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Detection of Single Red Blood Cell Magnetic Property using a Highly Sensitive GMR-SV Biosensor
Sang-Suk Lee, Sang-Hyun ParkKwang-Suo Soh
2006.9.27 CKC Symposium
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Contents
Magnetism and Sensitivity New Functional Soft Magnetic Materials Measurement and Resolution
Red Blood Cell Magnetophrosis Oxygen - RBC Magnetic Susceptibility Magnetophoretic Mobilities
Set up of Measurement System Micro Capillary Technology Optical Tweezer Technology
Further Corporation Environment Research Field of Prof. Tony Bland’s Group Future Research Plans
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Metals, Spin polarization (P), and Magnetism
Metal : n() = n() ( P = (n()-n())/(n()+ n()) =0 )
Ferromagnetism : ( 0<P <1 )
Half Metals: CrO2, Fe3O4, PtMnSb ( P = 1)
3d 10-x
4f 14-x
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Four general types of a magnetism
Type Magnetic moment arrangement
Magnetic Suscepibility
Substance
Ferro-magnetis
m
Ferro-
1 ~ 105
Fe, Co, Ni, NiFe Gd, Dy, Er, Co-Pt
Ferri- Fe-O, Ni-Zn, ferrite
Antiferromagnetism 0 NiO, MnO, Fe2O3
FeMn, IrMn, PtMn
Paramagnetism 10-7 ~ 10-3 Al, Ti, W, Cr, O2
Mn, Pt, N2, Sn
Diamagnetism None -10-5 ~ -10-7 Cu, Ti, W, Cr, O2
Mn, Pt, N2, Sn
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Properties of GMR-SV Multilayers
Ta 5 nm
NiFe 4.0 nm
FeMn 7.0 nm
Ta 5 nm
NiFe 10 nm
Cu 2.6 nmNFM (Spacer)
FM (Free Layer)
AFM (Pinning Layer)
FM (Pinned Layer)
MR Ratio (Rap-Rp)/Rp = 4 ~ 9 %
Magneto sensitivity MR/H
Rap
RpRp
M-H curve
Sensing position
M-R curve
Hc
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Application of GMR-SV Biosensor
PR(1.3 um)
SiO2(100 nm)
Contact pad (160 nm)
Silicon substrate
SV Sensor
Silica coated magnetic nanoparticles
•The low requirement for sample amount •Easy integration for multianalyte detection on a single chip •Inexpensive and portable devices requiring little or no expertise for their use
Advantage of GMR-SV Biosensor
Replace by RBC
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Highly Sensitive Magnetic Films
Ni77Fe14Cu5Mo4 (Conetic film (Mu-metal))
Optimized condition : Hc = 0.055 Oe
Minimized purpose : ~0.055 Oe (predicted values) MS(MR/H) = 50 ~150 %/Oe
One of several hundreds for Hc of NiFe Hc = 5~10 Oe
MS(MR/H) = 0.5 ~1.5 %/Oe Measurement by using SQUID
Sensitivity - nano tesla (10-9 T) => 10-5 Oe NiFe, NiFeCo => 10-2~10-3 Oe NiFeCuMo => 10-4~10-5 Oe (theoretically 10-6)
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• Sensor size : 26 m2 • Output : 100 V , Resolution : 100 nT = 10-3 G M = 510-22 emu (erg/G) 5 10-2 B
Sensitivity of GMR/SV Biosensor
Tesla
Bio-magneto signal
ECGEEG
Mag
neti
c fi
eld
measu
rin
g lim
it
Cosmos Magnetic field Eart
h fi
eld
Electric Instrumentsaround field
General & Super-Conductor Magnet
PermanentMagnet
High VoltTransmitter,Transformer,Choke Coil,
Motor
Expectation of a Very High Sensitivity of GMR-SV
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The Hemoglobin Properties
Of Red Blood Cell
* RBC : normal adult blood volume = 46 L average number = 45×106/cc circulatory lifetime = 120 days 1 RBC = 3×106 Hemoglobin 1 Hemoglobin = 4 Fe atoms
• Ferrous iron(Fe2+)
Fe2O3
• Binding Oxygen
Molecules
• 2-pair Polypetide Chain
Globin+4 Heme Group
• Ferric iron(Fe3+)
Fe3O4
• Loss of carrier power of oxygen and carbon dioxide
• Blue-green color
oxyhemoglobin deoxyhemoglobinmethemoglobin
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Ligand & Light Absorption Hemoglobin and Fe
Paramagnetic Properties
Diamagnetic Properties
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1. Capillary magnetophoresis of Human blood cells trapping in a flow system J. of Chromatography A, 2002
Apparatus Results
Red Blood Cell Magnetophoresis-1
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2. Red Blood Cell Magnetophrosis Maciej Zborowski et al, Biophysical Journal 84, 2638 (2003)
1)The measured magnetic moments of hemoglobin : its compounds on the relatively high hemoglobin concentr
ation of human erythrocytes
2) Differential migration of these cells was possible if exposed to a high magnetic field (1.40 T).
3) Development of a new technology, cell tracking velocimetry (CTV) the migration velocity of oxy-, deoxy-, and metHb-containing erythrocytes
Red Blood Cell Magnetophoresis-2
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Red Blood Cell Magnetic Susceptibilities
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Red Blood Cell Magnetophoretic Mobilities
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Ring Pattern by Liquid Drop Motion of Nano-particles
Before dropAfter drop :formation of ring pattern
Detection of Magnetic Nanoparticles
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Change of Sensing Position by the abrupt Variation of Magnetic Field
Drop point
Before state : max & min signal
Output Sensing Signal Observation of Nanopartices
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Capillary Capture Red Blood Cell
Biophysics of cell membranes :Investigation of the changes in the mechanical and rheological properties of blood cells in diabetes
Taken by http://newton.ex.ac.uk/research/biomedical/membranes/
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Optical Trapping and Manipulation of Single Cells using Infrared Laser Beams
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Set up of System-1
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Set up of System-2
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Micro-hole Capillary with RBCand Biosensor
26 m2
Pure-RBC
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Capillary and Approach to Biosenor
GMR-SV Biosensor
CapillaryRed Blood Cell →
Red Blood Cells←
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Micro-capillary Movingand Manipulating Images
Needs and supplememts: Advanced Microscope, CCD Images, Uptaking RBC Techniques
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Biological Cell Detection usingFerromagnetic Microbeads {by T. Bland’ Group}
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Integrated microfluidic cell with multilayer ring sensors for single magnetic microbead detection {by T. Bland’ Group}
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Future Research Plans
Fabrication of high sensitive GMR/SV biosensor Extraction of RBC or Heme-Sanal from Bo
nghan Duct
Nano-bio Lab.Sangji University
< Sept. 2006 Nov. 2007 >
BPL, SNU,CKC Research
< Dec. 2006 Feb. 2007 >
Investigation of single RBC’s and
Hemo-Sanal’s magneto-properties
< Dec. 2006 Feb. 2007 >
Fabrication of a highly sensitive GMR/SV biosensor with conetic film
Extraction of RBC or Hemo-Sanal from Bonghan Duct
Practical use of biosensor andmedical instruments
Set up measuring system,using micro-capillary and optical tweezer
To obtain an analytic value of bio-magnetic moleculessuch as : RBC, Hemo-Sanal, etc Using : (1) Micro-capillary controlling technology (2) Optical tweezer trapping and manipulation