continuous administration of the 5-ht agonist, f 13640...
TRANSCRIPT
JPET #50286
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Continuous administration of the 5-HT1A agonist, F 13640 attenuates
allodynia-like behavior in a rat model of trigeminal neuropathic pain
Kristof Deseure, Wouter Koek, Hugo Adriaensen and Francis C. Colpaert
Laboratory of Anesthesiology, University of Antwerp, Universiteitsplein 1, B-
2610 Antwerp, Belgium (K.D., H.A.); Centre de Recherche Pierre Fabre, 17
avenue Jean Moulin, 81106 Castres Cédex, France (W.K., F.C.C.)
Copyright 2003 by the American Society for Pharmacology and Experimental Therapeutics.
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Running title: 5-HT1A receptor activation relieves mechanical allodynia
Corresponding author: Kristof Deseure
Universiteit Antwerpen
Labo Anesthesiologie S4
Universiteitsplein 1
B-2610 Antwerpen
Belgium
tel: +32 3 820 2561
fax: : +32 3 820 2501
e-mail: [email protected]
26 text pages
0 tables
6 figures
38 refs.
237 words in the Abstract
1208 words in the Introduction
1898 words in the Discussion
IoN-CCI : chronic constriction injury of the infraorbital nerve
Recommended section: Behavioral Pharmacology
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Abstract
F 13640 is a recently discovered high-efficacy 5-HT1A receptor agonist that produces
central analgesia through the neuroadaptive mechanisms of inverse tolerance and
co-operation. In a rat model of trigeminal neuropathic pain, the chronic constriction
injury of the infraorbital nerve causes allodynia-like behavior that develops within two
weeks and remains stable thereafter. We report that, early after surgery during which
time allodynia develops, the continuous two-week infusion of 0.63 mg/day of F 13640,
inhibited the allodynia-like behavior, whereas 5 mg/day of morphine showed no
significant effect. When F 13640 infusion was initiated late after surgery, when
allodynia was well established, it produced an anti-allodynic effect that was apparent
during the entire infusion period. In contrast, morphine infusion caused an initially
marked anti-allodynic effect to which tolerance developed within the two-week
infusion period. The GABA-B receptor agonist baclofen (1.06 mg/day) that has a
recognized usefulness in the treatment of trigeminal neuralgia, demonstrated
effectiveness in both conditions. The data are consistent with a theory of nociceptive
signal transduction, as well as with earlier data, in demonstrating the neuroadaptive
mechanisms of inverse tolerance and cooperation. That is, in contrast with morphine,
the anti-allodynic effect induced by 5-HT1A receptor activation does not decay, but, if
anything, grows with chronicity. Also, 5-HT1A receptor activation appeared to co-
operate with nociceptive stimulation in, paradoxically, inducing an anti-allodynic
effect. The data presented here suggest that F 13640 may perhaps offer a lasting
treatment of trigeminal neuralgia.
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The 5-HT1A receptor agonist, F 13640 has been shown (Colpaert et al., 2002) to
display a unique pattern of actions that is best understood in terms of a new theory of
the mechanisms of pain and analgesia. This concept of signal transduction in
nociceptive systems (Colpaert, 1978, 1996; Colpaert and Frégnac, 2001) specifies
that any input to such systems causes not a single effect but two effects that are
bidirectional, or opposite in sign. Thus, morphine produces both analgesia as a so-
called 1st order effect, and also hyperalgesia as a 2nd order effect. Upon chronic
exposure to morphine, the 2nd order hyperalgesia grows and neutralizes the 1st order
analgesia (i.e., development of tolerance); the concept thus provides an account of
the neuroadaptive actions that dynamically ensue upon µ-opioid receptor activation
(Colpaert, 1996). Also according to this concept, nociceptive stimulation should
similarly produce dual, bidirectional effects which should amount to the mirror
opposite of those produced by morphine.
We have recently discovered (Colpaert et al., 2002) that high-efficacy
activation of 5-HT1A receptors constitutes a molecular mechanism that mimics the
central actions of nociceptive stimulation. A single injection of the selective, high-
efficacy 5-HT1A agonist, F 13640 produced dual and bidirectional, hyper- and hypo-
algesic effects on the vocalization threshold to mechanical stimulation in normal rats.
These effects were shown to be related to the 5-HT1A receptor activation that F 13640
produces in a selective manner (Colpaert et al., 2002). Upon repeated injection, F
13640 produced an initial hyperalgesia that then decayed while the hypo-algesia
became amplified, thus demonstrating the development of inverse tolerance.
Continuous infusion of F 13640 produced an antinociceptive effect in rodent models
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of chronic nociceptive pain and of chronic allodynia that surpassed that of morphine
and of other mechanisms of central analgesia. These data thus identify large-
amplitude 5-HT1A receptor activation as a new molecular mechanism of analgesia,
the neuroadaptive mechanisms of which consist, remarkably, of inverse tolerance
and, also, of co-operation with nociceptive stimulation in producing analgesia
(Colpaert et al., 2002). Indeed, in as much as F 13640 mimics the central effects of
nociceptive stimulation, its effects should add to those of any such stimulation in
producing analgesia (Colpaert, 1996). Thus, while 0.63 mg/kg of F 13640 produced
marked hyperalgesia in normal rats 15 min upon its intraperitoneal (i.p.) injection, the
compound produced profound analgesia in rats that were exposed to the severe,
tonic nociception induced by the inoculation of 50 µl of 2.5 % formaldehyde into the
hind paw plantar surface (Colpaert et al., 2002).
The notion that 5-HT1A receptor activation may offer a new approach to the
treatment of pain is consistent with the long-standing recognition of serotonergic
mechanisms in pain control (for review, see: Cesselin et al., 1994; Hamon and
Bourgoin, 1999). Both supraspinally and spinally projecting pathways are involved;
importantly, pathways originating in the brainstem and projecting to the spinal cord
dorsal horn are thought to mediate an endogenous, serotonergic pain-suppressing
system (Villanueva and Le Bars, 1995). While many different 5-HT receptors may be
implicated (Barnes and Sharp, 1999; Millan, 1995), considerable evidence points to
an important role of 5-HT1A receptors (Hamon and Bourgoin, 1999). For example, 5-
HT1A receptors likely mediate the inhibitory effect, on spinal pain transmission, of the
descending 5-HT pathway that originates in the nucleus raphe magnus (Zemlan et al.,
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1994) as well as the opioid-induced bulbospinal inhibition of spinal withdrawal reflexes
(Clarke and Ward, 2000). Also, 5-HT1A receptor activation applied directly onto the
spinal cord inhibits the activity of spinal wide dynamic range neurons after repeated
electrical stimulation (Gjerstad et al., 1996).
In spite of the availability of opioids, re-uptake inhibitors of noradrenaline and
serotonin, and anticonvulsants, the treatment of neuropathic pains in patients, and of
trigeminal neuralgia in particular, remains largely unsatisfactory (Ollat and Cesaro,
1995; Sindrup and Jensen, 1999, 2002; Attal, 2001). In a rat model of trigeminal
neuropathic pain, in which a chronic constriction injury of the infraorbital nerve (IoN-
CCI) causes von Frey hair stimulation of the skin area within the IoN territory to elicit
allodynia-like behavior (Vos et al., 1994), it was shown that the GABA-B receptor
agonist baclofen and the novel anticonvulsant gabapentin, but not the anticonvulsants
carbamazepine or lamotrigine, morphine, or the tricylic antidepressants amitriptyline
and clomipramine, partially attenuate the allodynia-like behavior (Christensen et al.,
2001; Idänpään-Heikkilä and Guilbaud, 1999). The combination of the glycine/NMDA
receptor antagonist (+)-HA966 ((+)-1-hydroxy-3-aminopyrrolidine-2-one) and
morphine was also found to dose-dependently increase mechanical response
thresholds (Christensen et al., 1999). A recent study reported that antimigraine 5-
HT1B/1D receptor agonists also partially attenuate the allynia-like behavior (Kayser et
al., 2002). Importantly, the single i.p. injection of 0.63 mg/kg of F 13640 fully
normalized allodynic hyperresponsiveness in this model (Deseure et al., 2002). The
magnitude of F 13640’s effects was similar to that of 5 mg/kg of baclofen, while as
high a dose as 10 mg/kg of morphine was required to achieve an equivalent anti-
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allodynic effect. A 4-fold higher dose of baclofen, but not of F 13640, depressed
response scores in a non-specific manner (i.e., below pre-lesion levels, both ipsi- and
contralaterally; Deseure et al., 2002). These data suggest that F 13640 may perhaps
offer an effective treatment of trigeminal neuralgia.
Trigeminal neuralgia being a chronic pathology, it is essential that potential
treatments be evaluated while being implemented in a chronic fashion to determine
their long-term efficacy. This is all the more pertinent as the co-operation between
nociceptive stimulation and 5-HT1A receptor activation in producing analgesia must be
expected (Colpaert, 1996) to be both duration- and intensity-dependent. This
duration-dependence of F 13640’s anti-allodynic effect was studied here by
monitoring responsiveness to von Frey hair stimulation in IoN-CCI rats throughout a
two-week period. The intensity-dependence was studied by comparing F 13640’s
effects with those of another 5-HT1A agonist, F 13714 (Koek et al., 2001), which
activates 5-HT1A receptors to a smaller extent than that achieved with F 13640
(Colpaert et al., 2002). Note that both F 13714 (Koek et al., 2001) and, in particular, F
13640 are highly selective for 5-HT1A receptors; F 13640 binds to rat and human 5-
HT1A receptors with a pKi of 9.1 and 9.5, respectively, while its IC50 exceeds 1000 nM
with the 46 other receptors and ion channels for which its activity has been
determined (Colpaert et al., 2002). The latter included various 5-HT receptor types
and subtypes (i.e., 5-HT1B , 5-HT2A , 5-HT2C , 5-HT3 , 5-HT4 , h5-ht6 , 5-HT7
receptors), as well as various dopaminergic, noradrenergic, GABA-ergic and opioid
receptors. These compounds were compared to two reference treatments, i.e.,
morphine and baclofen, which are effective upon acute administration in the IoN-CCI
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model, but have not been studied upon chronic administration (Deseure et al., 2002).
Finally, allodynic hyperresponsiveness in the IoN-CCI model develops progressively
over the first two weeks after the nerve injury, to reach an asymptote that persists for
at least seven weeks (Vos et al., 1994). Drug effects here were examined early after
surgery, at the time when allodynia developed, as well as late after surgery, after it
had reached asymptote. For the reasons outlined above, the transduction concept
would suggest that the analgesic effects of both morphine and of 5-HT1A agonists
should be more prominent at a time when persistent allodynia had been established
then when this allodynia had not yet fully developed.
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Materials and methods
Subjects
Male Sprague-Dawley rats (Charles River, n = 208 weighing 220-240g on arrival)
were used. Rats were housed in solid bottom polycarbonate cages in a colony room
(38 ± 9% R.H.; 21 ± 1°C). Water and food were available at libitum. Rats were kept
under a reversed 12:12 hr dark/light cycle (lights on at 20 h) and were allowed to
acclimate to the housing facilities for at least 12 days before pre-operative testing.
Animals were treated and cared for according to the guidelines of the Committee for
Research and Ethical Issues of IASP (1983).
ION-CCI model: study Design
Two experiments were conducted. In both experiments, rats were implanted with
osmotic pumps to examine the effects of chronic administration of four different drugs
(see Drugs and doses) on allodynia-like behavior following chronic constriction injury
of the infraorbital nerve (IoN-CCI). Pumps contained one of four different compounds
or saline and released their content at a constant rate (i.e., 0.12 ml/day) during a
period of 14 days.
In the first experiment (n=84 in total; n=12 in each of the seven experimental
groups), we examined the effects of the drug treatments on the development of
allodynia during the first two weeks after surgery. Rats were implanted with osmotic
pumps immediately after IoN-CCI surgery and treatment effects were studied on days
4, 6, 8, 11, 13 and 15 after surgery. To evaluate the effect of IoN-CCI surgery, saline-
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treated rats were compared with sham-operated rats, which were also implanted with
pumps containing saline. To examine the persistence of the effects of the drug
treatments, rats were also tested six days following the removal of osmotic pumps
(i.e., on day 21).
In the second experiment (n=68 in total; n=9-12 in each of the six experimental
groups), we examined the effects of the drug treatments on allodynia that was already
established, i.e. three to five weeks after IoN-CCI ligation. First, the effect of IoN-CCI
surgery was evaluated by comparing the pre-surgery data with post-surgery data; rats
were tested one day before surgery and 6, 11, 16, 21 and 24 days thereafter.
Following testing on day 24, rats were implanted with the pumps. During the following
two weeks, treatment effects were examined on days 27, 29, 31, 34, 36 and 38 after
surgery.
Surgery
The unilateral ligation of the IoN was performed as described elsewhere (Vos et al.,
1994). Rats were anaesthetized with pentobarbital (60 mg/kg, i.p.) and treated with
atropine (0.1 mg/kg, i.p.). Surgery was performed under direct visual control using a
Zeiss operation microscope (x10-25). The head of the rat was fixed in a stereotaxic
frame and a mid-line scalp incision was made, exposing skull and nasal bone. The
infraorbital part of the IoN was exposed using a surgical procedure similar to that
described previously (Gregg, 1973; Jacquin and Zeigler, 1983). The edge of the orbit,
formed by the maxillary, frontal, lacrimal and zygomatic bones, was dissected free.
To give access to the IoN, the orbital contents were gently deflected with a cotton-
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tipped wooden rod. The IoN was dissected free at its most rostral extent in the orbital
cavity, just caudal to the infraorbital foramen. Two chromic catgut ligatures (5-0;
Ethicon, Johnson & Johnson, Belgium) were loosely tied around the IoN (2 mm
apart). To obtain the desired degree of constriction, a criterion proposed by Bennet
and Xie (1988) was applied: the ligatures reduced the diameter of the nerve by a just
noticeable amount and slowed, but did not interrupt the circulation through the
superficial vasculature. The scalp incision was closed using polyester sutures (4/0;
Ethicon, Johnson & Johnson, Belgium). In sham operated rats, the IoN was exposed
on one side using the exact same procedure, but the exposed IoN was not ligated.
Implantation of Alzet osmotic pumps (model 2ML2, nominal pump rate: 5 µl/h;
Alza Corporation, Palo Alto, California, USA) was also performed under anesthesia.
In experiment one, pumps were implanted immediately after IoN-CCI and while the
rats were still anesthetized. In experiment two, rats were implanted with the pumps 24
days after surgery. They were placed in a cage under 4% halothane (Fluothane®,
Zeneca, Belgium). When the rats were fully anesthetized (after ca. 3-4 minutes), they
were taken out of the cage, shaved and placed under a mask with 2.5% halothane.
The shaved dorsal area was disinfected with Hibitane® (chlorhexidine 0.5% in alcohol
70°). An incision of approximately 2 cm was made in the skin, a subcutaneous pocket
was created with a hemostatic forceps and 0.1 ml of antibiotic (Pentrexyl®, Na
ampicillin, 500 mg in 5 ml saline, S.A. Bristol-Myers Squibb) was released into the
pocket with a sterile syringe (1 ml luer). The pump was inserted subcutaneously with
the opening towards the head of the rat. Finally, the incision was closed with four or
five stainless steel staples (Disposable skin stapler with auto-release Appose 35
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Regular, Sherwood – Davis & Geck) and sprayed with Aluspray®. Removal of the
osmotic pumps was performed under halothane anesthesia as described above. The
staples were removed from the skin using a hemostatic forceps and an incision of
approximately 2 cm was made in the skin, the pump was removed from the
subcutaneous pocket and the incision was again closed with four or five staples and
sprayed with Aluspray® (Vétoquinol, France).
Behavioral testing
Rats were habituated to the test procedure on pre-operative days -7, -5 and -3 in the
first experiment and on pre-operative days -7 and -5 in the second experiment.
Baseline data was obtained one day before surgery. Habituation and testing were
conducted in a darkened room (light provided by a 60W red light bulb suspended 1 m
above the test area) with a 45dB background noise, by an observer that was blind to
the drugs that were used. Rats were place in a small transparent cage (l x w x h:
24x14x17 cm). As elsewhere (Vos et al., 1994), a graded series of five von Frey hairs
(Pressure Aesthesiometer®, Stoelting Co, Chicago, IL) were used. The force required
to bend the hairs was 0.015 g, 0.127 g, 0.217 g, 0.745 g and 2.150 g, respectively.
The stimuli were applied within the IoN territory, near the centre of the vibrissal pad,
on the hairy skin surrounding the mystacial vibrissae. This area was stimulated
unilaterally before surgery, and on both sides of the face after surgery, i.e. ipsilateral
and contralateral to the side of surgery. Stimuli were applied in an ascending order of
intensity. The ipsilateral and contralateral side were stimulated in a randomized order
for each stimulus intensity, within each subject. The scoring system described by Vos
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et al. (1994) was used to evaluate the response of the rats to the stimulation. The
response was observed to belong to one of the following response categories: (score
0) no response; (score 1) detection = the rat turns the head toward the stimulating
object and the stimulus object is then explored; (score 2) withdrawal reaction = the rat
turns the head slowly away or pulls it briskly backward when the stimulation is
applied, sometimes a single face wipe ipsilateral to the stimulated area occurs; (score
3) escape/attack = the rat avoids further contact with the stimulus object, either
passively by moving its body away from the stimulating object to assume a crouching
position against the cage wall, or actively by attacking the stimulus object, making
biting and grabbing movements; (score 4) asymmetric face grooming = the rat
displays an uninterrupted series of at least three face-wash strokes directed toward
the stimulated facial area.
Drugs and doses
The following drugs were used: F 13640 (0.63 mg/day; (3-chloro-4-fluoro-phenyl)-[4-
fluoro-4-{[(5-methyl-pyridin-2-ylmethyl)-amino]-methyl}piperidin-1-yl]-methadone) and
F 13714 glycolate (0.16 mg/day; 3-chloro-4-fluorophenyl-(4-fluoro-4-[[(5-methyl-6-
methylamino-pyridin-2-ylmethyl)-amino]-methyl]-piperidin-1-yl-methanone) (Pierre
Fabre, Castres, France), morphine hydrochloride (5 mg/day) (Belgapio, Louvain-La-
Neuve, Belgium) and baclofen (1.06 mg/day) (Sigma Chemical Company, St. Louis,
MO). All drugs were dissolved in sterile water and administered subcutaneously by
osmotic pumps (cf. supra). F 13640, F 13714 and morphine were administered
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through one pump. The doses of F 13640 and morphine where the same as those
used in previous studies; these doses were the highest at which the compounds were
water-soluble, chemically stable, and sufficiently tolerated (Bruins Slot et al., 2002;
Colpaert et al., 2002); the 0.63 mg/day dose is also that at which F 13640 produced
full antinociceptive effects in the arthritic rat model (Colpaert et al., 2002); the dose of
F 13714 was four times smaller than that of F 13640, in accordance with its four to
sixteen times greater potency on bolus injection (Colpaert et al., 2002; Deseure et al.,
2002). Because of limitations in solubility, baclofen was administered through two
pumps, and rats treated with baclofen were compared with rats implanted with two
saline pumps. The baclofen dose corresponds to the largest concentration at which
baclofen was soluble and remained stable at 37° Celsius over two weeks
(unpublished observations). Sham operated rats were implanted with one saline
pump and compared to IoN-CCI rats that were also implanted with one saline pump.
Doses refer to the free base.
Drug effects in normal rats
All treatment conditions that were studied in the IoN-CCI model, were beforehand for
their tolerability in normal rats. Housing conditions were as described elsewhere
(Bruins Slot et al., 2002). Rats were implanted on Day 0 either with one pump
releasing 0.63 mg/day of F 13640, 0.16 mg/day of F 13714, 5 mg/day of morphine, or
saline, or with two pumps releasing 1.06 mg/day of baclofen or saline; each drug
treatment was studied in parallel with its own (saline) control group (n=7 / group).
Measurements were made of rectal body temperature (to the nearest 0.1°C) by
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means of a thermal probe (Ellat model RM6, Carrieri Instruments, Paris, France) and
of body weight (to the nearest gram). Measurements were made immediately before
pump implantation as well as 30 min, 60 min, 2h, 4h and 8h after pump implantation.
Further measurements were taken once daily (at 9:00 a.m.) on post-implantation
days 1, 2, 3, 4 and 7. At all of these times, an observation was also made of the rat’s
behavior in order to assess any behavioral anomaly.
Statistical Analysis
The response scores to mechanical stimulation that were obtained in the present
studies constitute an ordinal variable; accordingly, the data should be analyzed non-
parametrically and represented graphically by non-parametric measures of central
tendency and of variation. However, here as in a previous study (Deseure et al.
2002), non-parametric representation of the data using medians reflected the
statistical outcome of the non-parametric analyses quite poorly. The use of median
scores failed to appropriately visualize the nonetheless highly significant effects which
both the IoN injury and the agents produced. This mismatch results from the limited
variation which the scoring system allows.
Therefore, a parametric data analysis was done of which the statistical
outcome demonstrated an excellent concordance with that of the non-parametric
analysis. Of the in all 64 comparisons which the present studies called for, only 6
provided significance (p < .05) in the parametric analysis while not doing so in the
non-parametric analysis (non-parametric p-values were: .05, .07, .11, .11, .16 and
.18); and in one instance, the non-parametric analysis reached significance (i.e., p =
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.027) where the parametric analysis did not. Furthermore, the graphical
representation of the data by means of the average and standard error of the mean
(SEM) provided a far more satisfactory reflection of both analyses. Thus, here as
elsewhere (Deseure et al., 2002), data are both analyzed and represented in a
parametric manner.
For each rat and at every designated time, the mean of the response scores to
the five von Frey hairs was determined. Post-operative changes of base-line values
before drug treatment were analyzed by means of repeated measures ANOVA with
day as within subjects factor and followed by an unpaired t-test per time point. This
ANOVA and those described hereafter were performed separately on data obtained
after ipsilateral and after contralateral stimulation. Mechanical stimulation data
obtained during drug treatment were also analyzed by means of repeated measures
ANOVA with day as within subjects factor and followed by a unifactorial ANOVA per
time point. Post-hoc comparisons were carried out using Dunnett’s test for comparing
means with a control mean. Mechanical stimulation data obtained six days after drug
treatment were analyzed by means of a unifactorial ANOVA followed by post-hoc
comparisons carried out using Dunnett’s test for comparing means with a control
mean. Data obtained during and after drug treatments were analyzed separately for
rats implanted with one pump and rats implanted with two pumps.
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Results
EFFECTS EARLY AFTER SURGERY WHEN ALLODYNIA DEVELOPS
Responsiveness to ipsilateral mechanical stimulation in IoN-CCI rats was significantly
different from that in sham operated rats [groups * time interaction: F(5,110) = 37.45,
p<0.001] (Fig. 1). Post-hoc comparisons (unpaired t-tests) showed significant
differences on days 4, 8, 11, 13 and 15 (p<0.05), but not on day 6 (p=0.34).
Responsiveness to contralateral mechanical stimulation was not significantly different
between these groups [groups * time interaction: F(5,110) = 2.27, p>0.05]. Also, no
significant differences were found between IoN-CCI rats treated with one or two saline
pumps in responsiveness to ipsilateral [not shown; groups * time interaction: F(5,110)
= 1.56, p>0.05] or contralateral [not shown; groups * time interaction: F(5,110) =
1.04, p>0.05] stimulation.
Mechanical stimulation data shown in panels A, B and C of Fig. 2 were
obtained in rats implanted with one pump, and were analyzed together. The effects of
the drug treatments varied with time in a manner that was significantly different
among the drugs [treatments * time interaction: F(15,220) = 3.66, p<0.001]. Because
the effects of surgery changed over time (i.e., from an initial hyporesponsiveness,
through an apparent lack of effect, to hyperresponsiveness; see Fig. 1) possible drug
effects were analyzed at each of the time points. Unifactorial ANOVA per time point,
with treatments as between-subjects factor, showed significant differences between
the treatments on days 11, 13 and 15 [F(3,44) � 4.35, p�0.009], but not on days 4, 6
and 8 [F(3,44) ��������S�����@. As indicated with asterisks in the respective panels in
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Fig. 2, post-hoc comparisons between the different treatments and their respective
vehicle showed significant drug effects of F 13714 on days 13 and 15; F 13640 was
effective on days 11, 13 and 15; no significant drug effects were found for morphine.
The effects of F 13640 and F 13714 significantly changed over time [treatment * time
interaction: F (5,110) � 5.73, p<0.001]; in contrast, the effects of morphine, if any, did
not significantly change over time [treatment * time interaction: F(5,110) = 1.29,
p>0.05].
Mechanical stimulation data shown in panel D of Fig. 2 were obtained in rats
that were implanted with two osmotic pumps. No significant overall difference was
found between baclofen and saline treated rats, although the p-value fell only
narrowly short of significance [F(1,22) = 4.15, p=0.053]. Because the effect of the
surgery changed over time (see above), further analyses were conducted to detect
potential differences between baclofen and saline on one or more of the individual
time points. Post-hoc comparisons between baclofen and saline treated rats showed
significant differences on days 11, 13 and 15 (Fig. 2D). Despite this apparent
increase in the effects of baclofen, repeated measures ANOVA did not yield a
significant change in the effects of baclofen over time [treatment * time interaction:
F(5,110) = 0.92, p>0.05].
Since no significant differences were found between IoN-CCI rats and sham
operated rats for responsiveness to contralateral mechanical stimulation, no analysis
of possible drug effects was performed for this variable.
In an earlier study (Deseure et al., 2002), each of the compounds examined
here induced behavioral effects upon acute intraperitoneal injection in IoN-CCI
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lesioned rats. Signs of the so-called 5-HT syndrome were not observed in the course
of von Frey testing (i.e., starting three days after pump implantation) in any of the rats
treated with F 13640 or F 13714. Also, no signs of immobility or akinesia were
observed in rats treated with baclofen or morphine, respectively.
EFFECTS ON ALLODYNIA SIX DAYS AFTER REMOVAL OF OSMOTIC PUMPS
Unifactorial ANOVA on day 21, i.e., six days after the removal of the osmotic pumps,
showed significant differences between drug treatments for rats treated with one
pump [F(3,35) = 6.23, p<0.01]; no significant difference was found between rats
treated with two pumps [F(1,18) = 1.77, p=0.20] (Fig. 3). Post-hoc comparisons
showed that in rats that had been treated with either F 13640 or F 13714 during the
first two weeks following IoN-CCI surgery, hyperresponsiveness to mechanical
stimulation was still significantly reduced on day 21.
EFFECTS LATE AFTER SURGERY WHEN ALLODYNIA IS ESTABLISHED
Following IoN ligation, rats demonstrated significant changes in responsiveness to
mechanical stimulation of the territory of the ligated ipsilateral nerve [F(5,310) =
142.34, p<0.001] (Fig. 4). An initial decrease in ipsilateral response scores on day +6
(p<0.001), was followed by a marked increase on days +11 to +24 (p<0.001). The
rats also demonstrated a small but significant increase in responsiveness to
mechanical stimulation of the territory of the non-ligated contralateral IoN [F(5,310) =
39.92, p<0.001]. Post-hoc comparisons showed significant differences from day +6 to
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day +24 (p<0.001).
Mechanical stimulation data shown in panels A, B and C of Fig. 5 were
obtained in rats implanted with one pump, and were analyzed together. Unifactorial
ANOVA per time point, with treatments as between subjects factor, showed
significant differences between the treatments on all six time points [F(3,46) � 5.06,
p<0.01]. The effects of the drug treatments varied with time in a manner that was
significantly different among the drugs [treatments * time interaction: F(15,230) =
5.76, p<0.001]. Post-hoc comparisons between F 13640 and saline showed that F
13640 significantly reduced the hyperresponsiveness to mechanical stimulation at all
time points; its effects did not significantly change over time [treatment * time
interaction: F(5,120) = 0.61, p>0.05]. Significant effects were found for F 13714 on
days 34, 36 and 38; morphine was effective on all days except day 38. The ability of F
13714 to reduce the hyperresponsive behavior significantly increased over time
[treatment * time interaction: F(5,120) = 4.57, p<0.001]; in contrast, the effects of
morphine significantly decreased over time [treatment * time interaction: F(5,120) =
5.67, p<0.001].
Mechanical stimulation data shown in panel D of Fig. 5 were obtained in rats
that were implanted with two pumps. A significant overall difference was found
between baclofen and saline treated rats [F(1,16) = 5.17, p<0.05]; the effects of
baclofen treatment did not significantly interact with time [treatment * time interaction:
F(5,80) = 1.07, p>0.05]. Post-hoc comparisons between baclofen and saline treated
rats showed significant differences on days 29 through 38.
No significant differences were found for responsiveness to contralateral
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mechanical stimulation [not shown; F(15,230) � 1.52, p�����@� Signs of the 5-HT
syndrome were again not observed in the course of von Frey testing (i.e., starting
three days after pump implantation) in any of the rats treated with F 13640 or F
13714. Also, no signs of immobility or akinesia were observed in rats treated with
baclofen or morphine, respectively.
DRUG EFFECTS IN NORMAL RATS
None of the drug treatments exerted a significant effect on body weight (not shown).
All treatments, however, lowered body temperature (Fig. 6). Repeated measures
ANOVA of the F 13640 data indicated a significant effect of treatment [F(1,12) =
30.07, p<0.001], of time [F(10,120) = 12.35, p<0.001] and of their interaction
[F(10,120) = 25.80, p<0.001]. F 13640 ‘s hypothermic effect was apparent for up to 2
hours after pump implantation (Fig. 6). F 13714 produced a similar effect for up to 4
hours (F values: 19.21, 5.32 and 8.02 for the respective effects; p<0.001 in each
case). With morphine, no significant effect of treatment [F(1,12) = 0.00, p>0.05] was
found, but the effect of time [F(10,120) = 9.28, p<0.001] and that of the treatment *
time interaction [F(10,120) = 2.68, p<0.01] were significant; morphine’s hypothermic
effect was apparent 30 min, but not at any later time interval (Fig. 6). With baclofen,
the effects of treatment [F(1,12) = 11.64, p<0.01] and of time [F(10,120) = 10.73,
p<0.001] were significant, but not that of their interaction [F(10,120) = 0.77, p>0.05];
baclofen’s hypothermic effect was apparent for up to 60 min after pump implantation
(Fig. 6).
F 13640 induced lower lip retraction, flat body posture and forepaw treading for
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a few hours, and at most one day, after pump implantation; similar signs of the 5-HT
syndrome were observed with F 13714 (Fig. 6). No grossly observable behavioral
anomalies were found with morphine or baclofen.
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Discussion
In IoN-CCI rats that were implanted immediately after the surgery with either one or
two subcutaneous osmotic pumps releasing saline, hyperresponsiveness to
mechanical, von Frey hair stimulation developed in a manner similar to that described
previously (Vos et al., 1994; Deseure et al., 2002); this suggests that the pump
implantation did not noticeably interfere with the development of allodynia. That is,
the injury initially caused an almost complete loss of ipsilateral responsiveness that
was followed, from day 8 onward, by hyperresponsiveness (Fig. 1). The 5-HT1A
receptor agonist, F 13640, significantly reduced the hyperresponsiveness at the time
that the latter developed (Fig. 2); this was also found with the other 5-HT1A receptor
agonist, F 13714, though its effects required two more days to achieve statistical
significance. The effects of both agents are consistent with the duration-dependent
co-operation that is expected (Colpaert, 1996; Colpaert et al., 2002) to develop
between 5-HT1A receptor activation and nociceptive stimulation; the anti-allodynic
effect with either agent became more robust as the duration of co-exposure to both 5-
HT1A receptor activation and IoN-CCI - induced nociception grew longer. This
increase in effectiveness is not likely due to drug accumulation; circulating plasma
levels of F 13640 in rats similarly implanted with a pump releasing 0.63 mg/day,
reach asymptote some 24 hours after pump implantation, and then remain stable (at
about 80 ng/ml) throughout the further two-week period (unpublished observations).
Baclofen exerted a significant anti-allodynic effect from day 11 onward (Fig. 2).
Finally, response scores with morphine also were lower than with saline, but this
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difference was not significant. Morphine’s inability to produce any significant effect
here may be surprising in that acutely injected morphine in this model does produce a
significant anti-allodynic effect (DeMulder et al, 1994; Deseure et al., 2002). However,
in normal rats implanted with a morphine pump similarly releasing 5 mg/day,
tolerance to morphine’s analgesic action in the Randall-Selitto assay (Randall and
Selitto, 1957) developed within 24 hours after pump implantation (Colpaert et al.,
2002). Here, the first post-pump-implantation recordings of allodynic behavior were
made four days after morphine infusion was initiated. It is therefore possible that the
morphine pump implantation induced an anti-allodynic effect, but that tolerance had
developed by the time that the first recordings were made and hyperresponsiveness
was established.
When pumps were implanted at a time that allodynia had been well
established (i.e., 24 days after the IoN-CCI injury; Fig. 4), F 13640 caused an anti-
allodynic effect that now was statistically significant at all post-implantation times (Fig.
5); the effects of F 13714 required a longer duration of exposure for them to reach
significance. This difference between the two 5-HT1A ligands is consistent with the
expected (Colpaert, 1996; Colpaert et al., 2002) intensity-dependence of co-
operation; F 13640 exerts a greater activation of 5-HT1A receptors than F 13714
(Colpaert et al., 2002), so that its co-operation with nociception in inducing analgesia
should develop more effectively. In stark contrast with its apparent ineffectiveness
early after the injury (Fig. 2) as well as in other models of neuropathic pain (Colpaert
et al., 2002), morphine was clearly effective in the IoN-CCI model at this later stage
(Fig. 5). However, tolerance to morphine developed, and the compound no longer
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produced a significant effect two weeks after its continuous infusion had been
initiated. At the later stage, baclofen also was effective (Fig. 5).
Throughout the first week after their infusion had been initiated, neither F
13640 nor morphine produced any reliable effect in the first study (Fig. 2), while at
that same time both agents produced a significant and consistent anti-allodynic effect
in the second study (Fig. 5). These apparently larger analgesic effects of both F
13640 and morphine when allodynia was well established relative to those observed
early after the IoN-CCI injury, suggest that the ongoing nociceptive stimulation was of
higher intensity at that later stage. This is because the (initial) antinociceptive action
of both 5-HT1A and opioid receptor activation, must on theoretical grounds be
expected (Colpaert, 1996) to be larger as ongoing nociception is more intense (see
also: Colpaert, 1978, 1979; Colpaert et al., 1980).
Six days after pump removal, and not surprisingly, the analgesic effects, if any,
of both morphine and baclofen had disappeared (Fig. 3). Remarkably, however, the
effects of both F 13640 and F 13714 persisted at this point of time. The persistence
of this effect is not likely to be due to the prolonged presence of F 13640; while the
two-week infusion of 0.63 mg/kg generates a stable plasma level of about 80 ng/ml,
the latter drops below the 0.1 ng/ml detection limit three days after pump explantation
(unpublished observations). This may suggest that mechanical allodynia did not
develop to the same level in 5-HT1A treated rats compared with saline treated rats.
The development of neuropathic pain is considered to be due to neuronal
hyperexcitability, followed by neuroplastic changes and reorganization in the nervous
system (i.e., from a process of central sensitization: for review, see: Attal and
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Bouhassira, 1999; Ji and Woolf, 2001; Zimmerman, 2001). It is possible that the
intensity-dependent co-operation between 5-HT1A receptor activation and nociceptive
stimulation paradoxically attenuated this process of central sensitization. On the other
hand, as pointed out by Jensen (Jensen, 2002), the development of neuropathic pain
is preceded by a loss of sensory function, of nociceptive input in particular. This loss
is exemplified in the IoN-CCI model by the loss of ipsilateral responsiveness to
mechanical stimulation early after the injury (Figs. 1 and 4; see also: Deseure et al.,
2002). According to the concept that guided the present research, F 13640 mimics
the central effects of nociceptive input (Colpaert, 1996; Colpaert et al., 2002). It is
therefore possible that F 13640 pre-empts the development of neuropathic allodynia
in the IoN-CCI model by providing the input that lacks early after the somatosensory
nerve injury.
Transient hypothermia and signs of the 5-HT syndrome were observed during
the first hours after pump implantation in rats treated with F 13640 and F 13714 (Fig.
6). In view of the neuroprotective properties of hypothermia (Marasal et al., 1994;
Gunn and Gunn, 1998) and of 5-HT1A receptor agonists (Uchiyama et al., 2001), it is
conceivable that the anti-allodynic effects of F 13640 and F 13714, six days after
pump removal, to some extent reflect a reduced development of allodynia caused by
the neuroprotection during the first hours after IoN-CCI surgery. However, for up to
eight days after pump implantation, response scores in F 13640 and F 13714 treated
rats were similar to those in rats treated with morphine or baclofen (Fig. 2), in spite of
the latter two agents producing a much smaller and shorter-lived hypothermia (Fig. 6).
Furthermore, at the time that allodynia started to develop, i.e. from day 4 to day 6
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onward (Fig. 1), none of the agents any longer produced hypothermia. It is also
unclear how these putative neuroprotective properties could account for the anti-
allodynic effects that were observed when treatment was started only at a time when
allodynia was already well established. Still, it would be interesting to investigate
whether experimentally controlled hypothermia during or immediately following IoN
ligation, can prevent or attenuate the development of mechanical allodynia in this
model.
The present data may also contribute to resolve the much debated controversy
concerning the ability of opioids to relieve neuropathic pain (Arnér and Meyerson,
1988; Portenoy et al., 1990; Xu et al., 1999). The acute injection of high morphine
doses in particular induces an anti-allodynic effect in the IoN-CCI model (DeMulder et
al., 1994; Deseure et al., 2002) as well as in other animal models of neuropathic pain
(Attal et al., 1991; Ossipov et al., 1999; Bulka et al., 2002). However, as morphine is
administered chronically, both the magnitude of its (initial) analgesic action and the
rate at which tolerance develops, depend on persistent, ongoing nociception
(Colpaert, 1978, 1979, 1996; Colpaert et al., 1980). This explains why morphine,
administered at a time when allodynic hyperresponsiveness was not or not fully
established (Fig. 1), produced little, if any, anti-allodynic effect (Fig. 2), presumably
because, during the hypo-responsive phase, tolerance had developed; and why
morphine, administered only at a time when allodynia was fully established, produced
an initially powerful anti-allodynic effect to which a tolerance developed that required
two weeks for it to result in a complete loss of its effectiveness (Fig. 5). In
comparison, and as mentioned above, in normal rats that are not exposed to any
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persistent nociception, complete tolerance develops to the same 5 mg/day dose of
morphine within 24 hours (Colpaert et al., 2002). Similar to the present data, Bulka et
al. (2002) found that in mononeuropathic rats injected (s.c.) twice daily with 10 mg/kg
of morphine, mechanical hypersensitivity to von Frey hair stimulation of the hind paw
was initially inhibited, but after two weeks morphine’s effects had disappeared almost
entirely. Thus, while the acute administration of high morphine doses in particular
may effectively alleviate neurogenic allodynia and induce side effects that are
characteristic of opioids (e.g., Deseure et al., 2002), during chronic administration,
tolerance to opioids develops at a rate that depends on the magnitude of the
persistent nociception that is associated with the neuronal injury. This predictably
results in observations that morphine’s ability to alleviate neuropathic allodynia is
transient and of a variable duration.
It is useful to note that, at the doses used here, neither F 13640 nor F 13714
fully restored mechanical responsiveness to the pre-injury level (Fig. 5). It is likely,
however, that higher doses would produce a full effect; both these agents, as well as
morphine and baclofen, produce a full anti-allodynic effect in this model upon their
acute injection at sufficiently high doses (at a post-injury time at which allodynia was
well established; Deseure et al., 2002). Indeed, the issue addressed here concerned
the neuroadaptive changes to these effects in a condition where the compounds are
administered chronically. In this condition, the effect of morphine decayed, that of
baclofen remained stable, while that of the 5-HT1A receptor agonists, if anything,
increased (Fig. 5).
In conclusion, the present findings indicate that the continuous infusion of the
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high-efficacy 5-HT1A receptor agonist, F 13640, produces definite anti-allodynic
effects in the IoN-CCI rat model of trigeminal neuropathic pain. Consistent with a
concept of signal transduction in nociceptive systems, 5-HT1A receptor activation
appeared to co-operate, in a both duration- and intensity-dependent manner, with
persistent nociception in this model, to produce anti-allodynic effects. In contrast to
the tolerance that developed to morphine-induced anti-allodynic effects, the effects
produced by F 13640 did not decay. Equally, and in contrast to both morphine and
baclofen, the anti-allodynic effects of F 13640 persisted six days after its
administration had been discontinued. Further investigations of F 13640’s
mechanisms and usefulness in the treatment of trigeminal neuropathic pain should
address several issues. Inverse tolerance developed to some extent to F 13640’s
anti-allodynic action over the two-week period of continuous infusion; it would be of
interest to determine whether the anti-allodynic effect would continue to grow with
longer infusion periods. The molecular and neuroadaptive mechanisms of these
agents being very different, it would also be of interest to examine the effects of
chronically administered F 13640 in association with baclofen and morphine. Finally,
the persistent anti-allodynic effect that F 13640 produced six days after its
administration was discontinued, warrants further study; this possible pre-emptive
action on the development of neuropathic allodynia is scientifically and potentially
also clinically interesting.
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Acknowledgements
The authors thank K. Szewczyk for assistance in conducting the experiments.
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Legends to Figures
Figure 1. Time course of the effects of IoN-CCI ligation on responsiveness to
mechanical stimulation. Data points represent the mean (± SEM) response score to
von Frey hair stimulation of the territory of the ligated nerve (closed symbols) and of
the contralateral side (open symbols) of IoN-CCI rats (n=12; triangles) and sham
operated rats (n=12; circles) one day before (Pre) and 4 to 15 days after surgery.
Asterisks indicate a significant difference compared to sham operated control rats
(unpaired t-test, * p<0.05, *** p<0.001).
Figure 2. Effects of F 13640, F 13714, morphine, and baclofen on
responsiveness to mechanical stimulation early after IoN-CCI ligation when
allodynia develops. Data points represent the mean (± SEM) response score to von
Frey hair stimulation of the territory of the ipsilateral, ligated nerve of rats treated with
F 13640 (0.63 mg/day; n=12; A), F 13714 (0.16 mg/day; n=12; B), morphine (5
mg/day; n=12; C), baclofen (1.06 mg/day; n=12; D) or their respective vehicle (n=12)
from day 0 to day 15 following IoN-CCI ligation. Mechanical stimulation data in panels
A, B and C were obtained in rats implanted with one pump; data shown in panel D
were obtained in rats implanted with two pumps. Drug or vehicle infusion via osmotic
pumps started immediately after IoN-CCI ligation on day 0. Asterisks indicate a
significant difference compared to vehicle control rats (Dunnett’s test, * p<0.05, **
p<0.01, *** p<0.001).
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Figure 3. Effects of F 13640, F 13714, morphine, and baclofen on
hyperresponsiveness to mechanical stimulation six days after removal of the
osmotic pumps releasing these agents. Data points represent the mean (+ SEM)
response score to von Frey hair stimulation of the territory of the ipsilateral, ligated
nerve of rats that had been infused with F 13714 (n=12), F 13640 (n=12), morphine
(n=12), baclofen (n=12), or their respective vehicle (n=12) during the first two weeks
following IoN-CCI ligation (see also legend to Fig. 2). Mechanical stimulation data
shown on the left were obtained in rats implanted with one pump; data shown on the
right were obtained in rats implanted with two pumps. Drug or vehicle infusion via
osmotic pumps started immediately after IoN-CCI ligation on day 0 and was
terminated on day 15 when the pumps were removed. Rats were subsequently tested
on day 21. Asterisks indicate a significant difference compared to vehicle control rats
(Dunnett’s test, * p<0.05, *** p<0.001).
Figure 4. Time course of the effects of IoN-CCI ligation on responsiveness to
mechanical stimulation. Data points represent the mean (± SEM) response score to
von Frey hair stimulation of the territory of the ligated nerve (ipsilateral) and of the
contralateral side (contralateral) one day before (Pre) and 6 to 24 days after surgery
(n=68; these animals were implanted with pumps immediately after mechanical
stimulation testing on day 24, see Fig. 5). Asterisks indicate a significant difference
compared to the respective pre-operative values (Dunnett’s test, *** p<0.001). Note
the similarity of these data to those presented in Fig. 1.
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Figure 5. Effects of F 13640, F 13714, morphine, and baclofen on hyper-
responsiveness to mechanical stimulation late after IoN-CCI ligation when
allodynia is well established. Data points represent the mean (± SEM) response
score to von Frey hair stimulation of the territory of the ipsilateral, ligated nerve of rats
that were infused with F 13640 (0.63 mg/day; n=12; A), F 13714 (0.16 mg/day; n=12;
B), morphine (5 mg/day; n=12; C), baclofen (1.06 mg/day; n=9; D) or their respective
vehicle (n=14 for A-C; n=9 for D) from day 24 to day 38 following IoN-CCI ligation.
Mechanical stimulation data in panels A, B and C were obtained in rats implanted
with one pump; data shown in panel D were obtained in rats implanted with two
pumps. Drug or vehicle infusion via osmotic pumps started immediately after
mechanical stimulation testing on day 24. Asterisks indicate a significant difference
compared to vehicle control rats (Dunnett’s test, * p < 0.05, ** p < 0.01, *** p < 0.001).
Figure 6. Drug effects in normal rats. Data points in panel A represent the mean (±
SEM) rectal temperature immediately before and at the indicated hours and days
after normal rats were implanted with pumps releasing F 13640 (0.63 mg/day), F
13714 (0.16 mg/day), morphine (5 mg/day), baclofen (1.06 mg/day) or saline (n=7 per
group). Asterisks indicate a significant difference compared to control animals
(Dunnett’s test, * p < 0.05, ** p < 0.01, *** p < 0.001). Data points in panels B, C and
D represent the number of rats (out of n=7) demonstrating lower lip retraction, flat
body posture or forepaw treading, respectively.
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0
1
2
3
4
Pre 4 6 8 11 13 15
Time (days post-operative)
Res
pons
e S
core
(Mea
n ±
SE
M)
IoN-CCI ipsilateralsham ipsilateral
IoN-CCI contralateralsham contralateral
*** *** ***
*
***
Figure 1
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0
1
2
3
4
Pre 0 4 6 8 11 13 15
saline
baclofen
Start ofdrug
treatment
*****
0
1
2
3
4
Pre 0 4 6 8 11 13 15
Res
pons
e S
core
(Mea
n ±
SE
M)
saline
morphine
Start ofdrug
treatment
0
1
2
3
4
Pre 0 4 6 8 11 13 15
saline
F 13714
Start ofdrug
treatment
** **
0
1
2
3
4
Pre 0 4 6 8 11 13 15
Res
pons
e S
core
(Mea
n ±
SE
M)
saline
F 13640
Start ofdrug
treatment
*****
A.
C.
B.
D.
Time (days post-operative) Time (days post-operative)
***
Figure 2
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0
1
2
3
4
F 13640 F 13714 Morphine Saline Baclofen Saline
Drug treatments
Res
po
nse
Sco
re(M
ean
+ S
EM
) *** *
Figure 3
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0
1
2
3
4
Pre 6 11 16 21 24
Time (days post-operative)
Res
pons
e S
core
(M
ean
± S
EM
)
ipsilateral
contralateral
***
***
***
***
***
***
***
***
***
***
Figure 4
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0
0.5
1
1.5
2
2.5
3
3.5
4
24 27 29 31 34 36 38
Time (days post-operative)
saline
baclofen
Start of drug
treatment
* *
** *
0
0.5
1
1.5
2
2.5
3
3.5
4
24 27 29 31 34 36 38
Time (days post-operative)
Res
pons
e S
core
(M
ean
± S
EM
)
saline
morphine
Start of drug
treatment
******
***
*
0
0.5
1
1.5
2
2.5
3
3.5
4
24 27 29 31 34 36 38
saline
F 13714
Start of drug
treatment
** ** **
0
0.5
1
1.5
2
2.5
3
3.5
4
24 27 29 31 34 36 38
Res
pons
e S
core
(Mea
n ±
SE
M)
saline
F 13640
Start of drug
treatment
* *
*** **
A. B.
C. D.
Time (days post-operative) Time (days post-operative)
**
**
*
Figure 5
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313233343536373839
0 ½ 1 2 4 8 1 2 3 4 7
°C
salineF13640
*** ***
***
313233343536373839
0 ½ 1 2 4 8 1 2 3 4 7
°C
salineF 13714 ***
*** ***
*
A. Body temperature
°C
°C
313233343536373839
0 ½ 1 2 4 8 1 2 3 4 7
°C
salinebaclofen
** **
°C
313233343536373839
0 ½ 1 2 4 8 1 2 3 4 7
hours days
°C
salinemorphine
**°C
0
1
2
3
4
5
6
7
0 ½ 1 2 4 8 1 2 3 4 7
hours days
saline
F 13714
0
1
2
3
4
5
6
7
0 ½ 1 2 4 8 1 2 3 4 7
saline
F 13714
0
1
2
3
4
5
6
7
0 ½ 1 2 4 8 1 2 3 4 7
saline
F 13714
0
1
2
3
4
5
6
7
0 ½ 1 2 4 8 1 2 3 4 7
hours days
saline
F13640
0
1
2
3
4
5
6
7
0 ½ 1 2 4 8 1 2 3 4 7
saline
F13640
0
1
2
3
4
5
6
7
0 ½ 1 2 4 8 1 2 3 4 7
saline
F13640
B. Lower lip retraction
D. Forepaw treading
C. Flat body posture
n
n
n
Figure 6
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