cip bangladesh research reports on potato-2009-10

40
1 Annual Reports 2009-10 Compiled Compiled Compiled Compiled By By By By Md. Mohidul Hasan Dr. M. Hossain & Dr. M.S. Kadian Tuber Crops Research Centre (TCRC) Bangladesh agricultural research institute (BARI) Joydebpur, Gazipur-1701, Bangladesh Tel: +88 02 9252534 Fax: +88 02 9262908 International Potato Center (CIP), SWCA CIP Local Office TCRC, BARI, Joyebpur, Gazipur-1701 Bangladesh

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1

Annual Reports

2009-10

Compiled Compiled Compiled Compiled ByByByBy

Md. Mohidul Hasan

Dr. M. Hossain

&

Dr. M.S. Kadian

Tuber Crops Research Centre (TCRC) Bangladesh agricultural research institute (BARI)

Joydebpur, Gazipur-1701, Bangladesh

Tel: +88 02 9252534

Fax: +88 02 9262908

International Potato Center (CIP), SWCA

CIP Local Office

TCRC, BARI,

Joyebpur, Gazipur-1701

Bangladesh

2

CONTENTSCONTENTSCONTENTSCONTENTS

Expt. no. Title of the Experiment

Page no.

1 Secondary Yield Trial (SYT) with Late Blight Disease

Resistance CIP Potato Germplasm

1

2 Screening of Potato Varieties and CIP Germplasm Against

Heat Tolerance

7

3 Degeneration Study of Virus Resistance CIP Germplasm in

Bangladesh

12

4 Screening of Potato Varieties and CIP Germplasm

Against Salt Tolerance

18

5 Screening of CIP Clones Against Drought Tolerance

22

3

EXECUTIVE SUMMARY

Seven CIP clones viz., LB-3, LB-4, LB-6, LB-7, LB-8, LB-12 and LB-14 were evaluated against

late blight disease of potato under field condition at Bogra, Jamalpur and Munshiganj and variety

Diamant was used as check. Among the tested clones the highest average yield (38.59 t/ha) was

obtained in line LB-7 followed by LB-8. Considering late blight infection and the yield

contributing characters for 2007-08, 2008-09 and 2009-10, three clones LB-3, LB-6 and LB-7

were selected for advanced yield trial in next season.

Five CIP germplasms, like LB-1, LB-2, LB-7, LB-14 and LB-12 one TCRC improved potato

variety Diamant and one local variety Dohazari Sada were evaluated at Pahartoli, Chittagong for

their suitability against heat tolerance. Among all the studied varieties/germplasm, LB-7 was the

highest yielder (22.98 t/ha) followed by LB-1 & Diamant (20.81 t/ha).

Eleven CIP clones viz. CIP-1, CIP-2, CIP-3, CIP-4, CIP-5, CIP-6, CIP-8, CIP-9, CIP-10, CIP-13

and CIP-14 with as check variety Diamant were evaluated at Jamalpur and Jessore respectively

during 2009-10 for their suitability against virus resistant. At Jamalpur the highest tuber yield was

obtained from CIP-10 (51.97 t/ha) followed by CIP-8 with ware practice. At Jessore the highest

yield was obtained from CIP-9 (48.40 t/ha) with improved practice followed by CIP-2 (42.08 t/ha)

Seven CIP germplasm viz (LB-3, LB-4, LB-6, LB-7, LB-8, LB- 12, LB-14) one TCRC improved

potato variety Diamant and one local variety Dohazari Sada were evaluated at Ukhia, Cox’s Bazar,

for their suitability against salt tolerance. At 40 DAP the salinity in the soil was (8.9 ds/m) and all

the cones died.

In drought tolerance trial, Seven( LB-1, LB-2, LB-3, LB-7, LB-9, LB-12 and LB-14) and eleven

CIP-101, CIP-102, CIP-111, CIP-117, CIP-124, CIP-126, CIP-129, CIP-130, CIP-134, CIP-137

and CIP-139) CIP clones with as checked variety Lalpakri, Lalashil and Diamant were evaluated at

Bogra and Dinajpur respectively for their suitability against drought tolerance. Considering yield

and yield attributes, the CIP clones LB-7 produced 34.3 t/ha with normal irrigation on the other

hand 33.8 t/ha produced under one irrigation practiced at Bogra. At Dinajpur the highest yield

CIP-101 (47.62 t/ha) and (42.00 t/ha) was higher produced with normal irrigation (three irrigation)

and one irrigation respectively.

4

Experiment 1: Secondary Yield Trial (SYT) With Late Blight Disease

Resistance CIP Potato Germplasm

T.K. Dey, M. Hossain, B.K. Goswami, M.H. Reja, M.A. Haque, M.M. Rahaman, E.H.M.S. Rahaman,

M. M. Hasan and M.S. Kadian

Abstract

Seven CIP clones LB 3 (391058.175), LB 4 (393077.159), LB 6 (393280.64), LB 7 (393371.58),

LB 8 (395011.2), LB 12 (396031.119) and LB 14 (396244.12) were evaluated against late blight

disease of potato under field condition at Bogra, Jamalpur and Munshiganj and variety Diamant

was used as check. There was no late blight infection in any germplasms due to unfavorable

condition of disease development in current season. Among the tested clones the highest average

yield (38.59 t/ha) was obtained in line LB-7 followed by LB-8. Considering late blight infection

and the yield contributing characters for 2007-08, 2008-09 and 2009-10, three clones LB-3, LB-6

and LB-7 were selected for advanced yield trial in next season.

Introduction

Late blight caused by Phytophthora infestans is one of the most destructive diseases of

potato. Late blight is a serious problem for potato production in Bangladesh. The disease

causes serious yield loss depending upon the stages of plant growth, susceptibility of

variety, and aggressiveness of the pathogen and prevailing environmental factors (Dey et.

al., 1995, Hossain et al., 2002). Cultivation of resistant varieties/cultivars is the best option

against any plant disease. So, the present study was undertaken to find out resistant

source(s) if any in the newly introduced international Potato Centre (CIP) potato clones

against late blight disease.

Materials and Methods

Seven CIP clones were evaluated for late blight resistant at three locations namely Spices Research

Center, Bogra, RARS Jamalpur and TCRSC, Munshiganj. The CIP clones included in the late

blight trial were LB 3 (391058.175), LB 4 (393077.159), LB 6 (393280.64), LB 7 (393371.58), LB

8 (395011.2), LB 12 (396031.119) and LB 14 (396244.12) and the variety Diamant was used as

check. Plant spacing was 60 cm X 25 cm, Plot size 2.4 m X 3.0 m in RCB design with 3

replications. The date of planting was 24.11.2009 at Bogra and Jamalpur, and 22.11.2009 was at

Munshiganj. Fertilizers were applied @ 325-220-250-120 kg/ha of Urea, TSP, MP and Gypsum,

respectively. Full amount of TSP, MP and Gypsum and 50% of urea were applied as basal and the

remaining amount of urea was side dressed at 35 DAP. Weeding, irrigation, earthing-up and other

intercultural operations were done as and when necessary. No fungicide was used in the trial. Late

blight data were taken on per cent foliage infection visually under natural condition. The crop was

5

harvested at 90 DAP in all locations. The date of planting 17, 18, 20 February 2010 at Bogra,

Jamalpur and Munshiganj respectively. All the data yield and yield contributing characters were

recorded and was statistically analyzed MSTATC program. Plant vigor was recorded on 1-5 scale,

where 1 is very poor vigor and 5 is highly vigorous.

Results and Discussion

There was a significant difference of the CIP lines towards growth parameter and different yield

contributing characters of potato. The details of the results were presented in (Table 1.1 to 1.6). In

this year there are no infection of late blight in all CIP clones including check variety of Diamant

due to unfavorable environment condition for late blight development. The weather of this year in

Bangladesh was not favourable for late blight.

Number of plant per plot at 45 days after planting (DAP), vigor (1-5 scale), senescence, tuber

weight (kg), tuber number , marketable t/ha, yield t/ha, average tuber weight (g), dry matter

percent (DM%) and average tuber weight (g) varied significantly at 90 DAP at Bogra and

Jamalpur and Munshiganj (Table 1.1 & 1.2).

Table 1.1 Performance of CIP late blight resistant clones at three locations in Bangladesh

during 2009-10

A. Bogra

CIP/ TCRC

clones/variety

No of Plant

per plot at

45 DAP

Vigor

(1-5

scale)

Senescence Tuber weight per plot (kg)

Marketable Non-

Marketable

Total

LB 3 (391058.175) 30.67 ab 5.00 a 2.67 ab 10.38 b 0.36 a 10.75 bc

LB 4 (393077.159) 21.67 c 4.33 a 1.00 d 7.80 c 0.17 a 7.97 d

LB 6 (393280.64) 28.33 b 4.67 a 1.67 cd 10.20 b 0.19 a 10.39 c

LB 7 (393371.58) 31.00 ab 5.00 a 2.33 abc 12.92 a 0.19 a 13.11 a

LB 8 (395011.2) 30.33 ab 4.67 a 1.00 d 11.10 ab 0.25 a 11.35 abc

LB12(396031.119) 31.67 a 4.33 a 1.00 d 11.60 ab 0.30 a 11.90 abc

LB 14 (396244.12) 22.00 c 4.33 a 2.00 bc 7.80 c 0.10 a 7.90 d

Diamant 31.67 a 4.67 a 3.00 a 12.10 ab 0.16 a 12.26 ab

CV (%) 5.64 11.56 28.85 9.70 81.12 9.14

LSD0.05 2.71 0.90 0.89 1.71 0.30 1.65

6

B. Jamalpur

TCRC/CIP

clones/variety

No of

Plant

per plot

at 45

DAP

Vigor

(1-5

scale)

Senescence Tuber Weight per plot (kg)

Marketable Non-

Marketable

Total

LB3(391058.175) 32.00 a 5.00 a 3.00 a 20.60 ab 0.32 b 20.92 ab

LB(393077.159) 32.00 a 4.33 a 1.00 b 20.43 abc 0.25 bc 20.68 abc

LB 6 (393280.64) 32.00 a 4.00 a 1.00 b 16.67 c 0.25 bc 16.92 c

LB 7 (393371.58) 32.00 a 5.00 a 2.33 a 24.43 a 0.17 cd 24.60 a

LB 8 (395011.2) 32.00 a 4.33 b 1.00 b 21.57 abc 0.37 ab 21.94 abc

LB12(396031.119) 32.00 a 5.00 a 1.00 b 21.67 abc 0.47 a 22.11 abc

LB 14 (396244.12) 32.00 a 4.33 b 1.00 b 18.13 bc 0.08 d 18.22 bc

Diamant 32.00 a 4.00 b 3.00 a 19.77 abc 0.26 bc 20.03 abc

CV (%) 0.00 6.52 24.49 13.43 27.55 13.25

LSD0.05 0.00 0.50 0.69 4.68 0.12 4.68

C. Munsiganj

TCRC/CIP

clones/variety

No of

Plant per

plot at 45

DAP

Vigor

(1-5

scale)

Senescence Tuber weight per plot (kg)

Marketable Non-

Marketable

Total

LB 3 (391058.175) 30.00 abc 4.67 ab 2.67 ab 9.40 ab 2.57 b 11.97 cd

LB 4 (393077.159) 26.67 d 4.67 ab 1.00 e 11.07 a 2.80 b 13.87 bc

LB 6 (393280.64) 28.67 c 4.00 b 1.67 cde 8.03 b 2.10b 10.13 d

LB 7 (393371.58) 31.00 a 5.00 a 2.33 bc 11.23 a 5.67 a 16.90 a

LB 8 (395011.2) 31.00 a 4.67 ab 1.33 de 11.70 a 3.33 b 15.03 ab

LB12(396031.119) 29.00 bc 4.00 b 1.33 de 10.00 ab 2.87 b 12.88 bcd

LB 14 (396244.12) 30.33 ab 5.00 a 2.00 bcd 10.60 ab 3.00 b 13.60 bc

Diamant 30.67 a 4.67 ab 3.33 a 9.80 ab 2.73 b 12.53 bcd

CV (%) 3.00 8.91 26.13 14.99 29.94 11.22

LSD0.05 1.503 0.69 0.86 2.59 1.58 2.53

Plant vigor on 1 to 5 Scale; 1: Very poor vigor and 5: Highly vigorous

7

Table 1.3 Yield performance of CIP late blight resistant clones at three locations in

Bangladesh during 2009-10

A. Bogra

TCRC/

CIP clones/

Variety

Tuber Number per Plot Marketable

t/ha

Yield

t/ha

Avg.

tuber

weight

(g)

DM (%)

Marketable Non-

Marketable

Total

LB 3 165.33 c 29.00 abcd 194.33 c 22.57 b 23.37 b 55.41 cd 20.24 ab

LB 4 115.00 d 17.33 cd 132.33 d 23.88 ab 24.37 ab 59.98 bc 17.35 cd

LB 6 205.33 ab 42.00 abc 247.33 ab 23.98 ab 24.42 b 42.07 e 18.53 bc

LB 7 205.00 ab 31.67 abcd 236.67 b 27.78 a 28.18 a 55.37 cd 19.30 abc

LB 8 198.00 b 47.33 ab 245.33 b 24.51 ab 25.04 ab 46.55 de 19.33 abc

LB 12 231.67 a 49.33 a 281.00 a 24.44 ab 25.06 ab 42.48 e 20.87 a

LB 14 77.33 e 13.00 d 90.33 e 23.52 b 23.84 b 90.34 a 16.09 d

Diamant 160.00 c 23.33 bcd 183.33 c 25.48 ab 25.89 ab 67.45 b 19.44 ab

CV (%) 10.53 42.20 9.92 8.75 8.05 11.43 5.73

LSD0.05 30.18 22.54 33.75 3.62 3.40 11.10 1.83

B. Jamalpur

TCRC/

CIP

clones/

Variety

Tuber Number per Plot Marketable

t/ha

Yield

t/ha

Avg. tuber

weight (g)

DM (%)

Marketable Non-

Marketable

Total

LB 3 219.67 b 40.33 bc 260.00 abc 47.08 ab 47.75 ab 88.35 bc 20.67 a

LB 4 181.00 d 31.00cd 212.00 bcd 42.57 abc 43.08 abc 97.91 b 18.33 a

LB 6 161.33 e 38.67 c 200.00 cd 34.75 c 35.24 c 83.25 bc 21.00 a

LB 7 255.00 a 20.33 cd 275.33 ab 50.90 a 51.25 a 90.12 bc 20.33 a

LB 8 254.67 a 58.00 ab 312.67 a 44.93 abc 45.70 abc 70.87 c 20.33 a

LB 12 256.67 a 68.33 a 325.00 a 45.14 abc 46.07 abc 68.53 c 20.67 a

LB 14 138.67 f 11.33 d 150.00 d 37.78 bc 37.95 bc 123.85 a 19.00 a

Diamant 198.33 c 30.00 cd 228.33 bc 41.18 abc 41.52 abc 89.45 bc 19.67 a

CV (%) 14.92 29.26 14.68 13.43 13.23 15.50 7.04

LSD0.05 52.46 18.41 60.85 9.76 9.737 23.32 2.38

D. Munsiganj

TCRC/

CIP

clones/

Variety

Tuber Number per Plot Marketable

t/ha

Yield t/ha Avg.

tuber

weight

(g)

DM (%)

Marketable Non-

Marketable

Total

LB 3 145.67 ab 64.00 ab 209.67 abc 20.82 b 26.52 cd 57.17 bc 19.30 abc

LB 4 151.00 ab 39.67 b 190.67 bc 27.75 a 34.72 ab 74.32 a 17.93 bc

LB 6 144.67 ab 73.67 ab 218.33 abc 18.81 b 23.69 d 46.26 c 17.70 c

LB 7 168.67 ab 95.33 a 264.00 a 24.16 ab 36.34 a 64.91 ab 20.60 a

LB 8 183.00 ab 75.67 ab 258.67 a 25.16 ab 32.33 abc 58.19 bc 19.20 abc

LB 12 195.00 a 73.67 ab 268.67 a 23.02 ab 29.56 bcd 48.35 c 19.70 ab

LB 14 184.33 ab 67.67 ab 252.00 ab 23.35 ab 29.91 bcd 55.38 bc 19.03 abc

Diamant 140.00 b 42.33 b 182.33 c 21.31 ab 27.23 cd 69.06 ab 18.17 bc

CV (%) 17.31 32.80 14.74 15.58 11.13 14.44 5.17

LSD0.05 47.96 36.84 57.39 6.07 5.65 14.44 1.66

8

Yield is the ultimate goal of any crop. In this trial, significant variation was observed at three

locations LB 7 (393371.58) average yield (38.59 t/ha) was the highest yield at 90 DAP harvesting.

And the lowest was recorded in LB 6 (393280.64) average yield (27.78 t/ha) where as Diamant

average yield (31.55 t/ha), the second highest LB-8 (34.36 t/ha), LB-4 (34.04 t/ha), LB-12 (33.56

t/ha) and LB-3 (32.55 t/ha) respectively on an average of three locations gave considerably higher

yield while check variety Diamant yielded the third lowest (31.55 t/ha) among all the clones (Table

1.4 & 1.5 ).

Table 1.4 Yield performance of CIP late blight resistant clones at three locations in

Bangladesh during 2009-10

TCRC/CIP clones/variety

Average tuber wt. (g) 90 DAP

Bogra Jamalpur Munshiganj Mean

LB 3 (391058.175) 55.41 88.35 57.17 66.98

LB 4 (393077.159) 59.98 97.91 74.32 77.40

LB 6 (393280.64) 42.07 83.25 46.26 57.19

LB 7 (393371.58) 55.37 90.12 64.91 70.13

LB 8 (395011.2) 46.55 70.87 58.19 58.54

LB 12 (396031.119) 42.48 68.53 48.35 53.12

LB 14 (396244.12) 90.34 123.85 55.38 89.86

Diamant 67.45 89.45 69.06 75.32

CV (%) 11.43 15.50 14.44 -

LSD0.05 11.10 23.32 14.44 -

Table 1.5 Yield performance of CIP late blight resistant clones at three locations in

Bangladesh during 2009-10

TCRC/CIP clones/variety

Tuber yield (t/ha) at 90 DAP

Bogra Jamalpur Munshiganj Mean

LB 3 (391058.175) 23.37 47.75 26.52 32.55

LB 4 (393077.159) 24.37 43.08 34.72 34.06

LB 6 (393280.64) 24.42 35.24 23.69 27.78

LB 7 (393371.58) 28.18 51.25 36.34 38.59

LB 8 (395011.2) 25.04 45.70 32.33 34.36

LB 12 (396031.119) 25.06 46.07 29.56 33.56

LB 14 (396244.12) 23.84 37.95 29.91 30.57

Diamant 25.89 41.52 27.23 31.55

CV (%) 8.05 13.23 11.13

LSD0.05 3.40 9.737 5.65

9

Table 1.6 Participatory yield trial of CIP late blight resistant clones at two farmer’s field in

Bangladesh during 2009-10

Name of the

clones/variety

Yield t/ha

Bogra Munshiganj Jamalpur Mean

LB 3 (391058.175) 19.07 30.0 26.33 25.13

LB 4 (393077.159) 24.31 25.5 38.89 29.57

LB 6 (393280.64) 26.32 15.6 26.67 22.86

LB 7 (393371.58) 35.45 33.3 43.33 37.36

LB 8 (395011.2) 18.10 30.0 30.14 26.08

LB 12 (396031.119) 27.91 - 33.06 30.37

LB 14 (396244.12) 23.67 35.5 34.58 31.25

Diamant 22.61 32.2 25.42 26.74

Remarks & farmers reaction: At farmers yield trial LB-7 was the highest yielder and they also like red skin uniform shaped LB-

12.

Conclusion: Considering the above factors shown in the above table revealed that clones CIP LB-7 produced

the highest yield followed LB-4 and LB-8. Moreover, skin color, flesh color and shape of the

potatoes were good and remarkable in LB-6 and LB-7. So these materials can be tested for

advanced yield trial.

References

Dey, T.K., Ali, M.S. and M.A. Hoque. 1995. Efficacy of fungicides in controlling late blight of potato.

Bangladesh Journal of Agricultural Research, 20(1): 62-66.

Hossain M., Dey T.K., Haque M.A., Rahman M.M. and M.S. Ali. 2002. Studies on the Late Blight Disease

of Potato in Bangladesh . Abstract in the Proceedings of the GILB Conference held at Germany

from 11-13 July, 2002.

Experiment 2: Screening of Potato Varieties and CIP Germplasm Against

Heat Tolerance

M. Dhar, M. Hossain, B.C. Kundu, A.T.M.T. Islam, S. Abdullah, M.H. Rahman, E.H.M.S. Rahaman,

M. M. Hasan and M.S. Kadian

Abstract

Five CIP germplasms, one TCRC improved potato variety and one local variety were evaluated at

Pahartoli, Chittagong during 2009-10 for their suitability against heat tolerance. Among the

improved variety Diamant, local variety Dohazari Sada and all the CIP germplasms found to be

promising against heat tolerance.

10

Introduction

A rise in temperature leads to increased transpiration in the plants, thus raising their demand for

water. In many of the drier potato growing regions this will cause water stress, leading yields to

decline. Temperature has a crucial influence on starch deposition in potato tubers. The ideal

temperature range is between 15 and 18°C. Overnight temperatures above 22°C severely hamper

tuber development. By contrast, the impact of climate change on potato yields is expected to be

favourable in cultivation areas at higher altitudes. In many of these areas the climatic conditions

for potato growing are improving due to the rising temperatures. Winter season in Bangladesh is

vary short November to March. In the later stage temperature raises very fast so potato plants can

not survive in high temperature. So, we have studied the to identify the potato varieties/germplasm

suitable for cultivation under temperature and extensive study on heat-tolerant expression in

potatoes using commercially important potato cultivars aspect in Bangladesh.

Materials and Methods

One improved (Diamant) & local (Dohazari Sada) variety and five promising CIP germplasms

such as LB-1 (391004.18), LB-2 (391046.14), LB-7 (393371.58), LB- 12 (396031.119) and LB-14

(396244.12) were evaluated against heat tolerance at ARS, Pahartali, Chittagong during 2009-10.

Brief of the experiments are shown in table 2.1. Total number of tuber 30 per plot. Fertilizers were

applied @ 325-220-250-120 kg/ha of Urea, TSP, MP and Gypsum, respectively. Full amount of

TSP, MP and Gypsum and 50% of urea were applied as basal and the remaining amount of urea

was side dressed at 35 DAP. Weeding, irrigation, earthing-up and other intercultural operations

were done as per TCRC recommendation. The crop was harvested at 90 DAP. All the yield and

yield contributing characters data were recorded and were analyzed statistically. Plant vigor was

recorded on 1-5 scale, where 1 is very poor vigor and 5 is highly vigorous.

Table 2.1. Experiments in brief of heat tolerance trial at Pahartali, Chittagong during 2009-

10

Plant spacing : 60 cm X 25 cm

Plot size : 1.8 m X 2.0 m

Design : RCB

Replication : 3.00

Plot to plot : 0.60 m

Rep-rep distance : 1.0 m

No of rows per plot : 3

No of tubers per row : 10

Date of planting : 26.12.2009

Date of halm pulling : 15.03.2010

Date of harvesting : 23.03.2010

11

Results and Discussion

All the yield and yield contributing characters of the studied varieties and germplasms varied

significantly. All the varieties showed more than 85% emergence except LB-12 which had the

lowest germination (18.06%). Considering plant vigour, LB-12, Diamant and Dohazri Sada was

the robust while LB-1 & LB-14 showed less vigour but there was no significant variation. Plant

heights at 60 DAP varied significantly and it was ranged from 38.07 to 60.07 cm. Number of stems

per plant at 45 DAP was not varied significance among the studied varieties/germplasm. The local

variety Dohazari Sada was looked as indigenous type and had the highest number of compound

leaves/plant (252.87), while 97-40 to 145.87 was recorded among the CIP germplasms/varieties.

All the CIP germplasms had good number of compound leaves per plants. Percent ground

coverage showed more than 80% except Diamant. There was no significant effect of senescence

percent at 60 DAP but it was varied significance at 67 and 74 DAP of the studied

varieties/germplasm on the heat tolerance trial (Table 2.2).

Table 2.2 Morphological character of heat tolerance trial at Pahartoli, Chittagong

TCRC/CIP

clones/variety

Emergence

(%)

Plant

vigor*

Plant height (cm) at DAP Stems/plant

at 45 DAP 45 60

LB-1(391004.18) 100.00 a 4.67 a 34.00 c 38.07 c 8.80a

LB-2 (391046.14) 92.22 a 4.33 a 41.33 ab 60.07 a 6.93a

LB-7 (393371.58) 98.89 a 4.67 a 42.87 a 52.00 ab 4.47a

LB-14 (396244.12) 95.56 a 4.33 a 45.13 a 54.80 ab 9.67a

LB-12 (396031.119) 21.11 b 5.00 a 25.30 d 45.77 bc 3.48a

Diamant 97.78 a 5.00 a 34.67 bc 38.93 c 8.92a

Dohazari Sada 98.89 a 5.00 a 35.80 bc 54.40 abc 6.80a

CV (%) 7.11 0.76 10.10 14.66 52.72

LSD 0.05 9.70 9.45 6.37 12.27 6.294

Continue

TCRC/CIP clones/variety Compound

leaves/plant

PGC Senescence (%) at DAP

60 67 74

LB-1(391004.18) 97.40 bc 90.00 a 4.67a 2.33c 2.33c

LB-2 (391046.14) 145.87 b 90.00 a 4.33a 3.00bc 3.00bc

LB-7 (393371.58) 88.07 bc 95.00 a 4.67a 3.33ab 3.33ab

LB-14 (396244.12) 139.70 bc 93.33 a 4.33a 3.00bc 3.00bc

LB-12 (396031.119) 101.97 bc 80.00 b 5.00a 3.33ab 3.33ab

Diamant 80.53 c 91.67 a 5.00a 2.33 c 2.33c

Dohazari Sada 252.87 a 96.67 a 5.00a 4.00 a 4.00a

CV (%) 26.43 5.79 9.45 11.04 15.19

LSD 0.05 58.28 8.977 0.76 0.742 0.788

* Plant vigor on 1 to 5 Scale; where 1 is very poor vigor and 5 is highly vigorous

12

Number and weight of marketable and non-marketable tubers per plot showed significant effect

among the studied varieties/germplasm (Table 2.3). The highest number of tubers/plot

(marketable) was recorded in local variety Dohazari Sada (538.67) as the tubers were medium to

very small in size. LB-7 (10.34 kg) was the highest value of total weight of tuber (kg) per plot

followed by LB-1 and Diamant.

Table 2.3 Yield attributes of heat tolerance trial at Pahartoli, Chittagong

TCRC/CIP

clones/variety

Number of tubers/plot Weight of tubers per plot (kg)

Marketable Non-

Marketable

Total Marketable Non-

Marketable

Total

LB-1 (391004.18) 183.33 b 136.33 b 319.67 b 5.60 a 2.26 ab 7.86 ab LB-2 (391046.14) 79.67 c 125.00 b 204.67 bc 5.33 a 2.57 a 7.91 ab

LB-7 (393371.58) 134.67 bc 113.67 b 248.34 bc 7.63 a 2.71 a 10.34 a

LB-14 (396244.12) 119.00 bc 161.33 b 280.33 bc 5.78 a 2.86 a 8.64 ab

LB12(396031.119) 61.00 c 68.67 b 129.67 c 1.11 b 1.23 b 2.34 c

Diamant 129.00 bc 110.33 b 239.33 bc 6.80 a 2.56 a 9.36 ab

Dohazari Sada 538.67 a 467.33 a 1006.00 a 5.67 a 1.84 ab 7.51 b

CV (%) 28.79 39.17 26.85 28.64 29.59 17.69 LSD 0.05 87.25 112.7 157.9 2.64 1.15 2.39

Tubers weight per plant had varied effect among the varieties and highest weight was obtained

from the CIP germplasm LB-7 (561.00 g) followed by improved variety Diamant, LB-12 (499.67

g), while lowest was recorded in Dohazari Sada (343.00 g). Considering average tuber weight, it

was also varied and ranges from 5.56 g (Dohazari Sada) to 49.94 g (LB-12). Average tuber weight

was also found better in LB-7 (45.75 g) and Diamant (44.57 g). Percentage of dry matter had no

significant effect among the variety/germplasm and highest was recorded in LB-14 (20.00 %)

followed by LB-7 (19.00%) & LB-1 (18.33%). Among all the studied varieties/germplasm, LB-7

was the highest yielder (22.98 t/ha) followed by LB-1 & Diamant (20.81 t/ha) which was

significantly similar while local variety Dohazari Sada gave lowest yield (16.68 t/ha). CIP

germplasm LB-7 (22.98 t/ha) was found promising and gave comparatively better yield than

popular variety Diamant (20.81 t/ha) (Table 2.4).

Among the CIP germplasm, oblong to oval tubers was observed in LB-1 and LB-2, while tubers of

LB-7 and LB-14 had a round-oval-oblong-segregated shaped. Over all ranking of the tubers among

the CIP germplasm, LB-1, LB-7 & LB-12 ranked first followed by, LB-2, Dohazari Sada and

Diamant. (Table 2.5). Over all performance of the popular variety Diamant in heat tolerance trial

was not good but further investigation is needed for conclusion. Environmental data were

presented at annexure –A.

13

Table 2.4 Yield attributes of heat tolerance trial at Pahartali, Chittagong

TCRC/CIP

clones/variety

Tubers

wt. /

Plant (g)

Tubers

number

/

Plant

Average

tuber

wt. (g)

%Dry

Matter

Yield (t/ha)

Marketable Non-

Marketable

Total

LB-1 (391004.18) 431.33 ab 15.60 b 28.21 cd 18.33 a 12.33 a 5.03 ab 17.36ab

LB-2 (391046.14) 439.33 ab 12.13 b 37.07 bc 17.67 a 11.67 a 5.72 a 17.39ab

LB-7 (393371.58) 561.00 a 12.47 b 45.75 ab 19.00 a 17.00 a 6.02 a 23.02 a

LB-14 (396244.12) 470.67 ab 19.27 b 24.69 d 20.00 a 13.00 a 6.35 a 19.35ab

LB12(396031.119) 499.67 a 10.07 b 49.94 a 18.00 a 2.33 b 2.74 b 5.07 c

Diamant 507.67 a 11.53 b 44.57 ab 18.00 a 15.00 a 5.70 a 20.07ab

Dohazari Sada 343.00 b 64.17 a 5.56 e 18.67 a 12.67 a 4.09 ab 16.76 b

CV (%) 17.89 37.56 18.09 9.23 28.15 29.61 17.70

LSD 0.05 141.60 13.27 10.38 2.911 5.754 2.568 5.31

Table 2.5 Observations for heat tolerance trial at Chittagong during 2009-10 cropping

season

TCRC/CIP

clones/variety Observations

LB 1 (391004.18) Yellow skin, oblong, fleet eyes, rotten 4%, cut worm 4%, scab 4%, yellow flesh (4*)

LB 2 (391046.14) Yellow skin, flat round to oval, medium to large tubers, fleet eyes, 11% scab, 2%

cutworm, 9% rotten , heat sprout <1%, yellow flesh (3*)

LB 7 (393371.58) Yellow skin, segregated shaped with round-long-flat, fleet & picked eyes, medium to

large tubers, 6% scab, 8% cut worm, 4% rotten, 2% greening, white flesh (4*)

LB12(396031.119) Red skin, round, medium deep eyes, medium to large size tuber, medium size tuber,

<1% scab, 10% cut worm, 6% rotten, yellow flesh (4*)

LB 14 (396244.12) Yellow skin, oblong-oval-round, fleet eyes, medium to large size, 30% rotten, 20%

scab, 18% cutworm, heat sprout 9%, greening 5%, yellow flesh (1*)

Dohazari Sada Mixed with yellow & pink tuber, round, pronounced picked & deep eyes, small to

medium size, less disease and pest, <1% cut worm, <3% rotten, yellow flesh (3*)

Diamant Yellow skin, oblong tuber, fleet eyes, 4% scab, 10% cut worm, 7% rotten, heat

sprout 2%, yellow flesh (3*)

*over all ranking of the tubers was done using 1-5 scale, where 1 is poor quality and 5 is the best

References

Hijmans, R.J. 2003. The effect of climate change on global potato production. American Journal of Potato

Research. 80: 271-280.

Steyn, J.M., H.F. Du Plessis and P.S. Hammes. 1998. A field screening technique for drought tolerance

studies in potatoes. Potato Research. 41: 295-203.

14

Experiment 3: Degeneration Study of Virus Resistance CIP Germplasm in

Bangladesh

M. Hossain, T.K. Dey, B.C. Kundu, A.T.M.T. Islam, S. Abdullah, B.K. Goswami, E.H.M.S.

Rahaman, M.M. Hasan and M.S. Kadian

Abstract

Eleven CIP clones viz. CIP-1, CIP-2, CIP-3, CIP-4, CIP-5, CIP-6, CIP-8, CIP-9, CIP-10, CIP-13

and CIP-14 with as check variety Diamant were evaluated at Jamalpur and Jessore respectively

during 2009-10 for their suitability against virus resistant. The study was done through improved

and ware practices. At Jamalpur the highest tuber yield was obtained from CIP-10 (51.97 t/ha)

with ware practice whereas Diamant (36.87 t/ha) produced with ware practice. At Jessore the

highest yield was obtained from CIP-9 (48.40 t/ha) with improved practice whereas check variety

Diamant (30.63 t/ha) with improved practice. Virus data will be recorded in next season.

Introduction

Potato (Solanum tuberosum) is the most important commercial crop in Bangladesh. Unfortunately,

potato crops suffer from many fungal bacterial and viral diseases in the country. Among the virus

diseases, potato leaf roll virus (PLRV) and potato virus Y (PVY) are the most important. In

Bangladesh reduction of tuber yield due to PLRV and PVY is reported to rise up to 78% and 85 %

respectively (Hossain et al., 1989 and Hossain and Ali 1993). So the present study was undertaken

to estimate the degeneration of CIP clones due to virus diseases.

Materials and Methods

An experiment was conducted at two locations (Jamalpur and Jessore) during Rabi season 2009-

2010 to evaluate the degeneration effect of virus resistance CIP potato germplasms, viz. CIP-1

(CIP 390637.1), CIP-2 (CIP 391846.5), CIP-3 (CIP 394034.7), CIP-4 (CIP 394611.112), CIP-5

(CIP 394613.139), CIP-6 (CIP 395192.1), CIP-8 (CIP 395195.7), CIP-9 (CIP 397006.18), CIP-10

(CIP 397029.21), CIP-13 (CIP 397073.7), and CIP-14 (CIP 397079.6) with a check variety

Diamant for virus registrant. Design was followed RCB and each treatment was replicated six (6)

times with spacing 60 cm X 25 cm. The plot size 1.8 m X 1.25 m, the date of planting 25

December at Jamalpur and 6 December at Jessore, 2009. Distance in between two treatments: 3m

and there five rows of wheat were planted as boarder plant. There were two treatments; T1:

improved practice, T2: ware practice. In improved practices, insecticides admire was sprayed in

15 days interval. In ware practice the insecticide was not sprayed. Fertilizers were applied @ 325-

220-250-120 kg/ha of Urea, TSP, MP and Gypsum, respectively. Full amount of TSP, MP and

Gypsum and 50% of urea were applied as basal and the remaining amount of urea was side dressed

15

at 35 DAP. Weeding, spraying and other intercultural operations were done as and when

necessary. The crop was harvested at 80 DAP. All the yield and yield contributing characters data

were recorded and were statistically analyzed. Plant vigor was recorded on 1-5 scale, where 1 is

very poor vigor and 5 is highly vigorous. Senescence of the crop was recorded on 1 to 5 scale

where, 5=0-20%; 4=21-40%; 3= 41-60%; 2= 61-80% and 1= 81-100%).

Results and discussion

There was a significant difference of the CIP clones towards growth parameter and different yield

contributing characters of potato. The details of the results were presented in 5.1 to 5.3 for

Jamalpur and Table 3.4 to 3.6 for Jessore. Percent of plant emergence, plant vigor (1-5 scale),

senescence, number of tuber per plant, average tuber weight (g), yield t/ha and dry matter percent

(DM%) varied significantly at 90 DAP at Jamalpur and Jessore. At Jamalpur, interaction effects

between clones and managements the tuber production maximum (51.97 t/ha) was obtained from

CIP-10 with ware practiced followed by CIP-8 and CIP-14, the lowest yield recorded from CIP-3

(30.75 t/ha) with ware practiced. At Jessore effect of interaction the maximum yield (48.40 t/ha)

was obtained from CIP-9 with improved practice followed by the CIP-2 and the lowest yield

(19.53 t/ha) was obtained from CIP-13 with ware practiced (Table 3.6).

Table 3.1 Clonals and managements performance of CIP Potato germplasm degeneration

study of virus resistance at Jamalpur in Bangladesh during 2009-10

* Plant vigor on 1 to 5 Scale; 1: Very poor vigor and 5: Highly vigorous

Value in the column having common letter(s) do not differ significantly at 5% level by DMRT

Clones % of plant Emergence

Plant Vigor *

Senescence Number of tuber/plant

Average Tuber weight (g)

Yield t/ha DM (%)

CIP 1 95.56 a 3.33 d 1.83 b 11 a 74.65 de 48.13 ab 14.00 CIP 2 98.33 a 3.33 d 1.83 b 9 bcd 87.99 bcd 50.06 a 15.00 CIP 3 96.11 a 2.75 e 1.17 c 9 cd 59.90 f 34.56 d 17.00 CIP 4 96.67 a 3.50 d 1.5 bc 11 a 60.32 f 43. 48 abc 19.00 CIP 5 87.78 b 4.00 c 1.33 bc 7 def 89.63 bc 41.13 bcd 20.00 CIP 6 95.00 a 4.00 c 1.17 bc 6 fg 114.59 a 43.51 abc 18.00 CIP 8 95.56 a 5.00 a 1.00 cd 8 de 99.78 b 49.95 a 15.00 CIP 9 88.89 b 4.58 ab 1.33 bc 5 g 122.14 a 40.43 bcd 18.00 CIP 10 98.33 a 4.58 ab 1.17 bc 10 ab 80.00 cde 49.95 a 19.00 CIP 13 92.22 ab 4.75 a 1.33 bc 9 bcd 72.25 ef 41.12 bcd 18.00 CIP 14 97.79 a 4.17 bc 1.50 bc 10 abc 72.65 ef 47.18 abc 17.00 Diamant 96.11 a 3.75 cd 3.00 a 7 ef 87.46 ef 39.92 cd 20.00 Management Improved Practice

96.67 ab 4.10 bcde

1.33 bc 9 abcdeg 80.14 fghi 46.35 abcdef -

Ware Practice

93.06 abc 3.86 cdef

1.69 abc 7 fghi 90.09 defgh

41.86 defgh -

CV (%) 7.28 13.03 46.45 22.1 18.15 13.09 - LSD (0.05) 5.57 0.42 0.57 1.50 12.46 4.66 -

16

Table 3.2 Interaction effects between clones and managements performance of CIP Potato

germplasm degeneration study of virus resistance at Jamalpur in Bangladesh

during 2009-10

Clones % of plant

Emergence

Plant

Vigor

Senescence Number of

tuber/plant

Average

Tuber

weight (g)

Yield t/ha

CIP 1 x T1 100.00 a 3.33 de 2.00 bc 13 a 58.63 i 49.71 abc

CIP 1 x T2 91.11 abc 3.33 de 1.67 bcd 8 defghij 90.67 defgh 46.54 abcde

CIP 2 x T1 98.89 ab 3.50 de 1.33 cd 10 bcdef 77.87 fghi 50.89 abc

CIP 2 x T2 97.78 ab 3.17 e 2.33 ab 8 fghij 98.09 cdef 49.22 abc

CIP 3 x T1 95.56 ab 3.00 ef 1.00 d 9 bcdefgh 61.01 i 38.38 gfh

CIP 3 x T2 96.67 ab 2.50 f 1.33 cd 7 efghij 58.80 i 30.75 i

CIP 4 x T1 97.78 ab 3.33 df 1.33 cd 12 ab 59.28 i 46.11 abcdef

CIP 4 x T2 95.56 ab 3.67 cde 1.67 bcd 10 bcde 61.36 i 40.86 defgh

CIP 5 x T1 90.00 bcd 4.00 bcd 1.33 cd 8 efghij 84.47 defgh 43.33 cdefg

CIP 5 x T2 85.56 cd 4.00 bcd 1.33 cd 6 jkl 94.78 cdefg 38.94 efgh

CIP 6 x T1 96.67 ab 4.33 abc 1.00 d 7 ijk 103.83 cd 46.04 abcdef

CIP 6 x T2 93.33 abc 3.67 cde 1.33 cd 5 kl 125.34 ab 40.99 defgh

CIP 8 x T1 94.44 abc 5.00 a 1.00 d 8 efghij 96.88 cdef 48.29 abcd

CIP 8 x T2 96.67 ab 5.00 a 1.00 d 8 fghij 102. 69 cde 51.62 ab

CIP 9 x T1 94.44 abc 4.83 a 1.00 d 6 jkl 112.52 bc 43.94 bcdefg

CIP 9 x T2 83.33 d 4.33 abc 1.67 bcd 4 l 131.76 a 36.91 ghi

CIP 10 x T1 98.89 ab 4.50 ab 1.00 d 11 abcd 77.23 fghi 47.93 abcd

CIP 10 x T2 97.78 ab 4.67 ab 1.33 cd 10 bcdefg 82. 79 efgh 51.97 a

CIP 13 x T1 94.44 abc 5.00 a 1.00 d 10 bcde 71.41 hi 48.48 abcd

CIP 13 x T2 90.00 bcd 4.50 ab 1.67 bcd 7 hijk 73.09 hi 33.75 hi

CIP 14 x T1 100.00 a 4.33 abc 1.00 d 11abc 70.48 hi 50.15 abc

CIP 14 x T2 95.56 ab 4.00 bcd 2.00 bc 9 cdefi 74.83 ghi 44.21 abcdefg

Diamant x T1 98.89 ab 4.00 bcd 3-00 a 7 ghij 88.03 defgh 42.98 cdefg

Diamant x T2 93.33 abc 3.50 de 3-00 a 6 jkl 86.89 defgh 36.87 ghi

CV (%) 7.28 13.03 46.45 22.17 18.15 13.03

LSD (0.05) 7.88 0.60 0.80 2.13 17.63 6.59

Value in the column having common letter(s) do not differ significantly at 5% level by DMRT

Plant vigor on 1 to 5 Scale; 1: Very poor vigor and 5: Highly vigorous

T1= Improved Practice, T2= Ware Practice

17

Table 3.3 Tuber characters of CIP virus resistant clones of 80 DAP at RARS, Jamalpur

during 2009-10

CIP Clones Characters

CIP-1 (CIP 390637.1) Oval tuber, yellow skin, white to creamy flesh, fleet eyes, 1% cracking

and overall uniformity 4

CIP-2 (CIP 391846.5) Oval tuber, yellow skin & flesh, fleet eyes and overall uniformity 4.

CIP-3 (CIP 394034.7) Oblong to oval tuber, red skin, yellow flesh, fleet eyes and overall uniformity 4

CIP-4 (CIP 394611.112) Round tuber, pink skin, yellow flesh, deep eyes and overall uniformity

3

CIP-5 (CIP 394613.139) Flat round tuber, red skin, yellow flesh, deep eyes and overall

uniformity 5

CIP-6 (CIP 395192.1) Round tuber, yellow skin & flesh, fleet eyes and overall uniformity 4.

Cracking 3%

CIP-8 (CIP 395195.7) Oval to flat round tuber, white skin & flesh, fleet eyes and overall

uniformity 5

CIP-9 (CIP 397006.18) Round tuber, yellow skin & flesh, fleet eyes and overall uniformity 3.

Cracking 10-15%.

CIP-10 (CIP 397029.21) Round tuber, white skin & flesh, fleet eyes and overall uniformity 3

CIP-13 (CIP 397073.7) Oblong tuber, yellow skin, white flesh, fleet eyes and overall uniformity

5. Cracking 1%.

CIP-14 (CIP 397079.6) Round tuber, white skin & flesh, deep eyes and overall uniformity 4.

Diamant Oblong tuber, yellow skin & flesh, fleet eyes and overall uniformity 5

Table 3.4 Clonals and managements performance of CIP Potato germplasm degeneration

study of virus resistance at Jessore in Bangladesh during 2009-10

Clones % of plant

Emergence

Plant

Vigor*

Senescence Number of

tuber/plant

Average

Tuber

weight

(g)

Yield

t/ha

CIP-1 (CIP 390637.1) 99.44 ab 4.92 a 4.00 ab 10.46 c 48.96 c 33.73 bc

CIP-2 (CIP 391846.5) 98.89 ab 4.92 a 4.08 ab 10.16 c 53.03 c 35.82 bc

CIP-3 (CIP 394034.7) 100.00 a 5.00 a 3.42 b 14.07 b 30.33 e 26.74 d

CIP-4 (CIP 394611.112) 98.33 ab 4.42 c 3.50 b 15.75 a 31.90 e 33.02 d

CIP-5 (CIP 394613.139) 95.00 b 4.75 ab 2.50 d 10.89 c 47.34 c 33.65 bc

CIP-6 (CIP 395192.1) 98.33 ab 4.92 a 3.17 bc 7.04 d 69.96 ab 32.68 c

CIP-8 (CIP 395195.7) 96.67 ab 5.00 a 2.00 e 8.33 d 67.76 b 37.06 b

CIP-9 (CIP 397006.18) 99.44 ab 5.00 a 2.83 cd 8.56 d 74.87 a 42.14 a

CIP-10 (CIP 397029.21) 100.00 a 5.00 a 2.42 de 10.56 c 49.89 c 32.24 c

CIP-13 (CIP 397073.7) 98.33 ab 5.00 a 3.42 b 10.32 c 38.67 d 25.91 d

CIP-14 (CIP 397079.6) 99.44 ab 4.50 bc 3.42 b 14.84 ab 36.73 de 35.58 bc

Diamant 98.33 ab 3.92 d 4.33 a 8.57 d 47.53 c 26.42 d

Management

Improved Practice 99.26 a 4.75 ab 2.90 ef 13.06 bc 47.85 def 38.32 bc

Ware Practice 97.78 ab 4.81 ab 3.61 cd 8.54 efg 51.64 de 27.51 fg

CV (%) 3.48 6.79 16.69 16.66 15.71 13.14

LSD (0.05) 2.76 0.26 0.16 1.45 6.30 1.25

* Plant vigor on 1 to 5 Scale; 1: Very poor vigor and 5: Highly vigorous

18

Table 3.5 Interaction effects between clones and managements performance of CIP Potato

germplasm degeneration study of virus resistance at Jessore in Bangladesh during

2009-10

Clones % of plant

Emergence

Plant

Vigor

Senescence Number of

tuber/plant

Average

Tuber

weight (g)

Yield t/ha

CIP 1 x T1 100.00 a 4.83 a 3.67 cd 12.15 bcd 48.38 fgh 38.92 bc

CIP 1 x T2 98.89 ab 5.00 a 4.33 abc 8.78 fghi 49.54 g 28.53 gh

CIP 2 x T1 100.00 a 4.83 ab 3.67 cd 11.69 cde 54.13 cde 42.08 b

CIP 2 x T2 97.78 ab 5.00 a 4.50 ab 8.63 fghi 51.92 def 29.57 fgh

CIP 3 x T1 100.00 a 5.00 a 3.17 de 18.77 a 26.35 k 32.59 defg

CIP 3 x T2 100.00 a 5.00 a 3.67 cd 9.38 fgh 34.31 ijk 20.89 jk

CIP 4 x T1 98.89 ab 4.17 ab 3.17 de 18.47 a 30.53 jk 37.26 bcd

CIP 4 x T2 97.78 ab 4.67 ab 3.83 bcd 13.03 bc 33.27 ijk 28.78 fgh

CIP 5 x T1 97.78 ab 4.83 ab 2.17 fg 13.53 bc 43.48 fghi 38.87 bc

CIP 5 x T2 92.23 c 4.67 ab 2.83 ef 8.25 fghi 51.20 def 28.42 gh

CIP 6 x T1 97.78 ab 4.83 ab 2.67 ef 8.33 fghi 68.80 ab 38.24 bc

CIP 6 x T2 98.89 ab 5.00 a 3.68 cd 5.74 j 71.12 ab 27.13 ghi

CIP 8 x T1 98.89 ab 5.00 a 1.67 g 9.56 efg 62.77 bc 39.83 bc

CIP 8 x T2 94.45 bc 5.00 a 2.33 fg 7.11 hij 72.76 a 34.29 cdef

CIP 9 x T1 98.89 ab 5.00 a 2.50 ef 10.08 defg 72.87 a 48.40 a

CIP 9 x T2 100.00 a 5.00 a 3.17 de 7.10 hij 76.87 a 35.89 cde

CIP 10 x T1 100.00 a 5.00 a 2.17 fg 14.27 b 43.18 fghi 38.96 bc

CIP 10 x T2 100.00 a 5.00 a 2.67 ef 6.87 ij 56.60 cd 25.52 hij

CIP 13 x T1 100.00 a 5.00 a 2.83 ef 12.91 bc 38.84 hij 32.30 defg

CIP 13 x T2 96.67 ab 5.00 a 4.00 abc 7.73 ghij 38.50 hij 19.53 k

CIP 14 x T1 100.00 a 4.50 bc 3.17 de 16.68 a 39.58 ghij 41.78 b

CIP 14 x T2 98.89 ab 4.50 bc 3.67 cd 13.01 bc 33.89 ijk 29.39 fgh

Diamant x T1 98.89 ab 4.00 d 4.00 abc 10.32 def 45.36 efgh 30.63 efgh

Diamant x T2 97.78 3.83 d 4.67 a 6.81 ij 49.71 defg 38.92 bc

CV (%) 3.48 6.79 16.69 16.66 15.71 13.14

LSD (0.05) 3.91 0.37 0.62 2.06 8.92 4.94

Plant vigor on 1 to 5 Scale; 1: Very poor vigor and 5: Highly vigorous

T1= Improved Practice, T2= Ware Practice

19

Table 3.6 Tuber characters of CIP virus resistant clones 80 DAP at RARS, Jessore during

2009-10

CIP Clones Characters

CIP-1 (CIP 390637.1) Oblong tuber, yellow skin, creamy flesh, fleet eyes and overall

uniformity 4

CIP-2 (CIP 391846.5) Oblong to oval tuber, yellow skin & flesh, fleet eyes and overall

uniformity 3. Rottage 3%

CIP-3 (CIP 394034.7) Oblong tuber, red skin, creamy flesh, fleet eyes and overall

uniformity 4

CIP-4 (CIP 394611.112) Round tuber, pink skin, yellow flesh, medium deep eyes and overall

uniformity 4

CIP-5 (CIP 394613.139) Round tuber, red skin, creamy flesh, deep eyes and overall

uniformity 4

CIP-6 (CIP 395192.1) Oval tuber, yellow skin & flesh, fleet eyes and overall uniformity 5.

Heat necrosis found

CIP-8 (CIP 395195.7) Oblong tuber, yellow skin, white flesh, fleet eyes and overall

uniformity 4

CIP-9 (CIP 397006.18) Round tuber, white skin, yellow flesh, fleet eyes and overall

uniformity 5

CIP-10 (CIP 397029.21) Round tuber, white skin & flesh, fleet eyes and overall uniformity 3

CIP-13 (CIP 397073.7) Oblong tuber, white skin & flesh, fleet eyes and overall uniformity 5

CIP-14 (CIP 397079.6) Round tuber, white skin & flesh, deep eyes and overall uniformity 3,

1% cracking

Diamant Oblong tuber, yellow skin & flesh, fleet eyes and overall uniformity

4

Conclusion

From the above study it was revealed that improved practice yielded higher than the ware practice.

But as it is an experiment of degeneration study of virus, so virus data will be recorded in next

season.

20

Experiment 4: Screening of Potato Varieties and CIP Germplasms Against

Salt Tolerance

M. Dhar, M. Hossain, B.C. Kundu, M.H. Rahman, E.H.M.S. Rahaman, M. M. Hasan and

M.S. Kadian

Abstract

Seven CIP germplasms, one TCRC improved potato variety Diamant and one local variety

Dohazari Sada were evaluated at Ukhia, Cox’s Bazar, during 2009-10 for their suitability against

salt tolerance. Among the improved variety Diamant, local variety Dohazari Sada and all the

CIP germplasms found to be growing well up to 40 DAP at saline areas. But after this period all of

them died due to very high level of salinity in the soil (8.9 ds/m).

Introduction

A comparison of leaf ion contents, osmotic potential and gas exchange rates indicates that the rate

of Na+ accumulation is very low at high air humidity, because the water-use efficiency had

increased more. The effects of NaCl in high air humidity were compared with those of KCl, which

is taken up more rapidly, and of polyethylene glycol which is not taken up. The results indicate

that the rate of ion accumulation and the time-course of osmotic changes within the plant are

decisive for the onset of damages. A high level in salinity leads to damage plants, thus the yield

must be decrease for the region of south part of Bangladesh like Chittagong, Barisal and Khulna

divisions. In many of these areas the climatic conditions for potato growing are improving due to

the rising salinity. So, we have studied to identify the potato varieties/germplasm suitable for

cultivation in saline areas of Bangladesh.

Materials and Methods

One improved (Diamant) and local variety (Dohazari Sada) with seven CIP germplasms such as

LB-3 (391058.175), LB-4 (393077.159), LB-6 (393280.64), LB-7 (393371.58), LB-8(395011.2),

LB- 12 (396031.119) and LB-14 (396244.12) were evaluated against salt tolerance at Ukhia, Cox’s

Bazar during 2009-10. Brief of the experiments are shown in Table 4.1. Fertilizers were applied @

325-220-250-120 kg/ha of Urea, TSP, MP and Gypsum, respectively. Full amount of TSP, MP and

Gypsum and 50% of urea were applied as basal and the remaining amount of urea was side dressed

at 35 DAP. Weeding, irrigation, earthing-up and other intercultural operations were done as per

TCRC recommendation. All the yield and yield contributing characters data were recorded and

were analyzed statistically. Plant vigor was recorded on 1-5 scale, where 1 is very poor vigor and 5

is highly vigorous. Salinity level of experimental plots soil was analyzed (Table 4.2).

21

Table 4.1 Experiments in brief of salt tolerance trial at at Ukhia, Cox’s Bazar in Bangladesh

during 2009-10

Plant spacing : 60 cm X 25 cm

Plot size : 0.60 m X 2.5 m

Design : RCB

Replication : 3.00

Plot to plot : 0.60 m

Rep-rep distance : 1.0 m

No of rows per plot : 3

No of tubers per row : 10

Total no of tubers required for 3 replications : 30

Date of planting : 10.12.2009

Date of harvesting : 20.01.2010

Table 4.2 Soil salinity level of experiment plots of salt tolerance trial at Ukhia, Cox’s Bazar in

Bangladesh during 2009-10

Conditions Date Salinity level

Before planting 9.12.09 0.287 dS/m

Two weeks after planting 24.12.09 7.38 dS/m

Forty days after planting (damage all plants) 19.0110 8.98 dS/m

At harvest Dated on 26.2.10 1.84 dS/m

Water sample at 40 DAP 19.0110 3.54 dS/m

Results and Discussion

All the growth and yield contributing characters of the studied varieties and germplasms varied

significantly (Table 4.3). The percent of emergence LB-7 (86.67) showed the highest and the

lowest LB-14 (3.33) while the check variety Diamant (70). Considering plant vigour LB-3 and

LB-7 show the highest (3.67) and the lowest LB-14 (0.33) while the Diamant and local variety

Dohazari (3.00) and (3.33) respectively at 30 DAP. Plant heights and other growth parameters

were not recorded due to all plants was damaged at 40 DAP. The soil salinity level was (8.98

dS/m) at 40 DAP that causes the plants were not survived.

22

Table 4.3 Performance of CIP Salt tolerance resistant clones at Ukhia, Cox’s Bazar in

Bangladesh during 2009-10

* Plant vigor on 1 to 5 Scale; 1: Very poor vigor and 5: Highly vigorous

All damaged plants tuber were harvest at 40 DAP, Number and weight of marketable and non-

marketable tubers per plot showed significant effect among the studied varieties/germplasm (Table

4.4). The highest number of tubers/plot was recorded in local variety Dohazari (105) as the tubers

were very small in size. The lowest total number of tuber was obtained from LB-4 (3.67). The total

tuber weight (g) per plot was found from LB-7 (410 g) and the lowest LB-4 (30 g) while Diamant

and Dohazari were (325 g) and (383.33 g) respectively. (Table 4.4).

Table 4.4 Yield attributes of salt tolerance trial at Ukhia, Cox’s Bazar

Clones

Tuber number per plot Tuber weight per plot

Marketable Non

Marketable

Total Marketable Non

Marketable

Total

LB-3 5.67 a 36.67 bc 42.33 bc 143.33 a 236.67 abc 380.00ab

LB-4 0.00 3.67 e 3.67 e 0.00 c 30.00 d 30.00 d

LB-6 3.67 a 31.00 cd 34.67 c 73.33 b 176.67 bcd 250.00 abc

LB-7 3.00 ab 56.67 b 59.67 b 93.33 ab 316.67 ab 410.00 a

LB-8 0.00 b 27.33 cde 27.33 cd 0.00 c 190.00 bcd 190.00 bcd

LB-12 4.00 a 27.33 cde 31.33 cd 126.67 ab 163.33 bcd 290.00 abc

LB-14 0.00 8.33 de 8.33 de 0.00 c 100.00 cd 100.00 cd

Diamant 5.67 a 26.00 cde 31.67 cd 160.00 a 165.00 bcd 325.00 ab

Dohazari 0.00 b 105.00 a 105.00 a 0.00 c 383.33 a 383.33 ab

CV (%) 66.10 35.71 34.87 56.79 49.55 41.88

LSD (0.05) 2.71 22.37 22.37 63.19 162.8 184.2

Clones Emergence (%) % Foliage

Coverage at 30 DAP

* Vigor at 30 DAP

LB-3 (391058.175) 66.67 a 50.00 abc 3.67 a

LB-4 (393077.159) 10.00 c 10.00 d 1.33 bc

LB-6 (393280.64) 76.67 a 36.67 bc 3.00 ab

LB-7 (393371.58) 86.67 a 60.00 a 3.67 a

LB-8 (395011.2) 40.00 a 31.67 c 2.33 ab

LB- 12 (396031.119) 70.00 a 43.33 abc 3.00 ab

LB-14 (396244.12) 3.33 c 0.00 d 0.33 c

Diamant 70.00 a 36.67 bc 3.00 ab

Dohazari 80.00 a 53.33 ab 3.33 a

CV (%) 26.46 31.83 36.04

LSD (0.05) 24.78 19.09 1.59

23

The number of tubers per plant had a significant effect among the CIP germplasms/varieties. The

highest tuber number per plant (13.35) was obtained from the local variety Dohazari followed by

LB-14 and the lowest (1.22) was recorded from LB-4. Tuber weight per plant the highest value

(10.59 g) showed from Daimant followed by LB-12 and the lowest (2.73 g) was recorded from

LB-4. Considering average tuber weight the highest (2.47 g) was recorded from LB-12 followed

by Diamant and the lowest average weight of tuber (0.16 g) from LB-14. Tuber yield were very

negligible which were not countable but LB-14 gave the highest yielded (6.67 t/ha) followed by

LB-3 (3.96 t/ha), LB-8 (3.41 t/ha) which was significantly similar while LB-7 gave the lowest

yield (0.67 t/ha). (Table 4.5).

Table 4.5 Yield attributes of salt tolerance trial at Ukhia, Cox’s Bazar

Clones

Tubers

no./Plant

Tuber wt

(g)/Plant

Avg. Tuber

wt. (g)

Yield (t/ha) at 40 DAP

Marketable Non

Marketable

Total

LB-3 6.65 ab 8.99 ab 1.46 abc 1.51 a 2.45 a 3.96 a

LB-4 1.22 b 2.73 c 0.74 bc 0.00 d 0.67 a 0.67 a

LB-6 4.35 ab 7.37 abc 1.82 ab 0.58 cd 1.53 a 2.11 a

LB-7 6.92 ab 6.65 abc 1.04 bc 0.69 bc 2.44 a 3.14 a

LB-8 7.25 ab 6.91 abc 1.01 bc 0.00 d 3.41 a 3.41 a

LB-12 4.67 ab 9.64 a 2.47 a 1.23 ab 1.60 a 2.84 a

LB-14 8.33 ab 4.00 bc 0.16 c 0.00 d 6.67 a 6.67 a

Diamant 4.47 ab 10.59 a 2.38 a 1.59 a 1.56 a 3.15 a

Dohazari 13.35 a 3.40 c 0.25 c 0.00 d 3.29 a 3.29 a

CV (%) 74.06 40.54 54.81 56.75 141.17 114.00

LSD (0.05) 7.90 4.56 1.16 0.59 6.21 6.21

Conclusion:

In summary, plant growth at 30 DAP very well but at 40 DAP when soil salinity became 8.98

dS/m than plants were not survived so, it will be repeated in next year at low level salinity area of

Bangladesh.

24

Experiment 5: Screening of CIP Clones against Drought Tolerance

M. Hossain, A.A. Mahmud, S. Akhter, B.C. Kundu, M.M. Miah, E.H.M.S. Rahaman, A.T.M.T. Islam,

S. Abdullah, M.M. Hasan and M.S. Kadian

Abstract

Seven and eleven CIP clones with as checked variety Lalpakri, Lalashil and Diamant were

evaluated at Bogra and Dinajpur respectively during 2009-10 for their suitability against drought

tolerance. Most of the CIP clones performed better against drought tolerance than the checks

variety. Significant differences have been reported among the clones/variety and between

irrigation treatments in most traits. In general, as water stress increased, vegetative growth and

tuber yield decreased. Considering yield and yield attributes, the CIP clones LB-7 (34.3 t/ha) with

normal irrigation on the other hand (33.8 t/ha) produced under one irrigation practiced at Bogra

and CIP-101 (47.62 t/ha) and (42.00 t/ha) was higher produced with normal irrigation and one

irrigation which are found promising against drought tolerance.

Introduction

Climate change is expected to intensify the drought events throughout the world, resulting in large-

scale ecosystem alteration and failure of drought-sensitive crops. In addition, periods of drought

vary from year to year in severity and length, making it difficult for plants to adapt to more severe

conditions. Many modern varieties of potatoes are considered to be drought-sensitive. Many of the

ancient potato landraces were adaptations to different environmental conditions such as different

soils, temperature, altitude, and drought (R. Schlafleitner, 2008). The scientists of CIP have been

continuing their efforts to these cultivars to identify the genes and molecular mechanisms of

drought tolerance in order to develop drought-resistant crops of potato. The International potato

Center in Lima, Peru maintains the world's largest collection of tubers in the interest of conserving

the genetic diversity of potato as well as investigating traits such as resistance to various insects

and diseases, as well as to cold, heat and drought. The present experiment was undertaken with

CIP clones, two local cultivars and variety Diamant to evaluate the lines/variety to cope with the

climatic changes in temperature and water availability and struggle to maintain a food supply for

growing populations.

25

Materials and Methods

The experiments were conducted at two locations viz. Bogra and Dinajpur. At Bogra, the trial was

conducted at Tuber Crops Research Sub-Center. At Dinajpur, the same trial was conducted BARC

experimental field under supervision of BARC and the scientists of TCRC and CIP provided the

technical support for conducting these experiments. The protocol of drought tolerance trial has

already been established at CPRI, India and the same protocol was followed in Bangladesh trials.

At Bogra, 7 CIP clones (LB-1, LB-2, LB-3, LB-7, LB-9, LB-12, and LB-14) and at Dinajpur, 11

CIP clones (CIP-101, CIP-102, CIP-111, CIP-117, CIP-124, CIP-126, CIP-129, CIP-130, CIP-134,

CIP-137 and CIP-139) with three checks (Diamant, Lalshil & Lalpakri) were included for drought

tolerance trial. Brief of the experiments are shown in Table 5.1. There were three treatments; T1:

Normal Irrigation (3) as practiced by farmer, T2: One irrigation after 1st earthing up at 30 DAP and

T3: Two irrigations after earthing up at 30 & 60 DAP. Fertilizers were applied @ 325-220-250-120

kg/ha of Urea, TSP, MP and Gypsum, respectively. Full amount of TSP, MP and Gypsum and

50% of urea were applied as basal and the remaining amount of urea was side dressed at 35 DAP.

Weeding, spraying and other intercultural operations were done as and when necessary. The crop

was harvested at 90 DAP. All the yield and yield contributing characters data were recorded and

were statistically analyzed. Plant vigor was recorded on 1-5 scale, where 1 is very poor vigor and 5

is highly vigorous. Senescence of the crop was recorded on 1 to 5 scale where, 5=0-20%; 4=21-

40%; 3= 41-60%; 2= 61-80% and 1= 81-100%). Wilting of plants was also recorded on 1 to 5

scales where, 5 is fully expanded/no wilting and 1 is fully wilted.

Table 5.1 Experiments in brief of drought tolerant trials of CIP clones at two

locations in Bangladesh during 2009-10

Experiment Details Locations

Bogra Dinajpur

Plant spacing 60 cm X 20 cm 60 cm X 20 cm

Plot size 1.8 m X 2.0 m 0.60 m X 2.0 m

Design STRIP PLOT STRIP PLOT

Replication 3 3

Distance between replication 1 m 1 m

Distance between plot 0.60 m 0.60 m

Distance between treatments 3 m 3 m

Date of planting 25.11.09 20.11.09

Date of haulm pulling 16.02.10 20.02.10

Date of harvesting 26.02.10 27.02.10

26

Results and Discussion

Vegetative Parameters: The growth and vegetative characters of the studied CIP clones and

varieties were analyzed and presented in the Table (5.2 to 5.5). Both Bogra and Dinajpur, there

were varied significant effect of irrigation on plant emergence percent, number of stems/plant. At

Bogra the highest emergence percent (100) were showed in LB-7 and Diamant. At Dinajpur plant

emergence percent were maximum clones showed in all treatments. At Dinajpur the percent ground

covers at 60 DAP the highest value (94.7) was recorded from CIP-134 with normal irrigation and

lowest (67.7) was found in Lalshil with two irrigations treatment. (Table 5.3). There was varied

significant effect of irrigation and clones/variety towards plant vigor and plant height at 45, 60 and

75 DAP (Table 5.4 & 5.5). At Bogra LB-2, LB-3 and LB-14 were showed the highest value (5.00)

in all treatments. On the other hand Lalshil was the very poor vigor in all treatments. At Dinajpur,

interaction between irrigation and clones/variety on plant vigor the highest value (5.00) was

observed from CIP-102, CIP-111, CIP-117 and CIP-129 with all treatments. Whereas check variety

Diamant, Lalshil and Lalpakri were the lowest average value 2.87, 2.70 and 3.30 respectively.

Yield parameters: The yield and yield contributing characters of the studied CIP clones and

varieties were analyzed and presented in the Table (5.6 to 5.13). At Bogra and Dinajpur number of

marketable, non-marketable and total tubers per plot varied significantly when different irrigation

schedule and clones/variety were used (Table 5.6 & 5.7). Irrigation, variety and their interaction

had a significant effect towards weight of marketable, non-marketable and total tubers per plot

both at Bogra and Dinajpur (Table 5.8 & 5.9). In case of tubers number per plant at Bogra, number

of tuber per plant Lalshil was the highest 23.9, 23.7 and 25.0 with all treatments respectively and

the lowest average 6.28 with all treatments found in Diamant (Table 5.10). At Dinajpur, higher

number of tubers per plant was obtained from Lalpakri in normal irrigation 32.8 tubers/plant

followed by two irrigation (20.2 tubers/plant) while one irrigation gave 26.7 tubers/plant (Table

5.11). Irrigation, clones/variety and their interaction showed significant effect on average tuber

weight both at Bogra and Dinajpur. At Bogra, normal irrigation gave highest average tuber weight

LB-14 (77.5g) followed by single irrigation (80.6g) while two irrigations gave (109.2g) where the

lowest was recorded in two irrigation (Table 5.10). At Dinajpur there were significant difference

observed (Table 5.11). At Bogra LB-14 gave the highest average tuber weight (109.2g) with two

irrigations treatment followed by LB-9 (56.5g) in one irrigation LB-14 gave average tuber weight

(80.6 g) followed by LB-9 (42.4g) and in normal irrigation treatment the LB-14 (77.5 g) but

Lalpakri and Lalshil produced very smaller tubers (Table 5.10) then the other clones. There were

significant effect in average tuber weight, tubers weight per plant both at Bogra and Dinajpur

(Table 10 & 11). At Bogra, the highest tuber weight per plant LB-7 (520.2g) was recorded when

normal irrigation practices were followed which was significantly different from two irrigations

27

(439.8g) while, one irrigation it was gave (507.0g). At Dinajpur in normal irrigation CIP-101 &

CIP-134 were gave the highest (710.0g) tubers per plant where they produced in one irrigation

(630.0g & 480.0 g) tubers/plant respectively. Form two irrigation they produced 660.0g & 670g

(Table 5.11). At Bogra among the clones, LB-7 yielded the highest (34.3 t/ha) in normal irrigation

and in two irrigations (29.3 t/ha) where single irrigation (33.8 t/ha) followed by LB-9 (33.3 t/ha)

and LB-2 (32.3 t/ha) (Table 5.12). At Dinajpur CIP-101 was produced higher yield (47.62 t/ha) in

normal irrigation, in two irrigation (43.73 t/ha) while single irrigation (42.0 t/ha) whereas check

variety Diamant was recoded in single irrigation treatment was produced (23.67 t/ha) similarly

Lalshil and Lalpakri produced very little yield (8.78 t/ha) & (16.80 t/ha) respectively (Table 5.13).

Regarding all growth and yield measurements of the two locations, the CIP clones LB-7

(393371.58) at Bogra and at Dinajpur CIP-101, CIP-117 and CIP-126 found potential against

drought tolerance. Environmental data were presented at annexure –A.

Reference

R. Schlafleitner. 2008. Drought tolerance in potatoes. Published: Saturday, June 28, 2008 -11:21 in

Biology & Nature.

Source: American Society of Plant Biologists.

Contact: Roland Schlafleitner; [email protected]; 511-349-6017

28

Table 5.2 Effect of irrigation and clones/varieties on growth characters of potato

Bogra

TCRC/CIP

clones/variety

Emergence (%) Mean

Stem Number/Plant

at 45 DAP Mean

Percent Ground

Cover (PGC) at

60 DAP Mean

T1 T2 T3 T1 T2 T3 T1 T2 T3

LB 1 (391004.18) 97.8 96.7 98.9 97.78 4.4 3.7 3.4 3.83 97.3 93.4 98.4 96.40

LB 2 (391046.14) 100.0 91.1 96.7 95.93 5.3 4.5 3.1 4.32 100.0 99.3 99.9 99.74

LB 3 (391058.175) 98.9 98.9 100.0 99.26 4.3 4.1 3.3 3.91 97.6 99.0 98.3 98.30

LB 7 (393371.58) 98.9 100.0 100.0 99.63 3.7 3.8 3.1 3.53 99.0 99.1 99.2 99.11

LB 9 (396004.337) 100.0 98.9 98.9 99.26 4.8 4.2 3.6 4.18 95.0 97.5 97.1 96.52

LB 12 (396031.119) 98.9 93.3 98.9 97.04 3.9 3.6 3.7 3.76 98.7 95.3 96.8 96.93

LB 14 (396244.12) 67.8 88.9 92.2 82.96 4.2 3.9 3.3 3.78 97.0 98.4 99.2 98.22

Diamnat 100.0 100.0 97.8 99.26 4.8 3.9 3.2 4.00 98.9 97.2 98.3 98.15

Lalshil 97.8 97.8 100.0 98.52 5.0 4.1 3.7 4.28 90.1 89.0 93.6 90.89

Lalpakri 100.0 98.9 100.0 99.63 4.9 3.8 3.6 4.08 97.9 95.8 99.0 97.56

Mean 96.0 96.4 98.3 4.5 4.0 3.4 97.1 96.4 98.0

Statistics SEm ± CD0.05 SEm ± CD0.05 SEm ± CD0.05

Irrigation (I) 1.59 2.36 0.51 0.29 1.49 0.44

Clone/variety(V) 5.86 4.31 0.37 0.52 2.05 2.27

Interaction I×V 3.07 7.47 0.23 0.27 1.62 1.27

T1: Normal Irrigation; T2: One Irrigation; T3: Two Irrigation (1st at earthing and 2nd after 30 days)

29

Table 5.3 Effect of irrigation and clones/varieties on growth characters of potato

Dinajpur

TCRC/CIP

clones/variety

Emergence (%) Mean

Stem

Number/Plant at

45 DAP Mean

Percent Ground

Cover

(PGC) at 60 DAP Mean

T1 T2 T3 T1 T2 T3 T1 T2 T3

CIP-101(301024.14) 100.0 96.7 100.0 98.9 2.3 2.3 2.3 2.29 87.3 82.3 68.7 79.44

CIP-102 (301029.18) 96.7 100.0 100.0 98.9 1.5 1.9 1.9 1.76 86.0 83.3 70.7 80.00

CIP-111 (380583.8) 100.0 96.7 100.0 98.9 2.1 1.7 1.7 1.82 84.0 79.3 70.7 78.00

CIP-117 (386292.3) 100.0 96.7 100.0 98.9 2.9 2.3 2.7 2.64 86.7 87.7 71.3 81.89

CIP-124 (392781.1) 100.0 100.0 100.0 100.0 3.9 3.3 3.3 3.51 83.0 83.3 71.3 79.22

CIP-126 (392797.22) 100.0 100.0 100.0 100.0 4.0 3.6 4.1 3.91 87.7 81.3 75.0 81.33

CIP-129 (393536.13) 100.0 100.0 100.0 100.0 3.2 3.7 4.1 3.67 89.0 82.7 70.7 80.78

CIP-130 (393617.1) 93.3 96.7 96.7 95.6 2.7 3.9 3.6 3.40 90.7 87.0 76.7 84.78

CIP-134 (395183.7) 100.0 100.0 100.0 100.0 3.6 4.1 4.1 3.93 94.7 88.7 77.3 86.89

CIP-137 (395193.6) 96.7 96.7 96.7 96.7 3.1 3.8 5.4 4.09 92.3 87.3 81.3 87.00

CIP-139 (396311.1) 100.0 100.0 100.0 100.0 2.5 2.3 2.7 2.49 83.0 75.7 78.3 79.00

Diamant 96.7 100.0 100.0 98.9 5.8 3.2 2.9 3.98 81.3 84.0 78.0 81.11

Lalshil 100.0 100.0 90.0 96.7 4.6 5.3 4.1 4.67 83.7 82.7 67.7 78.00

Lalpakri 100.0 100.0 100.0 100.0 4.7 3.7 3.1 3.80 77.0 75.7 69.7 74.11

Mean 98.8 98.8 98.8 3.3 3.2 3.3 86.2 82.9 73.4

Statistics SEm ± CD0.05 SEm ± CD0.05 SEm ± CD0.05

Irrigation (I) 1.41 2.01 0.55 0.53 3.38 1.02

Clone/variety (V) 5.73 3.93 2.08 1.14 1.95 2.21

Interaction I × V 3.37 8.34 0.81 1.97 1.58 3.83

T1: Normal Irrigation; T2: One Irrigation; T3: Two Irrigation (1st at earthing and 2nd after 30 days)

30

Table 5.4 Effect of irrigation and clones/varieties on plant vigor and plant height of potato

Bogra

TCRC/CIP

clones/variety

Plant Vigor *

(50 DAP) Mean

Plant height (cm)

45 DAP Mean

60 DAP Mean

75 DAP Mean

T1 T2 T3 T1 T2 T3 T1 T2 T3 T1 T2 T3

LB 1 (391004.18) 4.0 4.7 4.3 4.33 45.5 39.0 50.3 44.91 50.2 61.9 49.9 53.98 51.3 52.5 52.7 52.20

LB 2 (391046.14) 5.0 5.0 5.0 5.00 52.2 44.5 53.5 50.09 62.3 59.3 64.7 62.09 59.5 64.1 59.7 61.11

LB 3 (391058.175) 5.0 5.0 5.0 5.00 53.3 44.2 52.0 49.82 57.4 64.2 54.5 58.69 57.7 61.7 60.6 60.00

LB 7 (393371.58) 5.0 5.0 5.0 5.00 53.5 47.9 51.9 51.09 56.0 67.5 57.5 60.31 58.1 62.0 55.9 58.67

LB 9 (396004.337) 5.0 4.7 4.3 4.67 49.8 42.9 49.5 47.40 44.9 52.4 50.9 49.40 53.1 54.1 53.9 53.71

LB 12 (396031.119) 5.0 4.7 4.7 4.78 50.6 40.3 52.2 47.70 55.4 69.1 63.0 62.51 57.9 61.6 59.1 59.53

LB 14 (396244.12) 5.0 5.0 5.0 5.00 55.2 46.9 54.8 52.29 62.8 70.8 67.5 67.02 57.7 67.5 64.0 63.05

Diamnat 5.0 5.0 4.0 4.67 48.3 43.1 46.7 46.04 49.4 53.2 50.9 51.18 51.0 54.1 52.8 52.62

Lalshil 2.7 3.0 2.3 2.67 37.3 33.5 36.9 35.89 60.0 68.8 61.7 63.49 56.9 65.5 65.4 62.60

Lalpakri 4.3 4.3 3.0 3.89 40.3 36.2 34.6 37.02 47.0 48.7 48.6 48.12 54.0 54.7 51.8 53.49

Mean 4.6 4.6 4.3 48.6 41.9 48.2 54.5 61.6 56.9 55.7 59.8 57.6

Statistics S Em ± CD0.05 SEm ± CD0.05 SEm ± CD0.05 S Em ± CD0.05

Irrigation (I) 0.27 0.18 13.12 1.89 4.39 3.13 1.63 1.84

Clone/variety (V) 0.19 0.32 2.91 3.45 5.96 5.73 2.37 3.35

Interaction I × V 0.23 0.18 2.46 1.89 4.08 9.93 2.38 5.80

T1: Normal Irrigation; T2: One Irrigation; T3: Two Irrigation (1st at earthing and 2nd after 30 days)

*Plant vigour (1-5 scale), where 1 is weakest and 5 is robust

31

Table 5.5 Effect of irrigation and clones/varieties on plant vigor and plant height of potato

Dinajpur

TCRC/CIP

clones/variety

Plant vigour *

(45 DAP) Mean

Plant height (cm) at

45 DAP Mean

60 DAP Mean

75 DAP Mean

T1 T2 T3 T1 T2 T3 T1 T2 T3 T1 T2 T3

CIP-101(301024.14) 4.0 4.7 4.3 4.33 56.6 43.9 38.6 46.38 77.4 62.7 51.1 63.73 85.0 70.3 70.3 75.18

CIP-102 (301029.18) 5.0 5.0 5.0 5.00 25.8 31.7 26.3 27.93 55.1 61.9 45.5 54.18 62.7 69.5 69.5 67.22

CIP-111 (380583.8) 5.0 5.0 5.0 5.00 38.9 41.3 36.2 38.78 75.2 71.4 59.7 68.76 82.8 79.0 79.0 80.27

CIP-117 (386292.3) 5.0 5.0 5.0 5.00 42.6 39.7 29.4 37.22 64.2 61.6 43.1 56.29 71.8 69.2 69.2 70.07

CIP-124 (392781.1) 5.0 4.7 4.3 4.67 41.1 44.9 34.4 40.15 61.5 64.5 47.4 57.80 69.1 72.1 72.1 71.11

CIP-126 (392797.22) 5.0 4.7 4.7 4.80 45.5 47.0 34.3 42.25 75.6 68.7 60.8 68.38 83.2 76.3 76.3 78.62

CIP-129 (393536.13) 5.0 5.0 5.0 5.00 43.1 39.3 32.5 38.29 71.4 64.0 55.1 63.51 79.0 71.6 71.6 74.07

CIP-130 (393617.1) 5.0 5.0 4.0 4.67 48.0 43.9 34.2 42.04 75.5 67.3 49.4 64.07 83.1 74.9 74.9 77.64

CIP-134 (395183.7) 3.7 3.0 4.3 3.67 54.7 44.9 41.7 47.11 72.7 70.7 60.3 67.93 80.3 78.3 78.3 79.00

CIP-137 (395193.6) 4.3 4.3 3.6 4.07 51.0 42.9 60.9 51.58 72.4 66.5 56.7 65.20 80.0 74.1 74.1 76.09

CIP-139 (396311.1) 4.3 4.1 3.9 4.10 36.0 43.9 36.9 38.91 59.9 71.3 62.3 64.49 67.5 78.9 78.9 75.11

Diamant 3.1 2.6 2.9 2.87 49.1 42.4 33.4 41.62 65.7 64.7 50.1 60.16 73.3 72.3 72.3 72.62

Lalshil 2.7 2.3 3.1 2.70 42.7 40.3 36.7 39.93 75.4 69.8 61.6 68.93 83.0 77.4 77.4 79.27

Lalpakri 3.1 3.5 3.3 3.30 40.3 37.0 31.5 36.27 67.5 59.7 47.9 58.36 75.1 67.3 67.3 69.89

Mean 4.3 4.2 4.2 44.0 41.6 36.2 69.3 66.1 53.6 76.9 73.7 73.7

Statistics SEm ± CD0.05 SEm ± CD0.05 SEm ± CD0.05 SEm ± CD0.05

Irrigation (I) 0.23 0.33 6.47 4.00 2.23 3.30 5.29 0.32

Clone/variety (V) 0.17 0.47 8.28 8.66 6.19 7.19 40.66 0.69

Interaction I × V 0.21 0.27 6.19 14.99 5.14 12.46 28.03 0.97

T1: Normal Irrigation; T2: One Irrigation; T3: Two Irrigation (1st at earthing and 2nd after 30 days)

*Plant vigour (1-5 scale), where 1 is weakest and 5 is robust

32

Table 5.6 Effect of irrigation and clones/varieties on tubers number per plot

Bogra

TCRC/CIP

clones/variety

No. of Marketable

Tubers/Plot Mean

No. of Non-Marketable

Tubers/Plot Mean No. of Total Tubers/Plot

Mean

T1 T2 T3 T1 T2 T3 T1 T2 T3

LB 1 (391004.18) 180.3 200.0 219.0 199.77 48.7 65.7 43.7 52.67 229.0 265.7 262.7 252.47

LB 2 (391046.14) 220.3 179.3 199.0 199.53 72.0 73.7 57.3 67.67 292.3 253.0 256.3 267.20

LB 3 (391058.175) 160.0 137.0 144.0 147.00 63.3 66.3 54.7 61.44 223.3 203.3 198.7 208.43

LB 7 (393371.58) 206.0 207.3 198.3 203.87 50.7 75.7 51.7 59.34 256.7 283.0 250.0 263.23

LB 9 (396004.337) 196.0 160.0 173.3 176.43 53.0 45.0 43.0 47.00 249.0 205.0 216.3 223.43

LB 12 (396031.119) 198.0 194.3 211.7 201.33 64.3 74.7 73.0 70.67 262.3 269.0 284.7 272.00

LB 14 (396244.12) 138.7 138.0 104.7 127.13 23.7 37.3 36.0 32.33 162.3 175.3 140.7 159.43

Diamnat 150.3 144.7 137.7 144.23 45.7 49.7 33.0 42.78 196.0 194.3 170.7 187.00

Lalshil 306.0 258.3 329.7 298.00 393.3 435.7 420.7 416.57 699.3 694.0 750.3 714.53

Lalpakri 405.3 346.7 308.0 353.33 224.7 200.7 236.3 220.57 630.0 547.3 544.3 573.87

Mean 216.1 196.6 202.5 103.9 112.4 104.9 320.0 309.0 307.5

Statistics S Em ± CD0.05 S Em ± CD0.05 S Em ± CD0.05

Irrigation (I) 47.07 25.37 58.56 24.15 42.00 29.20

Clone/variety (V) 29.73 46.31 32.66 44.09 50.22 53.31

Interaction I × V 32.96 80.21 31.38 76.37 37.94 92.33

T1: Normal Irrigation; T2: One Irrigation; T3: Two Irrigation (1st at earthing and 2nd after 30 days)

33

Table 5.7 Effect of irrigation and clones/varieties on tubers number per plot

Dinajpur

TCRC/CIP

clones/variety

No. of Marketable Tubers/Plot Mean

No. of Non-Marketable

Tubers/Plot Mean No. of Total Tubers/Plot

Mean

T1 T2 T3 T1 T2 T3 T1 T2 T3

CIP-101(301024.14) 83.7 82.0 92.7 86.11 16.7 11.0 25.7 17.78 100.3 93 118.3 103.87

CIP-102 (301029.18) 49.7 47.7 39.3 45.56 15.7 10.7 21.0 15.78 65.33 58.33 60.33 61.33

CIP-111 (380583.8) 57.0 59.7 62.0 59.56 21.7 23.7 35.7 27.00 78.67 83.33 97.67 86.56

CIP-117 (386292.3) 79.0 63.7 66.3 69.67 17.0 18.0 28.7 21.22 96 81.67 95 90.89

CIP-124 (392781.1) 57.0 51.0 65.0 57.67 11.0 9.7 18.7 13.11 68 60.67 83.67 70.78

CIP-126 (392797.22) 56.0 49.7 49.3 51.67 10.7 4.3 10.3 8.44 66.67 54 59.67 60.11

CIP-129 (393536.13) 43.0 38.7 52.7 44.78 5.7 8.3 16.7 10.22 48.67 47 69.33 55.00

CIP-130 (393617.1) 66.0 63.3 92.7 74.00 21.7 22.7 36.0 26.78 87.67 86 128.7 100.79

CIP-134 (395183.7) 89.3 63.0 76.0 76.11 17.0 18.0 14.3 16.44 106.3 81 90.33 92.54

CIP-137 (395193.6) 73.3 36.7 59.0 56.33 11.7 15.3 28.0 18.33 85 52 87 74.67

CIP-139 (396311.1) 58.0 45.0 53.3 52.11 18.0 22.0 19.0 19.67 76 67 72.33 71.78

Diamant 95.3 49.7 70.3 71.78 20.0 23.0 26.0 23.00 115.3 72.67 96.33 94.77

Lalshil 159.7 148.3 118.3 142.10 95.0 95.3 69.0 86.44 254.7 243.7 187.3 228.57

Lalpakri 240.0 119.3 198.0 185.77 87.7 83.0 69.3 80.00 327.7 202.3 267.3 265.77

Mean 86.2 65.5 78.2 26.4 26.1 29.9 112.594 91.6193 108.09

Statistics SEm ± CD0.05 SEm ± CD0.05 SEm ± CD0.05

Irrigation (I) 10.81 7.70 9.46 4.93 13.29 8.95

Clone/variety (V) 21.25 16.64 5.37 3.81 22.98 19.33

Interaction I × V 11.89 28.82 7.61 18.45 13.81 33.47

T1: Normal Irrigation; T2: One Irrigation; T3: Two Irrigation (1st at earthing and 2nd after 30 days)

34

Table 5.8 Effect of irrigation and clones/varieties on weight of tubers per plot

Bogra

TCRC/CIP

clones/variety

Weight of Marketable

Tubers/Plot (kg) Mean

Weight of Non-Marketable

Tubers/Plot (kg) Mean

Weight of Total Tubers/Plot

(kg) Mean

T1 T2 T3 T1 T2 T3 T1 T2 T3

LB 1 (391004.18) 11.1 9.4 11.2 10.59 0.5 0.8 0.4 0.59 11.7 10.3 11.6 11.18

LB 2 (391046.14) 13.8 11.7 12.5 12.68 0.8 1.0 0.5 0.73 14.6 12.7 13.0 13.41

LB 3 (391058.175) 11.0 9.9 10.1 10.33 0.8 0.7 0.6 0.69 11.8 10.6 10.6 11.03

LB 7 (393371.58) 14.9 14.3 12.7 13.96 0.6 0.9 0.5 0.65 15.4 15.2 13.2 14.61

LB 9 (396004.337) 14.4 12.2 10.6 12.41 0.6 0.6 1.7 0.95 15.0 12.8 12.3 13.36

LB 12 (396031.119) 11.4 10.5 10.8 10.88 0.6 0.5 0.5 0.51 12.0 11.0 11.2 11.40

LB 14 (396244.12) 12.1 13.0 13.3 12.77 0.3 0.5 0.4 0.39 12.4 13.4 13.7 13.16

Diamnat 12.2 10.7 9.0 10.66 0.6 0.6 0.4 0.55 12.9 11.3 9.4 11.20

Lalshil 3.3 2.8 3.8 3.29 1.0 1.1 1.2 1.10 4.3 3.8 5.1 4.39

Lalpakri 7.3 5.9 5.7 6.27 0.7 0.9 0.9 0.84 8.0 6.8 6.6 7.11

Mean 11.1 10.1 10.0 0.6 0.8 0.7 11.8 10.8 10.7

Statistics S Em ± CD0.05 S Em ± CD0.05 S Em ± CD0.05

Irrigation (I) 1.98 0.72 0.46 0.25 2.08 0.79

Clone/variety (V) 1.32 1.31 0.29 0.46 1.33 1.43

Interaction I × V 0.94 2.27 0.33 0.79 1.08 2.49

T1: Normal Irrigation; T2: One Irrigation; T3: Two Irrigation (1st at earthing and 2nd after 30 days)

35

Table 5.9 Effect of irrigation and clones/varieties on weight of tubers per plot

Dinajpur

TCRC/CIP

clones/variety

Weight of Marketable Tubers/

Plot (kg) Mean

Weight of Non-Marketable

Tubers/Plot (kg) Mean

Weight of Total Tubers/Plot

(kg) Mean

T1 T2 T3 T1 T2 T3 T1 T2 T3

CIP-101(301024.14) 7.0 5.9 6.4 6.43 0.2 0.2 0.1 0.16 7.1 6.1 6.6 6.59

CIP-102 (301029.18) 4.3 4.6 3.2 4.03 0.1 0.2 0.5 0.26 4.4 4.7 3.7 4.30

CIP-111 (380583.8) 5.3 4.0 4.0 4.44 0.3 0.5 1.1 0.65 5.6 4.5 5.1 5.08

CIP-117 (386292.3) 5.9 5.5 4.9 5.41 0.2 0.3 0.5 0.32 6.1 5.8 5.4 5.74

CIP-124 (392781.1) 5.7 4.8 5.0 5.16 0.1 0.1 0.3 0.20 5.8 5.0 5.3 5.36

CIP-126 (392797.22) 6.5 6.3 6.5 6.44 0.1 0.1 0.3 0.17 6.6 6.4 6.9 6.60

CIP-129 (393536.13) 3.9 3.3 3.8 3.67 0.1 0.3 0.6 0.30 4.0 3.6 4.3 3.98

CIP-130 (393617.1) 5.1 4.5 4.6 4.74 0.1 0.2 0.7 0.33 5.2 4.7 5.3 5.07

CIP-134 (395183.7) 6.9 4.7 6.0 5.83 0.2 0.1 0.7 0.33 7.0 4.8 6.7 6.16

CIP-137 (395193.6) 4.7 2.6 3.5 3.59 0.2 0.1 1.0 0.46 4.9 2.7 4.5 4.05

CIP-139 (396311.1) 4.8 4.0 4.2 4.34 0.2 0.2 0.5 0.29 5.1 4.2 4.6 4.62

Diamant 5.4 2.8 3.9 4.06 0.3 0.7 1.3 0.75 5.7 3.6 5.2 4.81

Lalshil 1.7 1.1 1.3 1.36 0.2 0.2 0.3 0.25 2.0 1.3 1.6 1.61

Lalpakri 3.3 2.2 3.1 2.88 0.3 0.4 0.4 0.36 3.6 2.5 3.6 3.23

Mean 5.0 4.0 4.3 0.2 0.3 0.6 5.2 4.3 4.9

Statistics SEm ± CD0.05 SEm ± CD0.05 SEm ± CD0.05

Irrigation (I) 0.45 0.12 0.24 0.10 0.48 0.31

Clone/variety (V) 0.53 0.87 0.21 0.22 0.55 0.67

Interaction I × V 0.51 0.71 0.16 0.14 0.57 1.17

T1: Normal Irrigation; T2: One Irrigation; T3: Two Irrigation (1st at earthing and 2nd after 30 days)

36

Table 5.10 Effect of irrigation and clones/varieties on number & weight of tubers

Bogra

TCRC/CIP clones/variety Tubers no. /Plant

Mean

Average Tuber Weight

(g) Mean

Tubers wt./Plant (g)

Mean

T1 T2 T3 T1 T2 T3 T1 T2 T3

LB 1 (391004.18) 7.8 9.1 8.9 8.60 50.7 39.6 44.7 44.97 397.5 352.4 390.1 379.98

LB 2 (391046.14) 9.7 9.2 8.8 9.27 49.8 50.1 51.1 50.35 485.0 462.4 448.1 465.18

LB 3 (391058.175) 7.5 6.9 6.6 7.00 52.9 52.0 53.6 52.82 397.7 359.2 354.4 370.44

LB 7 (393371.58) 8.6 9.4 8.3 8.80 60.3 53.5 53.4 55.73 520.2 507.0 439.8 489.01

LB 9 (396004.337) 8.3 6.9 7.3 7.50 60.3 62.4 56.5 59.75 499.8 432.0 414.4 448.74

LB 12 (396031.119) 8.9 9.5 9.6 9.32 45.9 41.4 40.4 42.58 403.7 389.3 377.8 390.25

LB 14 (396244.12) 7.9 6.5 5.2 6.50 77.5 80.6 109.2 89.11 607.2 509.9 496.3 537.79

Diamnat 6.5 6.5 5.8 6.28 65.4 58.2 55.3 59.66 428.6 377.6 322.2 376.11

Lalshil 23.9 23.7 25.0 24.20 6.5 5.6 6.7 6.26 144.9 130.9 168.9 148.24

Lalpakri 21.0 18.4 18.1 19.20

12.7 12.5 12.5 12.54 266.4 228.9 218.6 237.97

Mean 11.0 10.6 10.4 48.2 45.6 48.3 415.1 375.0 363.1

Statistics S Em ± CD0.05 S Em ± CD0.05 S Em ± CD0.05

Irrigation (I) 1.48 0.34 6.45 6.51 66.56 3.56

Clone/variety (V) 1.66 1.77 5.27 11.89 4.71 1.77

Interaction I × V 1.26 3.06 8.46 20.59 15.54 3.56

T1: Normal Irrigation; T2: One Irrigation; T3: Two Irrigation (1st at earthing and 2nd after 30 days)

37

Table 5.11 Effect of irrigation and clones/varieties on number & weight of tubers

Dinajpur

TCRC/CIP clones/variety Tubers Number /Plant

Mean

Average Tuber Weight

(g) Mean Tuber wt./Plant (g)

Mean

T1 T2 T3 T1 T2 T3 T1 T2 T3

CIP-101(301024.14) 10.0 9.7 11.8 10.51 70 60 60 63.33 710 630 660 666.67

CIP-102 (301029.18) 6.8 5.8 6.0 6.21 70 80 60 70.00 460 470 370 433.33

CIP-111 (380583.8) 7.9 8.6 9.8 8.76 70 60 50 60.00 560 470 510 513.33

CIP-117 (386292.3) 9.6 8.4 9.5 9.18 60 70 60 63.33 610 600 540 583.33

CIP-124 (392781.1) 6.8 6.1 8.4 7.08 90 90 70 83.33 580 500 530 536.67

CIP-126 (392797.22) 6.7 5.4 6.0 6.01 100 120 120 113.33 660 640 690 663.33

CIP-129 (393536.13) 4.9 4.7 6.9 5.50 80 80 60 73.33 400 360 430 396.67

CIP-130 (393617.1) 9.4 8.8 13.4 10.52 60 60 40 53.33 560 490 550 533.33

CIP-134 (395183.7) 10.6 8.1 9.0 9.26 70 60 70 66.67 710 480 670 620.00

CIP-137 (395193.6) 8.8 5.4 9.0 7.76 60 50 50 53.33 510 290 470 423.33

CIP-139 (396311.1) 7.6 6.7 7.2 7.18 70 60 80 70.00 510 420 470 466.67

Diamant 12.0 7.3 9.6 9.62 50 50 50 50.00 590 360 520 490.00

Lalshil 25.5 24.4 21.3 23.71 10 10 10 10.00 200 130 180 170.00

Lalpakri 32.8 20.2 26.7 26.58 10 10 10 10.00 360 250 360 323.33

Mean 11.4 9.3 11.1 62.1 61.4 56.4 530.0 435.0 496.4

Statistics SEm ± CD0.05 SEm ± CD0.05 S Em ± CD0.05

Irrigation (I) 1.38 1.02 0.00 0.00 0.05 0.03

Clone/variety (V) 2.27 2.19 0.00 0.00 0.06 0.07

Interaction I × V 1.57 3.80 0.00 0.00 0.05 0.13

T1: Normal Irrigation; T2: One Irrigation; T3: Two Irrigation (1st at earthing and 2nd after 30 days)

38

Table 5.12 Effect of irrigation and clones/varieties on tubers yield (t/ha)

Bogra

TCRC/CIP

clones/variety

Marketable Yield (t/ha) Mean

Non-Marketable Yield

(t/ha) Mean

Yield (t/ha)

Mean

T1 T2 T3 T1 T2 T3 T1 T2 T3

LB 1 (391004.18) 24.8 21.0 24.8 23.53 1.2 1.9 0.9 1.312 26.0 22.9 25.7 24.84

LB 2 (391046.14) 30.7 26.1 27.8 28.17 1.7 2.1 1.1 1.615 32.3 28.2 28.8 29.79

LB 3 (391058.175) 24.5 22.0 22.4 22.96 1.7 1.7 1.3 1.543 26.2 23.7 23.6 24.51

LB 7 (393371.58) 33.0 31.9 28.2 31.02 1.3 1.9 1.1 1.453 34.3 33.8 29.3 32.47

LB 9 (396004.337) 32.0 27.2 23.5 27.57 1.3 1.2 3.8 2.118 33.3 28.4 27.3 29.69

LB 12 (396031.119) 25.3 23.4 23.9 24.19 1.3 1.1 1.0 1.142 26.6 24.5 25.0 25.33

LB 14 (396244.12) 26.9 28.8 29.4 28.39 0.6 1.0 0.9 0.866 27.5 29.9 30.4 29.25

Diamnat 27.1 23.9 20.1 23.68 1.4 1.3 0.9 1.216 28.6 25.2 21.0 24.90

Lalshil 7.3 6.1 8.5 7.31 2.2 2.4 2.7 2.439 9.5 8.5 11.3 9.75

Lalpakri 16.1 13.0 12.7 13.93 1.6 2.1 1.9 1.873 17.8 15.1 14.6 15.80

Mean 24.8 22.3 22.1 1.4 1.7 1.6 26.2 24.0 23.7

Statistics S Em ± CD0.05 S Em ± CD0.05 S Em ± CD0.05

Irrigation (I) 4.40 1.61 1.03 0.56 4.63 1.75

Clone/variety (V) 2.94 2.93 0.64 1.02 2.95 3.20

Interaction I × V 2.09 5.07 0.91 1.77 2.27 5.53

T1: Normal Irrigation; T2: One Irrigation; T3: Two Irrigation (1st at earthing and 2nd after 30 days)

39

Table 5.13 Effect of irrigation and clones/varieties on tubers yield (t/ha)

Dinajpur

TCRC/CIP clones/variety Marketable Yield (t/ha)

Mean

Non-Marketable Yield

(t/ha) Mean

Total Yield (t/ha)

Mean

T1 T2 T3 T1 T2 T3 T1 T2 T3

CIP-101(301024.14) 46.44 40.87 42.89 43.40 1.18 1.13 0.85 1.05 47.62 42.00 43.73 44.45

CIP-102 (301029.18) 30.12 30.44 21.33 27.30 0.74 1.00 3.58 1.77 30.86 31.45 24.91 29.07

CIP-111 (380583.8) 35.33 27.65 26.73 29.91 2.11 3.51 7.51 4.38 37.44 31.16 34.24 34.28

CIP-117 (386292.3) 39.11 37.92 32.67 36.57 1.27 2.07 3.22 2.19 40.38 40.00 35.89 38.76

CIP-124 (392781.1) 37.89 32.22 33.11 34.41 0.78 0.96 2.22 1.32 38.67 33.18 35.33 35.73

CIP-126 (392797.22) 43.11 42.22 43.44 42.92 0.71 0.38 2.22 1.10 43.82 42.60 45.67 44.03

CIP-129 (393536.13) 26.11 22.22 25.11 24.48 0.38 2.02 3.67 2.02 26.49 24.25 28.78 26.50

CIP-130 (393617.1) 36.30 30.94 32.12 33.12 0.63 1.58 4.60 2.27 36.93 32.52 36.72 35.39

CIP-134 (395183.7) 45.78 31.11 39.78 38.89 1.11 0.85 4.58 2.18 46.89 31.96 44.36 41.07

CIP-137 (395193.6) 32.22 18.32 23.88 24.81 1.48 0.96 7.41 3.28 33.70 19.28 31.28 28.09

CIP-139 (396311.1) 32.22 26.67 27.89 28.93 1.56 1.16 3.00 1.91 33.78 27.82 30.89 30.83

Diamant 37.36 18.89 26.22 27.49 1.95 4.78 8.34 5.02 39.31 23.67 34.55 32.51

Lalshil 11.56 7.33 9.67 9.52 1.55 1.44 2.26 1.75 13.11 8.78 11.93 11.27

Lalpakri 22.22 14.44 20.89 19.18 2.00 2.36 2.78 2.38 24.22 16.80 23.67 21.56

Mean 33.98 27.23 28.98 1.25 1.73 4.02 35.23 28.96 33.00

Statistics SEm ± CD0.05 SEm ± CD0.05 SEm ± CD0.05

Irrigation (I) 1.02 0.73 0.53 0.23 3.13 2.21

Clone/variety (V) 1.18 1.58 0.47 0.50 3.89 4.77

Interaction I × V 1.13 2.73 0.19 0.86 3.40 8.95

T1: Normal Irrigation; T2: One Irrigation; T3: Two Irrigation (1st at earthing and 2nd after 30 days)

40

Annexure-A

Table A: Monthly Environmental information from November, 09 to March, 2010 at

different locations of Bangladesh

Items Chittagong

Nov’09 Dec’09 Jan’10 Feb’10 March’10

Temperature (0c) Max. 31.33 24.30 27.02 30.40 33.03

Min. 20.60 15.60 13.70 16.90 22.80

Humidity 76.9 75.23 71.20 63.01 69.70

Rainfall (mm) 43.20 0.00 0.00 8.80 59.90

Bogra

Temperature (0c) Max. 30.30 25.60 22.30 28.40 33.60

Min. 18.80 13.20 13.50 14.40 20.10

Humidity 74.10 78.90 73.40 52.50 54.00

Rainfall (mm) 0.00 0.00 0.00 0.00 0.00

Dinajpur

Temperature (0c) Max.

Min.

Rainfall (mm)