ENTEROBACTERIACEAE(Klebsiella, Salmonella, Shigella)

Prepared by:Miss Norzawani Jaffar

Bsc (Hons) Biomedical Sciences, UKM

Learning Outcome• At the end of learning process student must be able

to;

– Describe characteristic of Enterobactericeae– Describe the specific characteristic for Klebsiella,

Salmonella and Shigella– Understand the role in disease for Klebsiella,

Salmonella and Shigella.– Explain the significant laboratory diagnosis for

Klebsiella, Salmonella and Shigella identification.

Characteristic

Klebsiella

Salmonella

Shigella

Confirmatory test• Microscopy of stool samples show manu white blood cells

but few if any organism• Culture and biochemistry show no motility, no gas from

glucose and no lactose fermentation.• Serology distinguishes serotypes of all except S. sonnei.

Laboratory Diagnosis

Laboratory Diagnosis

In most laboratories a metronidazole antibiotic disc is placed at the juction of the primary and second streak of the anaerobic blood plates (78a).The plate is incubated in an anaerobic cabinet and examined at disc identifies anaerobes, and a report is issued stating ‘anaerobes present; metronidazole sensitive’ (78b).

END………. Q & A??