ecophysiological characterization of coccoloba uvifera l.: the case of a salt and drought stress...

Ecophysiological characterization ofEcophysiological characterization of Coccoloba uvifera Coccoloba uvifera L.: L.: the case of a salt and drought stress tolerant plant the case of a salt and drought stress tolerant plant species for sand dune reclamation in Cubaspecies for sand dune reclamation in Cuba

Prof.Dr. Raúl Carlos López SánchezProf.Dr. Raúl Carlos López SánchezLocal promoterLocal promoter

Overall Academic Objective (OAO): strengthen research and training on abiotic, especially salt and drought, stress of economically interesting species at Granma University (GU). Overall Developmental Objective (ODO): improve capacity building for research in salt and drought stress at GU.Specific Academic Objective (SAO): strengthen research and training on ecophysiological, nutritional and phyto-chemical characterization and fruit production under salt and drought stress of C. uvifera in saline coastal soils of Cuba. Specific Developmental Objective (SDO): develop the capacities for ecophysiologically trained researchers to reduce climate change vulnerability of the coastal areas of Cuba.


Intermediate Results (IR)

IR1. The ecophysiological mechanisms for salt and drought stress tolerance of C. uvifera are known.

IR2. Nutritional and phytochemical compounds of C. uvifera are characterized and known.

IR3. The visibility of the Abiotic Stress Group of the Biotechnology Study Centre, as an excellence center for ecophysiological salt and drought stress research in Cuba has increased


IR1. The ecophysiological mechanisms for salt and drought stress tolerance of C. uvifera are known.1.1. Preparation of experiments1.1.2 Literature review about drought and salt stress in relation to the selected and similar species


IR1. The ecophysiological mechanisms for salt and drought stress tolerance of C. uvifera are known.1.1. Preparation of experiments1.1.3 Definition of experimental design and practical requirements

Playa Las Coloradas, Granma provinceLatitude: 19° 56' 00" NLongitude: 77° 41' 00" W

Playa Guardalavaca, Holguin provinceLatitude: 21°07′30″N Longitude: 75°49′44″O

Cayo Coco, Ciego de Avila provinceLatitude: 22º 32' 10.06" NLongitude: 78º 21' 19.44" W

Playa Ancon, Sancti Spiritus provinceLatitude: 21° 44' 00" NLongitude: 80° 01' 00" W

Playa Rancho Luna, Cienfuegos provinceLatitude: 22°02′20″NLongitude: 80°25′38″W

Playa Varadero, Matanzas provinceLatitude: 23°10'28"NLongitude: 81°12'43"W

NorthNorth SouthSouth

EastEast

MiddleMiddle

WestWest

IR1. The ecophysiological mechanisms for salt and drought stress tolerance of C. uvifera are known.1.1. Preparation of experiments1.1.3 Definition of experimental design and practical requirements


IR1. The ecophysiological mechanisms for salt and drought stress tolerance of C. uvifera are known.1.1. Preparation of experiments1.1.5 Training of the personnel responsible for the manipulation of the equipment and the measurements

IR1. The ecophysiological mechanisms for salt and drought stress tolerance of C. uvifera are known.1.2. Experiments in controlled conditions1.2.1 Evaluation of the response to salt and drought stress of C. uvifera during germination



Salt stress

Control 5 dS.m-1

25 dS.m-115 dS.m-1



Drought stress

100 % field capacity

50 % field capacity

0% field capacity

IR1. The ecophysiological mechanisms for salt and drought stress tolerance of C. uvifera are known.1.2. Experiments in controlled conditions1.2.2 Evaluation of ecophysiological responses (gas exchange, photosynthesis, chlorophyll fluorescence, plant-water-relations, leaf area index) of C. uvifera to salt and drought stress at seedling and sapling stage.

Control

15 dS.m-1

Salt stress


Control 15 dS.m-1

Control 15 dS.m-1

Salt stress


Salt stress

1.3. Experiments in field conditions.1.3.2 Fruit collection of C. uvifera accessions in selected ecosystems.

Playa Las Coloradas, Granma provinceLatitude: 19° 56' 00" NLongitude: 77° 41' 00" W

Playa Guardalavaca, Holguin provinceLatitude: 21°07′30″N Longitude: 75°49′44″O

NorthNorth SouthSouth

EastEast

IR2. Nutritional and phytochemical compounds of C. uvifera are characterized and known.2.1 Intensive literature review on C. uvifera about the nutritional and medicinal compounds

IR2. Nutritional and phytochemical compounds of C. uvifera are characterized and known.2.2 Evaluation of nutritional quality of C. uvifera2.3 Phytochemical screening and antibacterial activity evaluation of C. uvifera

Dcm Et Ac Dcm Et Ac Dcm Et Ac Dcm Et Ac Dcm Et Ac Dcm Et Ac Dcm Et Ac Dcm Et Ac Dcm Et AcAlcaloides - + - - +++ - - +++ - - +++ - - +++ - - +++ - - +++ - - +++ - - +++ -Coumarinas ++ ++ ++ ++ ++ ++ + ++ ++ ++ ++ ++ + ++ ++ ++ ++ ++

Ácidos grasos - - + - - + - - +Resinas - - - - - - - - -

Triterpenos y/o esteroides - - - + - - + - -Saponinas - - - - - - - - - - - - - + - + - +

Aminoácidos libres - + + + - - + + - - - +Glicósidos cardiotónicos - - - - - - - - -Carbohidratos reductores ++ + ++ + ++ + - - + + ++ + ++ + + + ++ +

Fenoles y/o taninos + + + + + + + + + + + + + + + + + +Quinonas - - - + + - + - -

Antocianidinas + - - - - - + - -Flavonoides - - + + + ++ - + - + + +++ + + + + + ++

Principios amargos - - + - - + + - +Mucílagos - - - - - - - - -

Corteza del tallo FrutoMetabolitos

Hojas Corteza del tallo FrutoCabo Cruz Pilón Manzanillo

Hojas Corteza del tallo Fruto Hojas

Metabolites

North of east regionLeaves Bark of the shaft Fruits

DCM EtOH EtOAc DCM EtOH EtOAc DCM EtOH EtOAc

Alkaloids - + - - ++ + - ++ -Coumarins + ++ + ++ ++ - + ++ +Fatty Acid - - - - - - + - -

Resins - - - - - - - - -Triterpenes/Steroids - - - - - - + - -

Saponines* (ultrasonido) - -* - - - - - -* -Saponines* (maceración) - +* - - - - - +* -

Free amino acid - - - - - - - + -Cardiotonic Glycosides - - - - - - - - -

Reducers Carbohydrates - ++ + - + + - ++ ++Phenols/Tanines + +++ +++ + +++ ++ - +++ ++

Quinones - - - - - - - - -Antocianidines - + + - - - - - -

Flavonoids - + - - + - - + -Better compounds - - - - + - - + -

Mucilages - - - - - - - - -

ecophysiological characterization of coccoloba uvifera l.: the case of a salt and drought stress...

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