1. CFDA/CK19 Immunoflourescence
P58 DKO n=2
P70 HNF-6 KO n=1
P70 NICD/HNF-6 n=3

2. DKO P58: 1639 P58 male, T bili 7.9, weight 25.80gms
CFDA
CK19
Hilar ducts typically with good CFDA co-stain
Multiple areas in periphery with disorganized CK19 positive cells not CFDA positive
Pictures intralobular to periphery
3. DKO P58: 1540 P58 male, T bili39.7, weight 13.11gms, very ill appearing
CFDA
CK19
Much fewer areas of disorganized CK19 cells
Some hilar ducts with poor CFDA stain
Overall, very limited CFDA
Hilar duct negative with small duct adjacent postive for CFDA
4. P70 HNF-6 KO
CFDA
CK19
Normal
All ducts with CFDA
No ducts without CFDA
5. P70 NICD/HNF-6: 1575
CFDA
CK19
Most well formed ducts positive for CFDA
Multiple areas along periphery and hilum without CFDA positive staining, mostly if associated with areas of disorganized CK19+ cells
6. P70 NICD/HNF-6: 1876
CFDA
CK19
Most well formed ducts positive for CFDA
Multiple areas along periphery and hilum without CFDA positive staining, mostly if associated with areas of disorganized CK19+ cells