biologic lp chromatography system - lp chromatography system. table of contents ... chapter 3.0...

Download BIOLOGIC LP CHROMATOGRAPHY SYSTEM - LP CHROMATOGRAPHY SYSTEM. Table of Contents ... Chapter 3.0 System Connections ... 4.2 Adjusting the Platen and Plumbing The Peristaltic Pump

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  • INSTRUCTION MANUAL

    Catalog Numbers731-8300731-8301

    BIOLOGIC LP

    CHROMATOGRAPHY SYSTEM

  • Table of Contents

    TABLE OF CONTENTS

    SafetyGetting Started Quickly

    Chapter 1.0 Introduction...................................................................................................................11.1 Overview.........................................................................................................................11.2 Features .........................................................................................................................21.3 Unpacking.......................................................................................................................31.4 Description of System Components...............................................................................3

    Chapter 2.0 Description of Components ........................................................................................62.1 BioLogic LP ....................................................................................................................62.2 Proportioning Valve/Mixer Module..................................................................................92.3 UV Detector....................................................................................................................102.4 Conductivity Detector .....................................................................................................112.5 Bio-Rad System Options ................................................................................................12

    2.5.1 Model 2110 Fraction Collector ............................................................................122.5.2 Model 2128 Fraction Collector ............................................................................132.5.3 Model 1327 Chart Recorder................................................................................14

    2.6 Non-Bio-Rad System Options ........................................................................................152.6.1 Non-Bio-Rad Fraction Collectors.........................................................................152.6.2 Non-Bio-Rad Chart Recorders............................................................................16

    Chapter 3.0 System Connections ....................................................................................................17

    Chapter 4.0 System Plumbing .........................................................................................................224.1 General Guidelines for Plumbing the System ................................................................224.2 Adjusting the Platen and Plumbing The Peristaltic Pump..............................................244.3 Plumbing the System .....................................................................................................264.4 Purging the System........................................................................................................30

    Chapter 5.0 System Operation.........................................................................................................315.1 Front Panel Controls.......................................................................................................315.2 Manual Mode Operation.................................................................................................34

    5.2.1 Manual Mode Operation of the Pump.................................................................345.2.2 Manual Mode Operation of the Fraction Collector ..............................................385.2.3 Manual Mode Operation of the Alarms ...............................................................415.2.4 Manual Mode Operation of the UV Monitor ........................................................425.2.5 Manual Mode Operation of the Conductivity Monitor..........................................445.2.6 Manual Mode Operation of the Valves ................................................................465.2.7 Manual Mode Operation of the Chart Recorder..................................................48

    5.3 Programming Mode........................................................................................................495.3.1 Programming Modes Main Menu .......................................................................515.3.2 Creating a New Method ......................................................................................535.3.3 Viewing and Editing a Method ............................................................................565.3.4 Program Modes Pump Table ..............................................................................585.3.5 Program Modes Fraction Collector Table............................................................62

    5.3.5.1 Collect All Mode ...................................................................................645.3.5.2 Threshold Collection Mode...................................................................65

  • Table of Contents

    5.3.5.3 Collection Windows Mode ....................................................................675.3.5.4 Threshold and Collection Windows Mode............................................70

    5.3.6 Programming Modes Alarm Table ......................................................................625.3.7 Entering Method Names .....................................................................................75

    5.4 Run Mode.......................................................................................................................765.4.1 Starting a Run .....................................................................................................76

    5.4.1.1 Errors Which Prevent the Start of Runs...............................................785.4.1.2 Using the Delay Feature.......................................................................78

    5.4.2 Run in Progress ..................................................................................................795.4.2.1 Information Available during a Run ......................................................805.4.2.2 Holding a Run.......................................................................................815.4.2.3 Pausing a Run......................................................................................825.4.2.4 Manual Override ...................................................................................83

    5.4.3 Interpreting the Chart Recorder Trace ................................................................84

    Chapter 6.0 Maintenance and Troubleshooting .............................................................................856.1 Cleaning and Storage.....................................................................................................856.2 Pump Calibration............................................................................................................85

    6.2.1 Nominal Calibration.............................................................................................866.2.2 User Calibration ..................................................................................................86

    6.3 Flushing/Cleaning Valves, Flow Cells, and Filters .........................................................876.3.1 Rinsing Valves and Flow Cells............................................................................876.3.2 Cleaning Valves and Flow Cells..........................................................................876.3.3 Cleaning the UV Optics Modules Filters ............................................................87

    6.4 Care of the Proportioning Valve and Mixer ....................................................................886.5 Replacing the Lamp in the UV Optics Module ...............................................................886.6 Troubleshooting..............................................................................................................90

    Appendix A. Specifications ...............................................................................................................92Appendix B. Warranty and Ordering Information ...........................................................................94

    LIST OF FIGURES1. BioLogic LP System .......................................................................................................................12. Proportioning valve/Mixer module ..................................................................................................93. UV Optics Module...........................................................................................................................104. Conductivity Flow Cell and Separate Holder..................................................................................115. Model 2110 Fraction Collector........................................................................................................126. Model 2128 Fraction Collector .......................................................................................................137. Model 1327 Chart Recorder ...........................................................................................................148. Pin Assignments for BioLogic LPs Fraction Collector and Chart Recorder Connectors ...............159. Example of a BioLogic LP System .................................................................................................1710. Rack Assembly ...............................................................................................................................1811. System Cable Connections ............................................................................................................2012. Luer Fittings...........

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