . [8092-59]In order to propose a new Photodynamic Therapy (PDT) protocol, a technique combining Electrosurgery and PDT was studied in terms of the optical, histopathologic and the order of conjugation of both techniques so as to improve the treated tissue volume.

Influence of the repetition rate at the morphological structure of teeth, restorative materials, and bovine femur after femtosecond laser ablation, Gustavo Nicolodelli, Rosane de Fátima Zanirato Lizarelli, Jeison T. Tribioli, Cristina Kurachi, Vanderlei Salvador Bagnato, Univ. de São Paulo (Brazil) . . . . . . . . . . . . . . . . . . [8092-60]The aim of this study was an analysis of the morphological aspects of different biological tissues irradiated by femtosecond laser pulses. A comparative study showed the ablation’s efficiency of the femtosecond lasers in processing hard tissues and the possibility of use these systems free of thermal and mechanic damage.

Protoporphyrin IX production and photobleaching during treatment of condyloma by HPV with methyl aminolevulinate, Natalia M. Inada, Mardoqueu Martins da Costa, Elizeu da Silva Ribeiro, Cristina Kurachi, Wellington Lombardi, Vanderlei Salvador Bagnato, Univ. de São Paulo (Brazil) . . . . . . . . . . . . . . . . . . [8092-61]Objective: to improve the feasibility of the Photodynamic Therapy (PDT) in the treatment of condyloma by HPV. Design: a multicentre non-randomised clinical trial. Setting: Unit of Public Health of Araraquara, Sao Paulo. Population or Sample: a total of 40 women with different grades of warts caused by HPV.

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SESSION 4

Room: B13 Tues. 17.00 to 18.00

Biospectroscopy and POC Diagnostics II

Session Chair: Bing Yu, Duke Univ. (USA)

SESSION 10


Clinical ApplicationsSession Chairs: Adam Liebert, Institute of

Biocybernetics and Biomedical Engineering (Poland); Turgut Durduran, ICFO - Instituto

de Ciencias Fotónicas (Spain)

17.00: Breast density assessment by means of time domain optical mammography at 635-1060 nm, Paola Taroni, Antonio Pifferi, Giovanna Quarto, Politecnico di Milano (Italy); Lorenzo Spinelli, Istituto di Fotonica e Nanotecnologie (Italy); Alessandro Torricelli, Rinaldo Cubeddu, Politecnico di Milano (Italy); Francesca Abbate, Anna M. Villa, Nicola Balestreri, Simona Menna, Enrico Cassano, Istituto Europeo di Oncologia (Italy) . . . . . . . . . . . . [8088-51]Breast density assessment is performed by means of time-resolved 7-wavelength (635-1060 nm) optical mammography. Good correlation was achieved between mammographic density and optical indexes in a clinical study that has involved 63 subjects up to now.

17.15: Quantitative assessment of indocyanine green concentration in healthy and cancerous breast tissue, Axel J. Hagen, Dirk Grosenick, Tim Gnoerrlich, Physikalisch-Technische Bundesanstalt (Germany); Alexander Pöllinger, Susen Burock, Peter M. Schlag, Charité Universitätsmedizin Berlin (Germany); Herbert H. Rinneberg, Rainer Macdonald, Physikalisch-Technische Bundesanstalt (Germany) . . . . . . . . . . . . . . . . . . . . . . . . . . [8088-52]Using indocyanine green (ICG) we recorded time-resolved fluorescence mammograms in order to quantitatively determine ICG enrichment in tumors and to qualitatively assess permeability of tumor microvasculature. From the analysis of the in vivo data for one case presented here, an ICG enrichment of 7.5 was found for the carcinoma.

17.30: Oxygenation of the calf muscle during an incremental, intermittent walking exercise assessed by NIRS, Sascha Härtel, Claire Kutzner, Karlsruher Institut für Technologie (Germany); Svenja Grieger, Dankiel Schneider, RheinAhrCampus Remagen (Germany); Michael Neumaier, Karlsruher Institut für Technologie (Germany); Matthias Kohl-Bareis, RheinAhrCampus Remagen (Germany) . . . . . . . . . . . . . . . . . . . . . . . . . . [8088-53]We use NIRS for the non-invasive assessment of calf oxygenation during a new walking protocol in healthy subjects of different fitness levels and discuss a measurement parameter linked to blood flow rather than oxygenation. All findings are compared with spirometry and the effect of training is discussed.

SESSION 2

Room: 5 Tues. 17.00 to 18.00

Optical Coherence MicroscopySession Chair: Theo Lasser, Ecole

Polytechnique Fédérale de Lausanne (Switzerland)

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Imaging III

8091: Room 13BOptical Coherence Tomography

and Coherence Techniques V

17.00: Combination of dark-field optical coherence microscopy with epi- fluorescence microscopy for functional cell imaging (Invited Paper), Christophe Pache, Nadia Halidi, Arno Bouwens, Martin Villiger, Jean-Jacques Meister, Theo Lasser, Ecole Polytechnique Fédérale de Lausanne (Switzerland) . . . . . . . . . . . . . [8091-04]We apply the high sensitivity and fast imaging speed of dark-field Optical Coherence Microscopy (dfOCM) to the detection of processes related to calcium ions in smooth muscle cells. To provide a better understanding of specificity of the scattering signal, a multi-modal setup combining epi-fluorescence microscopy with dfOCM was designed.

17.00: Collagen matrices as a more suitable model for in vitro study of live cells using Raman microspectroscopy (Invited Paper), Franck Bonnier, Peter Knief, Aidan D. Meade, Jennifer Dorney, Kunal Bhattacharya, Fiona M. Lyng, Hugh J. Byrne, Dublin Institute of Technology (Ireland) . . . . . . . . . . . . . . . . . . . . . . . . . . . . [8087-12]The feasibility to use 3D collagen matrices for the study of live cells using Raman microspectroscopy has been evaluated. This model presents two major improvements, first the preprocessing is simplified due to the minimised contribution of the substrate in the data recorded and secondly the collagen matrices mimic the extracellular matrix and therefore represent a more accurate model for in vitro study of living cells

17.30: Optical characterization and feasibility study of multifunctional polylactic-co-glycolic acid (PLGA) nanoparticles designed for photo-thermal optical coherence tomography, Hrebesh M. Subhash, Oregon Health & Science Univ. (USA) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . [8091-05]Nanoparticles with plasmon-resonance absorption in the near-IR (NIR) optical range are of great interest in optical coherence tomography (OCT) for contrast enhancement and diagnostic interventions in molecular imaging. In this study, we characterized the optical properties of multifunctional NIR dye-loaded PLGA nanoparticles (approved by the U.S. Food and Drug Administration) to assess the feasibility of using contrast agent for photo-thermal OCT (PT-OCT) imaging.

17.45: Contrast modification for low-coherence interference microscopy by Fourier-plane filtering, Stefan E. Schausberger, Johannes Kepler Univ. Linz (Austria) . . . . . . . . . . . . . . . . . . . [8091-06]We propose a novel full-field low-coherence interference (LCI) microscope which can provide different contrast modes using Fourier-plane filtering by means of a spatial light modulator. By altering the phase and spatial frequencies we are able to change the contrast in the depth-resolved LCI images.

17.30: Numerical simulations for quantitative spectroscopic imaging in the sidestream dark-field microscopy configuration, Annemarie Nadort, Ton G. van Leeuwen, Dirk J. Faber, Academisch Medisch Ctr. (Netherlands) . . . . . . . . . . . . .[8087-13]A novel non-invasive quantitative spectroscopy method is proposed by using spectral and structural information from sidestream dark field images. We have modeled light propagation in this geometry using numerical simulations and tissue optical phantom experiments.

17.45: Application of dynamic diffuse optical tomography in lower extremity vascular imaging, Michael A. Khalil, Hyun K. Kim, Columbia Univ. (USA); In-Kyong Kim, Rajeev Dayal, Columbia Univ. Medical Ctr. (USA); Andreas H. Hielscher, Columbia Univ. (USA) . . . . . . . . . . . . . . . . . . . . . . . . . [8088-54]Peripheral Vascular Disease (PVD) affects over 10 million Americans and is associated with significant morbidity and mortality. Diagnosis of PVD is often complicated in the diabetic and elderly population. These populations tend to have calcified arteries, which cause false negative readings. Dynamic Optical Tomography promises to overcome the limitations of current diagnostic techniques in the detection of vascular disease. We have performed initial pilot studies involving 8 PVD patients and 5 healthy volunteers. The time traces and tomographic reconstruction obtained from measurements on the feet show significant differences between healthy and affected vasculatures. Furthermore, we found that DOT is capable of identifying the disease in diabetic patients, who are misdiagnosed by traditional procedures.

Confernece End.

17.45: A biosafety study on nonlinear optical imaging, Giju Thomas, Erasmus MC (Netherlands); Oleg Nadyarnykh, Johan van Voskuilen, Hans C. Gerritsen, Utrecht Univ. (Netherlands); Henricus J. C. M. Sterenborg, Erasmus MC (Netherlands) [8087-14]Elevated peak intensity used for nonlinear imaging, generate cyclic pyrimidine dimers (CPD) that could lead to carcinogenesis. This study compares the carcinogenesis risk, by quantifying CPDs generated in CHO cells in vitro and mouse tissue in vivo, that had been exposed to femtosecond laser irradiation and radiation simulating outdoor sunlight.



SESSION 3

Room: 5 . . . . . . . Wed. 08.30 to 10.00

Polarization-Sensitive OCTSession Chair: Johannes F. de Boer, Vrije

Univ. Amsterdam (Netherlands)

SESSION 5

Room: B13 . . . . . Wed. 08.30 to 10.30

Clinical and Preclinical Tissue Characterization I

Session Chair: Franck Bonnier, Dublin Institute of Technology (Ireland)

SESSION 1

Room: 11 . . . . . . Wed. 08.30 to 10.00

Nanoparticle and NS Laser Applications

Session Chairs: Ralf Brinkmann, Univ. zu Lübeck (Germany); Martin Frenz, Univ.

Bern (Switzerland); Lothar D. Lilge, Ontario Cancer Institute (Canada)

08.30: Stabilization of the longitudinal plasmon-resonance absorption of nanorods by porous silica shell layer, Florian Rudnitzki, Gereon Hüttmann, Univ. zu Lübeck (Germany); Fulvio Ratto, Istituto di Fisica Applicata Nello Carrara (Italy) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . [8092-01]Pulsed laser irradiated gold nanoparticles can be used to modify or destroy cells and proteins. In contrast to spherical particles nanorods are not as suitable, due to transient and permanent changes of their optical properties. Here a possible optical stabilization of the nanorods by silica shells was shown and discussed.

08.45: Efficient optical trapping of gold nanoparticles, Faeghe Hajizadeh, Seyed Nader Seyed Reihani, Institute for Advanced Studies in Basic Sciences (Iran, Islamic Republic of) . [8092-02]Nowadays the applications of metallic nanoparticles are indispensable in many areas from biology to electronics. In this article, we report on stable 3-D trapping of gold nanospheres ranging from 9.5 to 254nm in diameter using a nearly-aberration-free single beam optical trap. Our experimental data, for the first time, verify the volume corrected Rayleigh model for particles smaller than 100nm in diameter. Measuring the maximum applicable force using nanoparticles, we have shown that a few tens of milli-Watts of laser power can produce pico-Newton range forces, which is ~4 times more efficient than that of the previous works.

09.00: Effect of gold nanoparticles in the local heating of skin tumors induced by phototherapy, Irene Salas-García, Félix Fanjul-Vélez, Noé Ortega-Quijano, Ángela Lavín-Castanedo, Patricia Mingo-Ortega, Univ. de Cantabria (Spain); María López-Escobar, Univ. Hospital Marques de Valdecilla (Spain); José Luis Arce-Diego, Univ. de Cantabria (Spain) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . [8092-03]This work focuses in the modeling of the thermal effects produced in Photothermal and Photodynamic therapies with gold nanoparticles to treat different skin diseases. The model permits to compare the thermal injury depending on optical power, type of pathology and the incorporation of nanoparticles in the target tissue.

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8092: Room 11Medical Laser Applications and


08.30: Speckle noise reduction by averaging in polarization sensitive spectral domain optical coherence tomography, Erich Götzinger, Michael Pircher, Bernhard Baumann, Tilman Schmoll, Harald Sattmann, Rainer Leitgeb, Christoph Hitzenberger, Medizinische Univ. Wien (Austria). . . . . . . . [8091-07]We demonstrate an ultra high speed fiber based polarization sensitive spectral domain optical coherence tomography system, using two ultra high speed CMOS line scan cameras This high speed data acquisition enables averaging of several acquired B-scans of intensity, retardation, optic axis orientation, and Stokes vectors, which strongly reduces speckle noise.08.45: Investigation of biological samples in vivo using PS-OCT system based on high-speed 1300 nm Fourier domain mode-locked laser, Marco Bonesi, Michael Pircher, Stephan Zotter, Teresa Torzicky, Erich Götzinger, Medizinische Univ. Wien (Austria); Christoph M. Eigenwillig, Benjamin R. Biedermann, Wolfgang Wieser, Robert A. Huber, Ludwig-Maximilians-Univ. München (Germany); Christoph K. Hitzenberger, Medizinische Univ. Wien (Austria) . . . . . . . . . . . . . . . . . . . . . . . . . . . . [8091-08]We present polarization-maintaining fiber-based high-resolution Fourier domain PS-OCT system based on a high-speed Fourier domain mode locked laser source at 1300 nm. Reflectivity, retardation, axis orientation and degree of polarization uniformity information are extracted from in vivo measurements of biological samples and illustrated, together with system design and performances.09.00: Retardation and depolarization imaging by polarization sensitive optical coherence tomography at 840 nm and 1030 nm, Teresa Torzicky, Erich Götzinger, Michael Pircher, Stefan Zotter, Marco Bonesi, Christoph Hitzenberger, Medizinische Univ. Wien (Austria). . . . . . . . [8091-09]Performances of polarization sensitive optical coherence tomography instruments working in the 840 nm and in the 1030 nm region were compared for imaging the ocular fundus of healthy human volunteers in vivo.

08.30: Quantitative fiberoptic spectroscopy for noninvasive, realtime medical diagnosis (Invited Paper), Henricus J. C. M. Sterenborg, Stephen C. Kanick, Erasmus MC (Netherlands); Ute Gamm, Consultant (Germany); Jan Bonne Aans, Robert L. van Veen, Dominic J. Robinson, Arjen Amelink, Erasmus MC (Netherlands) . . . . . . . . . . . . . . . . . . . . . . . . [8087-15]Optical biopsy for non-invasive diagnosis of disease is an ongoing challenge to biomedical optics. Extensive clinical studies on the measurement of optical properties of tissue using spatially resolved steady-state diffuse reflectance spectroscopy revealed that human tissues often are too inhomogeneous for diffusion theory to work accurately enough for reliable clinical diagnosis. Due to the large sampling volumes and the wavelength dependent pathlengths of the detected photons accurate measurements have appeared to be impossible in non-homogenous media. Differential Pathlength Spectroscopy, a spectroscopic measurement technique using 2 optical fibers, has been developed to overcome this problem by reducing the sampling volume and using a predetermined pathlength. Using this method the spectra obtained could be analyzed in terms of Mie and Raleigh scattering, micro vascular parameters (StO2, tHb and vessel diameter) and additional absorbers such as billirubin and beta-carotene. Recently we developed a method to estimate photon pathlengths for single fiber spectroscopy. This allows for similar spectral measurements in a much smaller diameter.

The different approaches have so far been evaluated in more than 500 patients for various clinical applications varying from surface measurements on skin and oral cavity, endoscopic measurements in bronchi, esophagus, colon and through biopsy needles in brain, breast and different types of lymph nodes.

Clinical measurements have been performed with home made scientific instruments. Recently a the University spin off company Luminostix has finished a first medical prototype that is currently in tprocess of being CE marked.

In the presentation I will give an overview of the technology involved, the challenges to get the technology into the clinic and I will discuss the latest clinical results.

09.15: Diagnostics of tumor cells by combination of Raman spectroscopy and microfluidics, Ute Neugebauer, S. Dochow, Christoph Krafft, Institut für Photonische Technologien e.V. (Germany); Thomas Bocklitz, Friedrich-Schiller-Univ. Jena (Germany); J. H. Clement, Universitätsklinikum Jena (Germany); Jürgen Popp, Institut für Photonische Technologien e.V. (Germany) and Friedrich-Schiller-Univ. Jena (Germany) . . . . . . . . . . . . . . . . . . . . . . . . . . [8087-16]

09.15: A method to calibrate phase fluctuation in polarization-sensitive swept-source optical coherence tomography, Zenghai Lu, Deepa K. Kasaragod, Stephen J. Matcher, The Univ. of Sheffield (United Kingdom) . . . . . . . . . . . . . [8091-10]We present a phase fluctuation calibration method for polarization-sensitive swept-source optical coherence tomography using a low-voltage broadband polarization modulator driven by a synchronized sinusoidal burst waveform rather than an asynchronous waveform together with the removal of the global phases of the measured Jones matrices by use of matrix normalization.

09.15: Laser treatment of tumors with nanosized thermosensitizers under the OCT control, Elena V. Zagaynova, Marina A. Sirotkina, Marina V. Shirmanova, Vadim V. Elagin, Nizhny Novgorod State Medical Academy (Russian Federation); Vladislav A. Kamensky, Institute of Applied Physics (Russian Federation); Viktor A. Nadtochenko, Nikolay N. Denisov, N.N. Semenov Institute of Chemical Physics (Russian Federation) . . . . . . . . . . . [8092-04]OCT study of accumulation of gold nanobranches into cervical carcinoma for controlled laser hyperthermia was performed. At the time of maximum accumulation hyperthermia of tumor was undertaken. Nanoparticles were shown to be effective for hyperthermia.



SESSION 3 continued09.30: A theoretical framework for the analysis of optical anisotropy in birefringent biological tissues with polarization-sensitive optical coherence tomography, Deepa K. Kasaragod, Zenghai Lu, James W. Jacobs, Stephen J. Matcher, The Univ. of Sheffield (United Kingdom) . . . . . . . . . . . . . [8091-11]Polarization-sensitive optical coherence tomography (PSOCT) has established itself as a useful and non-invasive optical imaging tool to study the birefringent biological tissues. The complex 3D structure architecture of the collagen fibers in articular cartilage is investigated using a time-domain PSOCT system and the depth-wise cumulative retardance profiles obtained is compared with a three layer cartilage model based on extended Jones matrix formulation.

SESSION 1 continued09.30: Selective excavation of decalcified dentin using a mid-infrared tunable nanosecond pulsed laser: wavelength dependency in the 6 μm wavelength range, Katsunori Ishii, Masayuki Saiki, Osaka Univ. (Japan); Kazushi Yoshikawa, Kenzo Yasuo, Kazuyo Yamamoto, Osaka Dental Univ. Hospital (Japan); Kunio Awazu, Osaka Univ. (Japan) . . . . . . . . . . . . . . . . . . . . . . . . . . . . [8092-05]Selective caries treatment is an essential application of laser dentistry. Wavelength dependency of less-invasive carious dentin excavation was investigated in 6 µm wavelength range to consider the optimal wavelength for selective excavation. Irradiation experiments indicated that the wavelength which primarily absorbed by organic matters could induce the selective excavation.

SESSION 5 continued09.30: High-resolution vital fluorescence imaging for in vivo detection of microscopic residual disease in cancer surgery, J. Quincy Brown, Jenna Mueller, Duke Univ. (USA); Jeff Mito, Duke Univ. Medical Ctr. (USA); Zachary T. Harmany, Duke Univ. (USA); David G. Kirsch, Duke Univ. Medical Ctr. (USA); Rebecca M. Willett, Duke Univ. (USA); Joseph Geradts, Duke Univ. Medical Ctr. (USA); Nimmi Ramanujam, Duke Univ. (USA) . . . . . . . . . . [8087-17]Incomplete surgical tumor resection is a significant clinical problem which leads to a reduction in survival for many cancer patients. We describe the development and validation of a methodology for high-resolution vital fluorescence imaging for detection of microscopic residual tumor in incomplete cancer resection surgeries.

09.45: Spatially modulated multispectral light to guide localized spectroscopy of lumpectomy margins, Ashley M. Laughney, Venkataramanan Krishnaswamy, Dartmouth College (USA); David J. Cuccia, Modulated Imaging Inc. (USA); Tyler B. Rice, Bruce J. Tromberg, Beckman Laser Institute and Medical Clinic (USA); Wendy A. Wells, Dartmouth Hitchcock Medical Ctr. (USA); Keith D. Paulsen, Brian W. Pogue, Dartmouth College (USA) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . [8087-18]Guided assessment of surgical margins for cancer involvement using multispectral spatially modulated structured light is validated in phantom and murine studies. Extracted optical parameter maps identify suspicious lesions in the surgical field for further characterization using a dark-field in situ scanning spectroscopy platform.

Coffee Break . . . . . . . . . . . . . . . . . . . . 10.00 to 10.30

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Clinical and Biomedical Spectroscopy and Imaging II





09.45: Novel polarization sensitive contrast agents for optical coherence tomography, Kalpesh B. Mehta, Nanguang Chen, National Univ. of Singapore (Singapore) . . . . . . . . . . . . . . [8091-12]We propose the use of double layered metallic nano-structure as polarization sensitive contrast agent for OCT. Using numerical simulation it is demonstrated that this structure has different scattering cross sections for left- and right-hand circularly polarized light. Along with the higher scattering, this polarization sensitive behavior can enhance contrast.

09.45: Mechanical response of artificial tissue models irradiated with Nd:YAG nanosecond laser pulses, Francisco G. Perez-Gutierrez, Univ. Autónoma de San Luis Potosí (Mexico); Santiago Camacho-Lopez, Ctr. de Investigación Científica y de Educación Superior de Ensenada (Mexico); Guillermo Aguilar, Univ. of California, Riverside (USA) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . [8092-06]We studied nanosecond laser induced pressure transients in tissue models. The hypothesis is that pressure transients are composed of the superposition of both: shock wave due to plasma and thermoelastic expansion due to linear absorption. We evaluate the relative contribution of each mechanism using PVDF sensors, Time-resolved shadowgraphy and interferometry.

Coffee Break . . . . . . . . . . . . . . . . . . . . 10.00 to 10.30

SESSION 4

Room: 5 . . . . . . . Wed. 10.30 to 12.30

Microcirculation ImagingSession Chair: Yoshiaki Yasuno, Univ. of

Tsukuba (Japan)

Coffee Break . . . . . . . . . . . . . . . . . . . . 10.00 to 10.30

10.30: Double-beam Doppler optical coherence tomography for visualizing the microvasculature within the human retina (Invited Paper), Stefan Zotter, Michael Pircher, Teresa Torzicky, Marco Bonesi, Erich Götzinger, Rainer A. Leitgeb, Christoph K. Hitzenberger, Medizinische Univ. Wien (Austria) . . . . . . . . . . . . . . . . . . . . . . . . . . . . [8091-13]In this work we present a double-beam phase resolved Doppler optical coherence tomography system which is capable of visualizing simultaneously the larger vessels and the microvasculature within the human retina in vivo.

10.30: Quantitative spectral imaging for intraoperative breast tumor margin assessment (Invited Paper), Bing Yu, J. Quincy Brown, Justin Y. Lo, Torre M. Bydlon, Henry L. Fu, Stephanie A. Kennedy, Marlee Junker, Duke Univ. (USA); Thomas F. Kuech, Univ. of Wisconsin-Madison (USA); Joseph Geradts, Lee Wilke, Nirmala Ramanujam, Duke Univ. (USA) . . . . . . . . . .[8087-19]This presentation provides an overview of our effort on multiple strategies in developing quantitative spectral imaging for intraoperative detection of residual cancer at the margins of excised breast tumors.


10.30: Coherent anti-Stokes Raman scattering of myelin in live mice with a micro-objective, Joël Crépeau, Erik Bélanger, Centre de recherche Université Laval Robert-Giffard (CRULRG), Université Laval (Canada) and Centre d’Optique, Photonique et Laser (COPL), Université Laval (Canada); Sophie Laffray, Centre de Recherche Université Laval Robert-Giffard (CRULRG), Université Laval (Canada); Yves De Koninck, Centre de Recherche Université Laval Robert-Giffard (CRULRG), Université Laval (Canada) and Centre d’Optique, Photonique et Laser (COPL), Université Laval (Canada); Réal Vallée, Centre de Recherche Université Laval Robert-Giffard (CRULRG), Université Laval (Canada); Daniel Côté, Centre de Recherche Université Laval Robert-Giffard (CRULRG), Université Laval (Canada) and Centre d’Optique, Photonique et Laser (COPL), Université Laval (Canada) . . . . . . . . . . [8092-62]We report the use of a commercial micro objective to obtain high quality myelin images from coherent anti-Stokes Raman scattering (CARS) lipid contrast in live mice spinal cord. The technique is sensitive to small variations of the myelin thickness, as would be observed in demyelinating pathologies.

SESSION 6

Room: B13 . . . . . Wed. 10.30 to 12.30

Clinical and Preclinical Tissue Characterization II

Session Chair: Henricus J. C. M. Sterenborg, Erasmus MC (Netherlands)

SESSION 2

Room: 11 . . . . . . Wed. 10.30 to 12.30

Post-Deadline Session and Student Awards

Session Chairs: Stefan Andersson-Engels, Lund Univ. (Sweden); Irene Georgakoudi,

Tufts Univ. (United States)


SESSION 6 continued11.00: Breast imaging using the Twente photoacoustic mammoscope: new clinical measurements, Michelle Heijblom, Univ. Twente (Netherlands) and Medisch Spectrum Twente (Netherlands); Daniele Piras, Wenfeng Xia, Johan van Hespen, Univ. Twente (Netherlands); Joost Klaase M.D., Frank van den Engh M.D., Medisch Spectrum Twente (Netherlands); Ton van Leeuwen, Univ. Twente (Netherlands) and Academisch Medisch Ctr. (Netherlands); Wiendelt Steenbergen, Srirang Manohar, Univ. Twente (Netherlands) . . . .[8087-20]Conventional breast imaging often not successfully discriminates benign from malignant tissue. To assess the feasibility of photoacoustic mammography, a new study with the Twente Photoacoustic Mammoscope has been started. Photoacoustic images of various types of breast lesions will be compared with conventional imaging and histopathology to define photoacoustic malignancy markers.

11.15: Devising an endoluminal bimodal probe which combines autofluorescence and reflectance spectroscopy with high-resolution MRI for early stage colorectal cancer diagnosis: technique, feasibility and preliminary in-vivo (rabbit) results, Anoop Ramgolam, Raphael Sablong, CREATIS-LRMN INSA (France); Hervé Saint-Jalmes, Univ. de Rennes 1 (France); Benyebka Bou-Saïd, Institut National des Sciences Appliquées de Lyon (France); Sandrine Bouvard, CERMEP (France); Olivier Beuf, CREATIS-LRMN INSA (France) . . . . . . . . . . . . . . . . . . . . . . . . . . . . [8087-21]We hereby present the conception of a second more advanced prototype of an endoluminal bimodal probe for the diagnosis of early stage colorectal cancer by combining High-Resolution MRI to autofluorescence and reflectance spectroscopy. The promising preliminary in-vivo results (rabbit) are also presented.

SESSION 4 continued11.00: High speed and high penetration Doppler optical coherence tomography (Invited Paper), Young-Joo Hong, Shuichi Makita, Univ. of Tsukuba (Japan); Myeong-Jin Ju, Byeong-Ha Lee, Gwangju Institute of Science and Technology (Korea, Republic of); Masahiro Miura, Tokyo Medical Univ. Kasumigaura Hospital (Japan); Yoshiaki Yasuno, Univ. of Tsukuba (Japan) [8091-14]High-speed and high-penetration swept-source optical coherence tomography system is developed with 1-µm short cavity laser. Doppler OCT processing is applied with custom made OCT phase stabilization algorithm which doesn’t sacrifice measurement range not as like a conventional phase stabilization mirror. The bidirectional blood flow in deep choroid was obtained.

SESSION 2 continued10.45: Increased tissue contrast by high resolution simultaneous dual-band optical coherence tomography in three dimensions, Stefan Kray, Markus Lenz, Felix Spöler, Heinrich Kurz, RWTH Aachen (Germany) . . . . . . . . . [8092-63]We demonstrate enhanced visualization of tissue contrast and morphological boundaries by analyzing three dimensional OCT volume data in two distinct wavelength regions simultaneously.

11.00: Detection of bacterial infection with a fiber optic microendoscope, Kristen C. Maitland, Nooman Mufti, Texas A&M Univ. (United States); Ying Kong, Jeffrey D. Cirillo, Texas A&M Health Science Center (United States) . . . . . . . . . [8092-64]We present the use of a fiber optic microendoscope to image and quantify bacterial infection in the skin and lungs using an animal model. Subcutaneous and intra-tracheal infections of fluorescent Mycobacterium bovis Bacillus Calmette-Guérin (BCG) bacteria were detected in situ from inocula down to 10^4 and 10^7 colony forming units, respectively.

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11.15: Optical switching and time-sequential coherent detection of markers through opposing lenses enables multicolor 3D-nanoscopy with 10-nm resolution of large intracellular volumes, Alexander Egner, Laser-Lab. Göttingen e.V. (Germany); Daniel Aquino, Andreas Schönle, Max-Planck_Institute for Biophysical Chemistry (Germany); Claudia Geisler, Laser-Lab. Göttingen e.V. (Germany); Christian A. Wurm, Stefan W. Hell, Max-Planck_Institute for Biophysical Chemistry (Germany) . . . . . . . . . . . . . . . . . . . . . . . . . . [8092-65]We demonstrate three-dimensional superresolution imaging of stochastically switched fluorophores distributed across whole cells. By evaluating the higher moments of the diffraction spot provided by a 4Pi detection scheme, single markers can be simultaneously localized with < 10 nm accuracy in 3D within a layer of 650 nm thickness at any depth within the sample.

11.30: Improving time domain fluorescence lifetime imaging with an adaptive Monte Carlo data inflation (AMDI) algorithm, Aymeric Leray, Dave Trinel, Corentin Spriet, Interdisciplinary Research Institute (France); Yves Usson, Joseph Fourier University (France); Laurent Heliot, Interdisciplinary Research Institute (France) [8092-66]We propose an original approach based on a statistical data inflation method allowing estimating accurately fluorescence lifetime with reduced acquisition time and thus minimizing cellular stress phenomena. By reducing the commonly used spatial binning factor, our approach also improves the spatial resolution of the FLIM images.

11.30: Ultrahigh-speed swept-source / Fourier domain OCT for Doppler measurements of total retinal blood flow, Bernhard Baumann, Benjamin M. Potsaid, Martin F. Kraus, Jonathan J. Liu, Massachusetts Institute of Technology (USA); David M. Huang, Oregon Health & Science Univ. (USA); Joachim Hornegger, Friedrich-Alexander-Univ. Erlangen-Nürnberg (Germany); Jay S. Duker, Tufts Univ. (USA); James G. Fujimoto, Massachusetts Institute of Technology (USA) . . . . . . . . . . . [8091-15]We present ultrahigh speed swept source / Fourier domain optical coherence tomography (OCT) operating at 200,000 axial scans per seconds for volumetric retinal Doppler flow imaging. In addition to 3D assessment of the retinal structure, a method for quantitative measurement of retinal blood flow is demonstrated.

11.45: Automated extraction of 3D Doppler OCT signatures using a support vector machine, Amardeep S. G. Singh, Tilman Schmoll, Rainer A. Leitgeb, Medizinische Univ. Wien (Austria) [8091-16]We present an automated extraction of Doppler OCT information by using a support vector machine that combines different features for classification. We employ histogram equalization that makes it possible to distinguish vessels from bulk tissue by texture analysis. This method is particularly applicable to settings with significant phase noise.

11.30: The use of infrared spectroscopy to estimate the gross biochemistry associated with different colorectal pathologies, James J. Wood, Tim Cook, Neil Shepherd, Nicholas Stone, Catherine A. Kendall, Gloucestershire Hospitals NHS Foundation Trust (United Kingdom) . . . . . . [8087-22]This study explored the use of infrared spectroscopic mapping on tissue sections of different colorectal pathology types. Dot product analysis and ordinary least squares analysis were used to quantify the distributions of component biochemicals across tissue sections through comparison with purchased biochemical spectra.

11.45: Development of optical fiber head probes for infrared endoscopic medical diagnosis, Marie-Laure Brandily, Bruno Bureau, Catherine Boussard-Pledel, Univ. de Rennes 1 (France); Olivier Loreal, l’Hôpital Pontchaillou (France); Valérie Monbet, Jacques Lucas, Univ. de Rennes 1 (France) [8087-23]Fiber Evanescent Wave Spectroscopy (FEWS) has proved to be a powerful technology for the study of biomedical samples. Recent progress in the quality and the design of chalcogenide fibers have enabled to obtain optical fiber with a miniaturized head-probe which can be used for endoscopic measurements. FEWS coupled to chemometric methods, such as PCA and PLS, allowed to differentiate between, for instance, healthy and non healthy tissue on human body biological liquid.

11.45: Single beam quantitative phase contrast 3D microscopy of cells, Vani K. Chhaniwal, University of Stuttgart (Germany); Arun Anand, Maharaja Sayajirao Univ. of Baroda (India); Ahmad Faridian, Giancarlo Pedrini, Wolfgang Osten, University of Stuttgart (Germany); Bahram Javidi, University of Connecticut (United States) . [8092-67]Phase contrast 3D microscopy of living cells provides important information on their dynamics and morphological changes. A single beam phase retrieval technique for quantitative phase contrast imaging of living cells using multiple intensity samplings of a volume speckle field is described.



SESSION 2 continued12.00: Towards a real-time microbiopsy assessment with full-field OCT, Adriano Burcheri, Ecole Supérieure de Physique et de Chimie Industrielles (France); Fabrice Harms, LLTech SAS (France); Osnath Assayag, Ecole Supérieure de Physique et de Chimie Industrielles (France); Vincent Servois, Brigitte Sigal-Zafrani, Institut Curie (France); Claude Boccara, Ecole Supérieure de Physique et de Chimie Industrielles (France) and LLTech SAS (France) . . . . . . . . . . . . . . . . . . . . . . . . . . . . [8092-68]A technique that can rapidly image a specimen without any staining could potentially provide an aid for targeting suspicious lesions. A compact full-field OCT system (1µm resolution) was tested under clinical conditions : 16 specimens have been examined (kidney, lung and breast tissues). Characteristic features of malignancy have been identified.

Student Award Presentations . . . . . 12.15 to 12.30

SESSION 6 continued12.00: Identification of dysplastic cervical cells in unstained Pap smear slides using light scattering spectroscopy, Kristin Lane, Martin Hunter, Edmund Cibas, Irene Georgakoudi, Tufts Univ. (USA) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . [8087-24]We present the use of light scattering spectroscopy as a tool to discriminate between dysplastic and normal human cervical epithelial cells smeared on a slide following traditional isolation procedures used during a Pap smear.

SESSION 4 continued12.00: Intra and inter frame differential Doppler imaging, Tilman Schmoll, Ioana R. Ivascu, Amardeep S. G. Singh, Angelika Unterhuber, Rainer A. Leitgeb, Medizinische Univ. Wien (Austria) . . . . . . . . . . . . . . . . . . . . . . . . . . . . [8091-17]We present a method to contrast the blood flow of retinal vessels from the surrounding static tissue. It is based on the extinction of the interference fringes by phase shifts of π. If moving particles of the sample introduce an additional phase shift, the signal from these particles will, in contrast to the signal from static tissue, not be attenuated. Different shifting schemes allow contrasting different flow speeds. We demonstrate its capability to contrast the different flow speeds within the human retina; from the fast flows in the optic nerve head region to the slow flows within the capillary network surrounding the fovea.

12.15: Enhanced joint spectral and time domain optical coherence tomography for quantitative flow velocity measurement, Julia Walther, Edmund Koch, Technische Univ. Dresden (Germany) . . . . . . . . . . . . . . . . . . . . . . . . . . [8091-18]In this study, an enhanced algorithm for joint spectral and time domain optical coherence tomography (STdOCT) permitting a more precise flow velocity measurement in comparison to the conventional STdOCT is presented. The comparison of the enhanced STdOCT and phase-resolved Doppler OCT shows only marginal differences in the processed velocity profiles.

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12.15: Fluorescence lifetime imaging endoscopy, Gordon T. Kennedy, Ian H. Munro, Sergio Coda, Alex J. Thompson, Imperial College London (United Kingdom); Bertrand Viellerobe, Francois Lacombe, Mauna Kea Technologies (France); Andrew Thillainayagam, Mark A. A. Neil, Gordon W. Stamp, Daniel S. Elson, Chris Dunsby, Paul M. W. French, Imperial College London (United Kingdom) [8087-25]We describe portable fluorescence lifetime imaging (FLIM) endoscopes being developed for in vivo applications. For depth-resolved imaging with subcellular resolution, we employ confocal FLIM endomicroscopy and image Förster resonance energy transfer in live cells. For tissue screening and guided biopsy applications, we have developed wide-field FLIM endoscopes for handheld operation.

Lunch/Exhibition Break . . . . . . . . . . . . 12.30 to 14.00

SESSION 7

Room: B13 . . . . . Wed. 14.00 to 16.00

Clinical and Preclinical Tissue Characterization III

Session Chair: Lise Lyngsnes Randeberg, Norwegian Univ. of Science and Technology

(Norway)

Lunch/Exhibition Break . . . . . . . . . . . . 12.30 to 14.00

SESSION 5

Room: 5 . . . . . . . Wed. 14.00 to 15.15

Advanced Data Processing and Signal Enhancement

Session Chair: Rainer A. Leitgeb, Medizinische Univ. Wien (Austria)

Lunch Break . . . . . . . . . . . . . . . . . . . . 12.30 to 14.00

SESSION 3

Room: 11 . . . . . . Wed. 14.00 to 15.30

Photodynamic InvestigationSession Chairs: Herbert Stepp, Ludwig-Maximilians-Univ. München (Germany);

Lothar D. Lilge, Ontario Cancer Institute (Canada); Stefan Andersson-Engels, Lund

Univ. (Sweden)

14.00: Near-infrared quantum dot laser induces irreversible production of reactive oxygen species in different cell types, Sergei G. Sokolovski, Svetlana A. Zolotovskaya, Neil Z. Stewart, Celine Pourreyron, Andrew P. South, Irwin McLean, Edik U. Rafailov, Univ. of Dundee (United Kingdom) . . . . . . . . . . . . . . . . . . . . . . . . . . . [8092-07]Molecular oxygen direct photoactivation by a diode laser emitting at 1260 nm is demonstrated in anoxia solutions and living cells. The laser-induced calcium-dependent oxidative stress in HeLa, HaCaT, and primary keratinocytes is observed.

14.00: Image registration based 3D-OCT motion correction and signal enhancement using orthogonal raster scans, Martin F. Kraus, Friedrich-Alexander-Univ. Erlangen-Nürnberg (Germany); Benjamin M. Potsaid, Bernhard Baumann, Jonathan Liu, Massachusetts Institute of Technology (USA); Lauren Branchini, New England Eye Ctr. (USA); Markus A. Mayer, Rüdiger Bock, Friedrich-Alexander-Univ. Erlangen-Nürnberg (Germany); Jay S. Duker, New England Eye Ctr. (USA); James G. Fujimoto, Massachusetts Institute of Technology (USA); Joachim Hornegger, Friedrich-Alexander-Univ. Erlangen-Nürnberg (Germany) . . . . [8091-19]We present a software based correction method that corrects artifacts resulting from motion during 3D-OCT imaging of the eye. It uses scans with orthogonal fast scan axis directions. After correction the volumes are merged to form a high-quality data set. Results indicate better visual quality and successful motion correction.

14.15: Mechanical compression for contrasting OCT images of biotissues, Mikhail Y. Kirillin, Pavel D. Argba, Vladislav A. Kamensky, Institute of Applied Physics (Russian Federation) . . . . . . . . . . [8091-20]Contrasting of biotissue layers in OCT images after application of mechanical compression is discussed. The study is performed by Monte Carlo simulations, for ex vivo samples, and in vivo for human volunteers.

14.00: Nano-sensitizers for multi-modality optical diagnostic imaging and therapy of cancer (Invited Paper), Malini C. Olivo, National Univ. of Ireland, Galway (Ireland) and Royal College of Surgeons Ireland (Ireland) and National Univ. of Singapore (Singapore) . . . . . . . . . . . . . . . . [8087-26]We report novel nano-sensitizers as specific oral cancer optical probes for the detection and monitoring of cancer progression in a orthotopic tumor model using advanced state-of-the-art multi-modality in-vivo optical imaging technology such as in-vivo 3D confocal fluorescence endomicroscopic imaging, optical coherence tomography (OCT) with improved optical contrast using nano-gold and Surface Enhanced Raman Scattering (SERS) based imaging and bio-sensing. These techniques can be used in tandem or independently as in-vivo optical biopsy techniques to specifically detect and monitor oral cancer cells in-vivo. These nano-sensitisers can also act as tumour specific therapeutic photosensitizers for photodynamic therapy of oral cancer coupled with additional photothermal effects rendered by plasmonic heating effects of gold nanoparticles. This nanosensitizer based optical biopsy imaging technique has the potential to provide an alternative to tissue biopsy and will enable clinicians to make real-time diagnosis, determine surgical margins during operative procedures and perform targeted treatment of oral cancer.

14.15: Photodynamic therapy prediction model using fluorescence spectroscopy and study of treatment parameters, José D. Vollet Filho, Lilian T. Moriyama, Clóvis Grecco, Univ. de São Paulo (Brazil); Juliana Ferreira, Univ. do Vale do Paraíba (Brazil); Cristina Kurachi, Vanderlei S. Bagnato, Univ. de São Paulo (Brazil) . . . . . . . . . . . . . . . . . . [8092-08]A model to predict Photodynamic Therapy outcome using photosensitizer fluorescence information, assessed through spectroscopy, was developed. Studies on treatment parameters were performed, based on the obtained model, and these results show evidence of the model validity.

Best Student Paper and Best Student Poster

Sponsored by:

Best Student Paper/PosterDeveloping Nation

Sponsored by:



SESSION 7 continued14.45: Monte Carlo simulation for cerebral structure detection by using diffuse optical imaging, Ching-Cheng Chuang, Chia-Yen Lee, Chung-Ming Chen, National Taiwan Univ. (Taiwan); Yao-Sheng Hsieh, Chun-Yang Wang, National Chiao Tung Univ. (Taiwan); Chia-Wei Sun, National Yang-Ming Univ. (Taiwan) . . . . . . . . . . . . . . . . . . . [8087-27]In this study, the 3D normal adult and AD brain were modeled from in vivo MRI image to investigate the AD-related changes in the structural properties of brain atrophy with time-resolved 3D Monte Carlo simulation. The result implies the feasibility of near-infrared imaging to diagnosis the structural characteristic of brain atrophy in AD patients.

SESSION 3 continuedSESSION 5 continued

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14.30: Speckle reduction in optical coherence tomography using elastic and viscoelastic properties of tissue, Brendan F. Kennedy, Andrea Curatolo, The Univ. of Western Australia (Australia); Timothy R. Hillman, Massachusetts Institute of Technology (USA); David D. Sampson, The Univ. of Western Australia (Australia) . . . . . . . . . . . .[8091-21]We present two techniques, strain and creep compounding, to reduce speckle in OCT images based on the elasticity and viscoelasticity of samples. We obtain a 1.52-fold reduction in contrast ratio (CR) for RTV silicone using strain compounding and a 1.65-fold reduction in CR for human lymph nodes using creep compounding.

14.45: Fourier domain optical coherence tomography axial resolution improvement with modulated deconvolution, Evgenia Bousi, Ismini Charalambous, Costas Pitris, Univ. of Cyprus (Cyprus) . . . . . . . . . . . . . . . . . . . . . . . . . . . .[8091-22]Axial resolution in Fourier Domain Optical Coherence Tomography can be improved by ~7x without the need for a broader bandwidth light source using modulated deconvolution. This method relies the beating, which appears when adding signals with slightly different frequencies, and deconvolution with the encoded source autocorrelation function.

15.00: Dispersion compensation in spectral domain optical coherence tomography in the continuum of fractional Fourier domains, Norman Lippok, Poul Nielsen, Frédérique Vanholsbeeck, The Univ. of Auckland (New Zealand) . . . . . . . .[8091-23]We present a new method of numerical dispersion compensation in spectral domain optical coherence tomography based on the fractional Fourier transform. The dispersion induced by a 26 mm length water cell was compensated for a bandwidth of 110 nm that corresponds to an axial resolution in air of 3.6 µm.

14.30: A model-based comparison of implicit and direct dosimetry for ALA-PDT of skin, Baochang Liu, McMaster Univ. (Canada); Thomas J. Farrell, Michael S. Patterson, Juravinski Cancer Ctr. (Canada) . . . . . . . . . . . . . . . . . . . . . . . . . . . [8092-09]The correlations of photosensizitizer fluorescence photobleaching and singlet oxygen luminescence (SOL) signals to the average cumulative singlet oxygen concentration in dermis are compared for different drug distributions and excitation wavelengths using a dynamic model for ALA-PDT of skin. The results show that SOL is a more robust basis for dosimetry.

14.45: PD and PDT for hepatoblastoma? Preclinical considerations, Herbert Stepp, Florian Bergmann, Ann Johansson, Michael Heide, Ludwig-Maximilians-Univ. München (Germany); Roman Metzger, Udo Rolle, Holger Till, Universitätsklinikum Leipzig (Germany) . . . . . . . . . . . . . . . . . . . . [8092-10]Intra-operative fluorescence detection or PDT of resection margins or metastatic spread of early childhood tumours might be very valuable. HuH6-tumours in rats showed selective accumulation of 5-aminolevulinic acid induced Protoporphyrin IX and good phototoxic response.

15.00: Clinical study using photodynamic action for microbial control in periodontics, Jucaira S. Giusti, Carla R. Fontana, Daniela Gonçalves, Cristina Kurachi, Vanderlei Salvador Bagnato, Univ. de São Paulo (Brazil) . . . . . . . . . . . . . . . . . . . . . . . . [8092-11]This clinical study with forty patients tested different types and concentrations of photosensitizers and LED lights on periodontal treatment using the depth probe of periodontal pocket as parameter of evaluation. Results shown that all tested groups had better results when compared with control group .

15.00: Dynamic changes in skin chromophore concentrations and skin temperatures using hyperspectral and thermal imaging techniques, Herke Jan Noordmans, John H. Klaessens, Rowland de Roode, Rudolf M. Verdaasdonk, Univ. Medical Ctr. Utrecht (Netherlands) . . . . . . . . . . . . . . [8087-28]Different hyper-spectral and thermographic techniques are assessed for medical applications. Examples are given of seeing the focus of epilepsy using hyper-spectral oxygenation imaging and assessing nerve blocks in anaesthesiology using hyper-spectral and thermographic imaging.

15.15: Intraoperative 5-ALA fluorescence detection in intramedullary tumors, Petr Zelenkov, Ivan Shevelev, Aleksandr Potapov, Denis Golbin, Nikolay Konovalov, Artyom Gousha, Anton Nazarenko, Jurii Kuszel, Daniil Rotin, N.N. Burdenko Neurosurgical Institute (Russian Federation); Sergey G. Kuzmin, State Research Ctr. NIOPIK (Russian Federation); Viktor B. Loschenov, Pavel N. Grachev, Tatjana Saveljeva, A. M. Prokhorov General Physics Institute (Russian Federation) . . . . . . . . . . . [8087-29]Little is known about utility of fluorescence guidance with 5-ALA in surgery of intramedullary tumors. In present study 15 patients were included. Bright visible fluorescence was observed in ependymomas and glioblastoma. 5-ALA-induced fluorescence guidance may be a helpful modality in surgery of intramedullary tumors.

Coffee Break . . . . . . . . . . . . . . . . . . . . 15.30 to 16.00

Coffee Break . . . . . . . . . . . . . . . . . . . .15.15 to 16.00 15.15: Construction of an array of LEDs coupled to a concentrator for phototherapy, Joana L. Almeida, Dawei Liang, Univ. Nova de Lisboa (Portugal) . . . . . . . . . . . . . . . . . . . . . . . . . . . [8092-12]To study phototherapeutic effects of red light emitted by LEDs, a simple optical concentrator capable of both efficient LED light concentration and homogenization was developed. Anti-inflammatory effects on cutaneous hyperpigmented marks were observed.

Coffee Break . . . . . . . . . . . . . . . . . . . . 15.30 to 16.00



SESSION 4

Room: 11 . . . . . . . Wed. 16.00 to 18.15

Laser-Assisted Detection Techniques

Session Chairs: Raimund Hibst, Univ. Ulm (Germany); Wolfgang Baeumler, Univ. Clinics

Regensburg (Germany)

16.00: Sentinel lymph node detection by an optical method using scattered photons (Invited Paper), Franklin Tellier, Univ. de Strasbourg (France); Herve Simon, Eurorad S.A. (France); François X. Blé, Rasata Ravelo, Renée Chabrier, Jerome Steibel, Univ. de Strasbourg (France); Jean François Rodier, Les Centres de Lutte Contre le Cancer (France); Patrick Poulet, Univ. de Strasbourg (France) . . . . . . . . . . . . . . . . . . . . . . . . . . . . .[8092-13]We present ex-vivo results obtained with near infrared optical probe for the sentinel lymph node detection, based on the recording of scattered photons at two wavelengths. A second probe with four wavelengths is developed and its preliminary results are compared to the first ones.

SESSION 8

Room: B13 . . . . . Wed. 16.00 to 18.00

Clinical and Preclinical Tissue Characterization IV

Session Chair: Malini C. Olivo, National Univ. of Ireland, Galway (Ireland)

SESSION 6

Room: 5 . . . . . . . Wed. 16.00 to 18.00

Technological AdvancesSession Chair: Robert A. Huber, Ludwig-

Maximilians-Univ. München (Germany)

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16.00: Megahertz retinal OCT: advanced data processing protocols enabled by densely sampled ultrawide-field data (Invited Paper), Thomas Klein, Wolfgang Wieser, Christoph M. Eigenwillig, Benjamin R. Biedermann, Robert A. Huber, Ludwig-Maximilians-Univ. München (Germany) . . . . . . . . . . . . . . . . . . . . . . . . . . [8091-24]We present retinal optical coherence tomography imaging at up to 1.37MHz axial scan rate with a Fourier-domain mode locked laser operating around 1050nm. The isotropically sampled data sets with ~70° ultrawide-field of view enable advanced sectioning and averaging methods.

16.00: Raman, FTIR and CARS microspectroscopy for detection of brain metastasis, Norbert Bergner, Institut für Photonische Technologien e.V. (Germany); Bernd F. M. Romeike, Universitätsklinikum Jena (Germany); Rupert Reichart, Rolf Kalff, Friedrich-Schiller-Univ. Jena (Germany); Tobias Meyer, Institut für Photonische Technologien e.V. (Germany); Benjamin Dietzek, Jürgen Popp, Institut für Photonische Technologien e.V. (Germany) and Friedrich-Schiller-Univ. Jena (Germany) . . . . [8087-30]16.15: Fluorescence wavelength-time matrix acquisition for biomedical tissue diagnostics, William R. Lloyd III, Robert H. Wilson, Leng-Chun Chen, Univ. of Michigan (USA); Gregory D. Gillispie, Fluorescence Innovations, Inc. (USA); Mary-Ann Mycek, Univ. of Michigan (USA) . . . . . . . . . . [8087-31]A specialized transient digitizer system was developed to collect fluorescence wavelength-time matrices from biological tissues. The system is compact and offers rapid collection of high signal-to-noise time- and wavelength- resolved fluorescence. Extracting wavelength-resolved fluorophore lifetimes illustrates the potential of the technology to resolve relative fluorophore contributions in a clinical setting.

16.30: Imaging of experimental rheumatoid arthritis using a new polymer-conjugated NIR-dye, Bernd Ebert, Carmen Weissbach, Thomas Dziekan, Jan Voigt, Rainer Macdonald, Physikalisch-Technische Bundesanstalt (Germany); Ines Gemeinhardt, Charité Universitätsmedizin Berlin (Germany); Kai Licha, Pia Welker, Joern Berger, Michael Schirner, mivenion GmbH (Germany) . . . . . . . . . . . . . . . . . . . . . . . . . . . [8087-32]An in-house developed device for imaging of experimental rheumatoid arthritis in the NIR spectral range following intravenous administration of a newly developed, targeted contrast agent will be presented. Temporal behavior as well as spatial distribution of fluorescence intensity are successfully utilized to differentiate healthy and inflamed ankle joints.

16.45: Dynamic multiphoton imaging of reversible and irreversible thermal changes in collagen tissues, Vladimir A. Hovhannisyan, Ping-Jung Su, Chen-Yuan Dong, National Taiwan Univ. (Taiwan) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . [8087-33]Thermal demage of collagen tissues were investigated with the use of time-lapse multiphoton microscopy and SHG photometry in the 18-90°C temperature range. Activation energy and rate constant characterized thermally-induced transitions in collagen in different tissues were estimated. Partially reversible thermal change in collagen morphology was registered using SHG imaging.

17.00: Hyperspectral video endoscope for intra-surgery tissue classification using auto-fluorescence and reflectance spectroscopy, Thomas Arnold, Martin De Biasio, Raimund Leitner, Carinthian Tech Research AG (Austria) . . . . [8087-34]This paper presents a hyper-spectral video endoscopy system which utilizes a combination of auto-fluorescence imaging and white-light reflectance spectroscopy for intra-surgery tumor classification. Results show that hyper-spectral video endoscopy exhibits a large potential to become an important imaging technology for medical imaging devices that provide additional diagnostic information about the tissue under investigation.

16.30: Spectral-domain optical coherence tomography with an arrayed waveguide grating spectrometer, Duc V. Nguyen, Academisch Medisch Ctr. (Netherlands); Imran B. Akca, Kerstin Wörhoff, René M. de Ridder, Markus Pollnau, Univ. Twente (Netherlands); Ton G. van Leeuwen, Jeroen Kalkman, Academisch Medisch Ctr. (Netherlands) . . . . . . . . . . . . . . . . . . . . . . . .[8091-25]We designed and fabricated an arrayed waveguide grating (AWG) with 2.1cmx2.6cm footprint. Using the AWG as spectrometer in a spectral-domain optical coherence tomography (OCT) set-up we demonstrate OCT imaging up to the maximum depth of 1 mm with 19 µm spatial resolution in air and in a multi-layered phantom.

16.30: Multimodal multiphoton CARS tomography, Karsten König, Hans George Breunig, JenLab GmbH (Germany); Mihaela Balu, Tatjana Krasieva, Bruce J. Tromberg, Beckman Laser Institute and Medical Clinic (USA); Wolfram Sterry, Jürgen M. Lademann, Charité Universitätsmedizin Berlin (Germany) [8092-14]Multiphoton tomography is used in hospitals and cosmetic companies to provide optical skin biopsies based on autofluorescence/SHG. CARS imaging detects non-fluorescent cosmetic agents and intratissue lipids. We report on hybrid multimodal laser imaging systems.

16.45: Low dispersion integrated Michelson interferometer on silicon on insulator for optical coherence tomography, Gunay Yurtsever, Katarzyna Komorowska, Roel Baets, Univ. Gent (Belgium) . . . . . . . . . . . . . . . . . . . . . . . . . . . [8091-26]We present an integrated sil icon Michelson interferometer for OCT fabricated with wafer scale deep UV lithography. Silicon waveguides of the interferometer are designed with GVD less than 10 ps/nm.km. The footprint of the device is 0.5 mm x 3 mm.

16.45: Skin autofluorescence photo-bleaching and photo-memory, Janis Lesins, Univ. of Latvia (Latvia) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .[8092-15]Photo-bleaching of in-vivo skin autofluorescence intensity under continuous low power laser irradiation has been studied. Fiber optic spectrometry set was used at continuous 405 nm and 532 nm laser irradiation. The fluorescence bleaching curves during skin irradiation have been obtained and analyzed. Skin photo-memory effect showing signs of low-power laser irradiation on in-vivo skin is discussed, as well.

17.00: Mesoscopic 3D optical phantom technology and its application in optical coherence tomography, Andrea Curatolo, Brendan F. Kennedy, David D. Sampson, The Univ. of Western Australia (Australia) . . . . . . . . . . . . [8091-27]We present novel mesoscopic 3D structured phantoms for use in OCT experiments. The fabrication process involves a master by photolithography, bearing the imprint of the desired structure and a two-stage PDMS silicone casting process. Optical scattering contrast is introduced using different concentrations of TiO2 particles in the two castings. In this paper, the utility of the phantoms is validated in the analysis of speckle reduction.

17.00: Resolution limits between two embedded objects using an optical frequency-domain method: a numerical approach, Vianney M. Piron, Jean-Pierre L’Huillier, Ecole Nationale Supérieure d’Arts et Métiers (France) . . . . . . . . . . . . . . . [8092-16]We have used a FEM to model the light propagation in biological tissues (slab). FFT of the time-dependent impulse transmitted responses were computed. The phase delay was analyzed versus the distance between two objects simulating tumors, whereas the resolution limits were estimated considering different working modulation frequencies (100MHz to 1GHz) and tacking into account the noise level.



SESSION 4 continued17.15: Efficiency of an accelerated hybrid method for steady-state spatially resolved reflectance simulations in multilayered tissue-like media, Chemseddine Mansouri, Ernest Galbrun, Walter C. P. M. Blondel, Ecole Nationale Supérieure d’Electricité et de Mécanique - Nancy (France) . . . . . . . . . . [8092-17]In this work, the Monte Carlo calculations MC had been speeded up by using efficient computational hybrid technique computing on graphics processing units (GPU). This method effectively reduces the simulation time by a factor of 8 compared to GPU-based MC code.

SESSION 8 continued17.15: Morphological and molecular analysis of the collagen fibers in inflammatory process, Luis Felipe C. Carvalho, Univ. Federal do ABC (Brazil); Mônica Alves, São José dos Campos Dental School (Brazil); Carlos A. Soares, Univ. Federal do ABC (Brazil); Janete D. Almeida, São José dos Campos Dental School (Brazil); Herculano da Silva Martinho, Univ. Federal do ABC (Brazil) . . . . . . . . . . . . [8087-35]The aimed is study the structural changes of collagen from inflammatory processes analyzing the histopathology and Raman spectra. Molecular analysis of samples by Raman spectroscopy showed significant differences related mainly to the vibrational modes o proline, hydroxyproline and CH3/CH2, the level of information provided by morphological analysis is limited.

17.30: Optical spectroscopy for quantitative sensing in human pancreatic tissues, Robert H. Wilson, Malavika Chandra, William R. Lloyd III, Leng-Chun Chen, Univ. of Michigan (USA); James Scheiman, Diane Simeone, Barbara McKenna, Univ. of Michigan Health System (USA); Mary-Ann Mycek, Univ. of Michigan (USA) . . . . . . . . . . . . . . . . [8087-36]The first collection of wavelength-resolved reflectance and fluorescence spectra and time-resolved fluorescence decay curves from human pancreatic tissues was acquired with clinically-compatible instrumentation. Mathematical models of reflectance and fluorescence extracted parameters related to tissue morphology and biochemistry that were statistically significant for distinguishing between pancreatic tissue types.

17.45: Near-infrared Raman spectroscopy for detection of gastric cancer peritoneal dissemination in vivo, Jun Ma, Ocean Univ. of China (China); Weizheng Mao, Qingdao Univ. (China); Ming Xu, Longjing Gong, Yuan Gao, Ronger Zheng, Ocean Univ. of China (China) . . . . . . . . . . . . . . . . . . [8087-37]The gastric cancer peritoneal dissemination nude mice model was set up in our research. The spectra of different tissues and classifications of cancer were detected by near-infrared Raman spectroscopy system in imitating laparotomy. The data were classified by SVM algorithm to diagnose cancer foci furthermore.

SESSION 6 continued17.15: Real-time massively parallel processing of spectral optical coherence tomography data on graphics processing units, Marcin Sylwestrzak, Maciej Szkulmowski, Daniel Szlag, Piotr Targowski, Nicolaus Copernicus Univ. (Poland) . . . . . . [8091-28]In this contribution we present Spectral Optical Coherence Tomography (SOCT) biomedical imaging using massively parallel data processing on GPU. We describe the software taking into consideration data flow, parallel processing and organization of threads. For illustration we show examples of high resolution, real time imaging of human tissue.

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17.30: Programmable picosecond laser for swept-source OCT, Guy Lamouche, Sébastien Vergnole, National Research Council Canada (Canada); Youngjae Kim, Bryan Burgoyne, Alain Villeneuve, Genia Photonics Inc. (Canada) . [8091-29]A unique and novel, fully programmable picosecond laser source that offers complete tailoring of the wavelength sweep that benefits SS-OCT applications is presented. Special features like linear sweep in wavenumber and variable sweep rates are experimentally demonstrated with a prototype centered around 1.5 micrometers.

17.30: Determination of the optical properties of porcine dura-mater tissue considering the anisotropy factor, Marie-Theres Heine, René Michels, Alwin Kienle, Univ. Ulm (Germany) [8092-18]The anisotropy factor of porcine dura mater is determined by goniometric measurements. This result is used to determine the absorption and reduced scattering coefficients of the tissue using an integrating sphere measurement and Monte Carlo simulations.

17.45: Dual excitation waveform Fabry-Pérot tunable filters used in swept sources, Irina Trifanov, Multiwave Photonics (Portugal); Adrian Bradu, Liviu Neagu, Univ. of Kent (United Kingdom); Antonio B. Lobo Ribeiro, Univ. Fernando Pessoa (Portugal); Adrian G. Podoleanu, Univ. of Kent (United Kingdom) . . . . . . . . . . . . . . . . . . . . [8091-30]We report experimental evidence of quenching the resonances of Fabry-Perot tunable filter used as a wavelength selective element in swept sources OCT systems. The method is based on applying a non-sinusoidal, synthesized waveform to the tunable filter, waveform that can be found experimentally. A significant improvement in the OCT image quality has been obtained without any software recalibration method.

17.45: Portable semiconductor disk laser for in vivo tissue monitoring: a platform for the development of clinical applications, Rodrigo A. Aviles-Espinosa, ICFO - Instituto de Ciencias Fotónicas (Spain); George Filippidis, Foundation for Research and Technology-Hellas (Greece); Craig Hamilton, M Squared Lasers Ltd. (United Kingdom) and Solus Technologies Ltd. (United Kingdom); Graeme Malcolm, M Squared Lasers Ltd. (United Kingdom); Kurt J. Weingarten, Time-Bandwidth Products AG (Switzerland); Thomas Südmeyer, Yohan Barbarin, Ursula Keller, ETH Zurich (Switzerland); David Artigas-García, ICFO - Instituto de Ciencias Fotónicas (Spain) and Univ. Politècnica de Catalunya (Spain); Pablo Loza-Alvarez, ICFO - Instituto de Ciencias Fotónicas (Spain) . . . . . . . . . . . . . . [8092-19]The potential use of a portable (140x240x70 mm) picosecond semiconductor disk laser (modelocked by a quantum-dot semiconductor saturable-absorber mirror) for nonlinear imaging applications is demonstrated. Optimized laser-tissue interaction in GFP labeled neurons is achieved using low peak powers and its center wavelength (965 nm) virtually matching GFP action cross section.

18.00: Plasmonic manipulations of fluorescence proteins using single femtosecond pulses, Gili Bisker, Limor Minai, Dvir Yelin, Technion-Israel Institute of Technology (Israel) . . . . . . . . . . . [8092-20]Gold nanospheres irradiated with intense femtosecond pulses are demonstrated useful for altering the functionality and the structure of green fluorescent protein. We experimentally show that a single pulse illuminating a mixture of the protein with 20 nm diameter particles induced a significant fluorescence reduction due to massive protein aggregation.



SESSION 9

Room: B13 . . . . Thurs. 08.30 to 10.30

Skin Diagnostics and Therapy ISession Chair: Martin C. Fischer, Duke Univ.

(USA)

SESSION 5

Room: 11 . . . . . Thurs. 08.30 to 10.00

Biomodulation and BloodSession Chairs: Ronald Sroka, Ludwig-Maximilians-Univ. München (Germany);

Lothar D. Lilge, Ontario Cancer Institute (Canada)

08.30: Red blood cell micromanipulation with elliptical laser beam profile optical tweezers in different osmolarity conditions, Mersini I. Makropoulou-Loukogiannaki, Ellas Spyratou, Alexandros A. Serafetinides, National Technical Univ. of Athens (Greece) . . . . . . . . . . . . . . . . . . . [8092-21]In this work, we study the behaviour of normal RBC under optical tweezers with elliptical laser beam in different osmolarity conditions. We examined the folding or rotation, induced to RBC by light angular momentum.

08.45: In vitro analysis of low-level laser irradiation on human osteoblast-like cells proliferation, Nora Bloise, Enrico Saino, Francesca Bragheri, Paolo Minzioni, Ilaria Cristiani, Univ. degli Studi di Pavia (Italy); Marcello Imbriani, Salvatore Maugeri Foundation (Italy) and Univ. degli Studi di Pavia (Italy); Livia Visai, Univ. degli Studi di Pavia (Italy) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . [8092-22]We present the results of a study aiming to examine the in vitro effect of a single or a multiple doses of low-level laser irradiation on proliferation of the human osteosarcoma cell line, SAOS-2. The results show that by repeated irradiations it was possible to observe a marked enhancement of SAOS-2 growth.

09.00: Biochemical and topological analysis of bovine sperm cells induced by low-power laser irradiation, Thiago R. Dreyer, Univ. Federal do ABC (Brazil); Adriano Siqueira, Univ. de São Paulo (Brazil); Taciana D. Magrini, Pablo A. Fiorito, Univ. Federal do ABC (Brazil); Mayra E. Ortiz d’Avila Assumpcao, Marcilio Nichi, Univ. de São Paulo (Brazil); Herculano Martinho, Marcella P. Milazzotto, Univ. Federal do ABC (Brazil) . . . . . . . . . . . . . . . . . . . . . . . . . [8092-23]Biochemical and topological effects of 633nm low level laser were assessed by FTIR, flow cytometry and AFM. Changes on plasma, acrossomal membrane, morphological status and mitochondrial metabolism are affected by the irradiation dose. We conclude that low-level laser irradiation is an effective method to induced changes on sperm cell metabolism.

SESSION 7

Room: 5 . . . . . . Thurs. 08.30 to 10.00

Intravascular and Endoscopic OCT

Session Chair: Brett E. Bouma, Wellman Ctr. for Photomedicine (USA)T

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08.30: Endoscopic optical coherence tomography for imaging the tympanic membrane, Anke Burkhardt, Julia Walther, Peter Cimalla, Matthias Bornitz, Edmund Koch, Universitätsklinikum Carl Gustav Carus Dresden (Germany) . . . . . . . [8091-31]The intention of this research is imaging the tympanic membrane and fluids behind it for the visualization of otitis media using optical coherence tomography (OCT). The OCT scanner head was further extended by an endoscopic setup to allow access inside cavities.

08.45: Clinical evaluation of intracoronary OCT tissue characterization, Gijs van Soest, Evelyn Regar, Erasmus MC (Netherlands); Brett E. Bouma, Gary Tearney, Massachusetts General Hospital (USA); Antonius F. W. van der Steen, Erasmus MC (Netherlands) . . . . . . . . . . . . . . . . . . . . . . . . [8091-32]We present the results of a clinical evaluation of OCT tissue characterization by imaging of the optical attenuation coefficient. We demonstrate the feasibility of in vivo tissue characterization based on a clinical pilot study. A new speckle reduction technique will be discussed to enable the processing of full pullbacks.

08.30: Diagnostics and treatment of tumours using laser techniques (Invited Paper), Katarina Svanberg M.D., Lund Univ. Hospital (Sweden) . . . . . . . . . . . . . . . . . . . . . . . . . . [8087-38]Applications of optical and laser spectroscopy to the medical field, including photodynamic therapy (PDT) and laser-induced fluorescence diagnostics (LIF) for cancer treatment and diagnostics, respectively, will be presented. Photodynamic therapy in conjunction with LIF for demarcation of the treatment target area will be discussed. To overcome the limited light penetration in superficial illumination interstitial delivery (IPDT) with the light transmitted to the tumour via optical fibres has been developed. Interactive feed-back dosimetry is of importance for optimising this modality and such a concept has been developed and will be presented. Special emphasis will be on prostate cancer therapy with interstitial PDT.

The most important prognostic factor for cancer patients is early tumour discovery. If malignant tumours are detected during the non-invasive stage, most tumours show a high cure rate of more than 90 %. There is a variety of conventional diagnostic procedures, such as x

X-ray imaging. More advanced results are given in computerised investigations, such as CT-, MRI- or PET-scanning. Laser-induced fluorescence (LIF) for tissue characterisation is a technique that can be used for monitoring the biomolecular changes in tissue under transformation from normal to dysplastic and cancer tissue before structural tissue changes are seen at a later stage. The technique is based on UV or near-UV illumination for fluorescence excitation. The fluorescence from endogenous chromophores in the tissue alone, or enhanced by exogenously administered tumour seeking substances can be utilised. The technique is non-invasive and gives the results in real-time. LIF can be applied for point monitoring or in an imaging mode for larger areas, such as the vocal cords or the portio of the cervical area. The possibility to combine LIF and PDT will be discussed and illustrated with clinical examples from many specialities, such as dermatology, gynaecology and laryngology.

A new method where free gas (oxygen or water vapour) in the human sinus cavities is detected will be described. The technique is based on gas absorption spectroscopy in scattering media. The method can also be used to study the gas exchange in between the nasal cavity and the different sinuses in the facial region.

09.15: Determination of chromophore concentrations from spatially resolved skin measurements, Stefan Andree, Ingo Gersonde, Jürgen Helfmann, Laser- und Medizin-Technologie GmbH, Berlin (Germany) . . . . . . . . . . . . . . . [8087-39]Spectrally and spatially resolved skin measurements are presented. A Monte Carlo-based lookup table is used to infer optical parameters. Tissue layer structure is accounted for by using appropriate distance ranges to determine optical parameters. The effect of chemical environment on beta-carotene extinction is investigated

09.00: Automated volumetric stent analysis of in-vivo intracoronary optical coherence tomography three-dimensional datasets, Giovanni J. Ughi, Tom Adriaenssens, Kevin Onsea, Christophe Dubois, Mark Coosemans, Peter Sinnaeve, Walter Desmet, Jan D’hooge, Katholieke Univ. Leuven (Belgium) . . . . . . . . . . . . . . . . . . . . . . . . . . [8091-33]An algorithm for automated lumen and stent analysis of three-dimensional intra-coronary OCT datasets is presented. Vessel lumen and stent-struts are segmented through analysis of A-scan lines. Segmentation allows for automatic quantification of lumen area and stent apposition/coverage. The algorithm has been validated against manual assessment by two independent experts.

09.15: Quantification of macrophages in ApoE deficient mouse aorta by optical coherence tomography, Zhao Wang, Case Western Reserve Univ. (USA); Satoko Tahara, Toshifumi Morooka, Hiram G. Bezerra, Marco A. Costa, Univ. Hospitals of Cleveland (USA); Andrew M. Rollins, Case Western Reserve Univ. (USA) . . . . . . . . . . . . . . . . . . [8091-34]We evaluated the capability of OCT to detect macrophages in ApoE-/- mouse aorta and developed a fully automated method to quantify the macrophage area. Quantification by the dedicated algorithm showed a good correlation with histology. The method has the potential to be a substitute for histology for quantifying macrophages.

09.15: Pulsed-laser stimulation of the inner ear: a wavelength study, Michael Schultz, Laser Zentrum Hannover e.V. (Germany); Ingo U. Teudt, Gentiana I. Wenzel, Medizinsche Hochschule Hannover (Germany); Wolfgang A. Ertmer, Leibniz Univ. Hannover (Germany); Thomas Lenarz, Andrej Kral, Medizinsche Hochschule Hannover (Germany); Alexander Heisterkamp, Laser Zentrum Hannover e.V. (Germany). . . . . . . . . . . . . . . . . . . . . . . [8092-24]We present our results of an in-vivo wavelength study on laser stimulation with nanosecond pulses of the inner ear (cochlea). For the first time the wavelength was varied from ultraviolet to near-infrared to investigate the stimulation process in the cochlea and to relate cochlea activation to hemoglobin and water absorption.



SESSION 5 continued09.30: Monte Carlo simulation of photon way in clinical laser therapy, Iulian G. Ionita, Gabriel Voitcu, Univ. of Bucharest (Romania) . . . . . . . . . . . [8092-25]The multiple scattering of light can increase efficiency of laser treatment of inflammatory diseases by enlarging the treated area. The Monte Carlo simulation was used to fit the radial dependence of diffuse reflectance on a live phantom tissue. The effective cross section of cell was determined from absorption coefficient.

SESSION 9 continued09.30: Hyperspectral characterization of bioploymer diffusion in human skin, Julio E. Hernandez-Palacios, Univ. of Oslo (Norway); Ingvild J. Haug, Norwegian Univ. of Science and Technology (Norway); Øystein Grimstad, Univ. Hospital of Trondheim (Norway); Lise L. Randeberg, Norwegian Univ. of Science and Technology (Norway) . . . . . . . . . . . . . . . . . . . . . . . . . . .[8087-40]In this study diffusion of fluorescence tagged biopolymers in ex vivo human skin were measured using hyperspectral fluorescence imaging. Preliminary results indicate that hyperspectral imaging is well suited for such measurements in larger skin samples.

SESSION 7 continued09.30: Full-field optical coherence tomography imaging system with endoscopic probes, Anne Latrive, A. Claude Boccara, Ecole Supérieure de Physique et de Chimie Industrielles (France) . . . . . . . . . . . . . . . . . . . . . . . . . . . . [8091-35]Detecting pathologies such as cancer at an early stage requires an in-situ cellular diagnosis, which could be provided by minimally invasive imaging techniques such as Optical Coherence Tomography. We have developed an endoscopic cellular imaging system adapted from Full-Field OCT, with an entirely passive probe. The system achieves a resolution of a few microns in three dimensions, enough to distinguish cellular structures. We present images from ex vivo biological samples of cancerous and healthy human tissues.

09.45: Phantoms for intravascular or endoscopic optical coherence tomography, Charles-Etienne Bisaillon, Gordon Campbell, Marc L. Dufour, Guy Lamouche, National Research Council Canada (Canada) . . . . . . . . . . . . . . . . . . . . . . . . . . . [8091-36]We present two methods for the fabrication of multilayer tubular phantoms for optical coherence tomography. The first method uses silicone and additives that give highly durable phantoms. This method is used to produce phantoms of diseased coronary arteries. The second method uses poly(vinyl alcohol) cryogels to obtain improved mechanical properties.

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09.45: Newly developed photon-cell interactive Monte Carlo (pciMC) simulation for non-invasive and continuous diagnosis of blood during extracorporeal circulation support, Daisuke Sakota, Setsuo Takatani, Tokyo Medical and Dental Univ. (Japan) . . . . . . . . . . . . . . . . . . . . . . . . [8092-26]We developed a photon-cell interactive Monte Carlo “pciMC” model based on the geometric optics theory for photon migration in blood. The “pciMC” revealed that the forward scattering characteristics vary depending on the RBCs’ orientation and yielded accurate quantifications of hematocirt and hemoglobin of flowing blood during extracorporeal circulation.

09.45: Non-invasive imaging of skin cancer using two photon microscopy with fluorescence lifetime imaging, Rakesh Patalay, Clifford B. Talbot, Ian H. Munro, Imperial College London (United Kingdom); Hans Georg Breunig, Karsten Koenig, JenLab GmbH (Germany); Yuriy Alexandrov, Sean Warren, Mark A. Neil, Paul M. W. French, Anthony Chu, Imperial College London (United Kingdom); Gordon W. Stamp, The Royal Marsden NHS Foundation Trust (United Kingdom); Christopher Dunsby, Imperial College London (United Kingdom) . . . . . . . . . . . . . . . . . . . . . . . . . . .[8087-41]We present the clinical application of multispectral fluorescence lifetime imaging with two photon excitation for the assessment of skin cancer. Four spectral channels are used to collect fluorescence lifetimes using time-correlated single photon counting from lesions in vivo and ex vivo.

Coffee Break . . . . . . . . . . . . . . . . . . . .10.00 to 10.30 Coffee Break . . . . . . . . . . . . . . . . . . . . 10.00 to 10.30

SESSION 8

Room: 5 . . . . . . Thurs . 10 .30 to 12 .30

Biomedical Applications of OCTSession Chair: Adrian Gh . Podoleanu, Univ.

of Kent (United Kingdom)

Coffee Break . . . . . . . . . . . . . . . . . . . . 10.00 to 10.30

SESSION 6

Room: 11 . . . . . Thurs . 10 .30 to 12 .30

Clinical Laser ApplicationsSession Chairs: Carsten M . Philipp,

Elisabeth Klinik (Germany); Ronald Sroka, Ludwig-Maximilians-Univ. München

(Germany)

10.30: Photoacoustic blood vessel detection during surgical laser interventions, Jens Horstmann, Udo J. Birk, Alexander Baade, Ralf Brinkmann, Univ. zu Lübeck (Germany) . . . [8092-27]Photoacoustics is used for real-time blood vessel detection during minimally invasive, laser assisted liver interventions. In this work, experimental investigations concerning the maximum vessel detection depth are carried out and compared to numerical simulations.

SESSION 10

Room: B13 . . . . Thurs . 10 .30 to 11 .30

Skin Diagnostics and Therapy IISession Chair: Martin Fischer, Duke Univ.

(USA)

10.30: Optical biopsy on breast tissue using light-CT technology: a pilot study, Fabrice Harms, LLTECH SAS (France); Osnath Assayag, Adriano Burcheri, Ecole Supérieure de Physique et de Chimie Industrielles (France); Martine Antoine, Hôpital de Paris (France); Brigitte Sigal, Institut Curie (France); Bertrand De Poly, LLTECH SAS (France); Claude Boccara, Ecole Supérieure de Physique et de Chimie Industrielles (France) . . . . . . . . . . . . . . . . . . [8091-37]This study aims to evaluate Light-CT as a safe and efficient tool for optical biopsy on breast tissue in per operatory conditions by comparison with histological sections. Good structural correspondence is observed for most breast tissue structures.

10.45: xf-OCM for the assessment of type I diabetes, Corinne Berclaz, Joan Goulley, Christophe Pache, Arno Bouwens, Erica Martin-Williams, Martin L. Villiger, Anne Grapin-Botton, Theo Lasser, Ecole Polytechnique Fédérale de Lausanne (Switzerland) . . . . . . . . . . . . . . . . . . . . . . . . [8091-38]xf-OCM coupled with an advanced segmentation algorithm allows high resolution 3-dimensional quantification and size distribution measurement of murine islets of Langerhans for better understanding of early diabetes onset.

10.30: Differentiation of eumelanin and pheomelanin in skin lesions using transient absorption microscopy (Invited Paper), Martin C. Fischer, Thomas Matthews, Mary Jane Simpson, Ivan Piletic, Maria Selim, Warren Warren, Duke Univ. (USA) . . . . . . . . . . . . . . . . . . . . [8087-42]We can differentiate different types of melanins in human skin lesions based on differences in transient optical absorption dynamics. We report on progress towards developing a diagnostic imaging modality for melanoma.

10.45: 1318nm-diode laser-supported partial nephrectomy in laparoscopic and open surgery: preliminary results of a prospective feasibility study, Ronald Sroka, Ludwig-Maximilians-Univ. München (Germany) . . . . . . . . . . . . . . . . . . [8092-28]The aim of this study was to develop a safe and effective laser assisted partial nephrectomy technique without need for ischemia. By means of a diode laser emitting at 1318nm 13 patients suffering from kidney tumours were treated either by open surgery (n=5) or by laparoscopic approach (n=8). Laser power was set between 55 to 80W, thus kidney surgery could be performed using bare fibre technique. Coagualtion and sealing of the remaining tissue was performed in non-contact mode. While surgery and post operative healing was fine the devices for fibre guidance and for rinsing the tissue site needs to be improved.



SESSION 10 continued11.00: In vivo morphologic and spectroscopic investigation of psoriasis, Dimitrios Kapsokalyvas, Riccardo Cicchi, Nicola Bruscino, Univ. degli Studi di Firenze (Italy); Domenico Alfieri, Light4tech Firenze S.r.l. (Italy); Daniela Massi, Torello Lotti, Francesco S. Pavone, Univ. degli Studi di Firenze (Italy) . [8087-43]Psoriasis is an autoimmune disease of the skin. In vivo investigation with optical means was performed. A novel Dermoscopic method revealed the macroscopic characteristics. High resolution images with a Multiphoton Microscope revealed characteristic morphologic features in the subcellular level.

SESSION 6 continued11.00: In vivo study of partial liver resection on pigs using a 1.9 μm thulium fiber laser, Dirk Theisen-Kunde, Univ. zu Lübeck (Germany); Heike Wolken, Univ. Schleswig-Holstein (Germany); Veit Danicke, Ralf Brinkmann, Univ. zu Lübeck (Germany); Hans-Peter Bruch, Markus Kleemann, Univ. Schleswig-Holstein (Germany) . . . . . [8092-29]Partial resection of the liver was performed in 3 pigs with a 1.9 µm Thulium fiber laser system. Hemostasis was highly sufficient so blood lost and bile leakage was negligible. During 3 weeks survival period no complications occurred. Histology showed ongoing scar formation after 3 weeks.

11.15: In vivo laser assisted end-to-end anastomosis with ICG-loaded chitosan patches, Francesca Rossi, Paolo Matteini, Istituto di Fisica Applicata Nello Carrara (Italy); Giuseppe Esposito, Alba Scerrati, Alessio Albanese, Alfredo Puca, Giulio Maira, Univ. Cattolica del Sacro Cuore (Italy); Giacomo Rossi, Univ. degli Studi di Camerino (Italy); Roberto Pini, Istituto di Fisica Applicata Nello Carrara (Italy) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . [8092-30]We present an experimental study on an innovative end-to-end laser assisted anastomosis performed with the application of ICG-loaded chitosan patches. All the anastomoses resulted patent and no perivascular haemorrhage was detected. Histological analyses documented an optimal vessels wall regeneration.

SESSION 8 continued11.00: Improved OCT imaging of lung tissue using a prototype for total liquid ventilation, Christian Schnabel, Sven Meissner, Edmund Koch, Technische Univ. Dresden (Germany) . . . [8091-39]Optical coherence tomography (OCT) imaging of lung tissue can be improved by liquid-filling. For in vivo imaging a total liquid ventilator was developed using perfluorodecalin as breathing fluid. The ventilator setup is described and OCT and intravital microscopy images of liquid-filled lung tissue compared to air-filled ones are presented.

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11.15: In vivo TPEF-SHG microscopy for detecting collagen remodeling after laser micro-ablative fractional resurfacing treatment, Riccardo Cicchi, Dimitrios Kapsokalyvas, Michela Troiano, Piero Campolmi, Cristiano Morini, Torello Lotti, Francesco Pavone, Univ. degli Studi di Firenze (Italy) . .[8087-44]In-vivo combined TPEF-SHG microscopy were used to detect collagen remodeling and reorganization in living subjects following laser micro-ablative fractional rejuvenation treatment. A strong age-dependence of the treatment effectiveness was found, demonstrating the capability of non-linear imaging in providing a non-invasive follow-up of a laser-based treatment.

SESSION 11

Room: B13 . . . . Thurs. 11.30 to 12.30

Clinical and Preclinical Diagnostics I

Session Chair: J. Quincy Brown, Duke Univ. (USA)

11.30: Toward clinically relevant speeds and depth in 3D microcirculation imaging (Invited Paper), Martin J. Leahy, Univ. of Limerick (Ireland) . . . . . . . . . . . . . . . . . . . . . . . . . . . . [8087-45]In recent years the ability to capture exquisite high resolution images of the microcirculation has become available in research labs [1]. Clinically deployment depends on their ability to address an unmet clinical need and clinically important events often happen over periods of seconds; heart beat, breathing, endothelial dependent and other forms of vasomotion. However, these instruments generally take several hours to render 3D images of the microcirculation and often the images require significant ‘touch-up’. The microvascular bed is a living and dynamic system where flow can change 100-fold and vessel diameter can change enormously in response to metabolic and other requirements. Indeed with the resolution now possible, one can see vessels appear and disappear (collapse), since many smaller vessels especially in the skin are normally closed. Recently, we have developed an number of techniques aimed at imaging the microcirculation in two (TiVi) [2] and three dimensions (cross-correlation OCT and an absorption-based tomographic system) [3,4] which we hope will provide imaging at clinically useful speeds and depths. This paper will introduce these techniques and highlight the challenges which currently prevent 3D microcirculation at sub-second speeds.1. Microcirculation Imaging. (Wiley-VCH), Leahy, M.J. editor, 2011.2. O’Doherty, J., McNamara, P.M., Fitzgerald, B.W. and Leahy, M. J., Dynamic microvascular responses with a high speed TiVi imaging system J. Biophotonics 1-5 (2010)3. Jonathan, E. Enfield, J., and Leahy, M.J. 2010. Correlation mapping method for generating microcirculation morphology from optical coherence tomography (OCT) intensity images. J. Biophotonics (published online 17 December 2010). 4. McNamara, P.M., Jonathan, E., O’Connell, M. and Leahy, M. J. Development of an absorption-based tomographic system for mapping the human microvasculature, SPIE BiOS Photonics West 2011 Oral, Proceedings of SPIE [7898 49].The authors would like to acknowledge the support of the IRCSET (Irish Research Council for Science, Engineering & Technology) for funding this project. This research was also supported by the National Biophotonics Imaging Platform (NBIP) Ireland funded under the Higher Education Authority PRTLI Cycle 4, co-funded by the Irish Government and the European Union - Investing in your future.

11.15: Functional optical coherence tomography in urology, Dirk J. Faber, Daniel M. de Bruin, Evelyne C. Cauberg, Kurdo Barwari, Jean J. de la Rosette, M. Laguna, Theo de Rijke, Ton G. van Leeuwen, Univ. van Amsterdam (Netherlands) . . . . . . . . . . . . . . . . . . . . . . . [8091-40]we present two ex vivo studies to evaluate functional OCT to discriminate between different classifications of normal/benign/cancerous tissue of bladder and kidney. The outcome of both studies requires additional in vivo studies, probe development for routine application of OCT in the clinic, and better understanding of the origin of the OCT signal.

11.30: Optical coherence tomography for imaging of subpleural alveolar structure using a Fourier domain mode locked laser, Lars Kirsten, Julia Walther, Peter Cimalla, Maria Gaertner, Sven Meissner, Edmund Koch, Technische Univ. Dresden (Germany) . . . . . . . . . . . . . . . . . . . . . . . . . [8091-41]The investigation of lung dynamics is a further biomedical application requiring high-speed OCT imaging techniques. Visualization of subpleural alveolar structures of the rabbit lung is presented using a Fourier domain mode locked laser based OCT system, enabling repetition rates of 49.5 kHz and 123 kHz, respectively.

11.30: Excimer laser debridement of necrotic erosions of skin without collateral damage, James J. Wynne, IBM Thomas J. Watson Research Ctr. (USA); Jerome M. Felsenstein, Dermatologist (USA); Robert Trzcinski, Donna Zupanski-Nielsen, Daniel P. Connors, IBM Thomas J. Watson Research Ctr. (USA) . . . . . . . . . . . . . . . . . . . . . . . . . . [8092-31]ArF excimer laser radiation will debride burn eschar and other dry necrotic erosions of the skin. Debridement will cease when sufficiently moist viable tissue is exposed, due to absorption by aqueous chloride ions through the non-thermal process of electron photodetachment, thereby avoiding collateral damage to the viable tissue.

11.45: Optodynamic monitoring of laser tattoo removal, Matija Jezersek, Univ. of Ljubljana (Slovenia); Ladislav Grad, Fotona d.d. (Slovenia); Toma? Po?ar, Univ. of Ljubljana (Slovenia); Boris Cencic, Irena Bacak, Fotona d.d. (Slovenia); Janez Mozina, Univ. of Ljubljana (Slovenia) . . . . . [8092-32]The goal of this research is to use the information contained in the mechanisms occurring during laser tattoo removal process. For this reason we employed a fast laser beam deflection probe (BDP) which measures the cracking sound that originates from the dye explosions in the process know as selective photothermolysis. The experiments were performed in vitro (skin phantoms), ex vivo (marking tattoos in pigs ears) and in vivo (professional and amateur decorative tattoos in several patients). The signal includes the information about the energy released during the interaction, specific for different skin and tattoo conditions.

11.45: Vulvar intraepithelial neoplasia: detection with optical coherence tomography, Ronni Wessels, Antoni van Leeuwenhoek Ziekenhuis (Netherlands); Martijn D. de Bruin, Academisch Medisch Ctr. (Netherlands); Marc van Beurden, Antoni van Leeuwenhoek Ziekenhuis (Netherlands); Dirk J. Faber, Academisch Medisch Ctr. (Netherlands); Hester van Boven, Harm van Tinteren, Antoni van Leeuwenhoek Ziekenhuis (Netherlands); Ton G. van Leeuwen, Academisch Medisch Ctr. (Netherlands); Theo J. M. Ruers, Antoni van Leeuwenhoek Ziekenhuis (Netherlands). . [8091-42]We hereby present our preliminary results of optical coherence tomography of vulvar intraepithelial neoplasia (VIN). We used the attenuation coefficient to distinguish between suspected VIN lesions and healthy tissue.



SESSION 8 continued12.00: Dental calculus image based on optical coherence tomography, Yao-Sheng Hsieh, National Chiao Tung Univ. (Taiwan); Yi-Ching Ho, Shyh-Yuan Lee, National Yang-Ming Univ. (Taiwan); Ching-Cheng Chuang, National Taiwan Univ. (Taiwan); Chun-Yang Wang, National Chiao Tung Univ. (Taiwan); Chia-Wei Sun, National Yang-Ming Univ. (Taiwan) . . . . . . . . . . . . . . . . . . . . . . [8091-43]The dental calculus was characterized and imaged by means of swept-source optical coherence tomography. The refractive indices of enamel, dentin, cementum and calculus were measured as 1.625±0.024, 1.534±0.029, 1.570±0.021 and 1.896±0.085. The dental calculus lead strong scattering property and thus the region can be identified under enamel with SSOCT imaging.

SESSION 6 continued12.00: Fat tissue histological study at NIR laser treatment of the human skin in vitro, Irina Y. Yanina, N.G. Chernyshevsky Saratov State Univ. (Russian Federation) . . . . . . . . . . . . . . . . . . [8092-33]The goal of this work is to analyze histological slices of skin samples with the subcutaneous adipose tissue after laser irradiation at different doses.

The tissue samples were irradiated by laser. After fixing the slices was made 5-7 microns were analyzed.

In preparations the signs of skin damage are expressed to a much lesser extent when regimes 1.5 W, 1.2 min or less. Major changes are localized in the dermis and presented by swelling of connective tissue fibers without their destruction.

The smallest changes were observed with minimal impact (1 W, 0.5 min).

Changes of the subcutaneous adipose tissue were not observed.

The data obtained are important for safe layer-by-layer dosimetry of laser irradiation used in the treatment of obesity and cellulite.

12.15: Dependence of ablation depth on incident angle for bony tissue ablation using pulsed CO2 laser, Yaokun Zhang, Karlsruher Institut für Technologie (Germany); Jessica Burgner, Vanderbilt Univ. (USA); Jörg Raczkowsky, Heinz Wörn, Karlsruher Institut für Technologie (Germany) . . . . . . . . . . . . . . . . . . . . . . . . . . [8092-34]An Addition Model is developed to describe the shape of the craters ablated by single pulses with non-zero incident angle. A strict definition of ablation depth is given. In the context of it, experiments are conducted and show that the ablation depth is independent of incident angle for the angles less than 30°.

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12.15: Intravitreal endoscopic OCT for retinal surgery, Grigory V. Gelikonov, Valentin V. Gelikonov, Sergey Ksenofontov, Dmitry A. Terpelov, Institute of Applied Physics (Russian Federation); Georgy Stolyarenko, Dgaser Doroshenko, Posterior Eye Section Diagnostics and Surgery Ctr. (Russian Federation) . . . . . . . . . . . . . . . . . . . . . . . . . [8091-44]We present the first clinical use endoscopic OCT device equipped with tiny 0.6 mm (23GA) probe for intravitreal real time retina in-depth imaging. GRIN optics based forward looking probe has been developed. Scanning rate of 16 fps of 1.5x1.5 sector shape image has been reached First in-vivo clinical experiments have been conducted. Results are presented.

Lunch Break . . . . . . . . . . . . . . . . . . . . 12.30 to 14.00

SESSION 9

Room: 5 . . . . . . Thurs. 14.00 to 15.30

Ophthalmic OCT TechniquesSession Chair: Wolfgang Drexler, Medizinische Univ. Wien (Austria)

12.15: Characterization of atherosclerotic plaque-depositions by infrared, Raman, and CARS microscopy, Christian Matthäus, Gero Bergner, Christoph Krafft, Institut für Photonische Technologien e.V. (Germany); Benjamin Dietzek, Friedrich-Schiller-Univ. Jena (Germany); Bernhard Brehm, Bernd F. M. Romeike, Universitätsklinikum Jena (Germany); Jürgen Popp, Institut für Photonische Technologien e.V. (Germany) and Friedrich-Schiller-Univ. Jena (Germany) . . . . . . . . . . . . . . . . . . . . . . . . . . [8087-46]

Lunch Break . . . . . . . . . . . . . . . . . . . . 12.30 to 14.00

SESSION 7

Room: 11 . . . . . Thurs. 14.00 to 15.15

Lasers in OphthalmologySession Chair: Ralf Brinkmann, Univ. zu

Lübeck (Germany)

Lunch Break . . . . . . . . . . . . . . . . . . . . 12.30 to 14.00

SESSION 12

Room: B13 . . . . Thurs. 14.00 to 15.30

Clinical and Preclinical Diagnostics II

Session Chair: Martin J. Leahy, Univ. of Limerick (Ireland)

14.00: Novel fiber tip sensor for pH measurement in gastroesophageal apparatus (Invited Paper), Francesco Baldini, Giacomo Ghini, Ambra Giannetti, Folco Senesi, Cosimo Trono, Istituto di Fisica Applicata Nello Carrara (Italy) . . . . . . . . . . . . . . . . . . . . . . . . [8087-47]A novel optical fiber sensor was designed and characterized for pH detection in the stomach, using controlled pore glasses with the pH indicator covalently bound on them immobilized at the end of the optical fibers.

14.00: Simultaneous, tracked multi-wavelength optical coherence tomography for clinical applications (Invited Paper), Boris Pova?ay, Medizinische Univ. Wien (Austria); Ingrid Böttcher, Heidelberg Engineering GmbH (Germany); Bernd Hofer, Marieh Esmaeelpour, Susanne Binder, Medizinische Univ. Wien (Austria); Ralph Engelhardt, Heidelberg Engineering GmbH (Germany); Wolfgang Drexler, Medizinische Univ. Wien (Austria) . [8091-45]Motion-tracked optical coherence tomography at two wavelength bands is applied within clinical studies of diseases affecting the retina and choroid. Different to the commonly used wavelength range of 800 nm 1060 nm radiation allows to image significant details normally hidden even in enhanced 800nm images.

14.00: Study of light scattering and transparency in human edematous corneas and application to corneal grafts, Tal Marciano, Donald Peyrot, Fatima Alahyane, Caroline Crotti, Laura Kowalczuk, Karsten Plamann, Ecole Nationale Supérieure de Techniques Avancées (France). . . . . . . . . . . . . . . . . . . . [8092-35]The outcome of ultrashort pulse laser surgery of the cornea is strongly influenced bu the light scattering properties of the tissue for which litte data is available. This study provides quantitative value of the light scattering and its relation to the degree of the edema.

14.15: Comparative studies of UV laser ablation effects on intraocular lenses, Ellas Spyratou, Constantine Bacharis, George Zoulinakis, National Technical Univ. of Athens (Greece); Eleni Tsaousi, Univ. of Ioannina (Greece); Theodoros Stroumpinis, National Technical Univ. of Athens (Greece); Ioannis Asproudis, Univ. Hospital of Ioannina (Greece); Mersini I. Makropoulou-Loukogiannaki, Alexander A. Serafetinides, National Technical Univ. of Athens (Greece) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . [8092-36]The aim of this study is the qualitative investigation of the effect of UV laser on several IOL’s polymers. Ablation experiments were conducted on IOLs with UV excimer laser radiation (λ=193 nm) and UV Nd:YAG solid state laser radiation, (λ=266 nm and λ=213 nm respectively). The morphology was examined with SEM and AFM. The photochemical, photomechanical and/or photothermal mechanism of laser ablation was argued. The interaction of the 213-nm Nd:YAG laser radiation with the polymeric material of IOL’s is under investigation.



SESSION 12 continued14.30: Transmural differences in myocardial oxygen extraction estimated by quantitative diffuse reflectance spectroscopy during coronary bypass surgery, Tobias Lindbergh, Linköping Univ. (Sweden) and Linköping Univ. Hospital (Sweden); Henrik Ahn, Zoltán Szabó, Linköping Univ. Hospital (Sweden) and Linköping Univ. (Sweden); Marcus Larsson, Tomas Strömberg, Linköping Univ. (Sweden) . . . . . . . . . . . . . . . . . . . . . . . . . . . [8087-48]Myocardial oxygenation was assessed using calibrated diffuse reflectance spectroscopy at the epicardial (surface) and subendocardial layer (intramuscular) during open-chest surgery in humans. Intramuscular oxygenation was systematically lower indicating higher oxygen extraction, demonstrating the potential of the technique within clinical and research cardiac surgery.

14.45: Characterization of cytochrome c as marker for retinal cell degeneration by uv/vis spectroscopic imaging, Julia Hollmach, Julia Schweizer, Gerald Steiner, Lilla Knels, Richard H. W. Funk, Edmund Koch, Technische Univ. Dresden (Germany) . . . . . . . . . . . . . . . . . . . . . . . . . . [8087-49]This study demonstrates the ability of characterizing the local distribution and oxidative state of cytochrome c in retinal ganglion cells under in situ conditions by uv/vis spectroscopic imaging. The results show that spectroscopic imaging in conjunction with multivariate analysis methods is a new approach for the detection of cell degeneration.

SESSION 7 continued14.30: Automatic optoacoustic dosimetry control for retinal photocoagulation, Kerstin Schlott, Medizinisches Laserzentrum Lübeck GmbH (Germany); Stefan Koinzer, Univ. Schleswig-Holstein (Germany); Lars Ptaszynski, Alex Baade, Marco Bever, Medizinisches Laserzentrum Lübeck GmbH (Germany); Johann Roider, Univ. Schleswig-Holstein (Germany); Reginald Birngruber, Medizinisches Laserzentrum Lübeck GmbH (Germany); Ralf Brinkmann, Univ. zu Lübeck (Germany) . . . [8092-37]Laser photocoagulation is a treatment for many retinal diseases. Due to pigmentation lesions are often irregular. Optoacoustics allow real time determination of temperature. The treatment laser was automatically shut off after the desired temperatures were reached in rabbit eyes in vivo. First temperature measurements on patients were performed.

14.45: Investigations of laser-induced micro bubble cluster dynamics in RPE/retinal phantoms, Andreas Fritz, Andrea Zegelin, Lars Ptaszynski, Reginald Birngruber, Ralf Brinkmann, Medizinisches Laserzentrum Lübeck GmbH (Germany) . . . . . . . . . . . . . . . . . . . . . . . . . . [8092-38]Selective retina treatment (SRT) targets RPE-related diseases by laser induced transient microbubble clusters. To investigate the damage range of these clusters, Agarose gel as a retinal phantom was used to cover RPE explants. Bubble dynamics were studied interferometrically and photographically irradiating with 3ns and 1.7µs pulses to optimize SRT parameters.

15.00: Accuracy of real-time optoacoustic temperature determination during retinal photocoagulation, Alex Baade, Kerstin Schlott, Susanne Luft, Lars Ptaszynski, Marco Bever, Reginald Birngruber, Ralf Brinkmann, Medizinisches Laserzentrum Lübeck GmbH (Germany) . . [8092-39]Optoacoustics can be used to monitor retinal temperatures during photocoagulation in vivo and in real time. Presented data shows that the error of temperature determination is less than 3°C. Preliminary results show agreement between calculated and experimental results for the conversion of the optoacoustically aquired mean to central peak temperature.

SESSION 9 continued14.30: Adaptive optics multimodal cellular resolution imaging system with retinal motion correction, Robert J. Zawadzki, UC Davis Medical Ctr. (USA); Steven M. Jones, Lawrence Livermore National Lab. (USA); Arlie G. Capps, UC Davis Medical Ctr. (USA); Brian J. Bauman, Scot S. Olivier, Lawrence Livermore National Lab. (USA); John S. Werner, UC Davis Medical Ctr. (USA) . . . . [8091-46]We summarize progress on development of the next generation of an adaptive optics multimodal retinal imaging instrument that implements aberration-free optical design, simultaneous acquisition of OCT and SLO signals and retinal motion correction. The feasibility of new instrumentation for improved visualization of microscopic retinal structures will be discussed.

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14.45: One micron high-sensitive double beam Doppler optical coherence angiography with variable velocity range, Franck Jaillon, Shuichi Makita, Yoshiaki Yasuno, Univ. of Tsukuba (Japan) . . . . . . . . . . . . . . . . . . . . . . . . . . . .[8091-47]To increase the visibility of choroidal vasculature, we here propose to modify the double beam optical coherence angiography technique. This modification consists in producing a variable beam separation. This allows to access to different velocity ranges of blood vessels without sacrificing the scanning rate.

15.00: Measuring anterior segment biomechanical properties and intraocular pressure with swept source optical coherence tomography, David Alonso-Caneiro, Karol M. Karnowski, Bartlomiej J. Kaluzny, Maciej Szkulmowski, Andrzej A. Kowalczyk, Maciej Wojtkowski, Nicolaus Copernicus Univ. (Poland) . . . . . . . . . . . . . . . . . . . . . . . . . . . .[8091-48]In this contribution the SSOCT combined with air-puff system for intraocular pressure measurements is described. Preliminary results are presented. The data analysis provides additional information on biomechanical properties of the anterior segment during non-contact tonometry measurements.

15.15: Scattering properties and transparency characterization of human corneal grafts, Olivier Casadessus, Laure Siozade-Lamoine, Gaëlle Georges, Carole Deumié-Raviol, Institut Fresnel (France); Louis Hoffart, John Conrath, Hôpital La Timone (France) . . . . . . . . . . . . . . . . . . . . .[8091-49]To improve the efficiency of corneal graft sorting for transplantation procedure purpose, we propose the consideration of new tools in order to obtain a quantitative characterization of the transparency of the cornea. Its scattering properties are studied experimentally and compared with theoretical calculations.

15.00: Detection of early metabolic alterations in the ocular fundus of diabetic patients by time-resolved autofluorescence of endogenous fluorophores, Dietrich Schweitzer, Lydia Deutsch, Friedrich-Schiller-Univ. Jena (Germany); Matthias Klemm, Technical Univ. Ilmenau (Germany); Susanne Jentsch, Martin Hammer, Jens Dawczynski, Ulrich Alfons Müller, Friedrich-Schiller-Univ. Jena (Germany) . . . . . . . . . . . . . . . . . . . . . . . . . . [8087-50]A home built laser scanner ophthalmoscope was applied for detection of metabolic alteration at the eye ground before signs of diabetic retinopathy are visible or detectable by commercial ophthalmologic devices.

15.15: Using 2D correlation and multivariate analysis combined with plasmonic effects to expand the use of Raman microspectroscopy in biomedical applications, Mónica Marro Sánchez, Saurabh Raj, Satish Rao, ICFO - Instituto de Ciencias Fotónicas (Spain); Alice Taubes, Pablo Villoslada, Institut d’Investigacions Biomèdiques Agustí Pi Sunyer (Spain); Dmitri Petrov, ICFO - Instituto de Ciencias Fotónicas (Spain) . . . [8087-51]We show that Surface Enhanced Raman spectroscopy (SERS) combined with 2D correlation and multivariate analysis provides considerable progress in using Raman microspectroscopy for cutting edge biomedical research applications such as treatment delivering in cancer living cells, the diagnosis of retina neuroinflamed tissue and the study of elastic properties of single DNA molecules.

Coffee Break . . . . . . . . . . . . . . . . . . . . 15.30 to 16.00 Coffee Break . . . . . . . . . . . . . . . . . . . .15.30 to 16.00 Coffee Break . . . . . . . . . . . . . . . . . . . . 15.30 to 16.00



SESSION 8

Room: 11 . . . . . Thurs. 16.00 to 17.45

FS Laser ApplicationsSession Chairs: Alfred Vogel, Univ. zu Lübeck (Germany); Christoph Haisch, Technische Univ. München (Germany)

16.00: High-power, diode-pumped Er:YAG lasers for soft and hard tissue applications (Invited Paper), Arne Heinrich, Pantec Biosolutions AG (Liechtenstein); Clemens Hagen, Pantec Engineering AG (Liechtenstein); Attila Vizhanyo, Peter Krammer, Stefan Summer, Simon Gross, Christof Böhler, Thomas Bragagna, Pantec Biosolutions AG (Liechtenstein) . . . . . . . . . . . . . . . . . . . . . . . [8092-40]A high power, diode-pumped Er:YAG laser platform is presented, the side-pumping by qcw laser diodes allows for easy power scalability shown by one laser system with 2 W for soft tissue applications and another with 15 W for hard tissue applications.

A portable fractional ablative laser system based on a miniaturized diode-pumped Er:YAG laser is presented. The device can operate at repetition rates up to 500 Hz and has an incorporated beam deflection unit. It is smaller, lighter and cost efficient compared to systems based on lamp-pumped lasers. Furthermore the new pulse parameters enable a variety of applications in dermatology and in general medicine.

A diode-pumped Er:YAG laser for hard tissue applications is presented. The high repetition rate with up to 15 W average output power, allows treatments otherwise not feasible with low repetition rate, lamp-pumped lasers. The variable pulse duration of 10 to 200 µs combined with the good beam quality ensures precise and fast treatment.

16.30: Femtosecond laser: the finest tool for hard tissue ablation, Iulian G. Ionita, Univ. of Bucharest (Romania); Marian Zamfirescu, National Institute for Lasers, Plasma and Radiation Physics (Romania) . . . . . . . . . . . . . . . . . . . . . . . . . . [8092-41]We report dental hard tissue ablation experiments at different fluence values using femtosecond laser. The set-up was composed by a high energy femtolaser, optical and mechanical equipment for focusing and displacement of the beam on the sample surface. Using a lens to focus the beam we have obtained fluence range between 64 J/cm2 and 0.21 J/cm2. Samples were human extracted teeth and mandible bone. Created structures were rows. Characterization of ablated structures was made by scanning electron microscopy. Ablation areas images show crystalline and regular structures. There are not evidences of material burning under 64 J/cm2. Generated structures are reproducible, dependent on tissue quality. Dimensions of structures are of tens microns, dependent on beam fluence and material hardness. We appreciate the potential of the method to about 1 micron precision. The results are positive considering the advantages of the method: ablation precision and no collateral damage.

16.45: Focal spot shaping for femtosecond laser pulse photodisruption through turbid media, Anja Hansen, Tammo Ripken, Alexander Heisterkamp, Laser Zentrum Hannover e.V. (Germany) . . [8092-42]For an optical breakdown in the posterior eye segment the aberrations of the anterior eye elements cause a distortion of the wavefront and therefore an increased threshold energy. The influence of adaptive optics on lowering the threshold energy by refining a distorted focus was investigated using time-resolved photography.

SESSION 13

Room: B13 . . . . Thurs. 16.00 to 18.00

Clinical and Preclinical Diagnostics III

Session Chair: Francesco Baldini, Istituto di Fisica Applicata Nello Carrara (Italy)

16.00: Image-based fluorescence recovery after photobleaching (FRAP) to dissect vancomycin diffusion-reaction processes in Staphylococcus aureus biofilms, Samia Daddi Qubekka, Univ. Paris-Sud 11 (France); Romain Briandet, INRA-AgroParisTech (France); François Waharte, Institut Curie (France); Marie-Pierre Fontaine-Aupart, Karine Steenkeste, Univ. Paris-Sud 11 (France) . . [8087-52]An original protocol of FRAP imaging developed by our group was applied to dissect vancomycin diffusion-reaction processes in Staphylococcus aureus biofilms. The results have enabled an advance towards the understanding of antibiotic resistance.

16.15: Time-resolved fluorescence spectroscopic investigation of cationic polymer/DNA complex formation, Cosimo D’Andrea, Andrea Bassi, Paola Taroni, Daniele Pezzoli, Alessandro Volonterio, Gabriele Candiani, Politecnico di Milano (Italy) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . [8087-53]Time-resolved fluorescence spectroscopy of the DNA dye SYBR Green I was carried out to characterize cationic polymer/DNA complex (polyplex) formation in water. Both fluorescence amplitude and lifetime proved to be very sensitive to the polymer/DNA ratio (Charge Ratio, +/-)

SESSION 10

Room: 5 . . . . . . Thurs. 16.00 to 17.15

Advanced Coherent Sensing and Imaging Concepts II

Session Chair: Maciej Wojtkowski, Nicolaus Copernicus Univ. (Poland)

16.00: Holographic optical coherence tomography, Dierck Hillmann, Christian Lührs, Thorlabs GmbH (Germany); Tim Bonin, Univ. zu Lübeck (Germany); Peter Koch, Thorlabs GmbH (Germany); Gereon Hüttmann, Univ. zu Lübeck (Germany) . . . . . . . . . . . . . . . . . . . . . . . . . . [8091-50]We demonstrate holographic full-field swept-source optical coherence tomography. By using a simple Michelson interferometer setup, a rapidly tunable laser and combining scalar diffraction theory with standard OCT signal processing we obtain depth invariant imaging quality.

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16.15: Localized measurements of quantitative, wavelength dependent optical properties by low-coherence spectroscopy, Nienke Bosschaart, Dirk J. Faber, Ton G. van Leeuwen, Maurice C. Aalders, Univ. van Amsterdam (Netherlands) . . . . . . [8091-51]Low-coherence spectroscopy (LCS) is a method for measuring spectral absorption, scattering and backscattering coefficients over a large wavelength range (480-700 nm). In this study, we demonstrate the controllability (size and location) of the probed volume in the medium by LCS, enabling highly localized optical property measurements.

16.30: Measurement of the scattering anisotropy in optical coherence tomography, Vitali M. Kodach, Dirk J. Faber, Jan van Marle, Ton G. van Leeuwen, Jeroen Kalkman, Academisch Medisch Ctr. (Netherlands) . . . . . . . . . . . . . . . . . . . . [8091-52]We demonstrate that the phase function in the backward direction can be measured in OCT measurement of scattering media. When the shape of the phase function is known, the scattering anisotropy parameter g can be determined. Our method is used to determine the g-factor of Intralipid: g=0.34±0.01.

16.30: A point-of-care system for biopsy diagnosis for differentiation of benign and malignant prostate tissue, Carola E. Gerich, Jörg L. Opitz, Susan Derenko, Thomas Härtling, Fraunhofer-Institut für Zerstörungsfreie Prüfverfahren (Germany); Marieta Toma, Mildred Sergon, Susanne Füssel, Universitätsklinikum Carl Gustav Carus Dresden (Germany); Ralf Nanke, Jens Fehre, Siemens AG (Germany); Manfred Wirth, Gustavo Baretton, Universitätsklinikum Carl Gustav Carus Dresden (Germany); Jürgen Schreiber, Tae-Young Han, Fraunhofer-Institut für Zerstörungsfreie Prüfverfahren (Germany); Sangdae Lee, IM Healthcare (Korea, Republic of) . . . . . . . . . . . . . . . . . . . . . . . . . [8087-54]The continuation of the research on differentiation of benign and malignant tissue from prostates is presented. The time dependent fluorescence measurements were improved by the TCSPC-method. The algorithms were developed concern-ing the velocity, sensitivity and automatisation of the decision procedure. The presented clinical study confirms the chosen evaluation method of the feasibility study based on non-exponential time decay. Similar good results were obtained.

16.45: Comparison of ultra-fast swept source full-field OCT and conventional scanning OCT with varying NA, Tim Bonin, Univ. zu Lübeck (Germany); Peter Koch, Thorlabs GmbH (Germany); Gereon Hüttmann, Univ. zu Lübeck (Germany) . . . [8091-53]In this paper, the image quality of high-speed swept source full-field OCT images will be compared to conventional scanning OCT systems. The effect of the absence of a confocal aperture leading to crosstalk between adjacent image points will be discussed in detail for different numerical apertures (Nas) as well as the systems lateral and axial point spread functions (PSFs).

16.45: Exhaled nitric oxide measurements, comparison between different sensors: impacts of technical factors, Julien Mandon, Radboud Univ. Nijmegen (Netherlands); Peter J. F. M. Merkus, Radboud Univ. Nijmegen Medical Ctr. (Netherlands); Simona M. Cristescu, Frans J. M. Harren, Radboud Univ. Nijmegen (Netherlands) . . . . . . . . . . . [8087-55]Several technologies for exhaled nitric oxide (NO) detection have been reported. We present an optical laser-based sensor for sub-ppbv detection level and propose an overview of the technical factors influencing exhaled NO values, including: the device used for measuring, the method of collection and the expiratory flow rate.



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SESSION 13 continued17.00: Accuracy of vessel diameter estimated from a vessel packaging compensation in diffuse reflectance spectroscopy, Ingemar Fredriksson, Marcus Larsson, Tomas Strömberg, Linköping Univ. (Sweden) . . . . . . . . . . . . . . . . . . . . . . . . . . . [8087-56]An effect known as vessel packaging influences diffuse reflectance spectroscopy recordings of tissue containing blood vessels. To compensate for this the vessel diameter is estimated when fitting measured spectra. The accuracy of this estimation for a potential use as a clinical parameter is evaluated in this paper.

17.15: DCT-SVM-based multi-classification of mouse skin precancerous stages from autofluorescence and diffuse reflectance spectra, Faiza Abdat, Ctr. de Recherche en Automatique de Nancy (France) . . . . . . . . . [8087-57]This paper deals with multiclassification of skin precancerous stages based on bimodal AutoFluorescence spectroscopy and Diffuse Reflectance spectroscopy. The proposed data processing method is based on Discrete Cosine Transform and on Support Vector Machine

SESSION 10 continued17.00: Time-domain coherence-gated Shack-Hartmann wavefront sensor, Jingyu Wang, Adrian Podoleanu, Univ. of Kent (United Kingdom) [8091-54]We investigate the possibility of narrowing the depth range of a physical Shack - Hartmann wavefront sensor by using coherence gating. Accuracy of wavefront measurements using a coherence-gated (CG)/SH-WFS was evaluated. This novel technique has the potential of providing depth resolved aberration information, which can guide better correction in adaptive optics assisted OCT and confocal imaging instruments.

Conference End

SESSION 8 continued17.00: Plasmonic targeting of cancer cells using single femtosecond pulses, Limor Minai, Lior Golan, Gili Bisker, Dvir Yelin, Technion-Israel Institute of Technology (Israel) . . . . . . . . . . . . . . . . . [8092-43]By illuminating cancer cells conjugated to gold nanospheres with femtosecond pulses tuned to the particles’ plasmonic resonance, we observed significant cell necrosis and the formation of multi-nuclei cells. Cell damage was observed even after a single pulse. The technique could be useful for effective targeting of easily accessible cancer types.

17.15: New compact femtosecond laser source for penetrating keratoplasty at 1.65 μm, Caroline Crotti, Florent Deloison, Donald A. Peyrot, Ecole Nationale Supérieure de Techniques Avancées (France); Florent Aptel, Hopital Edouard Herriot (France); Fatima Alahyane, Laura Kowalczuk, Tal Marciano, Karsten Plamann, Ecole Nationale Supérieure de Techniques Avancées (France); Michèle Savoldelli, Jean-Marc Legeais, Hôpital Hôtel Dieu (France) . . . . . . . . . . . . . . . . . . . . . . . . [8092-44]Femtosecond laser surgery is difficult in the case of pathological corneas and we suggest a better suited laser wavelength. We have developed a new simple and compact laser. The use of 1650 nm wavelength significantly improves the quality and the penetration depth of incisions, without inducing any side effets.

17.30: Interaction dynamics of FS-laser-induced cavitation bubbles and its impact on the laser/tissue interaction of modern ophthalmic laser systems, Nadine Tinne, Laser Zentrum Hannover e.V. (Germany); Holger Lubatschowski, Rowiak GmbH (Germany); Alexander Heisterkamp, Tammo Ripken, Laser Zentrum Hannover e.V. (Germany) . . . . . . . . . . . . . . . . . . . . . . . . . . [8092-45]For the first time we present a time-resolved photographic analysis of the interaction of temporally separated cavitation bubbles generated by femtosecond LIOB in water. Results are discussed concerning the cutting effect of modern femtosecond lasers.

Conference End

17.30: Visualizing gold nanorods and their fluorescence emission in breast cancer cells, Liesbeth Hartsuiker, Peter van Es, Wilma Petersen, Rajagopal Rayavarapu, Aufried Lenferink, André A. Poot, Leon W. M. M. Terstappen, Ton G. van Leeuwen, Srirang Manohar, Cees Otto, Univ. Twente (Netherlands) . . . . . . . . . . . . . . . . . . . . . . . . [8087-58]Human breast cancer cells incubated with gold nanorods (GNR) were subjected to Scanning Electron Microscopy (SEM) and Raman imaging. Raman imaging revealed both fluorescence and Raman features, spatially related to the GNR distribution. Both SEM and Raman data show that uptake of GNR results in accumulation in intracellular vacuoles.

17.45: Fluorescence spectroscopy for the detection of oral cancer: clinical study of 150 subjects, Ana L. Francisco, Univ. Estadual de Campinas (Brazil); Luciane H. Azevedo, Univ. de São Paulo (Brazil); Clovis A. Lopes Pinto, Luiz P. Kowalski, Fundação Antônio Prudente (Brazil); Cristina Kurachi, Univ. de São Paulo (Brazil) [8087-59]Fluorescence spectroscopy using 408 nm and 532 nm laser excitation was evaluated as a diagnostic tool for oral mucosa discrimination in a clinical study with 150 subjects.

Conference End



A

Aalders, Maurice C. [8091-51]S10Aalto, Timo [8087-10]S3Aans, Jan Bonne [8087-15]S5Aaron, Jesse S. [8089-13]S3Abargues, Rafael [8092-51]SPS2Abbate, Francesca [8088-51]S10Abboud, Marie [8090-04]S1Abdat, Faiza [8087-57]S13Abderrafi, Kamal [8092-51]SPS2Abreu, Artur R. [8089-25]SPS1Acar, Handan [8087-08]S3Adriaenssens, Tom [8091-33]S7Adur, Javier F. [8086-20]S4Aglyamov, Salavat R. [8090-19]S4Agnarsson, Bjorn [8090-13]S3Agrba, Pavel D. [8091-64]SPS2Aguilar, Guillermo [8092-06]S1Ahmad Fadzil, M. Hani [8087-80]SAhn, Henrik [8087-48]S12Aivars, Juris [8090-30]SPS1Aizu, Yoshihisa [8088-24]S4Akarçay, Hidayet G. [8088-19]S4Akca, Imran B. [8091-25]S6Akens, Margarete K. [8089-14]S3Aladin, Mani [8092-56]SPS2Alahyane, Fatima [8092-35]S7,

[8092-44]S8Albanese, Alessio [8092-30]S6Ale, Angelique B. F. [8089-10]S2,

[8089-19]SPS1, [8089-22]SPS1Alex, Aneesh [8091-02]S1Alexandrou, Antigoni [8089-17]S3Alexandrov, Yuriy [8087-41]S9Alfieri, Domenico [8087-43]S10Allain, Jean-Marc [8086-42]SPS1Allier, Cedric P. [8086-44]SPS1Almeida, Diogo B. [8086-20]S4Almeida, Janete D. [8087-35]S8Almeida, Joana L. [8092-12]S3Alonso-Caneiro, David [8091-48]S9Alrubaiee, Mohammad [8088-33]S6Alves, Mônica [8087-35]S8Al-Wafi, Reem O. [8091-66]SPS2Amadei Nicolau, Renata [8092-57]

SPS2Amat-Roldán, Ivan [8086-21]S4Amelink, Arjen [8087-15]S5, 8090

ProgComm, 8090 S3 SessChrAnand, Arun [8086-59]SAndersen, Peter E. SympChair,

8087 SPLN SessChr, 8088 SPLN SessChr, 8091 SPLN SessChr, 8091 SJS1 SessChr, EB108 Chr, EBO11PLN S SessChr

Andersson-Engels, Stefan 8092 S2 SessChr, 8092 S3 SessChr, 8092 ProgComm

Andilla, Jordi [8086-02]S1Andrade, Liliana [8086-20]S4Andree, Stefan [8087-39]S9, [8087-

83]SAndreyev, Sergey V. [8087-75]SAngelidou, Myria [8089-06]S1Anne, Marie-Laure [8087-100]SAnsari, Rehman [8091-78]SAntoine, Martine [8091-37]S8Applegate, Brian E. [8086-48]SPS1,

[8090-47]SJS2Aptel, Florent [8092-44]S8Araujo-Moreira, Fernando M. [8092-

46]SPS2Arce-Diego, José L. [8088-64]SPS1,

[8092-03]S1Argba, Pavel D. [8091-20]S5Arifler, Dizem [8087-89]SArnaut, Luis G. [8089-25]SPS1Arnold, Thomas [8087-34]S8Arridge, Simon R. [8086-13]S3,

[8088-26]S5Arslanov, Denis D. [8087-06]S2Artigas, David [8086-02]S1, [8086-21]

S4, [8092-19]S4Asare, Lasma [8087-85]SAsproudis, Ioannis [8092-36]S7Assayag, Osnath [8091-37]S8Atlan, Michael [8090-02]S1, [8090-

04]S1

Audi, Said [8087-07]S3Aviles-Espinosa, Rodrigo A. [8086-

02]S1, [8092-19]S4Awazu, Kunio [8092-05]S1, [8092-47]

SPS2Ayas, Sencer [8087-08]S3Azevedo, Luciane H. [8087-59]S13

B

Baade, Alex [8092-37]S7, [8092-39]S7, [8092-27]S6

Bacak, Irena [8092-32]S6Bacharis, Constantine [8092-36]S7Baets, Roel [8091-26]S6Baeumler, Wolfgang 8092

ProgComm, 8092 S4 SessChrBagnaninchi, Pierre O. [8086-25]S5Bagnato, Vanderlei S. [8087-02]S1,

8090 ProgComm, [8092-08]S3Bahgat Shehata, Andrea [8090-27]

SPS1Bakshaeva, Ekaterina A. [8091-64]

SPS2Bal, Ufuk [8086-50]SPS1Balalaeva, Irina V. [8089-23]SPS1Baldini, Francesco 8087 S13

SessChr, [8087-47]S12, [8087-65]S

Balduzzi, Donatella [8086-12]S2, [8089-11]S2

Balestreri, Nicola [8088-51]S10Balu, Mihaela [8092-14]S4Barbarin, Yohan [8092-19]S4Barbastathis, George [8086-11]S2Baretton, Gustavo [8087-54]S13Bargigia, Ilaria [8088-43]S9, [8090-

27]SPS1Barriere, Clement [8087-92]SBartelt, Hartmut [8087-64]S, [8087-

84]SBarton, Jennifer K. 8091 ProgCommBarwari, Kurdo [8091-40]S8Baselli, Giuseppe [8088-01]S1Basevi, Hector [8088-56]SPS1Bassi, Andrea [8087-53]S13, [8090-

27]SPS1Batista, Nídia P. [8092-49]SPS2Baum, Kirstin [8088-68]SBauman, Brian J. [8091-46]S9Baumann, Bernhard [8091-07]S3,

[8091-15]S4, [8091-19]S5Bazant-Hegemark, Florian [8091-73]

SPS2Beard, Paul C. 8090 ProgCommBeaurepaire, Emmanuel 8086 Chr,

8086 S4 SessChr, 8086 SPS1 SessChr, [8086-19]S4, [8086-24]S5, [8086-34]S7, [8090-45]SJS2

Beckmann, Martin F. [8088-40]S8Been, Stefan [8092-56]SPS2Beer, Sebastian [8086-45]SPS1,

[8086-57]SPS1Bellas, Evangelia [8086-05]S1Bellemain, Alain [8086-42]SPS1Bellon-Fontaine, Marie-Noëlle [8087-

09]S3Belyaev, Andrey V. [8087-75]SBendsoe, Niels 8087 ProgCommBennett, Eric [8086-52]SPS1Benoit, Emilie [8088-38]S8Berclaz, Corinne [8091-38]S8Berer, Thomas [8090-34]SPS1Berger, Andrew J. 8087 ProgCommBerger, Joern [8087-32]S8Berger, Michel [8088-46]S9Bergmann, Florian [8092-10]S3Bergmann, Thorsten [8086-45]SPS1,

[8086-57]SPS1Bergner, Gero [8087-46]S11, [8087-

64]SBergner, Norbert [8087-30]S8Bernier, Damien [8087-10]S3Bérubé-Lauzière, Yves [8088-21]S4,

[8088-27]S5Beuf, Olivier [8087-21]S6Beuthan, Jürgen [8088-35]S6

Bever, Marco [8092-37]S7, [8092-39]S7

Bezerra, Hiram G. [8091-34]S7Bhattacharya, Kunal [8087-12]S4Bhuvaneswari, Ramaswamy [8086-

31]S6, [8090-09]S2Bianchi, Anna M. [8088-01]S1Biedermann, Benjamin R. [8091-08]

S3, [8091-24]S6, [8091-55]SPS2Bilyi, Oleksandr I. [8087-76]S, [8086-

55]SPS1Bilyy, Rostyslav O. [8087-69]SBinder, Susanne [8091-45]S9Birk, Udo J. [8092-27]S6Birngruber, Reginald [8092-37]S7,

[8092-38]S7, [8092-39]S7Bisaillon, Charles-Etienne [8091-36]

S7Bisker, Gili [8092-20]S4, [8092-43]S8Blaschke, Sabine [8088-35]S6Blatter, Cedric [8091-01]S1Blé, François X. [8092-13]S4Bloch, Wilhelm [8087-67]SBloise, Nora [8092-22]S5Blondel, Walter C. P. M. [8087-88]S,

[8092-17]S4Bluss, Kristaps [8090-26]SPS1Boas, David A. [8088-08]S2Boccara, A. Claude [8091-35]S7,

8086 ProgComm, [8091-37]S8Bocchio, Noelia [8090-15]S3Bock, Rüdiger [8091-19]S5Bocklitz, Thomas [8087-04]S1,

[8087-16]S5Böhler, Christof [8092-40]S8Boilot, Jean-Pierre [8089-17]S3Bolzoni, Luca [8087-65]SBonesi, Marco [8091-08]S3, [8091-

09]S3, [8091-13]S4Bonin, Tim [8091-50]S10, [8091-53]

S10Bonnier, Franck 8087 S5 SessChr,

[8087-12]S4Bonwick, Bob [8091-02]S1Boppart, Stephen A. 8087 S3

SessChr, 8087 S2 SessChr, [8087-01]S1, 8091 ProgComm

Bornitz, Matthias [8091-31]S7Boschke, Elke [8087-63]SBoss, Daniel [8086-07]S2Bosschaart, Nienke [8091-51]S10Bossecker, Anja [8087-05]S2Bossy, Emmanuel [8088-38]S8Böttcher, Ingrid [8091-45]S9Bottcher-Luiz, Fatima [8086-20]S4Bouchard, Matthew B. [8086-29]S6Boudoux, Caroline [8086-24]S5Bouma, Brett E. 8091 Chr, 8091 S7

SessChr, [8091-32]S7Bourgine, Paul [8086-34]S7Bourguignon, Bernard [8087-09]S3Bou-Saïd, Benyebka [8087-21]S6Bousi, Evgenia [8091-22]S5Boussard-Pledel, Catherine [8087-

23]S6, [8087-100]SBouvard, Sandrine [8087-21]S6Bouwens, Arno [8091-04]S2, [8091-

38]S8Bouza-Domínguez, Jorge [8088-21]

S4, [8088-27]S5Bouzigues, Cédric [8089-17]S3Bradu, Adrian [8091-30]S6, [8091-68]

SPS2Bragagna, Thomas [8092-40]S8Bragheri, Francesca [8092-22]S5Brambilla, Marco [8088-31]S6Branchini, Lauren [8091-19]S5Brandily, Marie-Laure [8087-23]S6Brehm, Bernhard [8087-46]S11Brenchley, Paul [8091-66]SPS2Brenner, Carsten [8088-40]S8Breunig, Hans Georg [8087-41]S9,

[8092-14]S4Briandet, Romain [8087-09]S3,

[8087-52]S13Brice, Tamara [8090-26]SPS1Brinkmann, Ralf 8092 ProgComm,

8092 S7 SessChr, 8092 S1 SessChr, [8092-27]S6, [8092-29]S6, [8092-37]S7, [8092-38]S7, [8092-39]S7

Brown, J. Quincy 8087 S11 SessChr, [8087-17]S5, [8087-19]S6

Bruch, Hans-Peter [8092-29]S6Bruehl, Ruediger [8088-03]S1, [8088-

02]S1Bruns, Thomas [8089-12]S2Bruscino, Nicola [8087-43]S10Bruyninckx, Vincent [8092-56]SPS2,

[8092-58]SPS2Buchinger, Birgit [8087-96]SBuckley, Erin M. [8088-10]S2Buehler, Andreas [8089-02]S1,

[8089-26]SPS1Bugeon, Laurence [8086-13]S3Bulard, Emilie [8087-09]S3Burcheri, Adriano [8091-37]S8,

[8092-68]S2Bureau, Bruno [8087-23]S6, [8087-

100]SBurg, Jan Michael [8086-45]SPS1Burgholzer, Peter [8090-34]SPS1,

[8090-35]SPS1Burgner, Jessica [8092-34]S6Burgoyne, Bryan [8091-29]S6Burkhardt, Anke [8091-31]S7Burock, Susen [8088-52]S10Buschmann, Volker [8086-15]S3Bydlon, Torre M. [8087-19]S6Bykov, Alexander [8090-28]SPS1Bykov, Alexander V. [8091-60]SPS2Byrne, Hugh J. [8087-12]S4

C

Caduff, Andreas [8087-74]SCaffini, Matteo [8088-01]S1, [8088-

08]S2, [8088-14]S3Cai, Wei [8088-33]S6Callow, Jim A. [8091-74]SPS2Callow, Maureen E. [8091-74]SPS2Camacho-Lopez, Santiago [8092-06]

S1, 8092 ProgCommCampagnola, Paul J. 8086

ProgCommCampbell, Gordon [8091-36]S7Campolmi, Piero [8087-44]S10Candiani, Gabriele [8087-53]S13Canva, Michael T. [8086-42]SPS1Capps, Arlie G. [8091-46]S9Caron, Serge [8090-16]S3Carrasco-Zevallos, Oscar [8086-48]

SPS1Carvalho, Luis Felipe C. [8087-35]S8Casadessus, Olivier [8091-49]S9Casellas, Oscar [8088-42]S8Cassano, Enrico [8088-51]S10Castro-e-Silva, Orlando [8087-02]S1Cauberg, Evelyne C. [8091-40]S8Cencic, Boris [8092-32]S6Cense, Barry [8091-57]SPS2Cerbai, Elisabetta [8086-18]S4Cerutti, Sergio [8088-01]S1Chabrier, Renée [8092-13]S4Chan, Robert K. Y. [8087-61]SChandra, Malavika [8087-36]S8Charalambous, Ismini [8091-22]S5Charbon, Edoardo [8088-37]S7Chauvet, Laurence [8087-62]SChelak, Vyacheslav N. [8087-75]SChen, Chung-Ming [8087-27]S7Chen, Juan [8089-14]S3, [8089-21]

SPS1Chen, Leng-Chun [8087-31]S8,

[8087-36]S8, [8089-18]S3, [8090-14]S3

Chen, Lingling [8086-13]S3Chen, Nanguang 8086 S6 SessChr,

[8086-17]S3, [8086-36]SPS1, [8088-65]S, [8091-12]S3

Chen, Thomas [8089-05]S1Cheng, Kuen Chiuan [8086-53]SPS1Chhaniwal, Vani K. [8092-67]S2Childs, David T. D. [8091-61]SPS2Chiu, Han-Mo [8091-69]SPS2Choi, Heejin [8086-58]SPS1Choi, John M. [8086-35]S7Chong, Shau Poh [8086-17]S3,

[8086-36]SPS1

Index of Authors, Chairs, and Committee Members


Chu, Anthony [8087-41]S9Chuang, Ching-Cheng [8087-27]S7,

[8091-43]S8Chuang, Frank 8092 ProgCommChyad, Radhi M. [8090-32]SPS1Cibas, Edmund [8087-24]S6Cicchi, Riccardo [8087-43]S10,

[8087-44]S10Cichon, Günter [8087-94]SCifuentes, Jose C. [8088-42]S8Cihan, Can [8087-89]SCimalla, Peter [8091-31]S7, [8091-41]

S8, [8091-56]SPS2, [8091-58]SPS2

Clancy, Neil T. [8090-01]S1Clays, Koen [8086-46]SPS1Claytor, Kevin [8086-23]S5Clement, J. H. [8087-16]S5Clément, Olivier [8089-17]S3Cleret, Aurélie [8086-43]SPS1Clevenger, Randall [8089-24]SPS1Coda, Sergio [8087-25]S6Connelly, Michael J. [8091-70]SPS2Connelly, Patricia [8089-24]SPS1Connors, Daniel P. [8092-31]S6Conrath, John [8091-49]S9Contini, Davide [8088-01]S1, [8088-

08]S2, [8088-09]S2, [8088-12]S2, [8088-14]S3, [8088-57]SPS1

Cook, Tim [8087-22]S6Coosemans, Mark [8091-33]S7Coppini, Raffaele [8086-18]S4Coppola, Giuseppe [8089-11]S2Correa, Rodrigo B. [8087-02]S1Correia, Carlos M. [8086-54]SPS1Costa, Marco A. [8091-34]S7Cote, Daniel 8090 ProgCommCotte, Yann J. [8086-10]S2Couderc, Vincent [8086-26]S5,

[8087-62]SCoutard, Jean Guillaume [8086-44]

SPS1, [8088-31]S6Cremien, Didier [8087-62]SCrépeau, Joël [8092-62]S2Cretaio, Erica [8090-41]SPS1Cristescu, Simona M. [8087-06]S2,

[8087-55]S13Cristiani, Ilaria [8092-22]S5Croteau, Andre [8090-16]S3Crotti, Caroline [8092-35]S7, [8092-

44]S8Cubeddu, Rinaldo [8088-01]S1,

[8088-08]S2, [8088-12]S2, [8088-14]S3, [8088-43]S9, [8088-51]S10

Cuccia, David J. [8087-18]S5Curatolo, Andrea [8091-21]S5, [8091-

27]S6

D

da Silva, Anabela [8088-44]S9, [8088-46]S9

da Silva Martinho, Herculano [8087-35]S8

da Silva Ribeiro, Elizeu [8092-61]SPS2

Daddi Qubekka, Samia [8087-52]S13Dalla Mora, Alberto [8088-09]S2,

[8090-27]SPS1Dallman, Margaret J. [8086-13]S3Daly, Susan M. [8091-72]SPS2Dana, Aykutlu [8087-08]S3D’Andrea, Cosimo [8087-53]S13Danicke, Veit [8092-29]S6Daniels, Mathew [8089-24]SPS1Das, Bhargab [8090-05]S1Dasari, Ramachandra R. [8086-09]S2Davis, Scott [8088-36]S7, [8089-09]

S2Dawczynski, Jens [8087-50]S12Dayal, Rajeev [8088-54]S10de Araujo, Maria Tereza [8092-54]

SPS2, [8092-59]SPS2De Biasio, Martin [8087-34]S8de Boer, Johannes F. [8086-28]

S6, 8091 ProgComm, 8091 S3 SessChr

de Bruin, Daniel M. [8091-40]S8, [8091-42]S8

de Groot, Mattijs [8086-28]S6de la Rosette, Jean J. [8091-40]S8De La Rue, Richard M. [8087-10]S3De Martino, Antonello [8087-104]SDe Meulenaere, Evelien [8086-46]

SPS1de Oliveira, Marco Antonio [8092-57]

SPS2De Poly, Bertrand [8091-37]S8de Ridder, René M. [8091-25]S6de Rijke, Theo [8091-40]S8de Roode, Rowland [8087-28]S7de Thomaz, Andre A. [8086-20]S4Deán-Ben, Xosé L. [8090-36]SPS1,

[8090-37]SPS1, [8090-42]SPS1Débarre, Delphine [8086-34]S7Deckert, Volker 8087 ProgCommDehghani, Hamid 8088 ProgComm,

8088 S3 SessChr, 8088 S4 SessChr, 8088 S6 SessChr, [8088-36]S7, [8088-56]SPS1

Delgado-Mederos, Raquel [8088-04]S1

Deliolanis, Nikolaos C. 8089 ProgComm, [8089-26]SPS1

Della Frera, Adriano [8090-27]SPS1Dellagiacoma, Claudio [8086-14]S3Deloison, Florent [8092-44]S8Delon, Antoine [8086-27]S5Denisov, Nikolay N. [8092-04]S1Depeursinge, Christian D. 8086

ProgComm, [8086-07]S2, [8086-08]S2, [8086-10]S2, [8090-03]S1

Derenko, Susan [8087-54]S13Derjabo, Alexander [8087-87]SDesmet, Walter [8091-33]S7Detre, John A. [8088-10]S2Deumié, Carole [8088-46]S9, [8091-

49]S9Deutsch, Lydia [8087-50]S12Dhadwal, Gurbir [8090-44]SPS1Dholakia, Kishan 8086 ProgComm,

8086 SJS2 SessChr, 8087 ProgComm, 8090 SJS2 SessChr

D’hooge, Jan [8091-33]S7Di Caprio, Giuseppe [8089-11]S2Di Cicco, Emiliano [8092-55]SPS2Di Ninni, Paola [8088-12]S2Diamantino, Alexandre Greca [8092-

57]SPS2Diao, Diana [8090-44]SPS1Dickinson, Mark R. [8091-66]SPS2Diebele, Ilze [8087-87]SDietzek, Benjamin [8087-30]S8,

[8087-46]S11, [8087-64]S, [8087-84]S

diFlorio-Alexander, Roberta [8089-09]S2

Digman, Michelle A. [8086-03]S1Ding, Siqi [8090-43]SPS1Dinia, Lavinia [8088-04]S1Dinten, Jean Marc [8086-44]SPS1,

[8088-31]S6, [8088-46]S9do Espirito Santo, Ana Maria M.

[8092-57]SPS2Dobre, George M. [8091-68]SPS2Dochow, S. [8087-16]S5, [8087-84]SDong, Chen-Yuan [8086-41]SPS1,

[8087-33]S8, [8092-50]SPS2Donner, Sabine [8086-22]S4Donohue, Danielle [8089-24]SPS1Dorney, Jennifer [8087-12]S4Doroshenko, Dgaser [8091-44]S8Dortu, Fabian [8087-10]S3Douady, Julien [8086-43]SPS1Douplik, Alexandre [8087-91]S,

[8087-95]SDownes, Andrew R. [8086-25]S5Dressler, Cathrin [8087-94]SDrexler, Wolfgang [8087-501]

SPLN, 8091 S9 SessChr, 8091 ProgComm, [8091-02]S1, [8091-45]S9

Dreyer, Thiago R. [8092-23]S5Drysdale, Robert [8088-67]SDubois, Christophe [8091-33]S7Dubolazov, Alexander V. [8087-99]S

Dubost, Henri [8087-09]S3Dufour, Marc L. [8091-36]S7Duker, Jay S. [8091-15]S4, [8091-19]

S5Duloquin, Louise [8086-34]S7Dunsby, Chris [8087-25]S6, [8086-

30]S6, [8087-41]S9Duong, Jennifer [8086-03]S1Durduran, Turgut 8088 ProgComm,

8088 S8 SessChr, 8088 S10 SessChr, [8088-04]S1, [8088-10]S2, [8088-25]S5, [8088-41]S8, [8088-42]S8

Dziekan, Thomas [8087-32]S8

E

Ebert, Bernd [8087-32]S8, [8088-09]S2

Ebrahim-Zadeh, Majid [8092-56]SPS2

Egger, Holger [8087-10]S3Egner, Alexander [8092-65]S2Eichler, Hans Joachim [8087-94]SEiden, Stefanie [8087-10]S3Eigenwillig, Christoph M. [8091-08]

S3, [8091-24]S6, [8091-55]SPS2Ekiz, Okan O. [8087-08]S3Elagin, Vadim V. [8092-04]S1Elfick, Alistair P. D. [8086-25]S5Eliceiri, Kevin W. 8086 ProgCommElliott, Jonathan T. [8088-55]SPS1Elson, Daniel S. [8087-25]S6, [8087-

92]S, [8090-01]S1Emelianov, Stanislav Y. [8089-13]S3,

8090 ProgComm, [8090-19]S4Engel, Guy [8086-32]S6Engelhardt, Ralph [8091-45]S9Englmeier, Karl Hans [8089-20]SPS1Enrichi, Francesco [8090-41]SPS1Erdmann, Rainer [8086-15]S3Eriksen, Eric F. [8087-96]SErmolayev, Vladimir [8089-10]S2,

[8089-19]SPS1Erry, Gavin R. G. [8091-73]SPS2Ertmer, Wolfgang A. [8092-24]S5Esmaeelpour, Marieh [8091-45]S9Esposito, Giuseppe [8092-30]S6Esteban-Martin, Adolfo [8092-56]

SPS2Estracanholli, Éverton S. [8090-33]

SPS1

F

Faber, Dirk J. [8087-13]S4, 8090 ProgComm, [8091-40]S8, [8091-42]S8, [8091-51]S10, [8091-52]S10

Fanjul-Vélez, Félix [8088-64]SPS1, [8092-03]S1

Farahi, Salma [8088-38]S8, [8088-39]S8

Farina, Andrea [8088-43]S9, [8090-27]SPS1

Farrell, Thomas J. [8092-09]S3Farzam, Parisa [8088-41]S8, [8088-

42]S8Faure, Emmanuel [8086-34]S7Fedosejevs, Robert 8092 ProgCommFehre, Jens [8087-54]S13Feinberg, Stephen E. [8089-18]S3,

[8090-14]S3Felbermayer, Karoline [8090-34]SPS1Felsenstein, Jerome M. [8092-31]S6Ferensovich, Yaroslav P. [8086-55]

SPS1Ferrantini, Cecilia [8086-18]S4Ferraro, Pietro [8086-12]S2, [8089-

11]S2Ferreira, Juliana [8087-02]S1, [8092-

08]S3Fiebich, Martin [8086-45]SPS1,

[8086-57]SPS1

Fiks, Ilya I. [8089-23]SPS1Filippidis, George [8092-19]S4Finizio, Andrea [8086-12]S2, [8089-

11]S2Fiole, Daniel [8086-43]SPS1Fiorito, Pablo A. [8092-23]S5Firdous, Shamaraz [8090-50]SFischer, Martin C. [8086-23]S5, 8087

S9 SessChr, [8087-42]S10Fontaine-Aupart, Marie-Pierre [8087-

09]S3, [8087-52]S13Fontana, Carla R. [8092-11]S3Fortuna, Damiano [8092-55]SPS2Foschum, Florian [8088-59]SPS1Fourligas, Nikolaos [8086-05]S1Frampton, Jon [8088-56]SPS1Franceschetti, Silvana [8088-01]S1Francisco, Ana L. [8087-59]S13Fraser, Scott E. [8086-35]S7Fredriksson, Ingemar [8087-56]S13Freiberger, Manuel [8088-62]SPS1,

[8088-63]SPS1French, Paul M. 8086 ProgComm,

[8086-13]S3, [8087-25]S6, [8087-41]S9

Frenz, Martin 8092 ProgComm, 8092 S1 SessChr

Friedrich, Claus-Stefan [8088-40]S8Fritz, Andreas [8092-38]S7Fu, Chit Yaw [8090-09]S2, [8090-11]

S2Fu, Henry L. [8087-19]S6Fuertes, Gustavo [8092-51]SPS2Fugger, Oliver [8088-23]S4Fujimoto, James G. 8091

ProgComm, [8091-15]S4, [8091-19]S5, [8091-501]SJS1

Fukuda, Keiko [8088-49]S9Funk, Richard H. W. [8087-49]S12Furjes, Peter [8087-10]S3Füssel, Susanne [8087-54]S13

G

Gacoin, Thierry [8089-17]S3Gaertner, Maria [8091-41]S8, [8091-

56]SPS2Galbrun, Ernest [8092-17]S4Galland, Rémi [8086-27]S5Galli, Andrea [8086-12]S2, [8089-11]

S2Gallimore, Gary S. [8091-71]SPS2Gamm, Ute [8087-15]S5Gamsjaeger, Sonja [8087-96]SGao, Guangjun [8086-17]S3, [8086-

36]SPS1Gao, Jie [8086-27]S5Gao, Yuan [8087-37]S8García-Allende, Pilar Beatriz [8087-

92]SGarraud, Kévin [8086-43]SPS1Gärtner, Maria [8091-58]SPS2Gasser, Juerg A. [8087-96]SGayen, Swapan K. [8088-33]S6Geissbuehler, Matthias [8090-15]S3Geissbühler, Stefan [8086-14]S3Gelikonov, Grigory V. [8091-44]S8,

[8091-67]SPS2, [8091-75]SPS2Gelikonov, Valentin V. [8091-44]S8,

[8091-67]SPS2Gemeinhardt, Ines [8087-32]S8Georgakoudi, Irene SympChair, 8086

ProgComm, 8086 SJS2 SessChr, 8086 S3 SessChr, [8086-05]S1, 8087 SPLN SessChr, [8087-24]S6, 8088 SPLN SessChr, 8090 SJS2 SessChr, 8091 SPLN SessChr, 8092 S2 SessChr, EB108 Chr, EBO11PLN S SessChr

Georges, Gaëlle [8091-49]S9Geradts, Joseph [8087-17]S5, [8087-

19]S6Gerega, Anna [8088-45]S9Gerhardt, Nils C. [8088-40]S8, [8091-

65]SPS2



Gerich, Carola E. [8087-54]S13Gerritsen, Hans C. [8087-14]S4Gersonde, Ingo [8087-39]S9, [8087-

83]S, [8087-94]S, [8088-60]SPS1Getman, Vasyl B. [8086-55]SPS1,

[8087-76]SGhadyani, Hamid R. [8088-36]S7,

[8088-66]SGhini, Giacomo [8087-47]S12Giannetti, Ambra [8087-47]S12,

[8087-65]SGiannone, Domenico [8087-10]S3Giannoula, Alexia [8088-25]S5Giglioli, Isabella [8088-01]S1Gillispie, Gregory D. [8087-31]S8Gioffrè, Mariano [8089-11]S2Gisquet, Héloïse [8087-88]SGiusti, Jucaira S. [8092-11]S3Glatz, Jürgen [8089-26]SPS1Glückstad, Jesper 8086 ProgCommGnoerrlich, Tim [8088-52]S10Goh, Wenda [8090-11]S2Golan, Lior [8092-43]S8Golbin, Denis [8087-29]S7Gonçalves, Daniela [8092-11]S3Gong, Longjing [8087-37]S8Gopinath, Subash C. B. [8086-40]

SPS1Götzinger, Erich [8091-07]S3, [8091-

08]S3, [8091-09]S3, [8091-13]S4Goulley, Joan [8091-38]S8Gousha, Artyom [8087-29]S7Grabar, Alexander A. [8088-39]S8Grabovskis, Andris [8090-29]SPS1,

[8090-30]SPS1Grachev, Pavel N. [8087-29]S7Grad, Ladislav [8092-32]S6Graf, Rudolf [8088-07]S2Grajciar, Branislav [8091-01]S1Gramer, Markus [8088-07]S2Grapin-Botton, Anne [8091-38]S8Gratt, Sibylle [8090-20]S4, [8090-35]

SPS1Gratton, Enrico [8086-03]S1Grecco, Clóvis [8092-08]S3, [8092-

53]SPS2Greenberg, Joel H. [8088-10]S2Grieger, Svenja [8088-53]S10Grimm, Jan 8089 ProgCommGrimstad, Øystein [8087-40]S9Grosberg, Lauren [8086-01]S1Grosenick, Dirk [8088-52]S10Gross, Michel [8090-04]S1Gross, Simon [8092-40]S8Grün, Hubert [8090-34]SPS1Grunze, Michael [8091-74]SPS2Guggenheim, James A. [8088-56]

SPS1Guillemin, François H. [8087-88]SGuillet de Chatellus, Hugues [8088-

11]S2Guler, Mustafa O. [8087-08]S3Gulvady, Ranjit [8086-42]SPS1Guner, Hasan [8087-08]S3Gurevich, Boris S. [8087-75]SGusachenko, Ivan [8086-19]S4Guvener, Nihan [8087-08]S3

H

Haatainen, Tomi [8087-10]S3Habermehl, Christina [8088-17]S3Hadjigeorgiou, Katerina [8087-03]S1Hagen, Axel J. [8088-52]S10Hagen, Clemens [8092-40]S8Hah, Hoe Jin [8089-05]S1Haisch, Christoph 8092 ProgComm,

8092 S8 SessChrHaist, Tobias [8086-49]SPS1Hajiaboli, Amir [8089-04]S1Hajizadeh, Faeghe [8092-02]S1Hajjarian, Zeinab [8090-07]S1Hajnal, Jo V. [8086-13]S3Halidi, Nadia [8091-04]S2Häme, Yrjö [8088-35]S6Hamilton, Craig [8092-19]S4Hammer, Martin [8086-04]S1, [8087-

50]S12

Han, Tae-Young [8087-54]S13Hance, Dalton [8088-10]S2Hansen, Anja [8092-42]S8Harmany, Zachary T. [8087-17]S5Harmelin, Alon [8090-06]S1Harms, Fabrice [8091-37]S8Harren, Frans J. M. [8087-06]S2,

[8087-55]S13Härtel, Sascha [8088-53]S10Härtling, Thomas [8087-54]S13Hartsuiker, Liesbeth [8087-58]S13Hasler, Malte [8086-49]SPS1Hasnain, Ali [8088-65]SHaug, Ingvild J. [8087-40]S9Heide, Michael [8092-10]S3Heijblom, Michelle [8087-20]S6,

[8090-21]S4Heine, Angela [8088-02]S1, [8088-03]

S1Heine, Marie-Theres [8088-59]SPS1,

[8092-18]S4Heinrich, Arne [8092-40]S8Heisterkamp, Alexander [8086-22]

S4, [8092-24]S5, [8092-42]S8, [8092-45]S8

Helfmann, Jürgen [8087-39]S9, [8087-83]S, [8087-94]S

Hell, Stefan W. 8086 ProgCommHendaoui, Nordine [8092-56]SPS2Henderson, Robert K. [8086-27]S5Henkel, Thomas [8087-64]SHernandez-Palacios, Julio E. [8087-

40]S9Herry, Jean-Marie [8087-09]S3Hervé, Lionel [8088-31]S6Herzig, Hans Peter [8090-12]S2Herzog, Eva [8089-10]S2, [8090-22]

S4Heverhagen, Johannes T. [8086-45]

SPS1, [8086-57]SPS1Heysselaer, Daniel [8092-58]SPS2Hibst, Raimund 8092 ProgComm,

8092 S4 SessChrHielscher, Andreas H. 8088 SPS1

SessChr, 8088 S7 SessChr, 8088 S4 SessChr, 8088 S5 SessChr, 8088 Chr, [8088-22]S4, [8088-35]S6, [8088-54]S10

Hillman, Elizabeth M. C. 8086 S7 SessChr, [8086-01]S1, [8086-29]S6

Hillman, Timothy R. [8091-21]S5Hillmann, Dierck [8091-50]S10Hintermüller, Michael [8088-63]SPS1Hirao, Tetsuji [8088-24]S4Hitzenberger, Christoph K.

SympChair, [8091-07]S3, [8091-08]S3, [8091-09]S3, [8091-13]S4

Hnatush, Svitlana O. [8087-76]SHo, Yi-Ching [8091-43]S8Hofer, Bernd [8091-02]S1, [8091-45]

S9Hoffart, Louis [8091-49]S9Hoffman, Robert M. [8086-03]S1Hofmann, Martin R. [8088-40]S8,

[8091-65]SPS2Hogg, Richard A. [8091-61]SPS2Hohmann, Ansgar [8090-17]S3Hohmann, Martin [8087-95]SHollmach, Julia [8087-49]S12Holtze, Susanne [8088-17]S3Honda, Norihiro [8092-47]SPS2Hong, Young-Joo [8091-14]S4Hornegger, Joachim [8091-15]S4,

[8091-19]S5Horstmann, Jens [8092-27]S6Horvath, Robert [8087-10]S3Hovhannisyan, Vladimir A. [8086-41]

SPS1, [8087-33]S8Hoy, Paul R. [8087-90]SHoyng, Carel B. [8091-57]SPS2Hsieh, Yao-Sheng [8087-27]S7,

[8091-43]S8Hsueh, Chiu-Mei [8086-41]SPS1Hu, Sijung [8087-102]SHuang, David M. [8091-15]S4Huang, Shu-Wei [8091-69]SPS2Huang, Wei-Cheng [8091-69]SPS2Huang, Xiabing [8087-68]SHuang, Yu [8086-56]SPS1

Huber, Robert A. 8091 ProgComm, 8091 S6 SessChr, [8091-01]S1, [8091-08]S3, [8091-24]S6, [8091-55]SPS2

Huignard, Jean-Pierre [8088-39]S8Hunter, Martin [8087-24]S6Huss, Guillaume [8086-26]S5Hussain, Altaf [8090-38]SPS1, [8090-

43]SPS1Hüttmann, Gereon [8091-50]S10,

[8091-53]S10Hüttmann, Gereon [8092-01]S1

I

Igarashi, Takanori [8090-39]SPS1Ilev, Ilko K. [8090-08]S1Illing, Gerd [8087-94]S, [8088-60]

SPS1Imbriani, Marcello [8092-22]S5Inada, Natalia M. [8092-61]SPS2Intes, Xavier 8088 ProgComm, 8088

S8 SessChr, 8088 S9 SessChrInui, Asuka [8087-66]SIonita, Ciprian [8091-62]SPS2Ionita, Iulian G. [8092-25]S5, [8092-

41]S8Ishihara, Miya [8087-97]SIshii, Katsunori [8092-05]S1, [8092-

47]SPS2Ishimaru, Ichiro [8087-66]SIttermann, Bernd [8088-02]S1, [8088-

03]S1Ivascu, Ioana R. [8091-17]S4

J

Jacobs, Arthur [8088-02]S1Jacobs, James W. [8091-11]S3Jaedicke, Volker [8091-65]SPS2Jaffray, David A. [8090-16]S3Jagannath, Ravi Prasad K. [8088-29]

S5Jäger, Marion [8088-16]S3Jaillon, Franck [8091-47]S9Jakovels, Dainis [8087-82]SJansen, Krista [8090-18]S4Jeffries, Kenneth [8089-24]SPS1Jelzov, Alexander [8088-14]S3Jelzow, Alexander [8088-02]S1,

[8088-03]S1, [8088-09]S2, [8088-12]S2, [8088-57]SPS1

Jentsch, Susanne [8087-50]S12Jeremyn, Michael [8088-66]SJermyn, Michael [8088-36]S7Jetzfellner, Thomas [8089-20]SPS1Jezersek, Matija [8092-32]S6Jia, Jingfei [8088-35]S6Jiang, Shudong [8089-09]S2Jimenez Villar, Ernesto [8092-51]

SPS2Joffre, Manuel [8086-24]S5Johansson, Ann [8092-10]S3Johnson, Nigel P. [8087-10]S3Jolivot, Romuald [8087-80]SJonathan, Enock [8091-72]SPS2Jones, Steven M. [8091-46]S9Jörger, Manfred [8088-40]S8Jose, Jithin [8089-03]S1Joud, Fadwa [8090-04]S1Jourdain, Pascal [8090-03]S1Ju, Jau Jiu [8086-53]SPS1Ju, Myeong-Jin [8091-14]S4Junker, Marlee [8087-19]S6

K

Kacprzak, Michal [8088-12]S2, [8088-14]S3, [8088-15]S3, [8088-45]S9

Kaestner, Lars [8086-47]SPS1, [8089-16]S3

Kakoudgi, Ernest [8092-56]SPS2Kakuta, Hirokazu [8088-48]S9,

[8088-50]S9Kalchenko, Vyacheslav [8090-06]S1Kalff, Rolf [8087-30]S8Kalinina, Aleksandra [8090-30]SPS1Kalkman, Jeroen [8091-03]S1, [8091-

25]S6, [8091-52]S10Kaluzny, Bartlomiej J. [8091-48]S9Kamensky, Vladislav A. [8091-20]S5,

[8091-64]SPS2, [8092-04]S1Kamm, Roger D. [8086-11]S2Kanick, Stephen C. [8087-15]S5kanno, Iwao [8088-13]S2Kaplan, David L. [8086-05]S1Kapostinsh, Janis [8087-87]SKapsokalyvas, Dimitrios [8087-43]

S10, [8087-44]S10Karnowski, Karol M. [8091-48]S9Kasaragod, Deepa K. [8091-10]S3,

[8091-11]S3, [8091-61]SPS2Kasseck, Christoph [8091-65]SPS2Kastanos, Evdokia [8087-03]S1Kawaguchi, Hiroshi [8088-18]S3,

[8088-50]S9Kawauchi, Satoko [8087-97]SKeller, Ursula [8092-19]S4Kellnberger, Stephan [8089-04]S1Kemble, Camille [8086-52]SPS1Kemper, Björn [8086-06]S2Kendall, Catherine A. [8087-22]S6,

[8091-02]S1Kennedy, Brendan F. [8091-21]S5,

[8091-27]S6Kennedy, Gordon T. [8087-25]S6Kennedy, Stephanie A. [8087-19]S6Keshmiri, Hamid [8090-13]S3Ketelhut, Steffi [8086-06]S2Khalil, Michael A. [8088-54]S10Kho, Kiang-Wei [8090-11]S2Kiehntopf, Michael [8087-04]S1Kienle, Alwin [8086-38]SPS1, [8086-

39]SPS1, [8088-16]S3, [8088-20]S4, [8088-23]S4, [8088-59]SPS1, [8090-17]S3, [8092-18]S4

Kiessling, Fabian 8089 ProgCommKikuchi, Makoto [8087-97]SKim, Gwangseong [8089-05]S1Kim, Hyun K. [8088-54]S10, [8088-

22]S4, [8088-35]S6Kim, In-Kyong [8088-54]S10Kim, Meeri N. [8088-10]S2Kim, Seungsoo [8090-19]S4Kim, Youngjae [8091-29]S6Kirilina, Evgeniya P. [8088-02]S1,

[8088-03]S1Kirillin, Mikhail Y. [8091-20]S5, [8091-

64]SPS2Kirsch, David G. [8087-17]S5Kirsten, Lars [8091-41]S8Kiviniemi, Vesa [8090-31]SPS1Klaase, Joost [8087-20]S6Klaessens, John H. [8087-28]S7Klaushofer, Klaus [8087-96]SKleemann, Markus [8092-29]S6Klein, Thomas [8091-24]S6, [8091-

55]SPS2Klemm, Matthias [8087-50]S12Kleshnin, Mikhail S. [8089-23]SPS1Klevering, B. Jeroen [8091-57]SPS2Klose, Alexander D. [8088-30]S6Kloss, Sandra [8087-05]S2Kloster, Meike [8086-27]S5Knels, Lilla [8087-49]S12, [8091-58]

SPS2Knief, Peter [8087-12]S4Koberling, Felix [8086-15]S3Koch, Edmund [8087-49]S12, [8091-

18]S4, [8091-31]S7, [8091-39]S8, [8091-41]S8, [8091-56]SPS2, [8091-58]SPS2

Koch, Peter [8091-50]S10, [8091-53]S10

Koch, Stefan P. [8088-14]S3Kodach, Vitali M. [8091-52]S10Koenig, Anne [8088-31]S6Koenig, Karsten [8086-03]S1, [8087-

41]S9Kohl-Bareis, Matthias [8087-67]S,

[8087-68]S, [8088-07]S2, [8088-53]S10



Koinzer, Stefan [8092-37]S7Koishi, Kazuaki [8088-49]S9Kolari, Kai [8087-10]S3Kolesov, Ilya A. [8087-75]SKomorowska, Katarzyna [8091-26]S6König, Karsten 8086 ProgComm,

[8092-14]S4König, Marcelle [8086-15]S3Koning, Gerben [8089-03]S1Konovalov, Alexander B. [8088-28]S5Konovalov, Nikolay [8087-29]S7Koo, Yong-Eun L. [8089-05]S1Koos, David S. [8086-35]S7Kopelman, Raoul [8089-05]S1Köstinger, Alice [8088-62]SPS1,

[8088-63]SPS1Kotsifaki, Domna [8092-52]SPS2Kotsyumbas, Galyna I. [8087-76]SKotsyumbas, Ihor Y. [8086-55]SPS1Koulen, Peter [8090-25]SPS1, [8091-

71]SPS2Kowalczuk, Laura [8086-19]S4,

[8092-35]S7, [8092-44]S8Kowalczyk, Andrzej A. [8091-48]S9Kowalski, Luiz P. [8087-59]S13Kozlowski, Megan [8089-24]SPS1Krafft, Christoph [8087-16]S5, [8087-

46]S11, [8087-84]SKral, Andrej [8092-24]S5Krämer, Benedikt [8086-15]S3Krammer, Peter [8092-40]S8Krasieva, Tatjana [8092-14]S4Kraus, Martin F. [8091-15]S4, [8091-

19]S5Kray, Oya [8091-77]SKray, Stefan [8092-63]S2Kredzinski, Lukasz [8091-70]SPS2Krestnikov, Igor L. [8091-61]SPS2Krishnaswamy, Venkat [8088-36]S7,

[8087-18]S5, 8090 ProgCommKrstajic, Nik [8091-61]SPS2Krüger, Alexander [8086-22]S4Krüger, Jörg [8088-06]S2Kruizinga, Pieter [8090-19]S4Ksenofontov, Sergey [8091-44]S8Kuebler, Wolfgang [8091-58]SPS2Kuech, Thomas F. [8087-19]S6Kuehn, Jonas [8086-07]S2, [8090-03]

S1Kühn, Wolfgang [8087-94]SKumar, Penmetcha K. R. [8086-40]

SPS1Kumar, Sunil [8086-30]S6Kuo, Shiuhyang [8089-18]S3, [8090-

14]S3Kurachi, Cristina [8087-02]S1, [8087-

59]S13, [8090-33]SPS1, [8092-08]S3, [8092-11]S3, [8092-53]SPS2, [8092-54]SPS2, [8092-59]SPS2, [8092-60]SPS2, [8092-61]SPS2

Kurihara, Kazuki [8088-18]S3Kurunczi, Sandor [8087-10]S3Kusano, Masashi [8088-13]S2Kushnir, Ihor M. [8086-55]SPS1Kuszel, Jurii [8087-29]S7Kutzner, Claire [8088-53]S10Kuwahara, Tomohiro [8088-24]S4Kuzmin, Sergey G. [8087-29]S7Kuzmina, Ilona [8087-87]SKuznetsov, Yuri [8090-06]S1Kviesis-Kipge, Edgars [8087-85]S,

[8090-29]SPS1Kyriakides, Alexandros [8087-03]S1

L

Labroille, Guillaume [8086-24]S5Lacombe, Francois [8087-25]S6Lademann, Jürgen M. [8092-14]S4Laguna, M. [8091-40]S8Lahiri, Basudev [8087-10]S3Laine, Romain [8086-13]S3Lamard, Laurent [8092-58]SPS2Lamb, Jonathan R. [8086-13]S3Lamouche, Guy [8091-29]S6, [8091-

36]S7Lane, Kristin [8087-24]S6Lapina, Viktoria A. [8087-63]S

Larsson, Marcus [8087-48]S12, [8087-56]S13

Lasser, Theo [8086-14]S3, [8090-15]S3, 8091 ProgComm, 8091 S2 SessChr, [8091-04]S2, [8091-38]S8

Latka, Ines [8087-84]SLatour, Gaël [8086-19]S4Latrive, Anne [8091-35]S7Laughney, Ashley M. [8087-18]S5Laurain, Antoine [8088-63]SPS1Lavín-Castanedo, Ángela [8092-03]

S1Leahy, Martin J. 8087 S12 SessChr,

[8087-45]S11, [8091-72]SPS2Leblond, Frederic [8088-36]S7Ledroit, Sylvain [8087-62]SLee, Byeong-Ha [8091-14]S4Lee, Chia-Yen [8087-27]S7Lee, Hsuan-Shu [8092-50]SPS2Lee, Martin [8087-101]SLee, Sangdae [8087-54]S13Lee, Shyh-Yuan [8091-43]S8Lee, Tim K. [8090-44]SPS1Lee, Ting-Yim [8088-55]SPS1Lee, Yuan Chin [8086-53]SPS1Legeais, Jean-Marc [8092-44]S8Leitgeb, Rainer A. 8091 Chr, 8091 S5

SessChr, 8091 S1 SessChr, 8091 S SessChr, 8091 SPS2 SessChr, [8091-01]S1, [8091-07]S3, [8091-13]S4, [8091-16]S4, [8091-17]S4

Leitner, Raimund [8087-34]S8Lenarz, Thomas [8092-24]S5Lenferink, Aufried [8087-58]S13Lenz Cesar, Carlos [8086-20]S4Leosson, Kristjan [8090-13]S3Leproux, Philippe [8086-26]S5Leray, Aymeric [8092-66]S2Lesins, Janis [8087-86]S, [8092-15]

S4Levecq, Xavier [8086-02]S1L’Huillier, Jean-Pierre [8092-16]S4Li, Baolei [8086-23]S5Li, Feng-Chieh [8092-50]SPS2Li, Jianping [8087-61]SLi, Weitao [8087-98]SLi, Xiaozhou [8087-70]S, [8087-71]S,

[8087-72]S, [8087-78]S, [8087-79]S

Liang, Dawei [8092-12]S3, [8092-49]SPS2

Licha, Kai [8087-32]S8Liebert, Adam 8088 ProgComm,

8088 S10 SessChr, [8088-12]S2, [8088-14]S3, [8088-15]S3, [8088-45]S9, [8088-57]SPS1

Liemert, André [8088-20]S4Lilge, Lothar D. 8092 Chr, 8092 S3

SessChr, 8092 S5 SessChr, 8092 S1 SessChr, 8092 SPS2 SessChr

Lin, Charles P. 8086 ProgComm, 8089 Chr, 8089 SPS1 SessChr, [8089-08]S2

Lin, Chih-Ju [8092-50]SPS2Lin, Feng [8086-31]S6Lindbergh, Tobias [8087-48]S12Lines, Collin [8087-103]SLipinski, Hans-Gerd [8087-77]SLipp, Peter [8086-47]SPS1, [8089-16]

S3Lippok, Norman [8091-23]S5Liu, Baochang [8092-09]S3Liu, Honghui [8087-88]SLiu, Hongyuan [8087-68]SLiu, Jonathan J. [8091-15]S4, [8091-

19]S5Liu, Quan [8087-11]S3Liu, Tracy W. B. [8089-14]S3, [8089-

21]SPS1Liu, Xing [8087-98]SLloyd, William R. [8087-31]S8, [8087-

36]S8, [8089-18]S3, [8090-14]S3Lo, Justin Y. [8087-19]S6Lobo Ribeiro, Antonio B. [8091-30]S6Loew, Leslie M. [8086-18]S4Lombardi, Wellington [8092-61]SPS2Lopes Pinto, Clovis A. [8087-59]S13López-Escobar, María [8092-03]S1

Loreal, Olivier [8087-23]S6, [8087-100]S

Loschenov, Viktor B. [8087-29]S7Lotti, Jacopo [8086-18]S4Lotti, Torello [8087-43]S10, [8087-44]

S10Lowik, Clemens W. 8089 ProgCommLoza-Alvarez, Pablo [8086-02]S1,

[8086-21]S4, [8086-46]SPS1, [8092-19]S4

Lu, Chih-Wei [8091-69]SPS2Lu, Zenghai [8091-10]S3, [8091-11]

S3Lubatschowski, Holger [8092-45]S8Lucas, Jacques [8087-23]S6Luckmann, Heiko [8087-83]SLuengo-Oroz, Miguel A. [8086-34]S7Luft, Susanne [8092-39]S7Lührs, Christian [8091-50]S10Lui, Harvey [8090-44]SPS1Luo, Yuan [8086-11]S2Lusa, Vieda [8090-30]SPS1Lüttmann, Sven O. [8087-77]SLynch, Susanna K. [8086-52]SPS1Lyng, Fiona M. [8087-12]S4Lyon, Alexander R. [8086-30]S6Lyu, Hong Chou [8086-53]SPS1Lyubimov, Vladimir V. [8088-28]S5

M

Ma, Jun [8087-37]S8Macdonald, Rainer [8087-32]S8,

[8088-02]S1, [8088-03]S1, [8088-06]S2, [8088-09]S2, [8088-12]S2, [8088-52]S10, [8088-57]SPS1

Macedo, Milton P. [8086-54]SPS1MacLeod, Ken T. [8086-30]S6Madsen, Steen J. 8090 ProgCommMaeda, Saki [8086-37]SPS1Maeda, Takaaki [8088-24]S4Maeder, Ulf [8086-45]SPS1, [8086-

57]SPS1Magistretti, Pierre J. [8086-07]S2,

[8090-03]S1Magnain, Caroline V. [8090-02]S1Magnusson, Robert [8090-25]SPS1Magrini, Taciana D. [8092-23]S5Maira, Giulio [8092-30]S6Maitland, Kristen C. [8086-48]SPS1,

8087 ProgComm, [8092-64]S2Majauska, Madara [8090-30]SPS1Makita, Shuichi [8091-14]S4, [8091-

47]S9Makropoulou-Loukogiannaki, Mersini

I. [8092-21]S5, [8092-36]S7Malcolm, Graeme [8092-19]S4Mallidi, Srivalleesha [8089-13]S3Mandon, Julien [8087-55]S13Manetti, Leonardo [8092-55]SPS2Maniewski, Roman [8088-15]S3,

[8088-45]S9Manohar, Srirang [8087-20]S6,

[8087-58]S13, [8089-03]S1, [8090-21]S4

Mansouri, Chemseddine [8092-17]S4Mao, Weizheng [8087-37]S8Marcauteanu, Corina [8091-62]SPS2,

[8091-68]SPS2Marcelo, Cynthia L. [8089-18]S3,

[8090-14]S3Marciano, Tal [8092-35]S7, [8092-44]

S8Marcinkevics, Zbignevs [8090-30]

SPS1Mari, Meropi [8086-51]SPS1Märki, Iwan [8090-15]S3Marquet, Pierre P. [8086-07]S2,

[8086-08]S2, [8090-03]S1Marro Sánchez, Mónica [8087-51]

S12Martelli, Fabrizio [8088-12]S2Martí-Fàbregas, Joan [8088-04]S1Martínez-Pastor, Juan [8092-51]

SPS2Martinho, Herculano [8092-23]S5

Martins da Costa, Mardoqueu [8092-61]SPS2

Martin-Williams, Erica [8091-38]S8Marx, Ulrich [8091-59]SPS2März, Anne [8087-04]S1Marzani, Franck [8087-80]SMasamoto, Kazuto [8088-13]S2Massi, Daniela [8087-43]S10Mastanduno, Michael A. [8089-09]S2Mata Pavia, Juan [8088-37]S7Matcher, Stephen J. [8091-10]S3,

[8091-11]S3, [8091-61]SPS2Mathevon, Nicolas [8088-11]S2Mathieu, Jacques [8086-43]SPS1Matteini, Paolo [8092-30]S6Matthäus, Christian [8087-46]S11Matthews, Thomas [8087-42]S10Mayer, Markus A. [8091-19]S5Mazurenka, Mikhail [8088-09]S2,

[8088-12]S2, [8088-57]SPS1McGinty, James A. [8086-13]S3McKenna, Barbara [8087-36]S8McLean, David I. [8090-44]SPS1McLean, Irwin [8092-07]S3Meade, Aidan D. [8087-12]S4Meglinski, Igor 8090 ProgComm,

[8090-06]S1Mehnert, Jan [8088-17]S3Mehta, Kalpesh B. [8091-12]S3Meisel, Susann [8087-05]S2Meissner, Sven [8091-39]S8, [8091-

41]S8, [8091-58]SPS2Meister, Jean-Jacques [8091-04]S2Memmolo, Pasquale [8089-11]S2Meneghello, Anna [8090-41]SPS1Menna, Simona [8088-51]S10Merkus, Peter J. F. M. [8087-55]S13Mermut, Ozzy [8090-16]S3Merola, Francesco [8089-11]S2Mertz, Jerome 8086 ProgCommMetzger, Roman [8092-10]S3Meyer, Tobias [8087-30]S8Miccio, Lisa [8086-12]S2, [8089-11]

S2Michael, Jan [8087-63]SMichels, René [8092-18]S4Mienkina, Martin P. [8088-40]S8Milazzotto, Marcella P. [8092-23]S5Milej, Daniel [8088-14]S3, [8088-45]

S9, [8088-57]SPS1Minai, Limor [8092-20]S4, [8092-43]

S8Minato, Kotaro [8086-37]SPS1Mingo-Ortega, Patricia [8092-03]S1Minkoff, David L. [8088-10]S2Minzioni, Paolo [8092-22]S5Mito, Jeff [8087-17]S5Mitrani, Daniel [8088-42]S8Miura, Masahiro [8091-14]S4Miwa, Yoshihiro [8088-32]S6Mohajerani, Pouyan [8089-22]SPS1Moiseev, Alexander A. [8091-67]

SPS2Molteni, Erika [8088-01]S1Monbet, Valérie [8087-23]S6, [8087-

100]SMönch, Bettina [8087-04]S1Montcel, Bruno [8088-11]S2Montejo, Julio D. [8088-35]S6Montejo, Ludguier D. [8088-22]S4,

[8088-35]S6Moreau, Julien [8086-42]SPS1Morgan, Nicole [8086-52]SPS1Morini, Cristiano [8087-44]S10Moriyama, Lilian T. [8092-08]S3,

[8092-53]SPS2Morooka, Toshifumi [8091-34]S7Morscher, Stefan [8089-26]SPS1Motoda, Masafumi [8090-39]SPS1Mottin, Stéphane [8088-11]S2Mouras, Rabah [8086-25]S5Movania, Muhammad [8086-31]S6Mozina, Janez [8092-32]S6Mudde, Yvo [8091-03]S1Mudter, Jonas [8087-95]SMueller, Jenna [8087-17]S5Müeller, Gerhard A. [8088-35]S6Müller, Ulrich Alfons [8087-50]S12Münchberg, Ute [8087-05]S2



Munro, Ian H. [8087-25]S6, [8087-41]S9

Muratore, Massimo [8086-25]S5Murayama, Takanobu [8088-49]S9Musi, Valeria [8090-12]S2Mycek, Mary-Ann [8087-31]S8,

[8087-36]S8, [8087-502]SPLN, [8089-18]S3, [8090-14]S3

Myllyla, Risto [8091-60]SPS2, [8090-28]SPS1, [8090-31]SPS1

Myllylä, Teemu S. [8090-31]SPS1

N

Nadkarni, Seemantini K. 8090 ProgComm, 8090 S2 SessChr, [8090-07]S1

Nadort, Annemarie [8087-13]S4Nadtochenko, Viktor A. [8092-04]S1Nadyarnykh, Oleg [8087-14]S4Nagumo, Osamu [8086-40]SPS1Nakao, Keisuke [8090-39]SPS1Nakayama, Haruka [8088-13]S2Nammour, Samir [8092-58]SPS2Nanke, Ralf [8087-54]S13Näsi, Tiina [8088-05]S1Nasution, Aulia [8087-95]SNazarenko, Anton [8087-29]S7Neagu, Liviu [8091-30]S6Neel, Benjamin G. [8089-21]SPS1Negrutiu, Meda Lavinia [8091-68]

SPS2, [8091-62]SPS2Neil, Mark A. [8087-41]S9, [8087-25]

S6Nerin, Philippe [8087-62]SNetz, Uwe J. [8088-35]S6, [8088-60]

SPS1Neugebauer, Ute [8087-16]S5Neumaier, Michael [8088-53]S10Neurath, Markus [8087-95]SNgezahayo, Anaclet [8086-22]S4Nguyen, Duc V. [8091-25]S6Nguyen, Krzysztof [8086-27]S5Nichi, Marcilio [8092-23]S5Nicolodelli, Gustavo [8092-46]SPS2,

[8092-60]SPS2Niedre, Mark J. [8089-08]S2Nielsen, Poul [8091-23]S5Niessing, Michael [8088-02]S1,

[8088-03]S1Nikkinen, Juha [8090-31]SPS1Ninou, Jordi [8088-42]S8Nishidate, Izumi [8087-97]SNoordmans, Herke Jan [8087-28]S7,

[8090-40]SPS1Nozoe, Saki [8092-47]SPS2Ntziachristos, Vasilis 8089 Chr, 8089

SPS1 SessChr, 8089 S1 SessChr, [8089-02]S1, [8089-04]S1, [8089-07]S1, [8089-10]S2, [8089-15]S3, [8089-19]SPS1, [8089-20]SPS1, [8089-22]SPS1, [8089-26]SPS1, [8090-22]S4, [8090-23]S4, [8090-36]SPS1, [8090-37]SPS1, [8090-42]SPS1

Nugroho, Hermawan [8087-80]SNuster, Robert [8090-20]S4, [8090-

35]SPS1

O

Oancea, Andreas [8089-07]S1Obata, Takayuki [8088-18]S3Oh, Se Baek [8086-11]S2Okada, Eiji [8088-13]S2, [8088-18]

S3, [8088-47]S9, [8088-48]S9, [8088-50]S9, [8088-70]S

Okamoto, Takashi [8090-39]SPS1Okawa, Shinpei [8088-32]S6Olive, D. Michael 8089 ProgCommOliverio, Nestor H. [8088-42]S8Olivier, Nicolas [8086-24]S5, [8086-

34]S7Olivier, Scot S. [8091-46]S9

Olivo, Malini C. [8086-31]S6, 8087 S8 SessChr, [8087-26]S7, [8090-09]S2, [8090-11]S2

Olsovsky, Cory [8086-48]SPS1Omenetto, Fiorenzo G. [8090-46]

SJS2Onsea, Kevin [8091-33]S7Oosterhuis, J. Wolter [8090-18]S4Opitz, Jörg L. [8087-54]S13, [8087-

63]SOraevsky, Alexander A. 8089

ProgComm, 8089 S3 SessChr, [8089-01]S1

Orringer, Daniel [8089-05]S1Ortega-Quijano, Noé [8088-64]SPS1,

[8092-03]S1Ortiz d’Avila Assumpcao, Mayra E.

[8092-23]S5Ortmann, Uwe [8086-15]S3Osten, Wolfgang [8086-49]SPS1Ostendorf, Andreas [8090-24]SPS1O’Sullivan, Jack D. [8087-90]SOtto, Cees [8087-58]S13

P

Pache, Christophe [8091-04]S2, [8091-38]S8

Paeder, Vincent [8090-12]S2Paeglis, Roberts [8090-26]SPS1Pai, Vinay M. [8089-24]SPS1Palmer, Gregory M. 8087

ProgComm, [8087-60]SPaltauf, Günther 8090 ProgComm,

[8090-20]S4, [8090-35]SPS1Pampaloni, Francesco [8086-16]S3Panteleeva, Olga G. [8091-75]SPS2Panzica, Ferruccio [8088-01]S1Pardo, Olivier E. [8086-13]S3Paschalis, Eleftherios P. [8087-96]SPassler, Klaus [8090-20]S4, [8090-

35]SPS1Patalay, Rakesh [8087-41]S9Patterson, Michael S. [8092-09]S3Paturzo, Melania [8089-11]S2Paulsen, Keith D. [8087-18]S5,

[8089-09]S2Pavillon, Nicolas [8090-03]S1Pavone, Francesco S. 8086

ProgComm, 8086 S1 SessChr, [8086-18]S4, 8087 ProgComm, [8087-43]S10, [8087-44]S10

Pechprasarn, Suejit [8086-56]SPS1Pedrueza, Esteban [8092-51]SPS2Pelegati, Vitor B. [8086-20]S4Pereira, Mariette M. [8089-25]SPS1Peremans, André G. [8092-56]SPS2,

[8092-58]SPS2Perez-Gutierrez, Francisco G. [8092-

06]S1Peters, Sven [8086-04]S1Petersen, Wilma [8087-58]S13Petrescu, Emanuela L. [8091-62]

SPS2Petrik, Peter [8087-10]S3Petrov, Dmitri [8087-51]S12Petrov, Valentin P. [8092-56]SPS2Peyriéras, Nadine [8086-34]S7Peyrot, Donald [8092-35]S7, [8092-

44]S8Pezzoli, Daniele [8087-53]S13Philipp, Carsten M. 8092 ProgComm,

8092 S6 SessChrPierobon, Roberto [8090-41]SPS1Pifferi, Antonio [8088-09]S2, [8088-

12]S2, [8088-43]S9, [8088-51]S10, [8090-27]SPS1

Piletic, Ivan [8087-42]S10Pini, Roberto [8092-30]S6, [8092-55]

SPS2Piras, Daniele [8087-20]S6, [8090-21]

S4Pircher, Michael [8091-07]S3, [8091-

08]S3, [8091-09]S3, [8091-13]S4Piron, Vianney M. [8092-16]S4Piskin, Erhan [8087-08]S3

Pitris, Costas [8087-03]S1, [8089-06]S1, [8091-22]S5

Pitter, Mark C. [8086-56]SPS1Plamann, Karsten [8086-19]S4,

[8092-35]S7, [8092-44]S8Planat-Chrétien, Anne [8088-31]S6,

[8088-46]S9Podoleanu, Adrian G. 8091

ProgComm, 8091 S8 SessChr, [8091-30]S6, [8091-54]S10, [8091-62]SPS2, [8091-68]SPS2

Poggesi, Corrado [8086-18]S4Pogue, Brian W. SympChair, [8087-

18]S5, [8088-36]S7, [8089-09]S2, 8092 ProgComm

Poher, Vincent [8086-44]SPS1Pohl, Andrea [8087-63]SPolikarpov, Igor [8090-33]SPS1Pöllinger, Alexander [8088-52]S10Pollnau, Markus [8091-25]S6Poot, André A. [8087-58]S13Popov, Alexey P. [8090-28]SPS1,

[8091-60]SPS2Popp, Jürgen 8087 Chr, 8087 SPS2

SessChr, 8087 S1 SessChr, [8087-04]S1, [8087-05]S2, [8087-16]S5, [8087-30]S8, [8087-46]S11, [8087-64]S, [8087-84]S

Porcar-Guezenec, Rafael [8086-02]S1

Porro, Giampiero [8087-65]SPotapov, Aleksandr [8087-29]S7Potsaid, Benjamin M. [8091-15]S4,

[8091-19]S5Poulet, Patrick [8092-13]S4Pourreyron, Celine [8092-07]S3Pova?ay, Boris [8091-02]S1, [8091-

45]S9Po?ar, Toma? [8092-32]S6Priezzhev, Alexander V. [8090-28]

SPS1, [8091-60]SPS2Prins, Christian [8090-21]S4Przibilla, Sabine [8086-06]S2Psilodimitrakopoulos, Sotiris [8086-

21]S4, [8086-46]SPS1Ptaszynski, Lars [8092-37]S7, [8092-

38]S7, [8092-39]S7Puca, Alfredo [8092-30]S6Puglisi, Roberto [8086-12]S2, [8089-

11]S2

Q

Qi, Ji [8087-92]SQian, Kemao [8086-31]S6Qian, Zhiyu [8087-98]SQuarto, Giovanna [8088-51]S10Quesnel-Hellmann, Anne [8086-43]

SPS1Quinn, Kyle P. [8086-05]S1

R

Raczkowsky, Jörg [8092-34]S6Radrich, Karin [8089-19]SPS1Rafailov, Edik U. [8092-07]S3Raj, Saurabh [8087-51]S12Ramanujam, Nimmi [8087-17]S5,

8087 Chr, 8087 SPS2 SessChr, 8087 S10 SessChr, [8087-19]S6, [8087-60]S

Ramaz, François [8088-38]S8, [8088-39]S8

Ramgolam, Anoop [8087-21]S6Ramstein, Stéphane [8088-11]S2Randeberg, Lise L. 8087 ProgComm,

8087 S7 SessChr, [8087-40]S9Ranji, Mahsa [8087-07]S3Rao, Devulapalli V. G. L. N. [8090-05]

S1Rao, Satish [8087-51]S12Ratto, Fulvio [8092-01]S1Ravelo, Rasata [8092-13]S4

Ray, Aniruddha [8089-05]S1Rayavarapu, Rajagopal [8087-58]S13Razansky, Daniel [8089-02]S1, [8089-

04]S1, [8089-07]S1, [8089-20]SPS1, [8089-26]SPS1, [8090-22]S4, [8090-23]S4, [8090-36]SPS1, [8090-37]SPS1, [8090-42]SPS1

Read, Mike D. [8091-73]SPS2Reble, Carina [8087-83]S, [8087-94]SRegar, Evelyn [8091-32]S7Rego, Raquel F. [8092-46]SPS2Rego Filho, Francisco [8092-54]

SPS2, [8092-59]SPS2Rehn, Simon [8088-46]S9Reichart, Rupert [8087-30]S8Rička, Jaroslav [8088-19]S4Ricco, Raffaele [8090-41]SPS1Rice, Tyler B. [8087-18]S5Richter, Verena [8089-12]S2Rink, Alexandra [8090-16]S3Rinneberg, Herbert H. [8088-52]S10Ripken, Tammo [8092-42]S8, [8092-

45]S8Ritsch-Marte, Monika A. 8091 SJS1

SessChrRobinson, Dominic J. [8087-15]S5,

8092 ProgCommRodier, Jean François [8092-13]S4Rogule, Laura [8087-82]SRoider, Johann [8092-37]S7Rolle, Udo [8092-10]S3Rollins, Andrew M. 8091 ProgComm,

[8091-34]S7Romanov, Oleg G. [8088-64]SPS1Romeike, Bernd F. M. [8087-30]S8,

[8087-46]S11Rongeat, Nelly [8087-62]SRösch, Petra [8087-04]S1, [8087-05]

S2Rosenhahn, Axel [8091-74]SPS2Rosenthal, Amir [8089-20]SPS1,

[8090-23]S4Rossi, Francesca [8092-30]S6,

[8092-55]SPS2Rossi, Giacomo [8092-30]S6Rossi Sebastiano, Davide [8088-01]

S1Rothe, Thomas [8086-38]SPS1,

[8086-39]SPS1Rotin, Daniil [8087-29]S7Rotomskis, Ricardas 8092

ProgCommRubenis, Oskars [8087-85]S, [8090-

29]SPS1Rubins, Uldis [8087-85]SRudnitzki, Florian [8092-01]S1Rudzitis, Andris [8090-26]SPS1Ruers, Theo J. M. [8091-42]S8Runkel, Frank [8086-45]SPS1, [8086-

57]SPS1Runnels, Judith M. [8089-08]S2Rutt, Harvey N. [8087-90]S

S

Sá, Gonçalo [8089-25]SPS1Sabates, Felix N. [8091-71]SPS2Sabates, Nelson R. [8091-71]SPS2Sablong, Raphael [8087-21]S6Sacconi, Leonardo [8086-18]S4Saetchnikov, Vladimir A. [8090-24]

SPS1Sagher, Oren [8089-05]S1Saiki, Masayuki [8092-05]S1Saino, Enrico [8092-22]S5Saint-Jalmes, Hervé [8087-21]S6Saiz, Ricardo [8088-42]S8Sakota, Daisuke [8092-26]S5Salas-García, Irene [8088-64]SPS1,

[8092-03]S1Saldua, Meagan A. [8086-48]SPS1Salgado, Jesús [8092-51]SPS2Salvador Bagnato, Vanderlei [8090-

33]SPS1, [8092-11]S3, [8092-46]SPS2, [8092-53]SPS2, [8092-54]SPS2, [8092-59]SPS2, [8092-60]SPS2, [8092-61]SPS2



Samim, Masood [8086-60]SSamineni, Prathyush [8086-23]S5Sampson, David D. 8091 ProgComm,

[8091-21]S5, [8091-27]S6Sanchez, Jean-Charles [8087-10]S3Sánchez, Orlando [8092-51]SPS2Santi, Sara [8090-12]S2Santos i Lleo, Andres [8086-34]S7Sarantopoulos, Athanasios [8089-15]

S3, [8089-26]SPS1Sardini, Alessandro [8086-13]S3Sato, Manabu [8087-97]SSato, Shunichi [8087-97]SSattmann, Harald [8091-07]S3Sauer, Benjamin [8086-47]SPS1Sauvage, Vincent [8087-92]SSaveljeva, Tatjana [8087-29]S7Savitsky, Alexander P. [8089-23]

SPS1Savoldelli, Michèle [8092-44]S8Savy, Thierry [8086-34]S7Sawosz, Piotr [8088-12]S2, [8088-15]

S3, [8088-45]S9, [8088-57]SPS1Saxena, Vishal [8088-69]SScerrati, Alba [8092-30]S6Schaberle, Fábio [8089-25]SPS1Schädel, Daniela [8087-94]SSchäfer, Jan [8090-17]S3Schak, Matthias [8087-67]SSchanne-Klein, Marie-Claire [8086-

19]S4Scharfetter, Hermann [8088-62]

SPS1, [8088-63]SPS1Schausberger, Stefan E. [8091-06]S2Scheiman, James [8087-36]S8Schelkanova, Irina [8088-58]SPS1Schiaffi, Elena [8088-01]S1Schiffer, Thorsten [8087-67]SSchirner, Michael [8087-32]S8Schlag, Peter M. [8088-52]S10Schlott, Kerstin [8092-37]S7, [8092-

39]S7Schlücker, Sebastian [8087-64]SSchlupp, Peggy [8086-45]SPS1,

[8086-57]SPS1Schmid, Simone [8088-06]S2Schmidt, Henning [8088-06]S2Schmidt, Michael [8087-95]SSchmidts, Thomas [8086-45]SPS1,

[8086-57]SPS1Schmitt, Robert [8091-59]SPS2Schmitz, Christoph H. [8088-17]S3Schmitz, Georg [8088-40]S8Schmitz, Michael [8086-38]SPS1,

[8086-39]SPS1Schmoll, Tilman [8091-07]S3, [8091-

16]S4, [8091-17]S4Schnabel, Christian [8091-39]S8,

[8091-58]SPS2Schneckenburger, Herbert [8089-12]

S2Schneider, Achim [8087-94]SSchneider, Dankiel [8088-53]S10Schober, Lena [8091-59]SPS2Schoeffel, Markus D. [8089-17]S3Schreiber, Jürgen [8087-54]S13,

[8087-63]SSchultz, Michael [8092-24]S5Schumacher, Wilm [8087-04]S1,

[8087-05]S2Schunemann, Peter G. [8092-56]

SPS2Schwabe, Kerstin [8086-22]S4Schweiger, Gustav [8090-24]SPS1Schweitzer, Dietrich [8086-04]S1,

[8087-50]S12Schweizer, Julia [8087-49]S12Scott, Benjamin [8090-10]S2Seah, Hock-Soon [8086-31]S6Selim, Maria [8087-42]S10Sendra, Gonzalo H. [8091-74]SPS2Senesi, Folco [8087-47]S12Sepehr, Reyhaneh [8087-07]S3Serafetinides, Alexander A. [8092-36]

S7, [8092-21]S5Sergiadis, George [8089-04]S1Sergon, Mildred [8087-54]S13Serpa, Carlos [8089-25]SPS1Seyed Reihani, Seyed Nader [8092-

02]S1

Shaffer, Etienne [8086-08]S2Shah, Amy [8087-60]SShakhova, Natalia M. 8091

ProgComm, [8091-75]SPS2Shapovalov, Valentine V. [8087-75]SShcheslavskiy, Vladislav I. [8090-15]

S3Shelton, Ryan L. [8086-48]SPS1,

[8090-47]SJS2Shepherd, Neil [8087-22]S6Sheppard, Colin J. R. [8086-36]SPS1Shevelev, Ivan [8087-29]S7Shi, Jiyun [8089-21]SPS1Shilyagin, Pavel A. [8091-67]SPS2Shirmanova, Marina V. [8092-04]S1Shkandina, Tanya [8087-69]SSigal, Brigitte [8091-37]S8Sikkel, Markus B. [8086-30]S6Silien, Christophe [8092-56]SPS2Simeone, Diane [8087-36]S8Simon, Herve [8092-13]S4Simpson, Mary Jane [8087-42]S10Sinescu, Cosmin [8091-62]SPS2,

[8091-68]SPS2Singh, Amardeep S. G. [8091-16]S4,

[8091-17]S4Sinnaeve, Peter [8091-33]S7Siozade-Lamoine, Laure [8091-49]S9Siqueira, Adriano [8092-23]S5Sirotkina, Marina A. [8092-04]S1Skala, Melissa C. 8087 ProgCommSo, Peter T. C. 8086 Chr, 8086 S2

SessChr, 8086 SPS1 SessChr, 8086 S5 SessChr, [8086-58]SPS1

Soares, Carlos A. [8087-35]S8Soh, Kiat Seng [8090-09]S2Sokolov, Konstantin V. 8089 S2

SessChr, [8089-13]S3Sokolovski, Sergei G. [8092-07]S3Solinas, Xavier [8086-24]S5, [8086-

34]S7Soloviev, Vadim Y. [8086-13]S3,

[8088-26]S5Somekh, Michael G. [8086-56]SPS1Soo, Khee-Chee [8086-31]S6Sorvoja, Hannu [8090-31]SPS1Soudamini Amma, Dinish U. K.

[8090-09]S2, [8090-11]S2South, Andrew P. [8092-07]S3Spaniol, Stefan B. 8092 ProgCommSpigulis, Janis [8087-82]S, [8087-85]

SSpinelli, Lorenzo [8088-01]S1, [8088-

08]S2, [8088-09]S2, [8088-12]S2, [8088-14]S3, [8088-51]S10, [8088-57]SPS1

Sprik, Rudolf [8091-03]S1Spunde, Andris [8090-26]SPS1Spyratou, Ellas [8092-21]S5, [8092-

36]S7Sreedharan, Rajesh [8086-24]S5Srinivasan, Subha [8088-36]S7, 8088

ProgComm, 8088 S5 SessChr, 8088 S6 SessChr, [8088-66]S, [8088-67]S

Sroka, Ronald 8092 SPS2 SessChr, 8092 S5 SessChr, 8092 S6 SessChr, 8092 Chr, [8092-28]S6

St. Lawrence, Keith [8088-55]SPS1Stahl, Pierre [8088-44]S9Stamp, Gordon W. [8087-25]S6,

[8087-41]S9Staniszewski, Kevin [8087-07]S3Steenbergen, Wiendelt [8087-20]S6,

[8089-03]S1, [8090-21]S4Steenkeste, Karine [8087-52]S13Steibel, Jerome [8092-13]S4Steimers, Andre [8088-07]S2Steinbrink, Jens M. [8088-14]S3,

[8088-17]S3Steiner, Gerald [8087-49]S12Steingräber, Robert [8088-06]S2Steinkellner, Oliver [8088-06]S2,

[8088-12]S2, [8088-14]S3, [8088-57]SPS1

Stelzer, Ernst H. K. 8086 ProgComm, [8086-33]S7

Stepp, Herbert 8092 ProgComm, 8092 S3 SessChr, [8092-10]S3

Sterenborg, Henricus J. C. M. 8087 S6 SessChr, 8090 S4 SessChr, 8090 SPS1 SessChr, 8090 Chr, [8087-14]S4, [8087-15]S5

Sterry, Wolfram [8092-14]S4Stewart, Jocelyn [8089-21]SPS1Stewart, Neil Z. [8092-07]S3Stöckel, Stephan [8087-05]S2Stoica, Eniko Tunde [8091-68]SPS2Stolyarenko, Georgy [8091-44]S8Stone, Nicholas [8087-22]S6, [8091-

02]S1, [8091-73]SPS2Stoyanov, Danail [8090-01]S1Straus, Andreas [8088-40]S8Strauss, Wolfgang S. L. [8089-12]S2Strömberg, Tomas [8087-48]S12,

[8087-56]S13Stroumpinis, Theodoros [8092-36]S7Stuckey, Daniel W. [8086-13]S3Styles, Iain B. [8087-81]S, [8088-56]

SPS1Su, Ping-Jung [8087-33]S8Subhash, Hrebesh M. [8091-05]S2Subramaniam, Vinod 8086

ProgCommSüdmeyer, Thomas [8092-19]S4Suenaga, Emi [8086-40]SPS1Sugiura, Tadao [8086-37]SPS1Summer, Stefan [8092-40]S8Sun, Chia-Wei [8087-27]S7, [8091-

43]S8Supatto, Willy [8086-35]S7Suzen, Mehmet [8088-25]S5Svanberg, Katarina Review, Review,

[8087-38]S9, Review, Review, Review, Review, Review

Swarat, Dominic [8087-77]SSylwestrzak, Marcin [8091-28]S6Szabó, Zoltán [8087-48]S12Szkulmowski, Maciej [8091-28]S6,

[8091-48]S9Szlag, Daniel [8091-28]S6

T

Tabatabaei, Nima [8090-48]STachtsidis, Ilias [8088-02]S1, [8088-

03]S1Tahara, Satoko [8091-34]S7Tai, Qiangqiang [8090-38]SPS1,

[8090-43]SPS1Takagaki, Masatoshi [8088-07]S2Takahashi, Yosuke [8088-18]S3Takano, Shoji [8088-47]S9Takatani, Setsuo [8092-26]S5Takuwa, Hiroyuki [8088-13]S2Talary, Mark S. [8087-74]STalbot, Clifford B. [8087-41]S9Tam, Natalie [8090-10]S2Tandjung, Stephanus S. [8086-31]S6Targowski, Piotr [8091-28]S6Taroni, Paola [8087-53]S13, 8088

Chr, 8088 S7 SessChr, 8088 SPS1 SessChr, 8088 S9 SessChr, [8088-43]S9, [8088-51]S10, [8090-27]SPS1

Taruttis, Adrian [8090-22]S4Taubert, Dieter R. [8088-57]SPS1Taubes, Alice [8087-51]S12Taylor, Harriet B. [8086-13]S3Tcherniavskaia, Elina A. [8090-24]

SPS1Tchvialeva, Lioudmila [8090-44]SPS1Tearney, Gary [8091-32]S7Tellier, Franklin [8092-13]S4Ten Hagen, Timo [8089-03]S1Terpelov, Dmitry A. [8091-44]S8Terstappen, Leon W. M. M. [8087-58]

S13Tervonen, Osmo [8090-31]SPS1Terwagne, Guy [8092-58]SPS2Tesi, Chiara [8086-18]S4Teudt, Ingo U. [8092-24]S5Theelen, Thomas [8091-57]SPS2Theisen-Kunde, Dirk [8092-29]S6Themelis, George [8089-15]S3Thillainayagam, Andrew [8087-25]S6

Thomas, Giju [8087-14]S4Thompson, Alex J. [8087-25]S6Thong, Patricia S. P. [8086-31]S6Tian, Qinghai [8089-16]S3Tielemans, Marc [8092-58]SPS2Till, Holger [8092-10]S3Tinne, Nadine [8092-45]S8Tobita, Mari [8088-10]S2Toelsner, Jan [8088-60]SPS1Tolstik, Alexei L. [8088-64]SPS1Toma, Marieta [8087-54]S13Tomin, Andriy [8087-69]STominaga, Junji [8086-40]SPS1Topala, Florin Ionel [8091-62]SPS2,

[8091-68]SPS2Toronov, Vladislav Y. [8088-55]SPS1,

[8088-58]SPS1Torricelli, Alessandro [8088-01]S1,

[8088-08]S2, [8088-12]S2, [8088-14]S3, [8088-51]S10, [8088-57]SPS1

Torzicky, Teresa [8091-08]S3, [8091-09]S3, [8091-13]S4

Tosi, Alberto [8088-09]S2, [8090-27]SPS1

Tournier, Jean-Nicolas [8086-43]SPS1

Toussaint, Jimmy D. [8090-07]S1Tribioli, Jeison T. [8092-60]SPS2Trifanov, Irina [8091-30]S6Troiano, Michela [8087-44]S10Tromberg, Bruce J. [8087-18]S5,

[8092-14]S4Trono, Cosimo [8087-47]S12, [8087-

65]STruong, Thai V. [8086-35]S7Trzcinski, Robert [8092-31]S6Tsaousi, Eleni [8092-36]S7Tunnel, James 8087 ProgCommTurchin, Ilya V. [8089-23]SPS1Turck, Natacha [8087-10]S3Turker, Burak [8087-08]S3Tyndall, David [8086-27]S5

U

Uchugonova, Aisada A. [8086-03]S1Ughi, Giovanni J. [8091-33]S7Uglov, Alexander S. [8088-28]S5Unterhuber, Angelika [8091-17]S4Urankar, Alexandra [8087-62]SUtzinger, Urs [8086-50]SPS1, 8087

ProgCommUzunbajakava, Natallia [8090-38]

SPS1, [8090-43]SPS1

V

Vabres, Pierre [8087-80]Svan Beurden, Marc [8091-42]S8van Beusekom, Heleen M. M. [8090-

18]S4van Boven, Hester [8091-42]S8Van Cleuvenbergen, Stijn J. [8086-

46]SPS1van Dam, Gooitzen M. 8089

ProgCommvan den Engh, Frank [8087-20]S6van der Steen, Antonius F. W. [8090-

18]S4, [8091-32]S7Van der Steen, Sander [8090-40]

SPS1van der Steen, Ton [8090-19]S4van Es, Peter [8087-58]S13van Hespen, Johan [8087-20]S6,

[8090-21]S4van Leeuwen, Ton G. [8087-13]

S4, [8087-20]S6, [8087-58]S13, [8089-03]S1, [8090-21]S4, 8091 ProgComm, [8091-03]S1, [8091-25]S6, [8091-40]S8, [8091-42]S8, [8091-51]S10, [8091-52]S10

van Marle, Jan [8091-52]S10



Van Neck, Han [8089-03]S1van Soest, Gijs 8090 ProgComm,

[8090-18]S4, [8090-19]S4, 8091 ProgComm, [8091-32]S7

van Stralen, Marijn [8090-40]SPS1van Tinteren, Harm [8091-42]S8van Veen, Robert L. [8087-15]S5Van Veldhoven, Spiridon [8090-21]S4van Voskuilen, Johan [8087-14]S4Vanderleyden, Jozef [8086-46]SPS1Vanholsbeeck, Frédérique [8091-23]

S5, [8091-76]SVanzetta, Ivo [8088-44]S9Varghese, Babu [8090-38]SPS1,

[8090-43]SPS1Vasile, Liliana [8091-68]SPS2Vasyliv, Oresta M. [8087-76]SVeilleux, Israel [8086-34]S7Veraksa, Alexey [8090-05]S1Verdaasdonk, Rudolf M. [8087-28]

S7, 8092 ProgComm, [8092-56]SPS2

Vergnole, Sébastien [8091-29]S6Verhagen, Rieko [8090-38]SPS1,

[8090-43]SPS1Verma, Ankur [8086-61]SVerpillat, Frederic [8090-49]S, [8090-

04]S1Vial, Jean-Claude A. [8086-43]SPS1Vickers, Dwayne [8089-08]S2Vieira, Edson [8092-59]SPS2Viellerobe, Bertrand [8087-25]S6Vignal, Clémentine [8088-11]S2Villa, Anna M. [8088-51]S10Villeneuve, Alain [8091-29]S6Villiger, Martin [8091-04]S2Villiger, Martin L. [8091-38]S8Villoslada, Pablo [8087-51]S12Vincent, Ryan D. [8091-71]SPS2Virtanen, Jaakko [8088-05]S1Visai, Livia [8092-22]S5Visani, Elisa [8088-01]S1Vishwanath, Karthik [8087-60]SVitkin, Alex 8090 Chr, 8090 SPS1

SessChr, 8090 S1 SessChrVizhanyo, Attila [8092-40]S8Vlasov, Vitaly V. [8088-28]S5Vogel, Alfred 8092 ProgComm, 8092

S8 SessChrVoigt, Jan [8087-32]S8Voit, Florian [8090-17]S3Voitcu, Gabriel [8092-25]S5Vollet Filho, José D. [8087-02]S1,

[8092-08]S3Vollmer, Angelika [8086-06]S2Volonterio, Alessandro [8087-53]S13von Bally, Gert [8086-06]S2Voormolen, Eduard [8090-40]SPS1

W

Wabnitz, Heidrun 8088 ProgComm, 8088 S1 SessChr, [8088-02]S1, [8088-03]S1, [8088-06]S2, [8088-09]S2, [8088-12]S2, [8088-14]S3, [8088-57]SPS1

Wagner, Thorsten [8087-77]SWaharte, François [8087-52]S13Wahl, Michael [8086-15]S3Walker, Richard [8086-27]S5Walkin, Louise [8091-66]SPS2Walles, Heike [8091-59]SPS2Walter, Angela [8087-04]S1Walther, Julia [8091-18]S4, [8091-31]

S7, [8091-41]S8, [8091-56]SPS2Wang, Bingqing [8087-60]SWang, Chun-Yang [8087-27]S7,

[8091-43]S8Wang, Irene [8086-27]S5Wang, Jingyu [8091-54]S10Wang, Jiongjiong [8088-10]S2Wang, Sheng-Shun [8092-50]SPS2Wang, Xueding [8089-05]S1Wang, Xuena [8087-98]SWang, Zhao [8091-34]S7Warber, Michael [8086-49]SPS1Warnecke, Jan [8087-67]SWarren, Sean [8087-41]S9Warren, Warren S. [8086-23]S5,

[8087-42]S10Watson, Alastair J. M. 8087

ProgCommWatts, Sam [8087-10]S3Wawro, Debra D. [8090-25]SPS1Weber, Petra [8089-12]S2Weber, Philipp [8087-63]SWeigl, Wojciech [8088-14]S3, [8088-

15]S3Weingarten, Kurt J. [8092-19]S4Weissbach, Carmen [8087-32]S8Weisse, Sebastian [8091-74]SPS2Welker, Pia [8087-32]S8Wells, Wendy A. [8087-18]S5Welp, Hubert [8091-65]SPS2Welzel, Julia 8091 ProgCommWen, Han H. [8089-24]SPS1, [8086-

52]SPS1Wendt, Thomas [8087-67]SWenzel, Gentiana I. [8092-24]S5Werner, John S. [8091-46]S9Wessels, Ronni [8091-42]S8Wiemann, Martin [8087-77]SWieser, Wolfgang [8091-08]S3,

[8091-24]S6, [8091-55]SPS2Wiethoff, Helge [8091-65]SPS2Wilding, Dean [8086-30]S6Wilke, Lee [8087-19]S6Willett, Rebecca M. [8087-17]S5Wilson, Brian C. [8089-14]S3, [8090-

10]S2Wilson, Robert H. [8087-31]S8,

[8087-36]S8, [8089-18]S3, [8090-14]S3

Wilson, Tony 8086 ProgCommWirth, Manfred [8087-54]S13

Wise-Milestone, Lisa [8089-14]S3Witte, Stefan M. [8091-500]SJS1Wittig, Rainer [8089-12]S2Woerdeman, Peter [8090-40]SPS1Wojtkiewicz, Stanislaw [8088-15]S3Wojtkowski, Maciej 8091 ProgComm,

8091 S10 SessChr, [8091-48]S9Wolf, Martin 8088 ProgComm, 8088

S2 SessChr, 8088 S3 SessChr, [8088-37]S7

Wolken, Heike [8092-29]S6Wood, James J. [8087-22]S6Wörhoff, Kerstin [8091-25]S6Wörn, Heinz [8092-34]S6Wu, Binlin [8088-33]S6Wynne, James J. [8092-31]S6

X

Xaio, Xianghui [8086-52]SPS1Xia, Wenfeng [8087-20]S6, [8090-21]

S4Xu, Chris 8090 ProgCommXu, Min [8088-33]S6Xu, Ming [8087-37]S8

Y

Yalavarthy, Phaneendra K. [8088-29]S5

Yamada, Yukio 8088 ProgComm, 8088 S1 SessChr, 8088 S2 SessChr, [8088-32]S6

Yamamoto, Hiroaki [8088-32]S6Yamamoto, Kazuyo [8092-05]S1Yan, Ping [8086-18]S4Yang, Guang-Zhong [8090-01]S1Yang, Qiubao [8087-70]SYang, Rong [8088-67]SYang, Shan-Yi [8091-69]SPS2Yang, Tianyue [8087-70]S, [8087-71]

S, [8087-72]S, [8087-78]S, [8087-79]S

Yanina, Irina Y. [8092-33]S6Yaremyk, Roman Y. [8086-55]SPS1Yasuno, Yoshiaki 8091 ProgComm,

8091 S4 SessChr, [8091-14]S4, [8091-47]S9

Yasuo, Kenzo [8092-05]S1Yelin, Dvir [8086-32]S6, [8092-20]S4,

[8092-43]S8Yelleswarapu, Chandra S. [8090-05]

S1Yiu, Kwok-wing [8086-62]SYodh, Arjun G. [8088-10]S2Yoshida, Yusuke [8088-50]S9Yoshikawa, Kazushi [8092-05]S1Yu, Bing 8087 ProgComm, 8087 S4

SessChr, [8087-19]S6, [8087-60]SYu, Hsing Cheng [8086-53]SPS1Yuen, Clement [8087-11]S3Yunusova, Ekaterina [8091-75]SPS2Yurtsever, Gunay [8091-26]S6

Z

Zaccanti, Giovanni [8088-12]S2Zagaynova, Elena V. [8092-04]S1Zaharans, Janis [8090-29]SPS1Zakharov, Pavel [8087-74]SZamfirescu, Marian [8092-41]S8Zane, Lukstina [8090-30]SPS1Zanirato Lizarelli, Rosane de Fátima

[8092-60]SPS2Zappa, Franco [8090-27]SPS1Zawadzki, Robert J. [8091-46]S9Zawilski, Kevin T. [8092-56]SPS2Zegelin, Andrea [8092-38]S7Zelenkov, Petr [8087-29]S7Zervantonakis, Ioannis [8086-11]S2Zettergren, Eric W. [8089-08]S2Zhang, Jing [8086-56]SPS1Zhang, Yaokun [8092-34]S6Zheng, Gang 8089 ProgComm,

[8089-14]S3, [8089-21]SPS1, [8090-10]S2, 8092 ProgComm

Zheng, Ronger [8087-37]S8Zheng, Wanquan [8087-09]S3Zhu, Qun [8088-36]S7Zidouk, Amaria [8087-81]SZimmerman, Shelby [8090-25]SPS1Zingoni, Tiziano [8092-55]SPS2Zirak, Peyman [8088-04]S1Zolek, Norbert S. [8088-14]S3, [8088-

15]S3, [8088-45]S9Zolotovskaya, Svetlana A. [8092-07]

S3Zotter, Stefan [8091-09]S3, [8091-13]

S4, [8091-08]S3Zoulinakis, George [8092-36]S7Zucchelli, Lucia [8088-01]S1, [8088-

08]S2, [8088-12]S2, [8088-14]S3Zupanski-Nielsen, Donna [8092-31]

S6Zvyagin, Andrei V. [8090-28]SPS1



SEARCHABLE CD-ROMS WITH MULTIPLE CONFERENCES.

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broad topical area, choose the searchable CD-ROMs. Available within 8 weeks of the meeting; PC, Macintosh, and Unix

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PROCEEDINGS ANDSEARCHABLE CD-ROMsOF

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Vol# Title/Editor Prepublication Price

8086 Advanced Microscopy Techniques II . . . . . . . . . . . . . . . . . . . .€60 ($80) (P. T. So/E. Beaurepaire)

8087 Clinical and Biomedical Spectroscopy and Imaging II . . . . .€93 ($125)(N. Ramanujam/J. Popp)

8088 Diffuse Optical Imaging III . . . . . . . . . . . . . . . . . . . . . . . . . . . . .€65 ($90)(A. H. Hielscher/P. Taroni)

8089 Molecular Imaging III . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .€40 ($53)(C. P. Lin/V. Ntziachristos)

8090 Novel Biophotonic Techniques and Applications . . . . . . . . . .€52 ($70) (H. J. Sterenborg/I. Vitkin)

8091 Optical Coherence Tomography and Coherence Techniques V . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .€75 ($100) (R. A. Leitgeb/B. E. Bouma)

8092 Medical Laser Applications and Laser-Tissue Interactions V . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .€65 ($90) (R. Sroka/L. D. Lilge)

SPIE/OSA European Conferences on Biomedical Optics 2011(Includes Vols. 8086-8092)

Order No. CDS445 • Est. pub. July 2011Meeting attendee: €125 ($170)Nonattendee member price: €320 ($430)Nonattendee nonmember price: €420 ($560)

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