Methods for studying Adipocyte Lipolysis

Whole-Body Lipolysis– Ra of glycerol in the bloodstream– Measuring the dilution of tracer-

labeled glycerol in plasma (isotopic dilution)

– Has some possible inconsistencies Regional Lipolysis

– Lipolytic activity can be assessed in individual tissues using arteriovenous methods

– Evaluation of the balance between delivery and release of glycerol across adipose tissue bed.

– Done by measuring arterial & venous glycerol concentrations and adipose tissue blood flow (ATFB)

– Methods were arterial and venous catheterization and microdialysis probes. Schematic diagram of a microdialysis probe and

the method used to collect samples

More Measurement Techniques

Measurements of hormones, metabolite concentrations and blood lactate concentrations were measured by Goto via different methods

– Epi & Ne measured with high performance liquid chromatorograph kits

– Serum GH measured with radioimmunossay kits– Serum Insulin measured with Enzymic immunoassay– Serum FFA & Ketone Body measured with Enzymatic

methods– Serum Glycerol measured with Enzymatic colorimetric– Lactate measured by pricking a fingertip for a blood sample