cerise experiment “ c.elegans rnai space experiment”
DESCRIPTION
Instructor:. Toru Mori Takashi Yamasaki. ([email protected]) ([email protected]). - PowerPoint PPT PresentationTRANSCRIPT
1 Training Purposes OnlyJEM CERISE 31407JMU-081217 (Ver. 1.1)
Notice : This technical data is furnished on the condition that it will be used by and disclosed to the receiving Cooperating agency and its contractors and sub contractors only for the purposes of fulfilling the cooperating agency’s responsibilities under the Space Station Intergovernmental Agreement (IGA) and Memorandum of Understanding (MOU). It shall not be used for any other purpose, nor disclosed or retransferred to any other entity or government without prior written permission of the Japan Aerospace Exploration Agency (JAXA).
CERISE Experiment“C.elegans RNAi space experiment”
JEM CERISE 31407
Instructor: Toru Mori Takashi Yamasaki
2 Training Purposes OnlyJEM CERISE 31407JMU-081217 (Ver. 1.1)
Objectives
Perform the CERISE Experiment Operations according to ODFs without instructor’s assistance.
CERISE Experiment Explain the science objectives of the CERISE experiment.
List onboard outcomes from performing the CERISE experiment.
After this lesson the trainee will be able to:
Operation Flow
Outline the CERISE operations flow from the view point of the sample movement.
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Lesson Outline
CERISE Experiment . . . . . . . . . . . . . . . . . . 4
Operation Flow of CERISE . . . . . . . . . . . . . . . . 6
Key Points for CERISE Operations . . . . . . . . . . . 8
Appendix
1. Specification of Meas Exp Unit A . . . . . . . . . . . . . . . . . . . 11
2. Schematic of Meas Exp Unit A . . . . . . . . . . . . . . . . . . . 12
3. Photo of Meas Exp Unit A . . . . . . . . . . . . . . . . . . . 13
4. Acronyms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
4 Training Purposes OnlyJEM CERISE 31407JMU-081217 (Ver. 1.1)
CERISE Experiment
CERISE Experiment (PI: Atsushi Higashitani)
Research Objectives1) Investigate whether RNAi occurs in space as the same manner as on the earth.2) Explore effects of microgravity on entire gene and protein expressions, and phosphoryl
ations beyond generations.
Onboard Outcomes from the Experiment
Cultivated nematodeDosimeterTemp LoggerMicrogravity environment data (taken by MMA)
ReturnedContained in Culture Bag Holder
Downlinked
Microgravity Measurement Apparatus (MMA)
Culture Bag Holder
Returned
5 Training Purposes OnlyJEM CERISE 31407JMU-081217 (Ver. 1.1)
Intentionally Blank
6 Training Purposes OnlyJEM CERISE 31407JMU-081217 (Ver. 1.1)
MELFI
Operation Flow of CERISE
Saibo Rack/CBEF Activation
Meas Exp Unit A (3) Retrieve
Saibo Rack/CBEF Deactivation
CBEF
Cultivation(4 days)
-80 deg C
Sample Preparation
Meas Exp Unit A (4) Instl into CBEF
MLT and MMA Deactivation
MMAM
icro
-G e
nviro
nmen
t m
easu
rem
ent
Sample Retrieve
MLT and MMA Activation
If they are off
As required
ULF3(TBD)
FLIGHT ULF3
CB Setup
CB Stow
CB
CB Activation
Microscope Observation
CB Deactivation
Microscope Observation
:Crew operations
:Crew/Ground cooperative operations
LEGEND:
:Ground operations
Meas Exp Unit A (2) Retrieve
Sample RetrieveMeas Exp Unit A (1) re-installation with dummy mass
Cultivation(4 days) Stow
Stow
Return
Meas Exp Unit A
Culture Bag Holder
ULF3
Launch
7 Training Purposes OnlyJEM CERISE 31407JMU-081217 (Ver. 1.1)
Operation Flow of CERISE (cont’d)
Incubator Unit 1G
Incubator Unit Micro-G
Dummy Mass
nematode
nematode
Culture Bag Holder (4)
Pins
Remove
MELFI(-80 deg C)
Sample Preparation
Meas Exp Unit A (4)
Install
Cultivation (4 days)
Incubator Unit 1G
Incubator Unit Micro-G
CBEF
nematode nematode
nematode nematode
Sample Retrieve
Culture Bag x 15Dosimeter x 1Temp Logger x 1
Temp Logger
Rtrv
Rtrv
Rtrv
Stow
Stow
Stow
Culture Bag
Microscope Observation
Retrieve one Culture BagFrom each
Attach
Observe
Stow
Meas Exp Unit A Re-Instl with dummy mass
Culture Bag Holder(no nematode contained)
Install
CBEF
Re-Instl
Cultivation (4 days)
Sample Retrieve Microscope Observation
Clear Plate
CB Microscope
Install
Reuse
Dummy Mass
Reuse
Stow
8 Training Purposes OnlyJEM CERISE 31407JMU-081217 (Ver. 1.1)
Key Points for CERISE Operations (CBEF)
NOTE: Underlined key points have been covered by CBEF Advanced Training.
Category Equipment Key Points
Crew Safety
Electrical Shock Sample Tray,
Centrifuge
Before attaching/detaching the Canisters to/from the Sample Tray/ Centrifuge, turn off power to Canister by switches on the Sample Tray/Centrifuge.
Entanglement with rotating device
Incubator Unit 1G
Verify visually through the Observation Window that Centrifuge is not rotating before opening the door of the Incubator Unit 1G.
Centrifuge When attaching/detaching the Canisters to/from the 1G Centrifuge, lock turntable of the Centrifuge.
Time Critical Operation N/A N/A
Dos and Don’ts Incubator Unit Do NOT touch inside Incubator Unit with bare hand to keep it clean.
Sample Tray,
Centrifuge
Be careful not to pinch fingers when releasing drawer locks of slide rails on Sample Tray/Centrifuge.
Difficulty in Ops N/A N/A
Possible Mis-Ops N/A N/A
<CBEF>
9 Training Purposes OnlyJEM CERISE 31407JMU-081217 (Ver. 1.1)
Key Points for CERISE Operations (CB)
NOTE: Underlined key points have been covered by CB Advanced Training.
Category Equipment Key Points
Crew Safety
Crew entrapment Air-tight Chamber
When CB is in ‘Stowed Configuration’ fill gaps between the OC and Outer Chassis with Gap Protectors to prevent crew entrapment.
Crew Exposure to Broken Glass
Operation Chamber
Verify no broken glass in Operation Chamber before opening OC door.
Time Critical Operation N/A N/A
Dos and Don’ts Air-tight Chamber
Pull out/stow Air-tight Chamber slowly at about 10 cm per minute to avoid damage to bellows.
OC Camera Raise OC Camera (Lens and Flexible Arm) above the inner plate before stowing the Operation Chamber.
Difficulty in Ops N/A N/A
Possible Mis-Ops N/A N/A
<CB>
10 Training Purposes OnlyJEM CERISE 31407JMU-081217 (Ver. 1.1)
Appendix
Appendix
1. Specification of Meas Exp Unit A
2. Schematic of Meas Exp Unit A
3. Photo of Meas Exp Unit A
4. Acronyms
11 Training Purposes OnlyJEM CERISE 31407JMU-081217 (Ver. 1.1)
Specification of Meas Exp Unit A
Item Specification
Size 239 x 166 x 102 mm
Weight 1585 g
Power +15V, -15V
+12V (for future experiments)
Temp Sensor 20~45 deg C
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Schematic of Meas Exp Unit A
CBEFCircuit Board
Analog (A)
(0~5V)
Analog (B)
(0~5V)
Temperature Sensor (1)
Temperature Sensor (2)
+12V
+15V
-15V
NTSC
ShieldConnector for future
experiments
Jumper
SEL
Relay circuit
Relay circuit
Amplifier circuit
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Overview
Inside
Photo of Meas Exp Unit A
14 Training Purposes OnlyJEM CERISE 31407JMU-081217 (Ver. 1.1)
Acronyms
CBCBEFMELFIMLTMMAOCRNAi
Clean BenchCell Biology Experiment FacilityMinus Eighty degree Celsius Laboratory Freezer for ISSMMA Laptop TerminalMicrogravity Measurement ApparatusOperation ChamberRibonucleic Acid Interference