laboratory of ecophysiological anatomy

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Research topic B) Ecophysiology of mycorrhizal symbioses and associations B1. Application of mycorrhizal inoculations in agriculture production 2 topics: B2. The role of mycorrhizal symbioses and associations in coniferous forest Institute of Botany, AS CR, v.v.i. : Dr. M. Vosátka Mendel University in Brno, Faculty of Horticulture, Lednice: doc. Pokluda, Ing. Nedorost biotechnological company Symbiom: Dr. A. Látr Szent István University (Hungary) – Dr. K. Posta Volcani Center, Israel – prof. Kapulnik Institute of Botany, AS CR, v.v.i. : Dr. Vohník, Mgr. Kohout, T. Lukešová, T. Antl Global Change Research Centre AS CR, v.v.i. -doc. P. Cudlín (former joint projects, COST action) University of Tartu (Estonia) – Dr. Tedersoo University of Lund (Sweden) – prof. Wallander Collaboration: Collaboration : Laboratory of ecophysiological anatomy Department of Experimental Plant Biology, http://kfrserver.natur.cuni.cz Charles University in Prague, Faculty of science

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Page 1: Laboratory of ecophysiological anatomy

Research topic B) Ecophysiology of mycorrhizal symbioses and associations

B1. Application of mycorrhizal inoculations in agriculture production

2 topics:

B2. The role of mycorrhizal symbioses and associations in coniferous forest

• Institute of Botany, AS CR, v.v.i. : Dr. M. Vosátka• Mendel University in Brno, Faculty of Horticulture, Lednice: doc. Pokluda, Ing. Nedorost• biotechnological company Symbiom: Dr. A. Látr

• Szent István University (Hungary) – Dr. K. Posta• Volcani Center, Israel – prof. Kapulnik

•Institute of Botany, AS CR, v.v.i. : Dr. Vohník, Mgr. Kohout, T. Lukešová, T. Antl•Global Change Research Centre AS CR, v.v.i. -doc. P. Cudlín (former joint projects, COST action)

University of Tartu (Estonia) – Dr. Tedersoo• University of Lund (Sweden) – prof. Wallander

Collaboration:

Collaboration :

Laboratory of ecophysiological anatomy

Department of Experimental Plant Biology, http://kfrserver.natur.cuni.cz Charles University in Prague, Faculty of science

Page 2: Laboratory of ecophysiological anatomy

and Dr. Vosátka z IB AS �R:

B2-1. COST OC09057 Development of sustainable production scheme of selected vegetable species on the basis of mycorrhizal biotechnologyPI: doc. Albrechtová

Current research projects:

B2-1. COST OC10058 Roots, ectomycorrhizas, and below ground carbon balance of Norway spruce forests of Central EuropePI: doc. Albrechtová, coinvestigator Dr. M. Vohník

B2-2. GAUK 2011/320311 Role of DSE (Dark Septate Endophytes) in plant community of forest ecosystemPI – Bc. T. Lukešová (supervisor dr. M. Vohník, consultants Mgr. P. Kohout, Doc. Albrechtová)

B2. in collaboration with the Department ofMycorrhizal Symbioses, Institute of Botany :

B1. in collaboration with Symbiom company and

Laboratory of ecophysiological anatomy

Department of Experimental Plant Biology, http://kfrserver.natur.cuni.cz Charles University in Prague, Faculty of science

Research topic B) Ecophysiology of mycorrhizal symbioses and associations

Page 3: Laboratory of ecophysiological anatomy

The goal of the study is to test and adjust optimal cultivation of selected vegetable species in combination with yield increase and improvement of plant nutrition quality (e.g. antioxidat contents, mineral nutrients etc.) using microbial inoculation –

combination of mycorrhizal and saprothropic fungi pre-inoculated on plant biomass.

B2-1. COST OC09057 Development of sustainable production scheme of selected vegetable species on the basis of mycorrhizal biotechnologyPI: doc. Albrechtová, collaboration with Symbiom company – Dr. Látr, consultant Dr. M. Vosátka

B1. Application of mycorrhizal inoculations in agriculture production

Laboratory of ecophysiological anatomy

Department of Experimental Plant Biology, http://kfrserver.natur.cuni.cz Charles University in Prague, Faculty of science

Research topic B) Ecophysiology of mycorrhizal symbioses and associations

Page 4: Laboratory of ecophysiological anatomy

Publications and outputs from last 5 years :

Vosátka, Miroslav; Albrechtová, Jana; Patten, Robert. Mycorrhiza - State of the Art, Genetics and Molecular Biology, Eco-Function, Biotechnology, Eco-Physiology, Structure and Systematics. 3. Heidelberg : Springer, 2008. ISBN 978-35-40788-24-9. The International Market Development for Mycorrhizal Technology. s. 419-438.

Vosátka, Miroslav; Albrechtová, Jana. Floriculture, Ornamental and Plant Biotechnology. 1. Ikenobe, Japonsko : GlobalScience Books, Ltd., 2008. V. ISBN 978-4-903313-12-2. Theoretical aspects and practical uses of mycorrhizal technologyin floriculture and horticulture. s. 466-479.

Vosátka M, Gajdoš J, Kolomý P, Kavková M, Oliveira RS, Franco AR, Sousa NR, Carvalho MF, Castro PML, Albrechtová J.Mycorrhiza Works. 1. Braunschweig : Deutsche Phytomedizinische Gesellschaft, 2008. ISBN 978-3-941261-01-3.Applications of ectomycorrhizal inocula in nursery and field plantings: the importance of inoculum tuning to target conditions. s. 112-124.

Vosátka M, Látr A, Albrechtová J. Mycorrhiza Works. 1. Braunschweig : Deutsche Phytomedizinische Gesellschaft, 2008. ISBN 978-3-941261-01-3. How to apply mycorrhizal inocula in a large-scale and what outcome can be expected in respectto plant growth. s. 323-339.

Albrechtova J, Latr A, Nedorost l, Pokluda R, Posta K, Vosatka M (submitted): Dual inoculation by mycorrhizal and saprotrophic fungi applicable in sustainable cultivation improves the yield and nutritive value of onion. Botany TSWJ

Hernádi I, Sasvári Z, Albrechtova J, Vosatka M, Posta K (under preparation): Field application of commercial arbuscular mycorrhizal (AM) inoculation increases yield of spice pepper (Capsicum annuum L. var. longum cv. Szegedi) and affects indigenous AM fungal community

Laboratory of ecophysiological anatomy

Department of Experimental Plant Biology, http://kfrserver.natur.cuni.cz Charles University in Prague, Faculty of science

Research topic B) Ecophysiology of mycorrhizal symbioses and associations

B1. Application of mycorrhizal inoculations in agriculture production

Page 5: Laboratory of ecophysiological anatomy

Current research projects:

B2. The role of mycorrhizal symbioses and associations in coniferous forest

B2. in collaboration with the Department ofMycorrhizal Symbioses, Institute of Botany :

B2-1. COST OC10058 Roots, ectomycorrhizas, and below ground carbon balance of Norway spruce forests of Central EuropePI: doc. Albrechtová, coinvestigator Dr. M. Vohník

B2-2. GAUK 2011/320311 Role of DSE (Dark Septate Endophytes) in plant community of forest ecosystemPI – Bc. T. Lukešová (supervisor dr. M. Vohník, consultants Mgr. P. Kohout, Doc. Albrechtová)

Laboratory of ecophysiological anatomy

Department of Experimental Plant Biology, http://kfrserver.natur.cuni.cz Charles University in Prague, Faculty of science

Research topic B) Ecophysiology of mycorrhizal symbioses and associations

Page 6: Laboratory of ecophysiological anatomy

CREW – in collaboration with: the Department of Mycorrhizal Symbioses, Institute of Botany :

GAUK 2009/71409 Is biological fitness of invasive Pinus strobus affected by ericaceous plants and their symbiotic fungi? Comparison with Pinus silvestris. PI: Petr Kohout.

Laboratory of ecophysiological anatomy

Department of Experimental Plant Biology, http://kfrserver.natur.cuni.cz Charles University in Prague, Faculty of science

B2. The role of mycorrhizal symbioses and associations in coniferous forest

Page 7: Laboratory of ecophysiological anatomy

Current research projects :

B2-2. GAUK 2011/320311 Role of DSE (Dark Septate Endophytes) in plant community of forest ecosystemPI – Bc. T. Lukešová (supervisor dr. M. Vohník, consultants Mgr. P. Kohout, Doc. Albrechtová)

Laboratory of ecophysiological anatomy

Department of Experimental Plant Biology, http://kfrserver.natur.cuni.cz Charles University in Prague, Faculty of science

Research topic B) Ecophysiology of mycorrhizal symbioses and associations

B2. The role of mycorrhizal symbioses and associations in coniferous forest

• Dark Septate Endophytes are jsou ubiquitous plant root endophytes, theirphysiological impact is not well understood yet. DSE fungi connect with their mycelia root systems of different plants and this inetrconnection may influenceplant nutrition uptake and can mediate interactions between the plants.

What impact have selected representatives of DSE on host plants?

Can DSE mediate interactions between ericoid plants and conivers

within the forest ecosystem?

Page 8: Laboratory of ecophysiological anatomy

B2-1. COST OC10058 Roots, ectomycorrhizas, and below ground carbon balance of Norway spruce forests of Central EuropePI: doc. Albrechtová, spolu�ešitel Dr. M. Vohník

•Interactions of allochthonous plants and mycorrhizal fungi can contribute to an invasive character of those allochtonous mycorrhizal plants depending on 1) diversity of mycorrhizal fungi and 2) different carbon allocation into belowground plant biomass or mycorrhizal structures.

How the invasive specie - Eastern White Pine influence the

autochtonous mycorrhizal communities?

Laboratory of ecophysiological anatomy

Department of Experimental Plant Biology, http://kfrserver.natur.cuni.cz Charles University in Prague, Faculty of science

Research topic B) Ecophysiology of mycorrhizal symbioses and associations

B2. The role of mycorrhizal symbioses and associations in coniferous forest

Current research projects :

Page 9: Laboratory of ecophysiological anatomy

Current outputs and publications:

Kohout P, Sýkorová Z, Bahram M, Hadincová V, Albrechtová J, Tedersoo L & Vohník M (2011)Understorey ericaceous shrubs affect ectomycorrhizal fungal community of the invasive Pinus strobus and native Pinus sylvestris in a pot experiment. Mycorrhiza 21: 403-412.

Tedersoo L, Abarenkov K, Nilsson RH, Schü�ler A, Grelet GA, Kohout P, Oja J, Bonito GM,

Veldre V, Jairus T, Ryberg M, Larsson KH & Kõljalg U (2011) Tidying up GenBank: quality and

metadata annotation of ITS sequences of mycorrhizal fungi. PLoS ONE 6: 9-e24904.

Vohník M & Albrechtová J, The Co-occurrence and Morphological Continuum Between Ericoid

Mycorrhiza and Dark Septate Endophytes in Roots of Six European Rhododendron Species.

Folia Geobotanica (in press)

Kohout P, Sýkorová Z, �tvrtlíková M, Rydlová J, Suda J, Vohník M & Sudová R, Surprising

spectra of root associated fungi in submerged aquatic plants. Accepted in FEMS Microbiology

Ecology

Laboratory of ecophysiological anatomy

Department of Experimental Plant Biology, http://kfrserver.natur.cuni.cz Charles University in Prague, Faculty of science

Research topic B) Ecophysiology of mycorrhizal symbioses and associations

B2. The role of mycorrhizal symbioses and associations in coniferous forest