control of anthracnose of banana (kluai khai) by the biofumigant fungus muscodor albus strain mfc2
TRANSCRIPT
Control of Anthracnose of banana (Kluai Khai) by the biofumigant fungus Muscodor alb
us strain MFC2Kusuma Potchana
Worraya Prompangta
Chomporn RaksakiettisakAdviser Miss Aree Sakyim
Purposes• To test the efficiency of MFC2 to inhibit the
anthracnose pathogen in banana. • To find the proper nutrient for MFC2Research Boundary• Testing MFC2 with two pathogen ;
Colletotrichum capsici and Colletotrichum gloeosporioides
ระยะเวลาที่ทำาการศึกษา • Twelve months (November 2006 - November 2007)
Term of Research
Laboratory
• Biotechnological laboratoy of Mahidol University
• Biological laboratory of Mahidol Wittayanusorn School
Method
• Equipment and Substances •Colletotrichum capsici •Colletotrichum gloeosporioides•Muscodor albus strain MFC2•Autoclave•Larminar flow•Pasteur pipettes
วธิีดำาเนนิการทดลองMethod
• Part 1 Grow MFC2 on PDA
Do hyphal tip with MFC2
Grow MFC2 for 10 -14 days
Method
•Make nutrients for growing MFC2
Nutrient 1 Nutrient 2
Brown rice 15 grams
millet 35 grams
Bran 5 grams water 5 cm2
Method
millet 50 grams
Bran 5 grams water 5 cm2
• Part 2 Grow Pathogens
Method
• Part 3 Test the efficiency of MFC2 to inhibit the anthracnose pathogen in banana on PDA
10 day control7 day
5 day
Method
• Part 4 Test the efficiency of MFC2 to inhibit the anthracnose pathogen in banana on nutrient 1 and 2
7 day 7 day
Control
Method
Results
ResultsTable 1 Test on PDA
inhibitinhibitPathogens after 5 day
75.3Control1.833.67Average2.73.631.63.721.23.71
C.capsiciC.gloeosporioid
es
Diameter (cm)Plate
• Table 2 Test on nutrient 1
InhibitInhibitPathogen after 5 days
4.44.4Control2.170.63.032.1
5Average
2.514.21.25
330.412.8210.43.92.41
No PDAPDANo PDA
PDA
C.capsiciC.gloeosporioi
des
Diameter (cm)Plate
Results
• Table 3 Test on nutrient 3
inhibitInhibitPathogen after 5 days
4.44.4Control2.171.73.062.0
3Average
3.41.53132.73.21.9220.40.44.33.11No PDA
PDANo PDA
PDA
C.capsiciC.gloeospoiroi
des
Diameter (cm)Plate
Results
• Muscodor albus Strain MFC2 growed in two different nutrients in order to control two anthracnose pathogens of banana ;Colletotrichum capsici and Colletotrichum gloeosporioides can inhibit the two pathogens but does not make any difference. Considered with the quantity of the nutrients, MFC2 growed on nutrient mixed with MFC2 has more efficiency to control the pathogens than another which is growed without PDA.
Results
Conclusions and Analysis
การวิเคราะห์คา่ความแตกต่าง
Conclusions and Analysis
• The nutrient ; water, bran, brown rice and millet, mixed with PDA can grow the MFC2 well and costs low. So this is another way to grow MFC2 to inhibit the two anthracnose pathogens of banana; Colletotrichum capsici and Colletotrichum gloeosporioides.
Advice
การวิเคราะห์คา่ความแตกต่าง
• Test with banana, let farmer use and assess.
• Find the proper temperature for growing MFC2.
• Ezra, D., W.M. Hess and G.A. Strobel. 2004. New endophytic isolates of Muscodor albus, a volatile-antibiotic-producing fungus. Microbiology. 150: 4023–4031.
• Freeman, S., Y. Nizami, S. Dotan, S. Even and T. Sando. 1997. Control of Collectotrichum acutatum in strawberry under laboratory, greenhouse, and field conditions. Plant Disease July 1997: 749-752.
• Kuo, K.C. 2000. Sensitivity of Mango Anthracnose Pathogen, Colletotrichumgloeosporioides, to the Fungicide Prochloraz. Proc. Natl. Sci. Counc. ROC(B) vol. 25 : 174-179.
• Mercier, J. and J.I. Jiménez. 2003. Control of fungal decay of apples and peaches by the biofumigant fungus Muscodor albus. Postharvest Biology and Technology. 31: 1–8.
Reference
• Strobel, G.A., E. Dirkse, J. Sears and C. Markworth. 2001. Volatile antimicrobials from Muscodor albus, a novel endophytic fungus. Microbiology. 147: 2943–2950.
• Sutton, B.C. 1980. The Coelomycetes, Fungi Imperfecti with Pycnidia, Acervuli and Stromata. Kew Surrey, England.