Transcript
Ex. 6: Preparation of Basic Growth Media
Key Words and Concepts• Macronutrients • Micronutrients• Fastidious organisms need lots of ____________
• Solid vs. liquid media; complex vs. defined media
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Vocabulary
• NA vs. NB• TSA vs. TSB
• Complex (undefined) vs. Synthetic (defined) media
• Peptones
Always properly label your plates and tubes!
Today:
Making Media and Pouring Plates
• Prepare 100 ml NB media in 250 ml flask
• Weigh out nutrient broth powder for a 0.8% w/v solution. How much NA do you need ???
• Warm the solution on the hot plate to dissolve but DO NOT boil.
• After removing 5 x 4ml to tubes, how much agar do you add to make a 1.5% agar suspension of the rest ???
Autoclave
121 ºC
15 psi
15 minutes