CHE 5480 Summer 2005 5FG

• Introduction to nanotechnology and supercomputing.

• Instructors: Lloyd L. Lee (lle@ou.edu)• Gerald K. Newman (gknewman@ou.edu)

• Henry Neeman (hneeman@ou.edu)• Web page:

coecs.ou.edu/lllee/www/nanocourse2005.html• Class sponsored by National Science Foundation (CISE/EIA)

Instructors

What to learn:

• Lectures on nanotechnology

• Learn high performance computing

• Wet labs

• Attend nanotechnology meeting

Projects:

• Chemical and biosensors

• Nanobiotechnology

• Nanostructures and applications:

• =dendrimers, carbon nanotubes, zeolites, aerogels, nanowires, nanoparticles.

• Nanofluidics

• Nanocomposites (heat management)

• Superhydrophobic surfaces—friction/drag reduction

• Laboratory-on-a-chip

• Homeland security, (others)

Textbook:

• Eric Drexler, "Engines of Creation" (Anchor Books, 1987)

• Downloadable from Foresight.Org:

• URL http://www.foresight.org/EOC

Web Pagecoecs.ou.edu/lllee/www/nano2005.html

Grading:

• Homeworks 10%

• Midterm Report 20%

• Midterm Presentation 10%

• Midterm Exam 20%

• Final Report 40%

• No final written test.

Wet Lab:

Nanotechnology Meeting:Houston, Texas, July 28, 2005

James R. Baker Jr. University of Michigan

                                                   

Professor, Internal Medicine and Bioengineering

Chief, Division of AllergyDirector, Center for Biologic

Nanotechnology Co-Director, Center for

Biomedical EngineeringBiotechnology, Nanotechnology and Immunology

Drug Delivery• Research in the area of autoimmune endocrine

disease. He has helped define the basis of the autoimmune response to thyroid auto antigens.

Gene Delivery• Work concerning gene transfer; developing a new

vector system for gene transfer using synthetic polymers (dendrimers).

Anti-microbial research• Work on preventing pathogens from entering the

human body. This research project seeks to develop a composite material that will serve as a pathogen avoidance barrier and post-exposure therapeutic agent to be applied in a topical manner to the skin and mucous membranes.

Drug Delivery by dendrimers

Project called “smart Bombs”:

Target cancerous cells and leave the normal intact.

• Recognition and diagnosis of cancer

• Drug delivery• Location of c cells• Kill by releasing agents

Dendrimers• Known for several applications• Able to enter cells• Little toxicityFocus:• High energy lasers or sound wave

energy to trigger the release of the drug out of the dendrimer.

Drug Delivery by dendrimers

Antimicrobial Nanoemulsion

• Use of soybean oil emulsified with surfactants. Drops ~400 – 600 nm.

• The droplet do not coalesce with themselves . High surface tension make them coalesce with other lipid droplets, killing bacteria.

• Safe for external use. Not safe for red cells, or sperm.

• The droplets fuse with cell membrane of microorganisms resulting in cell lysis.

• Very effective in killing:

– Bacteria, 

– Bacterial spores, 

– Enveloped viruses, and

– Fungal spores.

• They are effective at preventing illness in individuals, when used both before and after exposure to the infective agent.

• They could be used: 

– Topically, 

– As an inhalant.

• Left: treated with nanoemulsion,

• Right: untreated.• The growth of

bacteria colonies has been eliminated by treatment with nanoemulsion.

Antimicrobial Nanoemulsion

Gene Transfer

• G-5 dendrimers of Poly(amidoamine)

• The dendrimer is acetyladed to increase solubility.

• Fluorescein is incorporated onto the dendrimer for imaging in vivo.

• Folic acid is then conjugated as targeting agent.

• The final step is to conjugate the therapeutic drug.

• Into cardiovascular tissues for treatment.

• Use of dendrimer/DNA complexes

–Uniform size, high density, soluble, stable.

• Direct injection or intracoronary delivery.

Gene Transfer

Enhanced expression of beta-galactosidase in electroporated nonvascularized grafts.

A. Graft treated as in group 12, Figure 1. B. Graft treated as in group 4, Figure 1.

(Original magnification    40).