detection of single red blood cell magnetic property using a highly sensitive gmr-sv biosensor...
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Detection of Single Red Blood Cell Magnetic Property using a Highly Sensitive GMR-SV Biosensor
Sang-Suk Lee, Sang-Hyun ParkKwang-Suo Soh
2006.9.27 CKC Symposium
Contents
Magnetism and Sensitivity New Functional Soft Magnetic Materials Measurement and Resolution
Red Blood Cell Magnetophrosis Oxygen - RBC Magnetic Susceptibility Magnetophoretic Mobilities
Set up of Measurement System Micro Capillary Technology Optical Tweezer Technology
Further Corporation Environment Research Field of Prof. Tony Bland’s Group Future Research Plans
Metals, Spin polarization (P), and Magnetism
Metal : n() = n() ( P = (n()-n())/(n()+ n()) =0 )
Ferromagnetism : ( 0<P <1 )
Half Metals: CrO2, Fe3O4, PtMnSb ( P = 1)
3d 10-x
4f 14-x
Four general types of a magnetism
Type Magnetic moment arrangement
Magnetic Suscepibility
Substance
Ferro-magnetis
m
Ferro-
1 ~ 105
Fe, Co, Ni, NiFe Gd, Dy, Er, Co-Pt
Ferri- Fe-O, Ni-Zn, ferrite
Antiferromagnetism 0 NiO, MnO, Fe2O3
FeMn, IrMn, PtMn
Paramagnetism 10-7 ~ 10-3 Al, Ti, W, Cr, O2
Mn, Pt, N2, Sn
Diamagnetism None -10-5 ~ -10-7 Cu, Ti, W, Cr, O2
Mn, Pt, N2, Sn
Properties of GMR-SV Multilayers
Ta 5 nm
NiFe 4.0 nm
FeMn 7.0 nm
Ta 5 nm
NiFe 10 nm
Cu 2.6 nmNFM (Spacer)
FM (Free Layer)
AFM (Pinning Layer)
FM (Pinned Layer)
MR Ratio (Rap-Rp)/Rp = 4 ~ 9 %
Magneto sensitivity MR/H
Rap
RpRp
M-H curve
Sensing position
M-R curve
Hc
Application of GMR-SV Biosensor
PR(1.3 um)
SiO2(100 nm)
Contact pad (160 nm)
Silicon substrate
SV Sensor
Silica coated magnetic nanoparticles
•The low requirement for sample amount •Easy integration for multianalyte detection on a single chip •Inexpensive and portable devices requiring little or no expertise for their use
Advantage of GMR-SV Biosensor
Replace by RBC
Highly Sensitive Magnetic Films
Ni77Fe14Cu5Mo4 (Conetic film (Mu-metal))
Optimized condition : Hc = 0.055 Oe
Minimized purpose : ~0.055 Oe (predicted values) MS(MR/H) = 50 ~150 %/Oe
One of several hundreds for Hc of NiFe Hc = 5~10 Oe
MS(MR/H) = 0.5 ~1.5 %/Oe Measurement by using SQUID
Sensitivity - nano tesla (10-9 T) => 10-5 Oe NiFe, NiFeCo => 10-2~10-3 Oe NiFeCuMo => 10-4~10-5 Oe (theoretically 10-6)
• Sensor size : 26 m2 • Output : 100 V , Resolution : 100 nT = 10-3 G M = 510-22 emu (erg/G) 5 10-2 B
Sensitivity of GMR/SV Biosensor
Tesla
Bio-magneto signal
ECGEEG
Mag
neti
c fi
eld
measu
rin
g lim
it
Cosmos Magnetic field Eart
h fi
eld
Electric Instrumentsaround field
General & Super-Conductor Magnet
PermanentMagnet
High VoltTransmitter,Transformer,Choke Coil,
Motor
Expectation of a Very High Sensitivity of GMR-SV
The Hemoglobin Properties
Of Red Blood Cell
* RBC : normal adult blood volume = 46 L average number = 45×106/cc circulatory lifetime = 120 days 1 RBC = 3×106 Hemoglobin 1 Hemoglobin = 4 Fe atoms
• Ferrous iron(Fe2+)
Fe2O3
• Binding Oxygen
Molecules
• 2-pair Polypetide Chain
Globin+4 Heme Group
• Ferric iron(Fe3+)
Fe3O4
• Loss of carrier power of oxygen and carbon dioxide
• Blue-green color
oxyhemoglobin deoxyhemoglobinmethemoglobin
Ligand & Light Absorption Hemoglobin and Fe
Paramagnetic Properties
Diamagnetic Properties
1. Capillary magnetophoresis of Human blood cells trapping in a flow system J. of Chromatography A, 2002
Apparatus Results
Red Blood Cell Magnetophoresis-1
2. Red Blood Cell Magnetophrosis Maciej Zborowski et al, Biophysical Journal 84, 2638 (2003)
1)The measured magnetic moments of hemoglobin : its compounds on the relatively high hemoglobin concentr
ation of human erythrocytes
2) Differential migration of these cells was possible if exposed to a high magnetic field (1.40 T).
3) Development of a new technology, cell tracking velocimetry (CTV) the migration velocity of oxy-, deoxy-, and metHb-containing erythrocytes
Red Blood Cell Magnetophoresis-2
Red Blood Cell Magnetic Susceptibilities
Red Blood Cell Magnetophoretic Mobilities
Ring Pattern by Liquid Drop Motion of Nano-particles
Before dropAfter drop :formation of ring pattern
Detection of Magnetic Nanoparticles
Change of Sensing Position by the abrupt Variation of Magnetic Field
Drop point
Before state : max & min signal
Output Sensing Signal Observation of Nanopartices
Capillary Capture Red Blood Cell
Biophysics of cell membranes :Investigation of the changes in the mechanical and rheological properties of blood cells in diabetes
Taken by http://newton.ex.ac.uk/research/biomedical/membranes/
Optical Trapping and Manipulation of Single Cells using Infrared Laser Beams
Set up of System-1
Set up of System-2
Micro-hole Capillary with RBCand Biosensor
26 m2
Pure-RBC
Capillary and Approach to Biosenor
GMR-SV Biosensor
CapillaryRed Blood Cell →
Red Blood Cells←
Micro-capillary Movingand Manipulating Images
Needs and supplememts: Advanced Microscope, CCD Images, Uptaking RBC Techniques
Biological Cell Detection usingFerromagnetic Microbeads {by T. Bland’ Group}
Integrated microfluidic cell with multilayer ring sensors for single magnetic microbead detection {by T. Bland’ Group}
Future Research Plans
Fabrication of high sensitive GMR/SV biosensor Extraction of RBC or Heme-Sanal from Bo
nghan Duct
Nano-bio Lab.Sangji University
< Sept. 2006 Nov. 2007 >
BPL, SNU,CKC Research
< Dec. 2006 Feb. 2007 >
Investigation of single RBC’s and
Hemo-Sanal’s magneto-properties
< Dec. 2006 Feb. 2007 >
Fabrication of a highly sensitive GMR/SV biosensor with conetic film
Extraction of RBC or Hemo-Sanal from Bonghan Duct
Practical use of biosensor andmedical instruments
Set up measuring system,using micro-capillary and optical tweezer
To obtain an analytic value of bio-magnetic moleculessuch as : RBC, Hemo-Sanal, etc Using : (1) Micro-capillary controlling technology (2) Optical tweezer trapping and manipulation