Download - Methods for studying Adipocyte Lipolysis
Methods for studying Adipocyte Lipolysis
Whole-Body Lipolysis– Ra of glycerol in the bloodstream– Measuring the dilution of tracer-
labeled glycerol in plasma (isotopic dilution)
– Has some possible inconsistencies Regional Lipolysis
– Lipolytic activity can be assessed in individual tissues using arteriovenous methods
– Evaluation of the balance between delivery and release of glycerol across adipose tissue bed.
– Done by measuring arterial & venous glycerol concentrations and adipose tissue blood flow (ATFB)
– Methods were arterial and venous catheterization and microdialysis probes. Schematic diagram of a microdialysis probe and
the method used to collect samples
More Measurement Techniques
Measurements of hormones, metabolite concentrations and blood lactate concentrations were measured by Goto via different methods
– Epi & Ne measured with high performance liquid chromatorograph kits
– Serum GH measured with radioimmunossay kits– Serum Insulin measured with Enzymic immunoassay– Serum FFA & Ketone Body measured with Enzymatic
methods– Serum Glycerol measured with Enzymatic colorimetric– Lactate measured by pricking a fingertip for a blood sample