Nat ional I nst itute For Cellular B iotechnology

Increasing Biopharma production

efficiency using DECOY-7

N a t i o n a l I n s t i t u t e f o r C e l l u l a r B i o t e c h n o l o g y ,

D u b l i n C i t y U n i v e r s i t y , D u b l i n , I r e l a n d

e m m a . o n e i l l @ i n v e n t . d c u . i e

Biopharma Challenges

• High Cost of Biologics Production

• Greater Demands on Cell Line Development

• Higher Titres

• Higher Cell Densities

• Greater pressure on pipelines (turnaround, etc.)

Greater Efficiency

Improving processes

using miRNAs

microRNAs:

• Post-transcriptional regulators

• Many targets

• Known to influence many processes

in cell

• Not translated

miRNA-7 as a Process Target• microRNA-7 (miR-7)

• Active following temperature shift (37°C-31°C)

• miR-7-depletion tool: DECOY-7

• Functional studies demonstrate miR-7 depletion has a beneficial effect on

CHO bioreactor performance:

• increased cell density

• increased viability

• US8,476,244) EP 2046970 B1

Gammell et al. 2007. Initial identification of low temperature and culture stage induction of miRNA expression in suspension CHO-K1 cells. Journal of Biotechnology, 130, 3, pp213-218Barron et al. 2011. Engineering CHO cell growth and recombinant protein productivity by overexpression of miR-7. J.Biotechnology151(2):204-11Sanchez et al. 2013. MiR-7 triggers cell cycle arrest at the G1/S transition by targeting multiple genes including Skp2 and Psme3. PLOS One. 8(6):e65671

increased product yield

DECOY-7: Cell Density

Ctrl K1

DECOY-7 K1

DECOY-7: Viability

Ctrl K1

DECOY-7 K1

DECOY-7: Cell Productivity

Ctrl K1

DECOY-7 K1

What DECOY-7 means for you

• These features together have the potential to:

• increase per-run profitability,

• decrease time required to deliver requisite titres,

• facilitate downstream purification

How we can work with you

Collaborate to find a comprehensive solution to your upstream cell line

development challenges;

- Transient transfection of your existing processes (as a media additive)

- Licence/technology transfer to implement DECOY-7 under your in-house

control

- Collaborative research on your specific cell lines at the NICB

Scientif ic Team

Biopharma & CHO Cell Engineering Group

Multidisciplinary research centre, with over 25 years experience in integrated

research in fundamental and applied cellular biotechnology, molecular cell

biology and biological chemistry

The mission of the NICB is to provide targeted and applied solutions to

challenges facing the Biopharma industry, utilising our unique multi-disciplinary

team, our extensive clinical & industry network and our collaborative problem-

solving expertise to deliver value for stakeholders, collaborators and patients.

Nat ional I nst itute For Cellular B iotechnology

Contact details

N i a l l B a r r o n , D i r e c t o r , N I C B

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P a d r a i g D o o l a n , S e n i o r R e s e a r c h F e l l o w , N I C B

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E m m a O ’ N e i l l , D i r e c t o r o f B u s i n e s s D e v e l o p m e n t , L i f e S c i e n c e s D C U

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