control of anthracnose of banana (kluai khai) by the biofumigant fungus muscodor albus strain mfc2

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Control of Anthracnose o f banana (Kluai Khai) by the biofumigant fungus M uscodor albus strain MFC2 Kusuma Potchana Worraya Prompangta Chomporn Raksakiettisak Adviser Miss Aree S

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Page 1: Control of Anthracnose of banana (Kluai Khai) by the biofumigant fungus Muscodor albus strain MFC2

Control of Anthracnose of banana (Kluai Khai ) by the

biofumigant fungus Musco dor albus strain MFC2

Kusuma PotchanaWorraya

PrompangtaChomporn

RaksakiettisakAdviser Miss Aree Sakyim

Page 2: Control of Anthracnose of banana (Kluai Khai) by the biofumigant fungus Muscodor albus strain MFC2

Purposes• To test the efficiency of MFC2 to inhibit

the anthracnose pathogen in banana. • To find the proper nutrient for MFC2Research Boundary• Testing MFC2 with two pathogen ;

Colletotrichum capsici and Colletotrichum gloeosporioidesระยะเวลาที่�ที่�าการ

ศึ�กษา • Twelve months (November 2006 - November 2007)

Term of Research

Page 3: Control of Anthracnose of banana (Kluai Khai) by the biofumigant fungus Muscodor albus strain MFC2

Laboratory

• Biotechnological laboratoy of Mahidol University• Biological laboratory of Mahidol Wittayanusorn

School

Page 4: Control of Anthracnose of banana (Kluai Khai) by the biofumigant fungus Muscodor albus strain MFC2

Method

Page 5: Control of Anthracnose of banana (Kluai Khai) by the biofumigant fungus Muscodor albus strain MFC2

• Equipment and Substances •Colletotrichum capsici •Colletotrichum gloeosporioides•Muscodor albus strain MFC2•Autoclave• Larminar flow• Pasteur pipettes

ว�ธีดำ�าเนิ�นิการที่ดำลองMethod

Page 6: Control of Anthracnose of banana (Kluai Khai) by the biofumigant fungus Muscodor albus strain MFC2

• Part 1 Grow MFC2 on PDA

Do hyphal tip with MFC2

Grow MFC2 for 10 -14 days

Method

Page 7: Control of Anthracnose of banana (Kluai Khai) by the biofumigant fungus Muscodor albus strain MFC2

•Make nutrients for growing MFC2

Nutrient 1 Nutrient 2

Brown rice 15 grams

millet 35 grams

Bran 5 grams water 5 cm2

Method

millet 50 grams

Bran 5 grams water 5 cm2

Page 8: Control of Anthracnose of banana (Kluai Khai) by the biofumigant fungus Muscodor albus strain MFC2

• Part 2 Grow Pathogens

Method

Page 9: Control of Anthracnose of banana (Kluai Khai) by the biofumigant fungus Muscodor albus strain MFC2

• Part 3 Test the efficiency of MFC2 to inhibit the anthracnose pathogen in banana on PDA

10 day control

7 day

5 day

Method

Page 10: Control of Anthracnose of banana (Kluai Khai) by the biofumigant fungus Muscodor albus strain MFC2

• Part 4 Test the efficiency of MFC2 to inhibit the anthracnose pathogen in banana on nutrient 1 and 2

7 day 7 day

ชุ�ดควบค�ม

Method

Page 11: Control of Anthracnose of banana (Kluai Khai) by the biofumigant fungus Muscodor albus strain MFC2

Results

Page 12: Control of Anthracnose of banana (Kluai Khai) by the biofumigant fungus Muscodor albus strain MFC2

Results

Table 1 Test on PDA

Plate Diameter (cm)

C.gloeosporioides

C.capsici

1 3.7 1.2

2 3.7 1.6

3 3.6 2.7

Average 3.67 1.83

Control 5.3 7

Pathogens after 5

day

inhibit inhibit

Page 13: Control of Anthracnose of banana (Kluai Khai) by the biofumigant fungus Muscodor albus strain MFC2

• Table 2 Test on nutrient 1

Plate Diameter (cm)

C.gloeosporioides

C.capsici

PDA

NoPDA

PDA NoPDA

1 2.4 3.9 0.4 1

2 2.8 1 0.4 3

3 1.25

4.2 1 2.5

Average

2.15

3.03 0.6 2.17

Control 4.4 4.4

Pathogen after 5 days

Inhibit Inhibit

Results

Page 14: Control of Anthracnose of banana (Kluai Khai) by the biofumigant fungus Muscodor albus strain MFC2

• Table 3 Test on nutrient 3 Plate Diameter (cm)

C.gloeospoiroides

C.capsici

PDA

NoPDA

PDA NoPDA

1 3.1 4.3 0.4 0.4

2 2 1.9 3.2 2.7

3 1 3 1.5 3.4

Average

2.03

3.06 1.7 2.17

Control 4.4 4.4

Pathogen after 5 days

Inhibit inhibit

Results

Page 15: Control of Anthracnose of banana (Kluai Khai) by the biofumigant fungus Muscodor albus strain MFC2

• Muscodor albus Strain 2MFC growed in two different nutrients in order to control two anthracnose pathogens of banana ;Colletotri

chum capsici and Colletotrichum gloeosporioides can inhibit the two pathogens but does not make any difference. Considered with the quantity of the nutrients, MFC2 growed on nutrient mixed with MFC2 has more efficiency to control the pathogens than another which is growed without PDA.

Results

Page 16: Control of Anthracnose of banana (Kluai Khai) by the biofumigant fungus Muscodor albus strain MFC2

Conclusions and Analysis

Page 17: Control of Anthracnose of banana (Kluai Khai) by the biofumigant fungus Muscodor albus strain MFC2

การว�เคราะห์�ค�าความแตกต�าง

Conclusions and Analysis

• The nutrient ; water, bran, brown rice and millet, mixed with PDA can grow the MFC2 well and costs low. So this is another way to grow MFC2 to inhibit the two anthracnose pathogens of banana;

Colletotrichum capsici and Colletotrichum gloeosporioides.

Page 18: Control of Anthracnose of banana (Kluai Khai) by the biofumigant fungus Muscodor albus strain MFC2

Advice

การว�เคราะห์�ค�าความแตกต�าง

• Test with banana, let farmer use and assess.

• Find the proper temperature for growing MFC2.

Page 19: Control of Anthracnose of banana (Kluai Khai) by the biofumigant fungus Muscodor albus strain MFC2

• 2004Ezra, D., W.M. Hess and G.A. Strobel. . New endophytic is MMMMMMMM MMMMMM M MMMMMMMM-MMMMMMMMMM-MMMMMMMMM MMMMMMM,. Microbiology . 150: 4023–4031.

• Freeman, S., Y. Nizami, S. Dotan, S. EvenandT. Sando. 1997. Cont r ol of Collectotrichum acutatum MMMMM MMMMMMMMMMM MMMMMMMMMMM MMM MMMMM M,,

. Plant Disease -1997: 749 752.• MM MMMMM MMMMMMMMMMM MMMMMMMMM , .. 2000. , Colletotrichumgloeosp

orioides , to the Fungicide Prochloraz. Proc. Natl. Sci. Counc. ROC(B) -25 174179vol. : .

• 2003Mercier, J. and J.I. Jiménez. . Control of fungal decay of ap pl es and peaches by t he bi of umi gant f ungus Muscodor albus. Po

stharvest Biology and Technology. 31 1 8: – .

Reference

• 200Strobel, G.A., E. Dirkse, J. Sears and C. Markworth. 1 . Volatile antimicrobials from Muscodor albus M MMMMM MMM,

MMMMMMM . Microbiology 147 2943 2950. : – .• , .. 1980. The Coelomycetes, Fungi Imperfecti with Pyc

nidia, Acervuli and Stromata MMMMMMMM . ,.